A dual-channel rice planthopper phototaxis experimental method and device

Abstract

The present invention discloses a dual-channel rice planthopper phototaxis experimental method and device, wherein the method includes conducting a single-channel phototaxis rate experiment for light intensity, and conducting a composite phototaxis rate experiment for each light source characteristic except light intensity, to obtain a sensitivity value corresponding to each light source characteristic; the sensitivity value corresponding to the light source characteristic tested first is used as the basic parameter for subsequent experiments. The device includes an experimental box, which has an insect release area and two phototaxis channels; the two phototaxis channels are perpendicular to each other, and a baffle that can separate the first phototaxis channel and the insect release area is provided between the two, and a light source is provided at the end of each of the two phototaxis channels. In the present invention, phototaxis experiments are carried out on rice planthoppers in sequence based on the wavelength, light intensity, shape, frequency and combination of complex colors of light sources to clarify the response characteristics of rice planthoppers to different induced light sources, providing a theoretical basis for further developing high-efficiency insect-attracting lamps for rice planthoppers.

Description

Technical Field

[0001] The present invention relates to the technical field of rice planthopper light attraction research, and in particular to a dual-channel rice planthopper phototaxis experimental method and device. Background Art

[0002] The large-scale and frequent occurrence of rice pests poses a serious threat to rice production. As one of the three major rice pests, rice planthoppers not only directly suck the phloem sap of rice plants with their stylets, but also spread the viruses they carry, causing serious damage to rice yield and quality. According to incomplete statistics, in the years of major outbreaks in Asian rice-growing areas, rice planthoppers infest over 20 million hectares of land, and even after control measures, the losses still reach millions of tons, making them the most serious pest threatening food safety production in the world. Currently, my country's control of rice planthoppers mainly relies on chemical control, but the "3R" problem (Resistance, Resurgence, Residue) caused by long-term application of pesticides is becoming increasingly serious. The resulting food safety and environmental pollution problems have deeply harmed people's daily lives. Therefore, how to carry out precise, efficient, green and pollution-free control of pests has become a hot issue that urgently needs to be addressed in agricultural modernization production.

[0003] Pest light induction technology is a method that uses the insects' phototactic behavior to induce them to gather at a fixed location for centralized elimination. This technology is safe, environmentally friendly, efficient, residue-free, and does not produce resistance. It is a powerful means of green pest control. The key to its efficient application lies in clarifying the pests' phototactic response characteristics to different induced light sources.

[0004] Only by clarifying the phototactic response characteristics of rice planthoppers to different light sources can we effectively and accurately trap them. Currently, research on light-induced trapping technology for rice planthoppers is very insufficient, and their response characteristics to different light sources are still unclear, resulting in low efficiency of field light control.

[0005] In the prior art, patent CN213369472U discloses a multi-channel insect phototaxis test device, which has a resting area, with a reaction area and a light source area on both sides of the resting area, a light source in the light source area, and a light barrier in the resting area. Before the experiment, the light barrier can be lowered to isolate the reaction area from the resting area, and the light barrier can be pulled out during the experiment. The device can provide light sources of different types, wavelengths and intensities to the experimental insects. Patent CN212279506U discloses an insect phototaxis test device, which has a darkroom and three phototaxis channels arranged in a circular array around the darkroom and three collection chambers. The collection chambers are provided with filter boxes and attracting lamps. During operation, filter boxes with different filtering functions are selected according to the purpose of the test. By observing the distribution of insects in each phototaxis channel, the phototaxis can be compared. In addition, patents such as CN217657774U disclose phototaxis behavior test devices with more phototaxis channels.

