Inoculation device for growing edible fungi in greenhouse and use method thereof

Abstract

The present invention discloses an inoculation device for greenhouse cultivation of edible fungi and a method for using the same, and relates to the field of edible fungi inoculation devices. The present invention comprises an inoculation chamber equipped with a sterilization component, a bag opening component arranged on one side of the inoculation chamber, an inoculation component movably arranged inside the inoculation chamber, and a conveying component for conveying the inoculated fungus bags to be inoculated to the bag opening component and the bottom of the inoculation chamber; the bag opening component comprises a box component fixedly arranged on one side of the inoculation chamber, two sterilizing bodies and a plurality of cutting knives movably arranged in the middle of the two sterilizing bodies; an inner cavity is opened inside the inoculation chamber, the sterilizing bodies are fixedly arranged on opposite sides of the inner cavity, the sterilizing bodies are soaked with sterilizing water for sterilizing, and are used to sterilize the cutting knives; the present invention uses the cutting knife with sterilizing water to cut a linear opening in the fungus bag to sterilize the fungus bag, and after inoculation is completed, no manual sealing with tape is performed, which can save the sealing operation and improve the inoculation efficiency.

Description

Technical Field

[0001] The present invention relates to the field of edible fungus inoculation devices, and in particular to an edible fungus inoculation device for greenhouse cultivation and a use method thereof. Background Art

[0002] Inoculation is a crucial step in the cultivation of edible fungi, and the key to this process lies in sterilization. Inoculation for greenhouse cultivation of edible fungi involves transferring the target strain of fungi, usually in a solid or liquid state, into a bag containing culture medium, following aseptic techniques.

[0003] In the traditional inoculation of liquid edible fungi, that is, liquid bacteria, it is necessary to manually operate in a sterile box. First, the inoculation port of the fungus bag containing the culture material to be inoculated is drilled out and placed in the sterile box. Then, a sterilized inoculation stick is inserted into the liquid tube to be stained with the liquid bacteria. The inoculation stick is used to send the liquid bacteria into the inoculation port of the fungus bag. Then, the liquid bacteria is smeared on the culture material. After the inoculation is completed, the inoculation stick needs to be placed on an alcohol lamp for sterilization, and finally, the inoculation operation is repeated with the inoculation stick. During this process, the inoculation port drilled out of the fungus bag is easily infected with miscellaneous bacteria, and it is usually necessary to perform a sealing process after the inoculation is completed, which reduces the inoculation efficiency. At the same time, when the inoculation stick is used to smear the liquid bacteria on the culture material, the liquid bacteria is not easy to smear evenly, resulting in uneven growth of the edible fungi, and the liquid bacteria is not completely smeared on the culture material, which is wasted when the inoculation stick is disinfected.

[0004] Therefore, in the existing greenhouse cultivation of edible fungi, when manual inoculation of bacterial liquid is performed, there are problems such as the inoculation port of the fungus bag being easily infected with miscellaneous bacteria, the sealing of the inoculation port affecting the inoculation efficiency, the uneven inoculation of bacterial liquid, and the waste of bacterial liquid during disinfection. For this reason, we propose an inoculation device for greenhouse cultivation of edible fungi and a method for using the same. Summary of the Invention

[0005] The purpose of the present invention is to provide an inoculation device for greenhouse cultivation of edible fungi and a method of use thereof, which can effectively solve the problems raised in the background technology in the existing greenhouse cultivation of edible fungi, such as the problem that the inoculation port of the fungus bag is easily infected with miscellaneous bacteria when manual inoculation of the fungus liquid is performed, the problem that sealing the inoculation port affects the inoculation efficiency, the problem of uneven inoculation of the fungus liquid, and the problem of wasting the fungus liquid during disinfection.

[0006] To solve the above technical problems, the present invention is achieved through the following technical solutions:

[0007] The present invention is an inoculation device for growing edible fungi in greenhouses, comprising an inoculation chamber equipped with a sterilization component, a bag opening component arranged on one side of the inoculation chamber, an inoculation component movably arranged inside the inoculation chamber, and a conveying component for conveying bags of fungi to be inoculated to the bag opening component and below the inoculation chamber;

[0008] The bag opening assembly includes a box assembly fixedly arranged on one side of the inoculation chamber, two sterilizing bodies, and a plurality of cutting knives movably arranged in the middle of the two sterilizing bodies. The box assembly has an inner cavity, and the sterilizing bodies are fixedly arranged on opposite sides of the inner cavity. The sterilizing bodies are soaked with sterilizing water for sterilization, which is used to sterilize the cutting knives. The cutting knives are dipped in sterilizing water. The cutting knives are used to cut a linear opening in the bag. When the cutting knives cut the opening, sterilizing water is applied to both sides above the opening. The opening allows the inoculation assembly to enter the inoculation solution.

