Phytocide cosmetic composition and its preparation method

Abstract

The present invention relates to a phytobactericide cosmetic composition and a preparation method thereof. By mixing extracts of Chamaecyparis obtusa leaves, Platycladus orientalis extracts, Cupressus sempervirens L. var. horizontalis extracts, Cryptomeria fortunei Hooibrenk ex Otto et Dietr. extracts, Sabina chinensis (L.) Ant. xylem extracts, Artemisia capillaris Thunb. extracts, Dendranthema zawadskii (Herb.) Tzvelev extracts, Angelica acutiloba (Sieb.) Kitag. root extracts, and Pinus palustris Mill. leaf extracts, the phytobactericide cosmetic composition has skin moisturizing effects, skin antioxidant effects, and skin elasticity improvement effects.

Description

Technical Field

[0001] The present invention relates to a phytobactericide cosmetic composition and a method for preparing the same, and more particularly, to a phytobactericide cosmetic composition having skin moisturizing effect, skin antioxidant effect and skin elasticity improving effect by mixing extracts of Chamaecyparis obtusa leaves, Platycladus orientalis extracts, Cupressus sempervirens L. var. horizontalis extracts, Cryptomeria fortunei Hooibrenk ex Otto et Dietr. extracts, Juniperus chinensis L. xylem extracts, Artemisia capillaris Thunb. extracts, Dendranthema zawadskii (Herb.) Tzvelev extracts, Angelica acutiloba Kitag. root extracts and Pinus palustris Mill. extracts, and a method for preparing the same. Background Art

[0002] The skin is the body part directly exposed to the external environment. It not only protects the important organs of our body as a protective film, but also controls water evaporation and protects the body from external infections. However, no matter how the skin blocks the invasion of viruses from the outside, external pressures such as excessive ultraviolet rays and polluted environment can cause skin irritation and ultimately lead to skin aging.

[0003] Antioxidants are added to lipid systems to prevent the formation of various unwanted compounds due to oxidation. It is known that various oxidation products generated by oxidation damage DNA or cause cancer, and thus are related to human aging. Generally, butylated hydroxy anisole (BHA) and butylated hydroxy toluene (BHT), which are widely used as phenolic synthetic antioxidants, are widely used due to their effectiveness, economy and safety. However, in addition to generally avoiding the use of synthetic additives, it is well known that excessive intake can cause serious toxic effects on the gastrointestinal mucosa, lungs, kidneys and circulatory system, etc. Therefore, it is necessary to develop safe alternative antioxidants. Therefore, the research on natural antioxidants that are harmless to the human body and have excellent antioxidant ability has a long history, and most of the natural antioxidants reported currently come from plants. The compounds with antioxidant ability in most plants are mainly polyphenol substances. Some natural antioxidants have secondary activity of forming metal complexes, but their main function is primary antioxidant activity (free radical scavenger). Therefore, natural antioxidants can be developed by exploring the free radical scavenging function of plant extracts.

[0004] This skin is an organ that exists on the outermost layer of the human body and has the function of protecting the human body. It has the functions of preventing the loss of body moisture and electrolytes and preventing the invasion of foreign substances from the outside. The skin is divided into the epidermis, dermis, and subcutaneous fat layer. The epidermis is composed of keratinocytes. The outermost stratum corneum of the epidermis plays a very important role in the penetration of substances. The epidermis consists of the basal layer, spinous layer, granular layer, and stratum corneum. The stratum corneum is composed of keratinocytes and intercellular lipids. The stratum corneum prevents the loss of moisture to the outside and requires appropriate moisture to perform its due functions. The skin barrier located in the stratum corneum is composed of ceramides, fatty acids, and cholesterol, and has the functions of preventing the invasion of foreign substances from the outside and preventing transepidermal water loss (TEWL). In addition, it is known that natural moisturizing factor (NMF) exists in the stratum corneum and plays an important role in skin moisturization.

[0005] On the other hand, with the growth of the population, urbanization, industrialization, and the increase in the number of automobiles, air pollution has intensified, and the types of pollutants have also become diversified. Particulate matter is classified into particulate matter with a diameter of less than 10 μm (PM10) and ultrafine particulate matter with a diameter of less than 2.5 μm (PM2.5) according to the particle size. The Ministry of Environment of Korea lists PM10 as an air pollutant. It is well known that such air pollutants as particulate matter can cause respiratory diseases and heart diseases. At the same time, people's attention to their impact on the skin, which occupies most of the body area, is gradually expanding.

[0006] Due to the above-mentioned problems, it is necessary to develop cosmetic compositions with skin moisturizing effects, skin antioxidant effects, and skin elasticity improvement effects, etc. using various natural extracts. However, there is currently no cosmetic composition with appropriate effects. Therefore, through the combination of various natural ingredients, unremitting research is being carried out to find cosmetic compositions with skin moisturizing effects, skin antioxidant effects, and skin elasticity improvement effects.

[0007] Prior art documents

[0008] Patent documents

[0009] Patent Document 0001: Korean Patent Publication No. 10-1777622 (announced on September 13, 2017), "Cosmetic Composition Containing Chamaecyparis obtusa Essential Oil and Cosmetics Using the Same" Summary of the invention

[0010] Technical problems to be solved

[0011] To solve the problems described above, the present invention provides a phytobacteriocin cosmetic composition and a method for preparing the same. By mixing extracts of Chamaecyparis obtusa leaves, Platycladus orientalis extracts, Cupressus sempervirens L. var. horizontalis extracts, Cryptomeria japonica D. Don extracts, Sabina chinensis (L.) Ant. xylem extracts, Artemisia capillaris Thunb. extracts, Dendranthema zawadskii (Herb.) Tzvelev extracts, Angelica acutiloba (Sieb.) Kitag. root extracts, and Pinus palustris Mill. leaf extracts, the phytobacteriocin cosmetic composition has skin moisturizing effects, skin antioxidant effects, and skin elasticity improvement effects.

[0012] Technical solutions for solving the problems

[0013] To achieve the above object, the present invention provides a phytobacteriocin cosmetic composition comprising a phytobacteriocin extract obtained by mixing extracts of Chamaecyparis obtusa leaves, Platycladus orientalis extracts, Cupressus sempervirens L. var. horizontalis extracts, Cryptomeria japonica D. Don extracts, Sabina chinensis (L.) Ant. xylem extracts, Artemisia capillaris Thunb. extracts, Dendranthema zawadskii (Herb.) Tzvelev extracts, Angelica acutiloba (Sieb.) Kitag. root extracts, and Pinus palustris Mill. leaf extracts.

