Application of fucoidan oligosaccharide as a nutritional regulator in crops

Abstract

The present invention belongs to the field of modern bio-agriculture, and particularly relates to the application of alginate oligosaccharide as a nutritional regulator in crops. The nutritional regulator is composed of the following active ingredients in parts by weight: 10 parts of alginate oligosaccharide, 2-4 parts of ellagic acid, 4-8 parts of sodium hyaluronate, 5-10 parts of catechin, 5-10 parts of tocopheryl acetate, 5-10 parts of compound amino acid, and 10-20 parts of hydroxyethyl starch. The crops are woody vine plants. The present invention also discloses a preparation method and a specific usage method of the above nutritional regulator. The nutritional regulator of the present invention has low production cost, is convenient for transportation and storage, has no chemical residue, and can effectively promote the yield and disease resistance of woody vine plant economic crops, especially grapes and kiwifruits.

Description

Technical Field

[0001] The present invention belongs to the field of modern bio-agriculture, and particularly relates to the application of alginate oligosaccharide as a nutritional regulator in crops. Background Art

[0002] The use of synthetic pesticides, chemical fertilizers, and crop nutrient regulators has once led to great development in agriculture. However, these chemical reagents have caused the deterioration of the agricultural ecological environment and the decline in the quality of agricultural products, posing a threat to the survival and development of humanity. Traditional crop nutrient regulators include the following: 1. Forchlorfenuron, belonging to the phenylurea class of substances, mainly stimulates cytokinin substances. Forchlorfenuron is a highly active compound with cytokinin activity, which can promote cell division and expansion. When applied to fruit and vegetable plants, it can promote flower bud differentiation, protect flowers and fruits, increase the fruit setting rate, and promote fruit swelling. However, its side effects on humans have gradually been discovered. 2. Naphthaleneacetic acid (NAA), also known as Fengyousu and Maijian. The common preparation is 80% technical powder, and the commercially available formulations also include 99% refined powder, 2% sodium salt aqueous solution, 2% potassium salt aqueous solution, and 4.2% NAA aqueous solution. Naphthaleneacetic acid is an auxin-like substance and a broad-spectrum plant growth regulator. Its main effects on plants are to promote cell division and expansion, induce the formation of adventitious roots, increase fruit setting, prevent fruit drop, change the ratio of male and female flowers, and promote plant metabolism and photosynthesis, accelerate growth and development, and enhance resistance, etc. Naphthaleneacetic acid enters the plant body through the young epidermis of leaves, branches, and seeds and is transported to the acting sites along with the nutrient flow. 3. Gibberellin (GA), also known as Jiuerling and GA. The product formulations used in agricultural production are mostly 85% gibberellin crystal powder, 4% gibberellin emulsifiable concentrate, 40% water-soluble tablets, and 40% water-soluble powder. Gibberellin is a broad-spectrum plant growth regulator. Endogenous gibberellin generally exists in plants and is one of the important hormones promoting plant growth and development. It is also an antagonist of inhibitors such as paclobutrazol and chlormequat chloride. Gibberellin can promote cell elongation, stem elongation, leaf expansion, and promote parthenocarpy and fruit growth, break seed dormancy, change the ratio of male and female flowers, affect flowering time, and reduce flower and fruit abscission. Exogenous gibberellin enters the plant body and has the same physiological effects as endogenous gibberellin. Gibberellin mainly enters the plant body through leaves, young branches, flowers, seeds, or fruits and then conducts to the growth-active sites to play a role. Gibberellin is widely used in agriculture, forestry, and horticulture. 4. Ethephon, also known as Yishiling, Ethephon, and Zaotianhong. Ethephon is slightly toxic to humans and livestock. When used on plants, it can be rapidly absorbed by plant tissues. Since the pH of the plant cell sap > 4.1, ethephon can gradually decompose at the treated site and release ethylene, and it can also be transported in the body and release ethylene at other sites. Ethephon itself has no physiological activity, and the released ethylene is a plant hormone with various physiological functions. The clear physiological effects are: promoting the physiological ripening of fruits (currently, in order to advance the market time of fruits such as bananas, oranges, peaches, and tomatoes in production, ethephon is commonly used), promoting leaf senescence and abscission, promoting seed germination and plant flowering, and promoting the growth of roots and seedlings. If applied improperly, it will cause leaf and fruit abscission, dwarf the plants, change the ratio of male and female, and induce male sterility in some crops, etc.

