A Chinese medicinal composition for soothing the nerves and promoting sleep from Xanthoceras sorbifolium Bunge and its preparation method

Abstract

The present invention discloses a Xanthoceras sorbifolium Bunge traditional Chinese medicine composition for soothing the nerves and helping sleep in the technical field of traditional Chinese medicine, and a preparation method thereof, comprising the following components in parts by weight: 30-50 parts of microcapsules of Xanthoceras sorbifolium Bunge fruit shell extract, 10-30 parts of wild jujube seed, 5-15 parts of Poria cocos, 10-30 parts of Salvia miltiorrhiza, 6-10 parts of oyster, and 5-10 parts of dragon bone; the microcapsules of Xanthoceras sorbifolium Bunge fruit shell extract are prepared by microwave-assisted enzymatic hydrolysis and purification of Xanthoceras sorbifolium Bunge fruit shell; in order to solve the problem of poor sleep quality of some people after entering the plateau environment, the present invention proposes to purify the Xanthoceras sorbifolium Bunge fruit shell extract and prepare microcapsules, achieving the effectiveness and stability of the extract, improving the human body's hypoxia tolerance ability after use, and further achieving the technical effect of soothing the nerves and helping sleep; at the same time, a variety of traditional Chinese medicines are used in combination with the microcapsules to prepare the composition, further strengthening the technical effect of soothing the nerves and helping sleep.

Description

Technical Field

[0001] The present invention belongs to the technical field of traditional Chinese medicine, and specifically refers to a Chinese medicine composition for soothing the nerves and promoting sleep made from Xanthoceras sorbifolium Bunge and its preparation method. Background Art

[0002] Insomnia is a relatively common neurological disease. Long-term low-quality sleep can reduce people's cognitive ability, cause negative emotions, and damage the nervous system; people living at low altitudes often experience some altitude sickness when entering high-altitude areas, and insomnia is one of them. The hypoxic environment at high altitudes can change the human sleep structure, increasing the number of fragmented sleep, apnea, and hypopnea, resulting in a decline in sleep quality; Xanthoceras sorbifolium Bunge has high nutritional value and can be used as a vegetable. Its fruit shell contains various saponins, which can improve the body's hypoxia tolerance ability, but due to the difficulty of extraction and preservation, it is rarely used in actual life; Whey protein is initially decomposed in gastric juice and digested and absorbed in the intestine. Puerarin can promote the gelation of whey protein, make the protein network finer and more uniform, and enhance the emulsifying property of the protein; Sleeping pills mainly for sedation and hypnosis are prone to cause central nervous and kidney function disorders after long-term use;

[0003] Currently, the existing technologies mainly have the following problems: First, long-term use of sleeping pills is prone to cause central nervous and kidney function disorders; Second, it is impossible to solve the impact of the high-altitude hypoxic environment on the human body; Third, the effective components of traditional Chinese medicine preparations are difficult to extract, are easily decomposed, and are easily destroyed by gastric acid after being taken. Summary of the Invention

[0004] In view of the above situation, in order to overcome the defects of the existing technologies, the present invention provides a Chinese medicine composition for soothing the nerves and promoting sleep made from Xanthoceras sorbifolium Bunge and its preparation method. In order to solve the problem of poor sleep quality of some people after entering the plateau environment, the present invention proposes to purify the extract of Xanthoceras sorbifolium Bunge fruit shell and prepare microcapsules, realizing the effectiveness and stability of the extract, improving the body's hypoxia tolerance ability after use, and thus achieving the technical effect of soothing the nerves and promoting sleep; at the same time, a variety of traditional Chinese medicines are used to mix with the microcapsules to prepare the composition, further strengthening the technical effect of soothing the nerves and promoting sleep.

[0005] In order to achieve the above object, the technical solution adopted by the present invention is as follows: The present invention proposes a Chinese medicine composition for soothing the nerves and promoting sleep made from Xanthoceras sorbifolium Bunge, and the Chinese medicine composition includes the following components in parts by weight: 30-50 parts of microcapsules of Xanthoceras sorbifolium Bunge fruit shell extract, 10-30 parts of Ziziphus jujuba var. spinosa, 5-15 parts of Poria cocos, 10-30 parts of Salvia miltiorrhiza, 6-10 parts of Oyster, and 5-10 parts of Dragon Bone.