[0014] At this time, the above extracts of Chamaecyparis obtusa leaves, Platycladus orientalis extracts, Cupressus sempervirens L. var. horizontalis extracts, Cryptomeria japonica D. Don extracts, Sabina chinensis (L.) Ant. xylem extracts, Artemisia capillaris Thunb. extracts, Dendranthema zawadskii (Herb.) Tzvelev extracts, Angelica acutiloba (Sieb.) Kitag. root extracts, and Pinus palustris Mill. leaf extracts are extracted using an extraction solvent selected from the group consisting of water, anhydrous or hydrous lower alcohols having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate, and 1,3-butanediol.

[0015] Moreover, relative to the total weight of the cosmetic composition, it contains 0.01% by weight to 20.0% by weight of the above phytobacteriocin extract.

[0016] Furthermore, the above phytobacteriocin extract has skin moisturizing effects, skin antioxidant effects, and skin elasticity improvement effects.

[0017] Moreover, the present invention provides a method for preparing a phytobactericide cosmetic composition, comprising: a cleaning and drying step (S10) of cleaning and drying Chamaecyparis obtusa leaves, Platycladus orientalis, Cupressus sempervirens leaves, Cryptomeria japonica leaves, Sabina chinensis xylem, Artemisia capillaris, Dendranthema zawadskii, Angelica acutiloba roots, and Pinus palustris leaves; an extraction step (S20) of extracting the Chamaecyparis obtusa leaves, Platycladus orientalis, Cupressus sempervirens leaves, Cryptomeria japonica leaves, Sabina chinensis xylem, Artemisia capillaris, Dendranthema zawadskii, Angelica acutiloba roots, and Pinus palustris leaves that have been cleaned and dried in the above cleaning and drying step (S10) using an extraction solvent; a powder preparation step (S30) of making the Chamaecyparis obtusa leaf extract, Platycladus orientalis extract, Cupressus sempervirens leaf extract, Cryptomeria japonica leaf extract, Sabina chinensis xylem extract, Artemisia capillaris extract, Dendranthema zawadskii extract, Angelica acutiloba root extract, and Pinus palustris leaf extract extracted in the above extraction step (S20) into a powder form; and a mixing step (S40) of mixing the Chamaecyparis obtusa leaf extract, Platycladus orientalis extract, Cupressus sempervirens leaf extract, Cryptomeria japonica leaf extract, Sabina chinensis xylem extract, Artemisia capillaris extract, Dendranthema zawadskii extract, Angelica acutiloba root extract, and Pinus palustris leaf extract that have been made into a powder form in the above powder preparation step (S30).

[0018] At this time, the extraction solvent in the above extraction step (S20) is an extraction solvent selected from the group consisting of water, anhydrous or hydrous lower alcohols having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate, and 1,3 - butanediol.

[0019] Moreover, the Chamaecyparis obtusa leaves, Platycladus orientalis, Cupressus sempervirens leaves, Cryptomeria japonica leaves, Sabina chinensis xylem, Artemisia capillaris, Dendranthema zawadskii, Angelica acutiloba roots, and Pinus palustris leaves that have been cleaned and dried in the above extraction step (S20) are reflux - extracted 2 to 4 times at a temperature of 60 to 90 °C for 3 to 5 hours each time, and then cooled to room temperature.

[0020] Moreover, the Chamaecyparis obtusa leaf extract, Platycladus orientalis extract, Cupressus sempervirens leaf extract, Cryptomeria japonica leaf extract, Sabina chinensis xylem extract, Artemisia capillaris extract, Dendranthema zawadskii extract, Angelica acutiloba root extract, and Pinus palustris leaf extract extracted in the above powder preparation step (S30) are filtered with filter paper, and then concentrated under reduced pressure and freeze - dried at a temperature below 50 °C to make a powder form.

[0021] Moreover, the Chamaecyparis obtusa leaf extract, Platycladus orientalis extract, Cupressus sempervirens leaf extract, Cryptomeria japonica leaf extract, Sabina chinensis xylem extract, Artemisia capillaris extract, Dendranthema zawadskii extract, Angelica acutiloba root extract, and Pinus palustris leaf extract that have been made into a powder form in the above mixing step (S40) are mixed at a weight ratio of 0.1 to 10.0:0.1 to 10.0:0.1 to 10.0:0.1 to 10.0:0.1 to 10.0:0.1 to 10.0:0.1 to 10.0:0.1 to 10.0:0.1 to 10.0.

[0022] Advantages of the Invention

[0023] The phytobactericidal cosmetic composition prepared according to the preparation method of the present invention is mixed with extracts of Chamaecyparis obtusa leaves, Platycladus orientalis extracts, Cupressus sempervirens leaves extracts, Cryptomeria japonica leaves extracts, Sabina chinensis xylem extracts, Artemisia capillaris extracts, Chrysanthemum zawadskii extracts, Angelica acutiloba root extracts, and Pinus palustris leaf extracts, and thus has skin moisturizing effect, skin antioxidant effect, and skin elasticity improvement effect. Description of the Drawings

[0024] Figure 1 It is a flowchart showing the preparation method of the cosmetic composition of the present invention.

[0025] Figure 2 It is a bar graph showing the cell survival rates at different treatment concentrations of Example 1 and Comparative Examples 1 to 9.

[0026] Figure 3 It is a bar graph showing the free radical scavenging effects at different treatment concentrations of Example 1 and Comparative Examples 1 to 9.

[0027] Figure 4 It is a bar graph showing the elastase inhibitory abilities at different treatment concentrations of Example 1 and Comparative Examples 1 to 9.

[0028] Figure 5 It is a bar graph showing the collagenase inhibitory abilities at different treatment concentrations of Example 1 and Comparative Examples 1 to 9

[0029] Figure 6 It is a bar graph showing the skin moisturizing improvement effects of Example 1 and Comparative Examples 1 to 9.

[0030] Figure 7 It is a bar graph showing the transdermal water loss amounts of Example 1 and Comparative Examples 1 to 9.

[0031] Explanation of Reference Numerals

[0032] (S10): Cleaning and Drying Step

[0033] (S20): Extraction Step

[0034] (S30): Powder Preparation Step

[0035] (S40): Mixing Step Detailed Description of the Invention

[0036] The following detailed description of the present invention is for embodiments that can implement the present invention and refers to the accompanying drawings shown as examples of the embodiments. The detailed description of these embodiments enables those skilled in the art to fully implement the present invention. It should be understood that the various embodiments of the present invention are different but not necessarily mutually exclusive. For example, without departing from the spirit and scope of the present invention, the specific shapes, structures, and characteristics described herein in relation to one embodiment can be implemented in another embodiment. And, it should be understood that without departing from the spirit and scope of the present invention, the positions or arrangements of the respective structural elements in each described embodiment can be changed.

[0037] Therefore, the detailed description set forth below should not be construed in a limiting sense, and the scope of the present invention is defined only by the appended claims of invention and all scopes of all equivalents claimed by these claims of invention if appropriately described. Similar reference numerals in the drawings denote the same or similar functions in all aspects.

