A mite-killing composition, a mite-killing additive containing the composition, and a preparation method and application thereof

Abstract

The application discloses a kind of acaricidal compositions, acaricidal additive containing the composition and its preparation method and application.The composition includes 4-terpineol, menthol, borneol, artificial ox gallstone, Ma Yinglong raw powder, mountain chicken pepper fruit oil, clove bud extract, thymus vulgaris extract, neem seed extract.The acaricidal additive includes the above-mentioned composition, and also contains polyhydric alcohol, emulsifier, preservative, deionized water.The application also provides a kind of application of acaricidal additive in the preparation of cream, emulsion, water agent or patch with acaricidal efficacy.The composition of the application not only improves the acaricidal effect of product, reduces the irritability of product, but also has good antipruritic effect.

Description

Technical Field

[0001] This invention relates to the field of daily chemical products technology, specifically to a mite-removing composition, a mite-removing additive containing the composition, its preparation method, and its application. Background Technology

[0002] Demodex mites are small, permanent parasitic mites that infest the hair follicles and sebaceous glands of humans and mammals. They are mainly divided into two types: Demodex folliculorum and Demodex brevis. Foreign scholars have reported an infection rate of 27.00%–100.00% in the human population, and infection is also widespread in China, with an infection rate ranging from 20.00% to 97.86%. Demodex mites parasitize the hair follicles and sebaceous glands of the human face, neck, chest, shoulders, back, nipples, vulva, and anus, with the highest infection rate on the face (including cheeks, eyelids, and external auditory canal).

[0003] Demodex mites cause diffuse redness, congestion, scattered pinhead- to millet-sized red papules, nodules, pustules, crusts, abnormal sebum secretion, significant dilation of hair follicles, and rough or even uneven skin surface by piercing and sucking the contents of epithelial cells and feeding on sebum, keratin, and cellular metabolites. This leads to diseases such as folliculitis, seborrheic dermatitis, seborrheic alopecia, acne, rosacea, blepharitis, and external auditory canal pruritus. Among these, the incidence of ocular Demodex mite infection is increasing. Patients with ocular Demodex mite infection may experience ocular surface lesions such as abnormal eyelashes, recurrent styes, conjunctivitis, meibomian gland dysfunction, and decreased tear film stability. Some patients with Demodex mite infection may even develop severe lesions such as limbal stem cell deficiency and corneal neovascularization, posing a significant threat to human health. In addition, Demodex mite infection often causes itching, especially in ocular and external auditory canal infections, where symptoms such as eye itching and external auditory canal itching are more severe.

[0004] To address the aforementioned issues, various mite-removing products are currently available on the market, but these products suffer from inconsistent quality, poor efficacy, and high irritation. For example, patent application number 201910044196.7 discloses a method for preparing a natural antibacterial and mite-removing agent. This natural antibacterial and mite-removing agent is prepared from extracts of Litsea cubeba, Sophora flavescens, clove flowers, or thyme. The resulting product has poor mite-removing efficacy and no antipruritic effect. Summary of the Invention

[0005] The purpose of this invention is to solve the above-mentioned technical problems and provide a mite-removing composition that not only improves the mite-removing effect of the product and reduces the product's irritation, but also has a good antipruritic effect.

[0006] A second objective of the present invention is to provide a mite-removing additive containing the above-described composition.

[0007] A third objective of this invention is to provide a method for preparing the above-mentioned additive.

[0008] A fourth objective of this invention is to provide an application of the above-mentioned additive in creams, lotions, liquids or patches with mite-removing effects.

[0009] To achieve the above objectives, the present invention adopts the following technical solution.

[0010] A mite-removing composition, by weight, comprises the following raw materials:

[0011]

[0012] The raw powder of Mayinglong is a mixture of zinc carbonate hydroxide, amber powder, and pearl powder.

[0013] Preferably, the mite-removing composition comprises the following raw materials in parts by weight:

[0014]

[0015] The ratio of zinc carbonate hydroxide, amber powder, and pearl powder in the Mayinglong raw powder is (80-100):(0.1-2):(0.1-2). Preferably, the ratio of zinc carbonate hydroxide, amber powder, and pearl powder in the Mayinglong raw powder is (90-100):(0.1-1):(0.5-2).

