Use of a suspension composition containing dichlofenthion for controlling leafmining flies

Abstract

The present application relates to the technical field of pesticides, and discloses application of a suspension composition containing cyromazine to the prevention and treatment of liriomyza trifolii. The suspension composition is composed of cyromazine, deltamethrin and an auxiliary agent, and is prepared into a suspension agent, wherein the weight ratio of cyromazine to deltamethrin is 10:1-14:1. The suspension composition of the present application fills the market gap of the currently available pesticide without mixed composition for the prevention and treatment of liriomyza trifolii, has a good prevention and treatment effect on liriomyza trifolii, delays drug resistance, reduces the use amount of pesticides, reduces the use cost of farmers, is conducive to environmental protection and food safety, and is environmentally friendly.

Description

Technical Field

[0001] This invention belongs to the field of pesticide technology, specifically relating to the application of a suspension composition containing cyromazine for the control of onion leafminer flies. Background Technology

[0002] Cyromazine is a triazine insect growth regulator with specific activity against dipteran larvae. It can induce morphological abnormalities in dipteran larvae and pupae, resulting in incomplete or inhibited adult emergence. It is used to control flies in animal stables and to control agricultural pests such as the American serpentine leafminer on cucumbers, eggplants, green beans, leafy vegetables, and flowers.

[0003] Deltamethrin is the most toxic of the pyrethroid insecticides, with a toxicity to pests that is 100 times that of DDT, 80 times that of carbaryl, 550 times that of malathion, and 40 times that of parathion. It has both contact and stomach poison action; contact action is rapid and has a strong knockdown effect. It lacks fumigation and systemic action, but at high concentrations, it has a repellent effect on some pests. Its residual effect is long (7-12 days). When formulated as an emulsifiable concentrate or wettable powder, it is a moderately toxic insecticide. It has a broad insecticidal spectrum, effective against a variety of pests including Lepidoptera, Orthoptera, Thysanoptera, Hemiptera, Diptera, and Coleoptera, but its efficacy against mites, scale insects, and mirid bugs is very low or essentially ineffective. It may even stimulate mite reproduction; therefore, in cases of concurrent insect and mite infestations, it should be mixed with a specialized acaricide.

[0004] The onion leafminer (Liriomyza chinensis Kato) is a very important leafminer pest, mainly damaging plants of the genus Allium in the Liliaceae family, such as scallions, leeks, and onions, with scallions being the most severely affected. Both larvae and adults can cause damage, with larvae primarily feeding on the leaf mesophyll. After the mesophyll is consumed, only two layers of epidermis remain, exhibiting white linear streaks that form tunnels, severely impacting normal photosynthesis. Furthermore, the ovipositor of the female adult can pierce the leaves, providing a crucial pathway for pathogen invasion. Currently, control of this pest in production mainly relies on chemical insecticides. It is distributed in North and Northwest China. In southern Liaoning Province and North China, there are 4-5 generations per year; in Jiangxi Province, 12-13 generations; in Fujian Province, 13-15 generations; and in Guangdong Province, 18 generations, with overlapping generations. It overwinters or oversummers as pupae. Adults are active, flying among onion plants or resting at the tips of leaf sheaths. The larvae can move freely in and out of the tunnels in the leaf tissue and migrate to infest parts inside and outside the leaf sheath.

[0005] There are very few existing products for controlling onion leafminer, mainly cyromazine wettable powder and cyantraniliprole oil dispersible suspension. Because of the limited number of products available, pests are prone to developing severe resistance. Furthermore, the overlapping generations during pest outbreaks lead to farmers using increasingly larger amounts of pesticides. Therefore, providing a pesticide with good control effects, especially effective against resistant pests, is an urgent technical problem that needs to be solved in this field.

[0006] Through continuous innovation, the applicant has combined two agents with completely different mechanisms of action, enabling them to work synergistically against pests to achieve effective pest control, especially against resistant pests, offering both rapid and sustained efficacy. Furthermore, this invention can reduce residue levels and shorten the safety interval, making it safer for consumers. Therefore, the combination of cyromazine and deltamethrin is worthy of widespread application in agricultural production. Summary of the Invention

[0007] The purpose of this invention is to provide a suspension composition containing cyromazine for the control of onion leafminer, which can effectively control onion leafminer, has both rapid and sustained effects, has a good control effect on resistant pests, reduces residue and shortens the safety interval.