[0009] The cutter comes into contact with the sterilization body and becomes stained with sterilizing water. The sterilizing water is preferably disinfectant alcohol, which evaporates easily without leaving any residue and is less likely to damage the edible fungi during inoculation. In this design, the fungus bag is transported directly below the housing assembly. One of the cutters rotates downward, creating a linear opening in the bag. Because the opening is linear, the bag is held in a taut state after inoculation. Once the sides of the opening are tightened, the opening automatically seals, preventing the entry of outside air and airborne bacteria. Therefore, even if the bag is not manually sealed with tape after inoculation, the growth of the edible fungi within the bag is not affected, eliminating the need for manual sealing and improving inoculation efficiency. Applying sterilizing water to both sides of the opening as the cutter creates the opening also helps sterilize the bag opening, preventing bacteria from entering the bag during inoculation. This sterilization process effectively kills bacteria and prevents bacterial entry, thus ensuring a sterile interior. The cutting knife is always covered with sterile water, and after the cutting knife cuts the bag, it re-enters the two sterilization bodies, which facilitates the disinfection and sterilization of the cutting knife, preventing bacteria from being contaminated by the cutting knife and causing contamination of subsequent bags after cutting. This solves the problems of the existing manual inoculation process, which is easy to cause contamination by using sharp objects to open the bag, the problem of low disinfection efficiency, and the need to seal the bag after inoculation, which is cumbersome.

[0010] The inoculation assembly includes a conduit movably arranged in the middle of the inner side of the inoculation chamber, the interior of the conduit is separated into an inoculation chamber and an air guide chamber by a partition layer, a nozzle is fixedly arranged on the inner top of the conduit for spraying water mist bacterial liquid into the inoculation chamber, a notch is opened at the bottom of the conduit, the notch is located just below the inoculation chamber, an exhaust assembly and an air intake assembly are provided on the inoculation chamber, the air inlet end of the exhaust assembly is connected with the upper interior of the air guide chamber, and the air outlet end of the air intake assembly is connected with the upper interior of the inoculation chamber, the exhaust assembly is used to extract air in the fungus bag to form a negative pressure in the fungus bag, and the air intake assembly is used to disperse the water mist bacterial liquid sprayed by the nozzle into the culture medium in the fungus bag, the cooperation of the notch enables the water mist bacterial liquid in the fungus bag to be dispersed into the culture medium in the negative pressure state, so that the bacterial liquid penetrates into the culture medium, and the water mist bacterial liquid immediately adheres to the culture medium after contacting the culture medium, and the gas removed from the bacterial liquid is then sucked into the air guide chamber and extracted by the exhaust assembly;

[0011] The catheter is a flat tubular structure as a whole, and two separation layers are symmetrically arranged inside. After the inoculation is completed, the fungus bag is in a tight state, and after the two sides of the opening are tightened, the opening is automatically sealed.

[0012] In this design, the portion of the catheter with the air guide cavity is inserted into the culture medium within the bag. Alcohol disinfection prevents the catheter from introducing bacteria into the bag. A nozzle sprays a mist of bacterial liquid into the inoculation chamber. The exhaust assembly extracts air from the culture medium through the air guide cavity, and the air inlet assembly blows the mist into the bag. The gaps in the cavity facilitate the dispersion of the mist into the negatively pressurized culture medium, allowing it to penetrate deeper into the medium, reducing contamination and ensuring more uniform distribution. This allows the growing fungi to more easily access nutrients deep within the medium, resulting in better growth. The air inlet assembly injects sterile air, entraining the mist, through the inoculation chamber into the deep culture medium. Because the bacterial liquid is in the form of a mist, it immediately adheres to the medium upon contact, making it easier for the liquid to adhere. The air removed from the liquid is then drawn into the air guide cavity and removed by the exhaust assembly, ensuring minimal liquid waste during inoculation.

[0013] During manual inoculation, it is necessary to repeatedly insert the inoculation stick dipped in the bacterial solution into the bag for inoculation and repeatedly sterilize the inoculation stick. This design eliminates the need for the inoculation stick, solving the problem of uneven bacterial solution application when manually using the inoculation stick and the problem of wasting bacterial solution on the inoculation stick when sterilizing the inoculation stick.

[0014] Preferably, the bag opening assembly also includes a liquid barrier layer fixedly arranged on the inner wall of the inner cavity, a rotating shaft movably arranged inside the liquid barrier layer, and a motor for driving the rotating shaft to rotate. Two liquid barrier layers are provided, which are respectively located on the opposite side walls of the inner cavity. Three cutting knives are fixedly arranged on the circumference of the rotating shaft, and the three cutting knives are evenly spaced on the rotating shaft.

[0015] In this design, the liquid barrier prevents the sterilizing water from adhering to the shaft, making it less likely for the sterilizing water to be disturbed by the shaft during rotation. When not inoculating, the three cutting blades are stored in the sterilization body, preventing them from being contaminated with bacteria.

[0016] Preferably, two leak-proof blocks are symmetrically arranged on opposite sides of the bottom of the inner cavity to prevent the sterilizing water from dripping, and the tops of the leak-proof blocks are inclined and fit with the bottom of the sterilization body.

[0017] In this design, a sterilizing water content detection device is also provided in the leak-proof block to prevent the sterilizing water from dripping due to excessive concentration.

[0018] Preferably, the box assembly includes a positioning box fixedly arranged on one side of the inoculation chamber and an adjustment box clamped on the side of the positioning box away from the positioning box. The inner cavity is opened on the adjacent sides of the positioning box and the adjustment box. A liquid storage tank for storing sterilized water is fixedly arranged above the positioning box, and a first infusion pump for transporting sterilized water to the two sterilized bodies is fixedly arranged inside the positioning box.