[0006] The main innovation in the above-mentioned prior art lies in the structure of the testing device. The disclosed testing process is relatively simple and is not conducive to efficiently measuring the response characteristics of different light source characteristics to determine the sensitivity value of each light source characteristic. Therefore, it is necessary to design a reasonable and efficient testing method and a device that can implement this method. Summary of the Invention

[0007] Purpose of the invention: In order to overcome the deficiencies in the prior art, the present invention provides a dual-channel rice planthopper phototaxis experimental method and device, aiming to provide a testing method with a reasonable process and a device for implementing the method, which can efficiently determine the response characteristics of different light source characteristics, so as to determine the sensitivity value of each light source characteristic, and obtain an induced light source that can accurately trap rice planthoppers.

[0008] Technical solution: To achieve the above-mentioned purpose, the dual-channel rice planthopper phototaxis experimental method of the present invention comprises:

[0009] Conduct a single-channel phototaxis experiment for light intensity, and conduct a composite phototaxis experiment for each light source feature except light intensity to obtain the sensitivity value corresponding to each light source feature. The sensitivity value corresponding to the light source feature tested first serves as the basic parameter for subsequent experiments.

[0010] Wherein, the composite phototaxis experiment includes:

[0011] Performing a single-channel phototaxis experiment on the light source characteristic currently being tested to obtain a response curve between the light source characteristic and the first phototaxis;

[0012] According to the response curve, multiple values ​​of the first phototropism rate with the highest value are selected to perform a dual-channel comparison experiment, and thereby obtain the sensitivity value of the light source characteristic currently being tested.

[0013] Furthermore, in addition to light intensity, the light source characteristics also include wavelength, light source shape, light source frequency and complex light combination; the experimental order is: wavelength, light intensity, light source shape, light source frequency and complex light combination.

[0014] Furthermore, in the single-channel phototaxis experiment, the first phototaxis is calculated according to the formula T1=m / n×100%, wherein m is the number of rice planthoppers in an area at a specific distance from the light source, and n is the total number of insects input in a single experiment;

[0015] In the dual-channel comparison experiment, the comparison results are reflected according to the value of the second phototaxis; the second phototaxis is calculated according to the formula T2=m1 / m2×100%; wherein m1 is the number of rice planthoppers in the area at a specific distance from the first light source in the first phototaxis channel; m2 is the number of rice planthoppers in the area at a specific distance from the second light source in the second phototaxis channel.

[0016] Furthermore, in the dual-channel comparison experiment:

[0017] When the number of values ​​to be tested is two, the corresponding light source characteristics of the light sources at the ends of the two phototaxis channels are adjusted directly according to the two values;

[0018] When the number of values ​​to be tested exceeds two, first select two of them, and adjust the light sources at the ends of the two phototaxis channels according to the two selected values ​​to conduct a dual-channel comparison experiment; after completing the dual-channel comparison experiment of these two values, retain the light source corresponding to the value in the dominant position, and adjust the light source corresponding to the value in the disadvantaged position, adjust its corresponding light source characteristics to a new value, and continue the dual-channel comparison experiment; and so on, until all values ​​have been compared.

[0019] Furthermore, each time a single-channel phototaxis experiment or a dual-channel comparative experiment is completed, a new batch of insects is replaced for the next experiment.

[0020] The present invention also provides a dual-channel rice planthopper phototaxis experimental device, which is used to implement the above-mentioned dual-channel rice planthopper phototaxis experimental method, and includes an experimental box, which has three areas, namely an insect release area, a first phototaxis channel and a second phototaxis channel; the first phototaxis channel and the second phototaxis channel are perpendicular to each other, the insect release area is located at the corner position between the two phototaxis channels, a baffle is provided between the first phototaxis channel and the insect release area to separate the two, and when the baffle is removed, the first phototaxis channel and the insect release area are connected; the ends of the two phototaxis channels are respectively provided with a first light source and a second light source.

[0021] Furthermore, the top wall of the experimental box is made of transparent acrylic material, and the other side walls are made of black frosted acrylic material.

[0022] Furthermore, cameras are installed inside the two light-approaching channels.

