A fruit coloring composition, a fruit coloring functional fertilizer and applications thereof

Abstract

The present invention belongs to the field of plant growth regulators, and specifically relates to a fruit coloring composition, a fruit coloring functional fertilizer and their applications. The fruit coloring composition comprises 1-aminocyclopropane carboxylic acid and a protective auxiliary agent, and the mass ratio of 1-aminocyclopropane carboxylic acid to the protective auxiliary agent is 1:0.1 to 200; the protective auxiliary agent is selected from 5-aminolevulinic acid, diethyl aminoethyl hexanoate or choline chloride. The present invention first discloses the use of protective auxiliary agents such as 5-aminolevulinic acid, which can effectively improve the adverse effects when ACC is applied alone, such as high dosage for coloring, easy to cause negative phenomena such as plant defoliation, fruit cracking, fruit drop and quality decline. When the effective application dosage is only 0.05 to 2.5 ppm, excellent coloring effect can be achieved. At the same time, the safety of exogenous application of ACC is effectively improved, and the planting effect of fruit and vegetable crops can be improved in aspects such as preventing fruit drop, increasing fruit sweetness, reducing phytotoxicity, and relieving the growth inhibition of stems, leaves and fruits.

Description

Technical Field

[0001] The present invention belongs to the field of plant growth regulators, and specifically relates to a fruit coloring composition, a fruit coloring functional fertilizer and their applications. Background Art

[0002] 1-Aminocyclopropane-1-carboxylic acid, abbreviated as ACC, was discovered in 1979 to be the direct precursor for the synthesis of endogenous ethylene in plants. Its biosynthetic process is: methionine - S-adenosylmethionine - ACC - ethylene. Methionine is first converted to S-adenosylmethionine, which is catalyzed by ACC synthase to generate ACC, and ACC is then oxidized by ACC oxidase to generate ethylene. When ACC is applied to plants, it exhibits the characteristic "triple response" of ethylene. Exogenous supplementation of ACC is an effective way to increase the concentration of ethylene in plants and can replace the function of ethephon, a widely used ethylene-releasing agent.

[0003] Sumitomo of Japan has registered ACC for use in thinning fruits and increasing flower return in apple trees and nuts, with the effective ingredient dosage being 100 - 600 mg / L. The Chinese patent application with publication number CN114989024A discloses the applications of 1-aminocyclopropane-1-carboxylic acid in promoting the ripening and coloring of Crimson Seedless grapes, promoting the yellowing and ripening of tobacco, increasing the ratio of male and female flowers in cucumbers, promoting root growth of zucchini seedlings, and thinning flowers and fruits. Experiments show that when the effective concentration range of ACC is 300 - 500 ppm, it can promote the rapid ripening and color change of Crimson Seedless grapes, but it should be noted that when the concentration is 1000 ppm, yellowing, drying, and leaf shedding of leaves are likely to occur.

[0004] Another key factor for exogenous application of 1-aminocyclopropane-1-carboxylic acid to stimulate endogenous ethylene synthesis is ACC oxidase, whose activity is extremely unstable and depends on the integrity of the membrane. At the same time, when applying ACC alone, there may be the same application risks as ethephon preparations: it is likely to cause negative phenomena such as leaf shedding, fruit cracking, fruit drop, and variety decline in plants. Summary of the Invention

[0005] The objective of the present invention is to provide a fruit coloring composition that can effectively reduce the dosage of ACC used for coloring, improve the safety of exogenous application of ACC, and effectively improve the fruit planting effect, such as preventing fruit drop, increasing the fruit sweetness, reducing phytotoxicity, and relieving the growth inhibition of stems, leaves, and fruits.

[0006] The second objective of the present invention is to provide a fruit coloring functional fertilizer to solve the above problems.

[0007] The third objective of the present invention is to provide the applications of the above fruit coloring composition and the fruit coloring functional fertilizer in improving the fruit planting effect to solve the problems of efficient, safe, and green use of ACC.