[0019] In this design, by opening the positioning box, it is convenient to inspect and maintain the internal structure. The liquid storage tank and the first infusion pump can supply liquid to the sterilized body in time.

[0020] Preferably, a liquid storage tank for storing bacterial liquid and a second infusion pump for delivering bacterial liquid to the nozzle are fixedly provided on the upper end of one side of the inoculation chamber adjacent to the box assembly. The second infusion pump is located on the lower side of the liquid storage tank. The liquid inlet end of the second infusion pump is fixedly connected to the liquid outlet end at the bottom of the liquid storage tank, and the liquid outlet end of the second infusion pump is connected to the liquid inlet end of the nozzle through a pipeline.

[0021] In this design, the outlet of the second infusion pump is connected to the nozzle via a UV-resistant pipe. The liquid reservoir and the second infusion pump deliver a fixed amount of bacterial solution to the nozzle, allowing the nozzle to expel a fixed amount of water mist each time.

[0022] Preferably, the exhaust assembly includes an exhaust pump fixedly arranged on the side of the inoculation chamber away from the box assembly, the air inlet end of the exhaust pump is connected to the upper interior of the air guide cavity through a first hose, the air intake assembly includes an air delivery pump fixedly arranged on the side of the inoculation chamber away from the box assembly, the air outlet end of the air delivery pump is connected to the upper interior of the inoculation cavity through a second hose, a sterilization box is fixedly provided on the side of the inoculation chamber, and the air inlet end of the air delivery pump is connected to the air outlet end of the sterilization box.

[0023] In this design, the first hose and the second hose are treated with UV protection, and the sterilization box is used to filter bacteria in the air to prevent bacteria from entering the interior of the inoculation chamber and causing contamination.

[0024] Preferably, the catheter is a flat tubular structure as a whole, and two separation layers are symmetrically arranged inside.

[0025] In this design, the entire catheter is a flat tubular structure, which avoids tearing and deformation of the opening cut by the knife on the bag, so that the opening of the bag can form a better seal after inoculation.

[0026] Preferably, the conveying assembly includes a conveyor arranged directly below the inoculation chamber and the box assembly, and a plurality of loading blocks fixedly arranged on the outer side of the conveyor belt of the conveyor. The plurality of loading blocks are evenly spaced on the outer side of the conveyor belt of the conveyor, and a placement groove for placing fungus bags is provided on the side of the loading blocks away from the conveyor belt of the conveyor.

[0027] In this design, the distance between two adjacent carrier blocks is the horizontal distance between the cutter and the guide tube, so that when the front fungus bag is inoculated, the back fungus bag can be in the position where the opening is cut by the cutter.

[0028] Preferably, a hydraulic rod is fixedly provided on the top of the inoculation chamber, and the bottom output end of the hydraulic rod passes through the top wall of the inoculation chamber and is fixedly connected to the center of the upper top of the catheter. A fixed tube is fixedly provided on the top of the inoculation chamber, and a limiting axis is movably provided at the bottom of the fixed tube. The bottom of the limiting axis passes through the top wall of the inoculation chamber and is fixedly connected to one side of the upper top of the catheter.

[0029] In this design, the hydraulic rod adjusts the elevation of the catheter, and the fixed tube and the limiting shaft limit the catheter, so that the catheter moves up and down in a stable state.

[0030] A method for using an inoculation device for growing edible fungi in greenhouses comprises the following steps:

[0031] S1. During use, the conveying assembly is used to convey the fungus bag to the bottom of the box assembly. The cutting knife rotates downward from the sterilizer to cut an opening on the top of the fungus bag. During the process of cutting the opening, the sterilizing water on the knife body is attached to both sides of the opening on the fungus bag to sterilize both sides of the opening. After cutting the opening on the fungus bag, the cutting knife continues to rotate into the sterilizer to carry out sterilization.

[0032] S2, the bag with the opening cut out is then transported to the bottom of the catheter, so that the opening of the bag is directly under the catheter, and the catheter moves downward;

[0033] S3. Continue to move the catheter downward so that the bottom portion of the catheter with the air guide cavity is first inserted into the opening of the fungus bag, then the portion with the inoculation cavity is also inserted into the opening of the fungus bag, and finally the portion with the air guide cavity is inserted into the culture medium in the fungus bag, at which point the portion with the inoculation cavity is located above the culture medium;

[0034] S4. Use the exhaust assembly to extract the air from the culture medium in the fungus bag through the air guide cavity, and use the air intake assembly to blow the water mist of the fungus liquid sprayed from the nozzle into the fungus bag. The water mist of the fungus liquid in the fungus bag is dispersed into the culture medium in the negative pressure state.

[0035] The present invention has the following beneficial effects:

[0036] In the existing greenhouse cultivation of edible fungi, when the bacterial liquid is inoculated manually, there are problems such as the inoculation port of the fungus bag is easily infected with miscellaneous bacteria, the sealing of the inoculation port affects the inoculation efficiency, the problem of uneven bacterial liquid inoculation, and the problem of wasting bacterial liquid during disinfection.

[0037] 1. The present invention uses a cutting blade to create a linear opening in the bag as it rotates downward. Since the opening is linear, the bag is held taut after inoculation. With both sides of the opening tightened, the bag automatically seals, preventing air and airborne bacteria from entering. Therefore, even if the bag is not manually sealed with tape after inoculation, the growth of the edible fungi in the bag is not affected. This eliminates the need for manual sealing and improves inoculation efficiency.

