Composition, preparation method and application having anti-breakage, repair and nourishing effects

Abstract

The present invention relates to the field of daily-use cosmetics, and particularly relates to a composition with anti-hair-breakage, repair and nourishing effects, a preparation method and an application thereof. The preparation method of the composition with anti-hair-breakage, repair and nourishing effects provided by the present invention comprises the following steps: S1 Preparation of a plant fermentation matrix: uniformly mixing fermentation raw materials, a carbon source, a nitrogen source, inorganic salts and water, sterilizing at high pressure and high temperature and then cooling down to obtain a plant fermentation matrix; S2 Preparation of a mixed fermentation broth: inoculating a Lactococcus lactis subsp. lactis seed solution and a Lactobacillus johnsonii seed solution into the plant fermentation matrix obtained in step S1 and performing fermentation to obtain a mixed fermentation broth; S3 Refining process: performing inactivation treatment and filtration treatment on the mixed fermentation broth obtained in step S2 to obtain a composition with anti-hair-breakage, repair and nourishing effects; the fermentation raw materials in step S1 include white birch bark powder and Myrothamnus flabellifolius powder. The composition provided by the present invention exhibits excellent anti-hair-breakage, repair and nourishing effects.

Description

Technical Field

[0001] The present invention relates to the field of daily-use cosmetics, and particularly relates to a composition, a preparation method and an application having the effects of preventing hair breakage, repairing and nourishing. Background Art

[0002] Hair refers to the hair on the top of the head and the back of the head. Hair can be divided into four parts from bottom to top: hair papilla, hair follicle, hair root and hair shaft. The physiological characteristics and functions of hair mainly depend on the hair papilla, hair follicle and sebaceous gland under the scalp epidermis. For normal and healthy hair, there is a hair core in the middle and it is protected by hair cutin on the outside. When the hair cutin is firm enough, it can open to let nutrients in and supplement nutrition for the hair well, and at the same time, it can close tightly to resist external damage. With the development of the beauty industry, hair beauty behaviors such as perming and dyeing will greatly damage the hair and scalp to a large extent, causing the hair cutin to be damaged and resulting in problems such as dry, brittle and frizzy hair quality. Various hair care products such as hair shampoos and hair essence liquids have emerged in the market.

[0003] At present, the active ingredients in hair care products on the market are mainly chemical synthetic substances. For example, CN102525846A discloses a hair care composition and its preparation method. The hair care composition includes lactate, pyrrolidone carboxylate, amino-modified silicone, volatile silicone, and an aqueous carrier; the aqueous carrier includes components such as cationic surfactant, aliphatic compound and deionized water. CN113876608A discloses a shampoo with mild, smooth, special repair and anti-dandruff effects, including components with the following weight percentages: 10-20% of α-olefin sulfonate, 1-5% of N-acyl glutamate, 0.3-5% of anti-jelly thickener, 1-5% of sodium benzoate, 1-2% of trihydroxyethyl methyl ammonium methyl sulfate, 0.5-1% of piroctone ethanolamine salt, 0.5-1% of sodium benzoate, 0.5-1% of acid-base buffer, 0.05-0.1% of pigment, 0.5-1% of essence, and the balance is made up to 100% with water. The existing hair care products have unsatisfactory care effects on hair, and may also cause various subtle harms to the body after long-term use, while there are few reports on hair care active substances containing natural ingredients and being safe and non-irritating. Summary of the Invention

[0004] Aiming at the deficiencies of the prior art, the purpose of the present invention is to provide a composition, a preparation method and an application having the effects of preventing hair breakage, repairing and nourishing. The composition having the effects of preventing hair breakage, repairing and nourishing provided by the present invention is a hair care active substance with natural ingredients, safe and non-irritating, showing excellent effects of preventing hair breakage, repairing and nourishing. When it is applied to hair products, the use effect is excellent.

[0005] The technical solution of the present invention is as follows:

[0006] A method for preparing a composition with anti - hair - breakage, repair and nourishing effects, comprising the following steps:

[0007] S1 Preparation of plant fermentation matrix: Mix the fermentation raw materials, carbon source, nitrogen source, inorganic salts and water evenly, sterilize at high pressure and high temperature, and then cool down to obtain the plant fermentation matrix;

[0008] S2 Preparation of mixed fermentation broth: Inoculate the Lactococcus lactis subsp. lactis seed liquid and the Lactobacillus johnsonii seed liquid into the plant fermentation matrix described in step S1, and carry out fermentation to obtain the mixed fermentation broth;

[0009] S3 Refining process: Inactivate and filter the mixed fermentation broth described in step S2 to obtain a composition with anti - hair - breakage, repair and nourishing effects;

[0010] The fermentation raw materials described in step S1 include white birch bark powder and Myrothamnus flabellifolius powder.

[0011] Preferably, the Myrothamnus flabellifolius powder is Myrothamnus flabellifolius leaf / stem powder.

[0012] Further, the preparation method of the white birch bark powder is: Grind the dried white birch bark with a grinding machine, sieve through a 50 - 120 mesh sieve, remove the upper impurities, and take the lower powder as the fermentation raw material.

[0013] Further, the preparation method of the Myrothamnus flabellifolius powder is: Grind the dried Myrothamnus flabellifolius leaf / stem (i.e., the Myrothamnus flabellifolius plant without roots) with a grinding machine, sieve through a 50 - 120 mesh sieve, remove the upper impurities, and take the lower powder as the fermentation raw material.