[0008] The technical solution of this invention is:

[0009] An application of a suspension composition containing cyromazine for the control of onion leafminer flies, characterized in that: the suspension composition is composed of cyromazine, deltamethrin, and adjuvants, and is prepared as a suspension, wherein the weight ratio of cyromazine to deltamethrin is 10:1 to 14:1, and the preferred weight ratio is 37:3.

[0010] Furthermore, the effective active ingredient content of the suspension composition is 110-600 g / L, preferably 400 g / L.

[0011] Furthermore, the additives are selected from one or more of dispersants, wetting agents, antifreeze agents, preservatives, defoamers, thickeners, and water.

[0012] Furthermore, the dosage of the active ingredient in the suspension composition is 60–120 g / ha, with a preferred dosage of 90–120 g / ha.

[0013] Furthermore, the water consumption for using the suspension composition to control onion leafminer is 450–750 liters / ha, with a preferred water consumption of 600–750 liters / ha.

[0014] When the suspension composition is prepared into a suspension, it includes the following components and contents in percentage: 100-560 g / L of cyromazine, 7-55 g / L of deltamethrin, 10-100 g / L of dispersant, 10-100 g / L of wetting agent, 1-20 g / L of defoamer, 2-30 g / L of thickener, 0-100 g / L of antifreeze, and water as the balance.

[0015] Compared with the prior art, the composition of the present invention has the following beneficial effects: (1) Compared with single agents, the composition has a significant synergistic effect on onion leafminer, and significantly improves the control effect; (2) It is low in toxicity and high in efficiency, reduces the amount of pesticides used, reduces the amount of pesticide residues on crops, and has a short safety interval; (3) It has both fast-acting and long-acting effects; (4) It delays the development of pesticide resistance in pests. Detailed Implementation

[0016] The present invention will be further described below with reference to embodiments, but the present invention is not limited thereto.

[0017] Application Example 1

[0018] Example 1: 400 g / L cyromazine·deltamethrin suspension

[0019] A 400 g / L cyromazine·deltamethrin suspension was prepared by adding 389.47 g of 95% cyromazine technical grade, 30.61 g of 98% deltamethrin technical grade, 15 g of alkyl naphthalene sulfonate formaldehyde polymer, 15 g of polycarboxylate, 10 g of fatty alcohol polyoxyethylene ether, 10 g of alkyl glycoside, 30 g of ethylene glycol, 1.5 g of xanthate gum, 2 g of magnesium aluminum silicate, 2 g of organosilicon, 5 g of sodium benzoate, and water to a final volume of 1000 g.

[0020] Example 2: 400 g / L cyromazine·deltamethrin suspension

[0021] A 400 g / L cyromazine·deltamethrin suspension was prepared by adding 389.47 g of 95% cyromazine technical grade, 30.61 g of 98% deltamethrin technical grade, 20 g of alkyl naphthalene sulfonate, 20 g of trisiloxane polyoxyethylene ether, 40 g of propylene glycol, 3 g of carboxymethyl cellulose, 0.8 g of C8-10 fatty alcohol, 5 g of sodium benzoate, 5 g of silica, and water to a final volume of 1000 g.

[0022] Example 3: 110 g / L cyromazine·deltamethrin suspension

[0023] A 110 g / L cyromazine·deltamethrin suspension was prepared by adding 105.26 g of 95% cyromazine technical grade, 10.20 g of 98% deltamethrin technical grade, 20 g of polycarboxylate, 10 g of polyarylphenol polyoxyethylene ether, 50 g of propylene glycol, 2 g of carboxymethyl cellulose, 0.5 g of C10-20 saturated fatty acid compound, and water to a final volume of 1000 g.

[0024] Example 4: 600 g / L cyromazine·deltamethrin suspension

[0025] A 600 g / L cyromazine·deltamethrin suspension was prepared by adding 589.47 g of 95% cyromazine technical grade, 40.82 g of 98% deltamethrin technical grade, 20 g of lignin sulfonate, 10 g of EO-PO block copolymer, 20 g of castor oil polyoxyethylene ether, 40 g of glycerol, 1 g of magnesium aluminum silicate, 2 g of sodium benzoate, 3 g of organosilicone defoamer, and water to a final volume of 1000 g.