[0038] 2. The present invention uses a cutting knife to cut an opening and then applies sterilizing water on both sides above the opening, which is beneficial for sterilizing the opening of the fungus bag, preventing bacteria from being brought into the fungus bag during inoculation, playing a role in sterilization and preventing bacteria from entering the fungus bag, and is beneficial for aseptic protection of the inside of the fungus bag. Sterile water is always attached to the cutting knife, and the cutting knife re-enters the two sterilization bodies after cutting an opening in the fungus bag, which is beneficial for disinfecting and sterilizing the cutting knife, preventing bacteria from being stained on the cutting knife and causing contamination to subsequent fungus bags after cutting. This solves the problems of easy contamination caused by using sharp objects to break open the fungus bag in existing manual inoculation, the existing problem of low disinfection efficiency, and the need to seal the fungus bag after inoculation, which is cumbersome.

[0039] 3. The present invention uses a nozzle to spray a water mist of bacterial liquid into the inoculation chamber. An exhaust assembly extracts gas from the culture medium within the bag through the air guide chamber. The air inlet assembly then blows the water mist of bacterial liquid sprayed from the nozzle into the bag. The notches allow the water mist of bacterial liquid within the bag to more easily disperse into the negatively pressurized culture medium, allowing it to penetrate deeply into the medium, making it less susceptible to contamination and more evenly dispersed within the medium. This allows the growing edible fungi to more easily access nutrients deep within the medium, resulting in uniform and healthy growth. The air inlet assembly injects sterile gas, carrying the water mist of bacterial liquid through the inoculation chamber and into the deep medium. Since the bacterial liquid is in the form of a water mist, it immediately adheres to the medium upon contact, making it easier for the bacterial liquid to adhere to the medium. The gas removed from the bacterial liquid is then drawn into the air guide chamber and withdrawn by the exhaust assembly, eliminating the waste of bacterial liquid during inoculation. This solves the problem of uneven bacterial solution application when manually inoculating with an inoculation stick, as well as the problem of wasted bacterial solution on the inoculation stick when sterilizing it with an alcohol lamp. Furthermore, this design eliminates the need to cool the inoculation stick after alcohol lamp sterilization, and solves the problem of bacterial solution dying due to incomplete cooling of the inoculation stick, which affects inoculation. BRIEF DESCRIPTION OF THE DRAWINGS

[0040] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for describing the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0041] Figure 1 This is a three-dimensional diagram of an inoculation device for growing edible fungi in greenhouses according to the present invention;

[0042] Figure 2 This is a three-dimensional diagram of an inoculation chamber of an inoculation device for growing edible fungi in greenhouses and the structure arranged thereon;

[0043] Figure 3 This is a three-dimensional diagram of a conduit and the structure provided thereon of an inoculation device for growing edible fungi in greenhouses according to the present invention;

[0044] Figure 4 This is a three-dimensional cross-sectional view of a conduit and a structure provided on the conduit of an inoculation device for growing edible fungi in greenhouses according to the present invention;

[0045] Figure 5 This is a three-dimensional diagram of the internal structure of a bag opening assembly of an inoculation device for growing edible fungi in greenhouses according to the present invention;

[0046] Figure 6 This is a three-dimensional diagram of the rotating shaft and the structure arranged thereon of an inoculation device for growing edible fungi in greenhouses according to the present invention;

[0047] Figure 7 This is a three-dimensional diagram of the position structure of the sterilizing body and the liquid barrier layer of an inoculation device for growing edible fungi in greenhouses according to the present invention;

[0048] Figure 8 This is a front sectional view of an inoculation device for growing edible fungi in greenhouses according to the present invention;

[0049] Figure 9 This is a three-dimensional diagram of the structure of the object carrying block and placement slot of an inoculation device for growing edible fungi in greenhouses according to the present invention.

[0050] In the accompanying drawings, the components represented by the reference numerals are as follows:

[0051] 1. Inoculation chamber; 2. Sterilizer; 3. Cutter; 4. Inner cavity; 5. Catheter; 6. Partition layer; 7. Inoculation chamber; 8. Nozzle; 9. Notch; 10. Liquid barrier; 11. Rotating shaft; 12. Leak-proof block; 13. Positioning box; 14. Adjustment box; 15. Liquid storage box; 16. First infusion pump; 17. Liquid storage tank; 18. Second infusion pump; 19. Exhaust pump; 20. First hose; 21. Air pump; 22. Second hose; 23. Sterilization box; 24. Conveyor; 25. Loading block; 26. Placement slot; 27. Hydraulic rod; 28. Fixed tube; 29. ​​Limiting shaft; 30. Air guide cavity; 31. Motor. DETAILED DESCRIPTION

[0052] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts shall fall within the scope of protection of the present invention.

[0053] In the description of the present invention, it should be understood that the terms "upper", "middle", "outer", "inner" and the like indicating directions or positional relationships are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the components or elements referred to must have a specific direction, be constructed and operate in a specific direction, and therefore should not be understood as limiting the present invention.

