A skeletal muscle contraction response test device

Abstract

The application relates to a skeletal muscle contraction response test device, and belongs to the technical field of medical instruments, which solves the problem that the placement position of a tension sensor is different each time test operation is performed, the tension of a pulling line cannot be finely adjusted, and test failure is easily caused in the prior art. The skeletal muscle contraction response test device comprises a reaction box, a pulling line and a measuring assembly, and the pulling line is connected with the reaction box and the measuring assembly respectively. The tensioner of the application can make the tension unit away from or close to the water bath assembly, increase or reduce the supporting force on the pulling line, further tighten or loosen the pulling line, finely adjust the tension of the pulling line, and ensure the test effect.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of medical devices, in particular to a skeletal muscle contraction response test device. BACKGROUND

[0002] Malignant hyperthermia is a rare genetic disease that can be induced by inhalation of general anesthetics and depolarizing muscle relaxants succinylcholine, which is difficult to diagnose and treat, and is the most serious complication during the perioperative period. If it cannot be quickly diagnosed and treated after it occurs, the patient's condition will develop rapidly, and the mortality rate is extremely high.

[0003] Internationally, the caffeine-fluothane skeletal muscle contraction test has been proposed as the standard diagnostic method for malignant hyperthermia. The specific operation method is as follows: take 2-3 cm of fresh quadriceps femoris muscle or other long muscle near the tendon part of the skeletal muscle, fix it in 37℃ constant temperature Krebs solution (bicarbonate buffer solution) and continuously input 5% (volume ratio) carbon dioxide containing oxygen (hereinafter referred to as oxygen), and measure the change of muscle tension under the action of different concentrations of caffeine and / or fluothane. As the gold standard for diagnosing malignant hyperthermia, this test scheme is difficult to screen in susceptible individuals without known pathogenic gene mutations due to its high detection cost.

[0004] The tension sensor of the detection equipment at home and abroad is separately arranged. When the pulling line for pulling the test skeletal muscle is connected with the tension sensor, the test operator needs to set the placement position of the tension sensor to tension the pulling line. The placement position of the tension sensor is different each time, and the tension of the pulling line cannot be finely adjusted. Therefore, there is an urgent need for a skeletal muscle contraction response test device to solve the above problems. SUMMARY

[0005] In view of the above analysis, the embodiments of the present application aim to provide a skeletal muscle contraction response test device to solve the problem that the placement position of the tension sensor is different each time in the prior art, and the tension of the pulling line cannot be finely adjusted, which easily causes test failure.

[0006] The purpose of the present application is mainly realized through the following technical solutions:

[0007] A skeletal muscle contraction response test device, comprising a reaction box, a pulling line and a measuring assembly, the pulling line being connected with the reaction box and the measuring assembly respectively.

[0008] Further, the reaction box comprises a box body, and the box body is a hollow structure.

[0009] Further, one end of the pulling line is provided with a first hook, and the inner wall of one end of the box body is provided with a second hook.

[0010] Further, the box body is provided with a liquid inlet and an air inlet.

[0011] Further, the water bath assembly is a hollow structure, and the water bath assembly ensures that the reaction box is kept at a constant temperature.

[0012] Further, the water bath assembly comprises a box body and a partition plate, and the partition plate is arranged on the box body, and the box body and the partition plate jointly form a hollow structure.

[0013] Further, the water bath assembly further comprises a partition compartment, and the partition compartment is arranged on the partition plate, and the partition compartment is a cylindrical structure, one end of the partition compartment is an open end, and the other end of the partition compartment is a bottom end, and the reaction box can be inserted into the partition compartment from the open end.

[0014] Further, the bottom end of the partition compartment is provided with a liquid inlet pipe and an air inlet pipe, the liquid inlet pipe can be connected with the liquid inlet, and the air inlet pipe can be connected with the air inlet.

[0015] Further, the measuring assembly comprises a tension sensor, and the tension sensor is arranged on the side wall of the box body.

[0016] Further, the tension sensor comprises a measuring rod, and one end of the traction line is connected with the measuring rod.

