Application of aromatic carboxamide cyclopropionic acid compounds in promoting rice growth and development
By using seed coating agents, seed mixing agents or coated rice seeds of arboxamide cyclopropionic acid compounds, the problem of low effective tiller counts and stem tillering rate is solved, and the rice yield and growth safety are improved, and the treatment steps are simplified.
Patent Information
- Application Number
- CN202310569232.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2023-05-19
- Publication Date
- 2025-06-06
- Estimated Expiration
- 2043-05-19
AI Technical Summary
In the prior art, the effective tiller number of rice is not high, and the stem tiller rate is low, which affects the final yield of rice. There are side effects of traditional hormone treatment, which affects the safety of rice growth.
Araloamide cyclopropionic acid compounds are used as seed coating agents, seed mixing agents or coated rice seeds. Through seed soaking and foliar spraying, the germination of rice seeds, the growth and development of seedlings and seedlings, especially effective tillering and stem-turning rate.
Increase the effective tiller count of rice, increase the spike type, increase yield, and do not negatively affect rice growth, simplify the treatment steps, and are suitable for different regions and rice varieties.
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Abstract
Description
Technical Field
[0001] The invention relates to application of aromatic carboxamide cyclopropionic acid compounds in promoting the growth and development of rice, and belongs to the technical field of crop production. Background Art
[0002] Tillering is one of the most important agronomic traits of rice. Rice tillering is divided into two categories: effective tillering and ineffective tillering. In high-yield populations, the presence of ineffective tillers will affect the normal development of effective tillers and the photosynthesis of the population in the later stage. Inhibiting the occurrence of ineffective tillers and increasing the tillering rate of the population are important prerequisites for the formation of a reasonable rice population and thus high yields. Rice tillering is formed by the activation of axillary buds in the axils of leaves, which is similar to the formation of plant branches and is controlled by apical growth advantage. Apical growth advantage is regulated by a type of inhibitory auxin, which is produced at the top (such as the heart leaf of rice) and transported to the base in the stem, affecting the growth of axillary buds. Based on the relationship between apical growth advantage and axillary bud growth, in recent years, many researchers have applied paclobutrazol and linconazole in the rice seedling field to weaken the apical growth advantage of plants, promote the growth of lateral branches, and cultivate strong rice seedlings; gibberellins are applied at the end of effective tillering of rice to enhance the apical growth advantage of plants, inhibit the birth of tillers, reduce ineffective tillers, and promote the enlargement of main stems and tillers and the increase of panicle shape. However, the application of such hormones that promote apical growth will produce many side effects while promoting or inhibiting the occurrence of rice tillers, such as lengthening or shortening of internodes, leaves, and leaf sheaths, and changing the growth process of rice, which has an adverse effect on the safe production of rice.
[0003] At present, the tillering rate of rice in large-scale production in China is only 60% to 70%, while the tillering rate of super-high-yield populations must reach more than 80%. Promoting effective tillering can increase the tillering rate, make each effective stem develop more fully, increase the panicle shape, and achieve a greater breakthrough in yield. Therefore, how to safely and effectively control the ineffective tillering of rice and promote effective tillering of rice to increase yield without affecting the normal growth of rice has become a difficult point in the research and application of rice chemical control technology. Summary of the invention
[0004] The purpose of the present invention is to provide an application of an aromatic carboxamide cyclopropionic acid compound in promoting the growth and development of rice, so as to solve the problem in the prior art that the number of effective tillers of rice is not high, the tillering rate is low, and thus the final yield of rice is affected.
[0005] The second object of the present invention is to provide the use of aromatic carboxamide cyclopropionic acid compounds in seed dressing agents, seed dressing agents or coated rice seeds. These products are easy to use and reduce the professional requirements for users.
[0006] In order to achieve the above purpose, the first technical solution of the present invention is:
[0007] Application of aromatic carboxamidocyclopropionic acid compounds in promoting the growth and development of rice, wherein the aromatic carboxamidocyclopropionic acid compounds are one or more of 2,4-dichlorobenzamidocyclopropionic acid, 2-hydroxyphenylamidocyclopropionic acid, 4-chlorophenylcarboxamidocyclopropionic acid, 2-chlorophenylamidocyclopropionic acid, 3-nitrophenylamidocyclopropionic acid, and 4-nitrophenylamidocyclopropionic acid; and the promotion of rice growth and development is the promotion of seed germination, seedling growth and development, or rice seedling growth and development.