[0003] Therefore, natural crop nutrient regulators are increasingly being emphasized for use. CN202210507800.7 discloses a laminarin oligosaccharide fertilizer synergist and its preparation method, use, and process, belonging to the technical field of fertilizer preparation. Adding this synergist to fertilizers can induce disease resistance in plants, degrade harmful substances in the soil, stimulate the secretion of auxin, accelerate the plant's metabolism, and improve the plant's disease resistance. It is a mixture prepared from the following raw materials in parts by weight: 12 - 18 parts of laminarin oligosaccharide, 18 - 24 parts of Brevibacillus laterosporus, 5 - 9 parts of Penicillium bilaiae, 7 - 11 parts of Streptomyces rochei, and 8 - 12 parts of Trichoderma harzianum. However, in the above invention, the efficacy of laminarin oligosaccharide requires the cooperation of a large number of active fungi, resulting in high preparation costs, as well as high usage and storage costs. Therefore, a new type of nutrient regulator that is convenient to prepare and easy to store is needed. Summary of the Invention

[0004] To solve the above problems, the present invention discloses the application of laminarin oligosaccharide as a nutrient regulator in crops.

[0005] The present invention includes the following technical solutions:

[0006] The application of laminarin oligosaccharide as a nutrient regulator in crops, wherein the nutrient regulator is composed of the following active ingredients in parts by weight:

[0007]

[0008] The crop is a woody vine.

[0009] Preferably, the nutrient regulator is composed of the following raw materials in parts by weight:

[0010]

[0011] Furthermore, in the above application of laminarin oligosaccharide as a nutrient regulator in crops, the compound amino acid is composed of the following raw materials in parts by weight:

[0012]

[0013] Preferably, the compound amino acid is composed of the following raw materials in parts by weight:

[0014]

[0015] Furthermore, in the above application of laminarin oligosaccharide as a nutrient regulator in crops, the laminarin oligosaccharide is L - polyglucuronic acid.

[0016] Furthermore, in the above application of laminarin oligosaccharide as a nutrient regulator in crops, the nutrient regulator is prepared by the following steps:

[0017] 1) Mixing of active ingredients: weighing brown algal oligosaccharides, ellagic acid, sodium hyaluronate, catechin, tocopherol acetate, compound amino acids, and hydroxyethyl starch by weight; loading into a pulverizing device, uniformly mixing and pulverizing to prepare a granular raw material with a particle size of less than 0.2 mm;

[0018] 2) adding deionized water at a weight ratio of 0.2-0.5 to the granular raw material prepared in step 1), and then adding lanolin at a weight ratio of 0.1-0.2 and glyceryl monostearate at a weight ratio of 0.05-0.15, adding the mixture to a blender and stirring evenly to obtain a paste-like nutritional regulator;

[0019] 3) Put the above nutrition regulator into a barrel-shaped container.

[0020] Furthermore, the above-mentioned brown algae oligosaccharide is used as a nutritional regulator in crops, and the use of the nutritional regulator comprises the following steps:

[0021] a) Using the thick and strong hard branches of the woody vine as cuttings, inserting them into the nutrient regulator and dipping them for 5-10 minutes;

[0022] b) trimming the bottom of the cuttings after dipping into an oblique shape and inserting them into sandy soil with good drainage and ventilation;

[0023] c) After the cuttings have taken root, dip the roots into the nutrient regulator for 20-60 minutes, and then transplant them into fertile, loose, nutrient-rich humus soil.

[0024] Preferably, the woody vine is a grapevine or a kiwi fruit tree.

