A kind of strengthening and strengthening ointment and preparation method thereof

Abstract

The invention discloses a strengthening and strengthening paste. The strengthening and strengthening paste is prepared from the following raw materials in parts by weight: 10-18 parts of aconite, 12-20 parts of cinnamon bark, 4-8 parts of asarum, 20-28 parts of rehmannia root, 15-25 parts of donkey-hide gelatin, 16-20 parts of ginseng, 24-28 parts of astragalus, 18-22 parts of atractylodes, 15-25 parts of tuckahoe, 12-16 parts of gypsum, 15-25 parts of superbus, 8-12 parts of fennel, 12-16 parts of agarwood and 8-12 parts of lindera striata. The paste has the advantages of warming and nourishing kidney yang, nourishing spleen and replenishing qi, and having remarkable effects of treating kidney qi deficiency and kidney yang deficiency. The invention also discloses a preparation method thereof.

Description

Technical Field

[0001] The present invention relates to a Chinese medicine composition and a preparation method and application thereof, and in particular to a tonic paste and a preparation method and application thereof. The present invention belongs to the technical field of Chinese medicine research. Background Art

[0002] Xu Lao is the name of the diagnosis in Traditional Chinese Medicine, also known as various types of Xu Lao injury, which means that different organs of the human body are in a weak state, and the series of clinical symptoms manifested are usually a chronic process, that is, it occurs and progresses gradually.

[0003] Traditional Chinese medicine believes that the occurrence of asthenia is closely related to the heart, lungs, spleen, and kidneys. In general, it can be divided into qi deficiency, blood deficiency, yin deficiency, and yang deficiency. Then, depending on the different parts of the deficiency, that is, the different organs involved, it can be divided into heart qi deficiency, kidney qi deficiency, spleen qi deficiency, lung qi deficiency and other problems.

[0004] Asthenia can be diagnosed as a separate disease, that is, even if the patient has no other diseases, asthenia can occur. Similar to the chronic fatigue syndrome or sub-health status currently diagnosed by Western medicine, asthenia can also be caused by other diseases, such as severe heart disease, lung dysfunction, or tumor development to a certain extent, which can cause the patient's weakness and can also be diagnosed as asthenia. At present, the most common clinical manifestations are kidney qi deficiency and kidney yang deficiency, which are both types of kidney deficiency. Kidney qi deficiency refers to insufficient kidney qi transformation, and symptoms such as soreness and weakness in the waist and legs, fatigue and weakness, spermatorrhea, urination difficulties, or clear and long urination appear. Kidney yang deficiency refers to insufficient yang qi in the kidney. It is generally believed that kidney yang deficiency is a manifestation of obvious deficiency and cold on the basis of kidney qi deficiency, which is a further development of kidney qi deficiency. Clinically, symptoms such as fear of cold, lower abdominal cramps, and cold semen can be seen. Summary of the invention

[0005] The technical problem to be solved by the present invention is to provide a cream for strengthening the body and strengthening the foundation, and a preparation method and use thereof.

[0006] In order to achieve the above object, the present invention adopts the following technical means:

[0007] The invention discloses a strengthening and strengthening paste, which is prepared from the following raw materials in parts by weight: 10-18 parts of aconite, 12-20 parts of cinnamon bark, 4-8 parts of asarum, 20-28 parts of rehmannia root, 15-25 parts of donkey-hide gelatin, 16-20 parts of ginseng, 24-28 parts of astragalus, 18-22 parts of atractylodes, 15-25 parts of tuckahoe, 12-16 parts of pyrrosia, 15-25 parts of superbus, 8-12 parts of fennel, 12-16 parts of agarwood and 8-12 parts of lindera.

[0008] The described Fuzheng Guben ointment is made of the following raw materials in parts by weight: 14 parts of aconite, 16 parts of cinnamon, 6 parts of asarum, 24 parts of rehmannia root, 20 parts of donkey-hide gelatin, 18 parts of ginseng, 26 parts of astragalus, 20 parts of atractylodes, 20 parts of poria, 14 parts of pyrrosia, 20 parts of superbus, 10 parts of fennel, 14 parts of agarwood, and 10 parts of lindera.