[0038] When considering the functions of the terms used in the present invention, the terms are selected as generally used currently as widely as possible, but these may change according to the intentions or precedents of those skilled in the art, the emergence of new technologies, etc. And, in specific cases, there are also terms arbitrarily selected by the applicant, and the meanings in such cases will be described in detail in the part of the invention description. Therefore, the terms used in the present invention should be defined according to the meanings of the terms and the overall content of the present invention, rather than just the names of the terms.

[0039] In the present invention, when a component "comprises" a certain structural element, unless otherwise specified, it means that it may also include other structural elements without excluding other structural elements.

[0040] Hereinafter, the phytoalexin cosmetic composition of the present invention and its preparation method will be described in more detail.

[0041] The phytoalexin cosmetic composition of the present invention comprises a phytoalexin extract obtained by mixing extracts of Chamaecyparis obtusa leaves, Platycladus orientalis extracts, Cupressus sempervirens leaves extracts, Cryptomeria japonica leaves extracts, Sabina chinensis xylem extracts, Artemisia capillaris extracts, Dendranthema zawadskii extracts, Angelica acutiloba root extracts, and Pinus palustris leaf extracts.

[0042] The above-mentioned hinoki leaf extract refers to the extract obtained from the leaves of the hinoki tree. The above-mentioned hinoki tree (Chamaecyparis obtusa (Siebold & Zucc.) Endl.) is an evergreen tree of the Cupressaceae family in the order Coniferales of gymnosperms. Due to its excellent antibacterial and bactericidal effects, it is widely used as a health care product material. It is used as a first-class interior decoration material in Japan and is also known as "hinoki". The hinoki tree contains a large amount of natural antibacterial substances called phytocides, so it has excellent bactericidal effects. And because of its strong water resistance, when it comes into contact with water, its unique fragrance will spread, playing a role in eliminating odors. Therefore, it is often used as a material for cutting boards recently.

[0043] The above-mentioned oriental arborvitae extract refers to the extract obtained from the oriental arborvitae. At this time, the used part of the oriental arborvitae is not restricted, and its whole herb can be used. The above-mentioned oriental arborvitae (Platycladus orientalis) is an evergreen tree of the Cupressaceae family in the order Coniferales of the sub-class Coniferopsida of gymnosperms, up to 25 m tall and 1 m in diameter, but is a shrub with small branches spreading vertically. The scaly leaves are pointed, alternate, and there are branches in the middle. There is almost no difference between the front and back of the leaves, and there are several white dots. Because of its beautiful tree shape, it is planted as a hedge and for ornamental purposes.

[0044] The above-mentioned Mediterranean cypress wood extract refers to the extract obtained from the xylem of the Mediterranean cypress. The above-mentioned Mediterranean cypress (Cupressus sempervirens) is a cypress native to the eastern Mediterranean region. It is an evergreen coniferous tree that can reach a height of about 40 - 45 m and grows well in warm and dry places. It has antiseptic and astringent properties and is effective against acne, greasiness, excessive dampness, and excessive sweating.

[0045] The above-mentioned Japanese cedar leaf extract refers to the extract obtained from the leaves of the Japanese cedar. The above-mentioned Japanese cedar (Cryptomeria japonica) is an evergreen tree of the Taxodiaceae family in the order Coniferales of gymnosperms, and grows well in valleys with an average annual temperature of 12 - 14 °C and a rainfall of more than 3000 mm. It is an endemic species in Japan. It reaches a height of 40 m and a diameter of 1 - 2 m. The bark is reddish-brown, longitudinally cracked, the branches and leaves are dense, and the tree shape is conical. The leaves are curved into needle shapes and arranged in a spiral pattern, and do not fall off even when dried.

[0046] The above-mentioned Chinese juniper xylem extract refers to the extract obtained from the xylem of the Chinese juniper. The above-mentioned Chinese juniper (Juniperus chinensis) is an evergreen tree of the Cupressaceae family in the order Coniferales of the sub-class Coniferopsida of gymnosperms, and can reach a height of about 20 m. The newly sprouted branches are green, the 3-year-old branches are dark brown, and the 7 - 8-year-old branches have scaly soft leaves, but the leaves are pointed needles when germinating. The leaves face each other or turn around and grow densely, and the branches cannot be seen.

[0047] The above-mentioned Artemisia capillaris Thunb. extract refers to the extract obtained from Artemisia capillaris Thunb. At this time, the used part of Artemisia capillaris Thunb. is not restricted, and its whole herb can be used. The above-mentioned Artemisia capillaris Thunb. is a perennial herbaceous plant of the Compositae family in the Campanulales order of dicotyledonous plants, and it often grows on sandy land by streams. It is 30-100 cm tall. The lower part is woody, like a tree, and many branches are split. The leaves are clustered at the ends of the non-flowering branches, and the petioles are long. The lower leaves have long petioles, 1.5-9 cm long and 1-7 cm wide, and are divided into twice-pinnate. The bifurcated segments are as thin as threads, about 0.3 mm wide, and are covered with filamentous hairs all over. The leaves become smaller towards the top.

[0048] The above-mentioned Dendranthema zawadskii (Herb.) Tzvelev extract refers to the extract obtained from Dendranthema zawadskii (Herb.) Tzvelev. At this time, the used part of Dendranthema zawadskii (Herb.) Tzvelev is not restricted, and its whole herb can be used. The above-mentioned Dendranthema zawadskii (Herb.) Tzvelev is a perennial herbaceous plant of the Compositae family in the Campanulales order of dicotyledonous plants, and it grows to a height of 10-60 cm in alpine meadows. The rhizome grows laterally and produces prostrate hairs. The leaves are alternate, the lower leaves have long petioles, are ovate, 1-3.5 cm long and 1-4 cm wide. It is split twice like a swan's feather or completely like a swan's feather, and the lobes are 1-2 mm wide. It is similar to Polygonum chinense L., but the leaves are divided very narrowly. It is for ornamental use, and the whole plant is used to treat symptoms such as qi and blood deficiency in women, stomach problems, and asthma. It is distributed in South Korea, Japan, northeastern China, and Siberia. It is found on Jeju Island, with fleshy and finely divided leaves and white flowers, and is called Hanra Polygonum chinense L. (var. coreanum).