[0008] The present invention uses aromatic carboxamide cyclopropionic acid compounds for the growth and development of rice, which can not only promote seed germination and seedling growth and development, but also play a key role in the growth and development of rice seedlings, especially can promote effective tillering of rice and tillering rate, and finally improve the yield of rice. Moreover, the substance has no negative impact on rice growth and no pollution to the environment, and can be widely used in the planting and production of different rice varieties in different regions.
[0009] In order to better observe the effects of aromatic carboxamide cyclopropionic acid compounds on rice seed germination and seedling growth and development, preferably, promoting seed germination and seedling growth and development is achieved by seed soaking treatment.
[0010] The seedlings refer to rice seedlings in the period from seed germination to three leaves and one heart, including the three leaves and one heart period.
[0011] Preferably, the concentration of the agent for seed soaking treatment is 0.1-1 mg / L during seed soaking. The agent at this concentration can better promote seed germination and seedling growth and development.
[0012] When the aromatic carboxamidocyclopropionic acid compound is 2,4-dichlorobenzamidocyclopropionic acid, the concentration is preferably 0.15 to 0.6 mg / L, and more preferably 0.3 mg / L.
[0013] When the aromatic carboxamidocyclopropionic acid compound is 2-hydroxyphenylamidocyclopropionic acid, the concentration is preferably 0.25 to 1 mg / L, more preferably 0.5 mg / L.
[0014] When the aromatic carboxamidocyclopropionic acid compound is 4-chlorophenylcarboxamidocyclopropionic acid, the concentration is preferably 0.4 to 0.8 mg / L, and more preferably 0.6 to 0.8 mg / L.
[0015] When the aromatic carboxamidocyclopropionic acid compound is 2-chlorophenylamidocyclopropionic acid, the concentration is preferably 0.4 to 0.8 mg / L, more preferably 0.6 mg / L.
[0016] When the aromatic carboxamidocyclopropionic acid compound is 3-nitrophenylamidocyclopropionic acid, the concentration is preferably 0.125 to 1.0 mg / L, more preferably 0.25 mg / L.
[0017] When the aromatic carboxamidocyclopropionic acid compound is 4-nitrophenylamidocyclopropionic acid, the concentration is preferably 0.125 to 0.25 mg / L, more preferably 0.25 mg / L.
[0018] Preferably, the active ingredient of the reagent used for the seed soaking treatment is an aromatic carboxamide cyclopropionic acid compound. The reagent can be a commercially available liquid containing an aromatic carboxamide cyclopropionic acid compound.
[0019] Preferably, the seed germination promotion is to increase one or more of germination potential, germination rate, bud length, and root length. Aromatic carboxamide cyclopropionic acid compounds can increase the germination potential and germination rate of seeds, promote the growth of buds and roots, and thus increase the bud length and root length.
[0020] Preferably, promoting the growth and development of seedlings is one or more of inhibiting the excessive growth of seedlings, strengthening seedlings, and increasing dry matter accumulation. Inhibiting the excessive growth of seedlings refers to reducing plant height. Strengthening seedlings refers to increasing the width and fullness of the stem base of seedlings. Increasing dry matter accumulation refers to increasing the dry matter accumulation of the aboveground and underground parts, laying a material foundation for greening after transplanting and increasing yield. Dry matter accumulation is reflected in increasing the fresh weight and dry weight of seedlings.
[0021] In order to make the seedlings come into effective contact with the aromatic carboxamidocyclopropionic acid compounds, preferably, promoting the growth and development of the seedlings is achieved by foliar spraying.
[0022] Preferably, the active ingredient of the foliar spraying agent is an aromatic carboxamide cyclopropionic acid compound; the concentration of the agent when sprayed on the leaves is 1.0 to 20 mg / L. At this concentration, the agent can better promote the growth and development of seedlings. Preferably, it is 2.5 to 10 mg / L, and more preferably 5 mg / L.
[0023] More preferably, it is 2,4-dichlorobenzamidocyclopropionic acid.