[0014] Further, the total addition amount of the white birch bark powder and the Myrothamnus flabellifolius powder is 0.5 - 2.0% of the mass of the plant fermentation matrix, preferably 1.2%; wherein, the mass ratio of the white birch bark powder to the Myrothamnus flabellifolius powder is 3 - 7:1; preferably 5:1.

[0015] Further, the carbon source is selected from at least one of glucose, sucrose, fructose syrup, maltose, preferably maltose; the addition amount of the carbon source is 0.5 - 2% of the mass of the plant fermentation matrix, preferably 1%.

[0016] Further, the nitrogen source is selected from at least one of corn steep liquor, soybean cake powder, peanut cake powder, yeast extract, preferably soybean cake powder; the addition amount of the nitrogen source is 0.5 - 2% of the mass of the plant fermentation matrix, preferably 1.2%.

[0017] Further, the inorganic salt is selected from at least one of NaCl, Na2HPO4, NaH2PO4, KCl, K2HPO4, and KH2PO4, preferably KH2PO4; the addition amount of the inorganic salt is 0.05-0.5% of the mass of the plant fermentation substrate, preferably 0.15%.

[0018] Further, the total inoculation amount of the Lactococcus lactis subsp. lactis seed liquid and the Lactobacillus johnsonii seed liquid in step S2 is 1%-3% of the mass of the plant fermentation substrate, preferably 1.5%; wherein the mass ratio of the inoculation amounts of the Lactococcus lactis subsp. lactis seed liquid and the Lactobacillus johnsonii seed liquid is 1-4:1, preferably 2:1.

[0019] Further, the preparation method of the Lactococcus lactis subsp. lactis seed liquid is as follows:

[0020] Use an inoculation loop to pick colonies of Lactococcus lactis subsp. lactis into a liquid medium, and culture at 30-42°C and 100-300 rpm for 24-72 h to obtain a Lactococcus lactis subsp. lactis seed liquid with a bacterial concentration of 1×10 7 ~1×10 9 cfu / ml; preferably, culture at 37°C and 200 rpm for 48 h to obtain a Lactococcus lactis subsp. lactis seed liquid with a bacterial concentration of 1×10 8 cfu / ml.

[0021] Further, the preparation method of the Lactobacillus johnsonii seed liquid is as follows:

[0022] Use an inoculation loop to pick colonies of Lactobacillus johnsonii into a liquid medium, and culture at 30-42°C and 100-300 rpm for 24-72 h to obtain a Lactobacillus johnsonii seed liquid with a bacterial concentration of 1×10 7 ~1×10 9 cfu / ml; preferably, culture at 37°C and 200 rpm for 48 h to obtain a Lactobacillus johnsonii seed liquid with a bacterial concentration of 1×10 8 cfu / ml.

[0023] Further, the liquid media for culturing the Lactococcus lactis subsp. lactis seed liquid and the Lactobacillus johnsonii seed liquid are both MRS broth media, and the preparation method of the MRS broth medium is as follows:

[0024] Mix 10 g of peptone, 5 g of beef powder, 4 g of yeast powder, 20 g of glucose, 1 mL of Tween 80, 2 g of dipotassium hydrogen phosphate, 5 g of sodium acetate, 2 g of ammonium citrate, 0.2 g of magnesium sulfate, and 0.05 g of manganese sulfate, add distilled water to 1 L, sterilize at 120°C for 20 min, and store for later use.

[0025] Further, the specific conditions for the fermentation in step S2 are as follows: the fermentation temperature is 35 - 42°C, natural pH (which means that during the fermentation process, the pH of the fermentation broth does not need to be adjusted or monitored, and the fermentation can be completed in 36 - 72 hours), the rotation speed is 100 - 250 rpm, and the fermentation time is 36 - 72 hours.

[0026] Preferably, the specific conditions for the fermentation in step S2 are: the fermentation temperature is 37°C, natural pH, the rotation speed is 150 rpm, and the fermentation time is 48 hours.

[0027] Further, the temperature for the inactivation treatment in step S3 is 85 - 115°C, and the time for the inactivation treatment is 15 - 30 minutes; preferably, the temperature for the inactivation treatment is 90°C, and the time for the inactivation treatment is 20 minutes.

[0028] Further, the filtration treatment in step S3 is carried out using a diatomaceous earth filter or a microfiltration membrane, and preferably, the mixed fermentation broth is filtered through a diatomaceous earth filter.

[0029] Another object of the present invention is to provide a composition having the effects of preventing hair breakage, repairing, and nourishing, which is prepared by the preparation method of the above composition having the effects of preventing hair breakage, repairing, and nourishing.

[0030] Another object of the present invention is to provide the application of the composition having the effects of preventing hair breakage, repairing, and nourishing in the preparation of hair care products.

[0031] The composition of the present invention can be prepared into any preparation conventionally prepared in the field of hair care products, and can be formulated into, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, sprays, oils, etc., but the present invention is not limited thereto. More specifically, the composition of the present invention can be prepared into the following preparations: hair tonic, nourishing cream, hair massage cream, essence, hair cleansing cream, hair cleansing foam, hair cleansing water, hair moisturizing cream, shampoo, hair conditioner, spray or powder hair care products.

