Composition with hair follicle protecting effect, preparation method and application

Abstract

The present invention discloses a composition having a hair follicle protective effect, a preparation method, and an application thereof. The raw materials of the composition include 1 to 10 parts by weight of a hair follicle care agent, 0.5 to 5 parts by weight of a moisturizer, 1 to 15 parts by weight of an emulsifier, 0.5 to 10 parts by weight of an emollient, a pH adjuster, and 0.01 to 1000 parts by weight of water. The hair follicle care agent includes at least one of hydroxytyrosol and oleuropein, and the pH of the composition is 6.0 to 7.0. The composition of the present invention uses hydroxytyrosol and oleuropein as the main active ingredients and has unique protective and therapeutic effects on the skin, especially hair follicles. It can effectively inhibit the occurrence of oxidative stress, thereby reducing the occurrence of various skin diseases, and can also prevent hair loss.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to a composition having a hair follicle protecting effect, a preparation method and an application thereof. Background Art

[0002] Oxidative stress (OS) is usually manifested as an imbalance in the generation and clearance of reactive oxygen species (ROS) in the body, which leads to a state in which ROS levels in the body tend to increase. This can cause damage to the body's macromolecular proteins and decreased cell viability, especially for the skin, and is very likely to cause neurogenic inflammation.

[0003] Hair follicles are regenerative micro-organs primarily composed of dermal papilla cells (DPCs) that function to grow hair. Therefore, when hair follicles, as skin appendages, are stimulated by OS, the primary symptom is hair loss. In fact, histological findings show decreased pigmentation and increased ROS production around gray hair, demonstrating a close relationship between OS and hair follicle physiology and pathology. Studies have also shown that oxidative stress is one of the primary pathogenesis of androgenic alopecia. Furthermore, due to the specific structure of hair follicles, they are inextricably linked to other skin appendages, such as the sebaceous glands. Therefore, when hair follicles become abnormal due to OS stimulation, abnormalities in other skin appendages, such as the sebaceous glands, can also occur, leading to symptoms such as acne, papules, pustules, and nodules. Therefore, reducing the damage caused by OS stimulation is an important area of daily chemical care applications.

[0004] The existing daily chemical care products on the market mostly only provide auxiliary functions of superficial anti-inflammatory, and rarely involve the function of inhibiting OS stimulation. Various skin symptoms will still recur during use. Therefore, a daily chemical care product that can inhibit OS stimulation while playing an anti-inflammatory role in hair follicles, autophagy of metabolic waste, and achieve the purpose of multiple care of hair follicles is particularly necessary.

[0005] In view of this, the present invention is proposed. Summary of the Invention

[0006] The purpose of the present invention is to provide a composition with hair follicle protecting effect, a preparation method and application.

[0007] The present invention is achieved in that:

[0008] In a first aspect, the present invention provides a composition having a hair follicle protecting effect, the raw materials including 1 to 10 parts by weight of a hair follicle care agent, 0.5 to 5 parts by weight of a moisturizer, 1 to 15 parts by weight of an emulsifier, 0.5 to 10 parts by weight of an emollient, a pH adjuster and 0.01 to 1000 parts by weight of water, wherein the hair follicle care agent includes at least one of hydroxytyrosol and oleuropein, and the pH of the composition is 6.0 to 7.0.

[0009] In an optional embodiment, the mass fraction of water in the raw material is 50 wt% to 99 wt%. Preferably, the mass fraction of water in the raw material is 70 wt% to 99 wt%.

[0010] In an optional embodiment, the raw materials include 3 to 6 parts by weight of a hair follicle care agent, 0.5 to 3 parts by weight of a moisturizer, 1 to 10 parts by weight of an emulsifier, 1 to 5 parts by weight of an emollient, and 100 to 200 ml of water.

[0011] In an optional embodiment, the raw material satisfies at least one of the following items ①-⑤:

[0012] ① The hair follicle care agent is at least one of hydroxytyrosol and oleuropein;

[0013] ② The moisturizing agent includes at least one of sodium hyaluronate, arbutin, and glycerin;

[0014] ③ The emulsifier includes at least one of rhamnolipid and sophorolipid;

[0015] ④ The emollient includes at least one of squalane, squalene, and vitamin E;

[0016] ⑤ The pH regulator includes at least one of caffeic acid, protocatechuic acid, malic acid, citric acid, and lactic acid, or a strong base salt of at least one of them.

[0017] In a second aspect, the present invention provides a method for preparing the composition having a hair follicle protecting effect according to any one of the aforementioned embodiments, comprising:

[0018] Mixing: mixing the hair follicle care agent, moisturizer, emulsifier, emollient and water at 30° C. to 50° C. to obtain a mixed solution;

[0019] pH adjustment, and then adjusting the pH of the mixed solution to 6.0-7.0 using a pH adjuster to obtain the composition.

[0020] In an optional embodiment, the rotation speed of the mixing step is 500 r / min to 1000 r / min, and the mixing time is 30 min to 60 min.

