A Chinese medicine composition for treating hyperlipidemia and its preparation method and application

Abstract

The present invention provides a Chinese medicine composition for treating hyperlipidemia, and a preparation method and application thereof. The Chinese medicine composition, by weight, comprises: 4-12 parts of hawthorn, 8-12 parts of salvia miltiorrhiza, 4-12 parts of panax notoginseng and 4-12 parts of pearly buds. The preparation method comprises: (1) crushing the Chinese medicinal materials hawthorn, salvia miltiorrhiza, panax notoginseng and pearly buds to obtain a powder; (2) using an ethanol aqueous solution as an extraction solvent to extract the powder, and concentrating the obtained extract to obtain a compound extract of the Chinese medicine composition. Although pearly buds have no effect on lowering blood lipids, when it is used in combination with hawthorn, salvia miltiorrhiza and panax notoginseng, it can improve the blood lipid lowering effect of the hawthorn, salvia miltiorrhiza and panax notoginseng composition.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicines, and in particular relates to a traditional Chinese medicine composition for treating hyperlipidemia, and a preparation method and application thereof. Background Art

[0002] Hyperlipidemia has a high incidence rate worldwide, and its main characteristics are high levels of serum cholesterol (TC), serum triglycerides (TG), serum low-density lipoprotein (LDL-C) and low levels of high-density lipoprotein (HDL-C). Hyperlipidemia is also a high-risk factor for cardiovascular disease, obesity and other diseases. The prevention and treatment of hyperlipidemia mainly focus on reducing the levels of TC, TG, and LDL-C in serum and increasing the level of serum HLD-C, which can also reduce the incidence of chronic diseases.

[0003] For patients with hyperlipidemia, statins and fibrates are the first-line drugs of choice. However, the side effects of these drugs are also extremely obvious. The main side effects of statins are mania and liver function myopathy, and the main side effects of fibrates are increased serum creatinine levels, pancreatitis and sudden death.

[0004] Compared with Western medicine, Chinese medicine has the advantage of less side effects and obvious therapeutic effects in treating chronic diseases. Polygonum villosa is a traditional Tibetan medicine with the effects of clearing away heat and detoxifying, dispersing blood stasis and stopping bleeding. It is mainly used for enteritis, dysentery, etc. There is no research report on lowering blood lipids. Summary of the invention

[0005] In order to solve the above problems of the prior art, the present invention provides a Chinese medicine composition for treating hyperlipidemia and a preparation method and application thereof, which is used for treating hyperlipidemia and has significant therapeutic effect.

[0006] The present invention is achieved through the following technical solutions:

[0007] A traditional Chinese medicine composition for treating hyperlipidemia comprises, by weight, 4-12 parts of hawthorn, 8-12 parts of salvia miltiorrhiza, 4-12 parts of panax notoginseng and 4-12 parts of polygonum villosa.

[0008] Preferably, based on the weight percentage, it comprises: 9-12 parts of hawthorn, 10-12 parts of salvia miltiorrhiza, 9-12 parts of Panax notoginseng and 4-12 parts of Polygonum villosa.

[0009] Preferably, based on the weight percentage, it comprises: 9 parts of hawthorn, 10 parts of salvia miltiorrhiza, 9 parts of Panax notoginseng and 4 parts of Polygonum villosa.

[0010] Preferably, the composition comprises, by weight: 9 parts of hawthorn, 10 parts of salvia miltiorrhiza, 9 parts of panax notoginseng and 8 parts of polygonum villosa.

[0011] Preferably, based on the weight percentage, it comprises: 9 parts of hawthorn, 10 parts of salvia miltiorrhiza, 9 parts of Panax notoginseng and 12 parts of Polygonum villosa.

[0012] The method for preparing the Chinese medicine composition for treating hyperlipidemia comprises:

[0013] (1) crushing Chinese medicinal materials including hawthorn, salvia miltiorrhiza, notoginseng and Polygonum villosa to obtain medicinal powder;

[0014] (2) Using ethanol aqueous solution as an extraction solvent to extract the medicinal powder, and concentrating the obtained extract to obtain the compound extract of the traditional Chinese medicine composition.

