Preparation method of composite plant extract and application thereof in heat-clearing rose tea

Abstract

The invention discloses a preparation method of a composite plant extract and application of the composite plant extract in heat-clearing rose tea. The composite plants are corn silk and tartary buckwheat seeds. The invention firstly performs low-temperature rapid freezing treatment on the corn silk and tartary buckwheat seeds, immediately crushes them, and then combines them with high-voltage pulse electric field technology, ultrasonic extraction technology and low-temperature fermentation technology to perform low-temperature extraction throughout the process, thereby avoiding the loss of effective components caused by high-temperature processing and also improving the extraction rate of the effective components. The inventor adopts artificial inoculation of dominant bacteria for fermentation, thereby jointly improving the blood pressure-lowering effects of the corn silk and tartary buckwheat seeds; and by adding an impurity remover, the extract can be well purified and impurities removed, and the light transmittance of the rose tea can be effectively improved, thereby preventing the presence of impurities from affecting the color and taste of the rose tea.

Description

Technical Field

[0001] The invention relates to the technical field of plant extraction, in particular to a preparation method of a composite plant extract and application of the composite plant extract in heat-clearing rose tea. Background Art

[0002] Rose is a woody plant that combines medicinal, edible, beautifying and greening functions. Rose is warm in nature, sweet and slightly bitter in taste, and belongs to the liver and spleen meridians. It is a good medicine for treating gynecological diseases, cardiovascular and cerebrovascular diseases, traumatic injuries, and mental illnesses. The use of rose buds as medicine has a history of hundreds of years. "Compendium of Materia Medica" states: "Rose has the strongest fragrance, which is clear but not turbid, harmonious but not fierce. It can soften the liver and wake up the stomach, regulate qi and activate blood circulation, and relieve stagnation. It has no disadvantages of being pungent, warm, hard and dry. It is the most effective and docile among the qi-regulating medicines, and there is no other aromatic product that can match it." "Compendium of Materia Medica" states: "It can activate blood circulation, regulate qi, and treat rheumatism, breast pain, initial liver swelling and toxins, and liver and stomach qi pain."

[0003] Scented tea is a processed tea unique to my country. Each kind of scented tea contains more or less natural nutrients, which is undoubtedly the icing on the cake for our body. Scented tea is mainly made of green tea, black tea or oolong tea as tea base, with fresh flowers that can emit fragrance as raw materials, and is made by scenting technology. Roses are rich in vitamins A, C, B, E, K, and tannic acid, which can improve endocrine disorders and help eliminate fatigue and wound healing. Regulate qi and blood, regulate female physiological problems, promote blood circulation, beautify, regulate menstruation, diuresis, soothe gastrointestinal nerves, prevent wrinkles, prevent frostbite, beautify the skin, clear away heat and detoxify, reduce swelling and remove acne, and clear liver fire. Rose tea is a high-end tea made by mixing fresh roses and tea buds in proportion and scenting with modern high-tech technology. Its aroma is strong and light, and it is harmonious but not strong. Therefore, more and more people are drinking rose tea now. However, when rose tea is drunk alone, it has the defects of single nutrients and functions.

[0004] CN105454583A discloses a rose tea and a preparation method thereof, wherein the weight ratio of each raw material component is: 100-200 fresh roses, 20-30 bamboo leaves, 10-12 lemons, 1-3 salt, 1-3 bamboo shoots, 3-5 snakeberries, and 2-4 wolfberries. The rose tea prepared in the present invention has the advantages of fragrant aroma and bright color. The rose tea is soaked in a nutrient solution made of bamboo shoots, snakeberries, etc., which also enables the rose tea to have health care effects such as clearing away heat and detoxifying. The rose tea uses more raw materials and has not been extracted. During the soaking process, more impurities will be generated, which will affect the color and taste of the rose tea. Summary of the invention

[0005] In view of the above-mentioned defects of the prior art, the technical problem to be solved by the present invention is to provide a heat-clearing rose tea with good gloss, fragrance, taste and blood pressure lowering effect.

[0006] To achieve the above purpose, the present invention adds a composite plant extract into rose tea, wherein the composite plant is corn silk and tartary buckwheat seeds with the function of clearing away heat and detoxifying, the extraction process is low-temperature extraction throughout the process, thereby avoiding the loss of effective ingredients caused by high-temperature processing, and adopts artificial inoculation of dominant bacteria for fermentation, thereby improving the blood pressure lowering effect of corn silk and tartary buckwheat seeds, and by adding an impurity remover, the extract can be well purified and impurities can be removed, and the light transmittance of the rose tea can be effectively improved, thereby avoiding the presence of impurities affecting the color and taste of the rose tea and giving the rose tea the blood pressure lowering function.

[0007] In order to achieve the above-mentioned object of the invention, the present invention adopts the following technical solutions:

[0008] A method for preparing a composite plant extract comprises the following steps:

[0009] The corn silk and buckwheat seeds were mixed and washed with water, and then sprayed with a 0.5-1.5wt% β-cyclodextrin aqueous solution on the surface, and then allowed to stand for 10-12 hours and freeze-dried at -30 to -20°C for 1-2 hours. After freeze-drying, they were immediately crushed and passed through an 80-100 mesh sieve, and water was added at a solid-liquid ratio of 1:10-15g / mL. Ultrasonic extraction was performed at room temperature, 60-100Hz, and 120-150W for 1-2 hours; then, extraction was performed under a high-voltage pulse electric field for 10-30 min, then adjust the pH to 5-7 with 30-36wt% acetic acid aqueous solution to obtain a mixture, add mixed bacterial solution to the mixture, ferment at 25-30°C for 8-10d, after the fermentation is completed, add impurity remover to the fermentation liquid, stir for 10-20min, filter, evaporate the filtrate under reduced pressure to remove the solvent, pasteurize at 60-80°C for 20-30min, and finally freeze-dry at -30 to -20°C for 6 to 12h to obtain a composite plant extract.

