A feed additive for promoting reproduction of pregnant sows, its preparation method and application

Abstract

The present invention relates to a feed additive for promoting reproduction of pregnant sows, a preparation method thereof and an application thereof. The components of the feed additive for promoting reproduction of pregnant sows include: a fermented Chinese herbal medicine complex, a compound fat powder, ergothioneine, yeast selenium and a carrier material; the raw materials for preparing the compound fat powder include glycerol butyrate, lipoic acid, nicotinamide and a wall material; the fermented Chinese herbal medicine complex is a product obtained by fermenting a Chinese herbal medicine fermentation substrate with a compound bacterium and a compound enzyme. This feed additive solves the problems that pregnant sows in the previous stage have follicular cysts, the quality of oocytes decreases, embryonic development is damaged, resulting in high rates of miscarriage, premature birth, malformation rate, mummy rate, etc. due to oxidative stress and other reasons, thereby significantly improving the reproductive performance of pregnant sows.

Description

Technical Field

[0001] The present invention belongs to the technical field of pig breeding, and relates to a feed additive for promoting the reproduction of pregnant sows, its preparation method and application, and particularly relates to a feed additive for promoting the reproduction of pregnant sows that can increase the number of live piglets born, reduce the number of malformed piglets born and the number of mummified piglets, and its preparation method and application. Background Art

[0002] In recent years, the pig breeding industry in China has developed rapidly. However, sow reproductive disorders are still widespread in pig farms. Sow reproductive disorders lead to low production performance of sows, fewer piglets born, and a high stillbirth rate of piglets, which cause serious harm to the healthy development of China's pig breeding industry, result in significant economic losses, and have become the main problem restricting the development of China's pig breeding industry.

[0003] The developmental quality of oocytes and early embryos is a key factor affecting the reproductive performance of sows. However, during pregnancy, due to significant physiological state and nutritional metabolism changes in the body, sows are induced to generate excessive free radicals, leading to oxidative stress. Oxidative stress can cause disorders in ovarian endocrine function, resulting in follicular cysts, and further lead to a serious decline in the quality of oocytes and embryos, which seriously endangers the health and reproductive performance of sows. Moreover, an increase in reactive oxygen species or a decrease in antioxidant capacity in follicular fluid can reduce the fertilization rate of oocytes, resulting in sow abortion, premature birth, fewer piglets born, and more weak piglets.

[0004] In current production practice, it is often through directly adding antioxidants to improve the feed quality and reduce oxidative stress in pregnant sows. For example, CN108850615A discloses a feed for late pregnancy sows and a feeding method. The weight ratio of the feed is: 55 - 65 parts of corn, 15 - 25 parts of soybean meal, 5 - 10 parts of wheat bran, 1 - 2 parts of soybean oil, 1 - 2 parts of glucose, 0.5 - 1 part of table salt, 1 - 2 parts of stone powder, 1 - 2 parts of calcium hydrogen phosphate, 0.3 - 0.5 part of lysine, 0.4 - 0.8 part of threonine, 4 - 7 parts of dietary fiber, 2 - 4 parts of plant extract, 0.1 - 0.3 part of choline chloride, and 3 - 4 parts of sow compound premix.

[0005] However, there are a wide variety of antioxidants on the market, with complex and diverse components, and they have certain toxic and side effects, resulting in the failure to fully exert the reproductive performance of pregnant sows. Therefore, it is very necessary to develop a green and safe product that can effectively relieve oxidative stress in pregnant sows and improve the reproductive performance of pregnant sows. Summary of the Invention

[0006] Aiming at the deficiencies of the prior art, the purpose of the present invention is to provide a feed additive for promoting the reproduction of pregnant sows, its preparation method and application, and particularly provide a feed additive for promoting the reproduction of pregnant sows that can increase the number of live piglets born, reduce the number of malformed piglets born and the number of mummified piglets, and its preparation method and application.

[0007] To achieve the object of the present invention, the following technical solutions are adopted in the present invention:

[0008] In a first aspect, the present invention provides a feed additive for promoting reproduction of pregnant sows, and the components of the feed additive for promoting reproduction of pregnant sows include: a fermented Chinese herbal medicine complex, a compound fat powder, ergothioneine, yeast selenium, and a carrier material;

[0009] The preparation raw materials of the compound fat powder include glycerol butyrate, lipoic acid, nicotinamide, and wall materials;

[0010] The fermented Chinese herbal medicine complex is a product obtained by fermenting Chinese herbal medicine substrates with a compound bacterium and a compound enzyme.