[0032] Betula platyphylla belongs to the Betulaceae family and the Betula genus. Betula platyphylla has unique medicinal value and is very beneficial to human health. It has pharmacological effects such as anti - fatigue and cough - relieving, and is called "natural beer" and "forest beverage". The bark of Betula platyphylla has the effects of clearing heat and detoxifying, relaxing muscles and activating collaterals, relieving cough, and promoting diuresis. It can be used internally to treat acute tonsillitis, bronchitis, pneumonia, enteritis dysentery, hepatitis, and acute mastitis, and externally to treat burns, scalds, carbuncles, and furuncles. Existing research shows that Betula platyphylla contains chemical substances such as triterpenoids, arylbutanes and diarylheptanes, flavonoids, lignans, and steroids, which have activities such as anti - inflammation, anti - virus, inhibiting the dissolution of proteins in hair fibers, improving the luster of damaged hair, and promoting hair growth.

[0033] Myrothamnus flabellifolius is also known as Phoenix Herb of South Africa and Resurrection Herb. Myrothamnus flabellifolius is the only woody plant in the world that can be revived and has extremely strong drought tolerance. During winter, the seemingly dried-up and dead leaves can quickly revive after a little rain. Myrothamnus flabellifolius extract can quickly repair damaged cells, enhance cell vitality, and protect the skin when exposed to strong sunlight.

[0034] The present invention selects Betula platyphylla bark and Myrothamnus flabellifolius leaves / stems (selecting Myrothamnus flabellifolius plants without roots, i.e., the Myrothamnus flabellifolius leaves / stems part) to prepare a plant fermentation matrix, and uses two different lactic acid bacteria to carry out symbiotic fermentation on Betula platyphylla bark and Myrothamnus flabellifolius. The obtained composition is rich in active substances with the effects of preventing hair breakage, repairing, and nourishing, and is suitable as an active substance for hair products. In addition, the present invention uses probiotics to ferment and extract plants, and the obtained composition is safe, pollution-free to the environment, and non-irritating to the human body.

[0035] Compared with the prior art, the composition with the effects of preventing hair breakage, repairing, and nourishing prepared by the preparation method provided by the present invention has the following advantages:

[0036] (1) The composition prepared by the symbiotic fermentation method of the present invention is rich in active substances in Betula platyphylla bark and Myrothamnus flabellifolius. Compared with the case of using only one of the lactic acid bacteria for fermentation alone, when two different lactic acid bacteria are used for symbiotic fermentation of Betula platyphylla bark and Myrothamnus flabellifolius, there is a synergistic effect between the two different lactic acid bacteria, making the obtained composition show more excellent effects of preventing hair breakage, repairing, and nourishing.

[0037] (2) Fermenting Betula platyphylla bark and Myrothamnus flabellifolius together not only avoids fermenting each plant alone, greatly simplifies the preparation process, shortens the process time, and reduces the production cost, but also there is a synergistic effect between the two plants, and the effect of the obtained composition is better than that of single-plant fermentation.

[0038] (3) The composition of the present invention does not use any organic solvents during the preparation process, is harmless to human safety and the environment, and is conducive to environmental protection. Specific Embodiments

[0039] The present invention will be further described below through the description of specific embodiments, but this is not a limitation of the present invention. Those skilled in the art can make various modifications or improvements according to the basic idea of the present invention, but as long as they do not depart from the basic idea of the present invention, they are within the scope of the present invention.

[0040] The raw materials used in the present invention are commercially available unless otherwise specified. In the examples of the present invention, Lactococcus lactis subsp. lactis used is Lactococcus lactis subsp. lactis (Bio-67315), purchased from Beijing Bio-win Biotechnology Co., Ltd.; Lactobacillus johnsonii is Lactobacillus johnsonii (GDMCC 1.730), purchased from Guangdong Microbial Culture Collection Center; Saccharomyces cerevisiae is Saccharomyces cerevisiae (BNCC336054), purchased from Beijing Beina Chuanglian Biotechnology Research Institute.

[0041] It should be noted that Lactococcus lactis subsp. lactis and Lactobacillus johnsonii obtained through commercial channels can both be used to prepare the composition with the effects of preventing hair breakage, repairing and nourishing of the present invention, and are not limited to Lactococcus lactis subsp. lactis (Bio-67315) and Lactobacillus johnsonii (GDMCC 1.730) mentioned above.

[0042] Example 1

[0043] A composition with the effects of preventing hair breakage, repairing and nourishing is prepared by the following method:

[0044] 1) Prepare the fermentation raw materials

[0045] Dry white birch bark is ground into powder with a grinding machine, passed through an 80-mesh sieve, the upper impurities are removed, and the lower powder is taken as the fermentation raw material (white birch bark powder);

[0046] Dry Myrothamnus flabellifolius leaves / stems (i.e., Myrothamnus flabellifolius plants without roots) are ground into powder with a grinding machine, passed through an 80-mesh sieve, the upper impurities are removed, and the lower powder is taken as the fermentation raw material (Myrothamnus flabellifolius powder).

[0047] 2) Prepare the Lactococcus lactis subsp. lactis seed liquid and the Lactobacillus johnsonii seed liquid

[0048] A loopful of Lactococcus lactis subsp. lactis colonies is picked and transferred into MRS broth medium, and cultured at 37 °C and 200 rpm for 48 h to obtain a Lactococcus lactis subsp. lactis seed liquid with a cell concentration of 1×10 8 cfu / ml.

[0049] A loopful of Lactobacillus johnsonii colonies is picked and transferred into MRS broth medium, and cultured at 37 °C and 200 rpm for 48 h to obtain a Lactobacillus johnsonii seed liquid with a cell concentration of 1×10 8 cfu / ml.

