A stem cell storage device and method

Abstract

The application discloses a kind of stem cell storage device and method, including refrigerator, the inner cavity formed between the first transverse plate and the second transverse plate of refrigerator;The lower portion of first transverse plate is equipped with motor, the power output shaft of motor passes through first transverse plate and is connected threaded shaft in inner cavity, slidingly arranged with placing plate in inner cavity, threaded hole is opened in placing plate, threaded shaft and threaded hole are mutually engaged and arranged, a plurality of placing grooves are opened in placing plate, the inner cavity of a plurality of placing grooves is equipped with storage tube, the bottom in placing groove is provided with flexible cross opening partition, the edge of cross opening partition is embedded in annular clamping groove opened in placing groove side wall;Second transverse plate is equipped with a plurality of limiting openings, limiting opening is set to correspond a plurality of storage tube top, the outer side edge of a plurality of storage tubes is equipped with clamping device close to bottom end, the lower portion of refrigerator is provided with buffer device.The application effectively stores cold gas to preserve stem cell, and can prevent user from frostbite when accessing.

Description

Technical Field

[0001] This invention relates to the field of stem cell storage technology, and more particularly to a stem cell storage device and method. Background Technology

[0002] Stem cells are a type of pluripotent cell with the ability to self-renew. Under certain conditions, they can differentiate into various functional cells. Based on their developmental stage, stem cells are classified into embryonic stem cells and adult stem cells. Based on their developmental potential, they are classified into three categories: totipotent stem cells, multipotent stem cells, and unipotent stem cells. Stem cells are undifferentiated, immature cells with the potential to regenerate various tissues, organs, and the human body; they are known in the medical field as "universal cells."

[0003] Currently, the storage of stem cells cannot be properly refrigerated throughout the entire chamber, affecting the storage period, causing damage to the stem cells, and impacting their usability. When retrieving stem cells, users need to put their hands into the refrigerated box, which can lead to frostbite. In addition, the cold air inside the container will dissipate, causing the internal temperature to rise, resulting in poor preservation.

[0004] Therefore, a storage device is designed to effectively preserve stem cells and prevent users from being frostbitten during access. Summary of the Invention

[0005] To address the shortcomings of the aforementioned technologies, this invention provides a stem cell storage device and method.

[0006] To solve the above technical problems, the technical solution adopted by the present invention is as follows: a stem cell storage device, including a refrigerator, with an inner cavity formed between a first horizontal plate and a second horizontal plate of the refrigerator; a motor is provided below the first horizontal plate, the power output shaft of the motor passes through the first horizontal plate and is connected to a threaded shaft in the inner cavity, a placement plate is slidably arranged in the inner cavity, a threaded hole is opened on the placement plate, the threaded shaft and the threaded hole are meshed with each other, a plurality of placement slots are opened on the placement plate, and a storage tube is provided in the inner cavity of each of the plurality of placement slots, a flexible cross-shaped opening partition is provided at the bottom of the placement slot, and the edge of the cross-shaped opening partition is embedded in an annular clamping groove opened on the side wall of the placement slot; a plurality of limiting openings are opened on the second horizontal plate, the limiting openings are provided corresponding to the top ends of a plurality of storage tubes, a clamping device is sleeved on the outer edge of each of the plurality of storage tubes near the bottom end, and a buffer device is provided at the bottom of the refrigerator.

[0007] Furthermore, a sealing cover is hinged to the right side of the refrigerator near the top. The bottom of the sealing cover is fitted to the top of the refrigerator. A sealing layer is fixedly connected to the bottom of the sealing cover near the center. The outer edge of the sealing layer is fitted to the inner cavity of the refrigerator.

[0008] Furthermore, the cross-shaped opening partition is composed of four flexible partitions that are inclined downwards from the outside in, and the sides of the four flexible partitions meet on the vertical plane to form a cross-shaped opening.

