A composition for balancing skin sebum secretion, its preparation method and application

Abstract

This invention provides a composition for balancing skin sebum secretion, its preparation method, and its application. The composition comprises the following components: Lactobacillus fermentation lysate, yeast / zinc fermentation product, niacinamide, zinc PCA, medicinal spore extract, *Salix friesiensis* extract, capryloyl glycine, Ceylon cinnamon bark extract, and sarcosine. The composition for balancing skin sebum secretion provided by this invention utilizes multiple oil-controlling ingredients to inhibit sebum secretion through multiple pathways, while also possessing certain moisturizing and soothing effects.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, specifically relating to a composition for balancing skin sebum secretion, its preparation method, and its application. Background Technology

[0002] Oily skin is characterized by excessive sebum secretion, resulting in a shiny forehead, nose, and face; large pores; blackheads; thick, rough, and dull skin; and a yellowish appearance. In severe cases, it can affect one's appearance. The skin contains numerous sebaceous glands, which are crucial for sebum production. Excessive sebum can lead to other skin conditions such as acne and seborrheic dermatitis. Therefore, effectively regulating sebum secretion and controlling oil production is essential for maintaining healthy, neutral skin. Many factors influence sebum secretion, including endocrine function, skin moisture, age, gender, and diet. However, most of these factors can be controlled through proper cleansing and skincare routines to maintain healthy skin.

[0003] Sebum is produced by sebaceous gland cells in the skin and then secreted onto the skin's surface. The sebum film formed by sebum and sweat on the skin's surface is an important component of the body's first line of defense, and a normal amount of sebum secretion is crucial for maintaining healthy skin. Excessive sebum secretion leads to unpleasant skin appearance and an uncomfortable feel. Excessive sebum on the skin's surface also easily attracts dirt and dust, affecting normal skin function and causing some inconvenience in daily life. Therefore, oil control is an important way to solve these skin problems, and the care of oily skin is also a concern for many consumers seeking improvement.

[0004] Abnormal sebaceous gland secretion is usually caused by excessive androgens. Testosterone and dihydrotestosterone (DHT) are both androgens, and DHT is 5-10 times more active than testosterone. 5α-reductase is the direct catalytic enzyme in the conversion of testosterone to DHT. The expression of DHT affects the proliferation and differentiation of sebaceous gland cells, thus leading to excessive sebum secretion.

[0005] Excessive sebum secretion can lead to increased pressure within the ducts, creating a hypoxic environment. In this environment, Propionibacterium acnes can grow, stimulating keratinocytes to produce pro-inflammatory factors such as IL-1α, TNF-α, and the inflammatory mediator PGE2. When these factors are released during duct rupture, they can trigger a strong local reaction from the innate immune system, resulting in varying degrees of itching, burning, stinging, and even erythema.

[0006] CN108210417A discloses an oil-controlling composition comprising a moisturizer, a free radical scavenger, and an antibacterial agent. The free radical scavenger includes one or more of the following: kiwi fruit extract, dendrobium extract, reed rhizome polysaccharide, and chrysanthemum flavonoids. The addition of a moisturizer forms a water-locking barrier and enhances skin metabolism; the addition of a free radical scavenger reduces oxidative damage and balances sebum secretion; and the addition of an antibacterial agent effectively fights bacteria and inflammation, preventing bacterial infection and maintaining healthy skin tissue. The synergistic effect of these ingredients results in a significant oil-controlling effect.

[0007] CN112089668A discloses a balancing oil-controlling essence and its preparation method, comprising the following raw materials: moisturizer, chelating agent, skin conditioning agent, antioxidant, filler, emulsifier, hair conditioning agent, preservative, fragrance, and water. The moisturizer includes glycerin, propylene glycol, and sodium hyaluronate; the chelating agent includes disodium EDTA; the skin conditioning agent includes ginger root extract, sage leaf extract, horsetail extract, nettle extract, chamomile extract, soapberry extract, and allantoin; the antioxidant includes lemon balm leaf extract, rosemary leaf extract, ginseng root extract, horse chestnut seed extract, and silk fibroin; the filler includes caramel color; the emulsifier includes PEG-40 hydrogenated castor oil; the hair conditioning agent includes coltsfoot leaf extract; the preservative includes dihydroxymethyl imidazolidinyl urea, methylparaben, and iodopropynyl butylcarbamate; and the fragrance includes perfume.

[0008] Research has found that although there are many cosmetics or personal care products on the market that are advertised as having oil-controlling and soothing effects, they generally suffer from poor oil-controlling and soothing effects, complex production processes, and high prices.