[0021] In an optional embodiment, before adjusting the pH of the mixed solution, the mixed solution is homogenized for 10 to 20 minutes, the temperature of the homogenization step is 15° C. to 30° C., and the pressure of the homogenization step is 80 MPa to 110 MPa.

[0022] In an optional embodiment, the mixing step is to sequentially add the hair follicle care agent, emulsifier, moisturizer and emollient into water at 30° C. to 50° C. and mix them to obtain a mixed solution.

[0023] In a third aspect, the present invention provides a use of the composition with hair follicle protecting effect described in any one of the aforementioned embodiments in daily chemical products.

[0024] In an optional embodiment, the dosage form of the daily chemical product is any one of a cream, gel, patch, spray, ointment, plaster, emulsion, liniment, paste, mud pack, hair conditioner, hair essence, shampoo, hair cream, hair mask, hair soap, hair cleanser, hair oil, hair dyeing auxiliary agent and hair spray.

[0025] The present invention has the following beneficial effects:

[0026] (1) The composition of the present invention uses hydroxytyrosol and oleuropein as the main active ingredients. The synergistic effect of the components has a unique protective and therapeutic effect on the skin, especially the hair follicles, and can effectively inhibit the occurrence of oxidative stress, thereby reducing the occurrence of various skin diseases and preventing hair loss.

[0027] (2) The hydroxytyrosol in the composition of the present invention, also known as 3,4-dihydroxyphenylethanol, is a natural product with strong antioxidant effects. It also has good anti-inflammatory, antibacterial, and autophagy-promoting biological activities, and has not yet been found to cause toxic damage to the body. By utilizing the multiple biological activities of hydroxytyrosol itself, while inhibiting OS stimulation, it has the effects of anti-inflammatory and autophagy of metabolic waste on hair follicles, achieving the purpose of multi-care for hair follicles, and has a relatively outstanding preventive effect on various skin diseases, hair loss, etc.

[0028] (3) The ingredients of the composition of the present invention are all natural products, will not cause harm to the human body, and are green and environmentally friendly; and the ingredients are mild and non-irritating, and have a wide range of applications.

[0029] (4) No harmful substances are introduced during the preparation process of the composition of the present invention, and the production is green and the process is environmentally friendly; the preparation process is simple and the cost is low. DETAILED DESCRIPTION

[0030] To make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention are described clearly and completely below. Where specific conditions are not specified in the embodiments, conventional conditions or conditions recommended by the manufacturer are used. Where the manufacturer of the reagents or instruments is not specified, all are conventional products that can be purchased commercially.

[0031] One embodiment of the present application provides a composition with a hair follicle protecting effect, the raw materials including 1 to 10 parts by weight of a hair follicle care agent, 0.5 to 5 parts by weight of a moisturizer, 1 to 15 parts by weight of an emulsifier, 0.5 to 10 parts by weight of an emollient, a pH adjuster and 0.01 to 1000 parts by weight of water, wherein the hair follicle care agent includes at least one of hydroxytyrosol and oleuropein, and the pH of the composition is 6.0 to 7.0.

[0032] The main active ingredient in this embodiment is a hair follicle conditioning agent, which has unique protective and therapeutic effects on the skin, especially hair follicles. It can effectively inhibit the occurrence of oxidative stress, thereby reducing the occurrence of various skin diseases and preventing hair loss. Water in this embodiment serves as a diluent to prevent excessive concentrations of other components from irritating epidermal cells or hair follicles. The pH adjuster is used to adjust the pH value of the composition, and its dosage can be determined based on the acidity and alkalinity of the other components and their dosage.

[0033] In some optional embodiments, the mass fraction of water in the raw material is 50 wt% to 99 wt%. Preferably, the mass fraction of water in the raw material is 70 wt% to 99 wt%.

[0034] In some preferred embodiments, the raw materials include 3 to 6 parts by weight of a hair follicle care agent, 0.5 to 3 parts by weight of a moisturizer, 1 to 10 parts by weight of an emulsifier, 1 to 5 parts by weight of an emollient, and 100 to 200 ml of water.

[0035] In some optional embodiments, the hair follicle care agent is at least one of hydroxytyrosol and oleuropein, preferably with hydroxytyrosol as the main active ingredient, which has unique protective and therapeutic effects on the skin, especially hair follicles, and can effectively inhibit the occurrence of oxidative stress, thereby reducing the occurrence of various skin diseases, and can also prevent hair loss.

[0036] In some optional embodiments, the moisturizing agent includes at least one of sodium hyaluronate, arbutin, and glycerin.

[0037] In some optional embodiments, the emulsifier includes at least one of rhamnolipid and sophorolipid.

[0038] In some optional embodiments, the emollient includes at least one of squalane, squalene, and vitamin E.

[0039] In some optional embodiments, the pH adjuster includes at least one of caffeic acid, protocatechuic acid, malic acid, citric acid, and lactic acid, or a strong base salt of at least one of them. Given that some ingredients are acidic, strong base salts of such weak acids may also be appropriately used for pH adjustment, such as sodium citrate, disodium succinate, and the like.