[0054] Example:

[0055] See also Figure 1-9 As shown, an inoculation device for growing edible fungi in greenhouses comprises an inoculation chamber 1 equipped with a sterilization assembly, a bag opening assembly arranged on one side of the inoculation chamber 1, an inoculation assembly movably arranged inside the inoculation chamber 1, and a conveying assembly for conveying bags of fungi to be inoculated to the bag opening assembly and below the inoculation chamber 1;

[0056] The bag opening assembly includes a box assembly fixedly arranged on one side of the inoculation chamber 1, two sterilizing bodies 2 and a plurality of cutting knives 3 movably arranged in the middle of the two sterilizing bodies 2. An inner cavity 4 is opened inside the box assembly, and the sterilizing bodies 2 are fixedly arranged on the opposite side of the inner cavity 4. The sterilizing bodies 2 are soaked with sterilizing water for sterilization, which is used to sterilize the cutting knives 3. The cutting knives 3 are dipped in sterilizing water. The cutting knives 3 are used to cut a linear opening in the fungus bag. When the cutting knives 3 cut the opening, sterilizing water is applied on both sides above the opening. The opening allows the inoculation assembly to enter the inoculation solution.

[0057] The cutter 3 comes into contact with the sterilizing body 2 and is coated with sterilizing water. The sterilizing water is preferably disinfectant alcohol, which evaporates easily without leaving any residue and is less likely to damage the edible fungi during inoculation. In this design, the fungus bag is transported directly below the housing assembly. One of the cutters 3 rotates downward to cut a linear opening in the bag. Because the opening in the bag is linear, the bag is in a taut state after inoculation. The tightening of the two sides of the opening automatically seals the opening, preventing outside air and airborne bacteria from entering. Therefore, even if the bag is not manually sealed with tape after inoculation, the growth of the edible fungi in the bag is not affected, eliminating the need for manual sealing and improving inoculation efficiency. Applying sterilizing water to both sides of the opening above the cutter 3 as it creates the opening also facilitates sterilization of the bag opening, preventing bacteria from entering the bag during inoculation. This sterilization process sterilizes and prevents bacteria from entering the bag, thus ensuring a sterile interior. The cutting blade 3 is always coated with sterilizing water, and after the cutting blade 3 cuts the bag, it re-enters the two sterilizing bodies 2, which facilitates the disinfection and sterilization of the cutting blade 3 and prevents bacteria from being contaminated by the cutting blade 3 and causing contamination of subsequent bags after cutting. This solves the problems of the existing manual inoculation process, which is easy to cause contamination by using sharp objects to open the bag, the problem of low disinfection efficiency, and the need to seal the bag after inoculation, which is cumbersome.

[0058] The inoculation assembly includes a conduit 5 movably arranged in the middle of the inner side of the inoculation chamber 1. The interior of the conduit 5 is separated into an inoculation chamber 7 and an air guide chamber 30 by a partition layer 6. A nozzle 8 for spraying water mist bacterial liquid into the inoculation chamber 7 is fixedly arranged on the inner top of the conduit 5. A notch 9 is provided at the bottom of the conduit 5. The notch 9 is located directly below the inoculation chamber 7. An exhaust assembly and an air intake assembly are provided on the inoculation chamber 1. The air inlet end of the exhaust assembly is connected to the upper interior of the air guide chamber 30, and the air outlet end of the air intake assembly is connected to the upper interior of the inoculation chamber 7. The exhaust assembly is used to extract air from the fungus bag to form a negative pressure in the fungus bag, and the air intake assembly is used to disperse the water mist bacterial liquid sprayed by the nozzle 8 into the culture medium in the fungus bag.

[0059] Preferably, the sterilization component is an ultraviolet sterilization lamp, which sterilizes the components in the inoculation chamber 1. In this design, the portion of the conduit 5 with the air guide cavity 30 is inserted into the culture medium in the fungus bag, and the disinfection effect of alcohol prevents the conduit 5 from bringing bacteria into the fungus bag. The nozzle 8 sprays a water mist bacterial solution into the inoculation chamber 7, and the exhaust component is used to extract the gas in the culture medium in the fungus bag through the air guide cavity 30, and the air intake component is used to blow the water mist bacterial solution sprayed by the nozzle 8 into the fungus bag. The cooperation of the notch 9 makes it easier for the water mist bacterial solution in the fungus bag to disperse into the culture medium in a negative pressure state, and also allows the bacterial solution to penetrate into the culture medium, making it less likely to be contaminated, and the bacterial solution is more evenly dispersed in the culture medium, which makes it easier for the edible fungi to obtain nutrients deep in the culture medium when growing, and the edible fungi will grow neatly and grow better. The air inlet assembly injects sterile gas that carries the water mist-like bacterial liquid through the inoculation chamber 7 into the deep of the culture medium. Since the bacterial liquid is in the form of water mist, it will immediately adhere to the culture medium after contacting the culture medium, making it easier for the bacterial liquid to adhere to the culture medium. The gas removed from the bacterial liquid is then sucked into the air guide chamber 30 and extracted by the exhaust assembly, so that no bacterial liquid is wasted during inoculation.

[0060] Manual inoculation requires repeated insertion of an inoculation stick dipped in bacterial solution into the bag for inoculation and repeated sterilization of the stick. This design eliminates the need for an inoculation stick, solving the problems of uneven bacterial solution application when manually using an inoculation stick and the waste of bacterial solution on the stick when sterilizing it with an alcohol lamp. This design also eliminates the need to cool the inoculation stick after alcohol lamp sterilization, solving the problem of bacterial solution dying due to incomplete cooling of the stick, which can affect inoculation.