[0008] To achieve the above object, the technical solution adopted by the present invention is as follows:

[0009] A fruit coloring composition, the fruit coloring composition comprising 1-aminocyclopropanecarboxylic acid and a protective auxiliary agent, and the mass ratio of 1-aminocyclopropanecarboxylic acid to the protective auxiliary agent is 1:0.1 to 200; the protective auxiliary agent is selected from 5-aminolevulinic acid, diethyl aminoethyl hexanoate or choline chloride.

[0010] The present invention first discloses the use of protective auxiliary agents such as 5-aminolevulinic acid, which can effectively improve the adverse effects when ACC is applied alone, such as high coloring dosage (300-500 ppm), which is likely to cause negative phenomena such as plant defoliation, fruit cracking, fruit drop and quality decline. When the effective application dosage is only 0.05-2.5 ppm, excellent coloring effect can be achieved. At the same time, the safety of exogenous application of ACC is effectively improved, and the planting effect of fruit and vegetable crops can be improved in terms of preventing fruit drop, increasing fruit sweetness, reducing phytotoxicity, and relieving the growth inhibition of stems, leaves and fruits.

[0011] To further optimize the use effect of the fruit coloring composition in aspects such as improving coloring and preventing fruit drop, preferably, the mass ratio of 1-aminocyclopropanecarboxylic acid to 5-aminolevulinic acid is 1:0.1 to 10; the mass ratio of 1-aminocyclopropanecarboxylic acid to diethyl aminoethyl hexanoate is 1:1 to 50; the mass ratio of 1-aminocyclopropanecarboxylic acid to choline chloride is 1:10 to 200.

[0012] A fruit coloring functional fertilizer, comprising the above fruit coloring composition, a water-soluble fertilizer raw material and an organic matter raw material, and the solvent is water.

[0013] The fruit coloring functional fertilizer provided by the present invention makes the above fruit coloring composition into a liquid fertilizer form, and the fertilizer and medicine are applied at one time, which is convenient to use and reduces the planting burden of fruit and vegetable crops.

[0014] To further improve the effects of the above fruit coloring composition in aspects such as improving coloring and preventing fruit drop, preferably, in the fruit coloring functional fertilizer, the mass fraction of the fruit coloring composition calculated based on the mass fraction of 1-aminocyclopropanecarboxylic acid is 0.1-1%, the water-soluble fertilizer raw material is 5-10%, and the organic matter raw material is 1-15%.

[0015] To more safely and effectively exert the fertilizer efficiency of the fruit coloring functional fertilizer, preferably, the water-soluble fertilizer raw material is selected from at least one of potassium dihydrogen phosphate, boric acid, potassium sulfate, potassium pyrophosphate, dipotassium hydrogen phosphate, tripotassium phosphate, potassium nitrate, potassium acetate, potassium lactate, potassium citrate. The organic matter raw material is selected from at least one of phenylalanine, methionine, proline, glutamic acid, lysine, alanine, citric acid, lactic acid, succinic acid, fructic acid, glucose, sucrose, trehalose, molasses liquid, sugar alcohol.

[0016] The applications of the above fruit coloring composition and fruit coloring functional fertilizer in improving the effects of fruit cultivation, said applications include promoting fruit coloring, and also include at least one of preventing fruit drop, increasing fruit sweetness, reducing phytotoxicity, and relieving the inhibition of stem, leaf and fruit growth.

[0017] Using the above fruit coloring composition and fruit coloring functional fertilizer can effectively improve the deficiency of exogenous application of ACC, and achieve the efficient, safe, green and scientific use of ACC.

[0018] Preferably, the fruit is apple, grape or strawberry; wherein, the application concentration of 1-aminocyclopropane carboxylic acid is 0.05 - 2.5 ppm. Applying the above fruit coloring composition and fruit coloring functional fertilizer to crops such as strawberries, apples, grapes, etc., after application, the growth of plant leaves and fruits is observed to be normal, no phytotoxicity symptoms are seen, and it can promote the fruit to color earlier, and at the same time improve the quality, and the soluble sugar content in the fruit increases.