[0026] Implementation Example 2:

[0027] Indoor combined toxicity assay of cyromazine, deltamethrin and mixed products against onion leafminer

[0028] 1. Experimental Objective

[0029] The toxicity of cyromazine and deltamethrin, and their different mixtures, to onion leafminer was determined in the laboratory, and their synergistic effect was evaluated to clarify their compatibility and provide a scientific basis for the research and development of cyromazine and deltamethrin mixtures.

[0030] 2 Experimental conditions

[0031] 2.1 Test Target

[0032] Adult leek leafminer (Liriomyza chinensis Kato) were collected from leek leaves grown in Tianzhuang, Fanzhen Town, Tai'an City, Shandong Province. After collection, they were bred indoors using leek seedlings.

[0033] 2.2 Cultivation Conditions

[0034] Fresh, clean onion shoots (not previously consumed by other leafminers) were placed in well-sealed mesh rearing cages. Onion leafminers that had emerged that day were separated into male-to-female ratios of approximately 1:1, with about 20 pairs of adult females placed in each cage. After 4 hours, the flies were removed (to ensure uniformity in larval instars after hatching). Rearing conditions included indoor temperature (28±1℃), light intensity (light-to-dark ratio 16:8), and humidity (60%–70%). On the 5th day after introducing the adults, onion tubes containing healthy, uniform second-instar larvae (tunnels longer than 2 cm) were selected for toxicity testing.

[0035] 3 Experimental Design

[0036] 3.1 Test reagents

[0037] Cyromazine 95% technical grade; deltamethrin 98.5% technical grade.

[0038] 3.2 Reagent Preparation

[0039] A stock solution of 1.0 × 10⁴ mg / L was prepared using dimethyl sulfoxide as a solvent for the test agents cyromazine and deltamethrin technical, and stored in a refrigerator for later use. Based on the preliminary test, the stock solution was further diluted with an aqueous solution containing 0.1% Tween 80, with five concentrations designed for each treatment.

[0040] Based on the preliminary test, the effective component ratios of the two mixtures were 8:1, 10:1, 37:3, 14:1, and 16:1, and 1.0 × 10⁴ mg / L stock solutions were prepared and stored in a refrigerator for later use. The stock solutions were then diluted with an aqueous solution containing 0.1% Tween 80. Five concentrations were designed for each treatment, and 120 second-instar (day 5, burrows longer than 2 cm) larvae of the onion leafminer were treated at each concentration. The mixtures were divided into four replicates, with an equal volume of dimethyl sulfoxide and 0.1% Tween 80 aqueous solution without the pesticide serving as blank controls.

[0041] Cyromazine (A): 120, 60, 30, 15, 7.5 mg / L

[0042] Deltamethrin (B): 12, 6, 3, 1.5, 0.75 mg / L

[0043] A:B(8:1): 56, 28, 14, 7, 3.5mg / L

[0044] A:B(10:1): 60, 30, 15, 7.5, 3.75mg / L

[0045] A:B(37:3): 64, 32, 16, 8, 4mg / L

[0046] A:B(14:1): 72, 36, 18, 9, 4.5mg / L

[0047] A:B(16:1): 80, 40, 20, 10, 5mg / L

[0048] Each concentration of the test agent was treated four times, with each replicate containing no fewer than 30 second-instar (burrowing tunnels longer than 2 cm) larvae of the onion leafminer. An equal volume of dimethyl sulfoxide and 0.1% Tween 80 aqueous solution without the agent served as blank controls.

[0049] 4. Test Methods

[0050] Leaf dipping method. This method is based on and modified from the People's Republic of China agricultural industry standards "Guidelines for Indoor Bioassay Tests of Pesticides - Insecticides" Part 7: Determination of Combined Effects of Mixtures (NY / T1154.7-2006) and Part 14: Leaf Dipping Method (NY / T 1154.14-2008).

[0051] Pick up the leaf attached with the 2nd instar larvae of Liriomyza chinensis with tweezers, dip it in the prepared liquid medicine for 10 s, then take it out and let it dry naturally, and mark 30 mines. When dipping, first dip the control, and then proceed in order from low concentration to high concentration. Then cover each treated leaf with a fresh-keeping bag with a large number of breathing holes pricked by a needle, clamp the mouth with a small clip, and place it in an artificial constant-temperature insect rearing room with a temperature of (28±1°C), a light-dark ratio of 16:8, and a humidity of 60%-70% for rearing.