[0009] Therefore, developing a composition that can effectively control sebum secretion, balance the epidermal microecology, effectively prevent oily skin, and reduce the content of Propionibacterium acnes is a key research focus in this field. Summary of the Invention

[0010] In view of the shortcomings of the prior art, the purpose of this invention is to provide a composition for balancing skin sebum secretion, its preparation method and application, which can effectively control skin sebum secretion, balance the epidermal microecology, and effectively reduce the content of Propionibacterium acnes on the skin surface, thereby preventing the occurrence of oily skin acne.

[0011] To achieve this objective, the present invention adopts the following technical solution:

[0012] In a first aspect, the present invention provides a composition for balancing sebum secretion in the skin, the composition comprising the following components: lactobacillus fermentation lysate, yeast / zinc fermentation product, nicotinamide, zinc PCA (zinc pyrrolidone carboxylate), medicinal blastocystis extract, fleurone flakes extract, capryloyl glycine, Ceylon cinnamon bark extract, and sarcosine.

[0013] The composition for balancing sebum secretion provided by this invention employs multiple oil-controlling ingredients to form a multi-pathway mechanism to inhibit sebum secretion. Among them, Lactobacillus fermentation lysate can enhance stratum corneum metabolism, remove dead skin, eliminate cellular free radicals, inhibit lipid peroxidation, activate the body's repair capabilities, and inhibit cellular tyrosinase activity, resulting in excellent skin-smoothing and softening effects; Yeast / zinc fermentation product utilizes the zinc enrichment ability of Saccharomyces cerevisiae during its growth and metabolism. Zinc exists in the form of chelates with highly active small molecules such as amino acids and peptides, reducing the skin irritation inherent in inorganic zinc, while simultaneously exerting multiple beneficial skin effects such as anti-inflammatory, acne-reducing, oil-controlling, and dandruff-reducing properties; PCA zinc can control sebum secretion to exert oil-controlling and acne-reducing effects, and can also inhibit Propionibacterium acnes, lipase, and oxidation, thereby reducing irritation, soothing inflammation, and preventing acne formation. The combination of PCA zinc and niacinamide further enhances the efficacy of balancing sebum secretion and preventing acne formation; Medicinal FOMES... The core active ingredients of the OFFICINALIS extract are triterpenes, sesquiterpenes, polyphenols, polysaccharides, and other active components. Its active ingredient content is high, 9.8 times that of general fungal extracts. It not only controls oil and tightens pores but is also suitable for sensitive skin. The EPILOBIUM FLEISCHERI extract, a DSM / Swiss Alpine plant, can balance the epidermal microecology, effectively prevent oily skin, and reduce the content of Propionibacterium acnes. Capryloyl glycine has good antibacterial effects and can also inhibit excessive sebum secretion, exhibiting good skin affinity. The Cinnamon ZEYLANICUM bark extract can effectively inhibit 5α-reductase, control oil and prevent sebum overflow, and has no acne-causing properties. The synergistic effect of capryloyl glycine, Cinnamon ZEYLANICUM bark extract, and sarcosine promotes the combination's efficacy in balancing skin oil secretion and preventing acne formation.

[0014] Preferably, the composition comprises the following components in parts by weight:

[0015] The amount of Lactobacillus fermentation lysate is 20-30 parts, for example, 22 parts, 24 parts, 26 parts, 28 parts, etc.

[0016] The yeast / zinc fermentation product is 0.5-1.5 parts, for example, 0.6 parts, 0.8 parts, 1 part, 1.2 parts, 1.4 parts, etc.

[0017] The amount of nicotinamide is 20-30 parts, for example, 22 parts, 24 parts, 26 parts, 28 parts, etc.

[0018] The zinc content of PCA is 0.2-1 part, for example, it can be 0.4 parts, 0.6 parts, 0.8 parts, etc.

[0019] The medicinal extract of *Polyporus flocculationii* is 0.5-1.5 parts, for example, 0.6 parts, 0.8 parts, 1 part, 1.2 parts, 1.4 parts, etc.

[0020] The extract of *Salix fleurone* is 0.5-1.5 parts, for example, 0.6 parts, 0.8 parts, 1 part, 1.2 parts, 1.4 parts, etc.

[0021] The amount of capryloylglycine is 2-4 parts, for example, 2.5 parts, 3 parts, 3.5 parts, etc.

[0022] The Ceylon cinnamon bark extract is 0.01-0.1 parts, for example, 0.02 parts, 0.04 parts, 0.06 parts, 0.08 parts, etc.

[0023] The amount of creatine is 0.1-0.5 parts, for example, 0.2 parts, 0.3 parts, 0.4 parts, etc.

[0024] Preferably, the composition further includes 30-60 parts of water, for example, 32 parts, 34 parts, 36 parts, 38 parts, 40 parts, 42 parts, 44 parts, 46 parts, 48 ​​parts, 50 parts, 52 parts, 54 parts, 56 parts, 58 parts, etc.

[0025] All other specific point values ​​within the above numerical ranges can be selected, and will not be elaborated on here.