[0024] Preferably, the foliar spraying is carried out 5 days after rice transplanting and / or 2-3 days before rice breaking. During this period, foliar spraying can more effectively promote effective tillering of the seedlings and increase yield.
[0025] Preferably, the promoting the growth and development of the rice seedlings is one or more of promoting tillering, ear formation, fruiting, growth progress, and increasing yield. These factors can comprehensively reflect the entire growth and development status of the rice seedlings.
[0026] Among them, the important indicators of tillering are the number of effective tillers and the rate of tillering per diameter. The important indicators of ear formation are the number of ears and ear length. The important indicator of fruiting is the fruiting rate, which also refers to the number of filled grains. The growth process includes the time of greening and the time of maturity. Aromatic carboxamide cyclopropionic acid compounds shorten the time of greening and maturity of rice, thereby promoting early harvest of rice and shortening the planting cycle.
[0027] The second technical solution of the present invention is:
[0028] The invention discloses an application of an aromatic carboxamide cyclopropionic acid compound in a seed dressing agent, a seed dressing agent or a coated rice seed, wherein the aromatic carboxamide cyclopropionic acid compound is one or more of 2,4-dichlorobenzamide cyclopropionic acid, 2-hydroxyphenylamide cyclopropionic acid, 4-chlorophenylcarboxamide cyclopropionic acid, 2-chlorophenylamide cyclopropionic acid, 3-nitrophenylamide cyclopropionic acid and 4-nitrophenylamide cyclopropionic acid.
[0029] Preferably, the active ingredient of the seed coating agent is an aromatic carboxamide cyclopropionic acid compound. The seed coating agent is obtained by processing aromatic carboxamide cyclopropionic acid with a carrier, solvent and adjuvant commonly used in agriculture.
[0030] Preferably, the seed dressing agent is obtained by mixing aromatic carboxamide cyclopropionic acid with a commercially available seed dressing agent in a certain proportion.
[0031] Preferably, the coated rice seeds are made of aromatic carboxamidocyclopropionic acid, adjuvants, auxiliary materials and seeds.
[0032] More preferably, the auxiliary agents and adjuvants are adhesives, wetting agents, film formers and colorants commonly used in commercial seed coating agents. The solvent is water.
[0033] The seed coating agent, seed dressing agent and coated rice seeds provided by the invention can be directly sown or used as seed dressing, avoiding the need to soak the rice seeds with chemicals before sowing, thus simplifying the processing steps.
[0034] Preferably, the amount of the aromatic carboxamide cyclopropionic acid compound in the seed dressing agent, seed dressing agent or coated rice seeds is 1-5 g / 100 kg of seeds. DETAILED DESCRIPTION
[0035] The formula composition of the 2,4-dichlorobenzamidocyclopropionic acid soluble solution in the following examples is: 1.2% of 2,4-dichlorobenzamidocyclopropionic acid, 4-6% of antifreeze, 2.5% or 5% of surfactant, 0.1-0.2% of defoamer, 0.05-0.2% of pH regulator, and the balance is water. Among them, the antifreeze agent is selected from ethylene glycol, propylene glycol or glycerol; the surfactant is selected from Tween80, OP-10 or APG1214; the defoamer is selected from organic siloxane, silicone or tributyl phosphate; the pH regulator is selected from formic acid, acetic acid or ethanolamine. The specific preparation method adopts the method of Example 8 in the specification of the Chinese invention patent with the authorization announcement number CN113317322B.
[0036] The formula composition of the 2-hydroxyphenylamide cyclopropionic acid soluble solution is: 1.2% of 2-hydroxyphenylamide cyclopropionic acid, 4-6% of antifreeze, 5% of surfactant, 0.1-0.2% of defoamer, 0.05-0.2% of pH regulator, and the balance is water; the antifreeze is selected from propylene glycol or glycerol; the surfactant is selected from OP-10 or APG1214; the defoamer is selected from organic siloxane, silicone or tributyl phosphate; the pH regulator is selected from citric acid, acetic acid or ethanolamine. The specific preparation method adopts the method of Example 9 in the specification of the Chinese invention patent with the authorization announcement number of CN113317322B for preparation.