[0052] 5 Data Investigation and Statistical Analysis

[0053] 5.1 Investigation Time and Method

[0054] Check the pupation numbers of each treatment 48 h after deltamethrin treatment. Check the pupation numbers of each treatment 96 h after cyromazine and mixture treatments. Larval death criterion: Those that did not successfully pupate were considered dead.

[0055] 5.2 Data Statistical Analysis

[0056] If the mortality of the blank control is less than 5%, no correction is needed; if the mortality of the blank control is between 5% and 20%, calculate the corrected mortality of each treatment using formula (2); if the mortality of the blank control is above 20%, the experiment needs to be repeated.

[0057] Use DPS data processing software to calculate the experimental results, respectively obtain the toxicity regression equations, LC50, LC90 and 95% confidence limits of the two single agents and five ratios of the experimental agents, and record the original data of all replicates of each treatment.

[0058] Calculate the co-toxicity coefficient (CTC) according to the Sun Yunpei method, and evaluate the synergistic effect of the mixture of agents based on the co-toxicity coefficient (CTC), that is, CTC≤80 is antagonistic effect, 80<CTC<120 is additive effect, and CTC≥120 is synergistic effect. The corrected mortality is calculated according to formulas (1) and (2) respectively. The co-toxicity coefficient (CTC) is calculated according to formulas (3), (4), (5).

[0059] Formula (1): P=(K / N)×100

[0060] In the formula: P is the mortality rate (%);

[0061] K is the number of dead insects (heads);

[0062] N is the total number of treated insects (heads).

[0063] Formula (2): P1=[(P t -P0) / (1-P0)]×100 In the formula: P1 is the corrected mortality rate (%);

[0064] P t is the treatment mortality rate (%).

[0065] P0 represents the mortality rate (%) in the blank treatment.

[0066] Formula (3): ATI = (S / M) × 100

[0067] Where: ATI is the measured toxicity index of the single test dose;

[0068] S is the LC50 of the standard reagent. 50 (mg / L);

[0069] M is the LC50 of the test mixture. 50 (mg / L).

[0070] Formula (4): TTI = TI A ×P A +TI B ×P B

[0071] In the formula: TTI is the theoretical toxicity index of the mixture;

[0072] TI A The toxicity index of drug A;

[0073] P A The percentage (%) of agent A in the mixture;

[0074] TI B The toxicity index of drug B;

[0075] P B The percentage (%) of agent B in the mixture.

[0076] Formula (5): CTC=(ATI / TTI)×100

[0077] In the formula: CTC is the co-toxicity coefficient;

[0078] ATI is the measured toxicity index of the mixture;

[0079] TTI is the theoretical toxicity index of a mixture.

[0080] 5. Experimental Results

[0081] Table 1. Toxicity test results of the mixture of cyromazine and deltamethrin on the leafminer fly.

[0082]

[0083] As shown in Table 1 of the experimental results, the tested pesticide, cyromazine, has good toxicity to second-instar larvae of the onion leafminer fly, with an LC50 value of [missing information]. 50 The value was 33.0451 mg / L, LC. 90The value was 302.1933 mg / L; deltamethrin also has high toxicity, LC50. 50 The value was 3.2283 mg / L, LC. 90 The value was 26.9820 mg / L; the toxicity (LC50) of different ratios of the two agents to the second instar larvae of the onion leafminer was measured. 50 The concentrations of cyromazine and deltamethrin were 14.4759, 14.9420, 16.2129, 17.0106, and 20.5406 mg / L, respectively, with co-toxicity coefficients (CTC) reaching 112.66, 120.22, 120.41, 120.23, and 104.24, respectively. This indicates that when the experimental design ratios of cyromazine and deltamethrin are 10:1-14:1, the CTC is greater than 120, showing a significant synergistic effect. The synergistic effect is most significant at a ratio of 37:3.

[0084] Application Example 3: Field Efficacy Trial for Controlling Onion Leafminer (Example 1)

[0085] 1. Experimental Objective

[0086] To verify the efficacy and safety of the 400 g / L deltamethrin suspension concentrate developed by the applicant against onion leafminer, to clarify the field dosage and application technology, and to provide a scientific basis for pesticide registration.

[0087] 2. Test Basis

[0088] "Good Manufacturing Practice for Pesticide Registration Trials", "GB / T 17980.66-2004 Guidelines for Field Efficacy Trials of Pesticides (II) Insecticides for the Control of Vegetable Leafminer", and "NWP-SOP-I-016 Operating Procedures for Field Trials of Vegetable Leafminer".