[0009] The described Fuzheng Guben ointment is made of the following raw materials in parts by weight: 10 parts of aconite, 20 parts of cinnamon, 4 parts of asarum, 28 parts of rehmannia root, 15 parts of donkey-hide gelatin, 20 parts of ginseng, 24 parts of astragalus, 22 parts of atractylodes, 15 parts of poria, 16 parts of pyrrosia, 15 parts of superbus, 12 parts of fennel, 12 parts of agarwood, and 12 parts of lindera.

[0010] The described Fuzheng Guben ointment is made of the following raw materials in parts by weight: 18 parts of aconite, 12 parts of cinnamon, 8 parts of asarum, 20 parts of rehmannia root, 25 parts of donkey-hide gelatin, 16 parts of ginseng, 28 parts of astragalus, 18 parts of atractylodes, 25 parts of poria, 12 parts of pyrrosia, 25 parts of superbus, 8 parts of fennel, 16 parts of agarwood, and 8 parts of lindera.

[0011] The described Fuzheng Guben ointment is indicated for kidney qi deficiency and kidney yang deficiency.

[0012] The Fuzheng Guben ointment comprises processed aconite.

[0013] The application of the Fuzheng Guben ointment in the preparation of medicines for improving immunity.

[0014] The preparation method of the Fuzheng Guben ointment comprises the following steps:

[0015] (1) Weigh each raw material drug, mix them evenly, add 20-30 times the weight of the drug in water, soak for 2.5-3.5 hours, decoct for 2.5-3.5 hours, and cool;

[0016] (2) filtering the cooled liquid medicine in step (1) through gauze to obtain a first filtrate;

[0017] (3) Repeating steps (1) and (2) with the remaining drug residue in step (2) to obtain a second filtrate;

[0018] (4) The first filtrate and the second filtrate are combined, concentrated, and refined honey is added to prepare a paste.

[0019] In the described Fuzheng Guben ointment, the filtering with gauze in step (2) refers to filtering with 3 layers of gauze, 5 layers of gauze, 8 layers of gauze, and 10 layers of gauze in sequence.

[0020] Kidney Qi deficiency and kidney Yang deficiency, symptoms: fear of cold and cold limbs, pale or pale complexion, laziness and fatigue, lack of energy, dizziness and tinnitus, cold pain in the waist and spine, diarrhea at dawn, loose stools, spermatorrhea and impotence, or enuresis, or swelling of the whole body, no sensation when pressed, difficulty urinating, or shortness of breath, palpitations and restlessness. Pale or plump tongue, white and slippery tongue coating, slow or slow pulse. Pathogenesis analysis: The kidney is responsible for consolidating storage and controlling qi transformation. The yang in the kidney, namely the primordial yang, is the source of vitality for the human body. The kidney is the gate of the stomach. If the kidney yang qi is deficient, the fire does not generate earth, and the gate is not closed, there will be diarrhea at dawn, diarrhea, incontinence or polyuria; if the gate of semen is not closed and cannot be stored, it will cause spermatorrhea; if the yang cannot warm and invigorate, there will be aversion to cold, cold limbs, pale face, impotence, laziness, fatigue, and lack of energy; the waist is the home of the kidney, and if the kidney yang is insufficient, there will be no warming of the governor vessel, so there will be cold pain in the waist and spine; if the yang is deficient, the body fluid cannot be evaporated, and the qi transformation is weak, there will be edema and oliguria, which is the yang deficiency edema. If the kidney does not receive qi, water qi will rise, and palpitations, wheezing, blue face and purple lips and other water qi rising heart symptoms will be seen, and the tongue will be tender and plump with white and slippery fur. Dizziness and tinnitus are all caused by the inability of yang qi to warm up; the tongue is pale and the pulse is slow, which is the sign of yang deficiency and cold. The present invention treats kidney qi deficiency and kidney yang deficiency through five aspects: first, warming and nourishing kidney yang and liver yang; second, replenishing essence and blood; third, warming and nourishing kidney qi; fourth, nourishing spleen and replenishing qi, strengthening spleen and replenishing deficiency, transporting and transforming food and body fluid; fifth, promoting qi circulation and smoothing qi.

