A system for collecting, transferring, culturing and packaging living abyssal macroorganisms with high fidelity

By designing a living abyss macrobiology collection system that includes a long-term life support system, incubator, collector, transporter and sealed connection system, the problem of high mortality rate during the abyss macrobiology collection process is solved, and efficient and portable full-process fidelity collection and cultivation is achieved.

CN116439189BActive Publication Date: 2025-04-08ZHEJIANG UNIV
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Patent Information

Application Number
CN202310472685.9
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2023-04-27
Publication Date
2025-04-08
Estimated Expiration
2043-04-27

AI Technical Summary

Technical Problem

The existing deep-sea sampling equipment is not suitable for abyss macroorganisms, which leads to accidental death of macroorganisms during the collection process and cannot conduct live research.

Method used

A living abyss macrobiological fidelity collection, transfer and culture partition system is designed, including a long-term life support system, incubator, collector, transporter and sealing connection system. The booster circuit is composed of a booster circuit using a booster pump, a nutrition supply mechanism, a booster pipeline and a high-pressure relief valve to achieve movement and independent sealing of the inner cabin.

Benefits of technology

This system can effectively reduce the overall installation volume, improve portability, reduce operation difficulty, and greatly improve the survival rate of Abyss Macrobiology, realizing full-process fidelity collection and cultivation.

✦ Generated by Eureka AI based on patent content.

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Abstract

The present invention relates to the field of marine equipment, and aims to provide a system for collecting, transferring, culturing and packaging living abyssal macroorganisms with fidelity. The system includes a long-term life support system, an incubator, a collector, a transporter and a sealing connection system; wherein the incubator, the collector and the transporter have substantially the same main structure, and all include an outer cabin and an inner cabin of a cylindrical cavity structure; the inner cabin is nested and installed in the outer cabin, and the two are sealed with a gap fit, and when the inner cabin is displaced axially in the outer cabin, the through holes of the two can be overlapped or misaligned, so as to realize the switching of the inner cabin between the state of being connected to the outside or being independently sealed; the transporter and a suction pipe with a built-in suction pump form a transport assembly. The present invention moves the inner cabin and realizes independent sealing by changing the pressure difference, avoiding large-volume sealing structures such as traditional ball valves; the intensive design of the system can greatly reduce the volume of the whole package, improve the portability of the system, and reduce the difficulty of on-site layout and operation in actual use.
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Description

Technical Field

[0001] The present invention relates to the field of marine equipment, and more specifically, to a system for collecting, transferring, culturing and packaging living abyssal macroorganisms with high fidelity. Background Art

[0002] The hadal refers to the sea area with a depth of more than 6000m. There are very few organisms that can adapt to this high-pressure and low-temperature sea area. Due to the limitations of survey technology, human exploration and understanding of hadal organisms and genetic resources are also extremely insufficient, and the discovery and utilization of hadal resources are extremely immature. The detection of hadal macrobiological samples can help humans understand the evolution of hadal life and the survival mechanism in extreme environments, which is of great significance to the fields of engineering and medicine.

[0003] However, due to the current lack of equipment for collecting, transferring and cultivating hadal macroorganism systems, the existing deep-sea sampling equipment is not suitable for hadal macroorganisms, and the actual sampling process often leads to the accidental death of macroorganisms. The macroorganism samples obtained can only be used for genetic research, and it is impossible to study the life characteristics of living hadal macroorganisms such as breathing and movement.

[0004] Therefore, if a system suitable for the faithful collection, transfer, culture and packaging of living abyssal macroorganisms can be proposed, its research value and research methods will be greatly expanded. Summary of the invention

[0005] The technical problem to be solved by the present invention is to overcome the deficiencies in the prior art and provide a system for the fidelity collection, transfer, culture and packaging of living abyssal macroorganisms.