[0015] Preferably, in step (1), the powder is sieved after being crushed to obtain a medicinal powder.

[0016] Preferably, step (2) is specifically as follows: first soaking the drug powder in an ethanol-water solution, then heating and refluxing for extraction, repeating the extraction several times, combining the filtrate, filtering to remove drug residues, recovering ethanol, concentrating and drying to obtain the compound extract.

[0017] The application of the traditional Chinese medicine composition in preparing medicine for treating hyperlipidemia.

[0018] A medicine for treating hyperlipidemia comprises the Chinese medicine composition and pharmaceutically acceptable excipients.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] The Chinese medicine composition of the present invention is composed of hawthorn, salvia miltiorrhiza, panax notoginseng, and pearl bud polygonum, which can reduce the levels of serum cholesterol, serum triglyceride, and serum low-density lipoprotein and improve the level of high-density lipoprotein; hawthorn can reduce the absorption of cholesterol; the water extract of salvia miltiorrhiza can also inhibit the oxidative modification of low-density lipoprotein, thereby reducing atherosclerosis; panax notoginseng can reduce the TC and TG levels of the liver and prevent the formation of fatty liver. Experimental results show that although pearl bud polygonum has no effect of lowering blood lipids, when it is used in combination with hawthorn, salvia miltiorrhiza, and panax notoginseng, it can improve the blood lipid lowering effect of the hawthorn, salvia miltiorrhiza, and panax notoginseng composition. The present invention can be made into conventional dosage forms such as tablets, granules, and capsules according to conventional preparation processes, and is used to treat hyperlipidemia, with significant efficacy, providing a new choice for the treatment of hyperlipidemia. DETAILED DESCRIPTION

[0021] In order to further understand the present invention, the present invention is described below in conjunction with embodiments. These descriptions are only to further explain the features and advantages of the present invention and are not intended to limit the claims of the present invention.

[0022] The invention discloses a Chinese medicine composition for treating hyperlipidemia, wherein the raw materials for its preparation include, by weight, 4-12 parts of hawthorn, 8-12 parts of salvia miltiorrhiza, 4-12 parts of notoginseng, and 4-12 parts of polygonum villosa, preferably 9-12 parts of hawthorn, 10-12 parts of salvia miltiorrhiza, 9-12 parts of notoginseng, and 4-12 parts of polygonum villosa.

[0023] The preparation method of the Chinese medicine composition for treating hyperlipidemia of the present invention comprises:

[0024] (1) Take Chinese medicinal materials including hawthorn, salvia miltiorrhiza, Panax notoginseng, and Polygonum villosa, grind them into powder, and sieve them; weigh the powders of the four medicinal materials according to the above proportions and mix them evenly;

[0025] (2) First, soak the drug powder in ethanol water solution, then heat and reflux for extraction, repeat the extraction several times, combine the filtrate, filter to remove the drug residue, recover the ethanol, concentrate and dry, and obtain the compound extract.

[0026] The raw materials and equipment used in the specific embodiments of the present invention are all known products and are obtained by purchasing commercially available products.

[0027] Example 1 Preparation of Chinese medicine composition

[0028] A. Weigh hawthorn, salvia miltiorrhiza, notoginseng and Polygonum villosa according to the components in the experimental groups and the control group in Table 1, crush them into fine powder, sieve them and mix them evenly.

[0029] B. Add 8 times the volume of ethanol solution (volume ratio is 75%) to the mixed medicinal material powder in step A, soak for 2 hours, heat reflux extraction for 1.5 hours, repeat the extraction twice, combine the extracts, concentrate under reduced pressure and dry to obtain extract powder.

