Fully automatic urine iodine analyzer and sample analysis method

Abstract

The present invention relates to a fully automatic urine iodine analyzer and a sample analysis method, which are characterized in that a mobile platform is arranged on an aluminum profile frame, an ultraviolet detector and a water-cooled heater are arranged in the three-dimensional moving space of the mobile platform, the mobile platform provides reagents to a digestion tube on the water-cooled heater through an injection needle, and the mobile platform also provides a test sample into the ultraviolet detector through the injection needle, and the ultraviolet detector detects the light intensity values ​​of the sample and a reference solution. The present invention integrates multiple functions of digestion, water bath, and detection, and the entire test process is highly automated, avoiding errors caused by human participation, and greatly improving the convenience and accuracy of the test.

Description

Technical Field

[0001] The invention relates to a full-automatic urine iodine analyzer and a sample analysis method. Background Art

[0002] Because urine iodine levels are a good indicator recommended by the WHO for assessing a population's iodine intake and nutritional status, and are widely used in national and provincial iodine nutritional monitoring, as well as in other related scientific research fields, urine iodine testing methods have garnered significant attention worldwide.

[0003] Internationally, the application of urine iodine analysis technology has primarily been moving toward large, computerized precision instruments, leveraging their advanced technology to achieve high precision and accuracy. Examples include ICP-MS and HPLC. Domestically, however, urine iodine analysis technology is primarily tailored to my country's national conditions, utilizing small and medium-sized equipment and employing physical, chemical, or combined techniques. According to the national standard WST 107-2006, "Determination of Iodine in Urine by Arsenic-Cerium Catalytic Spectrophotometry," urine iodine testing in China currently typically involves a digester, water bath, and UV spectrophotometer to complete the urine and water iodine assays. The stringent time constraints associated with these assays increase the operator's difficulty in operating the three instruments, complicating the accuracy and reproducibility of test data. Summary of the Invention

[0004] The present invention provides a fully automatic urine iodine analyzer and a sample analysis method, which avoids errors caused by human participation and greatly improves the convenience and accuracy of the test.

[0005] In order to achieve the above object, the technical solution adopted by the present invention is:

[0006] A fully automatic urine iodine meter, characterized in that: a mobile platform is arranged on an aluminum profile frame, an ultraviolet detector and a water-cooled heater are arranged in the three-dimensional moving space of the mobile platform, the mobile platform provides reagents to the digestion tube on the water-cooled heater through an injection needle, and the mobile platform also provides test samples to the ultraviolet detector through the injection needle, and the ultraviolet detector detects the light intensity values ​​of the sample and the reference solution;

[0007] The ultraviolet detector includes an ultraviolet light source, an optical path box and a silicon photocell detector. The inlet end of the optical path box is provided with a plurality of ultraviolet light sources, the outlet end of the optical path box is provided with the silicon photocell detector, and the optical path box is provided with a cuvette detection pool and a reference solution detection pool;

[0008] The optical path box is provided with a lens, a light shielding mirror, a spectroscope, the cuvette detection pool, a first filter and the silicon photocell detector in sequence according to the direct light path, and the reference solution detection pool, the second filter and the silicon photocell detector are provided in sequence on the refracted light path of the spectroscope;

[0009] The ultraviolet light emitted by the ultraviolet light source passes through the lens to correct the divergent light into parallel light. After the parallel light passes through the light-shielding mirror, a beam of transmitted light at the center is retained. After the transmitted light passes through the beam splitter, it is divided into two light paths, one of which is a direct light path and the other is a refracted light path.

[0010] The transmitted light on one of the direct light paths is incident on the cuvette detection pool, and the ultraviolet light emitted by the sample in the cuvette detection pool is filtered by the first filter and then irradiated into the silicon photocell detector to detect the light intensity value of the sample;

[0011] The transmitted light on the other refracted light path is incident on the reference solution detection cell. The ultraviolet light emitted by the sample in the reference solution detection cell is filtered by the second filter and then irradiated into the silicon photocell detector to detect the light intensity value of the reference solution. By comparing the light intensity values ​​of the sample and the reference solution, the concentration value of the sample can be analyzed;