[0023] Furthermore, the first light source and the second light source are both LED light panels, both of which can be replaced, or both are adjustable light sources, and their parameters such as light intensity, wavelength, frequency, and complex light combination can be changed.

[0024] Furthermore, the LED light board is composed of 30 high-power LEDs and a rectangular foam board, and is powered by a DC power supply.

[0025] Beneficial effects: The dual-channel rice planthopper phototaxis experimental method and device of the present invention have the following beneficial effects:

[0026] (1) For most light source characteristics, a single-channel test is first performed to determine the phototaxis response curve of the light source characteristic. Then, based on the phototaxis response curve, a dual-channel comparison experiment is performed for several values ​​with the highest first phototaxis. In this way, the sensitivity value of each light source characteristic can be effectively determined. The process is reasonable and the test is efficient.

[0027] (2) By rationally sorting the light source characteristics, the sensitivity values ​​of important characteristics such as wavelength and light intensity can be determined first, and then based on these previously determined sensitivity values, the less important light source characteristics can be further determined. In this way, an inducing light source that can accurately trap rice planthoppers can be obtained, thereby facilitating targeted light control and prevention of them;

[0028] (3) The experimental device designed by the present invention has a simple and reasonable structure, which can conveniently carry out single-channel experiments and dual-channel comparative experiments, and effectively avoid the mutual influence of the two light sources during the dual-channel comparative experiment. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 This is the structural diagram of the dual-channel rice planthopper phototaxis experimental device;

[0030] Figure 2 This is the structural diagram of the LED light board;

[0031] Figure 3 Flowchart of the compound phototaxis experiment;

[0032] Figure 4 This is a complete flowchart of the dual-channel rice planthopper phototaxis experiment.

[0033] In the figure: 1-first light source; 2-first camera; 3-baffle; 4-experimental box; 5-second camera; 6-second light source; C1-first phototaxis channel; C2-second phototaxis channel; D-insect release area. DETAILED DESCRIPTION

[0034] The present invention will be further described below with reference to the accompanying drawings.

[0035] like Figure 1 The dual-channel rice planthopper phototaxis experimental device shown is used to implement the dual-channel rice planthopper phototaxis experimental method of the present application, which includes an experimental box 4, which has three areas, namely an insect release area D, a first phototaxis channel C1, and a second phototaxis channel C2; the first phototaxis channel C1 and the second phototaxis channel C2 are perpendicular to each other, the insect release area D is located at the corner between the two phototaxis channels, and a baffle 3 is provided between the first phototaxis channel C1 and the insect release area D to separate the two. When the baffle 3 is removed, the first phototaxis channel C1 and the insect release area D are connected; the ends of the first phototaxis channel C1 and the second phototaxis channel C2 are respectively provided with a first light source 1 and a second light source 6. The insect release area D has an entrance and exit for insects to enter and exit, so as to facilitate the replacement of test insects.

[0036] With this structure, when baffle 3 separates the first phototaxis channel C1 from the insect delivery area D, the aforementioned single-channel phototaxis rate experiment can be conducted through the second phototaxis channel C2. When baffle 3 is raised, the first phototaxis channel C1 is connected to the insect delivery area D, allowing a dual-channel comparison experiment. Specifically, the first light source 1 and the second light source 6 are simultaneously activated to compare the number of rice planthoppers entering the first phototaxis channel C1 and the second phototaxis channel C2, respectively. The L-shaped design of the experimental chamber 4 effectively prevents mutual interference between the first and second light sources 1 and 6 during the dual-channel comparison experiment.

[0037] The top wall of the experimental box 4 is made of transparent acrylic material to facilitate observation of the situation inside the experimental box 4, and the other side walls are made of black frosted acrylic material, which can effectively avoid the influence of light reflection on the experiment.