[0010] Preferably, the usage ratio of corn silk, tartary buckwheat seeds and β-cyclodextrin aqueous solution is 500-1000:500-1000:5-10.

[0011] Preferably, the high-voltage pulse electric field extraction conditions are: an electric field strength of 20-40 kV / cm, a pulse time of 400-500 μs, and a pulse frequency of 200-300 Hz.

[0012] Preferably, the mixed bacterial liquid is a mixture of Lactobacillus lentus bacterial liquid and Aureobasidium aureum bacterial liquid in a mass ratio of 1-2:2-3; the added amount of the mixed bacterial liquid is 2-5% of the mass of the mixture.

[0013] Preferably, the method for preparing the Lactobacillus hilarii bacterial liquid comprises the following steps:

[0014] Lactobacillus lentus is inoculated into a culture medium which is a mixture of 2-5wt% glucose, 2-3wt% fish peptone, 1-2wt% yeast extract, 0.1-0.5wt% potassium dihydrogen phosphate, 0.1-0.5wt% calcium carbonate and the balance is water, with an inoculation amount of 1-3wt%, and the culture is carried out at a temperature of 25-35°C for 24-36h to obtain a Lactobacillus lentus bacterial liquid.

[0015] Preferably, the method for preparing the dark golden basidiomycete liquid comprises the following steps:

[0016] The dark golden basidiomycete is inoculated into a culture medium prepared by uniformly mixing 6-10wt% skimmed milk powder, 2-5wt% inulin, 0.5-1.2wt% sodium dihydrogen phosphate, 0.1-1.0wt% magnesium sulfate and the remainder water, with an inoculation amount of 1-2wt%. The culture is carried out at a temperature of 25-35°C for 24-36h to obtain a dark golden basidiomycete bacterial liquid.

[0017] Corn silk is the style and stigma of the grass plant Zea mays L. It contains saponins, volatile oils, fatty acids, alkaloids, flavonoids, polysaccharides, vitamins and minerals and other chemical components. It is mild in nature and sweet in taste. It enters the liver, kidney and bladder meridians and has the effects of diuresis, lowering blood sugar, inhibiting bacteria, lowering blood pressure, enhancing immunity, clearing away heat and detoxifying, promoting dampness and relieving stranguria, and at the same time has hemostatic effects; anti-cancer effects; Tartary buckwheat, also known as bitter buckwheat, is a plant of the Polygonaceae family and the genus Fagopyrum. Its seeds can be used for food or as feed; the root can be used for medicinal purposes, regulating qi and relieving pain, strengthening the spleen and removing dampness. It is also called "intestine cleansing grass" among the people. It has the effects of softening blood vessels, improving circulation, clearing away heat and detoxifying, promoting blood circulation and removing blood stasis, invigorating qi and refreshing the mind, and regulating blood pressure.

[0018] The present invention firstly performs low-temperature rapid freezing treatment on corn silk and bitter buckwheat seeds, immediately crushes them, and then combines them with high-voltage pulse electric field technology, ultrasonic extraction technology, and low-temperature fermentation technology to perform low-temperature extraction throughout the process, thereby avoiding the loss of effective components caused by high-temperature processing, and also improving the extraction rate of effective components. The inventor uses artificial inoculation of dominant bacteria for fermentation, and jointly improves the blood pressure lowering effect of corn silk and bitter buckwheat seeds. Corn silk and bitter buckwheat seeds contain a large amount of active substances such as polysaccharides, flavonoids, polyphenols, amino acids, proteins, organic acids, etc. After being extracted by high-voltage pulse electric field technology and ultrasonic extraction technology, they can effectively promote their growth and mass reproduction under the action of mixed bacterial liquid, and can produce more blood pressure lowering flavonoid substances during the fermentation of corn silk and bitter buckwheat seeds, thereby improving the blood pressure lowering effect of the composite plant extract, and improving its use value. After fermentation, the effective component molecules contained in the extract are smaller, have better affinity, and can be better absorbed by the human body. At the same time, Lactobacillus tarda and Basidiomycetes can promote the conversion of glutamate in corn silk and buckwheat seeds into γ-aminobutyric acid. γ-Aminobutyric acid is an important inhibitory neurotransmitter in the central nervous system, which has the effects of anticonvulsant, promoting brain metabolism, preventing cerebral arteriosclerosis and significantly inhibiting the increase in blood pressure, thereby improving the blood pressure lowering effect of the composite plant extract.

[0019] Preferably, the amount of the impurity remover added is 1-3‰ of the mass of the fermentation liquid.