[0019] Preferably, the application period is during the fruit color transition period, and the application method is drip irrigation or micro-sprinkler irrigation. Drip irrigation and micro-sprinkler irrigation are a fertilization or pesticide application method that uses plastic pipes to send water to the roots of plants through orifices or drippers for local irrigation or micro-sprinkling. Dilute and dissolve the liquid functional fertilizer in the irrigation water, and with the help of a pressure irrigation system, it is evenly and accurately transported to the soil at the roots of each plant by the drip irrigation pipe / tape, so as to realize the supply of water, fertilizer and pesticide to the crops for absorption and utilization in a precise combined state in the soil. The combination of the functional fertilizer and drip irrigation / micro-sprinkler irrigation realizes the integration of water and fertilizer, which can not only save water resources and labor, but also improve the utilization rate of pesticides and fertilizers, and promote farmers to save costs and increase income. Description of the Drawings

[0020] Figure 1 This is the comparison of the coloring effects of the blank control (CK), Example 3 (Treatment A3) and Comparative Example 1 (Treatment D1) on Fuji apples in Test Example 1 of the present invention;

[0021] Figure 2 This is the comparison of the coloring situations of different treatments (5 days after application) on Fuji apples in Test Example 1 of the present invention. From left to right, they are: blank control, Example 3, Comparative Example 1;

[0022] Figure 3 This is the comparison of the coloring effects of different treatments on Crimson Seedless grapes in Test Example 2 of the present invention;

[0023] Figure 4 This is the comparison of the coloring effects of different treatments on Hongyan strawberries in Test Example 3 of the present invention;

[0024] Figure 5 This is the influence of different treatments on the overall growth of strawberries in Test Example 3 of the present invention. From left to right, they are: Example 2, blank control, Comparative Example 1. Detailed implementation manners

[0025] In the prior art, the effective concentration range of ACC for promoting coloring is 300 - 500 ppm. When applied alone, it is likely to cause negative problems such as defoliation, fruit cracking, fruit drop, and quality decline of plants. The present invention aims to compound with ACC in the form of a protective adjuvant. On the one hand, it effectively reduces the effective concentration dose of ACC for promoting coloring and reduces its adverse effects. On the other hand, it improves the above problems existing in the application of ACC, improves the fruit quality, reduces phytotoxicity, and realizes the scientific and green use of ACC.

[0026] The present invention discovers that protective adjuvants such as 5-aminolevulinic acid, DCPTA, and choline chloride cooperate with ACC, which can achieve the above purposes. Even when the dosage of ACC is reduced by hundreds of times, it still exerts excellent coloring functions. At the same time, it effectively avoids the disadvantages such as defoliation, fruit cracking, fruit drop, and quality decline of plants caused by the single use of ACC. Verified on fruit and vegetable crops such as apples, strawberries, and grapes, its application effect is good.

[0027] Furthermore, the present invention recommends using a liquid functional fertilizer and applying it by irrigation or microspray. It has the conditions for automatic implementation, can save water resources and labor, and improve the utilization rate of raw materials. It is one of the main ways to realize large-scale planting and promote cost reduction and income increase for farmers.

[0028] During use, spraying the medicine 1 - 2 times by the above method during the fruit color-changing period can conveniently achieve the purpose of improving the coloring and quality of fruits. To make the technical objectives, technical solutions, and beneficial effects of the present invention clearer, the following specific embodiments are used to further illustrate the technical solutions of the present invention. However, the embodiments are intended to explain the present invention and should not be construed as a limitation of the present invention. For those without specific technologies or conditions noted in the embodiments, they shall be carried out according to the technologies or conditions described in the literature in this field or according to the product specifications.

[0029] I. Specific embodiments of the fruit coloring composition and fruit coloring functional fertilizer of the present invention

[0030] Example 1

[0031] The fruit coloring functional fertilizer of this example is composed of the following components by mass fraction: 0.1% 1-aminocyclopropane carboxylic acid, 1% 5-aminolevulinic acid, 5% potassium dihydrogen phosphate, 15% glucose, and the balance is water.