[0038] In addition, a first camera 2 and a second camera 5 are installed inside the first phototaxis channel C1 and the second phototaxis channel C2, respectively. In this embodiment, the first camera 2 and the second camera 5 are installed at a distance of 630 cm from the first light source 1 and the second light source, respectively. The camera 2 can conveniently record the entire experimental process to obtain more data, facilitate tracing the experimental process, and count the number of rice planthoppers entering the phototaxis channel based on the graph to prevent the crawling of rice planthoppers from affecting the counting operation.

[0039] The first light source 1 and the second light source 6 are both LED light panels, and both can be replaced according to experimental needs, or both are adjustable light sources, and their light intensity, wavelength, frequency, and complex light combination parameters can be changed. Specifically, Figure 2As shown, the LED light panel consists of 30 high-power LEDs (1W / pixel) and a rectangular foam board (30*50cm). Powered by a DC power supply (ITECH-IT6302), the light intensity of the LED light panel can be adjusted by adjusting the current. The light intensity (lux) of the LED light source was calibrated using a digital illuminometer (Aicevoos-AS-V10) at the intersection of phototaxis channels C1 and C2 and insect release area D (70cm from the light source).

[0040] Based on the above experimental device, the dual-channel rice planthopper phototaxis experimental method of the present invention includes:

[0041] Conduct a single-channel phototaxis experiment based on light intensity, and conduct a composite phototaxis experiment based on each light source feature other than light intensity to obtain the sensitivity value corresponding to each light source feature. The sensitivity value of the previously tested light source feature is used as a fixed parameter for subsequent experiments, so that the sensitivity value corresponding to each light source feature can be gradually established.

[0042] Among them, Figure 3 As shown, the composite phototaxis experiment includes the following steps S101-S102:

[0043] Step S101: performing a single-channel phototaxis experiment on the light source characteristic currently being tested to obtain a response curve between the light source characteristic and a first phototaxis;

[0044] In this step, multiple selected values ​​can be taken for the same light source characteristic to conduct a single-channel phototaxis experiment. Taking wavelength as the current light source characteristic being tested as an example, the first phototaxis corresponding to each selected wavelength value is recorded. Finally, each test point is marked in the two-dimensional coordinate system, and curve fitting is performed to obtain the response curve of wavelength and first phototaxis.

[0045] Back to Figure 3 Step S102: According to the response curve, multiple values ​​of the first phototropism rate with the highest value are selected to perform a dual-channel comparison experiment, and thereby obtain the sensitivity value of the light source characteristic currently being tested.

[0046] In the above-mentioned composite phototaxis experiment, a single-channel phototaxis experiment is first performed for specific light source characteristics, and then a dual-channel comparison experiment is performed for the highest few values. This can effectively determine the optimal sensitivity value corresponding to each light source characteristic. The process is reasonable and the test is efficient.

[0047] In addition to light intensity, light source characteristics also include wavelength, shape, frequency, and complex light combinations. The experimental sequence is: wavelength, light intensity, shape, frequency, and complex light combinations. By rationally sorting light source characteristics, we can first determine the sensitivity values ​​of important characteristics such as wavelength and light intensity. Based on these previously determined sensitivity values, we can further determine less important light source characteristics such as shape and frequency. This can lead to an inductive light source that can accurately trap rice planthoppers, thereby facilitating targeted light control and prevention.

[0048] Based on the above-mentioned ranking of light source characteristics, in this application, Figure 4 As shown in the figure, a complete experimental process is as follows:

[0049] Step 1: Conduct a single-channel light source wavelength-first phototaxis response experiment to obtain a wavelength-first phototaxis response curve;

[0050] Step 2: Based on the wavelength-first phototaxis response curve, several wavelengths with the highest first phototaxis were selected for a dual-channel comparison experiment to determine the sensitive wavelengths of rice planthoppers.