[0011] In view of the physiological characteristics and nutritional requirements of pregnant sows, the present invention has developed a feed additive, which combines four functional components, namely, a fermented Chinese herbal medicine complex, a compound fat powder, ergothioneine, and yeast selenium. The rich active nutrients cooperate with each other to jointly promote the development of follicles and embryos.

[0012] An increase in the level of HCY (homocysteine) in the body will cause early embryonic death, fetal malformation, and embryo implantation failure. Among them, the fermented Chinese herbal medicine complex is rich in various active substances, which has the effects of improving immunity, antioxidation, antivirus, etc. It can not only inhibit oocyte apoptosis, improve oxidative stress in sows, and enhance the antioxidant capacity of the body, but also inhibit the excessive accumulation of HCY (homocysteine), relieve the toxic effect of HCY on follicle development, improve the quality of oocytes, and promote embryo development. Among them, lipoic acid can participate in the remethylation reaction of HCY and reduce the content of HCY in pregnancy plasma. It cooperates with the fermented Chinese herbal medicine complex and has a synergistic effect in relieving the toxic effect of HCY on follicle development and improving the quality of oocytes.

[0013] Among them, ergothioneine can effectively scavenge free radicals in the uterus of sows, relieve oxidative stress in pregnant sows, avoid embryo loss, and thus promote embryo development; among them, yeast selenium can not only provide nutrients for embryo development, but also participate in the antioxidant process of the body through selenoprotein, thereby enhancing the antioxidant stress ability of pregnant sows; therefore, ergothioneine and yeast selenium can cooperate with the fermented Chinese herbal medicine complex, and the three have a synergistic effect in relieving oxidative stress in pregnant sows, avoiding follicular cysts, and improving the quality of oocytes.

[0014] Among them, nicotinamide can reverse abnormal mitochondrial function in the body, reduce the production of reactive oxygen species, avoid DNA methylation during embryo development, and further assist in promoting embryo development. Among them, glycerol butyrate can not only provide energy for the rapid development of embryos, but also regulate histone acetylation and methylation in the body to promote early embryo development.

[0015] In addition, since the effective ingredients of Chinese herbal medicines are mostly distributed in cells and intercellular spaces, and the cell walls are mainly composed of macromolecules such as cellulose, hemicellulose, and pectin, the fermented Chinese herbal medicine complex used in the present invention is a product obtained by fermenting Chinese herbal medicine fermentation substrates with composite bacteria and composite enzymes. It can effectively digest plant cell walls, release active substances, and produce new enzyme and bacterial active substances, thereby playing a better role.

[0016] Preferably, the components of the pregnant sow reproduction promoting feed additive include, by mass, 200-500 parts of fermented Chinese herbal medicine complex, 50-200 parts of compound fat powder, 1-10 parts of ergothioneine, 10-25 parts of yeast selenium and 400-800 parts of carrier material.

[0017] Based on the potential interaction relationship between the above-mentioned various components, the present invention finds that when the various components are compounded in the above-mentioned specific mass ratio, they have better effects of improving oocyte quality, promoting embryo development, and improving reproductive performance.

[0018] The mass proportions of the fermented Chinese herbal compound can be 200 parts, 220 parts, 250 parts, 280 parts, 300 parts, 350 parts, 400 parts, 450 parts, 500 parts, etc.

[0019] The mass proportions of the compound fat powder can be selected to be 50 parts, 80 parts, 100 parts, 120 parts, 140 parts, 150 parts, 170 parts, 180 parts, 200 parts, etc.

[0020] The mass fraction of the ergothioneine can be selected from 1 part, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, etc.

[0021] The mass proportion of the yeast selenium can be selected to be 10 parts, 15 parts, 20 parts, 25 parts, etc.

[0022] The mass fraction of the carrier material can be selected from 400 parts, 450 parts, 500 parts, 600 parts, 700 parts, 800 parts, etc.

[0023] Other specific point values within the above numerical ranges can be selected and will not be described in detail here.

[0024] Preferably, the carrier material comprises rice husk powder.

[0025] Preferably, the raw materials for preparing the composite fat powder include 30-50 parts of butyric acid glyceride, 1-10 parts of lipoic acid, 1-10 parts of niacinamide and 40-70 parts of wall materials in parts by mass.

