Method for improving seedling quality of ligustrum sinense l.

Abstract

The application discloses a method for improving seedling quality of continuous fragrant tree. The method combines greenhouse culture with shaking table culture, effectively controls biomass distribution in the growth process of the continuous fragrant tree seedlings, significantly improves the ratio of ground diameter to plant height, improves stem thickness and the proportion of underground biomass, achieves the effect of strong seedlings and improved seedling quality, and provides technical support for population expansion and large-scale utilization of the continuous fragrant tree.

Description

Technical Field

[0001] This invention relates to the field of plant cultivation technology, and more specifically to a method for improving the quality of *Cercidiphyllum japonicum* seedlings. Background Technology

[0002] *Cercidiphyllum japonicum*, a deciduous tree belonging to the genus *Cercidiphyllum* in the family Cercidiphyllaceae, is mainly distributed in southwestern Shanxi, Henan, Shaanxi, Gansu, Anhui, Zhejiang, Jiangxi, Hubei, and Sichuan provinces of my country. It is a relict species of ancient tropical plants from the Tertiary period, belonging to a single family and representing an ancient and primitive woody plant. *Cercidiphyllum japonicum* holds a unique taxonomic position within the plant community, providing important scientific value for understanding the origin and phylogeny of its family. The fruit of *Cercidiphyllum japonicum* can be used medicinally, the bark and leaves can be used to extract tannin, and the leaves can be used to prepare fragrance enhancers. Furthermore, the wood of *Cercidiphyllum japonicum* has straight grain and fine texture, making it both a rare and valuable timber species and an important species for minting coins. Its beautiful tree shape, unique leaf shape, and rich seasonal changes also give it high ornamental value.

[0003] Because *Cercidiphyllum japonicum* is dioecious and produces relatively few fruits, natural regeneration is difficult, resulting in scarce resources and its current endangered status. It is listed in the *List of Rare and Endangered Plants of China*, the *China Plant Red Data Book*, and the *National Key Protected Wild Plants List*, and is a Class II National Key Protected Wild Plant. For many years, researchers have attempted artificial propagation of *Cercidiphyllum japonicum* through various methods such as cuttings, tissue culture, and seed sowing. However, due to the limited nutrients carried by seeds and the demanding requirements of seedlings for their growing environment, the seedling emergence rate is low, and the quality of seedlings is poor. Therefore, cultivating robust seedlings has become a technical bottleneck for the propagation and large-scale utilization of *Cercidiphyllum japonicum*.

[0004] Currently, the main techniques for promoting the growth of *Cercidiphyllum japonicum* seedlings include fertilization, growth regulator application, and spraying with biological agents. However, long-term excessive fertilization can lead to soil acidification, salinization, and soil microbial imbalance. Improper use of growth regulators can affect the growth and development of *Cercidiphyllum japonicum*, and even cause plant deformities. There are many types of biological agents, and improper use of the type and concentration can result in poor plant growth or have adverse effects. Furthermore, the reagents used in the preparation and application of these techniques inevitably pollute the environment and increase carbon emissions.

[0005] Therefore, how to provide a safer, more efficient, and environmentally friendly method to improve the quality of *Cercidiphyllum japonicum* seedlings is a technical problem that urgently needs to be solved by those skilled in the art. Summary of the Invention

[0006] In view of this, the present invention provides a method for improving the quality of Cercidiphyllum japonicum seedlings. The method combines greenhouse cultivation with shaking table cultivation, which effectively regulates the biomass distribution during the growth process of Cercidiphyllum japonicum seedlings, significantly increases the ratio of ground diameter to plant height, and increases the stem thickness and the proportion of underground biomass, thereby achieving the effect of strong seedlings and improving seedling quality.

[0007] To achieve the above objectives, the present invention adopts the following technical solution:

[0008] A method to improve the quality of *Cercidiphyllum japonicum* seedlings involves placing *Cercidiphyllum japonicum* seedlings on a shaker and shaking them at a uniform speed for cultivation. After cultivation, high-quality *Cercidiphyllum japonicum* seedlings are obtained.

[0009] A method for improving the quality of Cercidiphyllum japonicum seedlings includes the following steps:

[0010] (1) Substrate pretreatment and seed sowing: After disinfecting and removing insects from the seedling substrate of *Cercidiphyllum japonicum*, fill it into the seedling container, water it thoroughly, and sow the germinated seeds.