[0016] Litsea cubeba fruit oil, also known as mountain pepper oil or litsea cubeba oil, has the effects of mite removal, sterilization, anti-inflammation, and astringency in the composition of this application. Litsea cubeba fruit oil also has the aroma of lemon, and its main component, citral, also plays a role in enhancing the aroma and masking the smell of traditional Chinese medicine.

[0017] Clove (EUGENIA CARYOPHYLLUS) flower bud extract has an inhibitory effect on 5α-reductase. The eugenol, β-caryophyllene and other components contained in clove flowers have significant functions such as antibacterial and anti-inflammatory effects, free radical scavenging, and analgesia. Eugenin has good antibacterial activity against both bacteria and fungi, and the product has the characteristics of high efficiency and low toxicity.

[0018] Thyme (Thymus sempyllum) extract has significant antibacterial and insecticidal activity with a broad spectrum. It inhibits most Gram bacteria, suppresses fungal cell growth, and has anti-inflammatory effects. It also has antibacterial and bacteriostatic effects on various body odor-causing bacteria and can be used as an odor suppressant, antibacterial agent, and anti-inflammatory agent. In addition, thyme extract can also work synergistically with other substances to enhance antibacterial and insecticidal activity.

[0019] Melia azadiracta seed extract is a traditional Indian medicine containing limonene analogues (azacitin, neemtriol, azadirachtin, etc.). It can kill mites, bacteria, fungi, and viruses, and also has pharmacological activities such as antipyretic analgesia, immune regulation, anti-ulcer, and wound healing promotion. It has a good synergistic effect on mite control.

[0020] 4-Trinol is the main component and key active ingredient of tea tree oil. The quality of tea tree oil is mainly judged by its 4-terpineol content, which is mostly obtained through distillation and purification from tea tree oil. At room temperature, it is a yellow, transparent liquid that is volatile and has a unique, pleasant aroma. It possesses antioxidant, antibacterial, insecticidal, and acaricidal properties, and is mainly used as an anti-inflammatory agent, antiparasitic agent, and fragrance agent. It can be widely used to treat acne, rosacea, and Demodex mite infestation.

[0021] Menthol, also known as peppermint, is a terpenoid organic compound extracted from the leaves and stems of mint. It is commonly added to topical preparations for skin use, providing local relief of itching, pain, cooling sensations, and mild local anesthesia. Furthermore, due to the presence of the skin's stratum corneum barrier and the inherent physicochemical properties of drugs, many medications have very low permeability through the skin, making it difficult to achieve effective therapeutic concentrations. Menthol, however, significantly promotes the transdermal penetration of various active ingredients.

[0022] Zinc carbonate hydroxide, also known as calamine (calcined), is a mineral powder with detoxifying, vision-improving, and anti-pterygium properties. It is used for red, swollen, and painful eyes, inflamed eyelids, pterygium, non-healing ulcers, oozing pus, eczema, and itchy skin. Modern pharmacological studies have shown that topical application of zinc carbonate hydroxide can inhibit the growth of local Staphylococcus aureus and also has a hygroscopic effect, reducing external irritation to the skin, thereby achieving antipruritic, anti-inflammatory, and skin-soothing effects.

[0023] Pearl powder has the effects of clearing heat and moisturizing, improving eyesight and removing corneal opacity, detoxifying and promoting tissue regeneration. Interstitial fluid can form a hypertonic state, which can reduce swelling and inflammation. Externally, it can be used to improve red eyes with corneal opacity, sores that do not heal, and skin pigmentation.

[0024] Amber powder is used to treat infantile convulsions, epilepsy, palpitations, insomnia, hematuria, urinary retention, amenorrhea, postpartum abdominal pain due to blood stasis, carbuncles, boils, and traumatic injuries. Amber can also penetrate the skin to promote blood circulation.