[0026] The mass parts of the glycerol butyrate can be selected as 30 parts, 32 parts, 35 parts, 38 parts, 40 parts, 42 parts, 45 parts, 46 parts, 48 parts, 50 parts, etc.

[0027] The mass parts of the lipoic acid can be selected as 1 part, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, etc.

[0028] The mass parts of the nicotinamide can be selected as 1 part, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, etc.

[0029] The mass parts of the wall material can be selected as 40 parts, 42 parts, 45 parts, 48 parts, 50 parts, 52 parts, 55 parts, 60 parts, 65 parts, 70 parts, etc.

[0030] Other specific point values within the above numerical ranges can be selected and will not be elaborated one by one here.

[0031] Preferably, the wall material includes maltodextrin and casein.

[0032] Preferably, the compound fat powder is obtained by spray drying after mixing each preparation raw material with water.

[0033] Preferably, the compound bacteria is a combination of yeast and Bacillus subtilis.

[0034] The fermentation Chinese herbal medicine complex used in the present invention more preferably uses the combination of yeast and Bacillus subtilis to ferment the substrate, so that the obtained product has more excellent antioxidant ability of the organism and the ability to inhibit the excessive accumulation of HCY. Further, the combined fermentation is carried out in the following specific mass ratio to maximize the effect.

[0035] Preferably, the ratio of the viable count of yeast to that of Bacillus subtilis is 1:(1 - 4), such as 1:1, 1:2, 1:3, 1:4, etc.; the total viable count of the compound bacteria is greater than 1×10 9 CFU / g, such as 1×10 9 CFU / g, 5×10 9 CFU / g, 1×10 10 CFU / g, 5×10 10 CFU / g, 1×10 11 CFU / g, etc.

[0036] Preferably, the dosage of the compound bacteria is 1 - 5% of the mass of the Chinese herbal medicine fermentation substrate, such as 1%, 2%, 3%, 4%, 5%, etc.

[0037] Other specific point values within the above numerical ranges can be selected and will not be elaborated one by one here.

[0038] Preferably, the complex enzyme is a combination of xylanase, cellulase and neutral protease.

[0039] The fermented Chinese herbal medicine complex used in the present invention more preferably ferments the substrate in a combination of xylanase, cellulase and neutral protease, so that the obtained product has better antioxidant capacity of the body and the ability to inhibit the excessive accumulation of HCY. Further, the combined fermentation is carried out in the following specific mass ratio to maximize the effect.

[0040] Preferably, the mass ratio of the xylanase, cellulase and neutral protease is (1-3):(2-4):(2-3), such as 1:2:2, 1:2:3, 1:3:2, 1:3:3, 2:2:2, 2:2:3, 2:3:2, 2:3:3, 3:2:2, 3:2:3, 3:3:2, 3:2:3, 1:4:2, 1:4:3, 2:4:2, 2:4:3, 3:4:2, 3:4:3, etc.

[0041] Preferably, the dosage of the complex enzyme is 0.5-2% of the mass of the Chinese herbal medicine fermentation substrate, such as 0.5%, 0.8%, 1%, 1.2%, 1.5%, 1.7%, 2%, etc.

[0042] Other specific point values within the above numerical ranges can be selected and will not be elaborated one by one here.

[0043] Preferably, the Chinese herbal medicine fermentation substrate is a combination of sage, magnolia officinalis, raspberry, roselle and quercetin.

[0044] The fermented Chinese herbal medicine complex used in the present invention more preferably uses the above combination of five Chinese herbal medicines as the fermentation substrate, and cooperates with the complex bacteria and complex enzyme with the above specific formula, so that the final product has the best effect in the comprehensive ability of antioxidant of the body and inhibiting the excessive accumulation of HCY.

[0045] Preferably, the Chinese herbal medicine fermentation substrate is a combination of 5-10 parts of sage, 1-5 parts of magnolia officinalis, 1-10 parts of raspberry, 1-5 parts of roselle and 1-10 parts of quercetin by mass.

[0046] The mass of sage can be selected as 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, etc.; the mass of magnolia officinalis can be selected as 1 part, 2 parts, 3 parts, 4 parts, 5 parts, etc.; the mass of raspberry can be selected as 1 part, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, etc.; the mass of roselle can be selected as 1 part, 2 parts, 3 parts, 4 parts, 5 parts, etc.; the mass of quercetin can be selected as 1 part, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, etc.

[0047] Other specific point values within the above numerical ranges can be selected and will not be elaborated one by one here.