[0020] Preferably, the preparation method of the impurity remover comprises the following steps:

[0021] 1) Under an argon atmosphere, 100-120 g of glucose and 100-120 g of ammonium chloride are mixed and placed in a tube furnace, heated to 800-900° C. at a heating rate of 200-250° C. / min, then reacted at 800-900° C. for 3-5 hours, cooled to room temperature to obtain a blister, and then the blister is placed in an air atmosphere and oxidized at 300-400° C. for 6-8 hours to obtain a nanocarbon compound;

[0022] 2) adding 10-15 g of nanocarbon compound, 15-20 g of nanocellulose, 20-25 g of sodium alginate and 10-15 g of β-glucan to 50-80 g of water, ultrasonically treating the mixture for 20-30 min at 50-80 Hz and 1500-2000 W, adding 1-3 g of sodium tripolyphosphate, heating the mixture to 80-100° C., reacting the mixture for 2-4 h, cooling the mixture to room temperature, adding 30-50 g of propolis, stirring the mixture at 600-800 rpm for 1-2 h at room temperature, and obtaining a mixed system;

[0023] 3) The mixed system obtained in step 2) is subjected to electrospinning, and a high voltage of 15-20KV is applied during the electrospinning process. The distance between the nozzle of the syringe and the receiving device is 10-15cm to obtain composite nanofibers, and then the composite nanofibers are made into a sheet structure to obtain an impurity remover.

[0024] The present invention also discloses the application of the composite plant extract in heat-clearing rose tea, and the specific application is as follows: fresh roses and plants with heat-clearing function commonly used in the art are dried, mixed with the composite plant extract and black tea, ground into powder and packaged.

[0025] Preferably, the plants with heat-clearing function are a mixture of one or more of Cassia seed, Gallnut, Honeysuckle and mulberry leaf.

[0026] The composite plant extract contains a small amount of tannic acid, protein, pectin, inorganic salts, and pigments, which leads to poor light transmittance and affects the color of rose tea. In addition, the rich products produced by the fermentation metabolism of microbial agents will destroy the balance of the rose tea system. The inventor loads nanofibers with nanocarbon compounds and forms a porous three-dimensional network structure with high porosity and high specific surface area through electrospinning, which can purify the extract and effectively improve the light transmittance of rose tea, avoid the presence of impurities affecting the color of rose tea, and increase the electrospinning process. The contact area with impurities and macromolecular substances, the porous structure of nanocarbon compounds can adsorb heavy metals and miscellaneous bacteria in plant extracts, sodium alginate and β-glucan can promote propolis to form a composite film attached to the surface of materials formed by nanocarbon compounds, nanocellulose, etc., the active groups on the surface of propolis can purify the composite plant extract system, can react with the bitter peroxidized polyphenols in the composite plant extract, and effectively remove the peroxidized polyphenols and unstable pigment substances in the extract, so that the prepared rose tea has better gloss, fragrance, mellow taste and other characteristics.

[0027] Compared with the prior art, the invention has the following beneficial effects: firstly, the invention performs low-temperature rapid freezing treatment on corn silk and tartary buckwheat seeds, immediately crushes them, and then combines them with high-voltage pulse electric field technology, ultrasonic extraction technology, and low-temperature fermentation technology to perform low-temperature extraction throughout the process, thereby avoiding the loss of effective components caused by high-temperature processing and also improving the extraction rate of the effective components; artificial inoculation of dominant bacteria is used for fermentation, thereby jointly improving the blood pressure lowering effects of corn silk and tartary buckwheat seeds; and impurity removal is performed on the extract by treating the extract with an impurity remover, thereby effectively improving the light transmittance of rose tea and improving the blood pressure lowering effect of the rose tea. DETAILED DESCRIPTION

[0028] To avoid redundancy, the items used in the following examples are all commercially available products unless otherwise specified, and the methods used are all conventional methods unless otherwise specified.

[0029] The sources of some raw materials used in the present invention are as follows:

[0030] Corn silk, originating from Hubei.

[0031] Buckwheat seeds, origin Ningxia

[0032] Lactobacillus hilgris, Latin name Lentilactobacillus, CICC25208, was purchased from China Industrial Microbiological Culture Collection Center.

[0033] Aureobasidium pullulans, scientific name Aureobasidium pullulans, CICC40320, was purchased from China Industrial Microbiological Culture Collection Center.

[0034] Propolis, concentrated powder, 100 mesh, Baoji Liupanyun Biotechnology Co., Ltd.

[0035] Nanocellulose, content 99.5%, particle size 20-50nm, Wuhan Huaxiang Kejie Biotechnology Co., Ltd.

[0036] Example 1

[0037] A method for preparing a composite plant extract comprises the following steps:

[0038] 500g corn silk and 500g bitter buckwheat seeds were mixed, washed with water, and then sprayed with 5g 1.0wt% β-cyclodextrin aqueous solution on the surface. After standing for 12h, freeze-dried at -20℃ for 2h. After freeze-drying, they were immediately crushed and passed through an 80-mesh sieve. Water was added at a solid-liquid ratio of 1:15g / mL. Ultrasonic extraction was performed at room temperature, 80Hz, and 120W for 2h; then, extraction was performed under a high-voltage pulse electric field with an electric field strength of 30kV / cm, a pulse time of 500μs, and a pulse frequency of 300Hz for 20min, and then 36wt% acetic acid was used to extract the seeds. The solution was adjusted to pH 6 to obtain a mixture, and a mixed bacterial solution of Lactobacillus tarda and Basidiomycetes aureus in a mass ratio of 1:2 was added to the mixture, and the added amount of the mixed bacterial solution was 3% of the mass of the mixture. The mixture was fermented at 30°C for 10 days. After the fermentation was completed, an impurity remover was added to the fermentation liquid in an amount of 5‰ of the mass of the fermentation liquid. The mixture was stirred for 10 minutes and then filtered. The filtrate was decompressed to remove the solvent and then pasteurized at 70°C for 20 minutes. Finally, it was freeze-dried at -20°C for 12 hours to obtain a composite plant extract.