[0025] Artificial bezoar has the effects of detoxifying, resolving phlegm, and calming the nerves. It is used for febrile coma, stroke with phlegm obstruction, convulsions, epilepsy, sore throat, oral ulcers, carbuncles, boils, and sores. Its active ingredients, such as taurine deoxycholic acid, can enhance the phagocytic function of peripheral blood phagocytes and inhibit delayed-type hypersensitivity reactions.

[0026] Borneol has the effects of clearing the mind and relieving pain, and is used for symptoms such as delirium due to obstruction of consciousness, red and swollen eyes, sore throat and mouth ulcers, and carbuncles that fail to heal after ulceration. Modern pharmacological studies have shown that local application of borneol has a slight stimulating effect on sensory nerves, and has certain analgesic and mild antiseptic effects. Higher concentrations (0.5%) have inhibitory effects on Staphylococcus, Streptococcus, Streptococcus pneumoniae, Escherichia coli, and some pathogenic dermatophytes.

[0027] It is understood that the above composition can also be appropriately supplemented with excipients other than the above-mentioned active ingredients to make product additives. The excipients also include polyols, emulsifiers, thickeners, preservatives, deionized water, etc.

[0028] An anti-mite additive containing the above-mentioned composition further includes 1-80 parts of polyol, 0.1-20 parts of emulsifier, 0.1-2 parts of preservative, and 10-95 parts of deionized water.

[0029] The polyol is selected from at least one of glycerol, 1,2-propanediol, 1,3-propanediol, butylene glycol, sorbitol, dipropylene glycol, pentanediol, decanediol, octanediol, and octanediol.

[0030] The emulsifier is selected from at least one of polysorbate-20, polysorbate-80, PEG-40 hydrogenated castor oil / decyl alcohol polyether-7 / PPG-26-butanol polyether-26, polyglycerol-6 caprylate / polyglycerol-3 cocoate / polyglycerol-4 decanoate / polyglycerol-6 ricinoleate.

[0031] The preservative is selected from at least one of phenoxyethanol, ethylhexylglycerin, chlorphenesin, methylparaben, p-hydroxyacetophenone, and 1,2-hexanediol.

[0032] A method for preparing the aforementioned mite-removing additive includes the following steps:

[0033] (1) Pulverize borneol, menthol and artificial bezoar into fine powder, add them together with Mayinglong raw powder into emulsifier, mix and stir and heat, and after complete dissolution, cool down to obtain the first mixture;

[0034] (2) Add 4-terpineol, Litsea cubeba fruit oil, Eugenia caryopsis flower bud extract, Thymus sempylum extract, and Melia azadiracta seed extract to the first mixture in step (1) and mix well to obtain the second mixture.

[0035] (3) Add the polyol and preservative to the second mixture obtained in step (2) and stir until homogeneous to obtain the third mixture;

[0036] (4) Add deionized water to the third mixture obtained in step (3) and stir evenly to obtain the mite removal additive.

[0037] In step (1), the temperature is raised to 70-80°C by stirring.

[0038] The application of a mite-removing additive, wherein the additive is used in the preparation of cosmetics, disinfection products or daily necessities with mite-removing effects.

[0039] The cosmetics include creams, lotions, liquids, patches, and films, with specific products including shampoos, shower gels, face creams, lotions, toners, liquid sprays, face masks, and eye masks; the disinfection products include disinfectants and hygiene products, with specific products including disinfectant solutions, wet wipes, and sanitary wipes; the daily necessities include cotton pads.

[0040] Preferably, the additive is used in the preparation of eye mite removal products. These eye mite removal products include eye mite removal pads, eye mite removal wipes, mite removal eye patches, and eye mite removal sprays.

[0041] It should be noted that the additive described in this invention is a liquid formulation that can be directly added to products of various dosage forms. The application method is simple, and the amount of product added can be selected according to actual needs.