[0048] Preferably, the fermented Chinese herbal medicine complex is prepared by a preparation method including the following steps:

[0049] (1) Mix the Chinese herbal medicine fermentation substrates, crush them, and sieve them for standby; mix the compound bacteria with water at 30 - 40 °C for oxygenation activation treatment, and then mix with the compound enzyme for standby;

[0050] (2) Mix the obtained bacteria - enzyme solution with the treated fermentation substrates, carry out anaerobic fermentation, and dry to obtain the fermented Chinese herbal medicine complex.

[0051] Preferably, the time for the oxygenation activation treatment is 3 - 5 h, such as 3 h, 3.2 h, 3.5 h, 4 h, 4.2 h, 4.5 h, 4.8 h, 5 h, etc.

[0052] Preferably, the temperature for the anaerobic fermentation is 15 - 40 °C, such as 15 °C, 20 °C, 25 °C, 30 °C, 35 °C, 40 °C, etc.; the time for the anaerobic fermentation is 3 - 7 days, such as 3 days, 4 days, 5 days, 6 days, 7 days, etc. Other specific point values within the above numerical ranges can be selected and will not be elaborated one by one here.

[0053] The preparation process of the fermented Chinese herbal medicine complex involved in the present invention is simple and easy to operate, and is suitable for large - scale industrial production.

[0054] In the second aspect, the present invention provides a preparation method of a feed additive for promoting reproduction of pregnant sows according to the first aspect. The preparation method includes: uniformly mixing the fermented Chinese herbal medicine complex, compound fat powder, ergothioneine, yeast selenium, and carrier material.

[0055] In the third aspect, the present invention provides a feed for promoting reproduction of pregnant sows. The feed for promoting reproduction of pregnant sows contains a basal diet and the feed additive for promoting reproduction of pregnant sows according to the first aspect. The feed additive for promoting reproduction of pregnant sows accounts for 0.1% - 0.5% of the mass of the basal diet, such as 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, etc. Other specific point values within this numerical range can be selected and will not be elaborated one by one here.

[0056] Compared with the prior art, the present invention has the following beneficial effects:

[0057] In view of the physiological characteristics and nutritional requirements of pregnant sows, the present invention has developed a feed additive by compounding four functional ingredients: fermented Chinese herbal medicine complex, compound fat powder, ergothioneine, and yeast selenium. The fermented Chinese herbal medicine complex is rich in various active substances, which can improve immunity, antioxidation, antiviral effects, etc. It can not only inhibit oocyte apoptosis, improve oxidative stress in sows, and enhance the body's antioxidant capacity, but also inhibit the excessive accumulation of HCY (homocysteine), alleviate the toxic effect of HCY on follicular development, improve the quality of oocytes, and promote embryonic development. Among them, lipoic acid can participate in the remethylation reaction of HCY, reduce the content of HCY in pregnancy plasma, and cooperate with the fermented Chinese herbal medicine complex to have a synergistic effect in alleviating the toxic effect of HCY on follicular development and improving the quality of oocytes.

[0058] Among them, ergothioneine can effectively scavenge free radicals in the uterus of sows, alleviate oxidative stress in pregnant sows, avoid embryo loss, and thus promote embryonic development. Yeast selenium can not only provide nutrients for embryonic development, but also participate in the body's antioxidant process through selenoprotein, thereby improving the antioxidant stress ability of pregnant sows. Ergothioneine and yeast selenium can cooperate with the fermented Chinese herbal medicine complex, and the three have a synergistic effect in alleviating oxidative stress in pregnant sows, avoiding follicular cysts, and improving the quality of oocytes.

[0059] Among them, nicotinamide can reverse abnormal mitochondrial function in the body, reduce the production of reactive oxygen species, avoid DNA methylation during embryonic development, and further assist in promoting embryonic development. Glycerol butyrate can not only provide energy for the rapid development of embryos, but also regulate histone acetylation and methylation in the body to promote early embryonic development.

[0060] In summary, this feed additive solves the problems of follicular cysts in sows, decreased oocyte quality, damaged embryonic development, and high rates of miscarriage, premature birth, malformation rate, and mummy rate in pregnant sows due to oxidative stress and other reasons, thereby significantly improving the reproductive performance of pregnant sows. Detailed implementation methods

[0061] To further elaborate on the technical means and effects adopted by the present invention, the following further illustrates the technical solutions of the present invention in combination with the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.