[0039] The preparation method of the Lactobacillus hilarii bacterial liquid comprises the following steps:

[0040] Lactobacillus lentus was inoculated into a culture medium composed of 3wt% glucose, 2.5wt% fish peptone, 1.5wt% yeast extract, 0.3wt% potassium dihydrogen phosphate, 0.2wt% calcium carbonate, and the remainder water, with an inoculation amount of 2wt%. The culture was carried out at 30°C for 36h to obtain a Lactobacillus lentus bacterial liquid.

[0041] The method for preparing the dark golden basidiomycete liquid comprises the following steps:

[0042] The dark golden basidiomycete was inoculated into a culture medium prepared by uniformly mixing 8wt% skimmed milk powder, 3wt% inulin, 0.8wt% sodium dihydrogen phosphate, 0.5wt% magnesium sulfate and the remainder water, with an inoculation amount of 1.5wt%. The culture was carried out at 30°C for 30 hours to obtain a dark golden basidiomycete culture solution.

[0043] The preparation method of the impurity remover comprises the following steps:

[0044] 1) Under an argon atmosphere, 100 g of glucose and 100 g of ammonium chloride were mixed and placed in a tube furnace, heated to 900° C. at a heating rate of 250° C. / min, then reacted at 900° C. for 3 h, cooled to room temperature to obtain a vesicle, and then the vesicle was placed in an air atmosphere and oxidized at 400° C. for 8 h to obtain a nanocarbon compound;

[0045] 2) 12.5 g of nanocarbon compound, 18 g of nanocellulose, 23 g of sodium alginate, and 12.8 g of β-glucan were added to 80 g of water, and ultrasonically treated at 60 Hz and 1800 W for 20 min, and then 2.5 g of sodium tripolyphosphate was added, and the mixture was heated to 100° C., reacted for 3 h, cooled to room temperature, and 40 g of propolis was added. The mixture was stirred and mixed at a speed of 700 rpm for 2 h at room temperature to obtain a mixed system;

[0046] 3) The mixed system obtained in step 2) is subjected to electrospinning, and a high voltage of 18KV is applied during the electrospinning process. The distance between the nozzle of the syringe and the receiving device is 12cm to obtain composite nanofibers, and then the composite nanofibers are made into a sheet structure to obtain an impurity remover.

[0047] Comparative Example 1

[0048] A method for preparing a composite plant extract comprises the following steps:

[0049] 500g corn silk and 500g bitter buckwheat seeds were mixed, washed with water, and then sprayed with 5g 1.0wt% β-cyclodextrin aqueous solution on the surface. After standing for 12h, they were freeze-dried at -20℃ for 2h. After freeze-drying, they were immediately crushed and passed through an 80-mesh sieve. Water was added at a solid-liquid ratio of 1:15g / mL. Ultrasonic extraction was performed at room temperature, 80Hz, and 120W for 2h; then, extraction was performed under a high-voltage pulse electric field with an electric field strength of 30kV / cm, a pulse time of 500μs, and a pulse frequency of 300Hz for 20min. Then, a 36wt% acetic acid aqueous solution is used to adjust the pH to 6 to obtain a mixture, and a Lactobacillus lentus bacterial solution is added to the mixture in an amount of 3% of the mass of the mixture. The mixture is fermented at 30°C for 10 days. After the fermentation is completed, an impurity remover is added to the fermentation liquid in an amount of 5‰ of the mass of the fermentation liquid. The mixture is stirred for 10 minutes and then filtered. The filtrate is decompressed to remove the solvent and then pasteurized at 70°C for 20 minutes. Finally, the mixture is freeze-dried at -20°C for 12 hours to obtain a composite plant extract.

[0050] The preparation method of the Lactobacillus hilarii bacterial liquid comprises the following steps:

[0051] Lactobacillus lentus was inoculated into a culture medium composed of 3wt% glucose, 2.5wt% fish peptone, 1.5wt% yeast extract, 0.3wt% potassium dihydrogen phosphate, 0.2wt% calcium carbonate, and the remainder water, with an inoculation amount of 2wt%. The culture was carried out at 30°C for 36h to obtain a Lactobacillus lentus bacterial liquid.

[0052] The preparation method of the impurity remover comprises the following steps:

[0053] 1) Under an argon atmosphere, 100 g of glucose and 100 g of ammonium chloride were mixed and placed in a tube furnace, heated to 900° C. at a heating rate of 250° C. / min, then reacted at 900° C. for 3 h, cooled to room temperature to obtain a vesicle, and then the vesicle was placed in an air atmosphere and oxidized at 400° C. for 8 h to obtain a nanocarbon compound;

[0054] 2) 12.5 g of nanocarbon compound, 18 g of nanocellulose, 23 g of sodium alginate, and 12.8 g of β-glucan were added to 80 g of water, and ultrasonically treated at 60 Hz and 1800 W for 20 min, and then 2.5 g of sodium tripolyphosphate was added, and the mixture was heated to 100° C., reacted for 3 h, cooled to room temperature, and 40 g of propolis was added. The mixture was stirred and mixed at a speed of 700 rpm for 2 h at room temperature to obtain a mixed system;

[0055] 3) The mixed system obtained in step 2) is subjected to electrospinning, and a high voltage of 18KV is applied during the electrospinning process. The distance between the nozzle of the syringe and the receiving device is 12cm to obtain composite nanofibers, and then the composite nanofibers are made into a sheet structure to obtain an impurity remover.