[0049] The above-mentioned Angelica tenuissima root extract is the extract obtained from the roots of Angelica tenuissima. The above-mentioned Angelica tenuissima is a perennial herbaceous plant of the Apiaceae family in the Apiales order of dicotyledonous plants, and it grows at the foot of deep mountains. It is 30-80 cm tall. The whole herb is hairless and has a fragrance. The stem is erect and branched. The leaves are alternate, the leaves attached to the roots have long petioles, and the leaves attached to the stems have leaf sheaths. It is divided into a triply pinnate compound leaf, and the lobes are linear. In traditional Chinese medicine, the roots are dug out and dried in autumn, called Angelica tenuissima, and are used to treat headache, joint pain, toothache, abdominal pain, diarrhea, and eczema. It is widely distributed in South Korea (Gyeongsangnam-do, Gyeongsangbuk-do, Chungcheongnam-do, Chungcheongbuk-do, Gangwon-do, Pyeongannam-do).

[0050] The above Pinus Palustris Leaf Extract refers to an extract obtained from the leaves of Pinus Palustris native to North America. Pinus Palustris is an evergreen coniferous tree native to North America, with a height of about 30 - 36 m, three small leaves, soft and slender leaves, and a unique tree shape. The above Pinus Palustris is a tree that can be planted in the central region of Korea, with strong cold resistance, pollution resistance, and vitality against pests and diseases. It contains chlorophyll components as green chlorophyll, which helps to excrete toxins, maintain skin health, condition the skin, and help maintain vitality and vigor.

[0051] Preferably, the above Chamaecyparis obtusa leaf extract, Platycladus orientalis extract, Cupressus sempervirens leaf extract, Cryptomeria japonica leaf extract, Sabina chinensis xylem extract, Artemisia capillaris extract, Dendranthema zawadskii extract, Angelica acutiloba root extract, and Pinus Palustris leaf extract are extracted using an extraction solvent selected from the group consisting of water, anhydrous or hydrous lower alcohols having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate, and 1,3 - butanediol.

[0052] Moreover, in the above phytobacteriocin extract, preferably, the Chamaecyparis obtusa leaf extract, Platycladus orientalis extract, Cupressus sempervirens leaf extract, Cryptomeria japonica leaf extract, Sabina chinensis xylem extract, Artemisia capillaris extract, Dendranthema zawadskii extract, Angelica acutiloba root extract, and Pinus Palustris leaf extract are mixed in a weight ratio of 0.1 - 10.0:0.1 - 10.0:0.1 - 10.0:0.1 - 10.0:0.1 - 10.0:0.1 - 10.0:0.1 - 10.0:0.1 - 10.0:0.1 - 10.0, and more specifically, preferably mixed in a weight ratio of 1:1:1:1:1:1:1:1:1.

[0053] Moreover, preferably, relative to the total weight of the cosmetic composition, it contains 0.01 wt% to 20.0 wt% of the above phytobacteriocin extract.

[0054] For example, the phytobacteriocin cosmetic composition of the present invention may contain 2 wt% of Chamaecyparis obtusa extraction powder, 2 wt% of Platycladus orientalis extraction powder, 2 wt% of Cupressus sempervirens leaf extraction powder, 2 wt% of Cryptomeria japonica leaf extraction powder, 2 wt% of Sabina chinensis xylem extraction powder, 2 wt% of Artemisia capillaris extraction powder, 2 wt% of Dendranthema zawadskii extraction powder, 2 wt% of Angelica acutiloba root extraction powder, and 2 wt% of Pinus Palustris leaf extraction powder, and the total composition may also contain 18 wt% as the sum of these.

[0055] Moreover, the above phytobacteriocin extract may have skin moisturizing effect, skin antioxidant effect, and skin elasticity improving effect.

[0056] In addition to the composition of the present invention, the present invention may further comprise ingredients commonly used in cosmetic compositions, such as antioxidants, stabilizers, solubilizers, vitamins, pigments, dyes, fragrances and other conventional excipients, as well as carriers.

[0057] The cosmetic composition of the present invention can be prepared into any dosage form commonly prepared in the art. For example, it can be formulated into solutions, suspensions, emulsions, pastes, gels, skin creams, skin lotions, powders, soaps, surfactant-containing cleansers, oils, foundations, emulsion foundations, wax foundations and sprays, etc., but not limited thereto. More specifically, it can be prepared into the dosage forms of skin softeners, nutrient solutions, nutrient creams, massage creams, essence, facial masks, eye creams, cleansing creams, cleansing foams, cleansing waters, facial masks, sprays or powders.

[0058] When the dosage form of the present invention is a paste, cream or gel, one or more of animal oils, vegetable oils, waxes, paraffins, starches, tragacanth, cellulose derivatives, polyethylene glycols, silicones, bentonites, silica, talc or zinc oxide can be selected as the carrier component.

[0059] When the dosage form of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder can be used as the carrier component. In particular, when the dosage form of the present invention is a spray, propellants such as chlorofluorocarbons, propane / butane or dimethyl ether can also be included.

[0060] When the dosage form of the present invention is a solution or an emulsion, solvents, solubilizers or emulsifiers are used as the carrier components. For example, one or more of water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, butylene glycol, 1,3-butylene glycol, polyoxyethylene hydrogenated castor oil, glycerol, glycerin, aliphatic esters, phenoxyethanol, triethanolamine, polyethylene glycol, beeswax, polysorbate 60, sorbitan sesquioleate, paraffin, sorbitan stearate, lipophilic monoglyceride stearate, stearic acid, glyceryl stearate / PEG-400 stearate, carboxyl polymers, sitosterol, polyglycerol 2-oleate, ceramides, cholesterol, steareth-4, dicetyl phosphate, macadamia nut oil, carbomer, xanthan gum or fatty acid esters of sorbitol can be selected for use.

[0061] When the dosage form of the present invention is a suspension, one or more of a liquid diluent, a suspending agent, microcrystalline cellulose, hydroxyethyl cellulose, sodium hyaluronate, phenoxyethanol, aluminum metahydroxide, bentonite, stearic acid, cetyl alcohol, glyceryl monostearate, polyoxyethylene sorbitan monostearate, sorbitan sesquioleate, glyceryl monostearate / glyceryl stearate / polyoxyethylene stearate, wax, paraffin, squalane, caprylic / capric triglyceride, carbomer, triethanolamine, agar or tragacanth can be selected as carrier components. The above-mentioned liquid diluent is, for example, water, ethanol, glycerol, butanediol or propylene glycol, and the above-mentioned suspending agent is, for example, ethoxylated isostearyl alcohol, polyoxyethylene sorbitan ester and polyoxyethylene sorbitan anhydride ester.

[0062] When the dosage form of the present invention is a surfactant-containing cleaner, one or more of fatty alcohol sulfates, fatty alcohol ether sulfates, sulfosuccinic acid monoesters, hydroxyethanesulfonates, imidazoline derivatives, methyl taurine, sarcosinates, fatty acid amide ether sulfates, alkyl amino betaines, fatty alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, wool fat derivatives or ethoxylated glycerol fatty acid esters can be selected as carrier components.