[0062] The raw materials involved in the following preparation examples, examples, and comparative examples can all be obtained through commercial channels or through the content disclosed in the prior art and common general knowledge in combination with the conventional technical methods known to those skilled in the art.

[0063] The yeast powder involved below was purchased from Shandong Siyang Biotechnology Co., Ltd. with a viable count of 5×10 10Product with CFU / g; The Bacillus subtilis powder is a product with a viable count of 1×10 11 CFU / g from Shandong Zhongke Jiayi Biotechnology Co., Ltd.;

[0064] The xylanase is a product with an enzyme activity of 52561 U / g purchased from Shaanxi RanKang Biotechnology Co., Ltd.;

[0065] The cellulase is a product with an enzyme activity of 112842 U / g purchased from Shaanxi RanKang Biotechnology Co., Ltd.;

[0066] The neutral protease is a product with an enzyme activity of 20000 U / g purchased from Shaanxi Chenming Biotechnology Co., Ltd.;

[0067] The ergothioneine is a product with a content of 99% purchased from Xi'an Paifeite Industrial Co., Ltd.;

[0068] The yeast selenium is a product with a selenium content of 2500 ppm purchased from Shandong Siyang Biotechnology Co., Ltd.

[0069] Preparation Example 1-1

[0070] This preparation example provides a fermented Chinese herbal medicine complex, and its preparation method is as follows:

[0071] (1) Mix sage, magnolia bark, raspberry, roselle and quercetin raw materials with a mass ratio of 2:1:2:1:2, pass through a 40-mesh sieve, and reserve as a fermentation substrate;

[0072] (2) Mix the yeast powder and Bacillus subtilis powder with a viable count ratio of 1:3 (total viable count greater than 5×10 9 CFU / g), add to 5 times the mass of warm water at 35°C, stir evenly, perform oxygen input activation treatment for 4 h, and then mix with xylanase, cellulase and neutral protease with a mass ratio of 2:3:2. The mass ratio of the total amount of bacterial powder to the total amount of enzyme is 3:1, and reserve as the bacterial enzyme solution;

[0073] (3) First mix the bacterial enzyme solution with an equal mass of water and then add it to the fermentation substrate. The dosage of the compound bacteria is 3% of the mass of the fermentation substrate, and the dosage of the compound enzyme is 1% of the mass of the fermentation substrate. Stir evenly, seal and bag, perform solid-state anaerobic fermentation, ferment at 30°C for 5 days, and dry after fermentation to obtain the fermented Chinese herbal medicine complex.

[0074] Preparation Example 1-2

[0075] This preparation example provides a fermented Chinese herbal medicine complex, and the difference in its preparation method from Preparation Example 1 is only that the compound bacteria are replaced with single yeast powder, but the dosage of the bacterial powder is still 3% of the mass of the fermentation substrate, and other conditions remain unchanged.

[0076] Preparation Example 1-3

[0077] This preparation example provides a fermented Chinese herbal medicine complex. The difference in its preparation method from that of Preparation Example 1 is only that the complex bacteria are replaced with single Bacillus subtilis powder, but the dosage of the bacterial powder is still 3% of the mass of the fermentation substrate, and other conditions remain unchanged.

[0078] Preparation Examples 1-4

[0079] This preparation example provides a fermented Chinese herbal medicine complex. The difference in its preparation method from that of Preparation Example 1 is only that the complex enzyme is replaced with xylanase and cellulase with a mass ratio of 2:3, but the dosage of the complex enzyme is still 1% of the mass of the fermentation substrate, and other conditions remain unchanged.

[0080] Preparation Examples 1-5

[0081] This preparation example provides a fermented Chinese herbal medicine complex. The difference in its preparation method from that of Preparation Example 1 is only that the complex enzyme is replaced with xylanase and neutral protease with a mass ratio of 1:1, but the dosage of the complex enzyme is still 1% of the mass of the fermentation substrate, and other conditions remain unchanged.

[0082] Preparation Examples 1-6

[0083] This preparation example provides a fermented Chinese herbal medicine complex. The difference in its preparation method from that of Preparation Example 1 is only that the complex enzyme is replaced with cellulase and neutral protease with a mass ratio of 3:2, but the dosage of the complex enzyme is still 1% of the mass of the fermentation substrate, and other conditions remain unchanged.