[0061] Among them, the bag opening component also includes a liquid barrier layer 10 fixedly arranged on the inner wall of the inner cavity 4, a rotating shaft 11 movably arranged inside the liquid barrier layer 10, and a motor 31 for driving the rotating shaft 11 to rotate. Two liquid barrier layers 10 are provided, respectively located on the opposite side walls of the inner cavity 4, and three cutting knives 3 are fixedly arranged on the circumference of the rotating shaft 11, and the three cutting knives 3 are evenly spaced on the rotating shaft 11.

[0062] In this design, the liquid barrier 10 prevents the sterilizing water from adhering to the shaft 11, so that the sterilizing water is not easily disturbed by the shaft 11 during the rotation of the shaft 11. When not inoculated, the three cutting knives 3 will be stored in the sterilization body 2, so that the cutting knives 3 will not be contaminated with bacteria.

[0063] Among them, two leak-proof blocks 12 are symmetrically arranged on opposite sides of the bottom of the inner cavity 4 to prevent the sterilizing water from dripping. The top of the leak-proof block 12 is inclined and fits with the bottom of the sterilization body 2.

[0064] In this design, a sterilizing water content detection device is also provided in the leak-proof block 12 to prevent the sterilizing water from dripping due to excessive concentration.

[0065] Among them, the box assembly includes a positioning box 13 fixedly arranged on one side of the inoculation chamber 1, and an adjustment box 14 clamped on the side of the positioning box 13 away from the positioning box 13. The inner cavity 4 is opened on the adjacent side of the positioning box 13 and the adjustment box 14. A liquid storage tank 15 for storing sterilized water is fixedly arranged above the positioning box 13, and a first infusion pump 16 for transporting sterilized water to the two sterilized bodies 2 is fixedly arranged inside the positioning box 13.

[0066] In this design, by opening the positioning box 13, it is convenient to inspect and maintain the internal structure. The liquid storage tank 15 and the first infusion pump 16 can supply liquid to the sterilized body 2 in time.

[0067] Among them, a liquid storage tank 17 for storing bacterial liquid and a second infusion pump 18 for delivering bacterial liquid to the nozzle 8 are fixedly provided on the upper end of the side of the inoculation chamber 1 adjacent to the box assembly. The second infusion pump 18 is located on the lower side of the liquid storage tank 17. The liquid inlet end of the second infusion pump 18 is fixedly connected to the liquid outlet end at the bottom of the liquid storage tank 17, and the liquid outlet end of the second infusion pump 18 is connected to the liquid inlet end of the nozzle 8 through a pipeline.

[0068] In this design, the liquid outlet of the second infusion pump 18 is connected to the nozzle 8 through a pipe that is UV-resistant. The liquid storage tank 17 and the second infusion pump 18 quantitatively deliver the bacterial solution to the nozzle 8, so that the nozzle 8 can expel the water mist bacterial solution quantitatively each time.

[0069] Among them, the exhaust component includes an exhaust pump 19 fixedly arranged on the side of the inoculation chamber 1 away from the box assembly, and the air inlet end of the exhaust pump 19 is connected to the upper interior of the air guide cavity 30 through a first hose 20. The air intake component includes an air pump 21 fixedly arranged on the side of the inoculation chamber 1 away from the box assembly, and the air outlet end of the air pump 21 is connected to the upper interior of the inoculation cavity 7 through a second hose 22. A sterilization box 23 is fixedly arranged on the side of the inoculation chamber 1, and the air inlet end of the air pump 21 is connected to the air outlet end of the sterilization box 23.

[0070] In this design, the first hose 20 and the second hose 22 are treated with anti-ultraviolet rays, and the sterilization box 23 is used to filter bacteria in the air to prevent bacteria from entering the interior of the inoculation chamber 7 and causing contamination.

[0071] The conduit 5 is a flat tubular structure as a whole, and two separation layers 6 are symmetrically arranged inside.

[0072] In this design, the conduit 5 is a flat tubular structure as a whole, which avoids tearing and deformation of the opening cut by the cutting knife 3 on the fungus bag, so that the opening of the fungus bag can form a better seal after inoculation.

[0073] Among them, the conveying component includes a conveyor 24 arranged directly below the inoculation chamber 1 and the box component, and a plurality of loading blocks 25 fixedly arranged on the outer side of the conveyor belt of the conveyor 24. The plurality of loading blocks 25 are evenly spaced on the outer side of the conveyor belt of the conveyor 24. A placement groove 26 for placing fungus bags is opened on the side of the loading blocks 25 away from the conveyor belt of the conveyor 24.

[0074] In this design, the distance between two adjacent loading blocks 25 is the horizontal distance between the cutter 3 and the guide tube 5, so that when the previous fungus bag is inoculated, the subsequent fungus bag can be in a position where the opening is cut by the cutter 3.

[0075] Among them, a hydraulic rod 27 is fixedly provided on the top of the inoculation chamber 1, and the bottom output end of the hydraulic rod 27 passes through the top wall of the inoculation chamber 1 and is fixedly connected to the top center of the catheter 5. A fixed tube 28 is fixedly provided on the top of the inoculation chamber 1, and a limiting shaft 29 is movably provided at the bottom of the fixed tube 28. The bottom of the limiting shaft 29 passes through the top wall of the inoculation chamber 1 and is fixedly connected to one side of the top of the catheter 5.