[0063] Moreover, the usage efficacy of the cosmetic composition of an embodiment of the present invention can be appropriately adjusted according to an individual's skin condition, state and external environmental conditions.

[0064] On the other hand, the present invention provides a method for preparing a phytocidal cosmetic composition using extracts of Chamaecyparis obtusa leaves, Platycladus orientalis extracts, Cupressus sempervirens L. var. horizontalis extracts, Cryptomeria japonica D. Don extracts, Sabina chinensis (L.) Ant. extracts, Artemisia capillaris Thunb. extracts, Dendranthema zawadskii (Herb.) Tzvelev extracts, Angelica acutiloba Kitag. extracts and Pinus palustris Mill. extracts.

[0065] The preparation method of the phytobactericide cosmetic composition of the present invention comprises: a cleaning and drying step (S10) of cleaning and drying Chamaecyparis obtusa leaves, Platycladus orientalis, Cupressus sempervirens leaves, Cryptomeria japonica leaves, Sabina chinensis xylem, Artemisia capillaris, Dendranthema zawadskii, Angelica acutiloba roots, and Pinus palustris leaves; an extraction step (S20) of extracting the Chamaecyparis obtusa leaves, Platycladus orientalis, Cupressus sempervirens leaves, Cryptomeria japonica leaves, Sabina chinensis xylem, Artemisia capillaris, Dendranthema zawadskii, Angelica acutiloba roots, and Pinus palustris leaves that have been cleaned and dried in the above cleaning and drying step (S10) using an extraction solvent; a powder preparation step (S30) of making the Chamaecyparis obtusa leaf extract, Platycladus orientalis extract, Cupressus sempervirens leaf extract, Cryptomeria japonica leaf extract, Sabina chinensis xylem extract, Artemisia capillaris extract, Dendranthema zawadskii extract, Angelica acutiloba root extract, and Pinus palustris leaf extract extracted in the above extraction step (S20) into powder form; and a mixing step (S40) of mixing the Chamaecyparis obtusa leaf extract, Platycladus orientalis extract, Cupressus sempervirens leaf extract, Cryptomeria japonica leaf extract, Sabina chinensis xylem extract, Artemisia capillaris extract, Dendranthema zawadskii extract, Angelica acutiloba root extract, and Pinus palustris leaf extract that have been made into powder form in the above powder preparation step (S30).

[0066] First, the cleaning and drying step is carried out.

[0067] In the above cleaning and drying step (S10), the Chamaecyparis obtusa leaves, Platycladus orientalis, Cupressus sempervirens leaves, Cryptomeria japonica leaves, Sabina chinensis xylem, Artemisia capillaris, Dendranthema zawadskii, Angelica acutiloba roots, and Pinus palustris leaves are cleaned and dried.

[0068] In the cleaning and drying step (S10), it is preferred to clean the Chamaecyparis obtusa leaves, Platycladus orientalis, Cupressus sempervirens leaves, Cryptomeria japonica leaves, Sabina chinensis xylem, Artemisia capillaris, Dendranthema zawadskii, Angelica acutiloba roots, and Pinus palustris leaves with purified water, and it is preferred to dry them in a shaded place without direct sunlight for 1 to 3 days.

[0069] Then, the extraction step (S20) is carried out.

[0070] In the above extraction step (S20), the Chamaecyparis obtusa leaves, Platycladus orientalis, Cupressus sempervirens leaves, Cryptomeria japonica leaves, Sabina chinensis xylem, Artemisia capillaris, Dendranthema zawadskii, Angelica acutiloba roots, and Pinus palustris leaves that have been cleaned and dried in the above cleaning and drying step (S10) are extracted using an extraction solvent.

[0071] Preferably, the extraction solvent in the above extraction step (S20) is an extraction solvent selected from the group consisting of water, anhydrous or hydrous lower alcohols having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate, and 1,3 - butanediol. Most preferably, an extraction solvent obtained by mixing ethanol and water is preferably used.

[0072] In the above extraction step (S20), preferably, at a temperature of 60 to 90 °C, using an extraction solvent selected from the group consisting of water, anhydrous or hydrous lower alcohols having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate, and 1,3-butanediol, the cleaned and dried Chamaecyparis obtusa leaves, Platycladus orientalis, Cupressus sempervirens leaves, Cryptomeria japonica leaves, Sabina chinensis xylem, Artemisia capillaris, Dendranthema zawadskii, Angelica acutiloba roots, and Pinus palustris leaves are reflux-extracted 2 to 4 times, preferably 3 times, for 3 to 5 hours each time, preferably 4 hours.

[0073] After that, a powder preparation step (S30) is carried out.

[0074] In the above powder preparation step (S30), the Chamaecyparis obtusa leaf extract, Platycladus orientalis extract, Cupressus sempervirens leaf extract, Cryptomeria japonica leaf extract, Sabina chinensis xylem extract, Artemisia capillaris extract, Dendranthema zawadskii extract, Angelica acutiloba root extract, and Pinus palustris leaf extract extracted in the above extraction step (S20) are made into powder form.

[0075] In the above powder preparation step (S30), preferably, the extracted Chamaecyparis obtusa leaf extract, Platycladus orientalis extract, Cupressus sempervirens leaf extract, Cryptomeria japonica leaf extract, Sabina chinensis xylem extract, Artemisia capillaris extract, Dendranthema zawadskii extract, Angelica acutiloba root extract, and Pinus palustris leaf extract are filtered through Whatman #3 filter paper, and concentrated under reduced pressure at a temperature below 50 °C and freeze-dried to be made into powder form.

[0076] Finally, a mixing step (S40) is carried out.

[0077] In the above mixing step (S40), the Chamaecyparis obtusa leaf extract, Platycladus orientalis extract, Cupressus sempervirens leaf extract, Cryptomeria japonica leaf extract, Sabina chinensis xylem extract, Artemisia capillaris extract, Dendranthema zawadskii extract, Angelica acutiloba root extract, and Pinus palustris leaf extract made into powder form in the above powder preparation step (S30) are mixed.

[0078] In the above mixing step (S40), preferably, the Chamaecyparis obtusa leaf extract, Platycladus orientalis extract, Cupressus sempervirens leaf extract, Cryptomeria japonica leaf extract, Sabina chinensis xylem extract, Artemisia capillaris extract, Dendranthema zawadskii extract, Angelica acutiloba root extract, and Pinus palustris leaf extract made into powder form are mixed at a weight ratio of 0.1 to 10.0:0.1 to 10.0:0.1 to 10.0:0.1 to 10.0:0.1 to 10.0:0.1 to 10.0:0.1 to 10.0:0.1 to 10.0:0.1 to 10.0:0.1 to 10.0.