[0006] Preferably, the preparation method of the microcapsules of Xanthoceras sorbifolium Bunge fruit shell extract specifically includes the following steps:

[0007] S1. Screen, wash, dry, cut, freeze-dry, pulverize the shell of Xanthoceras sorbifolium Bunge, and pass through a 60-mesh sieve to obtain shell powder;

[0008] S2. Add the shell powder obtained in S1 into an ethanol solution, stir and mix evenly at a rotation speed of 500 rpm, and perform dynamic microwave treatment to obtain a mixed solution;

[0009] S3. Adjust the pH of the mixed solution obtained in S2 to 4 - 6, add cellulase, stir and mix evenly, perform water bath heat preservation, and centrifuge at a rotation speed of 12000 rpm for 8 min to take the supernatant;

[0010] S4. Under the condition of constant temperature at 45 °C, stir the supernatant obtained in S3 at a rotation speed of 120 r / min for reduced pressure evaporation and concentration to obtain a concentrated solution;

[0011] S5. Weigh the resin, add it into the concentrated solution, adjust the pH to 6, oscillate at a speed of 120 r / min, perform water bath at a constant temperature of 45 °C for 6 h, filter out the resin saturated with adsorption, rinse it clean with distilled water, dry the excess water, add an ethanol solution, oscillate at a speed of 120 r / min, perform water bath at a constant temperature of 45 °C for 6 h, and freeze-dry to obtain an extract;

[0012] S6. Take the extract obtained in S5 and prepare an aqueous solution with a mass concentration of 8% as the core material solution. Take an aqueous solution of whey protein, add puerarin, heat it in a water bath under magnetic stirring, keep it for 15 min, and then cool it to room temperature using an ice water bath to prepare a puerarin - polywhey protein mixed wall material solution. Stir the core material solution and the wall material solution evenly at a rotation speed of 800 r / min to obtain a mixed emulsion;

[0013] S7. Add Tween - 20 as a fixing solution into an aqueous solution of CaCl2 with a concentration of 16.7% (w / v). The volume concentration of Tween - 20 in the fixing solution is 0.04%. The volume ratio of the mixed emulsion obtained in S6 to the fixing solution is 1:10. Use a microcapsule granulator to prepare microcapsules. Parameter settings: nozzle aperture 300 μm, flow rate 6 mL / min, frequency 1300 Hz, voltage 2000 mV, to obtain microcapsules of Xanthoceras sorbifolium Bunge shell extract.

[0014] Preferably, in S2, the volume concentration of the ethanol solution is 65 - 75%, and the addition amount of the shell powder in the ethanol solution is 30 - 40 mg / mL;

[0015] Preferably, in S2, the microwave power is 270 - 320 W, the microwave time is 15 - 25 min, and the microwave temperature is 50 - 70 °C;

[0016] Preferably, in S3, the addition amount of cellulase is 2 - 4% of the mass of the shell powder;

[0017] Preferably, in S3, the water bath temperature is 50 - 65 °C, and the heat preservation time is 1 - 2 h;

[0018] Preferably, in S5, the resin is AB - 8 macroporous adsorption resin, and the addition amount of the resin is 90 - 130 mg / mL;

[0019] Preferably, in S5, the volume concentration of the ethanol solution is 70 - 80%, and the mass - volume ratio of the resin to the ethanol solution is 50 - 80 mg / mL;

[0020] Preferably, in S6, the mass concentration of the whey protein aqueous solution is 9 - 14%, and the addition amount of puerarin is 0.5 mg - 1 mg / mL;

[0021] Preferably, in S6, the water bath heating temperature is 70 - 80 °C, and the volume ratio of the core material solution to the wall material solution is 1:6 - 9.

[0022] The present invention also provides a preparation method of a Xanthoceras sorbifolium Bunge Chinese medicinal composition for calming the nerves and promoting sleep, which specifically includes the following steps: After decocting oyster, dragon bone for 20 min, mix and decoct with wild jujube seed, poria cocos, and salvia miltiorrhiza for 50 min, filter, and add the Xanthoceras sorbifolium Bunge fruit shell extract microcapsule after freeze - drying and mix evenly to obtain the Xanthoceras sorbifolium Bunge Chinese medicinal composition for calming the nerves and promoting sleep.

[0023] The beneficial effects obtained by the present invention are as follows:

[0024] In the present invention, by preparing the Xanthoceras sorbifolium Bunge fruit shell extract in the form of microcapsules with various traditional Chinese medicine components into a composition, the stability of the active ingredients in the Xanthoceras sorbifolium Bunge fruit shell extract is enhanced, the action of the composition is mild, and the effect of calming the nerves and promoting sleep in the high - altitude and low - oxygen environment is improved; the saponin components in the Xanthoceras sorbifolium Bunge fruit shell can improve the hypoxia tolerance of blood, brain, and myocardium, the saponins, flavonoids, and alkaloids in wild jujube seed have the effects of sedation and hypnosis, the polysaccharides in poria cocos have the effect of helping sleep, salvia miltiorrhiza has the effect of clearing the heart and relieving restlessness, and oyster combined with dragon bone can play a role in calming the nerves; adding puerarin to the microcapsule wall material can promote the gelation of whey protein, make the whey protein network finer and more uniform, and enhance the emulsifying property of whey protein; by adopting the method of dynamic microwave enzymatic hydrolysis extraction of the Xanthoceras sorbifolium Bunge fruit shell, the release of active ingredients is promoted, the extraction rate of the extract is increased, and the extraction speed of active ingredients is accelerated; by preparing poly - whey protein microcapsules, the stability of active ingredients is improved, the storage time is prolonged, the destruction of active ingredients by gastric juice after taking is reduced, and more active ingredients are absorbed by the human body; by preparing the composition, the active ingredients contained in various traditional Chinese medicines are gathered, and the effect of calming the nerves and promoting sleep is improved. Description of the Drawings