[0009] Furthermore, the clamping device includes fixed tubes, the bottom ends of which are fixedly connected to the top of the placement plate. Grooves are provided on both the left and right sides of the inner cavity of each fixed tube. Clamping springs are fixedly connected to the side of the adjacent grooves that are far apart from each other, near the top and bottom. Clamping blocks are fixedly connected to the end of the adjacent clamping springs that are close to the adjacent storage tube. The side of the adjacent clamping blocks that are close together is arc-shaped, and the side of the adjacent clamping blocks that are close together is fitted with the adjacent storage tube.

[0010] Furthermore, the buffer device includes dampers fixedly connected to the bottom of the storage box near the four corners, several dampers being fixedly connected to a base at their bottom ends, and locking casters fixedly connected to the bottom of the base near the four corners.

[0011] Furthermore, a refrigerator is fixedly connected to the bottom of the refrigerator cavity near the center, and a cooling pipe is fixedly connected to the top of the first horizontal plate near the center. The bottom end of the cooling pipe and the top of the refrigerator are both connected to a refrigeration pipe. The outer edge of the refrigeration pipe passes through the first horizontal plate near the center. A circular opening is opened on the placement plate near the center, and the top end of the cooling pipe passes through the inner cavity of the circular opening.

[0012] Furthermore, a buffer tube is fixedly connected to the top of the seat near the center, a buffer spring is fixedly connected to the bottom of the inner cavity of the buffer tube, a buffer block is fixedly connected to the top of several buffer springs, a buffer rod is fixedly connected to the top of several buffer blocks, the top of several buffer rods penetrates the inner cavity of the buffer tube and is fixedly connected to a buffer plate, and the top of the buffer plate is fixedly connected to the bottom of the refrigerator near the center.

[0013] Furthermore, rectangular slots are provided on the top of the base near the left and right sides. A linkage block is movably connected to the inner cavity of each of the two rectangular slots. A linkage rod is hinged to the top of each of the two linkage blocks and the bottom of the buffer plate. A through hole is provided near the center of each of the two linkage blocks. A fixing rod is passed through the inner cavity of each of the two through holes. The left and right ends of each of the two fixing rods are fixedly connected to the inner cavity of the adjacent rectangular slot. A linkage spring is fixedly connected to the outer edge of each of the two linkage blocks on the side that is furthest away from each other and the inner cavity of the adjacent rectangular slot. The two linkage springs are sleeved on the outer edge of the adjacent fixing rod.

[0014] A method for preventing cold air loss in a stem cell storage device is characterized in that: when the placement plate moves upward, the cold air in the cavity above the placement plate is compressed, and a pressure difference is generated inside and outside the cross-shaped opening partition in the placement slot, so that the cross-shaped opening is opened by lateral pressure to allow cold air to flow unidirectionally into the cavity below the placement plate.

[0015] A method for preventing frostbite in a stem cell storage device is characterized in that: the frostbite prevention method involves two motor power output shafts driving a threaded shaft to rotate, which in turn drives a placement plate to move up and down inside a refrigerator. The placement plate then drives several storage tubes to move up and down, and the tops of the storage tubes can be moved to the outside of the refrigerator during loading and unloading.

[0016] This invention discloses a stem cell storage device and method. Through the cooperation of various components, the refrigerator can be buffered during movement, thereby protecting the storage tube and preventing damage to the storage tube containing stem cells. The device facilitates the storage and retrieval of stem cells without requiring the user to reach into the refrigerator, effectively preventing frostbite during retrieval. It also ensures that cold air is evenly distributed in the storage tube, effectively preventing cold air loss and avoiding damage to stem cells due to excessive temperature, thus improving the quality of stem cell storage. Attached Figure Description

[0017] Figure 1 This is the front view of the present invention;

[0018] Figure 2 for Figure 1 Enlarged view of point A in the middle;

[0019] Figure 3 for Figure 1 Enlarged view at point B in the middle;

[0020] Figure 4 for Figure 2 Enlarged view at point C;

[0021] Figure 5 This is a top view of the component placement plate of the present invention;

[0022] Figure 6 for Figure 1 Enlarged view of point D in the middle.