[0017] Compared with the prior art, the present application can at least realize one of the following beneficial effects:

[0018] (1) The reaction box of the present application can be inserted into the partition compartment, and the partition compartment prevents the water bath liquid from contacting the reaction box, and provides waterproof protection for the reaction box when the water bath is heated;

[0019] (2) The hollow structure of the box body of the present application is provided with a heater, and the heater can keep the water bath liquid at a constant temperature of 37 DEG C, and provide a constant temperature water bath environment of 37 DEG C for the reaction box;

[0020] (3) The screw of the present application can drive the nut and the tensioning unit away from or close to the water bath assembly, increase or decrease the supporting force of the traction line, so as to further tighten or relax the traction line, and fine tune the tensioning force of the traction line, and ensure the test effect;

[0021] (4) The baffle of the present application buffers the flurane gas and oxygen entering the box body from the air inlet, avoids the gas directly blowing to the test skeletal muscle, reduces the shock to the test skeletal muscle, and ensures that the test skeletal muscle is always soaked in the Krebs solution;

[0022] (5) The harmless treatment assembly of the present application can shield the reaction box during the skeletal muscle contraction test, prevent the flurane gas from overflowing, and ensure the safety of the test personnel. BRIEF DESCRIPTION OF DRAWINGS

[0023] The accompanying drawings are only for the purpose of illustrating specific embodiments and are not considered to be a limitation on the present application, and in the whole drawings, the same reference signs represent the same parts.

[0024] Figure 1It is the overall structure schematic diagram of the experimental device of the present application;

[0025] Figure 2 It is the overall structure schematic diagram of the reaction box;

[0026] Figure 3 It is the longitudinal section structure schematic diagram of the reaction box;

[0027] Figure 4 It is the overall structure schematic diagram of the tensioner;

[0028] Figure 5 It is the internal structure schematic diagram of the experimental device of the present application.

[0029] Reference signs:

[0030] 1-reaction box; 2-pulling line; 3-water bath assembly; 4-tensioner; 5-measuring assembly; 6-innoculation treatment assembly; 11-box body; 12-turning part; 13-first electrode; 14-second electrode; 15-liquid inlet; 16-gas inlet; 21-first hook; 22-second hook; 23-baffle; 31-box body; 32-baffle; 33-compartment; 34-third electrode; 35-fourth electrode; 36-liquid inlet pipe; 37-gas inlet pipe; 38-heater; 51-tension sensor; 52-protrusion; 61-vent; 62-fan; 63-filter layer; 100-tested skeletal muscle; 121-supporting rod; 122-supporting base; 123-clamping seat. DETAILED DESCRIPTION

[0031] The preferred embodiments of the present application will be described in detail below with reference to the drawings, wherein the drawings constitute a part of the present application and serve to explain the principles of the embodiments of the present application, but are not used to limit the scope of the present application.

[0032] One specific embodiment of the present application, as shown in Figure 1 discloses a skeletal muscle contraction reaction experimental device (hereinafter referred to as experimental device), which comprises a reaction box 1, a pulling line 2, a water bath assembly 3, a tensioner 4, a measuring assembly 5 and an innoculation treatment assembly 6, the reaction box 1, the tensioner 4, the measuring assembly 5 and the innoculation treatment assembly 6 are all arranged on the water bath assembly 3, and the pulling line 2 is connected with the reaction box 1, the tensioner 4 and the measuring assembly 5 respectively. The reaction box 1 is used for containing a tested skeletal muscle 100 and Krebs solution, the reaction box 1 is connected with a fluorine alkane gas, and the measuring assembly 5 is used for detecting the contraction condition of the tested skeletal muscle 100. The experimental device of the present application is suitable for a caffeine-fluorine alkane skeletal muscle contraction test.