[0026] Preferably, the yeast / zinc fermentation product is obtained by fermentation using Saccharomyces cerevisiae.

[0027] Preferably, the pH value of the yeast / zinc fermentation product is 4-6, for example, it can be 4.5, 5 or 5.5.

[0028] Preferably, the concentration of zinc in the yeast / zinc fermentation product is 150-360 mg / kg, for example, it can be 160 mg / kg, 180 mg / kg, 200 mg / kg, 220 mg / kg, 240 mg / kg, 260 mg / kg, 280 mg / kg, 300 mg / kg, 320 mg / kg, 340 mg / kg, 350 mg / kg, etc.

[0029] Preferably, the mass ratio of the lactobacillus fermentation lysate to the yeast / zinc fermentation product is (20-30):1, for example, it can be 22:1, 24:1, 26:1, 28:1, etc.

[0030] Preferably, the PCA zinc contains 79-82% pyrrolidone carboxylic acid by mass, for example, 80%, 80.5%, 81%, etc.

[0031] Preferably, the zinc content in the PCA zinc is 19.5-21% by mass, for example, it can be 19.8%, 20%, 20.5%, etc.

[0032] Preferably, the mass ratio of nicotinamide to zinc PCA is (40-60):1, for example, it can be 42:1, 45:1, 48:1, 50:1, 52:1, 55:1, etc.

[0033] Preferably, the mass ratio of capryloyl glycine, Ceylon cinnamon bark extract and sarcosine is (35-40):1:(2-3), for example, it can be 36:1:2, 36:1:3, 37:1:2, 37:1:3, 38:1:2, 38:1:3, 39:1:2, 39:1:3, etc.

[0034] All other specific point values ​​within the above numerical ranges can be selected, and will not be elaborated on here.

[0035] In a second aspect, the present invention provides a method for preparing a composition for balancing skin sebum secretion as described in the first aspect, the method comprising the following steps: mixing the lactobacillus fermentation lysate, yeast / zinc fermentation product, nicotinamide, zinc PCA, medicinal blastocystis extract, fleurone extract, capryloyl glycine, Ceylon cinnamon bark extract, sarcosine and water to obtain the composition for balancing skin sebum secretion.

[0036] Thirdly, the present invention provides the application of the composition for balancing skin oil secretion as described in the first aspect in cosmetics and personal care products.

[0037] Compared with the prior art, the present invention has the following beneficial effects:

[0038] The composition for balancing sebum secretion provided by this invention employs multiple oil-controlling ingredients to form a multi-pathway, end-to-end substance that inhibits sebum secretion, while also possessing certain moisturizing and soothing effects. Specifically, the lactobacillus fermentation lysate can enhance stratum corneum metabolism, remove cellular free radicals, and inhibit lipid peroxidation, while the yeast / zinc fermentation product exerts anti-inflammatory, acne-reducing, and oil-controlling effects. The combination of these two ingredients enhances the sebum-balancing effect.

[0039] Zinc PCA can control oil production by regulating sebum secretion and can also inhibit Propionibacterium acnes to prevent acne formation. When used in combination with niacinamide, zinc PCA further enhances its effectiveness in balancing sebum secretion and preventing acne formation.

[0040] Medicinal FOMES OFFICINALIS extract has the effects of controlling oil and tightening pores, and is more suitable for sensitive skin. EPILOBIUM FLEISCHERI extract is a DSM / Swiss Alpine plant that can balance the epidermal microecology and effectively prevent oily skin. When used together, the two can further balance the epidermal microecology.

[0041] Capryloyl glycine has a good antibacterial effect and can also inhibit excessive sebum secretion. Ceylon cinnamon bark extract can effectively inhibit 5α-reductase, control oil and prevent sebum overflow. Capryloyl glycine, Ceylon cinnamon bark extract and sarcosine work synergistically to promote the combination to balance skin oil secretion and prevent acne formation. Attached Figure Description

[0042] Figure 1 The phenotypic diagram of the moisturizing effect of the sample in Example 1 is shown.

[0043] Figure 2 The phenotypic diagram of the soothing efficacy of the sample in Example 1 is shown.

[0044] Figure 3 The image shows the phenotypic effect of the sample in Example 1 on oil control. Detailed Implementation

[0045] The technical solution of the present invention will be further illustrated below through specific embodiments. Those skilled in the art should understand that the embodiments described are merely illustrative of the present invention and should not be construed as limiting the invention in any way.

[0046] The terms “comprising,” “including,” “having,” “containing,” or any other variations thereof, as used herein, are intended to cover non-exclusive inclusion. For example, a composition, step, method, article, or apparatus that includes the listed elements is not limited to those elements and may also include other elements not expressly listed or elements inherent to such composition, step, method, article, or apparatus.