[0084] Preparation Examples 1-7

[0085] This preparation example provides a fermented Chinese herbal medicine complex. The difference in its preparation method from that of Preparation Example 1 is only that the fermentation substrate is different, which is a mixture of Magnolia officinalis, Rubus idaeus, Hibiscus sabdariffa, and Quercus raw materials with a mass ratio of 1:2:1:2, passed through a 40-mesh sieve, and the dosage of the fermentation substrate remains unchanged.

[0086] Preparation Examples 1-8

[0087] This preparation example provides a fermented Chinese herbal medicine complex. The difference in its preparation method from that of Preparation Example 1 is only that the fermentation substrate is different, which is a mixture of Salvia officinalis, Rubus idaeus, Hibiscus sabdariffa, and Quercus raw materials with a mass ratio of 2:2:1:2, passed through a 40-mesh sieve, and the dosage of the fermentation substrate remains unchanged.

[0088] Preparation Examples 1-9

[0089] This preparation example provides a fermented Chinese herbal medicine complex. The difference in its preparation method from that of Preparation Example 1 is only that the fermentation substrate is different, which is a mixture of Salvia officinalis, Magnolia officinalis, Hibiscus sabdariffa, and Quercus raw materials with a mass ratio of 2:1:1:2, passed through a 40-mesh sieve, and the dosage of the fermentation substrate remains unchanged.

[0090] Preparation Examples 1-10

[0091] This preparation example provides a fermented Chinese herbal medicine complex, and its preparation method differs from that of Preparation Example 1 only in that the fermentation substrate is different, which is a mixture of sage, magnolia bark, raspberry and quercetin raw materials in a mass ratio of 2:1:2:2, passed through a 40-mesh sieve, and the amount of the fermentation substrate remains unchanged.

[0092] Preparation Example 1-11

[0093] This preparation example provides a fermented Chinese herbal medicine complex, and its preparation method differs from that of Preparation Example 1 only in that the fermentation substrate is different, which is a mixture of sage, magnolia bark, raspberry, and roselle in a mass ratio of 2:1:2:1, passed through a 40-mesh sieve, and the amount of the fermentation substrate remains unchanged.

[0094] Preparation Example 2-1

[0095] This preparation example provides a composite fat powder, and the preparation method thereof is as follows:

[0096] 40 parts of butyric acid glyceride, 5 parts of lipoic acid, 5 parts of nicotinamide, 30 parts of maltodextrin and 20 parts of casein are mixed and dissolved in 30 parts of water, and the mixture is spray-dried to obtain the product.

[0097] Preparation Example 2-2

[0098] This preparation example provides a composite fat powder, and the preparation method thereof is as follows:

[0099] 40 parts of butyric acid glyceride, 5 parts of nicotinamide, 35 parts of maltodextrin and 20 parts of casein are mixed and dissolved in 30 parts of water, and the mixture is spray-dried to obtain the product.

[0100] Preparation Example 2-3

[0101] This preparation example provides a composite fat powder, and the preparation method thereof is as follows:

[0102] 40 parts of butyric acid glyceride, 5 parts of lipoic acid, 35 parts of maltodextrin and 20 parts of casein are mixed and dissolved in 30 parts of water, and the mixture is spray-dried to obtain the product.

[0103] Example 1

[0104] The present embodiment provides a pregnant sow reproduction promoting feed additive, the components of which are 300 parts of the fermented Chinese herbal compound prepared in Preparation Example 1-1, 100 parts of the compound fat powder prepared in Preparation Example 2-1, 10 parts of ergothioneine, 20 parts of yeast selenium, and 570 parts of rice husk powder in parts by mass. The fermented Chinese herbal compound, the compound fat powder, ergothioneine, the yeast selenium and the rice husk powder are uniformly mixed to obtain the product.

[0105] Example 2

[0106] This example provides a feed additive for promoting reproduction in pregnant sows. Its components are, by mass fraction, 200 parts of the fermented Chinese herbal medicine complex prepared in Preparation Example 1-1, 200 parts of the compound fat powder prepared in Preparation Example 2-1, 5 parts of ergothioneine, 25 parts of yeast selenium, and 570 parts of rice husk powder. Mix the fermented Chinese herbal medicine complex, compound fat powder, ergothioneine, yeast selenium, and rice husk powder evenly to obtain it.

[0107] Example 3-7

[0108] This example provides five feed additives for promoting reproduction in pregnant sows. The difference in their components from those of Example 1 is only that the fermented Chinese herbal medicine complex prepared in Preparation Example 1-1 is respectively and equally replaced by the fermented Chinese herbal medicine complexes prepared in Preparation Example 1-2, Preparation Example 1-3, Preparation Example 1-4, Preparation Example 1-5, and Preparation Example 1-6, and the other components and their contents remain unchanged.