[0089] 3 Experimental Locations

[0090] This experiment was conducted in Yangling District, Shaanxi Province.

[0091] 4. Selection of test subjects, crops and varieties

[0092] Experimental subject: Onion leafminer (Liriomyza chinensis Kato);

[0093] The experimental crop was scallions, specifically the Jin Chang No. 3 variety, which grew well.

[0094] 5. Experimental Design and Arrangement

[0095] 5.1 Dosage and Numbering of Pharmaceuticals

[0096] Table 2 Experimental Design of Test Reagents

[0097]

[0098] 5.2 Application time and frequency

[0099] In the early stages of the scallion leafminer infestation, use a manual backpack sprayer with a working pressure of 0.3-0.4 MPa and a nozzle diameter of 0.7 mm. Apply the pesticide once on June 28, 2022.

[0100] 5.3 Usage Capacity

[0101] The amount of pesticide solution sprayed per mu is 50 liters, and the amount of pesticide solution sprayed per hectare is 750 liters.

[0102] 5.4 Survey Time and Frequency

[0103] The insect population was surveyed before the pesticide was applied, and the number of live insects was surveyed at 3, 7 and 10 days after the pesticide was applied, for a total of 4 times.

[0104] During the survey, select 10 plants in the middle of each plot. From each plant, select one leaf from the middle or upper part of the leaf, ensuring it has 3-5 insect tunnels (0.5-1 cm long) indicating early-stage larvae (at this stage, the larvae are around 1st or 2nd instar). Mark each tunnel with an oil-based marker about 1 cm to the side of the tunnel's tip, aligning it with the tunnel's tip. Leaves with empty tunnels should not be selected. When assessing control efficacy, live larvae are those with fresh, plump bodies and emergence holes, while dead larvae are those with shriveled or discolored bodies. If identification is difficult, live larvae can be identified by elongated tunnels, emergence holes, or newly formed tunnels.

[0105] Survey time and content:

[0106] Baseline survey, before processing;

[0107] An investigation was conducted 3 days after the application of the pesticide, and the number of empty insect passages, dead insects, and live insects were recorded.

[0108] A survey was conducted 7 days after application, and the same items were recorded as above.

[0109] A survey was conducted 10 days after the application of the pesticide, and the same items were recorded as above.

[0110] 5.5 Method for Calculating Drug Efficacy

[0111] The field efficacy calculation is based on the formula in the "Guidelines for Field Efficacy Trials of Pesticides" to calculate the insect population reduction rate and control effect.

[0112]

[0113]

[0114] 6 Experimental Results

[0115] Table 3. Results of field efficacy trial for controlling onion leafminer in Example 1.

[0116]

[0117] Example 1: Control of Onion Leafminer. Table 3 shows the results of an experiment using 400 g / L cyromazine·deltamethrin suspension to control onion leafminer. Table 3 shows that the control effects of six treatments (400 g / L cyromazine·deltamethrin suspension at 60 g / ha, 90 g / ha, and 120 g / ha) and the control agents (70% cyromazine wettable powder at 220.5 g / ha, 25 g / L deltamethrin EC at 11.25 g / ha, and 10% cypermethrin dispersible oil suspension at 28.5 g / ha) 3 days after application were... The efficacy rates were 61.32%, 69.36%, 72.52%, 65.22%, 70.51%, and 64.99%, respectively; the efficacy rates 7 days after application were 78.49%, 86.52%, 89.38%, 82.59%, 82.42%, and 80.16%, respectively; and the efficacy rates 10 days after application were 88.28%, 93.33%, 95.76%, 88.30%, 82.50%, and 87.87%, respectively.

[0118] Analysis of variance showed that at 3 and 7 days post-application, the control efficacy of the six treatments—400 g / L cyromazine·deltamethrin suspension at 60 g(ai) / ha, 90 g(ai) / ha, and 120 g(ai) / ha, and the control treatments—70% cyromazine wettable powder at 220.5 g(ai) / ha, 25 g / L deltamethrin emulsifiable concentrate at 11.25 g(ai) / ha, and 10% cypermethrin dispersible oil suspension at 28.5 g(ai) / ha—did differ significantly at the 0.05 level. At 10 days post-application, the control efficacy of each treatment differed significantly at the 0.05 and 0.01 levels.