[0050] 3) Prepare the plant fermentation matrix

[0051] The plant fermentation matrix comprises the following components in mass percentage: 1% of white birch bark powder, 0.2% of Myrothamnus flabellifolius powder, 1% of maltose, 1.2% of soybean cake powder, 0.15% of KH2PO4, and 96.45% of water.

[0052] Dissolve birch bark powder, myrothamnus flabellifolius powder, maltose, soybean cake powder and KH2PO4 in water, mix well, seal the fermenter, heat up to 121 °C, maintain for 30 min for sterilization treatment, and cool down to room temperature to obtain a plant fermentation matrix.

[0053] 4) Prepare a mixed fermentation broth

[0054] Inoculate the Lactococcus lactis subsp. lactis seed liquid and the Lactobacillus johnsonii seed liquid into the plant fermentation matrix. The inoculation amount of the Lactococcus lactis subsp. lactis seed liquid is 1% of the mass of the plant fermentation matrix, and the inoculation amount of the Lactobacillus johnsonii seed liquid is 0.5% of the mass of the plant fermentation matrix. Ferment at 37 °C, natural pH, and a stirring paddle speed of 150 rpm for 48 h to obtain a mixed fermentation broth.

[0055] 5) Refining process

[0056] Inactivate the mixed fermentation broth obtained in step 4) at 90 °C for 20 min, and then filter the inactivated mixed fermentation broth through a diatomaceous earth filter to obtain a composition with the effects of preventing hair breakage, repairing and nourishing.

[0057] Examples 2 - 7, Comparative Examples 1 - 8

[0058] The preparation methods of the compositions with the effects of preventing hair breakage, repairing and nourishing in Examples 2 - 7 and Comparative Examples 1 - 8 are the same as those in Example 1, except that the fermentation raw materials and their addition amounts, carbon sources, nitrogen sources and their addition amounts, and the types of strains and their inoculation amounts in steps 1) - 4) are different; the differences in Examples 2 - 7 and Comparative Examples 1 - 8 are shown in Table 1.

[0059] Table 1: Differences between Examples 2 - 7 and Comparative Examples 1 - 8 and Example 1

[0060]

[0061]

[0062] Example 8

[0063] A composition with the effects of preventing hair breakage, repairing and nourishing is prepared by the following method:

[0064] 1) Prepare fermentation raw materials

[0065] Powder the dried birch bark with a grinding machine, pass through a 50 - mesh sieve, remove the upper - layer impurities, and take the lower - layer powder as the fermentation raw material (birch bark powder);

[0066] Powder the dry Myrothamnus flabellifolius leaves / stems (i.e., Myrothamnus flabellifolius plants without roots) using a grinding machine, sieve through a 50-mesh sieve, remove the upper impurities, and take the lower powder as the fermentation raw material (Myrothamnus flabellifolius powder).

[0067] 2) Prepare the Lactococcus lactis subsp. lactis seed liquid and the Lactobacillus johnsonii seed liquid

[0068] Use an inoculation loop to pick the Lactococcus lactis subsp. lactis colony into the MRS broth medium and culture it at 30 °C and 300 rpm for 24 h to obtain a Lactococcus lactis subsp. lactis seed liquid with a bacterial concentration of 1×10 7 cfu / ml.

[0069] Use an inoculation loop to pick the Lactobacillus johnsonii colony into the MRS broth medium and culture it at 30 °C and 300 rpm for 24 h to obtain a Lactobacillus johnsonii seed liquid with a bacterial concentration of 1×10 7 cfu / ml.

[0070] 3) Prepare the plant fermentation substrate

[0071] The plant fermentation substrate includes the following components by mass percentage: Betula platyphylla bark powder 1.6%, Myrothamnus flabellifolius powder 0.4%, maltose 2%, soybean cake powder 0.5%, NaCl 0.5%, and water 95%.

[0072] Dissolve the Betula platyphylla bark powder, Myrothamnus flabellifolius powder, maltose, soybean cake powder, and NaCl in water, mix evenly, seal the fermentation tank, heat up to 121 °C, maintain for 30 min for sterilization treatment, and cool down to room temperature to obtain the plant fermentation substrate.

[0073] 4) Prepare the mixed fermentation broth

[0074] Inoculate the Lactococcus lactis subsp. lactis seed liquid and the Lactobacillus johnsonii seed liquid into the plant fermentation substrate. The inoculation amount of the Lactococcus lactis subsp. lactis seed liquid is 2% of the mass of the plant fermentation substrate, and the inoculation amount of the Lactobacillus johnsonii seed liquid is 1% of the mass of the plant fermentation substrate. Ferment at 42 °C, natural pH, and a stirring paddle speed of 120 rpm for 72 h to obtain the mixed fermentation broth.

[0075] 5) Refining process

[0076] Inactivate the mixed fermentation broth obtained in step 4) at 115 °C for 15 min, and then filter the inactivated mixed fermentation broth through a 2.5-μm polypropylene microfiltration membrane filter column to remove impurities to obtain a composition with the effects of preventing hair breakage, repairing, and nourishing.

[0077] Example 9

[0078] A composition with the effects of preventing hair breakage, repairing, and nourishing is prepared by the following method:

[0079] 1) Preparation of fermentation raw materials

[0080] Powder the dried Betula platyphylla bark with a grinding machine, sieve it through a 120-mesh sieve, remove the upper impurities, and take the lower powder as the fermentation raw material (Betula platyphylla bark powder);

[0081] Powder the dried leaves / stems of Myrsine seguinii (i.e., Myrsine seguinii plants without roots) with a grinding machine, sieve it through a 120-mesh sieve, remove the upper impurities, and take the lower powder as the fermentation raw material (Myrsine seguinii powder).