[0032] Among them, 1-aminocyclopropane carboxylic acid and 5-aminolevulinic acid form a fruit coloring composition, and the mass ratio of the two is 0.1:1.

[0033] The fruit coloring functional fertilizer of this embodiment is prepared according to the conventional production process of functional fertilizers: weigh the above raw materials according to the ratio, add them to a stirring kettle and stir and dissolve at room temperature. After passing the inspection, it is obtained.

[0034] Example 2

[0035] The fruit coloring functional fertilizer of this embodiment is composed of the following components by mass fraction: 1-aminocyclopropanecarboxylic acid 0.5%, 5-aminolevulinic acid 0.5%, potassium citrate 10%, citric acid 8%, and the balance is water.

[0036] Example 3

[0037] The fruit coloring functional fertilizer of this embodiment is composed of the following components by mass fraction: 1-aminocyclopropanecarboxylic acid 1%, 5-aminolevulinic acid 0.1%, potassium lactate 8%, fructonic acid 1%, and the balance is water.

[0038] Example 4

[0039] The fruit coloring functional fertilizer of this embodiment is composed of the following components by mass fraction: 1-aminocyclopropanecarboxylic acid 1%, choline chloride 10%, dipotassium hydrogen phosphate 10%, citric acid 1%, and the balance is water.

[0040] Example 5

[0041] The fruit coloring functional fertilizer of this embodiment is composed of the following components by mass fraction: 1-aminocyclopropanecarboxylic acid 0.5%, choline chloride 20%, potassium dihydrogen phosphate 6%, succinic acid 3%, and the balance is water.

[0042] Example 6

[0043] The fruit coloring functional fertilizer of this embodiment is composed of the following components by mass fraction: 1-aminocyclopropanecarboxylic acid 0.2%, choline chloride 40%, potassium lactate 5%, glucose 5%, and the balance is water.

[0044] Example 7

[0045] The fruit coloring functional fertilizer of this embodiment is composed of the following components by mass fraction: 1-aminocyclopropanecarboxylic acid 1%, diethyl aminoethyl hexanoate 1%, potassium lactate 5%, succinic acid 5%, and the balance is water.

[0046] Example 8

[0047] The fruit coloring functional fertilizer of this embodiment is composed of the following components by mass fraction: 1-aminocyclopropanecarboxylic acid 0.5%, diethyl aminoethyl hexanoate 6%, dipotassium hydrogen phosphate 10%, fructonic acid 3%, and the balance is water.

[0048] Example 9

[0049] The fruit coloring functional fertilizer of this embodiment is composed of the following components in mass fractions: 0.2% of 1-aminocyclopropanecarboxylic acid, 10% of yield-increasing amine, 6% of potassium citrate, 15% of glucose, and the balance is clean water.

[0050] The fruit coloring compositions of Examples 2 to 9 are determined accordingly with reference to the method of Example 1.

[0051] The fruit coloring functional fertilizers of Examples 2 to 9 were prepared by referring to the method of Example 1.

[0052] 2. Comparison

[0053] Comparative Example 1

[0054] Comparative Example 1: A functional fertilizer containing 1% 1-aminocyclopropanecarboxylic acid was prepared, which was composed of the following components in percentage by mass: 1% 1-aminocyclopropanecarboxylic acid, 8% potassium lactate, 1% fructose acid, and the balance was clean water.

[0055] Comparative Example 2

[0056] Comparative Example 2: A functional fertilizer containing 2% 5-aminolevulinic acid was prepared, which was composed of the following components in percentage by mass: 2% 5-aminolevulinic acid, 5% potassium dihydrogen phosphate, 15% glucose, and the balance being clean water.

[0057] Comparative Example 3

[0058] Comparative Example 3: A functional fertilizer containing 60% choline chloride was prepared, which was composed of the following components in percentage by mass: 60% choline chloride, 5% potassium lactate, 5% glucose, and the balance being clean water.