[0033] Preferably, as shown in Figure 2As shown, the reaction box 1 comprises a box body 11 and a crossbar 12. The box body 11 is a hollow structure, and one end of the box body 11 is open to put the pulling wire 2 and the subject skeletal muscle 100. The crossbar 12 is arranged at one end of the box body 11, the pulling wire 2 is overlapped on the crossbar 12 and changes the pulling direction, one end of the pulling wire 2 is connected with the measuring assembly 5 to record the muscle pulling force value, and the crossbar 12 and the measuring assembly 5 jointly ensure that the pulling wire 2 is in a taut state.

[0034] Preferably, as Figure 3 As shown, the other end of the pulling wire 2 is provided with a first hook 21, and the inner wall of one end of the box body 11 is provided with a second hook 22, and the two ends of the subject skeletal muscle 100 are connected with the first hook 21 and the second hook 22 respectively. The pulling wire 2 pulls the subject skeletal muscle 100 through the first hook 21, so that the subject skeletal muscle 100 is kept in a taut state.

[0035] Preferably, the side wall of the box body 11 is provided with a first electrode 13 and a second electrode 14. The first electrode 13 is connected with the first hook 21 through a wire, and the other end is connected with a third electrode 34, and the second electrode 14 is connected with the second hook 22 through a wire, and the other end is connected with a fourth electrode 35. An electric stimulation device (not shown in the figure) can be connected with the first electrode 13 and the second electrode 14 to electrify the subject skeletal muscle 100, and the subject skeletal muscle 100 is electrically stimulated to form muscle contraction.

[0036] The electrodes of the electric stimulation device in the prior art are arranged independently of the test device, and when used, the electrodes of the electric stimulation device need to be inserted into the reaction box 1, and the position of the electrodes of the electric stimulation device needs to be adjusted according to the different positions of the subject skeletal muscle 100 set for each test, so that the test operation is complicated and the consistency of the test operation is poor. The first electrode 13 and the second electrode 14 are arranged on the box body 11 of the test device, so that the electrodes of the electric stimulation device are no longer arranged independently of the reaction box 1, and the electrodes of the electric stimulation device do not need to be specially connected in the box body 11 during the test operation, which saves the test time and facilitates the test operation.

[0037] Preferably, the crossbar 12 is a movable structure, which comprises a support rod 121 and a support base 122, the support base 122 is arranged on the inner wall of the box body 11, one end of the support rod 121 is hinged to the support base 122, and the support rod 121 can rotate around the support base 122 to open the box body 11. The inner wall of the box body 11 is also provided with a clamping seat 123, and the other end of the support rod 121 can be clamped into the clamping seat 123, so that the support rod 121 cannot produce radial displacement. When the support rod 121 is opened, the subject skeletal muscle 100 is conveniently put into the box body 11, and after the support rod 121 is clamped into the clamping seat 123, the crossbar 12 remains stable, and the taut state of the pulling wire 2 is also kept stable at all times.

[0038] Preferably, the bottom end of the box body 11 is also provided with a liquid inlet 15 and an air inlet 16. The liquid inlet 15 is connectable with a liquid inlet pipe 36 for conveying the coffee liquid into the box body 11. The air inlet 16 is connectable with an air inlet pipe 37 for conveying the oxygen and the halothane gas into the box body 11.

[0039] Preferably, the second hook 22 is further provided with a baffle 23 arranged at the opening of the air inlet 16. The baffle 23 is a flat plate, and the surface of the flat plate is perpendicular to the direction of the air flow. The baffle 23 buffers the halothane gas and the oxygen entering the box body 11 through the air inlet 16, avoids the gas directly blowing to the subject skeletal muscle 100, reduces the shock to the subject skeletal muscle 100, and ensures that the subject skeletal muscle 100 is always soaked in the Krebs solution.

[0040] Preferably, as shown in Figure 4 The tensioner 4 includes an adjusting piece 41, a tensioning unit 42, a nut 43, and a screw rod 44. By adjusting the tensioner 4, the tension of the pull wire 2 can be adjusted.

[0041] Preferably, one end of the adjusting piece 41 is fixedly connected with the water bath assembly 3, and the other end of the adjusting piece 41 is a movable end. The movable end can be close to or away from the water bath assembly 3.