[0056] Comparative Example 2

[0057] A method for preparing a composite plant extract comprises the following steps:

[0058] 500 g of corn silk and 500 g of buckwheat seeds were mixed, washed with water, and then sprayed with 5 g of 1.0 wt% β-cyclodextrin aqueous solution on the surface. After standing for 12 h, the mixture was freeze-dried at -20 °C for 2 h. After freeze-drying, the mixture was immediately crushed and passed through an 80-mesh sieve. Water was added at a solid-liquid ratio of 1:15 g / mL. Ultrasonic extraction was performed at room temperature, 80 Hz, and 120 W for 2 h. The mixture was then extracted for 20 min under a high-voltage pulse electric field with an electric field strength of 30 kV / cm, a pulse time of 500 μs, and a pulse frequency of 300 Hz. Then, a mixture is obtained after adjusting the pH to 6 with a 36wt% acetic acid aqueous solution, and dark golden basidiomycete liquid is added to the mixture, the dark golden basidiomycete liquid is added in an amount of 3% of the mass of the mixture, and fermented at 30°C for 10d. After the fermentation is completed, an impurity remover is added to the fermentation liquid in an amount of 5‰ of the mass of the fermentation liquid, and the mixture is stirred for 10min and filtered. The filtrate is decompressed to remove the solvent and then pasteurized at 70°C for 20min, and finally freeze-dried at -20°C for 12h to obtain a composite plant extract.

[0059] The method for preparing the dark golden basidiomycete liquid comprises the following steps:

[0060] The dark golden basidiomycete was inoculated into a culture medium prepared by uniformly mixing 8wt% skimmed milk powder, 3wt% inulin, 0.8wt% sodium dihydrogen phosphate, 0.5wt% magnesium sulfate and the remainder water, with an inoculation amount of 1.5wt%. The culture was carried out at 30°C for 30 hours to obtain a dark golden basidiomycete culture solution.

[0061] The preparation method of the impurity remover comprises the following steps:

[0062] 1) Under an argon atmosphere, 100 g of glucose and 100 g of ammonium chloride were mixed and placed in a tube furnace, heated to 900° C. at a heating rate of 250° C. / min, then reacted at 900° C. for 3 h, cooled to room temperature to obtain a vesicle, and then the vesicle was placed in an air atmosphere and oxidized at 400° C. for 8 h to obtain a nanocarbon compound;

[0063] 2) 12.5 g of nanocarbon compound, 18 g of nanocellulose, 23 g of sodium alginate, and 12.8 g of β-glucan were added to 80 g of water, and ultrasonically treated at 60 Hz and 1800 W for 20 min, and then 2.5 g of sodium tripolyphosphate was added, and the mixture was heated to 100° C., reacted for 3 h, cooled to room temperature, and 40 g of propolis was added. The mixture was stirred and mixed at a speed of 700 rpm for 2 h at room temperature to obtain a mixed system;

[0064] 3) The mixed system obtained in step 2) is subjected to electrospinning, and a high voltage of 18KV is applied during the electrospinning process. The distance between the nozzle of the syringe and the receiving device is 12cm to obtain composite nanofibers, and then the composite nanofibers are made into a sheet structure to obtain an impurity remover.

[0065] Comparative Example 3

[0066] A method for preparing a composite plant extract comprises the following steps:

[0067] 500g corn silk and 500g bitter buckwheat seeds were mixed, washed with water, and then sprayed with 5g 1.0wt% β-cyclodextrin aqueous solution on the surface. After standing for 12h, freeze-dried at -20℃ for 2h. After freeze-drying, they were immediately crushed and passed through an 80-mesh sieve. Water was added at a solid-liquid ratio of 1:15g / mL. Ultrasonic extraction was performed at room temperature, 80Hz, and 120W for 2h; then, extraction was performed under a high-voltage pulse electric field with an electric field strength of 30kV / cm, a pulse time of 500μs, and a pulse frequency of 300Hz for 20min, and then 36wt% acetic acid was used to extract the seeds. The solution was adjusted to pH 6 to obtain a mixture, and a mixed bacterial solution of Lactobacillus tarda and Basidiomycetes aureus in a mass ratio of 1:2 was added to the mixture, and the added amount of the mixed bacterial solution was 3% of the mass of the mixture. The mixture was fermented at 30°C for 10 days. After the fermentation was completed, an impurity remover was added to the fermentation liquid in an amount of 5‰ of the mass of the fermentation liquid. The mixture was stirred for 10 minutes and then filtered. The filtrate was decompressed to remove the solvent and then pasteurized at 70°C for 20 minutes. Finally, it was freeze-dried at -20°C for 12 hours to obtain a composite plant extract.

[0068] The preparation method of the Lactobacillus hilarii bacterial liquid comprises the following steps:

[0069] Lactobacillus lentus was inoculated into a culture medium composed of 3wt% glucose, 2.5wt% fish peptone, 1.5wt% yeast extract, 0.3wt% potassium dihydrogen phosphate, 0.2wt% calcium carbonate, and the remainder water, with an inoculation amount of 2wt%. The culture was carried out at 30°C for 36h to obtain a Lactobacillus lentus bacterial liquid.