[0012] The water-cooled heater includes a heating block and a constant temperature box. The heating block is arranged in the constant temperature box. A plurality of digestion holes are arranged in the heating block for inserting the digestion tube. A cooling channel is arranged at the bottom of the heating block. The outlet and the inlet of the cooling channel are connected in sequence through a circulation pipeline to a parallel flow radiator, a cooling water tank and a cooling pump. A cooling fan is arranged on the parallel flow radiator for air cooling. A coolant is injected into the cooling water tank. The cooling pump circulates the coolant in the pipeline to cool the heating block. ;

[0013] The mobile platform includes an X-axis arm, a Y-axis arm and a Z-axis arm, which are sequentially connected by transmission and can move horizontally, longitudinally and vertically;

[0014] The Z-axis arm contains a sealed space, and a Z-axis slider is provided in the Z-axis arm. The Z-axis slider is connected to the driving device through a Z-axis synchronous belt for vertical movement of the Z-axis slider. The Z-axis slider is fixedly connected to the injection needle. A bottom sealing plate is provided at the bottom end of the Z-axis arm, and a pinhole is provided on the bottom sealing plate. The pinhole has a conical structure, and the positive pressure gas introduced from the outside is used to blow away the reagent stuck on the outer wall of the injection needle and flow out through the pinhole, and the injection needle moves vertically through the pinhole.

[0015] The fully automatic urine iodine meter, wherein: a ventilation valve is provided on the cooling water tank, and a waterproof and breathable membrane is provided inside the ventilation valve to balance the pressure difference between the inside and outside of the cooling water tank.

[0016] The fully automatic urine iodine meter, wherein: a No. 1 needle and a No. 2 needle are independently arranged in the injection needle, the No. 1 needle is connected to several reagent bottles through a ceramic pump and a rotary valve, the No. 1 needle is used for adding reagents, the No. 2 needle is connected to a peristaltic pump, and the No. 2 needle is used to transfer the sample to the cuvette detection pool or to bubble and mix the sample.

[0017] A sample analysis method for a fully automatic urine iodine analyzer is characterized by comprising the following steps:

[0018] Step 1: Inject an appropriate amount of coolant into the cooling water tank, and inject the coolant into the cooling channel through the circulation pipeline to ensure that the coolant temperature in the cooling channel and the cooling water tank is consistent;

[0019] Step 2: Insert several digestion tubes on the heating block, and use the mobile platform to add reagents into the digestion tubes through the injection needle. Use the No. 1 needle to add 0.25 ml of iodine standard solution and urine sample into all digestion tubes respectively;

[0020] Step 3, the No. 1 needle sequentially adds 1 ml of ammonium persulfate solution to the digestion tube, and the No. 2 needle separately adds bubbling to the digestion tube to mix;

[0021] Step 4: After bubbling and mixing, the heating block is heated to 100°C according to the test requirements and digested at a constant temperature for 60 minutes;

[0022] Step 5: After digestion is completed, start the cooling fan and cooling pump. The coolant flows to perform heat exchange, cooling the sample in the digestion tube to the set temperature of 30°C and maintaining a constant temperature.

[0023] Step 6, the No. 1 needle adds 2.5 ml of arsenite solution to the first batch of 30 digestion tubes at intervals of 30 seconds. After each digestion tube is added, the No. 2 needle bubble-mixes the sample. After the sample in the first digestion tube is mixed, the 15-minute timer starts;

[0024] Step 7: After the first digestion tube has been allowed to stand for 15 minutes, 0.3 ml of ammonium cerium sulfate solution is added to each of the first 30 digestion tubes using the No. 1 needle at 30 second intervals, and the solution is mixed by bubbling using the No. 2 needle. A 30-minute timer is then started.

[0025] Step 8: After the first digestion tube has been allowed to stand for 30 minutes, the second needle, in cooperation with the mobile platform, draws 3 ml of sample from the first digestion tube every 30 seconds, moves to the top of the reference solution detection pool, and injects 2.5 ml of sample into the detection pool for photometric measurement;

[0026] Step 9: After the photometric measurement is completed, the second needle and the reference solution detection pool are cleaned, and the sample in the next digestion tube is prepared for detection;

[0027] Step 10: After the samples in the first batch of 30 digestion tubes are tested in sequence, the next batch of samples can be processed and analyzed using the same method according to steps 6 to 9 above.

[0028] The beneficial effects of the present invention are as follows: the multiple functions of digestion, water bath and detection are integrated into one, and the entire test process is highly automated, thus avoiding errors caused by human participation and greatly improving the convenience and accuracy of the test. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 It is a structural diagram of the fully automatic urine iodine meter.