[0025] Compared with the prior art, the present invention has the following beneficial effects: the creative combination of brown algae oligosaccharide, ellagic acid, sodium hyaluronate and catechin in the formula of the present invention, brown algae oligosaccharide is an important signal molecule in the plant body, which can promote the growth of plants, and can improve the resistance of plants to pests and diseases, and stimulate the plant immune system; hyaluronic acid has a good lubrication, moisturizing and thickening effect, catechin compounds have a 2-phenylchroman structure, belong to flavanol compounds, are easily oxidized by air in aqueous solution, and have good antibacterial and antioxidant effects; ellagic acid has a variety of biologically active functions, such as antioxidant function, but it has not yet been found that ellagic acid is used as a regulator in plant growth. In the embodiments of the present invention, it is found that when brown algae oligosaccharide is used in combination with the above three components at the same time, compared with the use of brown algae oligosaccharide alone, it can greatly promote plant growth and disease resistance, and can be used as a new application method of brown algae oligosaccharide, and play an active role in the increase of production and disease resistance of economic crops such as grapes or kiwifruit. In addition, the nutritional regulator described in the present invention has low production cost, convenient transportation and storage, and no chemical residue. Brief Description of the Drawings

[0026] Figure 1 For the comparison of the yield per mu of grapes in the examples;

[0027] Figure 2 For the comparison of the sugar content and the incidence of gray mold of grapes in the examples;

[0028] Figure 3 For the comparison of the yield per mu of kiwifruit in the examples;

[0029] Figure 4 For the comparison of the sugar content and the incidence of anthracnose of kiwifruit in the examples. Detailed Description of the Invention

[0030] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without making creative efforts shall fall within the protection scope of the present invention.

[0031] Example 1

[0032] The application of fucoidan oligosaccharide as a nutritional regulator in crops, and the nutritional regulator is composed of the following active ingredients in parts by weight:

[0033]

[0034] The fucoidan oligosaccharide is L-polyglucuronic acid;

[0035] The compound amino acid is composed of the following raw materials in parts by weight:

[0036]

[0037] The nutritional regulator is prepared by the following steps:

[0038] 1) Mixing of active ingredients, weighing fucoidan oligosaccharide, ellagic acid, sodium hyaluronate, catechin, tocopheryl acetate, compound amino acid, and hydroxyethyl starch according to parts by weight; loading into a pulverizing device, and uniformly mixing and pulverizing to prepare a granular raw material with a particle size less than 0.2 mm;

[0039] 2) Adding deionized water with a weight ratio of 0.2 - 0.5, wool fat with a weight ratio of 0.1 - 0.2, and glycerol monostearate with a weight ratio of 0.05 - 0.15 to the granular raw material prepared in step 1), and adding to a blender and stirring evenly to obtain a paste-like nutritional regulator;

[0040] 3) Loading the above nutritional regulator into a barrel-shaped container.

[0041] Example 2

[0042] Application of alginate oligosaccharide as a nutrient regulator in crops, wherein the nutrient regulator is composed of active ingredients in the following parts by weight:

[0043]

[0044] The alginate oligosaccharide is L - poly guluronic acid;

[0045] The compound amino acid is composed of raw materials in the following parts by weight:

[0046]

[0047] The nutrient regulator is prepared by the following steps:

[0048] 1) Mixing of active ingredients, weighing alginate oligosaccharide, ellagic acid, sodium hyaluronate, catechin, tocopheryl acetate, compound amino acid, and hydroxyethyl starch according to parts by weight; loading into a pulverizing device, uniformly mixing and pulverizing to prepare particulate raw materials with a particle size less than 0.2 mm;

[0049] 2) Adding deionized water with a weight ratio of 0.2 - 0.5, lanolin with a weight ratio of 0.1 - 0.2, and glycerol monostearate with a weight ratio of 0.05 - 0.15 to the particulate raw materials prepared in step 1), and adding them to a blender and stirring evenly to obtain a paste - like nutrient regulator;

[0050] 3) Loading the above - mentioned nutrient regulator into a barrel - shaped container.