[0051] Step 3: Select the sensitive wavelength and conduct a single-channel light intensity-first phototaxis response experiment to obtain the light intensity-first phototaxis response curve and obtain the sensitive light intensity of the rice planthopper;

[0052] Step 4: Select the sensitive wavelength and light intensity, conduct a single-channel light source shape-first phototaxis response experiment, and obtain the shape-first phototaxis response curve;

[0053] Step 5: Based on the shape-first phototaxis response curve, several light source shapes with the highest first phototaxis were selected and a dual-channel comparison experiment was conducted to obtain the sensitive light source shapes of rice planthoppers;

[0054] Step 6: Select the sensitive wavelength, light intensity and shape, conduct a single-channel light source frequency-first phototaxis response experiment, and obtain the frequency-first phototaxis response curve;

[0055] Step 7: Based on the frequency-first phototaxis response curve, select several light source frequencies with the highest first phototaxis, conduct a dual-channel comparison experiment, and obtain the sensitive light source frequency of the rice planthopper;

[0056] Step 8: Select the sensitive wavelength, light intensity, shape and frequency, conduct a single-channel polychromatic light-first phototaxis response experiment, and obtain the polychromatic light-first phototaxis response curve;

[0057] Step 9: Based on the polychromatic light-first phototactic rate response curve, select several polychromatic light combinations with the highest first phototactic rate, conduct a dual-channel comparison experiment, and identify the sensitive polychromatic light combinations of rice planthoppers.

[0058] By combining the above-mentioned sensitive wavelength, sensitive light intensity, sensitive light source shape, sensitive light source frequency and sensitive polychromatic light combination, which correspond to the characteristics of different light sources, the complete parameters of the induced light source are obtained. The induced light source made based on this can achieve good prevention and control effects on rice planthoppers.

[0059] In the single-channel phototaxis experiment, the first phototaxis is calculated according to the formula T1=m / n×100%, wherein m is the number of rice planthoppers in an area at a specific distance (such as 20 cm) from the light source, and n is the total number of insects invested in a single experiment; in the dual-channel comparison experiment, the comparison result is reflected according to the value of the second phototaxis; the second phototaxis is calculated according to the formula T2=m1 / m2×100%; wherein m1 is the number of rice planthoppers in an area at a specific distance from the first light source 1 in the first phototaxis channel C1; m2 is the number of rice planthoppers in an area at a specific distance from the second light source 6 in the second phototaxis channel C2.

[0060] Preferably, in the dual-channel comparison experiment: when the number of tested values ​​is two, the corresponding light source characteristics of the light sources at the ends of the two phototaxis channels are directly adjusted according to the two values; when the number of tested values ​​exceeds two, two values ​​are first selected, and the light sources at the ends of the two phototaxis channels are adjusted according to the two selected values ​​to perform a dual-channel comparison experiment; after completing the dual-channel comparison experiment of these two values, the light source corresponding to the value in the dominant position is retained, and the light source corresponding to the value in the disadvantaged position is adjusted, and its corresponding light source characteristics are adjusted to a new value, and the dual-channel comparison experiment is continued; and so on, until all values ​​have been compared, so that the final sensitivity value can be obtained.

[0061] In the above process, the superior and inferior positions of the two light sources can be determined based on the value of the second phototropism ratio. When the value of the second phototropism ratio is greater than 100%, the first light source 1 is in a superior position and the second light source 6 is in a inferior position; when the value of the second phototropism ratio is less than 100%, the first light source 1 is in a inferior position and the second light source 6 is in a superior position.

[0062] Preferably, after each single-channel phototaxis experiment or dual-channel comparative experiment, a new batch of insects is replaced for the next experiment. All the test insects used in the experiment are cultured together and are only introduced into the experimental box 4 in batches for the experiment. This can avoid the influence of light stimulation on the physiological state of the test insects and cause experimental errors.