[0040] Another embodiment of the present application provides a method for preparing the composition having a hair follicle protecting effect as described in any one of the aforementioned embodiments, comprising:

[0041] Mixing: mixing the hair follicle care agent, moisturizer, emulsifier, emollient and water to obtain a mixed solution;

[0042] pH adjustment, and then adjusting the pH of the mixed solution to 6.0-7.0 using a pH adjuster to obtain the composition.

[0043] In some optional embodiments, the mixing step is carried out at 30°C to 50°C, specifically 30°C, 35°C, 40°C, 45°C, 50°C or any value between 30°C and 50°C. If the temperature of the mixing step is too low or too high, the effect of the composition will be reduced.

[0044] In some optional embodiments, the rotation speed of the mixing step is 500r / min~1000r / min, and the mixing time is 30min~60min. The purpose is to mix the raw materials as fully as possible. Too low a rotation speed and too short a mixing time may cause the raw materials to not be fully mixed. If the mixing time exceeds 60min or the rotation speed exceeds 1000r / min, the mixing effect will not be significantly increased.

[0045] In some optional embodiments, the mixed solution is homogenized for 10 to 20 minutes before adjusting the pH of the mixed solution. The temperature of the homogenization step is 15° C. to 30° C., and the pressure of the homogenization step is 80 MPa to 110 MPa. Homogenization helps improve the stability of the composition. The effect of temperature can be referred to in the mixing step. However, a certain pressure is also required here. High pressure helps improve the mutual solubility of the components.

[0046] In some optional embodiments, the mixing step comprises sequentially adding the hair follicle care agent, emulsifier, moisturizer, and emollient to water at 30°C to 50°C and mixing to obtain a mixed solution. Sequentially adding the hair follicle care agent, emulsifier, moisturizer, and emollient to water facilitates the thorough mixing and combination of the active ingredients.

[0047] Another embodiment of the present application provides an application of the composition with hair follicle protecting effect described in any one of the aforementioned embodiments in daily chemical products.

[0048] In some optional embodiments, the daily chemical product can be used on the face, body or scalp. If used for facial care, it can be prepared into dosage forms such as creams, gels, patches, sprays, ointments, plasters, lotions, liniments, pastes, and mud packs; if used for scalp hair loss prevention, it can be prepared into dosage forms such as conditioners, hair essences, shampoos, hair creams, hair sprays, hair masks, hair soaps, hair cleansing milks, hair oils, hair dyeing aids, and hair sprays.

[0049] The features and performance of the present invention are further described in detail below with reference to the embodiments.

[0050] Example 1

[0051] This embodiment provides a composition having a hair follicle-protecting effect, which is prepared by the following steps:

[0052] (1) Weighing 5 parts by weight of hydroxytyrosol (a hair follicle care agent), 3 parts by weight of sodium hyaluronate (a moisturizer), 10 parts by weight of rhamnolipid (an emulsifier), and 5 parts by weight of squalane (an emollient), respectively, and using citric acid as a pH adjuster for subsequent steps;

[0053] (2) adding 100 parts by weight of deionized water into a reaction kettle, heating to 45° C., and then sequentially adding the hair follicle care agent, emulsifier, moisturizer, and emollient weighed in step (1), and stirring at 1000 rpm for 45 minutes;

[0054] (3) placing the mixed solution stirred in step (2) in a high-pressure homogenizer and homogenizing for 20 minutes;

[0055] (4) Adding a pH regulator to the mixed solution obtained in the above step (3), stirring evenly and adjusting the pH to 6.5, finally obtaining a composition solution with hair follicle protection effect.

[0056] Example 2

[0057] This embodiment provides a composition having a hair follicle-protecting effect, which is prepared by the following steps:

[0058] (1) Weighing 10 parts by weight of oleuropein (a hair follicle care agent), 5 parts by weight of glycerin (a moisturizer), 15 parts by weight of sophorolipid (an emulsifier), and 10 parts by weight of squalene (an emollient), respectively, and using lactic acid as a pH regulator for subsequent steps;

[0059] (2) adding 160 parts by weight of deionized water into a reaction kettle, heating to 30° C., and then sequentially adding the hair follicle care agent, emulsifier, moisturizer, and emollient weighed in step (1) above, and stirring at 800 rpm for 60 min;

[0060] (3) placing the mixed solution stirred in step (2) in a high-pressure homogenizer and homogenizing for 15 minutes;

[0061] (4) Adding a pH regulator to the mixed solution obtained in the above step (3), stirring evenly and adjusting the pH to 6.0, finally obtaining a composition solution with hair follicle protective effect.

[0062] Example 3

[0063] This embodiment provides a composition having a hair follicle-protecting effect, which is prepared by the following steps:

[0064] (1) Weighing 4 parts by weight of hydroxytyrosol as a hair follicle care agent, 1 part by weight of arbutin as a moisturizer, 8 parts by weight of rhamnolipid as an emulsifier, and 3 parts by weight of vitamin E as an emollient, and using caffeic acid as a pH regulator for subsequent steps;

[0065] (2) adding 120 parts by weight of deionized water into a reaction kettle, heating to 35° C., and then sequentially adding the hair follicle care agent, emulsifier, moisturizer, and emollient weighed in step (1) above, and stirring at 500 rpm for 30 minutes;

[0066] (3) placing the mixed solution stirred in step (2) in a high-pressure homogenizer and homogenizing for 10 minutes;

[0067] (4) Adding a pH regulator to the mixed solution obtained in the above step (3), stirring evenly and adjusting the pH to 6.5, finally obtaining a composition solution with hair follicle protection effect.