[0079] Preparation Example 1. Preparation of Chamaecyparis obtusa Leaf Extract

[0080] After washing and drying the Chamaecyparis obtusa leaves, 1 kg of 30% ethanol was added to make the concentration reach 25 g / L, and reflux extraction was carried out 3 times at 60 - 90 °C for 4 hours each time. After cold soaking at room temperature, it was filtered using Whatman #3 filter paper, and concentrated under reduced pressure and freeze-dried at a temperature below 50 °C to obtain the Chamaecyparis obtusa leaf extract.

[0081] Preparation Example 2. Preparation of Platycladus orientalis Extract

[0082] After washing and drying the Platycladus orientalis, 1 kg of 30% ethanol was added to make the concentration reach 25 g / L, and reflux extraction was carried out 3 times at 60 - 90 °C for 4 hours each time. After cold soaking at room temperature, it was filtered using Whatman #3 filter paper, and concentrated under reduced pressure and freeze-dried at a temperature below 50 °C to obtain the Platycladus orientalis extract.

[0083] Preparation Example 3. Cupressus sempervirens L. Leaf Extract

[0084] After washing and drying the Cupressus sempervirens L. leaves, 1 kg of 30% ethanol was added to make the concentration reach 25 g / L, and reflux extraction was carried out 3 times at 60 - 90 °C for 4 hours each time. After cold soaking at room temperature, it was filtered using Whatman #3 filter paper, and concentrated under reduced pressure and freeze-dried at a temperature below 50 °C to obtain the Cupressus sempervirens L. leaf extract.

[0085] Preparation Example 4. Cryptomeria fortunei Hooibrenk ex Otto et Dietr. Leaf Extract

[0086] After washing and drying the Cryptomeria fortunei Hooibrenk ex Otto et Dietr. leaves, 1 kg of 30% ethanol was added to make the concentration reach 25 g / L, and reflux extraction was carried out 3 times at 60 - 90 °C for 4 hours each time. After cold soaking at room temperature, it was filtered using Whatman #3 filter paper, and concentrated under reduced pressure and freeze-dried at a temperature below 50 °C to obtain the Cryptomeria fortunei Hooibrenk ex Otto et Dietr. leaf extract.

[0087] Preparation Example 5. Sabina chinensis (L.) Ant. Xylem Extract

[0088] After washing and drying the Sabina chinensis (L.) Ant. xylem, 1 kg of 30% ethanol was added to make the concentration reach 25 g / L, and reflux extraction was carried out 3 times at 60 - 90 °C for 4 hours each time. After cold soaking at room temperature, it was filtered using Whatman #3 filter paper, and concentrated under reduced pressure and freeze-dried at a temperature below 50 °C to obtain the Sabina chinensis (L.) Ant. xylem extract.

[0089] Preparation Example 6. Artemisia capillaris Thunb. Extract

[0090] After washing and drying Artemisia capillaris, 1 kg of 30% ethanol was added to make the concentration reach 25 g / L, and it was refluxed and extracted 3 times at 60 - 90 °C for 4 hours each time. After cold soaking at room temperature, it was filtered using Whatman #3 filter paper, and concentrated under reduced pressure and freeze-dried at a temperature below 50 °C to obtain the Artemisia capillaris extract.

[0091] Preparation Example 7. Extract of Chrysanthemum zawadskii

[0092] After washing and drying Chrysanthemum zawadskii, 1 kg of 30% ethanol was added to make the concentration reach 25 g / L, and it was refluxed and extracted 3 times at 60 - 90 °C for 4 hours each time. After cold soaking at room temperature, it was filtered using Whatman #3 filter paper, and concentrated under reduced pressure and freeze-dried at a temperature below 50 °C to obtain the Chrysanthemum zawadskii extract.

[0093] Preparation Example 8. Extract of Angelica acutiloba root

[0094] After washing and drying Angelica acutiloba root, 1 kg of 30% ethanol was added to make the concentration reach 25 g / L, and it was refluxed and extracted 3 times at 60 - 90 °C for 4 hours each time. After cold soaking at room temperature, it was filtered using Whatman #3 filter paper, and concentrated under reduced pressure and freeze-dried at a temperature below 50 °C to obtain the Angelica acutiloba root extract.

[0095] Preparation Example 9. Extract of Pinus palustris leaf

[0096] After washing and drying Pinus palustris leaf, 1 kg of 30% ethanol was added to make the concentration reach 25 g / L, and it was refluxed and extracted 3 times at 60 - 90 °C for 4 hours each time. After cold soaking at room temperature, it was filtered using Whatman #3 filter paper, and concentrated under reduced pressure and freeze-dried at a temperature below 50 °C to obtain the Pinus palustris leaf extract.

[0097] Preparation Example 10. Preparation of extraction mixture

[0098] The samples obtained from Preparation Examples 1 to 9 above were mixed in a ratio of 1:1:1:1:1:1:1:1:1 to prepare the extraction mixture (phytoncide extract) of Preparation Example 10.

[0099] Example 1 and Comparative Examples 1 to 9. Preparation of cosmetic compositions

[0100] The cosmetic compositions of Example 1 and Comparative Examples 1 to 9 were prepared according to Table 1 below.

[0101] Table 1

[0102]

[0103]

[0104] Unit: (weight percentage)

[0105] Experimental Example 1. Cell viability test

[0106] The degree of influence on the cell viability of the samples obtained from Preparation Examples 1 to 10 above was measured. After suspending the samples obtained from Preparation Examples 1 to 10 above in purified water to form different concentrations (0.05%, 0.1%, 0.5%, 1.0%, 3.0%, 5.0%), the cell viability was measured by the following method.

[0107] 1) Experimental method

[0108] The cytotoxicity and proliferation experiments were carried out by the following method. The cells in culture (fibroblasts, 3T3 cells) were subcultured at 5000 cells / well in a 96-well microplate and cultured in an incubator for 30 minutes. After administering the samples at different concentrations (0.05%, 0.1%, 0.5%, 1.0%, 3.0%, 5.0%), they were cultured for 72 hours. Then, after administering thiazolyl blue, they were further cultured for 4 hours. After discarding all the culture media, a reaction termination solution was added to each well of the microplate, and after stirring for 5 minutes, the absorbance was measured at 570 nm. A 10% fetal bovine serum (FBS) medium equal to the injection volume of the sample was administered to the control group, and co-culture was carried out under the optimal conditions for cell growth. The cell proliferation rate of the control group was set to 100%, and the cell proliferation rate of the sample injection experimental group was calculated.

[0109] 2) Experimental results

[0110] The cell proliferation effect was calculated by Mathematical Formula 1, and the results are shown in Table 2 below.

[0111]

[0112] Mathematical Formula 1:

[0113] Table 2

[0114]

[0115]

[0116] The experimental results are as shown in Table 2 and Figure 2 It can be seen that compared with Preparation Examples 1 to 9, the cell viability of different treatment concentrations of Preparation Example 10 containing the phytobacteriocin extract is the most excellent.