[0042] Preferably, the tensioning unit 42 is connected with the movable end of the adjusting piece 41. The pull wire 2 can be overlapped on the tensioning unit 42, and the pull wire 2 can slide on the tensioning unit 42 and be supported and converted in the pulling direction by the tensioning unit 42, and finally connected with the measuring assembly 5. The tensioning unit 42 can increase or decrease the supporting force of the pull wire 2 by moving away from or close to the water bath assembly 3 with the movable end of the adjusting piece 41, so as to further tighten or relax the pull wire 2.

[0043] Preferably, the tensioning unit 42 can be a tensioning rod. One end of the tensioning rod is connected with the movable end of the adjusting piece 41, and the surface of the tensioning rod is a smooth arc surface, so as to ensure that the sliding friction resistance of the pull wire 2 is minimized.

[0044] Alternatively, the tensioning unit 42 can also be a tensioning roller. The tensioning roller is rotatably connected with the movable end of the adjusting piece 41, and an annular groove is arranged on the rolling surface. The pull wire 2 can be arranged in the annular groove, so as to ensure that the resistance of the pull wire 2 is minimized while ensuring that the pull wire 2 can always be aligned with the measuring assembly 5.

[0045] Preferably, the nut 43 is arranged on the adjusting piece 41, the nut 43 and the adjusting piece 41 are rotatably connected, and the screw rod 44 and the nut 43 are threadedly connected. The adjusting piece 41 has elasticity, and the adjusting piece 41 can ensure that one end of the screw rod 44 is always connected with the outer wall of the water bath assembly 3.

[0046] The rotating screw 44 can drive the nut 43 and the tensioning unit 42 away from or close to the water bath assembly 3, thereby increasing or reducing the supporting force on the traction line 2, so as to further tighten or relax the traction line 2.

[0047] Preferably, as shown in Figure 5 The water bath assembly 3 is a hollow structure, which can contain 37°C constant temperature water bath liquid, so as to ensure that the reaction box 1 is kept at a constant temperature of 37°C.

[0048] Preferably, the water bath assembly 3 comprises a box body 31 and a partition plate 32, the partition plate 32 is arranged on the box body 31, and the box body 31 and the partition plate 32 jointly form the hollow structure.

[0049] Preferably, the water bath assembly 3 further comprises a partition compartment 33, the partition compartment 33 is arranged on the partition plate 32, and the partition compartment 33 is a cylindrical structure with an open end and a bottom end. The reaction box 1 can be inserted into the partition compartment 33 from the open end. The partition compartment 33 prevents the water bath liquid from contacting the reaction box 1, and provides waterproof protection for the reaction box 1 when the water bath is heated.

[0050] Preferably, the third electrode 34 and the fourth electrode 35 are arranged on the side wall of the partition compartment 33. After the reaction box 1 is inserted into the partition compartment 33, the first electrode 13 can be connected with the third electrode 34, and the second electrode 14 can be connected with the fourth electrode 35. The third electrode 34 and the fourth electrode 35 are also connected with two poles of an electric stimulation device. The electric stimulation device can supply electricity to the reaction box 1, so as to finally stimulate the subject skeletal muscle 100 to form muscle contraction.

[0051] Preferably, the bottom end of the partition compartment 33 is further provided with a liquid inlet pipe 36 and an air inlet pipe 37. One end of the liquid inlet pipe 36 is connected with a drug supply device (not shown in the figure), and the other end can be connected with the liquid inlet 15, so as to transport caffeine liquid into the reaction box 1 through the partition compartment 33. One end of the air inlet pipe 37 is connected with an air supply device (not shown in the figure), and the other end can be connected with the air inlet 16, so as to transport oxygen and halothane gas into the reaction box 1 through the partition compartment 33.

[0052] Preferably, the water bath assembly 3 further comprises a heater 38, which is arranged on the inner wall of the box body 31. The heater 38 comprises an electric heating wire and a temperature sensor (not shown in the figure), and can keep the water bath liquid at a constant temperature of 37°C.