[0011] (2) Seed pre-culture: Place the seedling container after sowing in a greenhouse and culture it under full light until the seedling leaves grow to 4-5 pairs to obtain pre-cultured seedlings;

[0012] (3) Seedling shaking culture: Keep the culture conditions unchanged, place the pre-cultured seedlings on the shaking table after sunset, shake them at a uniform speed, and turn off the shaking table after sunrise. The quality of seedlings can be improved by the above method.

[0013] Preferably, the preparation method of the seedling substrate of *Cercidiphyllum japonicum* in step (1) is as follows: perlite, vermiculite, peat moss and coconut coir are mixed in a volume ratio of 1:1:2:2; the sowing depth of the seeds is 0.5-1 mm.

[0014] Preferably, the greenhouse full-light cultivation conditions in step (2) are as follows: daytime temperature 25-30℃, air humidity 50-80%, nighttime temperature 15-18℃, air humidity 50-80%.

[0015] Further preferred, the greenhouse full-light cultivation conditions in step (2) are as follows: daytime temperature 28℃, air humidity 55%, nighttime temperature 17℃, air humidity 75%.

[0016] Preferably, the shaking culture time in step (3) is 3-10 months, 5-6 days per week, and 8-10 hours per day; the shaking culture speed is 60-90 rpm.

[0017] More preferably, the shaking incubation speed in step (3) is 80 rpm.

[0018] As can be seen from the above technical solution, compared with the prior art, the present invention has the following beneficial effects:

[0019] It is generally believed that seedlings cultivated in open fields are of higher quality than those cultivated in greenhouses, particularly in terms of stem thickness and root development. This is largely due to the lack of wind exposure in greenhouse seedlings. However, greenhouse cultivation offers significant advantages in terms of environmental control and seedling growth rate, which are unmatched by open field cultivation. Therefore, this invention combines greenhouse cultivation with shaking table cultivation. This provides mechanical shaking to the seedlings and effectively controls the cultivation environment, preventing excessive water loss caused by high wind speeds. This achieves effective regulation of biomass distribution in *Cercidiphyllum japonicum* seedlings, significantly increasing the ratio of diameter at breast height to plant height, stem thickness, and the proportion of underground biomass, resulting in robust seedlings and improved seedling quality. Attached Figure Description

[0020] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on the provided drawings without creative effort.

[0021] Figure 1 The attached diagram shows, from left to right: the experimental group, control group 1, and control group 2.

[0022] Figure 2 The attached diagram shows, from left to right, the experimental group, control group 1, and control group 2. Detailed Implementation

[0023] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0024] Example 1

[0025] Effects of Nighttime Shaking on the Growth Traits of Cercidiphyllum japonicum Seedlings

[0026] One hundred uniformly growing *Cercidiphyllum japonicum* seedlings (average height 12cm, average diameter at ground level 1.4mm, average of 4 pairs of leaves per plant) were placed in a full-sunlight greenhouse for one week of acclimatization. The greenhouse conditions were set as follows: daytime temperature 28℃, air humidity 55%; nighttime temperature 17℃, air humidity 75%. After the acclimatization period, the seedlings were divided into an experimental group and a control group 1, with the following conditions:

[0027] Experimental group: After sunset, the seedlings of Cercidiphyllum japonicum along with their seedling containers were placed on a shaking seedbed at 80 rpm and shaken at a uniform speed until sunrise the next day, at which point the shaking bed was turned off.

[0028] Control group 1: Not placed on a shaking seedbed at night;

[0029] The environmental conditions for both groups were consistent with the adaptation and cultivation phases during the experiment, which lasted for 3 months. After the experiment, the plant height, ground diameter, ground diameter to plant height ratio, relative chlorophyll content of leaves, net photosynthetic rate, ratio of underground biomass to aboveground biomass after drying, specific leaf area, and seedling vigor index were statistically analyzed for both treatments. The experimental results are shown in Tables 1-3, and appendix... Figure 1-2 As shown.

[0030] Table 1. Effects of nighttime shaking on the growth traits of *Cercidiphyllum japonicum* seedlings.

[0031] Plant height (cm) Diameter of earth (cm) Ground diameter / plant height experimental group 28.35±2.90 0.41±0.02 0.014±0.001 Control group 1 39.49±4.01* 0.42±0.02 0.011±0.001**

[0032] Note: * indicates that control group 1 is significantly different from the experimental group, and ** indicates that control group 1 is extremely significantly different from the experimental group.