[0076] In this design, the hydraulic rod 27 adjusts the lifting and lowering of the catheter 5, and the fixed tube 28 and the limiting shaft 29 limit the catheter 5, so that the upward and downward movement state of the catheter 5 is stable.

[0077] A method for using an inoculation device for growing edible fungi in greenhouses comprises the following steps:

[0078] S1. During use, the conveying assembly is used to convey the fungus bag to the bottom of the box assembly. The cutting knife 3 rotates downward from the sterilizing body 2 to cut an opening on the top of the fungus bag. During the process of cutting the opening, the sterilizing water on the knife body is attached to both sides of the opening on the fungus bag to sterilize both sides of the opening. After cutting the opening on the fungus bag, the cutting knife 3 continues to rotate into the sterilizing body 2 to carry out sterilization.

[0079] S2, the bag with the opening cut out is then transported to the bottom of the guide tube 5, so that the opening of the bag is directly below the guide tube 5, and the guide tube 5 moves downward;

[0080] S3. Continue to move the catheter 5 downward so that the bottom portion of the catheter 5 with the air guide cavity 30 is first inserted into the opening of the fungus bag. Then, the portion with the inoculation cavity 7 is similarly inserted into the opening of the fungus bag. Finally, the portion with the air guide cavity 30 is inserted into the culture medium in the fungus bag. At this point, the portion with the inoculation cavity 7 is located above the culture medium.

[0081] S4. Use the exhaust assembly to extract the air in the culture medium in the fungus bag through the air guide cavity 30, and use the air intake assembly to blow the water mist of the fungus liquid sprayed by the nozzle 8 into the fungus bag, so that the water mist of the fungus liquid in the fungus bag is dispersed into the culture medium in the negative pressure state.

[0082] The preferred embodiments of the present invention disclosed above are intended only to help illustrate the present invention. These preferred embodiments do not exhaustively describe all details, nor do they limit the present invention to the specific embodiments described. Obviously, many modifications and variations are possible based on the content of this specification. These embodiments are selected and described in detail in this specification to better explain the principles and practical applications of the present invention, thereby enabling those skilled in the art to better understand and utilize the present invention. The present invention is limited only by the claims and their full scope and equivalents.

Claims

1. An inoculation device for growing edible fungi in greenhouses, characterized by: The invention comprises an inoculation chamber (1) equipped with a sterilization component, a bag opening component arranged on one side of the inoculation chamber (1), an inoculation component movably arranged inside the inoculation chamber (1), and a conveying component for conveying a bag to be inoculated to the bottom of the bag opening component and the inoculation chamber (1); The bag opening assembly comprises a box assembly fixedly arranged on one side of the inoculation chamber (1), two sterilizing bodies (2) and a plurality of cutting knives (3) movably arranged in the middle of the two sterilizing bodies (2); an inner cavity (4) is opened inside the box assembly; the sterilizing body (2) is fixedly arranged on the opposite side of the inner cavity (4); the sterilizing body (2) is soaked with sterilizing water for sterilization, and is used to sterilize the cutting knives (3); and the cutting knives (3) are dipped in sterilizing water; the cutting knives (3) are used to cut a straight opening in the fungus bag; when the cutting knives (3) cut the opening, sterilizing water is applied to both sides above the opening; the opening is for the inoculation assembly to enter the inoculation liquid; The inoculation assembly comprises a conduit (5) movably arranged in the middle of the inner side of the inoculation chamber (1), the interior of the conduit (5) is separated into an inoculation chamber (7) and an air guide chamber (30) by a partition layer (6), a nozzle (8) for spraying water mist-like bacterial liquid into the inoculation chamber (7) is fixedly arranged on the inner top of the conduit (5), a notch (9) is opened at the bottom of the conduit (5), and the notch (9) is located directly below the inoculation chamber (7), an exhaust assembly and an air intake assembly are arranged on the inoculation chamber (1), and the air intake end of the exhaust assembly is connected to the upper part of the inner side of the air guide chamber (30). The air outlet end of the air inlet component is connected to the upper part of the interior of the inoculation chamber (7), the exhaust component is used to extract the air in the fungus bag to form a negative pressure in the fungus bag, the air inlet component is used to disperse the water mist bacterial liquid sprayed by the nozzle (8) into the culture medium in the fungus bag, the cooperation of the notch (9) allows the water mist bacterial liquid in the fungus bag to be dispersed into the culture medium in the negative pressure state, so that the bacterial liquid penetrates into the culture medium, and the water mist bacterial liquid immediately forms an attachment on the culture medium after contacting the culture medium, and the gas removed from the bacterial liquid is then sucked into the air guide cavity (30) and extracted by the exhaust component; The catheter (5) is a flat tubular structure as a whole, and two partition layers (6) are symmetrically arranged inside. After the inoculation is completed, the fungus bag is in a tight state, and after the two sides of the opening are tightened, the opening is automatically sealed.