[0025] Figure 1 It is a result graph of the test on the influence of the environment on the preservation rate of the extract in Examples 1 - 4 and Comparative Example 1 of the present invention;

[0026] Figure 2 This is the result graph of the test on the influence of gastric juice on the preservation rate of the extracts in Examples 1-4 and Comparative Example 1 of the present invention;

[0027] Figure 3 This is the result graph of the influence of the compositions in Example 1, Comparative Example 1, and Comparative Example 2 of the present invention on the survival time of mice in the hypoxia experiment;

[0028] Figure 4 This is the result graph of the influence of taking the compositions obtained in Example 1, Comparative Examples 1-2 of the present invention on the sleep efficiency of people in the plateau environment.

[0029] The accompanying drawings are used to provide a further understanding of the present invention, and constitute a part of the specification. Together with the embodiments of the present invention, they are used to explain the present invention, and do not constitute a limitation to the present invention. Detailed implementation manners

[0030] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments; based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0031] Unless otherwise defined, all professional and scientific terms used herein have the same meaning as those familiar to those skilled in the art. In addition, any methods and materials similar or equivalent to the described content can be applied to the present invention. The preferred implementation methods and materials described herein are only for illustrative purposes and do not limit the content of this application.

[0032] The experimental methods in the following embodiments are all conventional methods unless otherwise specified; the test materials used in the following embodiments are all obtained from commercial channels unless otherwise specified.

[0033] AB-8 macroporous adsorption resin (CasNo: 9003-69-4), purchased from Beijing Innochem Science & Technology Co., Ltd., product number B602761;

[0034] Whey protein (CasNo: 84082-51-9), purchased from Beijing Innochem Science & Technology Co., Ltd., product number W96141;

[0035] Puerarin (CasNo: 3681-99-0), purchased from Beijing Innochem Science & Technology Co., Ltd., product number GC44011;

[0036] Cellulase (CasNo: 84082-51-9), purchased from Beijing Innochem Science & Technology Co., Ltd., product number W96141.

[0037] Example 1

[0038] A Chinese medicinal composition for soothing the nerves and promoting sleep made from Xanthoceras sorbifolium Bunge. The composition includes the following components in parts by weight: 30 parts of microcapsules of Xanthoceras sorbifolium Bunge fruit shell extract, 10 parts of Semen Ziziphi Spinosae, 5 parts of Poria cocos, 10 parts of Salvia miltiorrhiza, 6 parts of Concha Ostreae, and 5 parts of Os Draconis.

[0039] The preparation method of the microcapsules of Xanthoceras sorbifolium Bunge fruit shell extract specifically includes the following steps:

[0040] S1. Screen, wash, dry, cut, freeze-dry, pulverize the Xanthoceras sorbifolium Bunge fruit shell, and pass through a 60-mesh sieve to obtain fruit shell powder;

[0041] S2. Add the fruit shell powder obtained in S1 into an ethanol solution with a volume concentration of 65%. The addition amount of the fruit shell powder in the ethanol solution is 30 mg / mL. Stir and mix evenly at a rotation speed of 500 rpm, and perform dynamic microwave treatment at a power of 270 W for 15 min at a temperature of 50 °C to obtain a mixed solution;

[0042] S3. Adjust the pH of the mixed solution obtained in S2 to 4, add cellulase, and the addition amount is 2% of the mass of the fruit shell powder in S2. Stir and mix evenly, perform water bath insulation at 50 °C for 1 h, and centrifuge at a rotation speed of 12000 rpm for 8 min to take the supernatant;

[0043] S4. Under the condition of constant temperature at 45 °C, stir the supernatant obtained in S3 at a rotation speed of 120 r / min for reduced pressure evaporation and concentration to obtain a concentrated solution;

[0044] S5. Weigh AB-8 macroporous adsorption resin, add it to the concentrated solution, and the addition amount is 90 mg / mL. Adjust the pH to 6, shake at a speed of 120 r / min, perform water bath at a constant temperature of 45 °C for 6 h, filter out the resin saturated with adsorption, rinse it with distilled water, dry it to remove excess water, add an ethanol solution with a volume concentration of 70%, and the mass-volume ratio of the resin to the ethanol solution is 50 - 80 mg / mL. Shake at a speed of 120 r / min, perform water bath at a constant temperature of 45 °C for 6 h, and freeze-dry to obtain an extract;