[0053] Preferably, as shown in Figure 1 The measurement assembly 5 comprises a tension sensor 51, which is arranged on the side wall of the box body 31. The tension sensor 51 comprises a measuring rod. The traction line 2 can be overlapped on the cross rod 12 and the tensioner 4, and finally one end of the traction line 2 is connected with the measuring rod of the tension sensor 51.

[0054] In view of the high precision of the tension sensor 51, the tension sensor 51 needs to be stored separately, so the box body 31 is also provided with a protrusion 52, and the side wall of the tension sensor 51 is provided with a recess (not shown in the figure), which can be clamped with the protrusion 52. When the tension sensor 51 is not needed, the tension sensor 51 can be separated from the box body 31 and stored separately.

[0055] Preferably, as shown in the figure, the harmless treatment assembly 6 is movably connected with the water bath assembly 3, and the harmless treatment assembly 6 can shield the reaction box 1 during the skeletal muscle contraction test, preventing the fluorine gas from overflowing, and ensuring the safety of the test personnel. Figure 5

[0056] Preferably, the harmless treatment assembly 6 includes a ventilation port 61, a fan 62 and a filter layer 63, the ventilation port 61 is arranged on the side wall of the harmless treatment assembly 6 facing the water bath assembly 3, and the fan 62 and the filter layer 63 are arranged in the harmless treatment assembly 6. The fan 62 rotates to suck the fluorine gas in the reaction box 1 into the harmless treatment assembly 6, and the fluorine gas is filtered by the filter layer 63 and adsorbed by the filter layer 63, preventing the fluorine gas from overflowing the test device and being inhaled by the test personnel, and ensuring the safety of the test personnel.

[0057] Preferably, the filter layer 63 is provided with a neutralizing liquid, which can neutralize the fluorine gas, and the fluorine gas is combined or decomposed into substances without anesthetic effect, further ensuring the safety of the test personnel.

[0058] In use in surgery, water bath liquid is added to the box body 31 and the heater 38 is turned on, so that the water bath assembly 3 can always maintain a constant temperature of 37℃; the support rod 121 is rotated, the box body 11 is opened, the test skeletal muscle 100 is hung by the first hook 21 and the second hook 22, the support rod 121 is rotated, the box body 11 is closed, the pull wire 2 is lapped on the support rod 121, krebs liquid is poured into the reaction box 1, then the reaction box 2 is inserted into the partition 33, and the insertion can complete the connection of the first electrode 13 and the third electrode 34 and the connection of the second electrode 14 and the fourth electrode 35, and at the same time, the liquid inlet pipe 36 is inserted into the liquid inlet 15, and the gas inlet pipe 37 is inserted into the gas inlet 16; oxygen is introduced into the reaction box 1; the pull wire 2 is lapped on the tensioning unit 42 of the tensioner 4, and the pull wire 2 is tightened; one end of the pull wire 2 is connected with the tension sensor 51, the screw rod 44 is rotated, the position of the tensioning unit 42 is adjusted, the supporting force of the pull wire 2 is changed, and the pre-tightening force of the pull wire 2 is adjusted to 0.2 Newton through the reading of the tension sensor 51 at this time; the test skeletal muscle 100 is electrically stimulated through the first electrode 13 and the second electrode 14, and the reading is recorded by the tension sensor 51 as a control group; the caffeine liquid and / or the fluorine gas are introduced into the reaction box 1 through the liquid inlet pipe 36 and the gas inlet pipe 37 to perform the skeletal muscle contraction test.