[0070] The method for preparing the dark golden basidiomycete liquid comprises the following steps:

[0071] The dark golden basidiomycete was inoculated into a culture medium prepared by uniformly mixing 8wt% skimmed milk powder, 3wt% inulin, 0.8wt% sodium dihydrogen phosphate, 0.5wt% magnesium sulfate and the remainder water, with an inoculation amount of 1.5wt%. The culture was carried out at 30°C for 30 hours to obtain a dark golden basidiomycete culture solution.

[0072] The preparation method of the impurity remover comprises the following steps:

[0073] 1) 18 g of nanocellulose, 23 g of sodium alginate, and 12.8 g of β-glucan were added to 80 g of water, and ultrasonically treated at 60 Hz and 1800 W for 20 min, and then 2.5 g of sodium tripolyphosphate was added, and the mixture was heated to 100° C., reacted for 3 h, cooled to room temperature, and 40 g of propolis was added. The mixture was stirred and mixed at a speed of 700 rpm at room temperature for 2 h to obtain a mixed system;

[0074] 2) The mixed system obtained in step 1) is subjected to electrospinning, and a high voltage of 18KV is applied during the electrospinning process. The distance between the nozzle of the syringe and the receiving device is 12cm to obtain composite nanofibers, and then the composite nanofibers are made into a sheet structure to obtain an impurity remover.

[0075] Comparative Example 4

[0076] A method for preparing a composite plant extract comprises the following steps:

[0077] 500g corn silk and 500g bitter buckwheat seeds were mixed, washed with water, and then sprayed with 5g 1.0wt% β-cyclodextrin aqueous solution on the surface. After standing for 12h, freeze-dried at -20℃ for 2h. After freeze-drying, they were immediately crushed and passed through an 80-mesh sieve. Water was added at a solid-liquid ratio of 1:15g / mL. Ultrasonic extraction was performed at room temperature, 80Hz, and 120W for 2h; then, extraction was performed under a high-voltage pulse electric field with an electric field strength of 30kV / cm, a pulse time of 500μs, and a pulse frequency of 300Hz for 20min, and then 36wt% acetic acid was used to extract the seeds. The solution was adjusted to pH 6 to obtain a mixture, and a mixed bacterial solution of Lactobacillus tarda and Basidiomycetes aureus in a mass ratio of 1:2 was added to the mixture, and the added amount of the mixed bacterial solution was 3% of the mass of the mixture. The mixture was fermented at 30°C for 10 days. After the fermentation was completed, an impurity remover was added to the fermentation liquid in an amount of 5‰ of the mass of the fermentation liquid. The mixture was stirred for 10 minutes and then filtered. The filtrate was decompressed to remove the solvent and then pasteurized at 70°C for 20 minutes. Finally, it was freeze-dried at -20°C for 12 hours to obtain a composite plant extract.

[0078] The preparation method of the Lactobacillus hilarii bacterial liquid comprises the following steps:

[0079] Lactobacillus lentus was inoculated into a culture medium composed of 3wt% glucose, 2.5wt% fish peptone, 1.5wt% yeast extract, 0.3wt% potassium dihydrogen phosphate, 0.2wt% calcium carbonate, and the remainder water, with an inoculation amount of 2wt%. The culture was carried out at 30°C for 36h to obtain a Lactobacillus lentus bacterial liquid.

[0080] The method for preparing the dark golden basidiomycete liquid comprises the following steps:

[0081] The dark golden basidiomycete was inoculated into a culture medium prepared by uniformly mixing 8wt% skimmed milk powder, 3wt% inulin, 0.8wt% sodium dihydrogen phosphate, 0.5wt% magnesium sulfate and the remainder water, with an inoculation amount of 1.5wt%. The culture was carried out at 30°C for 30 hours to obtain a dark golden basidiomycete culture solution.

[0082] The preparation method of the impurity remover comprises the following steps:

[0083] 1) Under an argon atmosphere, 100 g of glucose and 100 g of ammonium chloride were mixed and placed in a tube furnace, heated to 900° C. at a heating rate of 250° C. / min, then reacted at 900° C. for 3 h, cooled to room temperature to obtain a vesicle, and then the vesicle was placed in an air atmosphere and oxidized at 400° C. for 8 h to obtain a nanocarbon compound;

[0084] 2) 12.5 g of nanocarbon compound, 18 g of nanocellulose, 23 g of sodium alginate, and 12.8 g of β-glucan were added to 80 g of water, and 2.5 g of sodium tripolyphosphate was added after ultrasonic treatment at 60 Hz and 1800 W for 20 min, and the mixture was heated to 100° C., reacted for 3 h, and cooled to room temperature to obtain a mixed system;

[0085] 3) The mixed system obtained in step 2) is subjected to electrospinning, and a high voltage of 18KV is applied during the electrospinning process. The distance between the nozzle of the syringe and the receiving device is 12cm to obtain composite nanofibers, and then the composite nanofibers are made into a sheet structure to obtain an impurity remover.