[0037] The formula composition of the soluble solution of 4-chlorophenylcarboxamidocyclopropionic acid is: 1% 4-chlorophenylcarboxamidocyclopropionic acid, 0.2% choline chloride, 4-6% antifreeze, 6% surfactant, 0.1-0.2% defoamer, 0.05-0.2% pH regulator, and the balance is water; the antifreeze is selected from propylene glycol or glycerol; the surfactant is selected from AEO-25, OP-10 or APG1214; the defoamer is selected from organic siloxane, silicone or tributyl phosphate; the pH regulator is selected from formic acid, acetic acid or citric acid. The specific preparation method adopts the method of Example 10 in the specification of the Chinese invention patent with the authorization announcement number of CN113317322B for preparation.
[0038] The formula composition of the 2-chlorophenylamidocyclopropionic acid soluble solution is: 2% 2-chlorophenylamidocyclopropionic acid, 72% gibberellic acid A4+, 4-6% antifreeze, 5% surfactant, 0.1-0.2% defoamer, 0.05-0.2% pH regulator, 20% N,N-dimethyldecylamide, and the balance is water; the antifreeze is selected from ethylene glycol or propylene glycol; the surfactant is selected from AEO-25 or OP-10; the defoamer is selected from organic siloxane, silicone or tributyl phosphate; the pH regulator is selected from formic acid. The specific preparation method adopts the method of Example 11 in the specification of the Chinese invention patent with the authorization announcement number of CN113317322B for preparation.
[0039] The formula composition of the 3-nitrophenyl amido cyclopropionic acid soluble solution is: 0.2% of 3-nitrophenyl amido cyclopropionic acid, 4% of aminoethyl hexanoate, 4-8% of antifreeze, 6% of surfactant, 0.1-0.2% of defoamer, and the balance is water; the antifreeze is selected from ethylene glycol or propylene glycol; the surfactant is selected from APG1214 or Tween80; the defoamer is selected from organic siloxane, silicone or tributyl phosphate. The specific preparation method adopts the method of Example 12 in the specification of the Chinese invention patent with the authorization announcement number of CN113317322B.
[0040] The formula composition of the 4-nitrophenyl amido cyclopropionic acid soluble solution is: 2% 4-nitrophenyl amido cyclopropionic acid, 5-10% antifreeze, 6-10% surfactant, 0.1-0.2% defoamer, 0.05-0.2% pH regulator, and the balance is water; the antifreeze is selected from propylene glycol; the surfactant is selected from Tween80, OP-10, AEO-25 or APG1214; the defoamer is selected from organic siloxane, silicone or tributyl phosphate; the pH regulator is selected from formic acid. The specific preparation method adopts the method of Example 13 in the specification of the Chinese invention patent with the authorization announcement number of CN113317322B.
[0041] The technical solution of the present invention is further described below in conjunction with specific implementation methods.
[0042] 1. The specific examples of the application of the aromatic carboxamide cyclopropionic acid compounds of the present invention in the growth and development of rice are as follows:
[0043] Example 1
[0044] The application of this embodiment is the effect of 2,4-dichlorobenzamidocyclopropionic acid on the development of rice seeds.
[0045] Preparation of test solution: 1.2% 2,4-dichlorobenzamidocyclopropionic acid soluble solution was diluted with ultrapure water to three concentrations of 0.15 mg / L, 0.3 mg / L, and 0.6 mg / L, and water was used as a blank control.
[0046] Test varieties: Yunonggeng 11 and Guangliangyou 419.
[0047] Test method:
[0048] (1) The seeds of the test variety were soaked and disinfected in 75% ethanol for 15 min, and then washed with ultrapure water for 5 times to obtain the disinfected rice seeds;
[0049] (2) Rice seeds were immersed in three different concentrations of 2,4-dichlorobenzamidocyclopropionic acid solutions (i.e., test groups) of 0.15 mg / L, 0.3 mg / L, and 0.6 mg / L, respectively, and in a blank control (ultrapure water) for 24 h;
[0050] (3) Place filter paper in the germination box, add 10 ml of ultrapure water to keep the filter paper moist, select 50 seeds from each experimental group and control group soaked in step (2) and place them on the filter paper in different germination boxes, and repeat three times;
[0051] (4) Place the germination box in a constant temperature incubator at a temperature of (25±1)°C, a relative humidity of 60%, and a 12-h light / 12-h dark period. Observe the germination and add water every 24 hours.