[0059] Comparative Example 4

[0060] Comparative Example 4: A functional fertilizer containing 8% of a yield-increasing amine was prepared, which was composed of the following components in percentage by mass: 8% of a yield-increasing amine, 6% of potassium citrate, 15% of glucose, and the balance being clean water.

[0061] 3. Application of fruit coloring composition and fruit coloring functional fertilizer

[0062] Test Example 1 Apple coloring, fruit drop and quality

[0063] Trial time: September-October 2021.

[0064] Experimental location: Yuliuqian Village, Qixia, Yantai City, Shandong Province.

[0065] Test agents: Examples 1-9 and control treatments (Comparative Examples 1-4). The specific test design of the test agents is shown in Table 1.

[0066] Table 1 Experimental design of the test agents for Fuji apple coloring test

[0067]

[0068]

[0069] Test crop: Fuji apples.

[0070] Application method: After removing the fruit bags of Fuji apples and at the initial stage of color change, drip irrigation was carried out once. Each treatment was diluted to the chemical concentration in Table 1 and then drip-irrigated (for example, the dilutions of Examples A1 - A3, B1 - B3, and C1 - C3 were 20,000 times before use), and the liquid volume was 10 L / plant.

[0071] Investigation method: On the day of application and 2, 5, and 19 days after application, the color grading of the fruits was investigated. Each time, the color grading of the marked fruits was counted, the color index was calculated, and at the same time, the number of fruit drops and the fruit drop rate were investigated; the occurrence of phytotoxicity was investigated 2 days after application; quality determination was carried out at harvest.

[0072] Among them, the color index was graded according to the percentage of the colored area on each fruit to the entire fruit area.

[0073] The grading standard was as follows:

[0074] Grade 0: Not colored;

[0075] Grade 1: The colored area accounted for less than 20% of the entire fruit area;

[0076] Grade 2: The colored area accounted for 21% - 40% of the entire fruit area;

[0077] Grade 3: The colored area accounted for 41% - 60% of the entire fruit area;

[0078] Grade 4: The colored area accounted for 61% - 80% of the entire fruit area;

[0079] Grade 5: The colored area accounted for 81% - 100% of the entire fruit area.

[0080]

[0081] The degree of phytotoxicity in each plot was distinguished by the phytotoxicity grading standard, represented by -, ++, +++, ++++.

[0082] -: No phytotoxicity;

[0083] +: Slight phytotoxicity, does not affect the normal growth of the crop;

[0084] ++: Obvious phytotoxicity, can recover, will not cause crop yield reduction;

[0085] +++: Severe phytotoxicity, affects the normal growth of the crop, causing a certain degree of loss to the crop yield and quality;

[0086] ++++: Severe phytotoxicity, crop growth is inhibited, and there are serious losses in yield and quality.

[0087] The evaluation method for soluble solid content is as follows: Use an intelligent digital refractometer to measure the total soluble solid content in the fruit, which can roughly represent the sugar content of the fruit. The higher the content, the higher the sugar degree and the better the quality.

[0088] Investigation results:

[0089] Compare the coloring situations of each treatment on the day of spraying and 2 days, 5 days, and 19 days after spraying. The coloring effects of the blank control (CK), Example 3 (Treatment A3), and Comparative Example 1 (Treatment D1) on Fuji are as Figure 1 shown. 5 days after spraying, the comparison of the coloring situations of the blank control, Example 3, and Comparative Example 1 is as Figure 2 shown. From left to right, they are: blank control, Example 3, and Comparative Example 1.

[0090] The overall data is shown in Table 2 below:

[0091] Table 2 Investigation results of coloring, fruit drop, and quality of Fuji apples 21 days after spraying

[0092]

[0093] It can be seen from the figures and data that applying the functional composition of the embodiments of the present invention can significantly promote the coloring of apples. Compared with the single-agent use, the coloring effect is remarkable, effectively inhibit fruit drop, the effect is stable, and it has a certain promotion on the quality of apples. Compared with the control agent and the blank treatment, the functional composition of the embodiments has an obvious improvement effect in promoting coloring, preventing fruit drop, and increasing the fruit sweetness.