[0047] "Optional" or "any one" means that the matter or event described thereafter may or may not occur, and the description includes both the possibility that the event will occur and the possibility that the event will not occur.

[0048] The indefinite articles “a” and “an” preceding an element or component of this invention do not impose any limitation on the quantity (i.e., number of times) of the element or component. Therefore, “an” or “a” should be interpreted as including one or at least one, and the singular form of an element or component also includes the plural form, unless the quantity clearly refers only to the singular form.

[0049] The terms "one embodiment," "some embodiments," "exemplary," "specific example," or "some examples," etc., used in this invention refer to specific features, structures, materials, or characteristics described in connection with that embodiment or example, which are included in at least one embodiment or example of the invention. In this document, the illustrative expressions of the above terms are not necessarily directed at the same embodiment or example.

[0050] The reagents or instruments used in the following examples are from the following sources:

[0051] Lactobacillus fermentation lysate: Heze High-tech Zone Youke Biotechnology Co., Ltd.;

[0052] Yeast / Zinc Fermentation Products: Active Concepts, LLC (USA);

[0053] Nicotinamide: DSM Nutritional Products Ltd (Swiss DSM Nutritional Products Ltd.);

[0054] PCA Zinc: Konami (Guangzhou) Biotechnology Co., Ltd.;

[0055] Medicinal FOMES OFFICINALIS Extract: Lemei (Guangzhou) Biotechnology Co., Ltd.;

[0056] Epilobium fleischeri extract: DSM Nutritional Products Ltd. (Switzerland DSM Nutritional Products Ltd.);

[0057] Capryloyl glycine: SEPPIC SA (French company);

[0058] Ceylon cinnamon (CINNAMOMUM ZEYLANICUM) bark extract: SEPPIC SA (French company);

[0059] Creatine: SEPPIC SA (French company).

[0060] Example 1

[0061] This embodiment provides a composition for balancing skin oil secretion, the composition comprising the following components in parts by weight:

[0062] 25.17 parts of Lactobacillus fermentation lysate, 0.97 parts of yeast / zinc fermentation product, 25 parts of nicotinamide, 0.5 parts of zinc PCA, 0.96 parts of medicinal spore extract, 0.9 parts of willowherb extract, 3.03 parts of capryloyl glycine, 0.08 parts of Ceylon cinnamon bark extract, 0.21 parts of sarcosine, and 43.18 parts of water.

[0063] Mix all components evenly according to the aforementioned formula to obtain the composition that balances skin oil secretion.

[0064] Example 2

[0065] This embodiment provides a composition for balancing skin oil secretion, the composition comprising the following components in parts by weight:

[0066] 28 parts of Lactobacillus fermentation lysate, 1.4 parts of yeast / zinc fermentation product, 28 parts of nicotinamide, 0.5 parts of zinc PCA, 1.5 parts of medicinal spore extract, 1.5 parts of willowherb extract, 3.1 parts of capryloyl glycine, 0.08 parts of Ceylon cinnamon bark extract, 0.24 parts of sarcosine, and 35.68 parts of water.

[0067] Mix all components evenly according to the aforementioned formula to obtain the composition that balances skin oil secretion.

[0068] Example 3

[0069] This embodiment provides a composition for balancing skin oil secretion, the composition comprising the following components in parts by weight:

[0070] 22 parts of Lactobacillus fermentation lysate, 0.8 parts of yeast / zinc fermentation product, 20 parts of nicotinamide, 0.35 parts of zinc PCA, 0.5 parts of medicinal spore extract, 0.5 parts of willowherb extract, 2 parts of capryloyl glycine, 0.05 parts of Ceylon cinnamon bark extract, 0.1 parts of sarcosine, and 53.7 parts of water.

[0071] Mix all components evenly according to the aforementioned formula to obtain the composition that balances skin oil secretion.

[0072] Example 4

[0073] This embodiment provides a composition for balancing skin sebum secretion. The only difference between this composition and Example 2 is the addition of 28.9 parts of Lactobacillus fermentation lysate and 0.5 parts of yeast / zinc fermentation product. All other raw materials, dosages, and preparation methods are the same as in Example 2.

[0074] Example 5

[0075] This embodiment provides a composition for balancing skin sebum secretion. The only difference between this composition and Example 2 is the addition of 27.9 parts of Lactobacillus fermentation lysate and 1.5 parts of yeast / zinc fermentation product. All other raw materials, dosages, and preparation methods are the same as in Example 2.

[0076] Example 6

[0077] This embodiment provides a composition for balancing skin sebum secretion. The only difference between this composition and that of Example 2 is the addition of 28.1 parts niacinamide and 0.4 parts zinc PCA. All other raw materials, dosages, and preparation methods are the same as in Example 2.