[0021] Kidney yang deficiency is closely related to liver yang deficiency. The present invention uses aconite and cinnamon bark to warm and nourish kidney yang, and then uses asarum to warm and nourish liver yang, which are all monarch drugs. In addition, because "those with insufficient essence should be supplemented with flavor", cooked rehmannia, donkey-hide gelatin and other thick-flavored products are used to replenish essence and blood, and "those with insufficient form should be warmed with qi", ginseng and astragalus are used to warm and nourish kidney qi, and cooked rehmannia, donkey-hide gelatin, ginseng and astragalus are all ministerial drugs; the mother is supplemented for the deficiency, and the child is drained for the excess. "Atractylodes macrocephala and Poria cocos" are used to nourish the spleen and replenish qi, strengthen the spleen and replenish deficiency, transport and transform food and body fluid, pyrrosia and superbus warm the kidney and promote water excretion, and urinate, and cinnamon bark is used to warm yang, transform qi and promote water excretion. Atractylodes macrocephala, Poria cocos, pyrrosia and superbus are adjuvants. Because yang deficiency generates cold, and cold stagnation leads to qi and blood stagnation, fennel, agarwood and lindera strychnos acuminata are used as guiding drugs to promote qi and smooth qi. The whole prescription has remarkable effect in treating kidney qi deficiency and kidney yang deficiency. DETAILED DESCRIPTION

[0022] Embodiment 1:

[0023] Take 14g of aconite root, 16g of cinnamon bark, 6g of asarum, 24g of Rehmannia root, 20g of donkey-hide gelatin, 18g of ginseng, 26g of astragalus, 20g of atractylodes, 20g of Poria, 14g of pyrrosia, 20g of superbus, 10g of fennel, 14g of agarwood, and 10g of lindera striata, and the preparation method comprises the following steps:

[0024] (1) Weigh each raw material drug, mix them evenly, add 20 times the weight of the drug in water, soak for 3.5 hours, boil for 2.5 hours, and cool;

[0025] (2) filtering the cooled liquid medicine in step (1) through gauze, and filtering through 3 layers of gauze, 5 layers of gauze, 8 layers of gauze, and 10 layers of gauze in sequence to obtain a first filtrate;

[0026] (3) Repeating steps (1) and (2) with the remaining drug residue in step (2) to obtain a second filtrate;

[0027] (4) The first filtrate and the second filtrate are combined, concentrated, and refined honey is added to prepare a paste.

[0028] Embodiment 2:

[0029] Take 10g of aconite root, 20g of cinnamon bark, 4g of asarum, 28g of Rehmannia root, 15g of donkey-hide gelatin, 20g of ginseng, 24g of astragalus, 22g of atractylodes, 15g of Poria, 16g of pyrrosia, 15g of Dianthus superbus, 12g of fennel, 12g of agarwood, and 12g of lindera stolonifera. The preparation method comprises the following steps:

[0030] (1) Weigh each raw material drug, mix them evenly, add 25 times the weight of the drug in water, soak for 3 hours, decoct for 3 hours, and cool;

[0031] (2) filtering the cooled liquid medicine in step (1) through gauze, sequentially filtering through 3 layers of gauze, 5 layers of gauze, 8 layers of gauze, and 10 layers of gauze to obtain a first filtrate;

[0032] (3) Repeating steps (1) and (2) with the remaining drug residue in step (2) to obtain a second filtrate;

[0033] (4) The first filtrate and the second filtrate are combined, concentrated, and refined honey is added to prepare a paste.