[0030] Figure 2 This is a structural diagram of the fully automatic urine iodine meter from another perspective.

[0031] Figure 3 This is a schematic diagram of the internal structure of the fully automatic urine iodine meter.

[0032] Figure 4 It is a structural diagram of the aluminum profile skeleton.

[0033] Figure 5 A structural diagram of the mobile platform.

[0034] Figure 6 Schematic diagram of the cross-sectional structure of the Z-axis arm.

[0035] Figure 7 This is a partial enlarged view of the Z-axis arm.

[0036] Figure 8 Schematic diagram of the injection needle structure.

[0037] Figure 9 Schematic diagram of the heating block structure.

[0038] Figure 10 Schematic diagram of the cross-sectional structure of the heating block.

[0039] Figure 11 Schematic diagram of the cooling channel structure.

[0040] Figure 12 It is a structural diagram of the water-cooled heater.

[0041] Figure 13 This is a structural diagram of the water-cooled heater from another perspective.

[0042] Figure 14Schematic diagram of the structure of the UV detector.

[0043] Figure 15 Schematic diagram of the optical path of the UV detector.

[0044] Figure 16 This is a schematic diagram of the pipeline flow direction of needle No. 1 and needle No. 2.

[0045] Explanation of the accompanying drawings: 1-UV detector; 2-water-cooled heater; 3-movable platform; 4-aluminum profile frame; 5-X-axis arm; 6-Y-axis arm; 7-Z-axis arm; 8-Z-axis slider; 9-Z-axis synchronous belt; 10-injection needle; 11-bottom sealing plate; 12-pinhole; 13-heating block; 14-cooling channel; 15-digestion hole; 16-constant temperature box; 17-cooling fan; 18-parallel flow radiator; 19-cooling water tank; 20-breathable valve; 21-cooling pump; 22-UV light source; 23-cuvette detection cell; 24-silicon photocell detector; 25-lens; 26-shading mirror; 27-spectrometer; 28-first filter; 29-reference solution detection cell; 30-second filter; 31-No. 1 needle; 32-No. 2 needle. DETAILED DESCRIPTION

[0046] like Figures 1 to 16 The fully automatic urine iodine meter shown includes an ultraviolet detector 1, a water-cooled heater 2 and a moving platform 3.

[0047] The mobile platform 3 is arranged on the aluminum profile frame 4, and the ultraviolet detector 1 and the water-cooled heater 2 are arranged in the three-dimensional moving space of the mobile platform 3. The mobile platform 3 provides reagents to the digestion tube on the water-cooled heater 2 through the injection needle 10. The mobile platform 3 also provides test samples to the ultraviolet detector 1 through the injection needle 10. The ultraviolet detector 1 detects the light intensity values ​​of the sample and the reference solution.

[0048] The ultraviolet detector 1 includes an ultraviolet light source 22, an optical path box and a silicon photocell detector 24. A plurality of ultraviolet light sources 22 are provided at the inlet end of the optical path box, and the silicon photocell detector 24 is provided at the outlet end of the optical path box.

[0049] The lens 25, the shading mirror 26, the spectrometer 27, the cuvette detection pool 23, the first filter 28 and the silicon photocell detector 24 are arranged in sequence in the optical path box according to the direct light path, and the reference liquid detection pool 29, the second filter 30 and the silicon photocell detector 24 are also arranged in sequence on the refracted light path of the spectrometer 27.

[0050] The ultraviolet light emitted by the ultraviolet light source 22 passes through the lens 25, which can correct the divergent light into parallel light. After the parallel light passes through the shading mirror 26, a beam of transmitted light is retained at the center position. The retained transmitted light is divided into two light paths after passing through the beam splitter 27, one of which is a direct light path and the other is a refracted light path.

[0051] The transmitted light on a direct light path enters the cuvette detection pool 23 , and the ultraviolet light emitted by the sample in the cuvette detection pool 23 is filtered by the first filter 28 and then irradiated into the silicon photocell detector 24 to detect the light intensity value of the sample.

[0052] The transmitted light on the other refracted light path is incident on the reference liquid detection pool 29. The ultraviolet light emitted by the sample in the reference liquid detection pool 29 is filtered by the second filter 30 and then irradiated into the silicon photocell detector 24 to detect the light intensity value of the reference liquid. By comparing the light intensity values ​​of the sample and the reference liquid, the concentration value of the sample can be analyzed.