[0023] In the diagram: 1. Refrigerator; 2. Motor; 3. Threaded shaft; 4. Placement plate; 5. Slider; 6. Storage tube; 7. Sealing cover; 8. Refrigerator; 9. Cooling pipe; 10. Damper; 11. Base; 12. Fixing tube; 13. Clamping spring; 14. Clamping block; 15. Buffer tube; 16. Buffer spring; 17. Buffer block; 18. Buffer rod; 19. Buffer plate; 20. Linking block; 21. Linking rod; 22. Fixing rod; 23. Linking spring; 24. Movable block; 25. Sealing layer; 26. Cross-shaped opening partition; 27. Annular clamping groove. Detailed Implementation

[0024] It should be noted that, unless otherwise specified, the embodiments and features described in the embodiments of the present invention can be combined with each other. The technical solutions of the present invention will be further described below with reference to the accompanying drawings of the embodiments of the present invention. The present invention is not limited to the following specific embodiments.

[0025] It should be understood that the same or similar reference numerals in the accompanying drawings of the embodiments correspond to the same or similar components. In the description of this invention, it should be understood that if terms such as "upper," "lower," "front," "rear," "left," "right," "top," and "bottom" indicate orientation or positional relationships based on the orientation or positional relationships shown in the accompanying drawings, they are only for the convenience of describing the invention and simplifying the description, and do not indicate or imply that the device or component referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, the terms describing positional relationships in the accompanying drawings are for illustrative purposes only and should not be construed as limiting this patent. Those skilled in the art can understand the specific meaning of the above terms according to the specific circumstances.

[0026] In one embodiment, such as Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 5 , Figure 6As shown, a stem cell storage device includes a refrigerator 1. A first horizontal plate is fixedly connected to the left and right sides near the bottom of the refrigerator 1's inner cavity. A second horizontal plate is fixedly connected to the left and right sides near the top of the refrigerator 1's inner cavity. Bearings are fixedly connected to the left and right sides of the first horizontal plate and the bottom of the second horizontal plate near the left and right sides. A threaded shaft 3 passes through the inner cavity of adjacent bearings. Motors 2 are fixedly connected to the bottom of the refrigerator 1 near the left and right sides. The power output shafts of the two motors 2 are fixedly connected to the bottom ends of adjacent threaded shafts 3. A placement plate 4 is located at the top of the first horizontal plate. Threaded holes are opened on the placement plate 4 near the left and right sides. The outer edges of the two threaded shafts 3 pass through the inner cavities of adjacent threaded holes, and the outer edges of the two threaded shafts 3 are mutually engaged with the inner cavities of adjacent threaded holes. Slider 5s are fixedly connected to the left and right sides of the placement plate 4. The refrigerator 1 has sliding grooves on both sides near the top of the inner cavity. Two sliders 5 are located in the inner cavity of adjacent sliding grooves. The top of the placement plate 4 has several placement slots near the edge. Each placement slot has a storage tube 6. The bottom of the placement slot is provided with a flexible cross-shaped partition 26. The cross-shaped partition 26 matches the shape of the lower end of the storage tube 6 to stably place the storage tube 6. The edge of the cross-shaped partition 26 is embedded in the annular clamping groove 27 on the side wall of the placement slot. The cross-shaped partition 26 is composed of four flexible partitions that are inclined downward from the outside to the inside. The flexible partitions are made of rubber or flexible plastic. The sides of the four flexible partitions meet on the vertical plane to form a cross-shaped opening. When the gas pressure in the placement slot is greater than the pressure difference on the other side of the cross-shaped partition 26, the cross-shaped opening is opened by lateral pressure. Several limiting openings are provided near the edge of the second horizontal plate. The tops of several storage tubes 6 penetrate the inner cavities of adjacent limiting openings. Clamping devices are provided near the bottom of the outer edges of several storage tubes 6. The clamping devices include fixing tubes 12 located near the bottom of the outer edges of several storage tubes 6. The bottom ends of several fixing tubes 12 are fixedly connected to the top of the placement plate 4. Grooves are provided on both the left and right sides of the inner cavities of several fixing tubes 12. Clamping springs 13 are fixedly connected to the top and bottom of the opposite sides of the inner cavities of adjacent grooves. Clamping blocks 14 are fixedly connected to the ends of adjacent clamping springs 13 near adjacent storage tubes 6. The adjacent sides of adjacent clamping blocks 14 are arc-shaped and are in close contact with adjacent storage tubes 6. Movable blocks 24 are fixedly connected to the top and bottom near the center of several clamping blocks 14. Limiting grooves are provided at the top and bottom of several grooves. Movable blocks 24 are located in the inner cavities of adjacent limiting grooves.