[0030] Table 1 Composition of Chinese herbal medicine ingredients in the experimental group and the control group

[0031]

[0032]

[0033] Example 2: Reduction of four blood lipids in serum and cholesterol and triglyceride levels in liver in rats

[0034] A. Animal feeding and high-fat feeding

[0035] SD rats weighing 250-270g were selected. The normal group rats were allowed to eat normal feed and water freely, the model group rats were allowed to eat high-fat feed and water freely, and the drug-treated group rats were allowed to eat high-fat feed and water freely and were given the compound extract solution every day. The high-fat feed consisted of 65.55% basic feed, 20% lard, 10% sucrose, 4% cholesterol, 0.25% bile salt, and 0.2% methylthiouracil.

[0036] B. Experimental Methods

[0037] After a one-week adaptation period, 98 rats were divided into 14 groups, including blank group, model group, experimental group 1-8, and control group 1-4, with 7 rats in each group. The blank group and the model group were given water by gavage every day, and the experimental group 1-8 and the control group 1-4 were given the same dose of extract by gavage every day. The modeling and administration time was 28 days. The rats were fasted the day before the experiment and had free access to water. After anesthesia with 10% chloral hydrate, blood was collected from the abdominal aorta and the liver was taken. After the blood sample was left at room temperature for 2 hours, the serum was separated by centrifugation and stored at -80°C for testing.

[0038] C. Four tests of blood lipids in serum

[0039] Serum TC, TG, LDL-C, and HDL-C were detected and analyzed according to the kit instructions.

[0040] D. Detection of TC and TG in liver

[0041] 100 mg of liver tissue was cut from the same part of the rat and accurately weighed, and placed in a plastic centrifuge tube containing 0.2 m saline, and placed in an ice bath to prepare liver homogenate; then 0.8 ml of chloroform-methanol (1:1) extract was added to the liver homogenate, mixed thoroughly, and after standing for 18 hours, centrifuged at 3000 / pm for 10 minutes. The centrifuge tube was divided into three clear layers: upper, middle and lower. The upper phase was the aqueous phase, the middle phase was the broken tissue clumps, and the lower phase was the lipid layer; the lipids in the lower phase were carefully extracted and frozen at 4°C for testing.

[0042] E. Result Analysis

[0043] 1. The test results of TC, TG, LDL-C and HDL-C in serum are shown in Table 2. Compared with the blank group, the TC, TG and LDL-C in the model group were significantly increased, and the HDL-C was significantly decreased. After the intervention of the compound extract, the TC, TG and LDL-C in the experimental group 1-8 and the control group 1-2 were significantly decreased compared with the model group, and the HDL-C in the experimental group 1-8 and the control group 1-2 was significantly increased compared with the model group. The compound extract of the experimental group 1-8 was better than that of the control group 1-2, indicating that the compound extracts of the experimental group 1-8 and the control group 1-2 had the effect of lowering blood lipids. However, when the ratio of each raw material is not within the range set by the present invention, that is, when the amount of hawthorn and salvia miltiorrhiza added is too little, the blood lipid lowering effect decreases, and when the amount of Polygonum villosa added is too much, the blood lipid lowering effect will also decrease.

[0044] At the same time, it can be seen that the TC and LDL-C of the control group 3 were significantly lower than those of the model group, the TG of the control group 3 was not significantly changed compared with the model group, and the TC, TG, LDL-C and HDL-C of the control group 4 were not significantly changed compared with the model group, indicating that the composite extracts of hawthorn, salvia miltiorrhiza and Panax notoginseng in the control group 3 had a certain effect on lowering blood lipids, while the extracts of Polygonum villosa in the control group 4 had no effect on lowering blood lipids. Compared with the experimental group 1, the TC, TG and LDL-C of the control group 3 were higher than those of the experimental group 1, and the raw materials of the control group 3 were less than those of the experimental group 1, and the other raw materials and contents were the same, which showed that Polygonum villosa itself had no effect on lowering blood lipids, but when used in combination with hawthorn, salvia miltiorrhiza and Panax notoginseng, it could enhance the effect of the combination of hawthorn, salvia miltiorrhiza and Panax notoginseng on lowering blood lipids.