[0068] Example 4

[0069] This embodiment provides a composition having a hair follicle-protecting effect, which is prepared by the following steps:

[0070] (1) Weighing 6 parts by weight of oleuropein (a hair follicle care agent), 2 parts by weight of sodium hyaluronate (a moisturizer), 7 parts by weight of rhamnolipid (an emulsifier), and 5 parts by weight of squalane (an emollient), respectively, and using protocatechuic acid as a pH regulator for subsequent steps;

[0071] (2) adding 100 parts by weight of deionized water into a reaction kettle, heating to 40° C., and then sequentially adding the hair follicle care agent, emulsifier, moisturizer, and emollient weighed in step (1), and stirring at 700 rpm for 40 minutes;

[0072] (3) placing the mixed solution stirred in step (2) in a high-pressure homogenizer and homogenizing for 15 minutes;

[0073] (4) Adding a pH regulator to the mixed solution obtained in the above step (3), stirring evenly and adjusting the pH to 6.0, finally obtaining a composition solution with hair follicle protective effect.

[0074] Example 5

[0075] This embodiment provides a composition having a hair follicle-protecting effect, which is prepared by the following steps:

[0076] (1) Weighing 8 parts by weight of hydroxytyrosol as a hair follicle care agent, 0.5 parts by weight of arbutin as a moisturizer, 12 parts by weight of sophorolipid as an emulsifier, and 6 parts by weight of squalene as a moisturizer, respectively, and using malic acid as a pH regulator for subsequent steps;

[0077] (2) adding 120 parts by weight of deionized water into a reaction kettle, heating to 50° C., and then sequentially adding the hair follicle care agent, emulsifier, moisturizer, and emollient weighed in step (1), and stirring at 900 rpm for 55 minutes;

[0078] (3) The mixed solution stirred in step (2) was placed in a high-pressure homogenizer and homogenized for 18 minutes;

[0079] (4) Adding a pH regulator to the mixed solution obtained in the above step (3), stirring evenly and adjusting the pH to 6.5, finally obtaining a composition solution with hair follicle protection effect.

[0080] Example 6

[0081] This embodiment provides a composition having a hair follicle-protecting effect, which is prepared by the following steps:

[0082] (1) Weigh 10 parts by weight of hydroxytyrosol (a hair follicle care agent), 4 parts by weight of a moisturizer (2 parts by weight of sodium hyaluronate and 2 parts by weight of glycerin), 15 parts by weight of an emulsifier (9 parts by weight of rhamnolipid and 6 parts by weight of sophorolipid), and 5 parts by weight of an emollient (2 parts by weight of vitamin E and 3 parts by weight of squalane), respectively, and then use a mixture of caffeic acid and protocatechuic acid as a pH regulator for subsequent steps;

[0083] (2) adding 150 parts by weight of deionized water into a reaction kettle, heating to 50° C., and then sequentially adding the hair follicle care agent, emulsifier, moisturizer, and emollient weighed in step (1), and stirring at 1000 rpm for 60 min;

[0084] (3) placing the mixed solution stirred in step (2) in a high-pressure homogenizer and homogenizing for 20 minutes;

[0085] (4) Adding a pH regulator to the mixed solution obtained in the above step (3), stirring evenly and adjusting the pH to 7.0, finally obtaining a composition solution having a hair follicle protecting effect.

[0086] Example 7

[0087] This embodiment provides a composition having a hair follicle-protecting effect, which is prepared by the following steps:

[0088] (1) Weigh 6 parts by weight of oleuropein (a hair follicle care agent), 5 parts by weight of a moisturizer (4 parts by weight of sodium hyaluronate and 1 part by weight of arbutin), 10 parts by weight of an emulsifier (3 parts by weight of rhamnolipid and 7 parts by weight of sophorolipid), and 4 parts by weight of an emollient (2 parts by weight of squalene and 2 parts by weight of squalane), respectively, and use a mixture of malic acid and lactic acid as a pH adjuster for subsequent steps;

[0089] (2) adding 100 parts by weight of deionized water into a reaction kettle, heating to 45° C., and then sequentially adding the hair follicle care agent, emulsifier, moisturizer, and emollient weighed in step (1), and stirring at 600 rpm for 50 min;

[0090] (3) placing the mixed solution stirred in step (2) in a high-pressure homogenizer and homogenizing for 15 minutes;

[0091] (4) Adding a pH regulator to the mixed solution obtained in the above step (3), stirring evenly and adjusting the pH to 6.0, finally obtaining a composition solution with hair follicle protective effect.