[0027] In this embodiment, as Figure 1As shown, a sealing cover 7 is hinged to the right side of the refrigerator 1 near the top. The bottom of the sealing cover 7 is fitted to the top of the refrigerator 1. A sealing layer 25 is fixedly connected to the bottom of the sealing cover 7 near the center. The outer edge of the sealing layer 25 is fitted to the inner cavity of the refrigerator 1.

[0028] To facilitate the movement and fixation of the device, in this embodiment, such as Figure 1 As shown, a buffer device is provided at the bottom of the refrigerator box 1. The buffer device includes dampers 10 connected near the four corners of the bottom of the refrigerator box 1. The bottom ends of several dampers 10 are fixedly connected to a base 11. Locking casters are fixedly connected to the bottom of the base 11 near the four corners.

[0029] During movement, the refrigerator 1 is cushioned, thereby protecting the storage tube 6 and preventing damage to the tube containing stem cells. In this embodiment, for example... Figure 3 As shown, a buffer tube 15 is fixedly connected to the top of the base 11 near the center. A buffer spring 16 is fixedly connected to the bottom of the inner cavity of the buffer tube 15. Buffer blocks 17 are fixedly connected to the tops of several buffer springs 16. Buffer rods 18 are fixedly connected to the tops of several buffer blocks 17. The tops of several buffer rods 18 penetrate the inner cavity of the buffer tube 15 and are fixedly connected to a buffer plate 19. The top of the buffer plate 19 is fixedly connected to the bottom of the refrigerator 1 near the center. Rectangular grooves are opened on the top of the base 11 near the left and right sides. The inner cavity of each groove is movably connected with a linkage block 20. The top of each linkage block 20 is hinged to the bottom of the buffer plate 19 with a linkage rod 21. Each linkage block 20 has a through hole near the center. The inner cavity of each through hole is provided with a fixing rod 22. The left and right ends of each fixing rod 22 are fixedly connected to the inner cavity of the adjacent rectangular groove. The side of each linkage block 20 that is far apart from the other side is fixedly connected to the inner cavity of the adjacent rectangular groove with a linkage spring 23. Each linkage spring 23 is sleeved on the outer edge of the adjacent fixing rod 22.

[0030] The stem cells in storage tube 6 can be effectively cryopreserved. In this embodiment, for example... Figure 1 As shown, a refrigerator 8 is fixedly connected to the bottom of the inner cavity of the refrigerator 1 near the center, and a cooling pipe 9 is fixedly connected to the top of the first horizontal plate near the center. The bottom end of the cooling pipe 9 and the top of the refrigerator 8 are connected to a refrigeration pipe. The outer edge of the refrigeration pipe passes through the first horizontal plate near the center. A circular opening is opened on the placement plate 4 near the center, and the top end of the cooling pipe 9 passes through the inner cavity of the circular opening.