[0082] 2) Preparation of Lactococcus lactis subsp. lactis seed liquid and Lactobacillus johnsonii seed liquid

[0083] Use an inoculation loop to pick Lactococcus lactis subsp. lactis colonies into MRS broth medium, and culture them at 42 °C and 100 rpm for 72 h to obtain a Lactococcus lactis subsp. lactis seed liquid with a bacterial concentration of 1×10 9 cfu / ml.

[0084] Use an inoculation loop to pick Lactobacillus johnsonii colonies into MRS broth medium, and culture them at 42 °C and 100 rpm for 72 h to obtain a Lactobacillus johnsonii seed liquid with a bacterial concentration of 1×10 9 cfu / ml.

[0085] 3) Preparation of plant fermentation substrate

[0086] The plant fermentation substrate includes the following components in mass percentage: Betula platyphylla bark powder 0.375%, Myrsine seguinii powder 0.125%, maltose 0.5%, soybean cake powder 2%, Na2HPO4 0.05%, water 96.95%.

[0087] Dissolve Betula platyphylla bark powder, Myrsine seguinii powder, maltose, soybean cake powder and Na2HPO4 in water, mix evenly, seal the fermentation tank, heat up to 121 °C, keep it for 30 min for sterilization treatment, and cool down to room temperature to obtain the plant fermentation substrate.

[0088] 4) Preparation of mixed fermentation broth

[0089] Inoculate the Lactococcus lactis subsp. lactis seed liquid and Lactobacillus johnsonii seed liquid into the plant fermentation substrate. The inoculation amount of the Lactococcus lactis subsp. lactis seed liquid is 0.8% of the mass of the plant fermentation substrate, and the inoculation amount of the Lactobacillus johnsonii seed liquid is 0.2% of the mass of the plant fermentation substrate. Ferment at 35 °C, natural pH, and a stirring paddle speed of 200 rpm for 72 h to obtain the mixed fermentation broth.

[0090] 5) Refining process

[0091] The mixed fermentation broth obtained in step 4) is inactivated at 85°C for 30 minutes, and then the inactivated mixed fermentation broth is filtered through a diatomaceous earth filter to obtain a composition with the effects of preventing hair breakage, repairing, and nourishing.

[0092] Application Example

[0093] The hair care essence is prepared from the above Examples 1-9 and Comparative Examples 1-8 according to the formulations in Table 2.

[0094] Table 2: Formulation of Hair Care Essence

[0095]

[0096]

[0097] The materials in Phase A are mixed evenly at 70°C and kept warm for later use to obtain mixed solution A; the materials in Phase B are mixed evenly at 70°C and kept warm for later use to obtain mixed solution B; at 80°C, mixed solution B is added to mixed solution A and mixed evenly; the temperature is lowered to 45°C, and the materials in Phase C are added and stirred evenly to prepare the hair care essence.

[0098] Experimental Example 1: Multiple Skin Irritation Test

[0099] The composition with the effects of preventing hair breakage, repairing, and nourishing prepared in the present invention is subjected to a multiple skin irritation test with reference to the method in "Technical Specifications for Cosmetics Safety" (2015 Edition).

[0100] 68 white rabbits meeting the test conditions are prepared (4 white rabbits are used for each sample), and the hair on both sides of the spine on the back of the rabbits is cut off before the test (the depilated area is 3 cm * 3 cm for each).

[0101] Take 0.5 mL of the composition with the effects of preventing hair breakage, repairing, and nourishing from Examples 1-9 and the compositions from Comparative Examples 1-8 and apply them on one side of the skin (the application area is 2.5 cm * 2.5 cm), and the other side is not treated as a control. Apply once a day for 14 consecutive days. Starting from the second day, cut the hair before each application. Remove the residual test substance with pure water. Observe the results 1 hour later.

[0102] Score according to Table 1 of the Skin Irritation / Corrosion Test in "Technical Specifications for Cosmetics Safety" (2015 Edition), and treat the control area and the test area in the same way.

[0103] Result evaluation: Calculate the average score per rabbit per day according to the following formula. The average score per rabbit per day = (erythema and edema score / number of test animals) / 14. Determine the skin irritation intensity of rabbits according to Table 2 of the Skin Irritation / Corrosion Test in the Cosmetics Safety and Technology Specifications (2015 Edition). Observe whether there are other symptoms on the skin other than skin irritation during the test. The results of multiple skin irritation tests of the compositions with anti-hair breakage, repair, and nourishing effects in Examples 1-9 and the compositions in Comparative Examples 1-8 are shown in Table 3.

[0104] Table 3: Results of Multiple Skin Irritation Tests

[0105] Sample Results of multiple skin irritations Example 1 Non-irritating Example 2 Non-irritating Example 3 Non-irritating Example 4 Non-irritating Example 5 Non-irritating Example 6 Non-irritating Example 7 Non-irritating Example 8 Non-irritating Example 9 Non-irritating Comparative Example 1 Non-irritating Comparative Example 2 Non-irritating Comparative Example 3 Non-irritating Comparative Example 4 Non-irritating Comparative Example 5 Non-irritating Comparative Example 6 Non-irritating Comparative Example 7 Non-irritating Comparative Example 8 Non-irritating

[0106] It can be seen from the results of multiple skin irritation tests in Table 3 that the compositions obtained in Examples 1-9 of the present invention and Comparative Examples 1-8 have no irritation and high safety.