[0053] The water-cooled heater 2 includes a heating block 13 and a constant temperature box 16. The heating block 13 is arranged in the constant temperature box 16. Several digestion holes 15 are arranged in the heating block 13 for inserting the digestion tube. A cooling channel 14 is arranged at the bottom of the heating block 13. The outlet and the inlet of the cooling channel 14 are connected in sequence through a circulation pipeline to a parallel flow radiator 18, a cooling water tank 19 and a cooling pump 21. A cooling fan 17 is provided on the parallel flow radiator 18 for air cooling. Coolant is injected into the cooling water tank 19. A breathable valve 20 is provided on the cooling water tank 19. A waterproof and breathable membrane is provided in the breathable valve 20 to balance the internal and external pressure difference of the cooling water tank 19. The cooling pump 21 circulates the coolant in the pipeline to cool the heating block 13.

[0054] The mobile platform 3 includes an X-axis arm 5, a Y-axis arm 6 and a Z-axis arm 7, which are sequentially connected in a transmission manner and can move horizontally, longitudinally and vertically.

[0055] The Z-axis arm 7 is configured as a sealed space, and a Z-axis slider 8 is provided inside the Z-axis arm 7. The Z-axis slider 8 is connected to the driving device through a Z-axis synchronous belt 9 for vertical movement of the Z-axis slider 8. The Z-axis slider 8 is fixedly connected to the injection needle 10. A bottom sealing plate 11 is provided at the bottom end of the Z-axis arm 7, and a pinhole 12 is provided on the bottom sealing plate 11. The pinhole 12 has a conical structure, and the positive pressure gas introduced from the outside can blow away the reagent stuck on the outer wall of the injection needle 10. The blown-away reagent flows out of the Z-axis arm 7 through the pinhole 12, and the injection needle 10 moves vertically through the pinhole 12.

[0056] The injection needle 10 is independently provided with a No. 1 needle 31 and a No. 2 needle 32 .

[0057] The No. 1 needle 31 is connected to several reagent bottles through a ceramic pump and a rotary valve. After selecting the reagent through the rotary valve, the ceramic pump is started and the reagent is added as required.

[0058] The second needle 32 is connected to the peristaltic pump, and the second needle 32 is used for sample transfer or bubbling and mixing the sample.

[0059] When the sample is transferred, the peristaltic pump reverses, the second needle 32 sucks the sample out of the digestion tube, and moves to the top of the cuvette detection pool 23 under the control of the mobile platform 3. The peristaltic pump rotates forward to push the sucked sample into the cuvette detection pool 23.

[0060] When bubbling and mixing the sample, the peristaltic pump reverses and inhales a certain amount of air. Then, it moves into the digestion tube under the control of the mobile platform 3. The peristaltic pump rotates forward to discharge the inhaled air through the second needle 32. The discharged bubbles can mix the sample in the digestion tube.

[0061] A sample analysis method for a fully automatic urine iodine analyzer is characterized by comprising the following steps:

[0062] Step 1: Inject an appropriate amount of coolant into the cooling water tank 19, and inject the coolant into the cooling channel 14 through the circulation pipeline to ensure that the coolant temperature in the cooling channel 14 and the cooling water tank 19 is consistent;

[0063] Step 2: Insert several digestion tubes into the heating block 13, and the mobile platform 3 adds reagents into the digestion tubes through the injection needle 10. The No. 1 needle 31 adds 0.25 ml of iodine standard solution and urine sample into all digestion tubes respectively;

[0064] Step 3, the No. 1 needle 31 adds 1 ml of ammonium persulfate solution to the digestion tube in sequence, and the No. 2 needle 32 adds bubbling to the digestion tube to mix;

[0065] Step 4: After bubbling and mixing, the heating block 13 is heated to 100° C. according to the test requirements and digested at a constant temperature for 60 minutes;

[0066] Step 5: After digestion is completed, the cooling fan 17 and the cooling pump 21 are started, and the coolant flows to perform heat exchange, cooling the sample in the digestion tube to a set temperature of 30°C and maintaining a constant temperature;