[0031] Working Principle: In use, the device is easily moved by several locking casters, improving ease of use. After being moved to the desired position, the locking casters are locked to secure the device, preventing slippage and increasing stability. During movement, the damper 10, buffer tube 15, buffer spring 16, buffer block 17, buffer rod 18, buffer plate 19, linkage block 20, linkage rod 21, fixing rod 22, and linkage spring 23 buffer the refrigerator 1, thus protecting the storage tube 6 and preventing damage to the storage tube. If the stem cell storage tube 6 is damaged, the stem cells in the storage tube 6 can be effectively refrigerated by the refrigerator 8 and the cooling pipe 9. When it is necessary to remove the storage tube 6, the sealing cover 7 is opened, and the two motors 2 are started. The power output shafts of the two motors 2 drive the adjacent threaded shafts 3 to rotate, and the two threaded shafts 3 together drive the placement plate 4 to move upward. The placement plate 4 can drive several storage tubes 6 to move upward, so that the top of the storage tubes 6 moves to the outside of the refrigerator 1, which makes it easier for the staff to remove the storage tubes 6. As the placement plate 4 moves upward, the cold air in the cavity above the placement plate 4 is compressed, so that the cold air in the placement slot is compressed. The internal cold air pressure is greater than the pressure difference on the other side of the cross-shaped partition 26, causing the cross-shaped opening to be opened by lateral pressure. Cold air flows through the cross-shaped opening into the cavity below the placement plate 4 via the cavity above the placement plate 4, achieving instantaneous transfer of cold air during the upward movement of the placement plate 4. This is one of the functions of the cross-shaped partition 26. Because the cross-shaped opening of the cross-shaped partition 26 is composed of four flexible partitions that slope downwards from the outside in, and the cross-shaped openings are joined together on the vertical plane, forming an overall downward convex shape, it requires greater lateral pressure. This is not conducive to the entry of cold air from the cavity below the placement plate 4 into the cavity above. During the instantaneous upward movement of the placement plate 4, the placement plate... As the volume of the upper cavity of the placement plate 4 gradually decreases, the volume of the lower cavity of the placement plate 4 gradually increases. Therefore, it is impossible to meet the condition of forming a larger lateral pressure in the lower cavity of the placement plate 4. This causes the cross-shaped opening partition 26 to momentarily block the cold air entering the lower cavity of the placement plate 4. This is the second function of the cross-shaped opening partition 26. During the momentary upward movement of the placement plate 4, the flow of cold air passes through the placement groove, and the bottom of the storage tube 6 is located in the placement groove. Therefore, enhanced cooling is formed for the bottom of the storage tube 6 before it is removed. This is the third function of the cross-shaped opening partition 26. The cold air flowing outside the storage tube 6 has a enhanced cooling effect on the stem cells inside the storage tube 6.

[0032] When it is necessary to place the storage tube 6 into the inner cavity of the refrigerator 1, the bottom end of the storage tube 6 is inserted into the adjacent through-limit opening and into the adjacent placement slot. By starting the two motors 2 and rotating their power output shafts in opposite directions, several storage tubes 6 can be moved into the inner cavity of the refrigerator 1. This facilitates the placement of the storage tube 6 into the inner cavity of the refrigerator 1. The fixed tube 12, clamping spring 13, and clamping block 14 can clamp and fix the storage tube 6, improving the stability of the storage tube 6 when moving up and down. This makes the device convenient for storing and retrieving stem cells without requiring the user to insert their hands into the refrigerator 1. The cold air is easily dissipated, thus effectively preventing frostbite during user retrieval. Because several storage tubes 6 are arranged in a ring around the cooling tube 9, the cold air can be evenly distributed within the storage tubes 6. As the placement plate 4 moves downward, the cold air is compressed in the cavity below the placement plate 4. Over time, it gradually diffuses into the inner cavity of the refrigerator 1, i.e., the cavity above the placement plate 4, through the gap between the refrigerator 1 and the placement plate 4 and the slider 5. Furthermore, the sealing cover 7, sealing layer 25, and second horizontal plate effectively prevent the loss of cold air from above, avoiding damage to stem cells due to excessive temperature and improving the storage quality of stem cells.