[0107] Test Example 2: Test on the Efficacy of Hair Toughness

[0108] Test the ability of hair strands to resist stretching or breaking through the "Evaluation Method for the Efficacy of Strengthening Hair Products T / GDCDC 012-2020" to evaluate the anti-hair breakage and hair-strengthening efficacy of products.

[0109] Test method: Select 180 volunteers with hair quality damage (matrix essence, hair care essence in Examples 1-9 and Comparative Examples 1-8, 10 people in each group), and use the hair care essence in Examples 1-9, the hair care essence in Comparative Examples 1-8, and the matrix essence as test samples respectively. The volunteers select an appropriate amount of use, apply the hair care essence evenly on the hair strands, use it once a day for a total of 30 days. Before and after using the samples, randomly select the hair strands of the volunteers for single-strand tensile testing, record the values of the breaking work before and after using the test products, calculate the change rate of the breaking work, and judge the ability of the test samples to improve the toughness of the hair strands and the anti-hair breakage efficacy.

[0110] Instruments used for testing: Hair Multi-Function Testing System (MTT175)

[0111] Change rate of hair strand breaking work (%) = (breaking work of hair strands after using the product - breaking work of hair strands before using the product) / breaking work of hair strands before using the product * 100%. The greater the breaking work of the hair strands, the more difficult it is to break the hair strands. When the change rate of the breaking work of the hair strands is negative, it indicates that the product does not have the efficacy of strengthening the hair strands and preventing hair breakage; when the change rate of the breaking work of the hair strands is positive, it indicates that the product has the efficacy of strengthening the hair strands and preventing hair breakage, and the larger the value, the better the efficacy of strengthening the hair strands and preventing hair breakage of the product.

[0112] The test results are shown in Table 4.

[0113] Table 4: Test Results of Hair Toughness Effect

[0114] Test sample Change rate of hair breakage work (%) Matrix essence 0.2 Example 1 34.2 Example 2 30.5 Example 3 32.2 Example 4 29.8 Example 5 31.7 Example 6 29.6 Example 7 30.4 Example 8 32.8 Example 9 25.5 Comparative Example 1 10.7 Comparative Example 2 3.8 Comparative Example 3 6.3 Comparative Example 4 12.8 Comparative Example 5 15.3 Comparative Example 6 11.6 Comparative Example 7 8.3 Comparative Example 8 20.7

[0115] As shown in the results of Table 4, the anti - hair - breakage effects of Examples 1 - 9 are better than those of Comparative Examples 1 - 8, indicating that during the fermentation process, the types and ratios of fermentation raw materials, the types of carbon sources and nitrogen sources, the types of fermentation strains and their addition ratios have a great impact on the anti - hair - breakage effect of the finally prepared composition. The anti - hair - breakage effect of Example 1 is better than that of Comparative Examples 1 - 4, indicating that there is a synergistic effect between white birch bark and Morinda citrifolia leaf / stem during the fermentation process, and the composition obtained by co - fermenting the two has the best anti - hair - breakage effect. Replacing white birch bark with white birch leaves or replacing Morinda citrifolia leaf / stem with Morinda citrifolia root results in a poorer anti - hair - breakage effect of the prepared composition. The anti - hair - breakage effect of Example 1 is better than that of Comparative Examples 5 - 7, indicating that the type of bacteria has a great impact on the fermentation process. In the present invention, the symbiotic fermentation of Lactococcus lactis subsp. lactis and Lactobacillus johnsonii gives the composition with the best anti - hair - breakage effect. The anti - hair - breakage effect of Example 1 is better than that of Examples 6, 7 and Comparative Example 8, indicating that the inoculation ratio of Lactococcus lactis subsp. lactis and Lactobacillus johnsonii also has a great impact on the fermentation process. In the present invention, the inoculation ratio of the seed solution of Lactococcus lactis subsp. lactis and the seed solution of Lactobacillus johnsonii should be 1 - 4:1, more preferably 2:1. The anti - hair - breakage effect of Example 1 is better than that of Examples 2 and 3. In the present invention, the mass ratio of white birch bark powder to Morinda citrifolia powder should be 3 - 7:1, more preferably 5:1. The anti - hair - breakage effect of Example 1 is better than that of Examples 4 and 5, indicating that the selection of carbon sources and nitrogen sources in the fermentation matrix affects the fermentation effect and thus affects the anti - hair - breakage effect of the composition; in the present invention, the carbon source should be selected from at least one of glucose, sucrose, fructose syrup, and maltose, more preferably maltose; the nitrogen source should be selected from at least one of corn steep liquor, soybean cake powder, peanut cake powder, and yeast extract, more preferably soybean cake powder.

[0116] Test Example 3: Test on Hair Repair and Nourishing Effects

[0117] By testing the combing work, friction coefficient and hair strand glossiness of slightly damaged hair strands, the effects of the product on repairing and nourishing hair are evaluated.

[0118] Test method: 180 volunteers with damaged hair quality were selected (for the hair essence of the matrix essence, Examples 1-9 and Comparative Examples 1-8, 10 people in each group), and the hair essences of Examples 1-9, the hair essences of Comparative Examples 1-8 and the matrix essence were used as test samples respectively. The volunteers selected an appropriate amount of use, evenly applied the hair essence on the hair surface, used it once a day for a total of 30 days. Before and after using the samples, the combing work, friction coefficient and glossiness of the volunteers' hair were tested, the values of the combing work, friction coefficient and glossiness of the hair before and after using the samples were recorded, and the corresponding change rates were calculated. The ability of the test samples to repair and nourish the hair was judged through the change rates.