[0109] Example 8-12

[0110] This example provides five feed additives for promoting reproduction in pregnant sows. The difference in their components from those of Example 1 is only that the fermented Chinese herbal medicine complex prepared in Preparation Example 1-1 is respectively and equally replaced by the fermented Chinese herbal medicine complexes prepared in Preparation Example 1-7, Preparation Example 1-8, Preparation Example 1-9, Preparation Example 1-10, and Preparation Example 1-11, and the other components and their contents remain unchanged.

[0111] Example 13-14

[0112] This example provides two feed additives for promoting reproduction in pregnant sows. The difference in their components from those of Example 1 is only that the compound fat powder prepared in Preparation Example 2-1 is respectively and equally replaced by the compound fat powders prepared in Preparation Example 2-2 and Preparation Example 2-3, and the other components and their contents remain unchanged.

[0113] Comparative Example 1

[0114] This comparative example provides a feed additive for promoting reproduction in pregnant sows. The difference in its components from those of Example 1 is only that it does not contain ergothioneine and yeast selenium, and the reduced amounts are distributed to the mass of the fermented Chinese herbal medicine complex prepared in Preparation Example 1-1, and the other components and their contents remain unchanged.

[0115] Comparative Example 2

[0116] This comparative example provides a feed additive for promoting reproduction in pregnant sows. The difference in its components from those of Example 1 is only that it does not contain ergothioneine, and the reduced amount is distributed to the mass of yeast selenium, and the other components and their contents remain unchanged.

[0117] Comparative Example 3

[0118] This comparative example provides a feed additive for promoting reproduction in pregnant sows. The difference in its components from those in Example 1 is only that it does not contain yeast selenium, and the reduced amount is allocated to the mass of ergothioneine, while the other components and their contents remain unchanged.

[0119] Test example

[0120] Using a single-factor experimental design, 162 two-way crossbred sows were selected and randomly divided into 18 treatment groups, including 1 control group and 17 experimental groups. The control group was fed a basal diet (61% corn, 15% soybean meal, 2% fish meal, 18% wheat bran, 4% premix, and the premix was purchased from Guangzhou Aonong Biotechnology Co., Ltd.). The experimental groups were respectively added with 0.2% of the additives prepared in Examples 1-14 and Comparative Examples 1-3 to the basal diet. Each treatment had three replicates, with 3 pigs in each replicate. The experimental period started from breeding until farrowing.

[0121] On the day of farrowing, blood samples were collected from the ear edge vein of the sows. The blood samples were allowed to stand at 25 °C for 10 min and then centrifuged at 3000 r / min for 15 min to prepare serum. The serum was transferred to an Ep tube and stored at -20 °C. The content of HCY in the serum was measured using an ELISA kit purchased from Nanjing Jiancheng Bioengineering Institute, and the specific operation was carried out according to the instructions. The results of the HCY content in each group are shown in Table 1.

[0122] During farrowing, the total number of piglets born, the number of live piglets born, the live piglet birth rate, the number of malformed piglets born, and the number of mummified piglets were recorded. The results of each group are shown in Table 2.

[0123] Table 1

[0124]

[0125]

[0126] From the data results in Table 1, it can be seen that the feed additive involved in the present invention has a significant effect of inhibiting the excessive accumulation of HCY and alleviating the toxic effect of HCY on follicle development, and the fermented Chinese herbal medicine complex and lipoic acid play a major role, and the formula and preparation process of the fermented Chinese herbal medicine complex affect this effect.

[0127] Table 2

[0128]

[0129]

[0130] From the data results in Table 2, it can be seen that the feed additive involved in the present invention has a significant effect of promoting embryo development and improving reproductive performance, and the formula and preparation process of the fermented Chinese herbal medicine complex and the formula of the compound fat powder all have a certain influence on this effect.

[0131] The applicant declares that the present invention uses the above-mentioned embodiments to illustrate a reproductive-promoting feed additive for pregnant sows, its preparation method and application. However, the present invention is not limited to the above-mentioned embodiments, that is, it does not mean that the present invention must rely on the above-mentioned embodiments to be implemented. Those skilled in the art should understand that any improvement to the present invention, the equivalent substitution of each raw material of the products of the present invention, the addition of auxiliary components, the selection of specific methods, etc. all fall within the protection scope and the disclosure scope of the present invention.