[0045] S6. Take the extract obtained in S5 and prepare an aqueous solution with a mass concentration of 8% as the core material solution. Take an aqueous solution of whey protein with a mass concentration of 9% and add puerarin at 0.5 mg / mL. Heat it in a water bath at 70 °C under magnetic stirring. After maintaining for 15 min, cool it to room temperature using an ice-water bath to prepare a puerarin-polywhey protein mixed wall material solution. Stir the core material solution and the wall material solution at a rotation speed of 800 r / min to mix evenly to obtain a mixed emulsion, and the volume ratio of the core material solution to the wall material solution is 1:6;

[0046] S7. Add Tween-20 as the fixing solution to an aqueous solution of CaCl2 with a mass concentration of 16.7%. The volume concentration of Tween-20 in the fixing solution is 0.04%. The volume ratio of the mixed emulsion to the fixing solution is 1:10. Use a microcapsule granulator to prepare microcapsules. Parameter settings: nozzle aperture 300 μm; flow rate 6 mL / min; frequency 1300 Hz; voltage 2000 mV to obtain extract microcapsules.

[0047] The present invention also provides a preparation method of a Xanthoceras sorbifolia Bunge traditional Chinese medicine composition for calming the nerves and promoting sleep, which specifically includes the following steps: Mix oyster, dragon bone after decocting for 20 minutes with wild jujube seed, poria cocos, and salvia miltiorrhiza and decoct for 50 minutes, filter, freeze-dry, and add Xanthoceras sorbifolia Bunge fruit shell extract microcapsules and mix evenly to obtain the Xanthoceras sorbifolia Bunge traditional Chinese medicine composition for calming the nerves and promoting sleep.

[0048] Example 2

[0049] A Xanthoceras sorbifolia Bunge traditional Chinese medicine composition for calming the nerves and promoting sleep, the composition includes the following components in parts by weight: 50 parts of Xanthoceras sorbifolia Bunge fruit shell extract microcapsules, 30 parts of wild jujube seed, 15 parts of poria cocos, 30 parts of salvia miltiorrhiza, 10 parts of oyster, and 10 parts of dragon bone.

[0050] The preparation method of Xanthoceras sorbifolia Bunge fruit shell extract microcapsules specifically includes the following steps:

[0051] S1. Screen, wash, dry, cut, freeze-dry, crush, and pass through a 60-mesh sieve the Xanthoceras sorbifolia Bunge fruit shell to obtain fruit shell powder;

[0052] S2. Add the fruit shell powder obtained in S1 to an ethanol solution with a volume concentration of 75%. The addition amount of the fruit shell powder in the ethanol solution is 40 mg / mL. Stir and mix evenly at a rotation speed of 500 rpm, and perform dynamic microwave treatment at a power of 320 W for 25 minutes at a temperature of 70 °C to obtain a mixed solution;

[0053] S3. Adjust the pH of the mixed solution obtained in S2 to 6, add cellulase, and the addition amount is 4% of the mass of the fruit shell powder in S2. Stir and mix evenly, perform water bath insulation at 65 °C for 2 h, and centrifuge at a rotation speed of 12000 rpm for 8 min to take the supernatant;

[0054] S4. Under the condition of constant temperature at 45 °C, stir the supernatant obtained in S3 at a rotation speed of 120 r / min for vacuum evaporation and concentration to obtain a concentrated solution;

[0055] S5. Weigh AB-8 macroporous adsorption resin and add it to the concentrated solution. The addition amount is 130 mg / mL. Adjust the pH to 6, shake at a speed of 120 r / min, and perform water bath at a constant temperature of 45 °C for 6 h. Filter out the resin with saturated adsorption, wash it clean with distilled water, suck dry the excess water, add ethanol solution with a volume concentration of 80%, and the volume ratio of the resin to the ethanol solution is 1:5. Shake at a speed of 120 r / min and perform water bath at a constant temperature of 45 °C for 6 h. Then freeze-dry to obtain the extract;

[0056] S6. Take the extract obtained in S5 and prepare an aqueous solution with a mass concentration of 8% as the core material solution. Take an aqueous solution of whey protein with a mass concentration of 14% and add puerarin at 1 mg / mL. Heat it in a water bath at 80 °C under magnetic stirring. After maintaining for 15 min, cool it to room temperature using an ice-water bath to prepare a puerarin-polywhey protein mixed wall material solution. Stir the core material solution and the wall material solution at a speed of 800 r / min to mix them evenly to obtain a mixed emulsion, and the volume ratio of the core material solution to the wall material solution is 1:9;

[0057] S7. Add Tween-20 to the aqueous solution of CaCl2 with a mass concentration of 16.7% as the fixing solution. The volume concentration of Tween-20 in the fixing solution is 0.04%. The volume ratio of the mixed emulsion to the fixing solution is 1:10. Use a microcapsule granulator to prepare microcapsules. Parameter settings: nozzle aperture 300 μm; flow rate 6 mL / min; frequency 1300 Hz; voltage 2000 mV to obtain the extract microcapsules.