[0034] Embodiment 3:

[0035] Take 18g of aconite root, 12g of cinnamon bark, 8g of asarum, 20g of Rehmannia root, 25g of donkey-hide gelatin, 16g of ginseng, 28g of astragalus, 18g of atractylodes, 25g of Poria, 12g of pyrrosia, 25g of superbus, 8g of fennel, 16g of agarwood, and 8g of lindera stolonifera, and the preparation method comprises the following steps:

[0036] (1) Weigh each raw material drug, mix them evenly, add 30 times the weight of the drug in water, soak for 2.5 hours, decoct for 3.5 hours, and cool;

[0037] (2) filtering the cooled liquid medicine in step (1) through gauze, sequentially filtering through 3 layers of gauze, 5 layers of gauze, 8 layers of gauze, and 10 layers of gauze to obtain a first filtrate;

[0038] (3) Repeating steps (1) and (2) with the remaining drug residue in step (2) to obtain a second filtrate;

[0039] (4) The first filtrate and the second filtrate are combined, concentrated, and refined honey is added to prepare a paste.

[0040] Example 4

[0041] 1. Materials and Methods

[0042] 1.1 Sample: The following animal experiments were carried out using the Fuzheng Guben paste prepared according to Example 1 (hereinafter referred to as the medicine of the present invention, 14g of Aconite, 16g of Cinnamon, 6g of Asarum, 24g of Rehmannia Glutinosa, 20g of Donkey-hide Gelatin, 18g of Ginseng, 26g of Astragalus, 20g of Atractylodes, 20g of Poria, 14g of Pyrola, 20g of Dianthus Superbus, 10g of Fennel, 14g of Aquilaria, 10g of Linderae, a total of 232g prepared into 50g of paste)

[0043] 1.2 Experimental animals

[0044] 60 healthy male mice, weighing 18g-22g, were selected and divided into 5 groups, with 12 mice in each group, for a delayed-type hypersensitivity experiment; 60 healthy male mice, weighing 18g-22g, were selected and divided into 5 groups, with 12 mice in each group, for a mouse peritoneal macrophage phagocytosis of chicken red blood cells experiment.

[0045] 1.3 Dose selection and preparation

[0046] The daily intake was 6g of the original medicinal material / 60kg BW, equivalent to 0.1g / kg BW. The intragastric dose was set at 1, 10, and 30 times the recommended intake, i.e., 0.1, 1, and 3g / kg BW, and each dose group was diluted with pure water. The positive control group was given Yougui Pills (240g of Rehmannia glutinosa, 60g of Radix Aconiti Lateralis Preparata, 60g of Cinnamon Bark, 120g of Dioscorea opposita, 90g of Cornus officinalis, 120g of Cuscuta chinensis, 120g of Deer Horn Glue, 120g of Lycium barbarum, 90g of Angelica sinensis, and 120g of Eucommia ulmoides. Preparation method: The above ten ingredients, except Deer Horn Glue, are crushed into fine powder, sieved, and mixed. Deer Horn Glue is stewed with white wine. For every 100g of powder, 60-80g of refined honey is added to the stewed Deer Horn Glue to make small or large honey pills, which are obtained), diluted with pure water, and the dose used was 1g / kg BW. Pure water was used as the negative control group, and the intragastric volume of mice in each group was 0.2 mL / 10 g BW. After continuous intragastric administration for 30 days, various immune indicators were measured.

[0047] 1.4 Experimental methods

[0048] Delayed-type hypersensitivity reaction (plantar thickening method DTH): sheep blood was collected and washed with saline. Mice were immunized intraperitoneally with 2% (V / V) SRBC, and 0.2 mL / mouse was injected per mouse. Four days after immunization, the thickness of the left hind paw was measured. 20% (V / V) SRBC was injected subcutaneously at the measurement site, 20 μL / mouse. The thickness of the left hind paw was measured three times 24 hours after injection, and the average value was calculated.