[0033] The technical features of the above embodiments can be combined in any way. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

Claims

1. A stem cell storage device, characterized in that: The refrigerator includes a first horizontal plate and a second horizontal plate forming an inner cavity. A motor is located below the first horizontal plate, and the motor's power output shaft passes through the first horizontal plate and connects to a threaded shaft within the inner cavity. A placement plate is slidably arranged within the inner cavity, and the placement plate has threaded holes. The threaded shaft and the threaded holes mesh with each other. The placement plate has several placement slots, and each placement slot has a storage tube inside. A flexible cross-shaped opening partition is provided at the bottom of the placement slot, and the edge of the cross-shaped opening partition is embedded in an annular clamping groove provided on the side wall of the placement slot. The second horizontal plate has several limiting openings, which are provided at the top of several storage tubes. Clamping devices are fitted on the outer edges of several storage tubes near their bottom ends. A buffer device is provided at the bottom of the refrigerator. The cross-shaped opening partition is composed of four flexible partitions that slope downwards from the outside to the inside. The sides of the four flexible partitions meet in the vertical plane to form a cross-shaped opening.

2. The stem cell storage device according to claim 1, characterized in that: A sealing cover is hinged to the right side of the refrigerator near the top. The bottom of the sealing cover is fitted to the top of the refrigerator. A sealing layer is fixedly connected to the bottom of the sealing cover near the center. The outer edge of the sealing layer is fitted to the inner cavity of the refrigerator.

3. The stem cell storage device according to claim 1, characterized in that: The clamping device includes fixed tubes, the bottom ends of which are fixedly connected to the top of the placement plate. Grooves are provided on both the left and right sides of the inner cavity of each fixed tube. Clamping springs are fixedly connected to the opposite sides of the inner cavities of adjacent grooves near the top and bottom. Clamping blocks are fixedly connected to the adjacent storage tubes at their respective ends. The adjacent clamping blocks are arc-shaped on their adjacent sides, and the adjacent clamping blocks are in close contact with the adjacent storage tubes.

4. The stem cell storage device according to claim 1, characterized in that: The buffer device includes dampers fixedly connected to the bottom of the refrigerator near the four corners, and a base fixedly connected to the bottom of several dampers. Locking casters are fixedly connected to the bottom of the base near the four corners.

5. The stem cell storage device according to claim 2, characterized in that: A refrigerator is fixedly connected to the bottom of the inner cavity of the refrigerator near the center. A cooling pipe is fixedly connected to the top of the first horizontal plate near the center. The bottom end of the cooling pipe and the top of the refrigerator are both connected to a refrigeration pipe. The outer edge of the refrigeration pipe passes through the first horizontal plate near the center. A circular opening is opened near the center of the placement plate. The top end of the cooling pipe passes through the inner cavity of the circular opening.

6. The stem cell storage device according to claim 4, characterized in that: A buffer tube is fixedly connected to the top of the base near the center. A buffer spring is fixedly connected to the bottom of the inner cavity of the buffer tube. A buffer block is fixedly connected to the top of several buffer springs. A buffer rod is fixedly connected to the top of several buffer blocks. The top of several buffer rods penetrates the inner cavity of the buffer tube and is fixedly connected to a buffer plate. The top of the buffer plate is fixedly connected to the bottom of the refrigerator near the center.

7. The stem cell storage device according to claim 6, characterized in that: The base has rectangular slots on its top near the left and right sides. Each of the two rectangular slots has a connecting block movably connected to its inner cavity. The top of each of the two connecting blocks is hinged to the bottom of the buffer plate and has a connecting rod. Each of the two connecting blocks has a through hole near its center. Each of the two through holes has a fixing rod passing through its inner cavity. The left and right ends of each of the two fixing rods are fixedly connected to the inner cavities of the adjacent rectangular slots. The two connecting blocks have a connecting spring fixedly connected to the inner cavities of the adjacent rectangular slots on their opposite sides. Each of the two connecting springs is sleeved on the outer edge of the adjacent fixing rod.

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