[0092] Example 8

[0093] This embodiment provides a composition having a hair follicle-protecting effect, which is prepared by the following steps:

[0094] (1) Weighing 1 part by weight of hydroxytyrosol as a hair follicle care agent, 0.5 part by weight of arbutin as a moisturizer, 2 parts by weight of emulsifiers (1 part by weight of rhamnolipid and 1 part by weight of sophorolipid), and 1 part by weight of vitamin E as an emollient, and then using a mixture of citric acid and lactic acid as a pH regulator for subsequent steps;

[0095] (2) adding 100 parts by weight of deionized water into a reaction kettle, heating to 30° C., and then sequentially adding the hair follicle care agent, emulsifier, moisturizer, and emollient weighed in step (1), and stirring at 500 rpm for 30 minutes;

[0096] (3) placing the mixed solution stirred in step (2) in a high-pressure homogenizer and homogenizing for 10 minutes;

[0097] (4) Adding a pH regulator to the mixed solution obtained in the above step (3), stirring evenly and adjusting the pH to 6.0, finally obtaining a composition solution with hair follicle protective effect.

[0098] Example 9

[0099] This embodiment provides a composition having a hair follicle-protecting effect, which is prepared by the following steps:

[0100] (1) Weigh 3 parts by weight of oleuropein (a hair follicle care agent), 4 parts by weight of a moisturizer (2 parts by weight of glycerin and 2 parts by weight of arbutin), 5 parts by weight of an emulsifier (3 parts by weight of rhamnolipid and 2 parts by weight of sophorolipid), and 3 parts by weight of an emollient (1 part by weight of vitamin E and 2 parts by weight of squalane), respectively, and then use a mixture of caffeic acid and citric acid as a pH adjuster for the subsequent steps;

[0101] (2) adding 200 parts by weight of deionized water into a reaction kettle, heating to 40° C., and then sequentially adding the hair follicle care agent, emulsifier, moisturizer, and emollient weighed in step (1) above, and stirring at 700 rpm for 45 minutes;

[0102] (3) placing the mixed solution stirred in step (2) in a high-pressure homogenizer and homogenizing for 10 minutes;

[0103] (4) Adding a pH regulator to the mixed solution obtained in the above step (3), stirring evenly and adjusting the pH to 6.5, finally obtaining a composition solution with hair follicle protection effect.

[0104] Example 10

[0105] This embodiment provides a composition having a hair follicle-protecting effect, which is prepared by the following steps:

[0106] (1) Weigh 6 parts by weight of hydroxytyrosol (a hair follicle care agent), 5 parts by weight of a moisturizer (3 parts by weight of sodium hyaluronate and 2 parts by weight of arbutin), 10 parts by weight of an emulsifier (5 parts by weight of rhamnolipid and 5 parts by weight of sophorolipid), and 3 parts by weight of an emollient (2 parts by weight of vitamin E and 1 part by weight of squalane), respectively, and use a mixture of malic acid and citric acid as a pH adjuster for subsequent steps;

[0107] (2) adding 200 parts by weight of deionized water into a reaction kettle, heating to 40° C., and then sequentially adding the hair follicle care agent, emulsifier, moisturizer, and emollient weighed in step (1) above, and stirring at 750 rpm for 45 minutes;

[0108] (3) placing the mixed solution stirred in step (2) in a high-pressure homogenizer and homogenizing for 15 minutes;

[0109] (4) Adding a pH regulator to the mixed solution obtained in the above step (3), stirring evenly and adjusting the pH to 7.0, finally obtaining a composition solution having a hair follicle protecting effect.

[0110] Comparative Example 1: Effect of Reaction Temperature on Composition (Comparative Example 1)

[0111] This comparative example provides a composition having a hair follicle protecting effect, which is prepared by the following steps:

[0112] (1) Weighing 5 parts by weight of hydroxytyrosol (a hair follicle care agent), 3 parts by weight of sodium hyaluronate (a moisturizer), 10 parts by weight of rhamnolipid (an emulsifier), and 5 parts by weight of squalane (an emollient), respectively, and using citric acid as a pH adjuster for subsequent steps;

[0113] (2) adding 100 parts by weight of deionized water into a reaction kettle, heating to 80° C., and then sequentially adding the hair follicle care agent, emulsifier, moisturizer, and emollient weighed in step (1), and stirring at 1000 rpm for 45 minutes;

[0114] (3) placing the mixed solution stirred in step (2) in a high-pressure homogenizer and homogenizing for 20 minutes;

[0115] (4) Adding a pH regulator to the mixed solution obtained in the above step (3), stirring evenly and adjusting the pH to 6.5, finally obtaining a composition solution with hair follicle protection effect.

[0116] Comparative Example 2: Effect of pH on Composition (Comparative Example 3)

[0117] This comparative example provides a composition having a hair follicle protecting effect, which is prepared by the following steps:

[0118] (1) Weighing 4 parts by weight of hydroxytyrosol as a hair follicle care agent, 1 part by weight of arbutin as a moisturizer, 8 parts by weight of rhamnolipid as an emulsifier, and 3 parts by weight of vitamin E as an emollient, and using caffeic acid as a pH regulator for subsequent steps;

[0119] (2) adding 120 parts by weight of deionized water into a reaction kettle, heating to 35° C., and then sequentially adding the hair follicle care agent, emulsifier, moisturizer, and emollient weighed in step (1) above, and stirring at 500 rpm for 30 minutes;

[0120] (3) placing the mixed solution stirred in step (2) in a high-pressure homogenizer and homogenizing for 10 minutes;

[0121] (4) Adding a pH regulator to the mixed solution obtained in the above step (3), stirring evenly and adjusting the pH to 2.0, finally obtaining a composition solution having a hair follicle protecting effect.