[0063] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A dual-channel rice planthopper phototaxis experimental method, characterized in that: The method comprises: Conduct a single-channel phototaxis experiment for light intensity, and conduct a composite phototaxis experiment for each light source feature except light intensity to obtain the sensitivity value corresponding to each light source feature. The sensitivity value corresponding to the light source feature tested first serves as the basic parameter for subsequent experiments. Wherein, the composite phototaxis experiment includes: Performing a single-channel phototaxis experiment on the light source characteristic currently being tested to obtain a response curve between the light source characteristic and the first phototaxis; According to the response curve, multiple values ​​of the first phototropism rate with the highest value are selected to perform a dual-channel comparison experiment, and thereby obtain a sensitivity value of the light source characteristic currently being tested; In the single-channel phototaxis experiment, the first phototaxis is calculated according to the formula T1=m / n×100%, where m is the number of rice planthoppers in an area at a specific distance from the light source, and n is the total number of insects input in a single experiment; In the dual-channel comparison experiment, the comparison result is reflected according to the value of the second phototaxis; the second phototaxis is calculated according to the formula T2=m1 / m2×100%; wherein m1 is the number of rice planthoppers in the area at a specific distance from the first light source (1) in the first phototaxis channel (C1); m2 is the number of rice planthoppers in the area at a specific distance from the second light source (6) in the second phototaxis channel (C2).

2. The dual-channel rice planthopper phototaxis experimental method according to claim 1, characterized in that: In addition to light intensity, the light source characteristics also include wavelength, light source shape, light source frequency and complex light combination; the experimental order is: wavelength, light intensity, light source shape, light source frequency and complex light combination.

3. The dual-channel rice planthopper phototaxis experimental method according to claim 1, characterized in that: In the dual-channel comparison experiment: When the number of values ​​to be tested is two, the corresponding light source characteristics of the light sources at the ends of the two phototaxis channels are adjusted directly according to the two values; When the number of values ​​to be tested exceeds two, first select two of them, and adjust the light sources at the ends of the two phototaxis channels according to the two selected values ​​to conduct a dual-channel comparison experiment; after completing the dual-channel comparison experiment of these two values, retain the light source corresponding to the value in the dominant position, and adjust the light source corresponding to the value in the disadvantaged position, adjust its corresponding light source characteristics to a new value, and continue the dual-channel comparison experiment; and so on, until all values ​​have been compared.

4. The dual-channel rice planthopper phototaxis experimental method according to claim 1, characterized in that: After each single-channel phototaxis experiment or dual-channel comparison experiment, a new batch of insects was replaced for the next experiment.

5. A dual-channel rice planthopper phototaxis experimental device, used to implement the dual-channel rice planthopper phototaxis experimental method according to any one of claims 1 to 4, characterized in that: The experimental box (4) has three areas, namely an insect delivery area (D), a first phototaxis channel (C1), and a second phototaxis channel (C2); the first phototaxis channel (C1) and the second phototaxis channel (C2) are perpendicular to each other, the insect delivery area (D) is located at a corner between the two phototaxis channels, a baffle (3) is provided between the first phototaxis channel (C1) and the insect delivery area (D) to separate the two, and when the baffle (3) is removed, the first phototaxis channel (C1) and the insect delivery area (D) are connected; and a first light source (1) and a second light source (6) are provided at the ends of the two phototaxis channels, respectively.

6. The dual-channel rice planthopper phototaxis experimental device according to claim 5, characterized in that: The top wall of the experimental box (4) is made of a transparent acrylic material, and the other side walls are made of a black frosted acrylic material.

7. The dual-channel rice planthopper phototaxis experimental device according to claim 5, characterized in that: Cameras are installed inside the two phototaxis channels.

8. The dual-channel rice planthopper phototaxis experimental device according to claim 5, characterized in that: The first light source (1) and the second light source (6) are both LED light panels, both of which can be replaced, or both are adjustable light sources, and their light intensity, wavelength, frequency, and complex light combination parameters can be changed.

9. The dual-channel rice planthopper phototaxis experimental device according to claim 8, characterized in that: The LED light board is composed of 30 high-power LEDs and a rectangular foam board, and is powered by a DC power supply.

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