[0067] Step 6, the No. 1 needle 31 adds 2.5 ml of arsenite solution to the first batch of 30 digestion tubes at intervals of 30 seconds. After each digestion tube is added, the No. 2 needle 32 bubble mixes the sample respectively. After the sample in the first digestion tube is mixed, the 15-minute timer starts;

[0068] Step 7: After the first digestion tube has been allowed to stand for 15 minutes, the No. 1 needle 31 adds 0.3 ml of the ammonium cerium sulfate solution to each of the first 30 digestion tubes at 30 second intervals, and the No. 2 needle 32 performs bubbling mixing, and simultaneously starts a 30-minute timer;

[0069] Step 8: After the first digestion tube has been left to stand for 30 minutes, the second needle 32, in cooperation with the mobile platform 3, draws 3 ml of sample from the first digestion tube every 30 seconds, moves it to the top of the reference solution detection pool 29, and injects 2.5 ml of sample into the detection pool for photometric measurement;

[0070] Step 9: After the photometric measurement is completed, the second needle 32 and the reference solution detection pool 29 are cleaned, and the sample in the next digestion tube is prepared for detection;

[0071] Step 10: After the samples in the first batch of 30 digestion tubes are tested in sequence, the next batch of samples can be processed and analyzed using the same method according to steps 6 to 9 above.

[0072] Advantages of the present invention:

[0073] It integrates multiple functions of digestion, water bath and detection, and the entire test process is highly automated, avoiding errors caused by human participation and greatly improving the convenience and accuracy of the test.

[0074] The above description is only illustrative of the present invention and not restrictive. Those skilled in the art will understand that many modifications, changes or equivalents may be made without departing from the spirit and scope defined by the claims, and all of them will fall within the scope of protection of the present invention.

Claims

1. A fully automatic urine iodine meter, characterized by: The mobile platform (3) is arranged on the aluminum profile frame (4), and an ultraviolet detector (1) and a water-cooled heater (2) are arranged in the three-dimensional moving space of the mobile platform (3). The mobile platform (3) provides a reagent to the digestion tube on the water-cooled heater (2) through an injection needle (10). The mobile platform (3) also provides a test sample to the ultraviolet detector (1) through the injection needle (10). The ultraviolet detector (1) detects the light intensity value of the sample and the reference solution. The ultraviolet detector (1) comprises an ultraviolet light source (22), an optical path box and a silicon photocell detector (24), wherein a plurality of ultraviolet light sources (22) are arranged at the inlet end of the optical path box, and the silicon photocell detector (24) is arranged at the outlet end of the optical path box, and a cuvette detection pool (23) and a reference solution detection pool (29) are arranged on the optical path box; The optical path box is provided with a lens (25), a light shielding mirror (26), a spectroscope (27), the cuvette detection pool (23), a first filter (28) and the silicon photocell detector (24) in sequence according to the direct light path, and the reference solution detection pool (29), the second filter (30) and the silicon photocell detector (24) are provided in sequence on the refracted light path of the spectroscope (27); The ultraviolet light emitted by the ultraviolet light source (22) passes through the lens (25) to correct the divergent light into parallel light. After the parallel light passes through the light shielding mirror (26), a beam of transmitted light at the center is retained. After the transmitted light passes through the beam splitter (27), it is divided into two light paths, one of which is a direct light path and the other is a refracted light path. The transmitted light on the direct light path is incident on the cuvette detection pool (23), and the ultraviolet light emitted by the sample in the cuvette detection pool (23) is filtered by the first filter (28) and then irradiated into the silicon photocell detector (24) to detect the light intensity value of the sample; The transmitted light on the other refracted light path is incident on the reference solution detection pool (29), and the ultraviolet light emitted by the sample in the reference solution detection pool (29) is filtered by the second filter (30) and then irradiated into the silicon photocell detector (24), detecting the light intensity value of the reference solution. By comparing the light intensity values ​​of the sample and the reference solution, the concentration value of the sample can be analyzed; The water-cooled heater (2) includes a heating block (13) and a constant temperature box (16), wherein the heating block (13) is arranged in the constant temperature box (16), and a plurality of digestion holes (15) are arranged in the heating block (13) for inserting the digestion tube, and a cooling channel (14) is arranged at the bottom of the heating block (13), and the outlet and the inlet of the cooling channel (14) are connected to the parallel flow radiator (18), the cooling water tank (19) and the cooling pump (21) in sequence through a circulation pipeline, and a cooling fan (17) is arranged on the parallel flow radiator (18) for air cooling, and a coolant is injected into the cooling water tank (19), and the cooling pump (21) circulates the coolant in the pipeline for cooling the heating block (13); The mobile platform (3) comprises an X-axis arm (5), a Y-axis arm (6) and a Z-axis arm (7), which are sequentially connected in a transmission manner and are capable of lateral movement, longitudinal movement and vertical movement; The Z-axis arm (7) has a sealed space inside. A Z-axis slider (8) is provided inside the Z-axis arm (7). The Z-axis slider (8) is connected to the driving device through a Z-axis synchronous belt (9) for vertical movement of the Z-axis slider (8). The Z-axis slider (8) is fixedly connected to the injection needle (10). A bottom sealing plate (11) is provided at the bottom end of the Z-axis arm (7). A pinhole (12) is provided on the bottom sealing plate (11). The pinhole (12) has a conical structure. Positive pressure gas introduced from the outside is used to blow away the reagent adhering to the outer wall of the injection needle (10) and flow out through the pinhole (12). The injection needle (10) moves vertically through the pinhole (12).