[0122] Comparative Example 3: Effect of pH on Composition (Comparative Example 5)

[0123] This comparative example provides a composition having a hair follicle protecting effect, which is prepared by the following steps:

[0124] (1) Weighing 8 parts by weight of hydroxytyrosol as a hair follicle care agent, 0.5 parts by weight of arbutin as a moisturizer, 12 parts by weight of sophorolipid as an emulsifier, and 6 parts by weight of squalene as a moisturizer, respectively, and using malic acid as a pH regulator for subsequent steps;

[0125] (2) adding 120 parts by weight of deionized water into a reaction kettle, heating to 50° C., and then sequentially adding the hair follicle care agent, emulsifier, moisturizer, and emollient weighed in step (1), and stirring at 900 rpm for 55 minutes;

[0126] (3) The mixed solution stirred in step (2) was placed in a high-pressure homogenizer and homogenized for 18 minutes;

[0127] (4) Adding a pH regulator to the mixed solution obtained in the above step (3), stirring evenly and adjusting the pH to 11.0, finally obtaining a composition solution having a hair follicle protecting effect.

[0128] Comparative Example 4: Effect of the Content of Some Components on the Composition (Comparative Example 10)

[0129] This comparative example provides a composition having a hair follicle protecting effect, which is prepared by the following steps:

[0130] (1) Weigh 6 parts by weight of hydroxytyrosol (a hair follicle care agent), 10 parts by weight of a moisturizer (3 parts by weight of sodium hyaluronate and 7 parts by weight of arbutin), 10 parts by weight of an emulsifier (5 parts by weight of rhamnolipid and 5 parts by weight of sophorolipid), and 3 parts by weight of an emollient (2 parts by weight of vitamin E and 1 part by weight of squalane), and then use a mixture of malic acid and citric acid as a pH adjuster for subsequent steps;

[0131] (2) adding 200 parts by weight of deionized water into a reaction kettle, heating to 40° C., and then sequentially adding the hair follicle care agent, emulsifier, moisturizer, and emollient weighed in step (1) above, and stirring at 750 rpm for 45 minutes;

[0132] (3) placing the mixed solution stirred in step (2) in a high-pressure homogenizer and homogenizing for 15 minutes;

[0133] (4) Adding a pH regulator to the mixed solution obtained in the above step (3), stirring evenly and adjusting the pH to 7.0, finally obtaining a composition solution having a hair follicle protecting effect.

[0134] Comparative Example 5: Effect of the Content of Some Components on the Composition (Comparative Example 10)

[0135] This comparative example provides a composition having a hair follicle protecting effect, which is prepared by the following steps:

[0136] (1) Weigh 6 parts by weight of hydroxytyrosol (a hair follicle care agent), 5 parts by weight of a moisturizer (3 parts by weight of sodium hyaluronate and 2 parts by weight of arbutin), and 10 parts by weight of an emulsifier (5 parts by weight of rhamnolipid and 5 parts by weight of sophorolipid), respectively, and use a mixture of malic acid and citric acid as a pH adjuster for subsequent steps;

[0137] (2) adding 200 parts by weight of deionized water into a reaction kettle, heating to 40° C., and then sequentially adding the hair follicle care agent, emulsifier, moisturizer, and emollient weighed in step (1) above, and stirring at 750 rpm for 45 minutes;

[0138] (3) placing the mixed solution stirred in step (2) in a high-pressure homogenizer and homogenizing for 15 minutes;

[0139] (4) Adding a pH regulator to the mixed solution obtained in the above step (3), stirring evenly and adjusting the pH to 7.0, finally obtaining a composition solution having a hair follicle protecting effect.

[0140] Comparative Example 6: Effect of the Content of Some Components on the Composition (Comparative Example 10)

[0141] This comparative example provides a composition having a hair follicle protecting effect, which is prepared by the following steps:

[0142] (1) Weighing 6 parts by weight of hydroxytyrosol, a hair follicle care agent, 10 parts by weight of an emulsifier (5 parts by weight of rhamnolipid and 5 parts by weight of sophorolipid), and 3 parts by weight of an emollient (2 parts by weight of vitamin E and 1 part by weight of squalane), respectively, and then using a mixture of malic acid and citric acid as a pH adjuster for subsequent steps;

[0143] (2) adding 200 parts by weight of deionized water into a reaction kettle, heating to 40° C., and then sequentially adding the hair follicle care agent, emulsifier, moisturizer, and emollient weighed in step (1) above, and stirring at 750 rpm for 45 minutes;

[0144] (3) placing the mixed solution stirred in step (2) in a high-pressure homogenizer and homogenizing for 15 minutes;

[0145] (4) Adding a pH regulator to the mixed solution obtained in the above step (3), stirring evenly and adjusting the pH to 7.0, finally obtaining a composition solution having a hair follicle protecting effect.