[0042] Compared with the prior art, the present invention has the following beneficial effects:

[0043] 1. Based on the mite-removing effect, this invention selects plant extracts with mite-removing and antibacterial effects, such as Litsea cubeba fruit oil, clove bud extract, thyme extract, and neem seed extract, which greatly improve the mite-removing efficacy of the product. Through experiments, this invention unexpectedly discovered that the neem seed extract has a very significant synergistic effect on the mite-removing efficacy of the product.

[0044] 2. The present invention further selects 4-terpineol and menthol, which have the effects of eliminating mites and relieving itching, to further improve the mite-eliminating and antipruritic effects of the product, and menthol has a significant promoting effect on the transdermal penetration of various functional ingredients.

[0045] 3. The composition of the present invention has good mite-removing and antipruritic effects and low irritation, and is especially suitable for use in eye products. When made into mite-removing cotton pads, it can be used to clean the eyelid margin and can effectively improve the symptoms of eye diseases such as Demodex blepharitis and meibomian gland dysfunction.

[0046] 4. The active ingredients in this invention, namely zinc carbonate hydroxide, pearl powder, amber powder, borneol, and artificial bezoar, are derived from the ancient formula of Ma Yinglong's Eight Treasures. Together, they have the effects of clearing heat and reducing redness, detoxifying and improving eyesight, sterilizing and relieving itching, and reducing irritation. In particular, zinc carbonate hydroxide can react with free fatty acids in tears to produce zinc oleate and zinc fatty acid, which not only moisturize and protect the eyes, but also have the functions of absorption and relieving itching. It can also be absorbed into cells to promote the synthesis of nucleic acids and nucleoproteins, participate in the energy metabolism of cells, and play a role in promoting tissue repair.

[0047] 5. The present invention also provides an additive with mite-removing effect and its preparation method. The additive has wide application, simple preparation method, strong universality, and is suitable for large-scale production.

[0048] In summary, this invention, through the careful selection and scientific formulation of various effective ingredients for eliminating mites, inhibiting bacteria, and relieving itching, produces a product with outstanding mite-eliminating and anti-itch effects and low irritation, solving the problem of poor efficacy and high irritation of conventional mite-eliminating products on the market. The various effective ingredients in this invention are not simply superimposed, but rather scientifically and rigorously formulated in terms of type and dosage, with each ingredient working synergistically and complementing the others. Attached Figure Description

[0049] Figure 1a The chicken embryo chorioallantoic membrane before sample processing;

[0050] Figure 1b The chicken embryo chorioallantoic membrane after sample treatment. Detailed Implementation

[0051] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0052] It should be noted that the phenoxyethanol / ethylhexylglycerin used in the examples is commercially known as euxyl PE9010; the PEG-40 hydrogenated castor oil / decyl alcohol polyether-7 / PPG-26-butanol polyether-26 used is a composite emulsifier, commercially known as RESASSOL VS; and the polyglycerol-6 octanoate / polyglycerol-3 cocoate / polyglycerol-4 decanoate / polyglycerol-6 ricinoleate used is a composite emulsifier, commercially known as... Solve 61MB.

[0053] The extracts of clove (EUGENIA CARYOPHYLLUS) flower buds and thyme (THYMUSSERPYLLUM) used in the examples were purchased from Guangzhou GANENG Biotechnology Co., Ltd., and the extract of neem (MELIA AZADIRACHTA) seed was purchased from Yunnan Yingge Biotechnology Co., Ltd.

[0054] Example 1:

[0055] An additive with mite-repellent properties, by weight, is made from the following raw materials:

[0056] Table 1. Raw material composition in Example 1

[0057]

[0058]

[0059] The Mayinglong raw powder used in the above embodiments was obtained by air jet milling and sieving of 1.5 parts of fine pearl powder, 0.6 parts of coarse amber powder and 97.9 parts of zinc carbonate hydroxide.