[0052] The germination potential was counted on the third day of culture, and the germination rate was counted on the seventh day of culture, and the data of the shoot length and root length were measured. The results are shown in Table 1.
[0053] Germination rate (%) = number of normal germinated seeds / number of test seeds × 100%
[0054] Table 1 Investigation on rice seed germination and seedling growth indicators after soaking
[0055]
[0056]
[0057] As can be seen from Table 1, the two rice varieties were soaked in different concentrations of 2,4-dichlorobenzamidocyclopropionic acid for 24 hours, and the seed germination potential, germination rate and seedling growth were promoted compared with the blank control. Among them, the 0.3 mg / L concentration treatment had a significant promoting effect on the seed germination and seedling quality of the two rice varieties, and the effect of 2,4-dichlorobenzamidocyclopropionic acid on the Guangliangyou 419 variety was more obvious, indicating that soaking rice seeds with 2,4-dichlorobenzamidocyclopropionic acid can significantly improve the germination rate and seedling quality of rice seeds.
[0058] Example 2
[0059] The application of this embodiment is the effect of 2,4-dichlorobenzamidocyclopropionic acid on the quality of rice seedlings.
[0060] Test method: The rice seeds were treated with the seed soaking concentration and seed soaking method of Example 1, and 50 seeds with uniform germination (based on seed skin breaking) after treatment were selected for potted cultivation (specifications 26.5 cm×21 cm×17.5 cm), 50 seeds were sown in each pot, five replicates were given for each treatment, and the potted plants were placed in a walk-in artificial climate chamber of Henan Crop Chemical Control Engineering Technology Research Center for cultivation.
[0061] When 80% of the seedlings reached 3 leaves and 1 heart, the seedling quality was investigated. Ten seedlings were randomly selected from each pot, and a total of 50 seedlings were selected for each treatment. Seedling biomass accumulation When 80% of the seedlings reached 3 leaves and 1 heart, 10 seedlings were randomly selected from each pot, and a total of 50 seedlings were selected for each treatment to investigate the seedling biomass. The dry and fresh weights of the aboveground and underground parts were measured respectively. The results are shown in Table 2.
[0062] Table 2 Survey form for quality of soaked rice seedlings
[0063]
[0064] As shown in Table 2, after the seed soaking treatment with 2,4-dichlorobenzamide-cyclopropionic acid, the height of the seedlings was reduced compared with the blank control, which controlled the leggy growth of the seedlings; the stem base width and fullness are one of the important indicators to describe the quality of rice seedlings. The stem base width and fullness of the rice seedlings after the seed soaking treatment are better than the blank CK control. Among them, the 0.3 mg / L concentration treatment performed best in the two rice varieties, and the stem base width and fullness data were the best, indicating that the 2,4-dichlorobenzamide-cyclopropionic acid seed soaking rice treatment has a strong seedling effect. The dry matter accumulation most directly reflects the growth potential and growth of the seedlings. After the 2,4-dichlorobenzamide-cyclopropionic acid treatment, the dry matter accumulation of the aboveground and underground parts of the seedlings increased, which laid a material foundation for returning to green after transplanting and increasing yield. Therefore, the 2,4-dichlorobenzamide-cyclopropionic acid seed soaking treatment has a significant improvement in the quality of rice seedlings.
[0065] Example 3
[0066] The application of this embodiment is the effect of 2,4-dichlorobenzamidocyclopropionic acid on the yield of rice seedlings.
[0067] Test variety: Huang Jinqing.
[0068] Experimental location: Yuanyang County, Xinxiang City, Henan Province.
[0069] Experimental method: 1.2% 2,4-dichlorobenzamidocyclopropionic acid soluble solution was diluted into three concentrations of 2.5 mg / L, 5 mg / L and 10 mg / L respectively, and water was used as a blank control. The solution was evenly sprayed on the leaves 5 days after rice transplanting and 2-3 days before rice breaking. The growth process of rice seedlings and final harvest data were tracked and investigated. The results are shown in Tables 3 and 4.
[0070] Table 3 Questionnaire on rice growth process
[0071]
[0072] It can be seen from Table 3 that, compared with the control group, spraying 2,4-dichlorobenzamidocyclopropionic acid twice during the whole growth period of rice can shorten the time for rice to turn green and mature, and promote early harvest of rice.