[0146] Test Example 1: Effect of the composition on the activity of dermal papilla cells under oxidative stress

[0147] Human dermal papilla cells (purchased from Biobw) were inoculated into 96-well cell culture plates, with approximately 5,000 dermal papilla cells per well. The cells were cultured in a cell box for 24 hours to allow the cells to adhere. After the culture was completed, a control group and a test group were set up, and the dermal papilla cells were treated with drugs and cultured for 24 hours:

[0148] 1. The composition of Example 1 + 200 μmol / mL of H2O2;

[0149] 2. Composition of Example 2 + 200 μmol / mL of H2O2;

[0150] 3. Composition of Example 3 + 200 μmol / mL of H2O2;

[0151] 4. Composition of Example 4 + 200 μmol / mL of H2O2;

[0152] 5. Composition of Example 5 + 200 μmol / mL of H2O2;

[0153] 6. Composition of Example 6 + 200 μmol / mL of H2O2;

[0154] 7. Composition of Example 7 + 200 μmol / mL of H2O2;

[0155] 8. Composition of Example 8 + 200 μmol / mL of H2O2;

[0156] 9. Composition of Example 9 + 200 μmol / mL of H2O2;

[0157] 10. The composition of Example 10 + 200 μmol / mL of H2O2;

[0158] 11. 200 μmol / mL H2O2 (control group);

[0159] 12. Comparative Example 1 composition + 200 μmol / mL of H2O2;

[0160] 13. Comparative Example 2 composition + 200 μmol / mL H2O2;

[0161] 14. Comparative Example 3 composition + 200 μmol / mL H2O2;

[0162] 15. Composition of Comparative Example 4 + 200 μmol / mL of H2O2;

[0163] 16. Composition of Comparative Example 4 + 200 μmol / mL of H2O2;

[0164] 17. Composition of Comparative Example 4 + 200 μmol / mL of H2O2.

[0165] Using the CCK-8 cell proliferation activity detection kit, the cell activity detection reagent was prepared at a volume ratio of PBS:CCK-8 of 9:1 under light-proof conditions; after the above-mentioned dermal papilla cell culture was completed, the cell culture medium was discarded and the drug-addition wells were washed with PBS, and then 200 μL of the prepared cell activity detection reagent was added to each well. The cell plate was wrapped with tin foil to avoid light and placed in a cell incubator for incubation for 1 hour.

[0166] After all the above steps are completed, the cell survival rate is measured and calculated. The results are as follows:

[0167]

[0168]

[0169] According to the data of Group 11, when the dermal papilla cells are stimulated by hydrogen peroxide and produce oxidative stress, the survival rate will be greatly reduced.

[0170] According to the data of groups 1 to 10, the combination prepared by this scheme has a good inhibitory effect on the damage caused by oxidative stress, and to a certain extent improves the survival rate of dermal papilla cells under oxidative stress.

[0171] Comparing the data of Groups 1 and 12, it can be seen that when the temperature of the preparation system is too high, some components will decompose and deteriorate. For example, the decomposition and deterioration of hydroxytyrosol and arbutin will produce quinone substances, resulting in the composition not only failing to inhibit the damage caused by oxidative stress, but also causing additional harm to cells, thereby reducing the survival rate.

[0172] Comparison of the data of groups 3 and 12, 5 and 14 shows that in addition to the fact that too high or too low pH itself affects the survival of dermal papilla cells, the resulting deterioration of the composition further reduces the survival rate of dermal papilla cells under oxidative stress.

[0173] Comparing the data of groups 10 and 15, it can be seen that increasing the content of some ingredients cannot effectively improve the efficacy of the composition, which may be related to the fact that they are more susceptible to oxidation and deterioration after the content increases.

[0174] Comparison of the data of groups 10, 16, and 17 shows that when the composition of this embodiment lacks an emollient or a moisturizer, the efficacy of the composition will be reduced to a certain extent, with the lack of an emollient being the most significant.

[0175] Test Example 2: Effect of the composition on reactive oxygen species (ROS) in dermal papilla cells under oxidative stress

[0176] Based on the completion of the dermal papilla cell culture and drug treatment in the above-mentioned Experimental Example 1, the reactive oxygen species (Ros) levels in the dermal papilla cells of each group under oxidative stress were detected: using the DCFH-DA fluorescent probe kit, an appropriate amount of DCFH-DA was added to a rinsed 96-well plate, shaken and placed in an incubator for 30 minutes, and then the 96-well plate was taken out and rinsed.

[0177] The ROS produced in the cells can convert non-fluorescent DCFH into green fluorescent DCF, that is, the green fluorescence intensity indirectly reflects the expression level of ROS. Therefore, after completing the above steps, place the 96-well plate on the microplate reader and use 488nm excitation light and 525nm emission light for excitation. The fluorescence rate of DCF can be measured to indicate the expression level of ROS:

[0178]

[0179]

[0180] According to the data of Group 11, when the dermal papilla cells are stimulated by hydrogen peroxide to produce oxidative stress, the Ros level in the body will increase significantly.