[0051] Example 3

[0052] Application of alginate oligosaccharide as a nutrient regulator in crops, wherein the nutrient regulator is composed of active ingredients in the following parts by weight:

[0053]

[0054] The alginate oligosaccharide is L - poly guluronic acid;

[0055] The compound amino acid is composed of raw materials in the following parts by weight:

[0056]

[0057] The nutrient regulator is prepared by the following steps:

[0058] 1) Mixing of active ingredients, weighing alginate oligosaccharide, ellagic acid, sodium hyaluronate, catechin, tocopheryl acetate, compound amino acid, and hydroxyethyl starch according to parts by weight; loading into a pulverizing device, uniformly mixing and pulverizing to prepare particulate raw materials with a particle size less than 0.2 mm;

[0059] 2) Add deionized water in a weight ratio of 0.2 - 0.5 to the granular raw materials prepared in step 1), then add lanolin in a weight ratio of 0.1 - 0.2 and glycerol monostearate in a weight ratio of 0.05 - 0.15, and add them to a blender and stir evenly to obtain a paste-like nutrient regulator;

[0060] 3) Fill the above-mentioned nutrient regulator into a barrel-shaped container.

[0061] Comparative Example 1

[0062] Application of alginate oligosaccharide as a nutrient regulator in crops, the nutrient regulator is composed of the following active ingredients in parts by weight:

[0063]

[0064]

[0065] The alginate oligosaccharide is L - polyglucuronic acid;

[0066] The compound amino acid is composed of the following raw materials in parts by weight:

[0067]

[0068] The nutrient regulator is prepared by the following steps:

[0069] 1) Mixing of active ingredients, weigh alginate oligosaccharide, tocopheryl acetate, compound amino acid, hydroxyethyl starch according to parts by weight; load them into a pulverizing device, mix and pulverize evenly to prepare granular raw materials with a particle size less than 0.2 mm;

[0070] 2) Add deionized water in a weight ratio of 0.2 - 0.5 to the granular raw materials prepared in step 1), then add lanolin in a weight ratio of 0.1 - 0.2 and glycerol monostearate in a weight ratio of 0.05 - 0.15, and add them to a blender and stir evenly to obtain a paste-like nutrient regulator;

[0071] 3) Fill the above-mentioned nutrient regulator into a barrel-shaped container.

[0072] Comparative Example 2

[0073] Application of alginate oligosaccharide as a nutrient regulator in crops, the nutrient regulator is composed of the following active ingredients in parts by weight:

[0074]

[0075] The alginate oligosaccharide is L - polyglucuronic acid;

[0076] The compound amino acid is composed of the following raw materials in parts by weight:

[0077]

[0078] The nutritional regulator is prepared by the following steps:

[0079] 1) Mixing of active ingredients: Weigh alginate oligosaccharide, sodium hyaluronate, catechin, tocopheryl acetate, compound amino acid, and hydroxyethyl starch by weight; put them into a pulverizing device, and uniformly mix and pulverize to prepare granular raw materials with a particle size less than 0.2 mm.

[0080] 2) Add deionized water with a weight ratio of 0.2 - 0.5, lanolin with a weight ratio of 0.1 - 0.2, and glycerol monostearate with a weight ratio of 0.05 - 0.15 to the granular raw materials prepared in step 1), and add them to a blender and stir evenly to obtain a paste-like nutritional regulator.

[0081] 3) Put the above-mentioned nutritional regulator into a barrel-shaped container.

[0082] Comparative Example 3

[0083] Application of alginate oligosaccharide as a nutritional regulator in crops. The nutritional regulator is composed of the following active ingredients by weight:

[0084]

[0085] The alginate oligosaccharide is L - polyglucuronic acid;

[0086] The compound amino acid is composed of the following raw materials by weight:

[0087]

[0088]

[0089] The nutritional regulator is prepared by the following steps:

[0090] 1) Mixing of active ingredients: Weigh alginate oligosaccharide, ellagic acid, catechin, tocopheryl acetate, compound amino acid, and hydroxyethyl starch by weight; put them into a pulverizing device, and uniformly mix and pulverize to prepare granular raw materials with a particle size less than 0.2 mm.