[0058] The present invention also provides a preparation method of the Xanthoceras sorbifolium Bunge traditional Chinese medicine composition for calming the nerves and promoting sleep, which is the same as that in Example 1.

[0059] Example 3

[0060] A Xanthoceras sorbifolium Bunge traditional Chinese medicine composition for calming the nerves and promoting sleep, the composition comprises the following components in parts by weight: 40 parts of microcapsules of Xanthoceras sorbifolium Bunge fruit shell extract, 20 parts of Ziziphus jujuba var. spinosa, 10 parts of Poria cocos, 15 parts of Salvia miltiorrhiza, 8 parts of Oyster, 8 parts of Dragon's Bone.

[0061] A preparation method of the microcapsules of Xanthoceras sorbifolium Bunge fruit shell extract, specifically comprising the following steps:

[0062] S1. Screen, wash, dry, cut, freeze-dry, crush, and sieve the Xanthoceras sorbifolium Bunge fruit shell through a 60-mesh sieve to obtain fruit shell powder;

[0063] S2. Add the fruit shell powder obtained in S1 to an ethanol solution with a volume concentration of 70%. The addition amount of the fruit shell powder in the ethanol solution is 35 mg / mL. Stir and mix evenly at a rotation speed of 500 rpm, and perform dynamic microwave treatment at a power of 300 W and a temperature of 60 °C for 18 min to obtain a mixed solution;

[0064] S3. Adjust the pH of the mixed solution obtained in S2 to 5, add cellulase, with the addition amount being 2% of the mass of the fruit shell powder in S2, stir and mix evenly, carry out water bath insulation at 55 °C for 1.5 h, and centrifuge at 12,000 rpm for 8 min to obtain the supernatant;

[0065] S4. Under the condition of constant temperature at 45 °C, stir the supernatant obtained in S3 at a speed of 120 r / min for vacuum evaporation and concentration to obtain a concentrated solution;

[0066] S5. Weigh AB-8 macroporous adsorption resin, add it to the concentrated solution, with the addition amount being 115 mg / mL, adjust the pH to 6, shake at a speed of 120 r / min, carry out water bath at 45 °C for 6 h, filter out the resin saturated with adsorption, wash it clean with distilled water, suck dry the excess water, add an ethanol solution with a volume concentration of 80%, with the volume ratio of the resin to the ethanol solution being 1:4, shake at a speed of 120 r / min, carry out water bath at 45 °C for 6 h, and freeze-dry to obtain the extract;

[0067] S6. Take the extract obtained in S5 and prepare an aqueous solution with a mass concentration of 8% as the core material solution. Take an aqueous solution of whey protein with a mass concentration of 12% and add puerarin at 0.7 mg / mL, heat it in a water bath at 80 °C under magnetic stirring, keep it for 15 min and then cool it to room temperature using an ice-water bath to prepare a puerarin-polywhey protein mixed wall material solution. Stir the core material solution and the wall material solution at a speed of 800 r / min to mix them evenly to obtain a mixed emulsion, with the volume ratio of the core material solution to the wall material solution being 1:7;

[0068] S7. Add Tween-20 to an aqueous solution of CaCl2 with a mass concentration of 16.7% as the fixing solution, with the volume concentration of Tween-20 in the fixing solution being 0.04%, and the volume ratio of the mixed emulsion to the fixing solution being 1:10. Use a microcapsule granulator to prepare microcapsules, with parameter settings: nozzle aperture 300 μm; flow rate 6 mL / min; frequency 1300 Hz; voltage 2000 mV to obtain the extract microcapsules.

[0069] The present invention also provides a preparation method of the Xanthoceras sorbifolium Bunge traditional Chinese medicine composition for calming the nerves and promoting sleep, which is the same as that in Example 1.

[0070] Example 4

[0071] A Xanthoceras sorbifolium Bunge traditional Chinese medicine composition for calming the nerves and promoting sleep, the composition comprises the following components in parts by weight: 35 parts of Xanthoceras sorbifolium Bunge fruit shell extract microcapsules, 12 parts of Semen Ziziphi Spinosae, 7 parts of Poria cocos, 13 parts of Salvia miltiorrhiza, 7 parts of Concha Ostreae, 9 parts of Os Draconis.