2. The inoculation device for growing edible fungi in greenhouses according to claim 1, characterized in that: The bag opening assembly further comprises a liquid barrier layer (10) fixedly arranged on the inner wall of the inner cavity (4), a rotating shaft (11) movably arranged inside the liquid barrier layer (10), and a motor (31) for driving the rotating shaft (11) to rotate. Two liquid barrier layers (10) are provided, which are respectively located on opposite side walls of the inner cavity (4). Three cutting knives (3) are fixedly arranged on the circumference of the rotating shaft (11), and the three cutting knives (3) are evenly spaced and distributed on the rotating shaft (11).

3. The inoculation device for growing edible fungi in greenhouses according to claim 2, characterized in that: Two leak-proof blocks (12) are symmetrically arranged on opposite sides of the bottom of the inner cavity (4) to prevent the sterilizing water from dripping. The tops of the leak-proof blocks (12) are inclined and fit in with the bottom of the sterilizing body (2).

4. The inoculation device for growing edible fungi in greenhouses according to claim 3, characterized in that: The box assembly includes a positioning box (13) fixedly arranged on one side of the inoculation chamber (1), and an adjustment box (14) clamped on the side of the positioning box (13) away from the positioning box (13). The inner cavity (4) is opened on the adjacent sides of the positioning box (13) and the adjustment box (14). A liquid storage tank (15) for storing sterilized water is fixedly arranged above the positioning box (13). A first infusion pump (16) for delivering sterilized water to the two sterilized bodies (2) is fixedly arranged inside the positioning box (13).

5. The inoculation device for growing edible fungi in greenhouses according to claim 4, characterized in that: A liquid storage tank (17) for storing bacterial liquid and a second infusion pump (18) for delivering bacterial liquid to the nozzle (8) are fixedly provided on the upper end of one side of the inoculation chamber (1) adjacent to the box assembly. The second infusion pump (18) is located on the lower side of the liquid storage tank (17). The liquid inlet end of the second infusion pump (18) is fixedly connected to the liquid outlet end at the bottom of the liquid storage tank (17). The liquid outlet end of the second infusion pump (18) is connected to the liquid inlet end of the nozzle (8) through a pipeline.

6. The inoculation device for growing edible fungi in greenhouses according to claim 5, characterized in that: The exhaust assembly includes an exhaust pump (19) fixedly arranged on the side of the inoculation chamber (1) away from the box assembly, the air inlet end of the exhaust pump (19) is connected to the upper part of the interior of the air guide cavity (30) through a first hose (20), the air intake assembly includes an air delivery pump (21) fixedly arranged on the side of the inoculation chamber (1) away from the box assembly, the air outlet end of the air delivery pump (21) is connected to the upper part of the interior of the inoculation cavity (7) through a second hose (22), and a sterilization box (23) is fixedly arranged on the side of the inoculation chamber (1), and the air inlet end of the air delivery pump (21) is connected to the air outlet end of the sterilization box (23).

7. The inoculation device for growing edible fungi in greenhouses according to claim 6, characterized in that: The conveying assembly includes a conveyor (24) arranged directly below the inoculation chamber (1) and the box assembly, and a plurality of load blocks (25) fixedly arranged on the outer side of the conveyor belt of the conveyor (24). The plurality of load blocks (25) are evenly spaced apart on the outer side of the conveyor belt of the conveyor (24), and a placement groove (26) for placing fungus bags is opened on the side of the load blocks (25) away from the conveyor belt of the conveyor (24).

8. The inoculation device for growing edible fungi in greenhouses according to claim 7, characterized in that: A hydraulic rod (27) is fixedly provided on the top of the inoculation chamber (1), and the bottom output end of the hydraulic rod (27) passes through the top wall of the inoculation chamber (1) and is fixedly connected to the center of the upper top of the catheter (5). A fixed tube (28) is fixedly provided on the top of the inoculation chamber (1), and a limit shaft (29) is movably provided at the bottom of the fixed tube (28). The bottom of the limit shaft (29) passes through the top wall of the inoculation chamber (1) and is fixedly connected to one side of the upper top of the catheter (5).

9. A method for using the inoculation device for growing edible fungi in greenhouses according to any one of claims 1 to 8, characterized in that: The following steps are involved: S1. When in use, the conveying assembly is used to convey the fungus bag to the bottom of the box assembly first, the cutting knife (3) rotates downward from the sterilizing body (2), and cuts an opening on the top of the fungus bag. In the process of cutting the opening, the sterilizing water on the knife body is attached to both sides of the opening on the fungus bag to sterilize both sides of the opening. After cutting the opening on the fungus bag, the cutting knife (3) continues to rotate into the sterilizing body (2) to perform sterilization. S2, the bag with the opening cut out is then transported to the bottom of the guide tube (5), so that the opening on the bag is directly opposite to the bottom of the guide tube (5), and the guide tube (5) moves downward; S3, the catheter (5) is further conveyed downward, so that the bottom portion of the catheter (5), which has the air guide cavity (30), is first inserted into the opening of the fungus bag, and then the portion with the inoculation cavity (7) is also inserted into the opening of the fungus bag, and finally the portion of the catheter (5) with the air guide cavity (30) is inserted into the culture medium in the fungus bag, at which point the portion with the inoculation cavity (7) is located above the culture medium; S4. Use the exhaust assembly to extract the air from the culture medium in the fungus bag through the air guide cavity (30), and use the air intake assembly to blow the water mist bacterial liquid sprayed from the nozzle (8) into the fungus bag, so that the water mist bacterial liquid in the fungus bag is dispersed into the culture medium in the negative pressure state.

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