[0132] The preferred embodiments of the present invention have been described in detail above. However, the present invention is not limited to the specific details in the above-mentioned embodiments. Within the technical concept of the present invention, various simple modifications can be made to the technical solutions of the present invention, and these simple modifications all belong to the protection scope of the present invention.

[0133] In addition, it should be noted that, in the case of no contradiction, the various specific technical features described in the above-mentioned specific embodiments can be combined in any suitable way. In order to avoid unnecessary repetition, the present invention will not separately describe various possible combination methods.

Claims

1. A feed additive for promoting reproduction of pregnant sows, characterized in that, The components of the pregnant sow reproduction-promoting feed additive include, by weight: 200-500 parts of fermented Chinese herbal medicine compound, 50-200 parts of compound fat powder, 1-10 parts of ergothioneine, 10-25 parts of yeast selenium and 400-800 parts of carrier material; The raw materials for preparing the composite fat powder include 30-50 parts of butyric acid glyceride, 1-10 parts of lipoic acid, 1-10 parts of nicotinamide and 40-70 parts of wall material in parts by mass; The fermented Chinese herbal medicine complex is a product obtained by fermenting a Chinese herbal medicine fermentation substrate with a composite bacteria and a composite enzyme; The composite bacteria is a combination of yeast and Bacillus subtilis; The complex enzyme is a combination of xylanase, cellulase and neutral protease; The Chinese herbal fermentation substrate is a combination of 5-10 parts of sage, 1-5 parts of magnolia bark, 1-10 parts of raspberry, 1-5 parts of roselle and 1-10 parts of quercetin, calculated by weight.

2. The reproductive promoting feed additive for pregnant sows according to claim 1, characterized in that The carrier material includes rice husk powder.

3. The pregnancy sow reproductive promoting feed additive according to claim 1, characterized in that, The wall material comprises maltodextrin and casein.

4. The pregnancy sow reproductive promoting feed additive according to claim 1, wherein The compound fat powder is obtained by mixing various raw materials with water and then spray-drying.

5. The pregnancy sow reproductive promoting feed additive according to claim 1, characterized in that, The ratio of the viable count of the yeast to that of Bacillus subtilis is 1:(1 - 4), and the total viable count of the composite bacteria is greater than 1×10 9 CFU / g.

6. The reproductive promoting feed additive for pregnant sows according to claim 1, wherein The dosage of the composite bacteria is 1-5% of the mass of the Chinese herbal medicine fermentation substrate.

7. The reproductive promoting feed additive for pregnant sows according to claim 1, characterized in that, The mass ratio of xylanase, cellulase and neutral protease is (1-3):(2-4):(2-3).

8. The reproductive promoting feed additive for pregnant sows according to claim 1, characterized in that, The dosage of the complex enzyme is 0.5-2% of the mass of the Chinese herbal medicine fermentation substrate.

9. The reproductive promoting feed additive for pregnant sows according to claim 1, wherein The fermented Chinese herbal medicine compound is prepared by a preparation method comprising the following steps: (1) Mixing the various Chinese herbal fermentation substrates, crushing them, sieving them, and setting them aside; mixing the composite bacteria with 30-40° C. water for oxygen activation treatment, and then mixing them with the composite enzyme for setting aside; (2) mixing the obtained bacterial enzyme liquid with the treated fermentation substrate, performing anaerobic fermentation, and drying to obtain the fermented Chinese herbal medicine complex.

10. The pregnancy sow reproductive promoting feed additive according to claim 9, characterized in that, The duration of the oxygen infusion activation treatment is 3-5 hours.

11. The reproductive promoting feed additive for pregnant sows according to claim 9, characterized in that, The temperature of the anaerobic fermentation is 15-40° C., and the time of the anaerobic fermentation is 3-7 days.

12. The preparation method of the reproductive promoting feed additive for pregnant sows according to any one of claims 1-11, characterized in that, The preparation method comprises: uniformly mixing the fermented Chinese herbal medicine compound, compound fat powder, ergothioneine, yeast selenium and a carrier material.

13. A reproductive-promoting feed for pregnant sows, characterized in that, The gestating sow reproduction promoting feed contains a basic diet and the gestating sow reproduction promoting feed additive described in any one of claims 1 to 11, and the gestating sow reproduction promoting feed additive accounts for 0.1% to 0.5% of the mass of the basic diet.

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