[0072] A preparation method of the Xanthoceras sorbifolium Bunge fruit shell extract microcapsules specifically comprises the following steps:

[0073] S1. Screen, wash, dry, cut, freeze-dry, pulverize the shell of Xanthoceras sorbifolium Bunge, and pass through a 60-mesh sieve to obtain shell powder;

[0074] S2. Add the shell powder obtained in S1 into an ethanol solution with a volume concentration of 72%. The addition amount of the shell powder in the ethanol solution is 35 mg / mL. Stir and mix evenly at a rotation speed of 500 rpm, and perform dynamic microwave treatment at a power of 290 W for 17 min at a temperature of 60 °C to obtain a mixed solution;

[0075] S3. Adjust the pH of the mixed solution obtained in S2 to 4, add cellulase, and the addition amount is 4% of the mass of the shell powder in S2. Stir and mix evenly, keep it in a water bath at 65 °C for 1 h, and centrifuge at a rotation speed of 12000 rpm for 8 min to take the supernatant;

[0076] S4. Under the condition of constant temperature at 45 °C, stir the supernatant obtained in S3 at a rotation speed of 120 r / min for vacuum evaporation and concentration to obtain a concentrated solution;

[0077] S5. Weigh AB-8 macroporous adsorption resin, add it into the concentrated solution, and the addition amount is 115 mg / mL. Adjust the pH to 6, shake it at a speed of 120 r / min, keep it in a water bath at 45 °C for 6 h, filter out the resin saturated with adsorption, wash it clean with distilled water, dry the excess water, add an ethanol solution with a volume concentration of 73%, and the volume ratio of the resin to the ethanol solution is 1:5. Shake it at a speed of 120 r / min, keep it in a water bath at 45 °C for 6 h, and freeze-dry to obtain an extract;

[0078] S6. Take the extract obtained in S5 and prepare an aqueous solution with a mass concentration of 8% as the core material solution. Take an aqueous solution of whey protein with a mass concentration of 12% and add puerarin at 0.7 mg / mL. Heat it in a water bath at 75 °C under magnetic stirring. After maintaining for 15 min, use an ice-water bath to cool it to room temperature to prepare a puerarin-polywhey protein mixed wall material solution. Stir the core material solution and the wall material solution at a rotation speed of 800 r / min to mix evenly to obtain a mixed emulsion, and the volume ratio of the core material solution to the wall material solution is 1:7;

[0079] S7. Add Tween-20 into an aqueous solution of CaCl2 with a mass concentration of 16.7% as a fixing solution. The volume concentration of Tween-20 in the fixing solution is 0.04%. The volume ratio of the mixed emulsion to the fixing solution is 1:10. Use a microcapsule granulator to prepare microcapsules. Parameter settings: nozzle aperture 300 μm; flow rate 6 mL / min; frequency 1300 Hz; voltage 2000 mV to obtain extract microcapsules.

[0080] The present invention also provides a preparation method of a traditional Chinese medicine composition for soothing the nerves and promoting sleep from Xanthoceras sorbifolium Bunge, which is the same as that in Example 1.

[0081] Comparative Example 1

[0082] This comparative example provides a traditional Chinese medicine composition, which is different from Example 3 only in that puerarin-polywhey protein is not included in the components, and the remaining components and component contents are the same as those in Example 3.

[0083] Comparative Example 2

[0084] This comparative example provides a traditional Chinese medicine composition, which is different from Example 3 only in that the microcapsule of Xanthoceras sorbifolia Bunge fruit shell extract is not included in the components, and the remaining components and component contents are the same as those in Example 3.

[0085] Experimental Example 1

[0086] Take the extract microcapsules obtained in Examples 1-4 and the extract obtained in Comparative Example 1, and perform sunlight irradiation treatment under the conditions of 80% humidity and 60°C temperature, and store them for 0d, 5d, 10d, 15d, 20d, and 25d respectively. Measure the saponin content. Taking the content at 0d as the initial content, calculate the preservation rate of saponin according to the following formula:

[0087] Preservation rate = total saponin content / initial content × 100%.

[0088] Experimental Example 2

[0089] Dissolve 1.5 g of pepsin in 500 ml of 0.5% (w / v) NaCl solution, adjust the pH to 2.0 to obtain simulated gastric juice. Take the Xanthoceras sorbifolia Bunge fruit shell extract microcapsules obtained in Examples 1-4 and the Xanthoceras sorbifolia Bunge fruit shell extract obtained in Comparative Example 1 and add them to the artificial gastric juice respectively. According to the gastric emptying time of 4 h, shake at a speed of 100 rpm, and under the condition of 37°C water bath, store for 0 h, 1 h, 2 h, 3 h, and 4 h respectively. Measure the saponin content in the simulated gastric juice. Taking the content at 0 h as the initial content, calculate the preservation rate according to the formula in Experimental Example 1.