[0117] Experimental Example 2. Confirmation of free radical scavenging effect

[0118] The free radical scavenging effects of the compositions prepared in Preparation Examples 1 to 10 above were determined.

[0119] 1) Experimental method

[0120] The experiment was carried out using the DPPH (1,1-diphenyl-2-picrylhydrazyl) method (reference: Blois.M.S. Nature 181, 1190, 1958). DPPH and quercetin were from Sigma. In 1 ml of 0.2 mM DPPH methanol solution, the absorbances of Preparation Examples 1 to 10 at different concentrations (0.05%, 0.1%, 0.5%, 1.0%, 3.0%) were measured at 517 nm. At this time, 0.1% ascorbic acid was used instead of each sample as a blank test.

[0121] The free radical scavenging effect was calculated using the following Mathematical Formula 2, and the results are shown in Table 3 below.

[0122]

[0123] Mathematical Formula 2:

[0124] Table 3

[0125]

[0126]

[0127] The experimental results are shown in Table 3 and Figure 3 as can be seen, compared with Preparation Examples 1 to 9, the free radical scavenging effects of different treatment concentrations of Preparation Example 10 containing the phytobacteriocin extract were the most excellent.

[0128] Experimental Example 3. Determination of elastase inhibitory activity

[0129] The elastase inhibitory effects of the compositions obtained from Preparation Examples 1 to 10 above were confirmed.

[0130] Elastase, which is an elastoprotein hydrolase, was purchased from Waco (Japan) Co., Ltd. 100 μl of 5 mM Suc-(Ala)3-PNA and the composition test sample (MeOH solution, 50 μl) obtained from Preparation Examples 1 to 10 above were added to a HEPES buffer solution and left at a temperature of 25 °C for 220 seconds. Then, 20 μl of elastase (to make the intracellular enzyme concentration reach 0.2 unit) was added and left for 60 seconds, and the absorbance was measured at 410 nm. Oleanolic Acid was used as a control group. The inhibition rate was calculated using the following Mathematical Formula 3, and the results are shown in Table 4 below.

[0131]

[0132] Mathematical Formula 3:

[0133] Table 4

[0134]

[0135]

[0136] The experimental results are shown in Table 4 and Figure 4 as follows. It can be seen that compared with Preparation Examples 1 to 9, the elastase inhibition ability of different treatment concentrations of Preparation Example 10 containing the phytobacteriocin extract is the most excellent.

[0137] Experimental Example 4. Determination of Collagenase Inhibitory Activity

[0138] The following experiment was conducted on the inhibitory effect of collagenase, which is closely related to the formation of skin wrinkles, by applying the method described in the following literature. (WE, Heindrich HG. (1963) Zur quantitativen bestimmung der kollagenase. Hoppe-seyler's Zeitschrift Fur Physiologische Chemie. 333: 149 - 151).

[0139] Dissolve 0.25 mL of a substrate solution of 4-phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-DArg (4-phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-DArg, BC13H3755V, Sigma-Aldrich Co., St Louis, MO, USA, 0.3 mg / mL) in 0.1 M Tris-HCl buffer (pH 7.5) containing 4 mM CaCl2, mix it with 0.1 mL of a sample solution diluted to a certain concentration (dilutions of 0.05, 0.1, 0.5, 1.0, 3.0 of the compositions obtained from Preparation Examples 1 to 10), then add 0.15 mL of collagenase (0.2 mg / mL, C0130, Sigma-Aldrich Co., St Louis, MO, USA), and react at room temperature for 20 minutes. After adding 0.5 mL of 6% citric acid (citric acid, 251275, Sigma Aldrich Co., St Louis, MO, USA) to terminate the reaction, add 1.5 mL of ethyl acetate and mix. Only take the supernatant and measure the absorbance at 320 nm. Use EGCG (Epigallocatechin gallate) as the control group. The collagenase inhibition rate is expressed as the percentage (%) of the absorbance reduction rate of the compositions obtained from Preparation Examples 1 to 10 of the sample solution.

[0140] Table 5

[0141]

[0142]

[0143] The experimental results are shown in Table 5 and Figure 5 As shown, compared with Preparation Examples 1 to 9, the collagenase inhibitory ability of different treatment concentrations of Preparation Example 10 containing the phytoalexin extract is the most excellent.

[0144] Experimental Example 5. Skin moisturizing improvement effect

[0145] 1) Experimental method

[0146] The cosmetic compositions prepared in the above Example 1 and Comparative Examples 1 to 9 were respectively applied to the two sides and the front half of the faces of 30 subjects (male and female aged between 20 and 45 years old), twice a day for 4 weeks. Before applying the cosmetic compositions prepared in Example 1 and Comparative Examples 1 to 9, 2 weeks after application, and 4 weeks after application, a skin moisture tester (Corneometer CM 820, Courage+Khazaka, Germany) was used to measure the improved state of skin moisturization, and the unit was expressed in arbitrary units (AU) given by the device. The higher the measured value, the higher the water content on the skin surface.

[0147] 2) Experimental results

[0148] The experimental results regarding the improvement of skin moisturization are shown in Table 6 below.

[0149] Table 6

[0150]

[0151]

[0152] The experimental results are shown in Table 6 and Figure 6 as shown. It can be seen that, compared with Comparative Examples 1 to 9, the skin moisturization improvement effect of Example 1 containing the phytobacteriocin extract is the most excellent.

[0153] Experimental Example 6. Evaluation of transepidermal water loss

[0154] 1) Experimental method

[0155] The cosmetic compositions prepared in the above Example 1 and Comparative Examples 1 to 9 were respectively applied to 30 subjects (male and female aged between 20 and 45 years old). The upper arm part of the subjects was set as the measurement part. After marking the test part with a size of 2×2 cm in length and width 2 , measurement was carried out. After applying the cosmetic composition to the test part, it was rubbed thoroughly, and a transepidermal water loss measuring instrument (TEWAMETER TM210, C+K eletronic GmhH.Germany) was used to measure the transepidermal water loss. Based on the water retention amount at 0 hour before application, the water content (%) was measured 30 minutes after application, 1 day after application, 4 days after application, and 8 days after application, so as to evaluate the short-term moisturization effect. During measurement, the probe was vertically in contact and kept horizontal for 60 seconds, and the transepidermal water loss was measured continuously 3 times. Measurement was carried out in a constant temperature and humidity chamber in the same way. The smaller the value, the less the water loss.

[0156] Table 7

[0157]

[0158]

[0159] The experimental results are shown in Table 7 and Figure 7 as follows. Compared with Comparative Examples 1 to 9, Example 1 containing the phytobacteriocin extract lost the least amount of water over time. Thus, it can be seen that Example 1 containing the phytobacteriocin extract had the least amount of transdermal water loss.