[0086] Comparative Example 5

[0087] A method for preparing a composite plant extract comprises the following steps:

[0088] 500 g corn silk and 500 g bitter buckwheat seeds were mixed, washed with water, and then sprayed with 5 g 1.0 wt% β-cyclodextrin aqueous solution on the surface. After standing for 12 h, they were freeze-dried at -20 ° C for 2 h. After freeze-drying, they were immediately crushed and passed through an 80-mesh sieve. Water was added at a solid-liquid ratio of 1:15 g / mL. Ultrasonic extraction was performed at room temperature, 80 Hz, and 120 W for 2 h. Then, extraction was performed under a high-voltage pulse electric field with an electric field strength of 30 kV / cm, a pulse time of 500 μs, and a pulse frequency of 300 Hz. The method comprises the following steps: taking the mixture for 20 minutes, adjusting the pH value to 6 with a 36wt% acetic acid aqueous solution to obtain a mixture, adding a mixed bacterial solution of a Lactobacillus lentus bacterial solution and a dark golden basidiomycete bacterial solution in a mass ratio of 1:2 to the mixture, and the addition amount of the mixed bacterial solution is 3% of the mass of the mixture, and fermenting at 30°C for 10 days. After the fermentation is completed, filtering, and evaporating the filtrate under reduced pressure to remove the solvent, and then pasteurizing at a temperature of 70°C for 20 minutes, and finally freeze-drying at -20°C for 12 hours to obtain a composite plant extract.

[0089] The preparation method of the Lactobacillus hilarii bacterial liquid comprises the following steps:

[0090] Lactobacillus lentus was inoculated into a culture medium composed of 3wt% glucose, 2.5wt% fish peptone, 1.5wt% yeast extract, 0.3wt% potassium dihydrogen phosphate, 0.2wt% calcium carbonate, and the remainder water, with an inoculation amount of 2wt%. The culture was carried out at 30°C for 36h to obtain a Lactobacillus lentus bacterial liquid.

[0091] The method for preparing the dark golden basidiomycete liquid comprises the following steps:

[0092] The dark golden basidiomycete was inoculated into a culture medium prepared by uniformly mixing 8wt% skimmed milk powder, 3wt% inulin, 0.8wt% sodium dihydrogen phosphate, 0.5wt% magnesium sulfate and the remainder water, with an inoculation amount of 1.5wt%. The culture was carried out at 30°C for 30 hours to obtain a dark golden basidiomycete culture solution.

[0093] Test Example 1

[0094] 50g of fresh roses were dried at 60°C for 8h, mixed with 30g of the composite plant extracts prepared in Example 1 and Comparative Examples 1-5, and 10g of Lapsang Souchong black tea, ground into powder, and 5g of the powder was brewed with 250mL of 100°C water for 10min to obtain rose tea, and the light transmittance test was performed:

[0095] Transmittance test: Using spectrophotometry, take 10 mL of the above rose tea in a cuvette, use distilled water as a reference solution, and measure the transmittance of the rose tea at 720 nm, expressed as T (%); the test results are shown in Table 1:

[0096] Table 1 Transmittance test results

[0097] T(％) Example 1 93 Comparative Example 1 91.8 Comparative Example 2 90.2 Comparative Example 3 80.3 Comparative Example 4 85.1 Comparative Example 5 50.6

[0098] It can be seen from the experimental results in Table 1 that the rose tea containing the composite plant extract prepared in Example 1 has the best light transmittance. The possible reason for this phenomenon is that the porous structure of the nano-carbon compound can adsorb heavy metals and miscellaneous bacteria in the plant extract, sodium alginate and β-glucan can promote propolis to form a composite film attached to the surface of the material formed by the nano-carbon compound, nano-cellulose, etc., and the active groups on the surface of the propolis can purify the composite plant extract system and react with the bitter peroxidized polyphenols in the composite plant extract to effectively remove the peroxidized polyphenols and unstable pigment substances in the extract, so that the prepared rose tea has better gloss.

[0099] Test Example 2

[0100] Blood pressure lowering effect test: The rose tea containing the composite plant extract prepared in Example 1 and Comparative Examples 1-5 of the present invention obtained in Test Example 1 was tested for blood pressure lowering effect. The test subjects were SHR male rats, SPF grade, weighing 300-400g / rat, and the control group selected ordinary male Wistar rats, SPF grade, weighing 300-400g / rat. SHR rats and Wistar rats were provided by the Shanghai Experimental Animal Center of the Chinese Academy of Sciences. Randomly take 15 Wistar rats as the normal group; randomly take 105 SHR rats with hypertension and divide them into 6 experimental groups and 1 model group, each group of 15 rats, divided into normal group, model group, experimental group, normal group, model group, experimental group, normal group, model group, experimental group are given basic feed and free drinking water every day, and the breeding environment is 25℃. The experimental group is additionally fed with rose tea containing composite plant extracts in Example 1 and Comparative Examples 1-5 every day, with a dose of 8mL / 10g body weight, and fed continuously for 5 days a week, stopped for 2 days, treated for 4 weeks, and the blood pressure (systolic pressure) of each group of rats is tested every week. The rat blood pressure test was performed using a BP-2010A animal non-invasive sphygmomanometer (provided by Shenzhen Ruiwode Life Science Co., Ltd.). The results were calculated by taking the average value of multiple values. The blood pressure measurement results of SHR rats are shown in Table 2. The test results are statistically significant.