[0119] The DMRT test results showed that, 3 days after application, at the 0.05 level, there was no significant difference in the control efficacy between the 400 g / L cyromazine·deltamethrin suspension at 60 g(ai) / ha and the control treatments of 70% cyromazine wettable powder at 220.5 g(ai) / ha and 10% cypermethrin dispersible oil suspension at 28.5 g(ai) / ha. Both treatments were less effective than the 400 g / L cyromazine·deltamethrin suspension at 120 g(ai) / ha.

[0120] Seven days after application, at the 0.05 level, there was no significant difference in the control efficacy of the 400 g / L cyromazine·deltamethrin suspension at 60 g(ai) / ha compared to the control treatments of 70% cyromazine wettable powder at 220.5 g(ai) / ha, 25 g / L deltamethrin emulsifiable concentrate at 11.25 g(ai) / ha, and 10% cypermethrin dispersible oil suspension at 28.5 g(ai) / ha. All of these treatments were less effective than the 400 g / L cyromazine·deltamethrin suspension at 120 g(ai) / ha.

[0121] Ten days after application, at the 0.05 level, there was no significant difference in control efficacy between treatments of 400 g / L cyromazine·deltamethrin suspension at 90 g(ai) / ha and 120 g(ai) / ha. Both treatments were superior to those of 400 g / L cyromazine·deltamethrin suspension at 60 g(ai) / ha and the control treatments of 70% cyromazine wettable powder at 220.5 g(ai) / ha, 25 g / L deltamethrin emulsifiable concentrate at 11.25 g(ai) / ha, and 10% cypermethrin dispersible oil suspension at 28.5 g(ai) / ha. Furthermore, at the 0.05 level, the control efficacy of 400 g / L cyromazine·deltamethrin suspension at 60 g(ai) / ha was comparable to that of the control treatment of 70% cyromazine wettable powder at 220.5 g(ai) / ha. There was no significant difference in control efficacy between the treatments of 10% cypermethrin·deltamethrin suspension at 28.5 g(ai) / ha and 25 g / L deltamethrin emulsifiable concentrate at 11.25 g(ai) / ha; both were superior to the treatment of 25 g / L deltamethrin emulsifiable concentrate at 11.25 g(ai) / ha. At the 0.01 level, there was no significant difference in control efficacy between the treatments of 400 g / L cypermethrin·deltamethrin suspension at 60 g(ai) / ha and the control treatments of 70% cypermethrin wettable powder at 220.5 g(ai) / ha, 25 g / L deltamethrin emulsifiable concentrate at 11.25 g(ai) / ha, and 10% cypermethrin·deltamethrin suspension at 28.5 g(ai) / ha; all were inferior to the treatment of 400 g / L cypermethrin·deltamethrin suspension at 120 g(ai) / ha.

[0122] In summary, 400 g / L cyromazine·deltamethrin suspension concentrate has a good control effect on onion leafminer. Considering the differences in dosage after application, the recommended dosage is 90 g(ai) / ha to 120 g(ai) / ha. Application should be performed at the early stage of onion leafminer infestation.

[0123] Application Example 4: Field Efficacy Trial for Controlling Onion Leafminer (Example 2)

[0124] The experimental objectives, experimental basis, and efficacy calculation methods are the same as in Application Example 3.

[0125] 1. Experimental Location

[0126] This experiment was conducted in Meichuan Town, Wuxue City, Hubei Province.

[0127] 2. Selection of test subjects, crops and varieties

[0128] Test subject: Onion leafminer;

[0129] Experimental crop: Scallion, specifically the Four Seasons Scallion variety.

[0130] 3. Application time and frequency

[0131] On June 12, 2020, the medication was administered once.

[0132] 4 Usage Capacity

[0133] The amount of pesticide solution sprayed per mu is 45 liters, and the amount of pesticide solution sprayed per hectare is 675 liters.

[0134] 5. Dosage and Number of Drugs

[0135] Table 4 Experimental Design of Test Reagents

[0136]

[0137] 6. Survey Time, Frequency, and Methods

[0138] A baseline population was surveyed before application, and efficacy surveys were conducted 3, 7, and 10 days after application. A total of 4 surveys were conducted.

[0139] Ten plants were surveyed in the middle row of each plot. On each plant, 3-5 larval tunnels (0.5-1 cm long) in the early stages of growth were selected (at this stage, the larvae are around 1st-2nd instar). One point was marked approximately 1 cm to each side of the front end of each tunnel using an oil-based marker, aligning it with the front end of the tunnel. When assessing control efficacy, larvae with fresh, plump bodies and emergence holes were considered live.