[0033] Table 2 Effects of nighttime shaking on photosynthetic traits of *Cercidiphyllum japonicum* seedlings

[0034] Relative chlorophyll content of leaves <![CDATA[Net photosynthetic rate (μmol·m -2 ·s -1 )]]> experimental group 32.42±2.18 8.96±1.64 Control group 1 30.38±2.58 9.52±1.21

[0035] Table 3. Effects of nighttime shaking on biomass indicators of *Cercidiphyllum japonicum* seedlings

[0036] underground biomass / aboveground biomass <![CDATA[Specific leaf area (cm 2 ·mg -1 )]]> Strong seedling index experimental group 0.32±0.04 0.47±0.03 401.65±66.01 Control group 1 0.28±0.05** 0.46±0.03 427.69±141.69

[0037] Note: ** indicates that the control group 1 was significantly different from the experimental group.

[0038] Results analysis: It can be seen that the ratio of diameter at ground level to plant height and the proportion of underground biomass of the Cercidiphyllum japonicum seedlings shaken at night are significantly higher than those of the control group. However, there are no significant differences in photosynthesis-related indicators, specific leaf area, and seedling vigor index. This indicates that shaking can affect the biomass allocation of Cercidiphyllum japonicum seedlings, allowing more energy to be used to improve stem thickness and underground biomass.

[0039] Example 2

[0040] The effects of nighttime shaking and wind on the growth traits of Cercidiphyllum japonicum seedlings

[0041] One hundred uniformly growing *Cercidiphyllum japonicum* seedlings (average height 12cm, average diameter at ground level 1.4mm, average number of leaves per plant) were placed in a full-sunlight greenhouse for one week of acclimatization. The greenhouse conditions were set as follows: daytime temperature 28℃, air humidity 55%; nighttime temperature 17℃, air humidity 75%. After the acclimatization period, the seedlings were divided into two groups under the following conditions:

[0042] Experimental group: After sunset, the seedlings of Cercidiphyllum japonicum along with their seedling containers were placed on a shaking seedbed at 80 rpm and shaken at a uniform speed until sunrise the next day, at which point the shaking bed was turned off.

[0043] Control group 2: The seedlings were placed on a shaking bed at night, and a fan was used to create a wind effect with a wind speed of 3.0 m / s.

[0044] The environmental conditions for both groups were consistent with the adaptation and cultivation phases during the experiment, which lasted for 3 months. After the experiment, the plant height, ground diameter, ground diameter to plant height ratio, relative chlorophyll content of leaves, net photosynthetic rate, ratio of underground biomass to aboveground biomass after drying, specific leaf area, and seedling vigor index were statistically analyzed for both treatments. The experimental results are shown in Tables 4-5, and appendix... Figure 1-2 As shown.

[0045] Table 4. Effects of nighttime shaking and wind on the growth traits of *Cercidiphyllum japonicum* seedlings

[0046] Plant height (cm) Diameter of earth (cm) Ground diameter / plant height experimental group 28.35±2.90 0.41±0.02 0.014±0.002 Control group 2 23.38±2.77** 0.37±0.02** 0.014±0.002

[0047] Note: ** indicates that the control group was significantly different from the experimental group.

[0048] Table 5 Effects of nighttime shaking and wind on photosynthetic traits of *Cercidiphyllum japonicum* seedlings

[0049] Relative chlorophyll content of leaves <![CDATA[Net photosynthetic rate (μmol·m -2 ·s -1 )]]> experimental group 32.42±2.18 8.96±1.64 Control group 2 30.49±2.93 5.7±0.73**

[0050] Note: ** indicates that the control group was significantly different from the experimental group.

[0051] Table 6. Effects of nighttime shaking and wind on biomass indicators of *Cercidiphyllum japonicum* seedlings.

[0052] underground biomass / aboveground biomass <![CDATA[Specific leaf area (cm 2 ·mg -1 )]]> Strong seedling index experimental group 0.32±0.04 0.47±0.03 401.65±66.01 Control group 2 0.31±0.05 0.42±0.04** 285.01±88.16**

[0053] Note: ** indicates that the control group was significantly different from the experimental group.

[0054] Results analysis: It can be seen that the seedlings of Cercidiphyllum japonicum that were shaken at night were superior to the treatment of shaking + wind in terms of plant height, ground diameter, net photosynthetic rate, specific leaf area and seedling vigor index. This indicates that shaking alone can improve the growth, photosynthetic rate and seedling quality of Cercidiphyllum japonicum seedlings.