[0006] To solve the technical problem, the solution of the present invention is:

[0007] Provided is a living abyss macro-biological fidelity collection, transfer, culture and packaging system, including a long-term life support system, an incubator, a collector, a transporter and a sealed connection system; wherein,

[0008] The long-term life support system includes an artificial seawater tank, an air compressor, a booster pump, a cooler and a nutrient supply mechanism; the outlet and inlet of the booster pump are respectively connected to the cooler and the artificial seawater tank, the outlet of the air compressor is simultaneously connected to the booster pump and the artificial seawater tank, and the outlet of the cooler is connected to the nutrient supply mechanism; multiple booster pipelines are led out from the nutrient supply mechanism and are respectively connected to the inlet and outlet of the booster pipeline in the incubator, collector or transporter;

[0009] The incubator, collector and transporter have basically the same main structure, all of which include an outer cabin and an inner cabin of a cylindrical cavity structure; wherein the inner cabin is nested and installed in the outer cabin and its axial length is smaller than the latter, and the two are in a sealed clearance fit; a group of through holes arranged oppositely are respectively provided in the middle of the outer cabin and the inner cabin along the radial direction, and when the inner cabin is displaced axially in the outer cabin, the through holes of the two can be overlapped or misaligned, so as to realize the switching of the inner cabin between the state of being connected to the outside or being independently sealed; inlet and outlet of the boosting pipeline are provided on both end faces of the outer cabin, which are used to drive the displacement of the inner cabin by adjusting the pressure difference at both ends; inlet and outlet of the boosting pipeline are provided on the end face of the inner cabin, which are used to maintain the internal pressure of the inner cabin stable; a connecting flange is provided on the outer side of the radial through hole of the outer cabin;

[0010] The transporter and a suction tube with a built-in suction pump form a transport assembly; connecting flanges are provided at both ends of the suction tube, which have the same specifications and dimensions as the connecting flanges provided on the incubator, collector and transporter; the sealing connection system includes a plurality of clamps and hatch covers, and the shapes and dimensions of the clamps and hatch covers are matched with the connecting flanges, so as to realize docking sealing or individual sealing between the connecting flanges.

[0011] As a preferred embodiment of the present invention, the booster pump is a gas-liquid booster pump, and an air compressor provides a boost source for the booster pump.

[0012] As a preferred embodiment of the present invention, the nutrient supply mechanism has a chamber for containing artificial seawater; a plurality of sensors are arranged inside the chamber, including at least a pressure sensor, a temperature sensor, a salinity sensor and a pH sensor.

[0013] As a preferred embodiment of the present invention, a high-pressure relief valve is provided on the boosting pipeline connecting the outer cabin and the inner cabin outlet; the boosting pump, the nutrient supply mechanism, the boosting pipeline and the high-pressure relief valve together constitute a boosting circuit for adjusting or maintaining the cabin pressure.

[0014] As a preferred embodiment of the present invention, three annular grooves are provided on the outer surface of the inner chamber at circumferential intervals, and annular sealing rings are provided in the grooves; the relative through holes in the middle of the inner chamber are arranged on the side wall between two adjacent sealing rings, and the cross-section of the through holes is circular.

[0015] As a preferred embodiment of the present invention, a one-way collecting door that opens inward is provided at a through hole in the middle of the inner cabin of the collector; the one-way collecting door has two symmetrically arranged door panels, one end of which is hingedly mounted on the side of the through hole, and the other end is connected to a fixing on the inner wall through a spring.

[0016] As a preferred embodiment of the present invention, a transfer one-way door which opens inward is provided at a through hole in the middle of the inner cabin of the transporter; the transfer one-way door has two symmetrically arranged door panels, one end of which is hingedly mounted on the side of the through hole, and the other end is connected to the fixing part on the inner wall through a spring; a transfer barrier net which can completely cover the through hole is provided at another through hole in the middle of the inner cabin of the transporter.

[0017] As a preferred embodiment of the present invention, an annular sealing ring is provided on the end face of the connecting flange of the outer cabin, and the inner end face of the cabin cover is a stepped boss structure for forming a sealing fit with the connecting flange; a through hole for connecting the boost pipeline is provided on the cabin cover.

[0018] As a preferred solution of the present invention, an acoustic wave probe is provided on the outer end surface of the hatch cover.

[0019] As a preferred embodiment of the present invention, the transporter also includes a detachable fully enclosed insulation cover and a pressure compensator; when used alone, the transporter can be wrapped in the insulation cover, and the inlet and outlet of the boost pipeline on the hatch cover are respectively connected to the pressure compensator and the plug.

[0020] Compared with the prior art, the present invention has the following beneficial effects:

[0021] 1. The present invention utilizes a booster pump, a nutrient supply mechanism, a booster pipeline and a high-pressure relief valve to form a booster circuit, and moves the inner cabin and realizes independent sealing by changing the pressure difference, thereby avoiding the use of large-volume sealing structures such as ball valves in traditional sampling and culture systems; therefore, the intensive design of the system can greatly reduce the volume of the whole package, improve the portability of the system, and reduce the difficulty of on-site layout and operation in actual use.