[0059] ​The test device of the embodiment can sample in surgery and immediately perform the test in the test device without sending to a special test room, reducing the test time and facilitating the rapid surgery; the rotating screw rod 44 can drive the nut 43 and the tensioning unit 42 to move away from or close to the water bath assembly 3, increasing or reducing the supporting force on the traction line 2, so as to further tighten or relax the traction line 2; the first electrode 13 and the second electrode 14 are integrated on the box body 11, so that the electrodes of the electric stimulation device are no longer arranged independently of the reaction box 1, and the test operation does not need to specially connect the electrodes of the electric stimulation device in the box body 11, saving the test time and facilitating the test operation; the baffle 23 buffers the fluorane gas and oxygen entering the box body 1 from the air inlet 16, avoiding the gas directly blowing to the test skeletal muscle 100, and not causing concussion to the test skeletal muscle 100, ensuring that the test skeletal muscle 100 is always soaked in the Krebs solution; the reaction box 1 can be inserted into the partition compartment 33 from the open end, the partition compartment 33 prevents the water bath liquid from contacting the reaction box 1, and provides waterproof protection for the reaction box 1 when the water bath is heated; the harmless treatment assembly 6 can shield the reaction box 1 during the skeletal muscle contraction test, prevent the fluorane gas from overflowing, and ensure the safety of the test personnel.

[0060] The above is only the preferred specific embodiment of the present application, but the protection scope of the present application is not limited to this, any person skilled in the art can easily think of changes or replacements within the technical range disclosed by the present application, which should be covered in the protection scope of the present application.

Claims

1. A skeletal muscle contractile response test device, characterized by, The test device is used for caffeine-fluothane skeletal muscle contraction test, and comprises a reaction box (1), a pull wire (2) and a measuring assembly (5), wherein the pull wire (2) is used for connecting the reaction box (1) and the measuring assembly (5). The reaction box (1) comprises a box body (11), a crossbar (12), a liquid inlet (15) and an air inlet (16), the box body (11) is a hollow structure, one end of the box body (11) is open, and the pull wire (2) and a test skeletal muscle (100) are arranged in the box body (11); the crossbar (12) is arranged at one end of the box body (11), the pull wire (2) is overlapped on the crossbar (12) and changes the pulling direction, one end of the pull wire (2) is connected with the measuring assembly (5), and the muscle pulling force value is recorded. The crossbar (12) comprises a support rod (121) and a support base (122), the support base (122) is arranged on the inner wall of the box body (11), and one end of the support rod (121) is hinged to the support base (122); the inner wall of the box body (11) is further provided with a clamping seat (123), the other end of the support rod (121) can be clamped into the clamping seat (123), and the clamping seat (123) makes the support rod (121) not displace in the radial direction. One end of the pull wire (2) is provided with a first hook (21), and the inner wall of one end of the box body (11) is provided with a second hook (22); the liquid inlet (15) and the air inlet (16) are arranged at the bottom end of the box body (11), the second hook (22) is provided with a baffle (23), and the baffle (23) is arranged at the opening of the air inlet (16); the surface of the baffle (23) is perpendicular to the airflow direction; the baffle (23) buffers the fluothane gas and oxygen entering the box body (11) from the air inlet (16), so that the gas is prevented from directly blowing to the test skeletal muscle (100).

2. The skeletal muscle contractile response test device of claim 1, wherein, The water bath assembly (3) is further provided with a water bath assembly (3), and the water bath assembly (3) is a hollow structure.

3. The skeletal muscle contractile response test device of claim 2, wherein, The water bath assembly (3) comprises a box body (31) and a partition plate (32), and the partition plate (32) is arranged on the box body (31).

4. The skeletal muscle contractile response test device of claim 3, wherein, The water bath assembly (3) further comprises a partition compartment (33), and the partition compartment (33) is arranged on the partition plate (32) and is a cylindrical structure, one end of the partition compartment (33) is an open end, and the other end is a bottom end, and the reaction box (1) can be inserted into the partition compartment (33) from the open end.

5. The skeletal muscle contractile response test device of claim 4, wherein, The bottom end of the partition compartment (33) is provided with a liquid inlet pipe (36) and an air inlet pipe (37), the liquid inlet pipe (36) can be connected with the liquid inlet (15), and the air inlet pipe (37) can be connected with the air inlet (16).

6. The skeletal muscle contractile response test device of claim 3, wherein, The measuring assembly (5) comprises a tension sensor (51), and the tension sensor (51) is arranged on the side wall of the box body (31).

7. The skeletal muscle contractile response test device of claim 6, wherein, The tension sensor (51) comprises a measuring rod, and one end of the pull wire (2) is connected with the measuring rod.

Images