[0140] 7 Experimental Results

[0141] Table 5. Control efficacy of Example 2 against onion leafminer - insect population reduction rate

[0142]

[0143] Table 6. Control efficacy of Example 2 against onion leafminer - Control effect

[0144]

[0145] Note: Lowercase letters indicate differences between treatments at the 0.05 level, and uppercase letters indicate differences between treatments at the 0.01 level.

[0146] In Example 2, the test compound 400 g / L cyromazine·deltamethrin suspension showed excellent control efficacy against the onion leafminer. Three days after application, the population reduction rate of the onion leafminer was between 70.63% and 80.51% and the control efficacy was between 71.54% and 81.10% for treatments of 10 mL / mu, 15 mL / mu, and 20 mL / mu of the test compound cyromazine·deltamethrin suspension. The control efficiencies of the control compounds 70% cyromazine wettable powder (21 g / mu), 25 g / L deltamethrin emulsifiable concentrate (30 mL / mu), and 10% cypermethrin dispersible oil suspension (24 mL / mu) against the onion leafminer were 65.63%, 71.09%, and 71.97%, respectively. Seven days after application, the control efficacy of the test substance 400 g / L cyromazine·deltamethrin suspension at various doses against the onion leafminer was higher than that three days after application, with efficacy ranging from 85.46% to 93.65%. The control efficacies of the control substances 70% cyromazine wettable powder at 21 g / mu, 25 g / L deltamethrin emulsifiable concentrate at 30 mL / mu, and 10% cypermethrin dispersible oil suspension at 24 mL / mu against the onion leafminer were 83.61%, 83.45%, and 85.58%, respectively.

[0147] The test compound 400 g / L cyromazine·deltamethrin suspension showed excellent control efficacy against leafminer flies. Ten days after application, the control rates of leafminer flies on onions were 87.58%-94.64% for treatments of 10 mL / mu, 15 mL / mu, and 20 mL / mu for cyromazine·deltamethrin suspension, and the control efficacy was 88.84%-95.19%. The control efficiencies of the control compounds 70% cyromazine wettable powder (21 mL / mu), 25 g / L deltamethrin emulsifiable concentrate (30 mL / mu), and 10% cypermethrin dispersible oil suspension (24 mL / mu) against leafminer flies on onions were 86.89%, 85.74%, and 87.03%, respectively.

[0148] The Duncan multiple range method was used to analyze the significant differences in the control efficacy of each treatment 10 days after application. The results showed that there was a significant difference in the control efficacy of the test agent 400 g / L cyromazine·deltamethrin suspension at 10 mL / mu and 20 mL / mu against the leafminer of the onion. The control efficacy of the test agent 400 g / L cyromazine·deltamethrin suspension at 15 mL / mu was significantly different from that of the control agents 70% cyromazine wettable powder at 21 mL / mu, 25 g / L deltamethrin emulsifiable concentrate at 30 mL / mu, and 10% cypermethrin dispersible oil suspension at 24 mL / mu, indicating that the test agent was more effective.

[0149] The above description, in conjunction with specific preferred embodiments, provides a further detailed explanation of the present invention. It should not be construed that the specific implementation of the present invention is limited to these descriptions. For those skilled in the art, various simple deductions or substitutions can be made without departing from the concept of the present invention, and all such modifications and substitutions should be considered within the scope of protection of the present invention.

Claims

1. An application of a suspension composition containing cyromazine for controlling onion leafminer flies, characterized in that: The suspension composition consists of cyromazine, deltamethrin, and adjuvants, and is made into a suspension, wherein the weight ratio of cyromazine to deltamethrin is 10:1 to 14:

1.

2. The application according to claim 1, characterized in that: The weight ratio of cyromazine to deltamethrin is 37:

3.

3. The application according to claim 1 or 2, characterized in that: The total content of cyromazine and deltamethrin in the suspension composition is 110-600 g / L.

4. The application according to claim 3, characterized in that: The total content of cyromazine and deltamethrin in the suspension composition is 400 g / L.

5. The application according to claim 1, characterized in that: The additives are selected from one or more of dispersants, wetting agents, antifreeze agents, preservatives, defoamers, thickeners, and water.

6. The application according to claim 5, characterized in that: The dosage of the active ingredient in the suspension composition is 60-120 grams per hectare.

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