[0090] Experimental Example 3 Mouse Simulated Plateau Experiment

[0091] Four groups of mice, 6 in each group, were raised. The control group was fed with conventional feed. In the experimental groups, the compositions obtained in Example 1 and Comparative Examples 1-2 were uniformly mixed into the feed at an addition amount of 2%. After three weeks of feeding, the mice were placed in a 250 mL wide-mouth bottle containing 5 g of soda lime and sealed for an anoxic experiment under normal pressure. Record the anoxic survival time of each group of mice;

[0092] Experimental Example 4 Human Simulated Plateau Experiment

[0093] A method of simulating the plateau environment by reducing the oxygen concentration was adopted. 120 volunteers with good sleep conditions were selected and divided into 4 groups for sleep tests. The subjects were required not to consume coffee, alcohol or smoke, and the temperature in the hypoxic chamber was kept constant at (25±1)°C. The first week was for sleep tests in a plain environment, the second week was for sleep tests in a normal pressure environment with an oxygen concentration of 16%, the third week was for sleep tests in a normal low pressure environment with an oxygen concentration of 14%, and the fourth week was for sleep tests in a normal pressure environment with an oxygen concentration of 12%. Each group had 30 people. The experimental groups were respectively given the compositions obtained in Example 1 and Comparative Examples 1-2, and the control group was given an equal amount of whey protein as a placebo. During the sleep test, a polysomnograph was used to monitor the sleep process and record the sleep efficiency.

[0094] Result analysis

[0095] Figure 1 This is a graph showing the test results of the influence of the environment on the preservation rate of the extracts in Examples 1-4 of the present invention and Comparative Example 1. As shown in the figure, the saponin preservation rates of Examples 1-4 were 74.65%, 74.85%, 76.94%, and 72.97% respectively, and the saponin preservation rate of Comparative Example 1 was 53.32%. The saponin preservation rates in Examples 1-4 were significantly higher than those in Example 1, indicating that the use of puerarin - polywhey protein microcapsule coating can effectively improve the stability of the active ingredients of the extract during preservation.

[0096] Figure 2 This is a graph showing the test results of the influence of gastric juice on the preservation rate of the extracts in Examples 1-4 of the present invention and Comparative Example 1. As shown in the figure, the saponin content preservation rates of Examples 1-4 were 80.62%, 81.16%, 83.59%, and 76.82% respectively, and the saponin content preservation rate of Comparative Example 1 was 40.37%. The saponin preservation rates in Examples 1-4 were significantly higher than those in Comparative Example 1, indicating that the use of puerarin - polywhey protein microcapsule coating can effectively reduce the damage of gastric juice to the active ingredients, increase the proportion of the active ingredients reaching the small intestine for absorption. Whey protein lacks specific sites for hydrolysis by pepsin and is not easily decomposed in gastric juice. Puerarin enhances the binding stability of whey protein, making the microcapsule more difficult to be decomposed by gastric juice, while trypsin in the intestine can well decompose whey protein, enabling the active ingredients to be released and absorbed in the intestine.

[0097] Figure 3This is the result graph of the influence of the compositions in Example 1 and Comparative Examples 1-2 of the present invention on the survival time of mice in the hypoxia experiment. As shown in the figure, the survival times of Example 1, Comparative Examples 1-2, and the control group are 1216.53 s, 1056.86 s, 973.98 s, and 961.21 s respectively; in Example 1 and Comparative Example 1, the survival times are significantly higher than those of the control group and Comparative Example 2, indicating that the extract of Xanthoceras sorbifolia Bunge fruit shell can enhance the hypoxia tolerance ability of mice. The saponins in the extract can maintain the ATP level of cardiomyocytes within the normal range during hypoxia, make the secretion of neurotransmitters close to normal, and reduce the damage caused by hypoxia to cells; the survival time in Example 1 is significantly higher than that in Comparative Example 1, indicating that using puerarin-polywhey protein microcapsules to protect the active ingredients can reduce the decomposition of the active ingredients by gastric juice, and thus enhance the absorption of the active ingredients in mice.

[0098] Figure 4 This is the result graph of the influence of taking the compositions obtained in Example 1 and Comparative Examples 1-2 of the present invention on the sleep efficiency of people in the plateau environment. As shown in the figure, in the fourth week, the sleep efficiencies of Example 1, Comparative Examples 1-2, and the control group are 86.65%, 79.23%, 78.86%, and 74.49% respectively; by the fourth week, only the sleep efficiency of Example 1 is still above 80%, indicating that the sleep condition of Example 1 is still good; the sleep efficiency of Example 1 is higher than that of the control group, indicating that the composition has a good improvement effect on the sleep disorder caused by the plateau hypoxia environment; the sleep efficiency of Example 1 is higher than that of Comparative Example 1, indicating that using puerarin-polywhey protein microcapsules to protect the active ingredients can reduce the loss in gastric juice, better play the effect of the active ingredients of the Xanthoceras sorbifolia Bunge fruit shell extract on the human body, and improve the sleep efficiency; the sleep efficiency of Example 2 is higher than that of Comparative Example 1, indicating that adding the Xanthoceras sorbifolia Bunge fruit shell extract can improve the hypoxia tolerance ability of the human body, reduce the influence of plateau hypoxia on the human body, and improve the sleep disorder caused by the plateau hypoxia environment to achieve the effect of calming the nerves and helping sleep.