[0049] 1.5 Statistics

[0050] SPSS11.5 statistical software was used for one-way analysis of variance to compare the means. When the variances were equal, the LSD method was used for pairwise comparisons between the groups; when the variances were unequal, the Tamhane method was used for pairwise comparisons between the groups.

[0051] 2 Results

[0052] 2.1 Effects of the test substances on the cellular immune function of mice

[0053] Effects of the test substances on delayed-type hypersensitivity (DTH) in mice

[0054] Table 1 Effects of the test substances on delayed-type hypersensitivity (DTH) in mice

[0055]

[0056]

[0057] P value: Comparison between each experimental group and negative control group *P<0.05 **P<0.01

[0058] As can be seen from Table 1, after statistical processing, after oral administration of the test substance for 30 days, there was no significant difference in the degree of plantar swelling between the low-dose group and the negative control group (P>0.05), and there was a significant difference between the medium and high-dose groups and the negative control group (P<0.05), that is, the medium and high doses of the test substance can enhance the delayed-type hypersensitivity reaction of mice.

[0059] 2.2 Effects of the test substances on the phagocytic function of mouse monocytes and macrophages

[0060] 2.2.1 Effects of the test substances on carbon clearance function of mouse monocytes and macrophages

[0061] Table 2 Effects of the test substances on carbon clearance function of mouse monocyte-macrophages

[0062] Group Phagocytic index (a) Negative control group 6.13±0.97 Low dose group 6.76±1.32 Medium dose group 7.09±1.54* High dose group 7.28±1.67* Positive drug control group 6.34±0.85

[0063] P value: Comparison between each experimental group and negative control group *P<0.05

[0064] As can be seen from Table 2, after oral administration of different doses of the test substance to mice for 30 days, after statistical processing, there was no significant difference in carbon clearance function between the low-dose group and the negative control group (P>0.05), while there was a significant difference between the medium and high-dose groups and the negative control group (P<0.05), that is, the test substance in the medium and high-dose groups can improve the carbon clearance ability of mouse monocytes-macrophages.

[0065] 3. Summary

[0066] Oral administration of different doses of the test substance to mice for 30 days can enhance delayed-type hypersensitivity in mice, that is, can enhance cellular immune function; can improve the carbon clearance capacity of monocyte-macrophages in mice, that is, can enhance monocyte-macrophage function. Therefore, it is determined that Example 3 has the function of enhancing immunity.

[0067] Example 5: Clinical study

[0068] 1. Case diagnosis criteria: patients with kidney qi deficiency and kidney yang deficiency, TCM diagnosis criteria, symptoms: fear of cold and cold limbs, pale or pale complexion, laziness and fatigue, lack of energy, dizziness and tinnitus, cold pain in the waist and spine, diarrhea at dawn, loose stools, spermatorrhea and impotence, or enuresis, or edema of the whole body, no sensation when pressed, difficulty urinating, or shortness of breath, palpitations and restlessness. Pale or plump tongue, white and slippery tongue coating, slow pulse or slow and weak pulse.

[0069] 2. Efficacy standards: formulated with reference to the "Efficacy Standards for Diagnosis of TCM Diseases and Syndromes" of the State Administration of Traditional Chinese Medicine. (1) Cured: symptoms eliminated. (2) Improved: symptoms alleviated. (3) Not cured: symptoms not improved.

[0070] 3. Research method: 100 patients were given the paste prepared in Example 1 of the present invention (daily dosage: aconite 14g, cinnamon 16g, asarum 6g, rehmannia glutinosa 24g, donkey-hide gelatin 20g, ginseng 18g, astragalus 26g, white atractylodes 20g, Poria 20g, pyrrosia 14g, superbus 20g, fennel 10g, agarwood 14g, lindera 10g), and the efficacy was statistically analyzed after 1 month of treatment.

[0071] 4. Results: Among the 100 cases, after taking the Chinese medicine of the present invention, 58 cases were markedly effective, 32 cases were effective, and 10 cases were ineffective, with an effective rate of 90%. No toxic or side effects occurred during the treatment.