[0181] According to the data of groups 1 to 10, the combination prepared by this scheme has a good inhibitory effect on the damage caused by oxidative stress and can reduce the amount of Ros in dermal papilla cells to a certain extent.

[0182] Comparing the data of groups 1 and 12, it can be seen that the Ros level in the dermal papilla cells is partially reduced compared with that under oxidative stress, which indicates that quinones still have an inhibitory effect on Ros.

[0183] Comparison of the data of groups 3 and 12, and 5 and 14 shows that the deterioration of the composition into quinones also produces an inhibitory effect on Ros.

[0184] Comparison of the data of groups 10 and 15 shows that the increase in the content of some ingredients only slightly reduces the Ros inhibitory effect of the composition.

[0185] Comparison of the data of groups 10, 16, and 17 shows that when the composition of this embodiment lacks an emollient or a moisturizer, the efficacy of the composition will be reduced to a certain extent, with the lack of an emollient being the most significant.

[0186] Combining Experiments 1 and 2, we can see that changing the temperature, pH, and other conditions in the preparation system, or significantly increasing the content of some ingredients, can easily lead to the production of quinones. Furthermore, while quinones can reduce Ros levels in dermal papilla cells under oxidative stress, exerting a certain inhibitory effect on oxidative stress, they can also damage the cells themselves, further reducing cell survival. Therefore, under conditions outside the scope of this solution, the composition will not achieve its protective effect on hair follicles.

[0187] The foregoing description is merely a preferred embodiment of the present invention and is not intended to limit the present invention. Those skilled in the art will readily appreciate that various modifications and variations of the present invention are possible. Any modifications, equivalent substitutions, or improvements made within the spirit and principles of the present invention are intended to be within the scope of protection of the present invention.

Claims

1. A method for preparing a composition having a hair follicle protecting effect, characterized in that: The raw materials include 1 to 10 parts by weight of a hair follicle care agent, 0.5 to 5 parts by weight of a moisturizer, 1 to 15 parts by weight of an emulsifier, 0.5 to 10 parts by weight of an emollient, a pH adjuster, and 0.01 to 1000 parts by weight of water, wherein the hair follicle care agent includes at least one of hydroxytyrosol and oleuropein, and the emollient includes at least one of squalane, squalene, and vitamin E; the pH of the composition is 6.0 to 7.0; the moisturizer includes at least one of sodium hyaluronate, arbutin, and glycerin; the emulsifier includes at least one of rhamnolipid and sophorolipid; and the pH adjuster includes at least one of caffeic acid, protocatechuic acid, malic acid, citric acid, and lactic acid, or a strong base salt of at least one of these. The specific steps include: Mixing the hair follicle care agent, moisturizer, emulsifier, emollient and water to obtain a mixed solution, wherein the mixing step is performed at 30° C. to 50° C.; pH adjustment, and then adjusting the pH of the mixed solution to 6.0-7.0 using a pH adjuster to obtain the composition.

2. The method for preparing the composition having hair follicle protecting effect according to claim 1, characterized in that: The mass fraction of water in the raw material is 50wt%~99wt%.

3. The method for preparing the composition having hair follicle protecting effect according to claim 1, characterized in that: The mass fraction of water in the raw material is 70wt%~99wt%.

4. The method for preparing the composition having hair follicle protecting effect according to claim 1, characterized in that: The raw materials include 3 to 6 parts by weight of a hair follicle care agent, 0.5 to 3 parts by weight of a moisturizer, 1 to 10 parts by weight of an emulsifier, 1 to 5 parts by weight of an emollient, and 100 to 200 parts by weight of water.

5. The method for preparing the composition having hair follicle protecting effect according to claim 1, characterized in that: The rotation speed of the mixing step is 500 r / min to 1000 r / min, and the mixing time is 30 min to 60 min.

6. The method for preparing the composition having hair follicle protecting effect according to claim 1, characterized in that: Before adjusting the pH of the mixed solution, the mixed solution is homogenized for 10 min to 20 min. The temperature of the homogenization step is 15° C. to 30° C., and the pressure of the homogenization step is 80 MPa to 110 MPa.

7. The method for preparing the composition having hair follicle protecting effect according to claim 1, characterized in that: The mixing step is to sequentially add the hair follicle care agent, emulsifier, moisturizer and emollient into water at 30° C. to 50° C. and mix them to obtain a mixed solution.

8. Use of a composition having a hair follicle protecting effect prepared by the preparation method according to any one of claims 1 to 7 in the preparation of daily chemical products.

9. Use of the composition having hair follicle protecting effect according to claim 8 in the preparation of daily chemical products, characterized in that: The dosage form of the daily chemical product is any one of cream, gel, patch, spray, ointment, plaster, emulsion, liniment, paste, pomade, hair conditioner, hair essence, shampoo, hair cream, hair mask, hair soap, hair cleanser, hair oil, hair dyeing auxiliary agent and hair spray.

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