[0078] Example 7

[0079] This embodiment provides a composition for balancing skin sebum secretion. The only difference between this composition and Example 2 is the addition of 27.7 parts niacinamide and 0.8 parts zinc PCA. All other raw materials, dosages, and preparation methods are the same as in Example 2.

[0080] Example 8

[0081] This embodiment provides a composition for balancing skin sebum secretion. The only difference between this composition and that of Example 2 is the addition of 3.26 parts of capryloyl glycine, 0.04 parts of Ceylon cinnamon bark extract, and 0.12 parts of sarcosine. All other raw materials, dosages, and preparation methods are the same as in Example 2.

[0082] Example 9

[0083] This embodiment provides a composition for balancing skin sebum secretion. The only difference between this composition and that of Example 2 is the addition of 3.08 parts of capryloyl glycine, 0.1 parts of Ceylon cinnamon bark extract, and 0.24 parts of sarcosine. All other raw materials, dosages, and preparation methods are the same as in Example 2.

[0084] Example 10

[0085] This embodiment provides a composition for balancing skin sebum secretion. The only difference between this composition and that of Example 2 is the addition of 2.94 parts of capryloyl glycine, 0.08 parts of Ceylon cinnamon bark extract, and 0.4 parts of sarcosine. All other raw materials, dosages, and preparation methods are the same as in Example 2.

[0086] Comparative Example 1

[0087] This comparative example provides a composition for balancing skin sebum secretion. The only difference between this composition and Example 2 is that the amount of Lactobacillus fermentation lysate is adjusted to 29.4 parts, and no yeast / zinc fermentation product is added. All other raw materials, amounts, and preparation methods are the same as in Example 2.

[0088] Comparative Example 2

[0089] This comparative example provides a composition for balancing skin sebum secretion. The only difference between this composition and Example 2 is that the yeast / zinc fermentation product is adjusted to 29.4 parts, and no lactobacillus fermentation lysate is added. All other raw materials, amounts, and preparation methods are the same as in Example 2.

[0090] Comparative Example 3

[0091] This comparative example provides a composition for balancing skin sebum secretion. The only difference between this composition and Example 2 is that the amount of niacinamide is adjusted to 28.5 parts, and zinc PCA is not added. All other raw materials, dosages, and preparation methods are the same as in Example 2.

[0092] Comparative Example 4

[0093] This comparative example provides a composition for balancing skin sebum secretion. The only difference between this composition and Example 2 is that the amount of zinc PCA is adjusted to 28.5 parts, and niacinamide is not added. All other raw materials, dosages, and preparation methods are the same as in Example 2.

[0094] Comparative Example 5

[0095] This comparative example provides a composition for balancing skin sebum secretion. The only difference between this composition and Example 2 is that the amount of capryloyl glycine is adjusted to 3.334 parts and the amount of Ceylon cinnamon bark extract is 0.086 parts. Creatine is not added. All other raw materials, dosages and preparation methods are the same as in Example 2.

[0096] Comparative Example 6

[0097] This comparative example provides a composition for balancing skin sebum secretion. The only difference between this composition and Example 2 is that the amount of capryloyl glycine is adjusted to 3.174 parts and the amount of sarcosine to 0.246 parts, and Ceylon cinnamon bark extract is not added. All other raw materials, dosages and preparation methods are the same as in Example 2.

[0098] Comparative Example 7

[0099] This comparative example provides a composition for balancing skin sebum secretion. The only difference between this composition and Example 2 is that the amount of Ceylon cinnamon bark extract (0.9 parts) and sarcosine (2.52 parts) is adjusted, and capryloyl glycine is not added. All other raw materials, dosages, and preparation methods are the same as in Example 2.

[0100] Comparative Example 8

[0101] This comparative example provides a composition for balancing skin sebum secretion. The only difference between this composition and Example 2 is that the amount of capryloyl glycine is adjusted to 3.42 parts, and Ceylon cinnamon bark extract and sarcosine are not added. All other raw materials, dosages, and preparation methods are the same as in Example 2.

[0102] Comparative Example 9

[0103] This comparative example provides a composition for balancing skin sebum secretion. The only difference between this composition and Example 2 is that the amount of Ceylon cinnamon bark extract was adjusted to 3.42 parts, and capryloyl glycine and sarcosine were not added. All other raw materials, dosages, and preparation methods are the same as in Example 2.

[0104] Comparative Example 10

[0105] This comparative example provides a composition for balancing skin sebum secretion. The only difference between this composition and Example 2 is that the creatine content is adjusted to 3.42 parts, and capryloyl glycine and Ceylon cinnamon bark extract are not added. All other raw materials, dosages, and preparation methods are the same as in Example 2.

[0106] Test Example 1

[0107] Moisturizing effect

[0108] Experimental system: Albino strain zebrafish with melanin allele mutation for translucent color.