[0055] Example 3

[0056] Investigating the effects of different shaking speeds on the growth traits of Cercidiphyllum japonicum seedlings

[0057] Sixty uniformly growing *Cercidiphyllum japonicum* seedlings (average height 12cm, average diameter at ground level 1.4mm, average number of leaves per plant) were placed in a full-sunlight greenhouse for one week of acclimatization. The greenhouse conditions were set as follows: daytime temperature 28℃, air humidity 55%; nighttime temperature 17℃, air humidity 75%. After the acclimatization period, the seedlings were divided into three groups.

[0058] Experimental group: After sunset, the seedlings of Cercidiphyllum japonicum along with their seedling containers were placed on a shaking seedbed at 80 rpm and shaken at a constant speed until sunrise the next day, at which point the shaking bed was turned off.

[0059] Control group 3: Similar to the experimental group, except that the shaking speed was set to 40 rpm;

[0060] Control group 4: Similar to the experimental group, except that the shaking speed was set to 120 rpm;

[0061] The environmental conditions for all three groups were consistent with the adaptation and cultivation phases during the experiment, which lasted for two weeks. After the experiment, the plant height, ground diameter, and the ratio of ground diameter to plant height for the three treatments were statistically analyzed. The experimental results are shown in Table 7.

[0062] Table 7. Effects of shaking speed on growth traits of *Cercidiphyllum japonicum* seedlings

[0063] Plant height (cm) Diameter of earth (cm) Ground diameter / plant height experimental group 29.61±3.27 0.45±0.15 0.015±0.002 Control group 3 35.74±2.54* 0.42±0.02 0.012±0.001* Control group 4 30.68±4.67 0.38±0.11 0.012±0.001*

[0064] Note: * indicates a significant difference between the control group and the experimental group.

[0065] Results analysis: It was observed that the ratio of ground diameter to plant height of seedlings in control group 3 was significantly different from that in the experimental group. In control group 4, the root system of all plants was exposed due to most of the substrate being thrown out, which seriously affected the growth of seedlings, and the ratio of ground diameter to plant height was also significantly lower than that in the experimental group.

[0066] The various embodiments in this specification are described in a progressive manner, with each embodiment focusing on the differences from other embodiments. The same or similar parts between the various embodiments can be referred to each other.

[0067] The above description of the disclosed embodiments enables those skilled in the art to make or use the invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the invention. Therefore, the invention is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A method for improving the quality of seedlings of Lignum Aquilariae Resinatum, characterized in that, The seedlings of the Lindera communis are placed on a shaking bed, and shaken at night, and the shaking speed is 80 rpm, and high quality seedlings of the Lindera communis are obtained.

2. The method for improving the seedling quality of Lindera communis according to claim 1, characterized in that, Specifically, the method comprises the following steps: (1) substrate pretreatment and seed sowing: after the seedling substrate of the Lindera communis is disinfected and de-insected, the substrate is filled into a seedling container, watered, and the germinated seeds are sowed; (2) seed pre-culture: the seedling container after sowing is placed in a greenhouse, and full light culture is carried out until the seedling leaves grow to 4-5 pairs, and pre-cultured seedlings are obtained; (3) seedling shaking culture: the pre-cultured seedlings are placed on a shaking bed after sunset, and uniform shaking culture is carried out, and the shaking bed is turned off after sunrise, and the seedling quality is improved through the above method.

3. The method of claim 2, wherein, The Lindera communis seedling substrate preparation method in step (1) is as follows: perlite, vermiculite, peat and coconut husk are mixed according to a volume ratio of 1:1:2:2 to prepare the Lindera communis seedling substrate; and the sowing depth of the seeds is 0.5-1 mm.

4. The method of claim 2, wherein, The greenhouse full light culture conditions in step (2) are as follows: the daytime temperature is 25-30℃, the air humidity is 50-80%, the nighttime temperature is 15-18℃, and the air humidity is 50-80%.

5. The method of claim 2, wherein, The greenhouse full light culture conditions in step (2) are as follows: the daytime temperature is 28℃, the air humidity is 55%, the nighttime temperature is 17℃, and the air humidity is 75%.

6. The method of claim 2, wherein, The shaking culture time in step (3) is 3-10 months, 5-6 days per week, and 8-10 hours per day.

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