[0160] Formulation Example 1. Preparation of a toner formulation

[0161] The formulation of the toner using the phytobacteriocin extracts of Preparation Examples 1 to 9 is shown in Table 8.

[0162] Table 8

[0163]

[0164]

[0165] Formulation Example 2. Preparation of a lotion formulation

[0166] The formulation of the lotion using the phytobacteriocin extracts of Preparation Examples 1 to 9 is shown in Table 9.

[0167] Table 9

[0168]

[0169]

[0170] Formulation Example 3. Preparation of a cream formulation

[0171] The formulation of the cream using the phytobacteriocin extracts of Preparation Examples 1 to 9 is shown in Table 10.

[0172] Table 10

[0173]

[0174]

[0175] Although the present invention has been described in conjunction with the accompanying drawings, this is merely one embodiment among various embodiments that include the gist of the present invention, and is intended to enable those skilled in the art to easily implement it. Obviously, the present invention is not limited to the above embodiments. Therefore, the protection scope of the present invention shall be subject to the scope of the appended claims for the invention, and all technical concepts within the scope equivalent to the changes, substitutions, replacements, etc. made without departing from the gist of the present invention shall be included in the protection scope of the present invention. And obviously, some structures in the drawings are exaggerated or reduced compared to the actual structures to more clearly illustrate the structures.

Claims

1. A plant bactericidal cosmetic composition, characterized in that, A phytocide extract containing hinoki leaf extract, oriental arborvitae leaf extract, Mediterranean cypress leaf extract, Japanese cedar leaf extract, Chinese juniper xylem extract, Artemisia capillaris extract, wild chrysanthemum extract, extremely fine angelica root extract and longleaf pine leaf extract in a weight ratio of 0.1 to 10.0:0.1 to 10.0:0.1 to 10.0:0.1 to 10.0:0.1 to 10.0:0.1 to 10.0:0.1 to 10.0:0.1 to 10.0:0.1 to 10.0, wherein the above hinoki leaf extract, oriental arborvitae leaf extract, Mediterranean cypress leaf extract, Japanese cedar leaf extract, Chinese juniper xylem extract, Artemisia capillaris extract, wild chrysanthemum extract, extremely fine angelica root extract and longleaf pine leaf extract are extracted using an extraction solvent selected from the group consisting of water and anhydrous or hydrous lower alcohols having 1 to 4 carbon atoms.

2. The phytobactericide cosmetic composition according to claim 1, characterized in that, The above phytocide extract is contained in an amount of 0.01 weight percent to 20.0 weight percent relative to the total weight of the cosmetic composition.

3. Use of the phytoalexin extract in the preparation of a phytoalexin cosmetic composition having skin moisturizing effect, skin antioxidant effect and skin elasticity improving effect, wherein, The phytocide extract is obtained by mixing hinoki leaf extract, oriental arborvitae leaf extract, Mediterranean cypress leaf extract, Japanese cedar leaf extract, Chinese juniper xylem extract, Artemisia capillaris extract, wild chrysanthemum extract, extremely fine angelica root extract and longleaf pine leaf extract in a weight ratio of 0.1 to 10.0:0.1 to 10.0:0.1 to 10.0:0.1 to 10.0:0.1 to 10.0:0.1 to 10.0:0.1 to 10.0:0.1 to 10.0:0.1 to 10.0, and wherein the above hinoki leaf extract, oriental arborvitae leaf extract, Mediterranean cypress leaf extract, Japanese cedar leaf extract, Chinese juniper xylem extract, Artemisia capillaris extract, wild chrysanthemum extract, extremely fine angelica root extract and longleaf pine leaf extract are extracted using an extraction solvent selected from the group consisting of water and anhydrous or hydrous lower alcohols having 1 to 4 carbon atoms.

4. A method for preparing a plant bacteriocin cosmetic composition, characterized in that, Comprising: A cleaning and drying step (S10) of cleaning and drying hinoki leaf, oriental arborvitae, Mediterranean cypress leaf, Japanese cedar leaf, Chinese juniper xylem, Artemisia capillaris, wild chrysanthemum, extremely fine angelica root and longleaf pine leaf; An extraction step (S20) of extracting the hinoki leaf, oriental arborvitae, Mediterranean cypress leaf, Japanese cedar leaf, Chinese juniper xylem, Artemisia capillaris, wild chrysanthemum, extremely fine angelica root and longleaf pine leaf that have been cleaned and dried in the above cleaning and drying step (S10) using an extraction solvent, wherein the extraction solvent is an extraction solvent selected from the group consisting of water and anhydrous or hydrous lower alcohols having 1 to 4 carbon atoms; A powder preparation step (S30) of making the hinoki leaf extract, oriental arborvitae leaf extract, Mediterranean cypress leaf extract, Japanese cedar leaf extract, Chinese juniper xylem extract, Artemisia capillaris extract, wild chrysanthemum extract, extremely fine angelica root extract and longleaf pine leaf extract extracted in the above extraction step (S20) into a powder form; And Mixing step (S40): Mix the hinoki leaf extract, oriental arborvitae leaf extract, Mediterranean cypress leaf extract, Japanese cedar leaf extract, Chinese juniper xylem extract, Artemisia capillaris extract, purple-flowered wild chrysanthemum extract, extremely fine angelica root extract, and longleaf pine leaf extract prepared in powder form in the above powder preparation step (S30) at a weight ratio of 0.1 to 10.0:0.1 to 10.0:0.1 to 10.0:0.1 to 10.0:0.1 to 10.0:0.1 to 10.0:0.1 to 10.0:0.1 to 10.0:0.1 to 10.

0.

5. The preparation method of the phytobactericide cosmetic composition according to claim 4, characterized in that, At a temperature of 60 to 90 °C, reflux extract the hinoki leaf, oriental arborvitae leaf, Mediterranean cypress leaf, Japanese cedar leaf, Chinese juniper xylem, Artemisia capillaris, purple-flowered wild chrysanthemum, extremely fine angelica root, and longleaf pine leaf that have been washed and dried in the above extraction step (S20) 2 to 4 times, each time for 3 to 5 hours, and cool to room temperature.

6. The preparation method of the phytobactericide cosmetic composition according to claim 4, characterized in that, Filter the hinoki leaf extract, oriental arborvitae leaf extract, Mediterranean cypress leaf extract, Japanese cedar leaf extract, Chinese juniper xylem extract, Artemisia capillaris extract, purple-flowered wild chrysanthemum extract, extremely fine angelica root extract, and longleaf pine leaf extract extracted in the above powder preparation step (S30) with filter paper, and concentrate under reduced pressure at a temperature below 50 °C and freeze-dry to form a powder.

Images