[0109] Zebrafish age: 2 days after fertilization (3dpf).

[0110] Sample size per group: 15 tails (N=10).

[0111] Samples: Compositions for balancing skin sebum secretion prepared in Examples 1-10 and Comparative Examples 1-10.

[0112] Adult fish rearing and breeding methods: The rearing and breeding methods shall be carried out in accordance with laboratory standards and meet the requirements of international AAALAC certification (certification number: 001458).

[0113] Test Procedure: 1. Zebrafish were randomly assigned to 6-well plates, 15 fish per well. 2. The sample was administered in water at a concentration of 0.12%. A normal control group and a model control group were also included, with a volume of 3 mL per well. 3. A zebrafish skin dehydration model was established by administering sodium chloride in water. 4. The samples were incubated at 28°C in the dark for 22 hours. 5. Ten zebrafish from each experimental group were randomly selected and photographed under a dissecting microscope. Advanced image processing software was used to analyze and collect data. The zebrafish tail area (S) was analyzed, and the moisturizing effect of the sample was calculated using a formula.

[0114] Moisturizing efficacy (%) = [S(sample group) - S(model control group)] × 100% / [S(normal control group) - S(model control group)]. Statistical analysis: P < 0.05. For example, the phenotypic diagram of the moisturizing efficacy of the sample in Example 1 is shown below. Figure 1 As shown in the figure, the tail area of ​​the zebrafish in the sample group of Example 1 was significantly increased compared with that of the model group, indicating that the sample has a moisturizing effect. The results of the moisturizing effect test are shown in Table 1.

[0115] Test Example 2

[0116] Soothing effect

[0117] Experimental system: transgenic neutrophil-containing green fluorescent zebrafish (MPX).

[0118] Zebrafish age: 2 days after fertilization (2dpf).

[0119] Sample size per group: 15 tails (N=10).

[0120] Samples: Compositions for balancing skin sebum secretion prepared in Examples 1-10 and Comparative Examples 1-10.

[0121] Adult fish rearing and breeding methods: Follow the company's laboratory standards for rearing and breeding methods, which meet the requirements of international AAALAC certification (certification number: 001458).

[0122] Test Procedure: 1. Zebrafish were randomly selected into 6-well plates, 15 fish per well. 2. A zebrafish skin inflammation model was established by administering SLS in water. 3. The sample was administered in water at a concentration of 0.062%. A normal control group and a model control group were also set up, with a volume of 3 mL per well. 4. The samples were incubated at 28℃ in the dark for 18 hours. 5. Ten zebrafish from each experimental group were randomly selected and photographed under a fluorescence microscope. Advanced image processing software was used to analyze and collect data. The number of neutrophils (N) in the zebrafish skin was analyzed, and the soothing effect of the sample was calculated according to the formula to determine whether it had a soothing effect.

[0123] Soothing efficacy (%) = [N(model control group) - N(sample group)] × 100% / [N(model control group) - N(normal control group)]. Statistical analysis: P < 0.05. For example, the phenotypic diagram of the soothing efficacy of the sample in Example 1 is shown below. Figure 2 As shown in the figure, the number of neutrophils in the zebrafish skin of the sample group was significantly reduced compared with that of the model group, indicating that the sample has a soothing effect. The results of the soothing effect test are shown in Table 1.

[0124] Test Example 3

[0125] Oil control effect

[0126] Experimental system: human sebaceous gland cells (SZ95).

[0127] Cell passage number: at least 2 passages.

[0128] Cell count per well: 3 × 10 5 One hole / hole.

[0129] Samples: Compositions for balancing skin sebum secretion prepared in Examples 1-10 and Comparative Examples 1-10.

[0130] Test procedure: 1. Seed cells in 6-well plates (3×10⁶ cells / well). 5 1. Experiments were conducted in three groups (samples / well), including a normal control group, a model control group, and a sample group. The samples were incubated at 37℃ for 24h ± 2h. 2. Drug administration: The normal control group was given fresh culture medium. The model control group was given culture medium containing a certain concentration of testosterone. The sample control group was given diluted sample with a detection concentration of 0.062% and the same testosterone culture medium as the model group. Three parallel experiments were conducted, incubated at 37℃ for 24h ± 2h. 3. Staining: The culture medium was discarded, the samples were washed three times with pre-cooled PBS, fixed with 60% isopropanol, washed with PBS, stained with Nile Red, and photographed under a fluorescence microscope. The fluorescence intensity (A) of each group was analyzed using ImageJ.

[0131] Oil control efficacy (%) = [A(model control group) - A(sample group)] × 100% / A(model control group). Statistical analysis: P < 0.05. For example, the experimental phenotypic diagram of the oil control efficacy of the sample in Example 1 is shown below. Figure 3 As shown in the figure, the fluorescence intensity of the sebaceous gland cells in the sample group was significantly reduced compared to the model group, indicating that the sample has good oil-controlling effects. The results of the oil-controlling effects are shown in Table 1.