[0060] This embodiment also provides a method for preparing the above-mentioned additive, comprising the following steps:

[0061] (1) Pulverize the borneol, menthol and artificial bezoar in component A into fine powder, add them together with the original powder of Mayinglong into polysorbate-20, stir and heat to 75-80 °C until completely dissolved, then cool to room temperature to obtain mixture 1 for later use;

[0062] (2) Add 4-terpineol, Litsea cubeba fruit oil, Eugenia acrylica ophyllus flower bud extract, Thymus sempyllum extract and Melia azedarach seed extract from component B to mixture 1 in step (1), stir, homogenize and mix to obtain mixture 2;

[0063] (3) Add butanediol, 1,2-hexanediol and phenoxyethanol / ethylhexylglycerol from component C to the mixture 2 obtained in step (2) and stir until homogeneous to obtain mixture 3;

[0064] (4) Finally, add deionized water to the mixture 3 obtained in step (3) and stir evenly to obtain an additive with mite-removing effect.

[0065] Example 2:

[0066] An additive with mite-repellent properties, by weight, is made from the following raw materials:

[0067] Table 2. Raw material composition in Example 2

[0068]

[0069]

[0070] The Mayinglong raw powder used in the above embodiments was obtained by air jet milling and sieving of 2.5 parts of fine pearl powder, 2 parts of coarse amber powder and 95.5 parts of zinc carbonate hydroxide.

[0071] This embodiment also provides a method for preparing the above-mentioned additive, comprising the following steps:

[0072] (1) Pulverize the borneol, menthol and artificial bezoar in component A into fine powder, add them together with the original powder of Mayinglong to polysorbate-80, stir and heat to 75-80℃, dissolve and then cool to room temperature to obtain mixture 1 for later use;

[0073] (2) Add 4-terpineol, Litsea cubeba fruit oil, Eugenia acrylica ophyllus flower bud extract, Thymus sempyllum extract and Melia azedarach seed extract from component B to mixture 1 in step (1), stir, homogenize and mix to obtain mixture 2;

[0074] (3) Add glycerol, 1,2-propanediol and phenoxyethanol / ethylhexylglycerol from component C to mixture 2 obtained in step (2) and stir until homogeneous to obtain mixture 3;

[0075] (4) Finally, add deionized water to the mixture 3 obtained in step (3) and stir evenly to obtain an additive with mite-removing effect.

[0076] Example 3:

[0077] An additive with mite-repellent properties, by weight, is made from the following raw materials:

[0078] Table 3. Raw material composition in Example 3

[0079]

[0080] The Mayinglong raw powder used in the above embodiments was obtained by air jet milling and sieving of 1.5 parts of fine pearl powder, 0.8 parts of coarse amber powder and 97.7 parts of zinc carbonate hydroxide.

[0081] This embodiment also provides a method for preparing the above-mentioned additive, comprising the following steps:

[0082] (1) Pulverize the borneol, menthol and artificial bezoar in component A into fine powder, add them together with the original powder of Mayinglong into polysorbate-20, stir and heat to 75-80℃ to dissolve, and then cool to room temperature to obtain mixture 1 for later use;

[0083] (2) Add 4-terpineol, Litsea cubeba fruit oil, Eugenia acrylica ophyllus flower bud extract, Thymus sempyllum extract and Melia azedarach seed extract from component B to mixture 1 in step (1), stir, homogenize and mix to obtain mixture 2;

[0084] (3) Add butanediol, glycerol, and phenoxyethanol / ethylhexylglycerol from component C to mixture 2 obtained in step (2) and stir until homogeneous to obtain mixture 3;

[0085] (4) Finally, add deionized water to the mixture 3 obtained in step (3) and stir evenly to obtain an additive with mite-removing effect.

[0086] Example 4:

[0087] An additive with mite-repellent properties, by weight, is made from the following raw materials:

[0088] Table 4. Raw material composition in Example 4

[0089]

[0090] The Mayinglong raw powder used in the above embodiments was prepared by air jet milling and sieving of 1 part pearl fine powder, 1 part amber coarse powder and 98 parts zinc carbonate hydroxide.

[0091] This embodiment also provides a method for preparing the above-mentioned additive, comprising the following steps:

[0092] (1) Pulverize the borneol, menthol and artificial bezoar in component A into fine powder, and add them together with the original powder of Mayinglong into the composite emulsifier PEG-40 hydrogenated castor oil / decyl polyether-7 / PPG-26-butanol polyether-26. Stir and heat to 75-80℃ to dissolve, and then cool to room temperature to obtain mixture 1 for later use.