[0094] Test Example 2 Coloring, cracking, and quality of grapes

[0095] Test time: June - July 2022.

[0096] Test location: Binchuan, Yunnan.

[0097] Test agents: Examples 1 - 9 and control treatments (Comparative Examples 1 - 4). The specific test agent test design is shown in Table 3.

[0098] Table 3 Test agent test design for coloring test of Crimson Seedless grapes

[0099] Treatment number Test agent Agent concentration A1 0.1% 1-aminocyclopropanecarboxylic acid + 1% 5-aminolevulinic acid (Example 1) 0.05 ppm + 0.5 ppm A2 0.5% 1-aminocyclopropanecarboxylic acid + 0.5% 5-aminolevulinic acid (Example 2) 0.25 ppm + 0.25 ppm A3 1% 1-aminocyclopropanecarboxylic acid + 0.1% 5-aminolevulinic acid (Example 3) 0.5 ppm + 0.05 ppm B1 1% 1-aminocyclopropanecarboxylic acid + 10% choline chloride (Example 4) 0.5 ppm + 5 ppm B2 0.5% 1-aminocyclopropanecarboxylic acid + 20% choline chloride (Example 5) 0.25 ppm + 10 ppm B3 0.2% 1-aminocyclopropanecarboxylic acid + 40% choline chloride (Example 6) 0.1 ppm + 20 ppm C1 1% 1-aminocyclopropanecarboxylic acid + 1% DCPTA (Example 7) 0.5 ppm + 0.5 ppm C2 0.5% 1-aminocyclopropanecarboxylic acid + 6% DCPTA (Example 8) 0.25 ppm + 3 ppm C3 0.2% 1-aminocyclopropanecarboxylic acid + 10% DCPTA (Example 9) 0.1 ppm + 5 ppm D1 1% 1-aminocyclopropanecarboxylic acid (Comparative Example 1) 5 ppm D2 2% 5-aminolevulinic acid (Comparative Example 2) 1 ppm D3 60% choline chloride (Comparative Example 3) 30 ppm D4 8% DCPTA (Example 4) 10 ppm CK Fresh water 0

[0100] Test crop: Crimson Seedless grapes.

[0101] Pesticide application method: Drip irrigation was carried out 2 times at the initial stage of color change, with an interval of 7 days. The water consumption for drip irrigation per mu was 5 tons, and 250 ml of functional fertilizer was used per mu, which was equivalent to diluting each treatment in the examples 20,000 times for drip irrigation. The D1 - D4 groups were diluted according to the agent concentrations in Table 3, and the water consumption for drip irrigation was the same as that in the examples.

[0102] Investigation method: On the day of pesticide application and on the 7th, 14th, 21st, and 30th days after application, the color grading of the fruits was investigated. Each time, the color grading of the marked fruits was counted, the color index was calculated, and the number of fallen fruits and the fruit drop rate were also investigated; The occurrence of phytotoxicity was investigated 3 days after application; The soluble solid content of the fruit grains was detected at harvest.

[0103] Among them, the color grading of grape fruits was evaluated according to the following criteria:

[0104] Grade 0: All green;

[0105] Grade 1: Slight coloring, with pink fruit grains accounting for 1% - 10% of the whole spike;

[0106] Grade 2: Colored fruit grains (pink) accounting for 10 - 50% of the fruit spike;

[0107] Grade 3: Colored fruit grains accounting for 50 - 100% of the whole spike;

[0108] Grade 4: All colored. Some black fruit grains appear, and black fruit grains account for 1 - 50% of the whole spike;

[0109] Grade 5: All colored, with more brown fruit grains, accounting for 50 - 100% of the whole spike.

[0110] The evaluation methods for the color index and soluble solid content were the same as those in Test Example 1.