[0022] 2. The present invention is a heat-insulating and pressure-maintaining operation throughout the entire process, which greatly improves the survival rate of abyssal macro-organisms.

[0023] 3. The present invention can realize various operations of collecting, transferring, culturing and packaging living abyssal macroorganisms with fidelity, truly realizing the fidelity of the entire process and filling the gap in this technical field. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 This is the overall schematic diagram of the system for collecting, transferring, culturing and packaging living Abyss Macroorganisms in a fidelity manner;

[0025] Figure 2 It is a long-term life support system for Abyssal Macro-organisms;

[0026] Figure 3 This is a schematic diagram of the connection between the Abyssal Macro Biological Collector and the Incubator;

[0027] Figure 4 This is a schematic diagram of the docking of the Abyssal Macro Biological Transporter and the incubator;

[0028] Figure 5 This is a schematic diagram of the Abyssal Macro Biological Transporter;

[0029] Figure 6 Flow chart of the experiment of collecting, transferring, culturing and packaging for the Abyss Macro Biology;

[0030] Figure 7 Schematic diagram of the abyss macro-creature collection process.

[0031] The reference signs in the figure are: 1 long-term life support system, 1-1 artificial seawater tank, 1-2 air compressor, 1-3 booster pump, 1-4 cooler, 1-5 nutrition supply mechanism, 2 incubator, 2-1 booster pipeline inlet, 2-2 culture inner cabin, 2-3 booster pipeline outlet, 2-4 culture outer cabin, 3 collector, 3-1 collect bait, 3-2 collect outer cabin, 3-3 collect inner cabin, 3-4 collect one-way door, 4 transporter, 4-1 transport bait, 4-2 transport outer cabin, 4-3 transport one-way door, 4-4 transport inner cabin, 4-5 transport baffle net, 4-6 suction pipe, 4-7 insulation cover, 4-8 pressure compensator, 5 sealing system, 5-1 clamp, 5-2 hatch cover, 5-3 suction pump. DETAILED DESCRIPTION

[0032] The technical solution of the present invention will be described in detail below by way of embodiments with reference to the accompanying drawings. It should be noted that the description of these embodiments is used to help understand the present invention, but does not constitute a limitation of the present invention.

[0033] like Figure 1 As shown, the living abyssal macro-organism fidelity collection, transfer, culture and packaging system of the present invention comprises a long-term life support system 1, an incubator 2, a collector 3, a transporter 4 and a sealing system 5. Among them, the long-term life support system 1 is used to maintain a constant temperature, pressure, salt and acid in the incubator 2, simulate the living environment of the macro-organism and provide nutrition supply; the incubator 2 is used to carry out long-term cultivation of the sampled macro-organisms; the collector 3 is used to collect living macro-organisms on the seabed; the transporter 4 is used to take out a small amount of macro-organism samples from the incubator 2 for research; the sealing system 5 is used to connect, plug and seal various equipment.

[0034] like Figure 2As shown, the medium- and long-term life support system 1 of the present invention comprises an artificial seawater tank 1-1, an air compressor 1-2, a booster pump 1-3, a cooler 1-4, and a nutrient supply mechanism 1-5. The outlet and inlet of the booster pump 1-3 are respectively connected to the cooler 1-4 and the artificial seawater tank 1-1, the outlet of the air compressor 1-2 is simultaneously connected to the booster pump 1-3 and the artificial seawater tank 1-1, and the outlet of the cooler 1-4 is connected to the nutrient supply mechanism 1-5; multiple booster pipelines are led out from the nutrient supply mechanism 1-5, and are respectively connected to the inlet and outlet of the booster pipelines in the incubator 2, the collector 3 or the transporter 4. The air compressor 1-2 outputs gas to the artificial seawater tank 1-1 to provide dissolved oxygen in the tank; the artificial seawater tank 1-1 is filled with artificial seawater with the same salinity, pH value and other chemical components as the original seawater where macroorganisms live; the booster pump 1-3 is a gas-liquid booster pump, which is provided with a boost source by the air compressor 1-2, and the suction pipe is inserted into the artificial seawater tank 1-1 to convert the normal pressure seawater into high pressure seawater; the cooler 1-4 cools the seawater at the outlet of the booster pump 1-3 to the same temperature as the original seawater; the nutrition supplied by the nutrient supply mechanism 1-5 is the nutrients of the original seawater of macroorganisms such as nematodes and original sea soil microorganisms, which are used to add nutrition to the life support system and ensure the output of high pressure and low temperature artificial seawater. At the same time, the nutrient supply mechanism 1-5 is connected with multiple sensors, including pressure sensors, temperature sensors, salinity sensors, pH sensors, etc., which are used to feedback the seawater parameters output by the long-term life support system 1 and provide feedback for subsequent adjustments. In this example, there are 13 booster lines from the nutrition supply mechanism 1-5, which are connected to the left and right sides of the outer cabin, the outer cabin through hole, the inner cabin, and the end cover of each cabin, so as to create a pressure difference, promote the movement of the inner cabin, or maintain high pressure in the cabin.