[0093] (2) Add 4-terpineol, Litsea cubeba fruit oil, Eugenia acrylica ophyllus flower bud extract, Thymus sempyllum extract and Melia azedarach seed extract from component B to mixture 1 in step (1), stir, homogenize and mix to obtain mixture 2;

[0094] (3) Add butanediol, 1,2-hexanediol and phenoxyethanol / ethylhexylglycerol from component C to the mixture 2 obtained in step (2) and stir until homogeneous to obtain mixture 3;

[0095] (4) Finally, add deionized water to the mixture 3 obtained in step (3) and stir evenly to obtain an additive with mite-removing effect.

[0096] Example 5:

[0097] An additive with mite-repellent properties, by weight, is made from the following raw materials:

[0098] Table 5. Raw material composition in Example 5

[0099]

[0100] The Mayinglong raw powder used in the above embodiments was obtained by air jet milling and sieving of 2 parts pearl fine powder, 0.1 parts amber coarse powder and 97.9 parts zinc carbonate hydroxide.

[0101] This embodiment also provides a method for preparing the above-mentioned additive, comprising the following steps:

[0102] (1) Pulverize the borneol, menthol and artificial bezoar in component A into fine powder, and add them together with the original powder of Mayinglong into the composite emulsifier polyglycerol-6 caprylate / polyglycerol-3 cocoate / polyglycerol-4 decanoate / polyglycerol-6 ricinoleate. Stir and heat to 75-80 °C to dissolve, and then cool to room temperature to obtain mixture 1 for later use.

[0103] (2) Add 4-terpineol, Litsea cubeba fruit oil, Eugenia acrylica ophyllus flower bud extract, Thymus sempyllum extract and Melia azedarach seed extract from component B to mixture 1 in step (1), stir, homogenize and mix to obtain mixture 2;

[0104] (3) Add 1,2-propanediol, 1,2-hexanediol and phenoxyethanol / ethylhexylglycerol from component C to mixture 2 obtained in step (2) and stir until homogeneous to obtain mixture 3;

[0105] (4) Finally, add deionized water to the mixture 3 obtained in step (3) and stir evenly to obtain an additive with mite-removing effect.

[0106] Comparative Example 1:

[0107] Compared with Example 1, no Mayinglong powder was added to component A, while the dosage and preparation method of other components were the same.

[0108] Comparative Example 2:

[0109] Compared with Example 1, no artificial bezoar was added to component A, while the amounts of other components and the preparation methods were the same.

[0110] Comparative Example 3:

[0111] Compared with Example 1, no menthol was added to component A, while the amounts of other components and the preparation methods were the same.

[0112] Comparative Example 4:

[0113] Compared with Example 1, component B does not contain Melia azedarach seed extract, while the amounts of other components and the preparation methods are the same.

[0114] Application Example 1:

[0115] The additives obtained in Examples 1-5 and Comparative Examples 1-4 were evenly dripped onto the cotton pad substrate, which had been cut, sterilized, and disinfected, at a mass ratio of 2:1. The substrate was then sealed and packaged to obtain eye mite removal cotton pads.

[0116] Application Example 2:

[0117] The additives obtained in Examples 1-5 and Comparative Examples 1-4 were evenly dripped onto the wet wipe substrate, which had been cut, sterilized, and disinfected, at a mass ratio of 3:1. The substrate was then sealed and packaged to obtain eye mite-removing sanitary wipes.

[0118] Test Example 1: Eye Irritation Test of the Product

[0119] Test standard: SN / T2329-2009 "Cosmetic Eye Irritation / Corrosivity Test: Chicken Embryo Cholanosome Membrane Test"

[0120] Experimental conditions: 9-day-old SPF grade chicken embryos, 0.3 ml of sample was directly applied to the allantoic membrane CAM, the reaction was allowed to proceed for 3 min, the paste was rinsed away with 0.9% physiological saline, and vascular changes were observed after 30 s.