[0091] 2) Add deionized water with a weight ratio of 0.2 - 0.5, lanolin with a weight ratio of 0.1 - 0.2, and glycerol monostearate with a weight ratio of 0.05 - 0.15 to the granular raw materials prepared in step 1), and add them to a blender and stir evenly to obtain a paste-like nutritional regulator.

[0092] 3) Put the above-mentioned nutritional regulator into a barrel-shaped container.

[0093] Comparative Example 4

[0094] Application of alginate oligosaccharide as a nutrient regulator in crops, wherein the nutrient regulator is composed of the following active ingredients by weight:

[0095]

[0096] The alginate oligosaccharide is L-poly-guluronic acid;

[0097] The compound amino acid is composed of the following raw materials by weight:

[0098]

[0099] The nutrient regulator is prepared by the following steps:

[0100] 1) Mixing of active ingredients: Weigh alginate oligosaccharide, ellagic acid, sodium hyaluronate, tocopheryl acetate, compound amino acid, and hydroxyethyl starch according to the weight parts; put them into a pulverizing device, and uniformly mix and pulverize to prepare granular raw materials with a particle size less than 0.2 mm;

[0101] 2) Add deionized water with a weight ratio of 0.2 - 0.5, lanolin with a weight ratio of 0.1 - 0.2, and glycerol monostearate with a weight ratio of 0.05 - 0.15 to the granular raw materials prepared in step 1), and add them to a stirrer and stir evenly to obtain a paste-like nutrient regulator;

[0102] 3) Put the above-mentioned nutrient regulator into a barrel-shaped container.

[0103] Comparative Example 5

[0104] Application of alginate oligosaccharide as a nutrient regulator in crops, wherein the nutrient regulator is composed of the following active ingredients by weight:

[0105]

[0106] The alginate oligosaccharide is L-poly-guluronic acid;

[0107] The compound amino acid is composed of the following raw materials by weight:

[0108]

[0109] The nutrient regulator is prepared by the following steps:

[0110] 1) Mixing of active ingredients: Weigh ellagic acid, sodium hyaluronate, catechin, tocopheryl acetate, compound amino acid, and hydroxyethyl starch according to the weight parts; put them into a pulverizing device, and uniformly mix and pulverize to prepare granular raw materials with a particle size less than 0.2 mm;

[0111] 2) adding deionized water at a weight ratio of 0.2-0.5 to the granular raw material prepared in step 1), and then adding lanolin at a weight ratio of 0.1-0.2 and glyceryl monostearate at a weight ratio of 0.05-0.15, adding the mixture to a blender and stirring evenly to obtain a paste-like nutritional regulator;

[0112] 3) Put the above nutrition regulator into a barrel-shaped container.

[0113] Test Example 1

[0114] Examples 1-3 and Comparative Examples 1-5 were used in a grape growth experiment, and a commercial comparative example of gibberellin was used as a commercial comparison. The grape variety was Carignano. In the commercial comparative example, 10 PPM of gibberellin solution was sprayed during the budding period.

[0115] And Examples 1-3 and Comparative Examples 1-5 use the following method:

[0116] a) Use the strong and hard branches of grapes as cuttings and insert them into the nutrient regulator and soak them for 5 minutes;

[0117] b) trimming the bottom of the cuttings after dipping into an oblique shape and inserting them into sandy soil with good drainage and ventilation;

[0118] c) After waiting for the cuttings to take root, dip the roots into the nutrient regulator for 20 minutes, and then transplant them into fertile, loose, and nutrient-rich humus soil.

[0119] The growth, results and disease resistance of the grapevines in the above different embodiments were statistically analyzed, and the results are shown in Tables 1 and Figure 1 Figure 2 shown.

[0120] Table 1 Grape yield per mu and disease rate

[0121]

[0122] From Table 1 and Figure 1 Figure 2 It can be seen that when brown algae oligosaccharides are used together with ellagic acid, sodium hyaluronate and catechins, it can greatly promote plant growth and disease resistance compared to using brown algae oligosaccharides alone. It can be used as a new application method of brown algae oligosaccharides and play a positive role in increasing yield and resisting diseases in grape crops.