[0045] Table 2 Detection results of TC, TG, LDL-C and HDL-C in serum

[0046]

[0047]

[0048] 2. The results of TC and TG detection in the liver are shown in Table 3. As can be seen from Table 3, compared with the model group, after the intervention of the compound extract, the TC and TG in the liver of the experimental group 1-8 and the control group 1-2 were also significantly reduced, indicating that the compound extract can reduce the accumulation of TC and TG in the liver, but the TC and TG levels of the control group 1-2 were higher than those of the experimental group 1-8, indicating that when the amount of hawthorn and salvia miltiorrhiza added was too little, the lipid-lowering effect decreased, and when the amount of Polygonum villosa added was too much, the lipid-lowering effect would also decrease. The TC and TG in the liver of the control group 3 were also significantly reduced, but higher than the TC and TG in the liver of the experimental group 1. The TC and TG in the liver of the control group 4 were not significantly changed compared with the model group, indicating that Polygonum villosa itself did not have the effect of lowering blood lipids, but when used in combination with hawthorn, salvia miltiorrhiza, and Panax notoginseng, it could enhance the lipid-lowering effect of the combination of hawthorn, salvia miltiorrhiza, and Panax notoginseng.

[0049] Table 3 TC and TG detection results in liver

[0050]

[0051] The above contents are only for explaining the technical idea of ​​the present invention and cannot be used to limit the protection scope of the present invention. Any changes made on the basis of the technical solution in accordance with the technical idea proposed by the present invention shall fall within the protection scope of the claims of the present invention.

Claims

1. A Chinese medicine composition for treating hyperlipidemia, characterized in that: The preparation method is prepared from the following medicinal materials in parts by weight: 9-12 parts of hawthorn, 10-12 parts of salvia miltiorrhiza, 9-12 parts of panax notoginseng and 4-12 parts of polygonum villosa.

2. The Chinese medicine composition for treating hyperlipidemia according to claim 1, characterized in that: The preparation method is prepared from the following medicinal materials, according to the weight ratio: 9 parts of hawthorn, 10 parts of salvia miltiorrhiza, 9 parts of Panax notoginseng and 4 parts of Polygonum villosa.

3. The Chinese medicine composition for treating hyperlipidemia according to claim 1, characterized in that: The preparation method is prepared from the following medicinal materials, according to the weight ratio: 9 parts of hawthorn, 10 parts of salvia miltiorrhiza, 9 parts of Panax notoginseng and 8 parts of Polygonum villosa.

4. The Chinese medicine composition for treating hyperlipidemia according to claim 1, characterized in that: The preparation method is prepared from the following medicinal materials, according to the weight ratio: 9 parts of hawthorn, 10 parts of salvia miltiorrhiza, 9 parts of Panax notoginseng and 12 parts of Polygonum villosa.

5. The method for preparing the Chinese medicine composition for treating hyperlipidemia according to any one of claims 1 to 4, characterized in that: include: (1) crushing the Chinese medicinal materials hawthorn, salvia miltiorrhiza, Panax notoginseng and Polygonum villosa to obtain medicinal powder; (2) Using ethanol aqueous solution as an extraction solvent to extract the medicinal powder, and concentrating the obtained extract to obtain the compound extract of the traditional Chinese medicine composition.

6. The method for preparing the Chinese medicine composition for treating hyperlipidemia according to claim 5, characterized in that: In step (1), the powder is crushed and then sieved to obtain a medicinal powder.

7. The method for preparing the Chinese medicine composition for treating hyperlipidemia according to claim 5, characterized in that: Step (2) is specifically as follows: firstly soak the drug powder in ethanol-water solution, then heat and reflux for extraction, repeat the extraction several times, combine the filtrate, remove the drug residue by suction filtration, recover the ethanol, concentrate and dry, and obtain the compound extract.

8. Use of the Chinese medicine composition according to any one of claims 1 to 4 in the preparation of a drug for treating hyperlipidemia.

9. A drug for treating hyperlipidemia, characterized in that: The composition is prepared from the Chinese medicine composition according to any one of claims 1 to 4 and pharmaceutically acceptable excipients.

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