[0073] Table 4 Indoor test questionnaire
[0074]
[0075] It can be seen from Table 4 that after spraying 2,4-dichlorobenzamidocyclopropionic acid solution on the leaves at two periods during the whole growth period of rice, the number of effective tillers, number of effective panicles, panicle length, panicle rate, number of filled grains, 1000-grain weight and yield data of rice were significantly improved compared with the CK blank control, thereby increasing the final yield of rice.
[0076] Example 4
[0077] The application of this embodiment is the effect of 2-hydroxyphenylamidocyclopropionic acid on the development of rice seeds.
[0078] Preparation of test solution: 1.2% 2-hydroxyphenylamidocyclopropionic acid soluble solution was diluted with ultrapure water to four concentrations of 0.125 mg / L, 0.25 mg / L, 0.5 mg / L, and 1 mg / L, and water was used as a blank control.
[0079] Test variety: Zhuangxiangyou 1205
[0080] The test method is the same as that of Example 1, and the results are shown in Table 5.
[0081] Table 5 Investigation on rice seed germination and seedling growth indexes after soaking
[0082]
[0083] As shown in Table 5, the rice varieties of Zhuangxiangyou 1205 were soaked in different concentrations of 2-hydroxyphenylamidocyclopropionic acid for 24 hours. Under the treatment with higher concentrations (0.25 mg / L, 0.5 mg / L, 1 mg / L), the germination potential, germination rate and seedling growth of the seeds were significantly promoted compared with the blank control. The treatment with 0.125 mg / L concentration promoted the germination of rice seeds and the growth of seedlings. Among them, the comprehensive data of the treatment with 0.5 mg / L concentration was the best, indicating that the soaking treatment of rice with 2-hydroxyphenylamidocyclopropionic acid can significantly improve the germination rate of rice seeds and the quality of seedlings.
[0084] Example 5
[0085] The application of this embodiment is the effect of 4-chlorophenylcarboxamidocyclopropionic acid on the development of rice seeds.
[0086] Preparation of test solution: 1% 4-chlorophenylcarboxamidocyclopropionic acid soluble solution was diluted with ultrapure water to four concentrations of 0.2 mg / L, 0.4 mg / L, 0.6 mg / L and 0.8 mg / L, and water was used as a blank control.
[0087] Test variety: Zhongzheyou No. 8
[0088] The test method is the same as that in Example 1, and the results are shown in Table 6.
[0089] Table 6 Investigation on rice seed germination and seedling growth indexes after soaking
[0090]
[0091]
[0092] As shown in Table 6, different concentrations of 4-chlorophenylcarboxamidocyclopropionic acid were used to soak the rice seeds of Zhongzheyou No. 8 for 24 hours. The high concentrations of 0.6 and 0.8 mg / L treatments can significantly improve the germination potential of rice seeds and promote early germination of seeds, but have no significant promoting effect on the final germination rate of seeds and seedling growth.
[0093] Example 6
[0094] The application of this embodiment is the effect of 2-chlorophenylamidocyclopropionic acid on the development of rice seeds.
[0095] Preparation of test solution: 2% 2-chlorophenylamidocyclopropionic acid soluble solution was diluted with ultrapure water to four concentrations of 0.2 mg / L, 0.4 mg / L, 0.6 mg / L and 0.8 mg / L, and water was used as a blank control.
[0096] Test variety: Tianxiangyou 2115
[0097] The test method is the same as that in Example 1, and the results are shown in Table 7.
[0098] Table 7 Investigation on rice seed germination and seedling growth indexes after soaking
[0099]
[0100] As can be seen from Table 7, when the rice varieties of Tianxiangyou 2115 were soaked for 24 hours with different concentrations of 2-chlorophenylamidocyclopropionic acid, the germination potential and germination rate of the seeds were significantly improved compared with the blank control, among which the 0.6 mg / L treatment had the best effect; there was no significant promoting effect on the growth of the seedlings, indicating that 2-chlorophenylamidocyclopropionic acid can play a significant role in promoting seed germination.
[0101] Example 7
[0102] The application of this embodiment is the effect of 3-nitrophenylamidocyclopropionic acid on the development of rice seeds.