[0119] Instruments used for testing: The multi-functional hair testing system (MTT175) was used to test the combing work and friction coefficient of the hair; the Glossymeter GL2000 was used to test the glossiness of the hair.

[0120] Change rate of hair combing work (%) = (Combing work of hair after using the product - Combing work of hair before using the product) / Combing work of hair before using the product * 100%. The combing work of the hair refers to the resistance encountered when the comb passes through the hair. The smaller the combing work of the hair, the better the combability of the hair; therefore, when the change rate of the hair combing work is negative, it indicates that after using the product, the combability of the hair becomes better, and the repair and nourishment effects of the product on the hair are better.

[0121] Change rate of hair friction coefficient (%) = (Friction coefficient of hair after using the product - Friction coefficient of hair before using the product) / Friction coefficient of hair before using the product * 100%. The friction coefficient can reflect the condition of the outermost layer of the hair and provide information on the damage and smoothness of the hair surface. The smaller the friction coefficient, the smaller the stiffness of the hair, indicating that the hair is soft and smooth. The smaller the hair friction coefficient, the lower the damage of the hair surface and the smoother the hair; therefore, when the change rate of the hair friction coefficient is negative, it indicates that after using the product, the damage of the hair surface is improved and the hair is smoother after using the product.

[0122] Change rate of hair glossiness (%) = (Glossiness of hair after using the product - Glossiness of hair before using the product) / Glossiness of hair before using the product * 100%. Healthy hair can often emit a natural gloss. The greater the change rate of the hair glossiness, the better the hair care, repair, nourishment and other effects of the product.

[0123] The test results of the hair repair and nourishment effects are shown in Table 5.

[0124] Table 5: Test results of hair repair and nourishment effects

[0125]

[0126]

[0127] As shown in the results of Table 5, the anti-hair-breakage effects of Examples 1 to 9 are better than those of Comparative Examples 1 to 8, indicating that during the fermentation process, the types and ratios of fermentation raw materials, the types of carbon sources and nitrogen sources, and the types of fermentation strains and their addition ratios have a great influence on the hair repair and nourishing effects of the finally prepared composition. The hair repair and nourishing effects of Example 1 are better than those of Comparative Examples 1 to 4, indicating that there is a synergistic effect between white birch bark and Myroxylon balsamum leaves / stems during the fermentation process, and the hair repair and nourishing effects of the composition obtained by co-fermenting the two are the best. Replacing white birch bark with white birch leaves or replacing Myroxylon balsamum leaves / stems with Myroxylon balsamum roots results in poorer hair repair and nourishing effects of the prepared composition. The hair repair and nourishing effects of Example 1 are better than those of Comparative Examples 5 to 7, indicating that the types of bacteria have a great influence on the fermentation process. In the present invention, Lactococcus lactis subsp. lactis and Lactobacillus johnsonii are selected for symbiotic fermentation, and the hair repair and nourishing effects of the obtained composition are the best. The hair repair and nourishing effects of Example 1 are better than those of Examples 6 and 7 and Comparative Example 8, indicating that the inoculation ratio of Lactococcus lactis subsp. lactis and Lactobacillus johnsonii also has a great influence on the fermentation process. In the present invention, the inoculation ratio of the Lactococcus lactis subsp. lactis seed liquid and the Lactobacillus johnsonii seed liquid should be 1 to 4:1, more preferably 2:1. The hair repair and nourishing effects of Example 1 are better than those of Examples 2 and 3. In the present invention, the mass ratio of white birch bark powder to Myroxylon balsamum powder should be 3 to 7:1, more preferably 5:1. The hair repair and nourishing effects of Example 1 are better than those of Examples 4 and 5, indicating that the selection of carbon sources and nitrogen sources in the fermentation matrix affects the fermentation effect, and thus affects the hair repair and nourishing effects of the composition; in the present invention, the carbon source should be selected from at least one of glucose, sucrose, fructose syrup, and maltose, more preferably maltose; the nitrogen source should be selected from at least one of corn steep liquor, soybean cake powder, peanut cake powder, and yeast extract, more preferably soybean cake powder.

[0128] Test Example 4. Evaluation by Volunteers

[0129] 180 volunteers with damaged hair quality were selected (10 people in each group for the matrix essence, hair essence of Examples 1 to 9 and Comparative Examples 1 to 8), and the hair essence and matrix essence prepared from the compositions of Examples 1 to 9 and Comparative Examples 1 to 8 were used as test samples respectively. The volunteers selected an appropriate amount of use, evenly applied the hair essence on the hair strands, used it once a day for a total of 30 days. After using the samples, the feedback results of the volunteers' evaluation of the product efficacy were collected, and the results are shown in Table 6.