[0121] Experimental sample: The product obtained in Example 1.

[0122] Evaluation indicators:

[0123] Hemorrhage (H): Blood flows out of the CAM membrane vessels, which can manifest in various forms such as punctate hemorrhages or flocculent diffuse hemorrhages outside the vessels.

[0124] Coagulation (C): Denaturation of intravascular and extravascular proteins, manifested as slowed blood flow or thrombosis within the blood vessels, with the blood vessels appearing brownish-black and the extravascular area appearing cloudy and opaque.

[0125] Vascular dissolution (L): The walls of small blood vessels on the CAM membrane rupture, and the small blood vessels dissolve and disappear.

[0126] Record the severity of the three toxic endpoints (none: 0 points, mild: 1 point, moderate: 2 points, severe: 3 points). Stimulation score ES = hemorrhage (H) + coagulation (C) + angiolysis (L). Evaluate the acute eye irritation classification according to Table 4.

[0127] Table 6. Evaluation of the results of the endpoint scoring method

[0128] Finish line score Irritant Classification ES≤12 Non-irritating / mildly irritating 12 < ES < 16 moderate irritation ES≥16 Strongly irritating / corrosive

[0129] Experimental Analysis Results and Conclusions: Using the endpoint evaluation method, Figure 1 shows the comparison of chicken embryo stimulation before and after each toxicological endpoint of the product prepared in Example 1. Figure 1a The chorioallantoic membrane of the chicken embryo before the product takes effect. Figure 1b The chicken embryo chorioallantoic membrane after the product was applied showed no significant changes. Therefore, it can be concluded that the eye irritation result of the product prepared in Example 1 was no / mild irritation. The mite-removing composition of the present invention has low irritation and is especially suitable for use in the eyes.

[0130] Test Example 2: Product Mite Removal Efficacy Test

[0131] The acaricidal effects of the additives prepared in Examples 1-5 and Comparative Examples 1-4 of this invention were investigated according to the methods described in NY / T 1151.2-2006 "Indoor Efficacy Test Methods and Evaluation of Pesticide Registration Sanitary Insecticides Part 2: Acaricides and Mite Repellents". 200 mites were selected for each group. The number of dead mites was checked and recorded after 48 hours of treatment, and the acaricidal rate was calculated. The efficacy evaluation indicators for the acaricides were: Grade A (acaricidal rate = 100%) and Grade B (acaricidal rate ≥ 95%). The test results of the product samples are shown in Table 7.

[0132] Table 7. Results of the acaricide test on the additives

[0133] Sample Name Number of test mites (units) Number of dead mites (individuals) Mite eradication rate Example 1 200 200 100％ Example 2 200 200 100％ Example 3 200 200 100％ Example 4 200 200 100％ Example 5 200 200 100％ Comparative Example 1 200 175 87.5％ Comparative Example 2 200 191 95.5％ Comparative Example 3 200 188 94％ Comparative Example 4 200 181 90.5％

[0134] As shown in Table 5, the mite-killing rate of the additives prepared in the embodiments of the present invention is 100%, and all reach Grade A; the mite-killing rate of Comparative Example 2 is ≥95%, reaching Grade B; the mite-killing rate of Comparative Example 1 and Comparative Example 3-4 is <95%, and the mite-killing effect is not good.

[0135] Trial Example 3: Evaluation of Clinical Trial Use

[0136] The eye mite-removing cotton pads prepared in accordance with Case 1 were evaluated in a clinical trial for their effectiveness in removing mites and relieving itching in the eyes.