[0111] Investigation results:

[0112] Comparing the coloring situations of each treatment 14 days after application, the representative treatment effects are as Figure 3 shown.

[0113] The overall data 30 days after application are as follows:

[0114] Table 4 Investigation results of the coloring, fruit drop and quality of Crimson Seedless grapes 30 days after pesticide application

[0115]

[0116] As can be seen from the figures and data, when treated with 1-aminocyclopropane carboxylic acid alone, the shattering phenomenon is serious, and the fruit drop rate reaches 90%. The functional composition of the embodiment of the present invention adding a protective auxiliary agent can significantly promote the coloring of grapes. Compared with the single agent, it has a faster coloring speed and obvious coloring effect, effectively inhibits fruit drop, has a stable effect, and has a certain promotion on the fruit quality. Compared with the control agent and the blank treatment, the functional composition of the embodiment has an obvious improvement effect in promoting coloring, preventing fruit drop and increasing the fruit sweetness.

[0117] Test Example 3 Strawberry Coloring Test Situation

[0118] Test time: December 2022.

[0119] Test location: Zhongmou, Henan.

[0120] Test agents: Examples 1-9 and control treatments (Comparative Examples 1-4). The specific test design of the test agents is shown in Table 5.

[0121] Table 5 Test Design of Test Agents for Strawberry Coloring Test

[0122] Treatment number Test agent Agent concentration A1 0.1% 1-aminocyclopropanecarboxylic acid + 1% 5-aminolevulinic acid (Example 1) 0.1 ppm + 1 ppm A2 0.5% 1-aminocyclopropanecarboxylic acid + 0.5% 5-aminolevulinic acid (Example 2) 0.5 ppm + 0.5 ppm A3 1% 1-aminocyclopropanecarboxylic acid + 0.1% 5-aminolevulinic acid (Example 3) 1 ppm + 0.1 ppm B1 1% 1-aminocyclopropanecarboxylic acid + 10% choline chloride (Example 4) 1 ppm + 10 ppm B2 0.5% 1-aminocyclopropanecarboxylic acid + 20% choline chloride (Example 5) 0.5 ppm + 20 ppm B3 0.2% 1-aminocyclopropanecarboxylic acid + 40% choline chloride (Example 6) 0.2 ppm + 40 ppm C1 1% 1-aminocyclopropanecarboxylic acid + 1% DCPTA (Example 7) 1 ppm + 1 ppm C2 0.5% 1-aminocyclopropanecarboxylic acid + 6% DCPTA (Example 8) 0.5 ppm + 6 ppm C3 0.2% 1-aminocyclopropanecarboxylic acid + 10% DCPTA (Example 9) 0.2 ppm + 10 ppm D1 1% 1-aminocyclopropanecarboxylic acid (Comparative Example 1) 5 ppm D2 2% 5-aminolevulinic acid (Comparative Example 2) 2 ppm D3 60% choline chloride (Comparative Example 3) 60 ppm D4 8% DCPTA (Example 4) 10 ppm CK Fresh water 0

[0123] Test crop: Hongyan strawberry.

[0124] Application method: Drip irrigation treatment once at the initial color change stage. The drip irrigation water consumption per mu is 10 tons, and 1000 ml of functional fertilizer is used per mu, which is equivalent to diluting each treatment of the example 10000 times for drip irrigation. The D1-D4 groups were diluted according to the agent concentrations in Table 3.

[0125] Investigation method: On the day of application, fruits with the same coloring degree were selected, 20 fruits were fixed for each treatment, and the coloring situation of the fruits was investigated 3 and 5 days after application. Each time of investigation, the coloring grade of the marked fruits was counted and the full-red fruit rate was calculated. 7 days after application, whether there was phytotoxicity to strawberry flowers, leaves and fruits was investigated; at harvest, the soluble solid content of the fruit grains was detected.

[0126]

[0127] The investigation and evaluation methods of phytotoxicity level and soluble solid content are the same as those in Test Example 1.