[0130] Table 6: Results of Volunteer Use Evaluation

[0131]

[0132]

[0133]

[0134] As shown in the results of Table 6, the evaluation results of the volunteers in Examples 1-9 were better than those in Comparative Examples 1-8, indicating that during the fermentation process, the types and ratios of the fermentation raw materials, the types of carbon sources and nitrogen sources, the types of fermentation strains and their addition ratios had a great impact on the usage effects of the finally prepared compositions. The effects of making the hair stronger, more shiny, smoother, the product having a repair effect, and the product having a nourishing effect in Example 1 were better than those in Comparative Examples 1-4, indicating that there was a synergistic effect between the white birch bark and the leaves / stems of Myroxylon balsamum var. pereirae during the fermentation process, and the usage effect of the composition obtained by co-fermenting the two was the best. Replacing the white birch bark with white birch leaves or replacing the leaves / stems of Myroxylon balsamum var. pereirae with the roots of Myroxylon balsamum var. pereirae resulted in worse evaluation results for the prepared compositions. The evaluation results of the volunteers in Example 1 were better than those in Comparative Examples 5-7, indicating that the type of bacteria had a great impact on the fermentation process. In the present invention, the symbiotic fermentation of Lactococcus lactis subsp. lactis and Lactobacillus johnsonii was selected, and the evaluation results of the volunteers for the obtained composition were the best. The evaluation results of the volunteers in Example 1 were better than those in Examples 6, 7 and Comparative Example 8, indicating that the inoculation ratio of Lactococcus lactis subsp. lactis and Lactobacillus johnsonii also had a great impact on the fermentation process. In the present invention, the inoculation ratio of the Lactococcus lactis subsp. lactis seed liquid and the Lactobacillus johnsonii seed liquid should be 1-4:1, more preferably 2:1. The evaluation results of the volunteers in Example 1 were better than those in Examples 2 and 3. In the present invention, the mass ratio of the white birch bark powder to the Myroxylon balsamum var. pereirae powder should be 3-7:1, more preferably 5:1. The evaluation results of the volunteers in Example 1 were better than those in Examples 4 and 5, indicating that the selection of carbon sources and nitrogen sources in the fermentation matrix affected the fermentation effect, and thus affected the usage effect of the composition and the evaluation results of the volunteers.

Claims

1. A method for preparing a composition with the effects of preventing hair breakage, repairing and nourishing, characterized in that, It includes the following steps: S1 Preparation of plant fermentation substrate: Mix the fermentation raw materials, carbon source, nitrogen source, inorganic salts and water evenly, sterilize at high pressure and high temperature, and then cool down to obtain the plant fermentation substrate; S2 Preparation of mixed fermentation broth: Inoculate the Lactococcus lactis subsp. lactis seed liquid and the Lactobacillus johnsonii seed liquid into the plant fermentation substrate described in step S1, and carry out fermentation to obtain the mixed fermentation broth; S3 Refining process: Inactivate and filter the mixed fermentation broth described in step S2 to obtain a composition with the effects of preventing hair breakage, repairing and nourishing; Among them, the fermentation raw materials described in step S1 include white birch bark powder and Myrothamnus flabellifolius powder; The Myrothamnus flabellifolius powder is Myrothamnus flabellifolius leaf / stem powder; The total addition amount of the white birch bark powder and the Myrothamnus flabellifolius powder is 0.5-2.0% of the mass of the plant fermentation substrate, and the mass ratio of the white birch bark powder to the Myrothamnus flabellifolius powder is 3-7:1; The total inoculation amount of the Lactococcus lactis subsp. lactis seed liquid and the Lactobacillus johnsonii seed liquid described in step S2 is 1%-3% of the mass of the plant fermentation substrate, and the mass ratio of the inoculation amounts of the Lactococcus lactis subsp. lactis seed liquid and the Lactobacillus johnsonii seed liquid is 1-4:1; The specific conditions of the fermentation described in step S2 are: fermentation temperature 35-42°C, natural pH, rotation speed 100-250 rpm, and fermentation time 36-72 h.

2. The preparation method of the composition with the effects of preventing hair breakage, repairing and nourishing as claimed in claim 1, characterized in that, The carbon source described in step S1 is selected from at least one of glucose, sucrose, fructose syrup, and maltose; the addition amount of the carbon source is 0.5-2% of the mass of the plant fermentation substrate; The nitrogen source is selected from at least one of corn steep liquor, soybean cake powder, peanut cake powder, and yeast extract; the addition amount of the nitrogen source is 0.5-2% of the mass of the plant fermentation substrate; The inorganic salts are selected from at least one of NaCl, Na2HPO4, NaH2PO4, KCl, K2HPO4, and KH2PO4; the addition amount of the inorganic salts is 0.05-0.5% of the mass of the plant fermentation substrate.

3. The preparation method of the composition with the effects of preventing hair breakage, repairing and nourishing as claimed in claim 1, characterized in that, The preparation method of the Lactococcus lactis subsp. lactis seed liquid described in step S2 is as follows: pick the Lactococcus lactis subsp. lactis colony with an inoculation loop and transfer it into a liquid medium, and culture it at 30-42 °C and 100-300 rpm for 24-72 h to obtain a Lactococcus lactis subsp. lactis seed liquid with a bacterial concentration of 1×10 7 ~1×10 9 cfu / ml; The preparation method of the Lactobacillus johnsonii seed liquid described in step S2 is as follows: pick a Lactobacillus johnsonii colony with an inoculation loop and transfer it into the liquid medium, and culture it at 30-42 °C and 100-300 rpm for 24-72 h to obtain a Lactobacillus johnsonii seed liquid with a bacterial concentration of 1×10 7 ~1×10 9 cfu / ml.

4. The preparation method of the composition with anti-breakage, repair and nourishing effects as claimed in claim 1, characterized in that, The temperature of the inactivation treatment described in step S3 is 85-115°C, and the time of the inactivation treatment is 15-30 min.

5. A composition with the effects of preventing hair breakage, repairing and nourishing prepared by the preparation method of the composition with the effects of preventing hair breakage, repairing and nourishing according to any one of claims 1-4.

6. Use of the composition with the effects of preventing hair breakage, repairing and nourishing according to claim 5 in the preparation of hair care products.

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