[0137] Experimental Methods: One hundred patients aged 20-50 years with Demodex blepharitis (the main clinical manifestations of Demodex blepharitis are recurrent redness of the eyelid margin, dry eyes, itching, burning sensation, foreign body sensation, photophobia, and increased secretions; severe cases may be accompanied by recurrent eyelash loss; when the inflammatory reaction involves the cornea, blurred vision or decreased visual acuity may occur) were selected. The patients were randomly divided into 5 groups of 20 each. The mite-removing cotton pads prepared with the additives prepared in Example 1 and Comparative Examples 1-4 were used respectively. The improvement of the main eye symptoms, the irritation of the product to the eyes, and the overall satisfaction with the product were recorded after 7 days of continuous use. The symptom improvement rate and overall satisfaction rate were further calculated. The experimental results are shown in Table 8.

[0138] How to use the product: Wash your hands, tear open the packaging, take out and open the cotton pad, close your eyes slightly, and wipe back and forth on the eyelids and the skin around the eyes, once in the morning and once in the evening.

[0139] Table 8. Clinical trial evaluation results of the mite-removing effect of mite-removing cotton pads on the eyes.

[0140]

[0141]

[0142] Clinical trial results show that the mite-removing cotton pads prepared in this embodiment of the invention have a good effect on improving various ocular symptoms of Demodex blepharitis. The product is non-irritating during use, and the overall satisfaction rate reaches 100%. In contrast, the comparative product also improves various ocular symptoms, but the degree of improvement is insufficient, and the overall satisfaction rate is not good. Therefore, the composition described in this invention has good mite-removing and antipruritic effects with low irritation, making it very suitable for use as an ocular mite-removing product.

[0143] The applicant declares that this invention illustrates the mite-removing composition, the mite-removing additive containing the composition, the preparation method thereof, and their applications through the above embodiments and application examples. However, this invention is not limited to the above embodiments, meaning that this invention does not necessarily rely on the above embodiments for implementation. Those skilled in the art should understand that any improvements to this invention, equivalent substitutions of individual raw materials in the product of this invention, addition of auxiliary components, and selection of specific methods, all fall within the protection and disclosure scope of this invention.

Claims

1. The application of a mite-removing additive in the preparation of eye mite-removing products, characterized in that, The mite-removing additive is prepared from the following raw materials in parts by weight: ； And 1-80 parts of polyol, 0.1-20 parts of emulsifier, 0.1-2 parts of preservative, and 10-95 parts of deionized water; The original powder of Mayinglong is a mixture of zinc carbonate hydroxide, amber powder and pearl powder, with a dosage ratio of (80~100):(0.1~2):(0.1~2).

2. The application as described in claim 1, characterized in that, The polyol is selected from at least one of glycerol, 1,2-propanediol, 1,3-propanediol, butylene glycol, sorbitol, dipropylene glycol, pentanediol, decanediol, octanediol, and octanediol.

3. The application as described in claim 1, characterized in that, The emulsifier is selected from at least one of polysorbate-20, polysorbate-80, PEG-40 hydrogenated castor oil / decyl alcohol polyether-7 / PPG-26-butanol polyether-26, polyglycerol-6 caprylate / polyglycerol-3 cocoate / polyglycerol-4 decanoate / polyglycerol-6 ricinoleate.

4. The application as described in claim 3, characterized in that, The preservative is selected from at least one of phenoxyethanol, ethylhexylglycerin, chlorphenesin, methylparaben, p-hydroxyacetophenone, and 1,2-hexanediol.

5. The application as described in any one of claims 1-4, characterized in that, The preparation method of the mite-removing additive includes the following steps: (1) Pulverize borneol, menthol and artificial bezoar into fine powder, add them together with Mayinglong raw powder into emulsifier, mix and stir and heat, and after complete dissolution, cool down to obtain the first mixture; (2) Add 4-terpineol, Litsea cubeba fruit oil, Eugenia caryopsis flower bud extract, Thymus sempylum extract, and Melia azadiracta seed extract to the first mixture in step (1) and mix well to obtain the second mixture; (3) Add the polyol and preservative to the second mixture obtained in step (2) and stir until homogeneous to obtain the third mixture; (4) Add deionized water to the third mixture obtained in step (3) and stir evenly to obtain the mite-removing additive.

6. The use of the mite-removing additive as described in any one of claims 1-4 in the preparation of cosmetics, disinfection products or daily necessities with mite-removing effects.

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