[0101] Table 2 Blood pressure lowering effect test results

[0102]

[0103] It can be seen from the test results of the antihypertensive effect in Table 2 that the rose tea prepared by the present invention can effectively control blood pressure, and after the rose tea to which the composite plant extract prepared in Example 1 was added was fed for 4 weeks, the blood pressure of SHR rats gradually recovered and approached the normal value. The possible reason is that Lactobacillus lentus and dark golden basidiomycete can promote the conversion of glutamate in corn silk and buckwheat into γ-aminobutyric acid, which is an important inhibitory neurotransmitter in the central nervous system, has the effects of anticonvulsant, promoting brain metabolism, preventing cerebral arteriosclerosis and significantly inhibiting the increase of blood pressure, thereby improving the antihypertensive effect of the composite plant extract, and by adding an impurity remover, a large amount of impurities and pigments are removed, so that the content of γ-aminobutyric acid is higher, further improving its antihypertensive effect.

Claims

1. A method for preparing a composite plant extract, It is characterized in that The method comprises the following steps: mixing corn silk and bitter buckwheat seeds, washing them with water, spraying the surfaces with a 0.5-1.5wt% β-cyclodextrin aqueous solution, standing them for 10-12 hours, and then freeze-drying them at -30 to -20°C for 1-2 hours; immediately crushing and passing through an 80-100 mesh sieve after freeze-drying, adding water at a material-liquid ratio of 1:10-15g / mL, and ultrasonically extracting them at room temperature, 60-100Hz, and 120-150W for 1-2 hours; and then extracting them under a high-voltage pulse electric field for 1 hour. 0-30min, then adjusting the pH to 5-7 with 30-36wt% acetic acid aqueous solution to obtain a mixture, adding the mixed bacterial solution to the mixture, fermenting at 25-30°C for 8-10d, after the fermentation is completed, adding an impurity remover to the fermentation liquid, stirring for 10-20min, filtering, decompressing and distilling the filtrate to remove the solvent, pasteurizing at a temperature of 60-80°C for 20-30min, and finally freeze-drying at -30~-20°C for 6~12h to obtain a composite plant extract; The mixed bacterial liquid is a mixture of Lactobacillus lentus bacterial liquid and dark golden basidiomycete bacterial liquid in a mass ratio of 1-2:2-3; The preparation method of the impurity remover comprises the following steps: 1) Under an argon atmosphere, 100-120 g of glucose and 100-120 g of ammonium chloride are mixed and placed in a tube furnace, and the mixture is heated to 800-900° C. at a heating rate of 200-250° C. / min, and then reacted at 800-900° C. for 3-5 hours, cooled to room temperature, and a foam is obtained, and then the foam is placed in an air atmosphere and oxidized at 300-400° C. for 6-8 hours to obtain a nanocarbon compound; 2) Add 10-15g of nanocarbon compound, 15-20g of nanocellulose, 20-25g of sodium alginate and 10-15g of β-glucan to 50-80g of water, add 1-3g of sodium tripolyphosphate after ultrasonic treatment at 50-80Hz and 1500-2000W for 20-30min, heat to 80-100℃, react for 2-4h, cool to room temperature, add 30-50g of propolis, stir and mix at 600-800rpm for 1-2h at room temperature to obtain a mixed system; 3) The mixed system obtained in step 2) is subjected to electrospinning, and a high voltage of 15-20 kV is applied during the electrospinning process. The distance between the nozzle of the syringe and the receiving device is 10-15 cm to obtain composite nanofibers, and then the composite nanofibers are made into a sheet structure to obtain an impurity remover.

2. The method for preparing the composite plant extract according to claim 1, Features: The dosage ratio of the corn silk, the tartary buckwheat seeds and the beta-cyclodextrin aqueous solution is 500-1000:500-1000:5-10.

3. The method for preparing the composite plant extract according to claim 1, Features: The high-voltage pulse electric field extraction conditions are as follows: the electric field strength is 20-40 kV / cm, the pulse time is 400-500 μs, and the pulse frequency is 200-300 Hz.

4. The method for preparing the composite plant extract according to claim 1, It is characterized in that The preparation method of the Lactobacillus hilarii bacterial liquid comprises the following steps: The method comprises inoculating Lactobacillus lentus to a culture medium prepared by mixing 2-5wt% glucose, 2-3wt% fish peptone, 1-2wt% yeast extract, 0.1-0.5wt% potassium dihydrogen phosphate, 0.1-0.5wt% calcium carbonate and the balance water, with an inoculation amount of 1-3wt%, and culturing the culture medium at a temperature of 25-35°C for 24-36h to obtain a Lactobacillus lentus bacterial liquid.

5. The method for preparing the composite plant extract according to claim 1, It is characterized in that The method for preparing the dark golden basidiomycete liquid comprises the following steps: The dark golden basidiomycete is inoculated into a culture medium prepared by uniformly mixing 6-10wt% skimmed milk powder, 2-5wt% inulin, 0.5-1.2wt% sodium dihydrogen phosphate, 0.1-1.0wt% magnesium sulfate and the balance water, with an inoculation amount of 1-2wt%. The culture is carried out at a temperature of 25-35°C for 24-36h to obtain a dark golden basidiomycete bacterial liquid.

6. The method for preparing the composite plant extract according to claim 1, Features: The amount of the impurity remover added is 1-3‰ of the fermentation liquid mass.

7. A composite plant extract, It is characterized in that The compound plant extract is prepared by the preparation method of any one of claims 1 to 6.

8. Use of the composite plant extract according to claim 7 in preparing heat-clearing rose tea, It is characterized in that The specific application is as follows: fresh roses and plants with heat-clearing function are dried, mixed with the composite plant extract according to claim 7 and black tea, ground into powder and packaged.

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