[0035] In the present invention, the incubator 2, the collector 3 and the transporter 4 have basically the same main structure, and all include an outer cabin and an inner cabin of a cylindrical cavity structure; wherein the inner cabin is nested and installed in the outer cabin and its axial length is smaller than the latter, and the two are in a sealed clearance fit; a group of through holes arranged oppositely are respectively provided in the middle of the outer cabin and the inner cabin along the radial direction, and when the inner cabin is displaced axially in the outer cabin, the through holes of the two can be overlapped or misaligned, so as to realize the switching of the inner cabin between the state of being connected to the outside or being independently sealed; the inlet and outlet of the boosting pipeline are provided on both end faces of the outer cabin, which are used to drive the displacement by adjusting the pressure difference at both ends of the inner cabin; the inlet and outlet of the boosting pipeline are provided on the end face of the inner cabin, which are used to maintain the internal pressure of the inner cabin stable; and a connecting flange is provided on the outer side of the radial through hole of the outer cabin. Considering that the inner and outer cabin bodies are relatively movable, a through hole should be provided on the end face of the outer cabin and the boosting pipeline connecting the inner cabin should be installed therein, and the two should maintain clearance fit and sealing, so as to ensure that the pressure of the outer cabin will not be lost during the movement of the inner cabin.

[0036] like Figure 3As shown, the incubator 2 includes a booster pipeline inlet 2-1, a culture inner cabin 2-2, a booster pipeline outlet 2-3, and a culture outer cabin 2-4. The booster pipeline inlet 2-1 is connected to the long-term life support system 1 through a booster pipeline, and the booster pipeline of the booster pipeline outlet 2-3 is connected to a high-pressure relief valve, which together constitute a booster pipeline for ensuring the stability of the seawater pressure in the cabin. The culture inner cabin 2-2 is a cylindrical hollow shell cabin, and there are three circumferential sealing rings on its cylindrical surface for sealing; two circular through holes are opened in the middle of the culture inner cabin 2-2 (between the two sealing rings on the right side) along the diameter direction for biological entry and exit; the culture outer cabin 2-4 is also a cylindrical hollow shell cabin, and two through holes are provided in the middle of its side in the same way, and the outer side of the through hole is a connecting flange, and an annular sealing ring is provided on its end face. When the inner culture chamber 2-2 is located at the leftmost side of the outer culture chamber 2-4, the through hole of the inner culture chamber 2-2 is connected with the through hole of the outer culture chamber 2-4, so that the macro-organisms can enter and exit unimpeded. When the inner culture chamber 2-2 is located at the rightmost side of the outer culture chamber 2-4, the two sealing rings on the left and right of the through hole completely seal the inner culture chamber 2-2, so that high pressure maintenance can be achieved.

[0037] like Figure 3 As shown, the collector 3 includes a collection bait 3-1, a collection outer cabin 3-2, a collection inner cabin 3-3, and a collection one-way door 3-4. The collection inner cabin 3-3 is similar in structure to the culture inner cabin 2-2; the collection inner cabin 3-3 contains a collection bait 3-1, which is used to trap macro-organisms to enter the collection inner cabin 3-3 through the through hole; the through hole on one side of the collection inner cabin 3-3 is hinged with a collection one-way door 3-4; the collection one-way door 3-4 has two symmetrically arranged door panels, one end of which is hingedly mounted on the side of the through hole, and the other end is connected to the fixing on the inner wall through a spring, which is used to restrict the escape of macro-organisms after entering the collection inner cabin 3-3; the collection outer cabin 3-2 is similar in structure to the culture outer cabin 2-4, and has two through holes on both side end faces that can be connected to the booster pipeline. After the deep-sea sampling is completed, the collector 3 is docked with the incubator 2 to transfer the macro-organisms. The acoustic wave probe installed on the hatch 5 - 2 is used to emit acoustic waves into the collector 3 to drive the macro-organisms to move from the collector 3 to the incubator 2.