[0132] Table 1

[0133]

[0134]

[0135] As shown in the table, all components in this application are essential for the composition that balances sebum secretion. Among them, Lactobacillus fermentation lysate and yeast / zinc fermentation product work synergistically and, when in the proportions adopted in this application, exert excellent moisturizing, soothing, and sebum-balancing effects. Niacinamide and zinc PCA work synergistically and, when in the proportions adopted in this application, further moisturize and balance sebum secretion. Capryloyl glycine, Ceylon cinnamon bark extract, and sarcosine work synergistically and, when in the proportions defined in this application, further soothe the skin and balance sebum secretion. If the proportion of any of these components is too high or too low, it will have an adverse effect.

[0136] Application Example 1

[0137] This application example provides a balancing face cream, which comprises the following components by weight:

[0138] Example 1 prepared a composition for balancing skin sebum secretion consisting of 20 parts, 64.45 parts purified water, 0.2 parts sodium hyaluronate, 3 parts aluminum octenyl succinate starch, 8 parts dipropylene glycol, 1 part cyclopentamethoxysiloxane and cyclohexylsiloxane (purchased from Guangdong Saiante New Materials Co., Ltd.), 1 part polydimethylsiloxane alcohol and polydimethylsiloxane (purchased from Guangdong Saiante New Materials Co., Ltd.), 0.8 parts acrylate / C10-30 alkanol acrylate crosspolymer (purchased from Lubrizol Advanced Materials, Inc.), 1 part polyglycerol-6 distearate and polyglycerol-3 beeswax ester and cetyl alcohol and polyglycerol-3 beeswax ester (purchased from GATTEFOSSE SAS), and 0.55 parts aminomethylpropanol.

[0139] Preparation method:

[0140] 1. Add purified water to the main pot. Without stirring or homogenizing, evenly sprinkle the acrylate / C10-30 alkanol acrylate cross-linked polymer on the water surface. After it absorbs water, swells, and becomes transparent, then jog the homogenizer at 1000 rpm to disperse it evenly. While stirring, add dipropylene glycol and aluminum octenyl succinate starch, and continue to homogenize and stir until evenly mixed. Heat to 85℃ and stir until completely mixed.

[0141] 2. Add cyclopentasiloxane and cyclohexylsiloxane, polydimethylsiloxane alcohol and polydimethylsiloxane, polyglycerol-6 distearate and polyglycerol-3 beeswax ester and cetyl alcohol and polyglycerol-3 beeswax ester to an oil phase pot, heat to 80°C, and stir at 1000 rpm until completely mixed to obtain an oily emollient.

[0142] 3. Pour the dissolved oily emulsifier into the emulsification pot, and stir at 1000 rpm for 8 minutes to disperse and mix it until it is completely uniform and free of particles. Keep it at a constant temperature for 10 minutes.

[0143] 4. Cool down to 42°C, add the composition raw materials for balancing skin oil secretion prepared in Example 1, homogenize and disperse evenly, then add sodium hyaluronate and aminomethylpropanol in sequence, with a 2-minute interval between each addition, and stir evenly.

[0144] 5. When the temperature drops to 40℃, the semi-finished product is sampled and inspected. Once it passes the inspection, it is discharged and the product is obtained.

[0145] Application Example 2-3

[0146] This application example provides a balancing face cream, which differs from Application Example 1 only in that the composition for balancing sebum secretion prepared in Example 1 is replaced with an equal amount of the composition for balancing sebum secretion prepared in Examples 2-3. All other raw materials, dosages, and preparation methods are the same as in Application Example 1.

[0147] Test Example 4

[0148] Human efficacy evaluation test - oil control

[0149] Measuring instrument: Sebumeter (skin oil analyzer).

[0150] The skin sebum secretion test uses the internationally recognized Sebumeter method, which is based on the principle of photometry. A 0.1mm thick matte tape absorbs the sebum on human skin, and the amount of light transmitted changes. The more sebum absorbed, the greater the amount of light transmitted. The content of the initial skin sebum is measured based on the difference in light transmittance.

[0151] Testing environment: Temperature 20.8-21.8℃, relative humidity 51-59%RH.

[0152] The test was conducted according to the T / ZHCA 002-2018 test method for oil control efficacy in cosmetics. Patients with dry, dehydrated skin on both sides of the cheeks and excessive oil secretion on the forehead were selected. Before cleansing, the baseline oil secretion level on the central forehead of women was >100 μg / cm². 3 Before cleaning, the baseline sebum secretion in the central part of a man's forehead is >120 μg / cm². 3 Thirty-five healthy Chinese male and female subjects, aged 18-55 years, were included in the study. Using a product-controlled design, the amount of sebum secreted by the subjects' skin at the test sites was measured before product use, at 2 hours, 4 hours, 8 hours after product use, and after 28 consecutive days of use.