2. The fully automatic urine iodine meter according to claim 1, wherein: A breathable valve (20) is provided on the cooling water tank (19), and a waterproof breathable membrane is provided inside the breathable valve (20) to balance the pressure difference between the inside and outside of the cooling water tank (19).

3. The fully automatic urine iodine meter according to claim 1, wherein: A No. 1 needle (31) and a No. 2 needle (32) are independently arranged in the injection needle (10), and the No. 1 needle (31) is connected to a plurality of reagent bottles through a ceramic pump and a rotary valve. The No. 1 needle (31) is used for adding reagents, and the No. 2 needle (32) is connected to a peristaltic pump. The No. 2 needle (32) is used for transferring samples to the cuvette detection pool (23) or bubbling and mixing the samples.

4. A sample analysis method for a fully automatic urine iodine analyzer, characterized in that: The fully automatic urine iodine meter according to claim 3 comprises the following steps: Step 1: inject an appropriate amount of coolant into the cooling water tank (19), and inject the coolant into the cooling channel (14) through the circulation pipeline to ensure that the coolant temperature in the cooling channel (14) and the cooling water tank (19) is consistent; Step 2: Insert several digestion tubes into the heating block (13), and use the mobile platform (3) to add reagents into the digestion tubes through the injection needle (10). Use the No. 1 needle (31) to add 0.25 ml of iodine standard solution and urine sample into all digestion tubes respectively; Step 3, the No. 1 needle (31) adds 1 ml of ammonium persulfate solution to the digestion tube in turn, and the No. 2 needle (32) adds bubbling to the digestion tube to mix; Step 4: After the bubbling and mixing are completed, the heating block (13) is heated to 100°C according to the test requirements and digested at a constant temperature for 60 minutes; Step 5: After digestion is completed, the cooling fan (17) and the cooling pump (21) are started, and the coolant is flowed to perform heat exchange, cooling the sample in the digestion tube to a set temperature of 30°C and maintaining a constant temperature; Step 6, the No. 1 needle (31) adds 2.5 ml of arsenite solution to the first batch of 30 digestion tubes at intervals of 30 seconds. After each digestion tube is filled with liquid, the No. 2 needle (32) bubble-mixes the samples. After the mixing of the sample in the first digestion tube is completed, the timer starts to count for 15 minutes; Step 7, after the first digestion tube has been left to stand for 15 minutes, the No. 1 needle (31) adds 0.3 ml of the ammonium cerium sulfate solution to each of the first 30 digestion tubes at intervals of 30 seconds, and the No. 2 needle (32) performs bubbling mixing, and simultaneously starts timing for 30 minutes; Step 8: After the first digestion tube is left to stand for 30 minutes, the second needle (32) draws 3 ml of sample from the first digestion tube with the cooperation of the mobile platform (3) every 30 seconds, moves it to the top of the reference solution detection pool (29), and injects 2.5 ml of sample into the detection pool for photometric measurement; Step 9: After the photometric measurement is completed, the second needle (32) and the reference solution detection pool (29) are cleaned, and the sample in the next digestion tube is prepared for detection; Step 10: After the samples in the first batch of 30 digestion tubes are tested in sequence, the next batch of samples are processed and analyzed using the same method according to steps 6 to 9 above.

Images