[0072] 5. Conclusion: The present invention has good effects of warming and replenishing kidney yang, warming and replenishing liver yang, replenishing essence and blood, warming and replenishing kidney qi, replenishing spleen and replenishing qi, warming kidney and promoting diuresis, warming yang and transforming qi to promote water circulation, promoting qi and smoothing qi, and has significant effects in treating kidney qi deficiency and kidney yang deficiency with few adverse reactions.

[0073] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A cream for strengthening the body, characterized in that: The described reinforcing and strengthening paste is prepared from the following raw materials in parts by weight: 10-18 parts of aconite, 12-20 parts of cinnamon, 4-8 parts of asarum, 20-28 parts of rehmannia root, 15-25 parts of donkey-hide gelatin, 16-20 parts of ginseng, 24-28 parts of astragalus, 18-22 parts of atractylodes, 15-25 parts of poria, 12-16 parts of pyrrosia, 15-25 parts of superbus, 8-12 parts of fennel, 12-16 parts of agarwood, and 8-12 parts of lindera.

2. The strengthening and strengthening ointment as claimed in claim 1, characterized in that: The described Fuzheng Guben ointment is prepared from the following raw materials in parts by weight: 14 parts of aconite, 16 parts of cinnamon, 6 parts of asarum, 24 parts of rehmannia root, 20 parts of donkey-hide gelatin, 18 parts of ginseng, 26 parts of astragalus, 20 parts of atractylodes, 20 parts of poria, 14 parts of pyrrosia, 20 parts of superbus, 10 parts of fennel, 14 parts of agarwood, and 10 parts of lindera.

3. The strengthening and strengthening ointment as claimed in claim 1, characterized in that: The described Fuzheng Guben paste is prepared from the following raw materials in parts by weight: 10 parts of aconite, 20 parts of cinnamon, 4 parts of asarum, 28 parts of rehmannia root, 15 parts of donkey-hide gelatin, 20 parts of ginseng, 24 parts of astragalus, 22 parts of atractylodes, 15 parts of poria, 16 parts of pyrrosia, 15 parts of superbus, 12 parts of fennel, 12 parts of agarwood, and 12 parts of lindera.

4. The strengthening and strengthening ointment as claimed in claim 1, characterized in that: The described Fuzheng Guben paste is prepared from the following raw materials in parts by weight: 18 parts of aconite, 12 parts of cinnamon, 8 parts of asarum, 20 parts of rehmannia root, 25 parts of donkey-hide gelatin, 16 parts of ginseng, 28 parts of astragalus, 18 parts of atractylodes, 25 parts of poria, 12 parts of pyrrosia, 25 parts of superbus, 8 parts of fennel, 16 parts of agarwood, and 8 parts of lindera.

5. The strengthening and strengthening ointment according to claim 1 or 2, characterized in that: Its indications are kidney qi deficiency and kidney yang deficiency.

6. Use of the Fuzheng Guben ointment as claimed in claim 1 or 2 in the preparation of medicines for improving immunity.

7. The strengthening and strengthening ointment according to any one of claims 1 to 4, characterized in that: The preparation method comprises the following steps: (1) Weigh each raw material drug, mix them evenly, add 20-30 times the weight of the drug in water, soak for 2.5-3.5 hours, decoct for 2.5-3.5 hours, and cool; (2) filtering the cooled liquid medicine in step (1) through gauze to obtain a first filtrate; (3) Repeating steps (1) and (2) with the remaining drug residue in step (2) to obtain a second filtrate; (4) The first filtrate and the second filtrate are combined, concentrated, and refined honey is added to prepare a paste.

8. The strengthening and strengthening ointment according to claim 7, characterized in that: The gauze filtering described in step (2) refers to filtering with 3 layers of gauze, 5 layers of gauze, 8 layers of gauze, and 10 layers of gauze in sequence.

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