[0099] Although the embodiments of the present invention have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principles and spirits of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

[0100] The above describes the present invention and its implementation manners. This description is not restrictive. What is shown in the drawings is only one of the implementation manners of the present invention, and the actual application is not limited to this. Generally speaking, if those of ordinary skill in the art are inspired by it and, without departing from the purpose of the present invention, design similar ways and embodiments to this technical solution without creative efforts, they should all fall within the protection scope of the present invention.

Claims

1. A traditional Chinese medicine composition for soothing the nerves and promoting sleep from Xanthoceras sorbifolium Bunge, characterized in that: The traditional Chinese medicine composition is made from the following components in parts by weight: 30-50 parts of microcapsules of Xanthoceras sorbifolia Bunge fruit shell extract, 10-30 parts of Semen Ziziphi Spinosae, 5-15 parts of Poria, 10-30 parts of Salvia miltiorrhiza, 6-10 parts of Oyster, and 5-10 parts of Fossilia Ossis Mastodi; The preparation method of the microcapsules of Xanthoceras sorbifolia Bunge fruit shell extract specifically includes the following steps: S1. Screen, wash, dry, cut, freeze-dry, crush, and sieve the Xanthoceras sorbifolia Bunge fruit shell through a 60-mesh sieve to obtain fruit shell powder; S2. Add the fruit shell powder obtained in S1 into an ethanol solution, stir and mix evenly at a rotation speed of 500 rpm, and perform dynamic microwave treatment to obtain a mixed solution; S3. Adjust the pH of the mixed solution obtained in S2 to 4-6, add cellulase, stir and mix evenly, perform water bath insulation, and centrifuge at a rotation speed of 12000 rpm for 8 min to take the supernatant; S4. Under the condition of constant temperature at 45°C, stir the supernatant obtained in S3 at a rotation speed of 120 r / min for vacuum evaporation and concentration to obtain a concentrated solution; S5. Weigh the resin, add it into the concentrated solution, adjust the pH to 6, shake at a speed of 120 r / min, perform water bath at a constant temperature of 45°C for 6 h, filter out the resin saturated with adsorption, rinse it clean with distilled water, dry off the excess water, add an ethanol solution, shake at a speed of 120 r / min, perform water bath at a constant temperature of 45°C for 6 h, and freeze-dry to obtain an extract; S6. Take the extract obtained in S5 and prepare an aqueous solution with a mass concentration of 8% as the core material solution. Take an aqueous solution of whey protein, add puerarin, heat it in a water bath under magnetic stirring, keep it for 15 min, and then cool it to room temperature using an ice-water bath to prepare a puerarin-polywhey protein mixed wall material solution. Stir the core material solution and the wall material solution evenly at a rotation speed of 800 r / min to obtain a mixed emulsion; S7. Add Tween-20 to an aqueous solution of CaCl2 with a mass concentration of 16.7% as a fixing solution. The volume concentration of Tween-20 in the fixing solution is 0.04%. The volume ratio of the mixed emulsion obtained in S6 to the fixing solution is 1:

10. Use a microcapsule granulator to prepare microcapsules. Parameter settings: nozzle aperture 300 um, flow rate 6 mL / min, frequency 1300 Hz, voltage 2000 mV, to obtain microcapsules of Xanthoceras sorbifolia Bunge fruit shell extract; In S2, the volume concentration of the ethanol solution is 65-75%, and the addition amount of the fruit shell powder in the ethanol solution is 30-40 mg / mL; In S3, the addition amount of cellulase is 2-4% of the mass of the fruit shell powder; the water bath temperature is 50-65°C, and the heat preservation time is 1-2 h; In S5, the resin is AB-8 macroporous adsorption resin, and the addition amount of the resin is 90-130 mg / mL; In S5, the volume concentration of the ethanol solution is 70-80%, and the mass-volume ratio of the resin to the ethanol solution is 50-80 mg / mL; In S6, the mass concentration of the aqueous solution of whey protein is 9-14%; the addition amount of puerarin is 0.5 mg-1 mg / mL; In S6, the water bath heating temperature is 70-80°C; the volume ratio of the core material solution to the wall material solution is 1:6-9.

2. The Xanthoceras sorbifolium Bunge traditional Chinese medicine composition for soothing the nerves and helping sleep according to claim 1, wherein: In S2, the microwave power is 270 - 320 W, the microwave time is 15 - 25 min, and the microwave temperature is 50 - 70 °C.

3. The preparation method of the Xanthoceras sorbifolia Bunge traditional Chinese medicine composition for calming the nerves and helping sleep according to any one of claims 1-2, characterized in that: Specifically, it includes the following steps: Mix the oysters, keel, jujube seeds, poria cocos, and salvia miltiorrhiza that have been fried for 20 minutes, decoct them for 50 minutes, filter, freeze-dry, and then mix evenly with the xanthoceras sorbifolia bunge fruit shell extract microcapsules to obtain the xanthoceras sorbifolia bunge traditional Chinese medicine composition for tranquilizing the mind and helping sleep.

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