[0103] Preparation of test solution: 0.2% 3-nitrophenylamidocyclopropionic acid soluble solution was diluted with ultrapure water to four concentrations of 0.125 mg / L, 0.25 mg / L, 0.5 mg / L and 1 mg / L, and water was used as a blank control.
[0104] Test variety: Pengyou 1269
[0105] The test method is the same as that in Example 1, and the results are shown in Table 8.
[0106] Table 8 Survey on rice seed germination and seedling growth indicators after soaking
[0107]
[0108] As can be seen from Table 8, the rice varieties of Pengyou 1269 were soaked in different concentrations of 3-nitrophenylamidocyclopropionic acid for 24 hours. The growth of rice seedlings was significantly promoted compared with the blank control. The sprout length and root length data were significantly different from those of the blank control, among which the concentration of 0.25 mg / L was the best. There was no significant promoting effect on the germination potential and germination rate of rice seeds in the germination stage, indicating that the treatment of rice seeds soaked in 3-nitrophenylamidocyclopropionic acid can significantly improve the quality of rice seedlings after germination.
[0109] Example 8
[0110] The application of this embodiment is the effect of 4-nitrophenylamidocyclopropionic acid on the development of rice seeds.
[0111] Preparation of test solution: 2% 4-nitrophenylamidocyclopropionic acid soluble solution was diluted with ultrapure water to four concentrations of 0.125 mg / L, 0.25 mg / L, 0.5 mg / L, and 1 mg / L, and water was used as a blank control.
[0112] Test variety: Shenliangyou 5814
[0113] The test method is the same as that of Example 1, and the results are shown in Table 9.
[0114] Table 9 Investigation on rice seed germination and seedling growth indexes after soaking
[0115]
[0116] As can be seen from Table 9, the rice varieties of Shenliangyou 5814 were soaked in different concentrations of 4-nitrophenylamidocyclopropionic acid for 24 hours. The seed germination potential and germination rate were significantly promoted compared with the blank control. The low concentration (0.125 mg / L, 0.25 mg / L) treatment had a better effect, among which the 0.25 mg / L treatment was the best. There was no obvious promoting effect on the growth of seedlings. This shows that soaking rice seeds with 4-nitrophenylamidocyclopropionic acid can promote seed germination speed and improve seed germination rate.
[0117] 2. The specific implementation methods of the use of the aromatic carboxamide cyclopropionic acid compounds of the present invention in seed coating agents, seed dressing agents or coated rice seeds are as follows:
[0118] Example 9
[0119] In this embodiment, aromatic carboxamide cyclopropionic acid is used as an effective ingredient of a seed coating agent, and aromatic carboxamide cyclopropionic acid is processed into a seed coating agent with a carrier, a solvent and an auxiliary agent conventionally used in agriculture. The aromatic carboxamide cyclopropionic acid is one of 2,4-dichlorobenzamide cyclopropionic acid, 2-hydroxyphenylamide cyclopropionic acid, 4-chlorophenylcarboxamide cyclopropionic acid, 2-chlorophenylamide cyclopropionic acid, 3-nitrophenylamide cyclopropionic acid and 4-nitrophenylamide cyclopropionic acid.
[0120] Example 10
[0121] In this embodiment, aromatic carboxamide cyclopropionic acid is used as an effective ingredient of a seed dressing agent, wherein aromatic carboxamide cyclopropionic acid is mixed with a finished seed dressing agent in a certain proportion and then used for seed dressing. The aromatic carboxamide cyclopropionic acid is one of 2,4-dichlorobenzamide cyclopropionic acid, 2-hydroxyphenylamide cyclopropionic acid, 4-chlorophenylcarboxamide cyclopropionic acid, 2-chlorophenylamide cyclopropionic acid, 3-nitrophenylamide cyclopropionic acid, and 4-nitrophenylamide cyclopropionic acid.
[0122] Embodiment 11
[0123] The coated rice seeds of this embodiment are prepared by conventional coating means, by adding various adjuvants and auxiliary materials and seeds to aromatic carboxamidocyclopropionic acid, and are composed of the following components: aromatic carboxamidocyclopropionic acid, a binder, a wetting agent, a film-forming agent, a colorant, water and rice seeds, wherein the aromatic carboxamidocyclopropionic acid is one of 2,4-dichlorobenzamidocyclopropionic acid, 2-hydroxyphenylamidocyclopropionic acid, 4-chlorophenylcarboxamidocyclopropionic acid, 2-chlorophenylamidocyclopropionic acid, 3-nitrophenylamidocyclopropionic acid and 4-nitrophenylamidocyclopropionic acid, and the binder, wetting agent, film-forming agent and colorant can be one or more of the commonly used seed coating agent raw materials on the market.