[0123] Test Example 2

[0124] Examples 1-3 and Comparative Examples 1-5 were used in a growth experiment of kiwifruit, and the commercial comparative example chlorfenapyr was used as a commercial comparison. The kiwifruit variety was Hayward. In the commercial comparative example, in the Hayward sapling stage, buds at suitable positions were selected on the previously grown branches in mid-to-late May, and the buds were dipped in a 20-30 times diluted commercial chlorfenapyr (0.1%) solution and combined with the method of removing the leaves around the buds to promote their rapid germination.

[0125] And Examples 1-3 and Comparative Examples 1-5 use the following method:

[0126] a) Use the thick and strong hard branches of the kiwifruit tree as cuttings and insert them into the nutrient regulator and soak them for 10 minutes;

[0127] b) trimming the bottom of the cuttings after dipping into an oblique shape and inserting them into sandy soil with good drainage and ventilation;

[0128] c) After waiting for the cuttings to take root, dip the roots into the nutrient regulator for 60 minutes, and then transplant them into fertile, loose, and nutrient-rich humus soil.

[0129] The growth, fruiting and disease resistance of the kiwifruit trees in the above different embodiments were statistically analyzed, and the results are shown in Table 2 and Table 3. Figure 3 Figure 4 shown.

[0130] Table 2 Kiwifruit yield per mu and disease rate

[0131]

[0132] From Table 2 and Figure 3 Figure 4 It can be seen that when brown algae oligosaccharides are used together with ellagic acid, sodium hyaluronate and catechins, it can greatly promote plant growth and disease resistance compared to using brown algae oligosaccharides alone. It can be used as a new application method of brown algae oligosaccharides and play a positive role in increasing yield and resisting diseases in kiwifruit crops.

[0133] The above embodiments are limited to several preferred implementations of the present invention, and their descriptions are relatively specific and detailed, but they cannot be understood as limiting the scope of the present invention. It should be pointed out that for ordinary technicians in this field, several modifications and improvements can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention.

Claims

1. Application of alginate oligosaccharide as a nutritional regulator in crops, characterized in that, The nutrient regulator consists of the following active ingredients by weight: The crop is a woody vine; The alginate oligosaccharide is L-poly-guluronic acid; The compound amino acid consists of the following raw materials by weight:

2. The application according to claim 1, characterized in that, The nutrient regulator consists of the following raw materials by weight:

3. The application according to claim 1, characterized in that, The compound amino acid consists of the following raw materials by weight:

4. The application according to claim 1, characterized in that, The nutrient regulator is prepared by the following steps 1) Mixing of active ingredients: Weigh alginate oligosaccharide, ellagic acid, sodium hyaluronate, catechin, tocopheryl acetate, compound amino acid, and hydroxyethyl starch by weight; load them into a pulverizing device and uniformly mix and pulverize to prepare granular raw materials with a particle size less than 0.2 mm; 2) Add deionized water with a weight ratio of 0.2 - 0.5, lanolin with a weight ratio of 0.1 - 0.2, and glycerol monostearate with a weight ratio of 0.05 - 0.15 to the granular raw materials prepared in step 1), and add them to a blender and stir evenly to obtain a paste-like nutrient regulator; 3) Load the above nutrient regulator into a barrel-shaped container.

5. The application according to claim 1, characterized in that The use of the nutrient regulator includes the following steps: a) Use the thick and strong hard branches of the woody vine as cuttings and dip them into the nutrient regulator for 5 - 10 minutes; b) Trim the bottom of the above-dipped cuttings into an inclined mouth shape and insert them into sandy soil with good drainage and ventilation; c) After waiting for the cuttings to take root, dip the roots into the nutrient regulator for 20 - 60 minutes, and then transplant them into fertile, loose, and nutrient-rich humus soil.

6. The application according to claim 1, wherein The woody vine is a grapevine or a kiwifruit tree.

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