[0038] The collection inner cabin 3-3 and the transport inner cabin 4-4 all have a pressurized pipeline extending out of the outer cabin, and the pressurized pipeline is sealed with the bulkhead of the outer cabin. In the process of collection and transport, the inner cabin can be pressurized by the pressurized pipeline.

[0039] like Figure 4As shown, the transporter 4 includes a transport bait 4-1, a transport outer cabin 4-2, a transport one-way door 4-3, a transport inner cabin 4-4, a transport baffle 4-5, and a heat preservation cover 4-7 and a pressure compensator 4-8 used in conjunction with the transporter 4. The transport bait 4-1 is located in the transport inner cabin 4-4, and is used to attract the movement of macro-organisms from the incubator 2 to the transporter 4; the transport outer cabin 4-2 and the transport inner cabin 4-4 are similar in structure to the culture outer cabin 2-4 and the culture inner cabin 2-2; a transport one-way door 4-3 is provided in the through hole on one side of the transport inner cabin 4-4, and a transport baffle 4-5 is provided in the through hole on the other side, which is used to prevent the macro-organisms from being sucked into the suction pipe 4-6; two through holes are provided at both ends of the transport outer cabin 4-2, which can be connected to the booster pipeline.

[0040] The transporter 4 and a suction tube 4-6 with a built-in suction pump 5-3 form a transport assembly; both ends of the suction tube 4-6 are provided with connection flanges, which have the same specifications and dimensions as the connection flanges provided on the incubator 2, the collector 3 and the transporter 4; the sealing connection system includes a plurality of clamps 5-1 and hatches 5-2, whose shapes and dimensions match the connection flanges, and can achieve docking sealing or individual sealing between the connection flanges. When transferring samples, one end of the suction tube 4-6 is connected to the transport outer cabin 4-2, and the other end is connected to the culture outer cabin 2-4. Starting the suction pump 5-3 can cause the water flow in the cabin to be sucked from the incubator 2 to the transporter 4, thereby driving the macroorganisms to move from the incubator 2 to the transporter 4.

[0041] The hatch cover 5-2 is provided at the bottom connection flange of the transporter 4 to ensure that the pressure of the transfer outer cabin 4-2 remains stable during the transfer process. For example, when the transfer inner cabin 4-4 is at the rightmost side of the transfer outer cabin 4-2, high-pressure water is first filled into the channel at the connection flange of the outer cabin from the through hole on the hatch cover 5-2, and then the inner cabin is moved from right to left. Only when the upper and lower channels of the outer cabin and the left and right sides between the outer cabin and the inner cabin are all filled with high-pressure seawater, the transfer outer cabin 4-2 will not experience a pressure drop when the transfer inner cabin 4-4 is moved.

[0042] like Figure 5 As shown, after the transporter 4 is separated from the incubator 2 and the suction pipe 4-6, it is stored in a detachable all-around insulation cover 4-7, and the inlet and outlet of the pressurized pipeline on the hatch 5-2 are connected to the pressure compensator 4-8 and the plug, respectively. In this way, the temperature and pressure of the transporter 4 can be stabilized in a short period of time so that it can be transported to other places.

[0043] The process of using the system of the present invention to perform operations is specifically exemplified as follows: Figure 6 As shown:

[0044] (1) Collection operation

[0045] like Figure 7As shown, the collector 3 without the hatch 5-2 is assembled with another underwater booster pump and transported to the seabed in the abyss. The booster pump is used to pass high-pressure water to the booster pipeline inlet 2-1 on the right side of the collector 3. The overflow pressure of the overflow valve of the booster pipeline outlet 2-3 is slightly greater than the environment, so that the collection inner cabin 3-3 moves to the leftmost side of the collection outer cabin 3-2. At this time, the collection inner cabin 3-3 is connected to the outside world through the through hole. Macroorganisms near the collector 3 will be trapped by the bait and enter the collection inner cabin 3-3. After a period of trapping, the booster pump is used to pass high-pressure water to the booster pipeline inlet 2-1 on the left side of the collector 3. The overflow pressure of the overflow valve of the booster pipeline outlet 2-3 is slightly greater than the pressure on the right side of the environmental collector 3, thereby pushing the collection inner cabin 3-3 to move to the rightmost side of the collection outer cabin 3-2. The collector 3 is lifted from the seabed and transported to the ship. During the transportation process, the booster pump is used to supplement the pressure to the collection inner cabin 3-3 through the booster pipeline.