[0153] The specific testing process was as follows: Subjects cleansed their skin with a cleansing product and dried it with a dry facial tissue. They sat quietly in the laboratory for 20 minutes until their skin condition met the testing standards. Then, the right cheek area of ​​each person was divided into four areas, one as a blank control and the other three areas were evenly coated with the product. Technicians measured the skin parameters of the test areas of the subjects at 2 hours, 4 hours, and 8 hours. After that, the subjects used the product twice a day, morning and evening, for 28 consecutive days. After that, the subjects cleaned their skin with the cleansing product, dried it with a dry facial tissue, sat quietly in the laboratory for 20 minutes, and the technicians measured the skin parameters of the test areas of the subjects.

[0154] The amount of sebum secreted by the subjects was measured and the rate of change was calculated. The results are shown in Table 2.

[0155] The test result P < 0.001, where BL - the baseline value of skin parameters before product application to the test site; TX - the value of skin parameters after X hours of product application to the test site, where X is selected from 2, 4, or 8; T28 - the value of skin parameters after 28 days of product application to the test site; the rate of change in oil content (%) after 2, 4, or 8 hours of product application = (TX - BL) × 100% / BL, where X is selected from 2, 4, or 8; the rate of change in oil content (%) after 28 days of product application = (T28 - BL) × 100% / BL.

[0156] Table 2

[0157]

[0158] According to the data in the table, 2 hours, 4 hours, and 8 hours after the subjects used the balancing cream provided by this invention, the amount of sebum secretion in the tested area of ​​the skin increased significantly compared with the baseline value, indicating that the balancing cream provided by this invention has good moisturizing effect, feels good to use, and does not cause tightness. Compared with the blank control, it was found that the balancing cream formulated by this invention has a certain oil control effect while moisturizing. After continuous use of this product for 28 days, the amount of sebum secretion in the subjects' skin decreased significantly compared with the baseline value, indicating that this product plays an important role in balancing skin sebum secretion and effectively regulates the skin condition while moisturizing and soothing.

[0159] The applicant declares that the present invention is illustrated by the above embodiments, but the present invention is not limited to the above process steps, that is, it does not mean that the present invention must rely on the above process steps to be implemented. Those skilled in the art should understand that any improvements to the present invention, equivalent substitutions of the raw materials used in the present invention, addition of auxiliary components, selection of specific methods, etc., all fall within the protection scope and disclosure scope of the present invention.

Claims

1. A composition for balancing sebum secretion in the skin, characterized in that, The composition comprises the following components in parts by weight: 20-30 parts of Lactobacillus fermentation lysate 0.5-1.5 parts of yeast / zinc fermentation product 20-30 parts of nicotinamide PCA zinc 0.2-1 part 0.5-1.5 parts of medicinal Fomitopsis pinnatifida extract 0.5-1.5 parts of *Salix fleurone* extract. 2-4 parts of capryloylglycine Ceylon cinnamon bark extract 0.01-0.1 parts Creatine 0.1-0.5 parts 30-60 parts water; The mass ratio of nicotinamide to zinc PCA is (40-60):1; The mass ratio of capryloyl glycine, Ceylon cinnamon bark extract and sarcosine is (35-40):1:(2-3); The mass ratio of the lactobacillus fermentation lysate to the yeast / zinc fermentation product is (20-30):

1.

2. The composition according to claim 1, characterized in that, The yeast / zinc fermentation product is obtained by fermentation with Saccharomyces cerevisiae.

3. The composition according to claim 1, characterized in that, The pH value of the yeast / zinc fermentation product is 4-6.

4. The composition according to claim 1, characterized in that, The concentration of zinc in the yeast / zinc fermentation product is 150-360 mg / kg.

5. The composition according to claim 1, characterized in that, The PCA zinc contains 79-82% pyrrolidone carboxylic acid by mass.

6. The composition according to claim 1, characterized in that, The zinc content in the PCA zinc is 19.5-21% by mass.

7. A method for preparing a composition for balancing sebum secretion as described in any one of claims 1-6, characterized in that, The preparation method includes the following steps: mixing the Lactobacillus fermentation lysate, yeast / zinc fermentation product, nicotinamide, zinc PCA, medicinal blastocystis extract, Fritillaria ferrofolia extract, capryloyl glycine, Ceylon cinnamon bark extract, sarcosine and water to obtain the composition for balancing skin sebum secretion.

8. The use of a composition for balancing sebum secretion as described in any one of claims 1-6 in the preparation of cosmetics.

9. The use of a composition for balancing sebum secretion as described in any one of claims 1-6 in the preparation of personal care products.

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