[0124] In the application of Examples 9-11, the recommended dosage of 2,4-dichlorobenzamidocyclopropionic acid is 1-2 g / 100 kg of seeds; the recommended dosage of 4-chlorophenylcarboxamidocyclopropionic acid is 3-5 g / 100 kg of seeds; the recommended dosage of 2-hydroxyphenylamidocyclopropionic acid is 2-4 g / 100 kg of seeds; the recommended dosage of 2-chlorophenylamidocyclopropionic acid is 2-3 g / 100 kg of seeds; the recommended dosage of 3-nitrophenylamidocyclopropionic acid is 2-4 g / 100 kg of seeds; and the recommended dosage of 4-nitrophenylamidocyclopropionic acid is 1-2 g / 100 kg of seeds.
Claims
1. Application of aromatic carboxamide cyclopropionic acid compounds in promoting rice growth and development, It is characterized in that The aromatic carboxamide cyclopropionic acid compound is one or more of 2,4-dichlorobenzamide cyclopropionic acid, 2-hydroxyphenylamide cyclopropionic acid, 4-chlorophenylcarboxamide cyclopropionic acid, 2-chlorophenylamide cyclopropionic acid, 3-nitrophenylamide cyclopropionic acid, and 4-nitrophenylamide cyclopropionic acid; the promoting rice growth and development is promoting seed germination or seedling growth and development; the promoting seed germination and seedling growth and development is achieved by seed soaking treatment, and the concentration of the seed soaking treatment agent during seed soaking is 0.1-1 mg / L.
2. The use of the aromatic carboxamide cyclopropionic acid compound according to claim 1 in promoting the growth and development of rice, It is characterized in that The promoting of seed germination is to increase one or more of germination potential, germination rate, sprout length and root length.
3. The use of the aromatic carboxamide cyclopropionic acid compound according to claim 1 in promoting the growth and development of rice, It is characterized in that Promoting the growth and development of seedlings is one or more of inhibiting the excessive growth of seedlings, strengthening seedlings, and increasing dry matter accumulation.
4. Application of aromatic carboxamide cyclopropionic acid compounds in promoting rice growth and development, It is characterized in that The aromatic carboxamide cyclopropionic acid compound is one or more of 2,4-dichlorobenzamide cyclopropionic acid, 2-hydroxyphenylamide cyclopropionic acid, 4-chlorophenylcarboxamide cyclopropionic acid, 2-chlorophenylamide cyclopropionic acid, 3-nitrophenylamide cyclopropionic acid, and 4-nitrophenylamide cyclopropionic acid; the promoting rice growth and development is promoting the growth and development of seedlings; promoting the growth and development of seedlings is achieved by foliar spraying; the active ingredient of the foliar spraying reagent is an aromatic carboxamide cyclopropionic acid compound; the concentration of the reagent when sprayed on the leaves is 1.0-20 mg / L.
5. Use of the aromatic carboxamide cyclopropionic acid compound according to claim 4 in promoting the growth and development of rice, It is characterized in that The time for foliar spraying is 5 days after rice transplanting and / or 2-3 days before rice breaking.
6. Application of aromatic carboxamide cyclopropionic acid compounds in seed dressing agents, seed dressing agents or coated rice seeds, It is characterized in that The application is to promote seed germination or seedling growth and development, and the dosage of the aromatic carboxamide cyclopropionic acid compound in the seed dressing agent, seed dressing agent or coated rice seeds is 1-5g / 100kg of seeds; the aromatic carboxamide cyclopropionic acid compound is one or more of 2,4-dichlorobenzamide cyclopropionic acid, 2-hydroxyphenylamide cyclopropionic acid, 4-chlorophenylcarboxamide cyclopropionic acid, 2-chlorophenylamide cyclopropionic acid, 3-nitrophenylamide cyclopropionic acid, and 4-nitrophenylamide cyclopropionic acid.
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