[0046] (2) Transfer operation

[0047] like Figure 3 As shown, when the sampler is transported to the ship, the end cap 5-2 is covered, and the connecting flange at the other end is connected to the connecting flange of the incubator 2, and sealed with a clamp 5-1. At this time, the culture inner cabin 2-2 is at the rightmost side of the culture outer cabin 2-4, and the interior is filled with high-pressure seawater. The through hole at the other end of the culture outer cabin 2-4 is connected to the hatch cover 5-2. The long-term life support system 1 is used to fill the end cap of the culture outer cabin 2-4, the left side of the culture outer cabin 2-4, the right side of the culture outer cabin 2-4, the culture inner cabin 2-2, the connection section between the incubator 2 and the collector 3, the left side of the collection outer cabin 3-2, the right side of the collection outer cabin 3-2, the hatch cover 5-2 of the collection outer cabin 3-2, and the collection inner cabin 3-3 with high-pressure and low-temperature seawater to maintain the original high pressure and low temperature of the entire equipment. The overflow pressure of the overflow valve on the right side of the culture outer cabin 2-4 and the collection outer cabin 3-2 is adjusted to be slightly higher than that on the left side, and the culture inner cabin 2-2 and the collection inner cabin 3-3 are moved to the left by the pressure difference until the through holes of the two are completely connected. The sonic probe on the hatch 5-2 is used to emit sonic waves to drive the macroorganisms from the collector 3 to the incubator 2. When the macroorganisms have completely moved to the incubator 2, the overflow pressure of the overflow valve on the left side of the culture outer cabin 2-4 and the collection outer cabin 3-2 is adjusted to be slightly higher than that on the right side, and the culture inner cabin 2-2 and the collection inner cabin 3-3 are moved to the right by the pressure difference until the inner cabin and the outer cabin are completely sealed. Adjust the overflow valve, remove the seawater between the culture inner cabin 2-2 and the culture outer cabin 2-4, and between the collection inner cabin 3-3 and the collection outer cabin 3-2, and disconnect the collector 3 from the incubator 2.

[0048] (3) Long-term cultivation

[0049] The two connecting flanges of the incubator 2 are connected to the hatch 5-2. The long-term life support system 1 is used to fill high-pressure seawater into the upper and lower end covers of the culture outer cabin 2-4, the left and right sides of the culture outer cabin 2-4, and the culture inner cabin 2-2. The double layer of high-pressure and low-temperature seawater inside and outside is used to maintain the stability of the environmental temperature and pressure.

[0050] (4)Transshipment and repackaging

[0051] When a specific number of macro-organism samples are needed, the macro-organisms in the incubator 2 need to be transferred to the transporter 4. The transfer process is similar to the transfer operation of the collector 3. The difference is that the suction tube 4-6 needs to be connected to the incubator 2 and the transporter 4, and the incubator 2 and the transporter 4 through holes are completely connected (such as Figure 4 As shown). When the desired macro-organisms swim through the through hole, start the suction pump in the suction tube 4-6 to generate a large suction water flow in the through hole of the incubator 2 toward the transporter 4, and suck the macro-organisms from the incubator 2 to the transporter 4. Then, similar operations are performed as in step (2), disconnecting the incubator 2 and the transporter 4, and then storing the transporter 4 in a detachable fully enclosed insulation cover 4-7. At this time, the pressure compensator 4-8 needs to be connected to the transport inner cabin 4-4 through a booster pipe, and the booster pipe inlet and outlet on the hatch 5-2 are respectively connected to the plug. In this way, the temperature and pressure stabilization of the transporter 4 can be achieved in a short period of time so that it can be transported to other places.

[0052] (5) Biological experiments

[0053] Researchers extracted macroorganisms from Transporter 4 for experiments.