[0128] Investigation results:

[0129] Comparing the coloring situations of each treatment 3 and 5 days after application, the representative treatment effects are as Figure 4 shown.

[0130] 7 days after treatment, the effects of each treatment on the overall growth of strawberries were investigated. Except for Comparative Example 1, the growth of other treatments was normal. The treatment with Comparative Example 1 (1-aminocyclopropane carboxylic acid) inhibited the growth of strawberry stems, leaves and fruits, and side effects such as shortened stem nodes, red and brown edges of leaves, and deformed fruits appeared. The specific manifestations are shown in Figure 5 , from left to right are: Example 2, blank control, Comparative Example 1.

[0131] The results of the investigation on coloring and quality 5 days after treatment are as follows:

[0132] Table 6 Investigation results of coloring, fruit drop and quality of strawberries 5 days after treatment

[0133]

[0134] It can be seen from the figures and data that the treatment with 1-aminocyclopropane carboxylic acid alone seriously affects the growth of strawberries, causes a reduction in production in actual production, and results in economic losses. The functional composition of the embodiment of the present invention with a protective additive has an obvious promoting effect on promoting coloring and improving fruit quality compared with the control agent and the blank treatment.

Claims

1. A fruit coloring composition, characterized in that, The fruit coloring composition consists of 1-aminocyclopropane carboxylic acid and a protective adjuvant, and the mass ratio of 1-aminocyclopropane carboxylic acid to the protective adjuvant is 1:0.1 - 200; the protective adjuvant is selected from 5-aminolevulinic acid, DCPTA or choline chloride.

2. The fruit coloring composition according to claim 1, wherein The mass ratio of 1-aminocyclopropane carboxylic acid to 5-aminolevulinic acid is 1:0.1 - 10; the mass ratio of 1-aminocyclopropane carboxylic acid to DCPTA is 1:1 - 50; the mass ratio of 1-aminocyclopropane carboxylic acid to choline chloride is 1:10 - 200.

3. A fruit coloring functional fertilizer, characterized in that, It includes the fruit coloring composition according to claim 1 or 2, a water-soluble fertilizer raw material and an organic matter raw material, and the solvent is water.

4. The fruit coloring functional fertilizer according to claim 3, wherein In the fruit coloring functional fertilizer, the mass fraction of the fruit coloring composition calculated based on the mass fraction of 1-aminocyclopropane carboxylic acid is 0.1 - 1%, the water-soluble fertilizer raw material is 5 - 10%, and the organic matter raw material is 1 - 15%.

5. The fruit coloring functional fertilizer according to claim 3 or 4, characterized in that The water-soluble fertilizer raw material is selected from at least one of potassium dihydrogen phosphate, boric acid, potassium sulfate, potassium pyrophosphate, dipotassium hydrogen phosphate, tripotassium phosphate, potassium nitrate, potassium acetate, potassium lactate, potassium citrate.

6. The fruit coloring functional fertilizer according to claim 3 or 4, characterized in that The organic matter raw material is selected from at least one of phenylalanine, methionine, proline, glutamic acid, lysine, alanine, citric acid, lactic acid, succinic acid, fructic acid, glucose, sucrose, trehalose, molasses solution, sugar alcohol.

7. Use of the fruit coloring composition according to claim 1 or 2, and the fruit coloring functional fertilizer according to any one of claims 3 to 6, in improving the fruit planting effect, characterized in that, The application includes promoting fruit coloring, and also includes at least one of preventing fruit drop, increasing fruit sweetness, reducing phytotoxicity, and relieving the growth inhibition of stems, leaves and fruits.

8. The application according to claim 7, characterized in that, The fruit is an apple, a grape or a strawberry; among them, the application concentration of 1-aminocyclopropane carboxylic acid is 0.05 - 2.5 ppm.

9. The application according to claim 7 or 8, characterized in that, The using period is during the fruit color-changing period, and the using method is drip irrigation or micro-sprinkler irrigation.

Images