Claims

1. A system for collecting, transferring, culturing and packaging living abyssal macroorganisms with high fidelity, characterized in that: It includes a long-term life support system, an incubator, a collector, a transporter and a sealed connection system; among which, The long-term life support system includes an artificial seawater tank, an air compressor, a booster pump, a cooler and a nutrient supply mechanism; the outlet and inlet of the booster pump are respectively connected to the cooler and the artificial seawater tank, the outlet of the air compressor is simultaneously connected to the booster pump and the artificial seawater tank, and the outlet of the cooler is connected to the nutrient supply mechanism; multiple booster pipelines are led out from the nutrient supply mechanism and are respectively connected to the inlet and outlet of the booster pipelines in the incubator, collector and transporter; The incubator, collector and transporter have basically the same main structure, all of which include an outer cabin and an inner cabin of a cylindrical cavity structure; wherein the inner cabin is nested and installed in the outer cabin and its axial length is smaller than the latter, and the two are in a sealed clearance fit; a group of through holes arranged oppositely are respectively provided in the middle of the outer cabin and the inner cabin along the radial direction, and when the inner cabin is displaced axially in the outer cabin, the through holes of the two can be overlapped or misaligned, so as to realize the switching of the inner cabin between the state of being connected to the outside or being independently sealed; inlet and outlet of the boosting pipeline are provided on both end faces of the outer cabin, which are used to drive the displacement of the inner cabin by adjusting the pressure difference at both ends; inlet and outlet of the boosting pipeline are provided on the end face of the inner cabin, which are used to maintain the internal pressure of the inner cabin stable; a connecting flange is provided on the outer side of the radial through hole of the outer cabin; The transporter and a suction tube with a built-in suction pump form a transport assembly; connecting flanges are provided at both ends of the suction tube, which have the same specifications and dimensions as the connecting flanges provided on the incubator, collector and transporter; the sealing connection system includes a plurality of clamps and hatch covers, and the shapes and dimensions of the clamps and hatch covers are matched with the connecting flanges, so as to realize docking sealing or individual sealing between the connecting flanges.

2. The system according to claim 1, characterized in that The booster pump is a gas-liquid booster pump, and an air compressor provides a booster source for it.

3. The system according to claim 1, characterized in that The nutrient supply mechanism has a chamber for containing artificial seawater inside; a plurality of sensors are arranged inside the chamber, including at least a pressure sensor, a temperature sensor, a salinity sensor and a pH sensor.

4. The system according to claim 1, characterized in that A high-pressure relief valve is provided on the boosting pipeline connecting the outer cabin and the inner cabin outlet; the boosting pump, the nutrient supply mechanism, the boosting pipeline and the high-pressure relief valve together constitute a boosting circuit for adjusting or maintaining the pressure in the cabin.

5. The system according to claim 1, characterized in that Three annular grooves are arranged at intervals along the circumferential direction on the outer surface of the inner chamber, and an annular sealing ring is arranged in the groove; the relative through holes in the middle of the inner chamber are arranged on the side wall between two adjacent sealing rings, and the cross section of the through holes is circular.

6. The system according to claim 1, characterized in that A one-way collecting door which opens inward is provided at a through hole in the middle of the inner cabin of the collector; the one-way collecting door has two symmetrically arranged door panels, one end of which is hingedly mounted on the side of the through hole, and the other end is connected to a fixing on the inner wall through a spring.

7. The system according to claim 1, characterized in that A transfer one-way door that opens inward is provided at a through hole in the middle of the inner cabin of the transporter; the transfer one-way door has two symmetrically arranged door panels, one end of which is hingedly mounted on the side of the through hole, and the other end is connected to the fixing part on the inner wall through a spring; a transfer barrier net that can completely cover the through hole is provided at another through hole in the middle of the inner cabin of the transporter.

8. The system according to claim 1, characterized in that An annular sealing ring is provided on the end face of the connecting flange of the outer cabin, and the inner end face of the hatch cover is a stepped boss structure for forming a sealing fit with the connecting flange; a through hole for connecting the boost pipeline is provided on the hatch cover.

9. The system according to claim 1, characterized in that An acoustic wave probe is arranged on the outer end surface of the hatch cover.

10. The system according to claim 1, characterized in that The transporter also includes a detachable fully enclosing insulation cover and a pressure compensator; when used alone, the transporter can wrap the pressure compensator in the insulation cover, and at this time, the inlet and outlet of the pressurized pipeline on the cabin cover are respectively connected to the pressure compensator and the plug.

Citation Information

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