A pesticide composition for controlling field pests

Abstract

The present invention relates to the technical field of pesticides, and particularly relates to a pesticide composition for controlling field pests. Its active ingredients are compounded by emamectin benzoate and pogostone, wherein: the mass ratio of emamectin benzoate to pogostone is 1:20 - 8. When emamectin benzoate and pogostone are compounded in the pesticide composition of the present invention, they show a synergistic effect within a certain mass ratio range, which can improve the control effect. Based on this, the application dosage of chemical agents can be reduced, and problems such as pesticide residues and environmental pollution caused by large-dose application can be avoided to a certain extent.

Description

Technical Field

[0001] The present invention relates to the technical field of pesticides, and particularly relates to a pesticide composition for controlling field pests. Background Art

[0002] The Spodoptera litura Fabricius belongs to the Noctuidae of Lepidoptera, also known as the lotus leaf roller, commonly known as the noctuid, aconite worm, etc., and is a polyphagous and voracious pest with a worldwide distribution. It is reported that the host plants that Spodoptera litura can damage reach 389 species in 109 families. The Spodoptera litura damages with larvae. The young larvae feed on the mesophyll, and the damaged leaves only remain a layer of epidermal tissue like a window screen; the older larvae eat the leaves into notches, and when severe, the whole leaf is eaten up leaving only the main vein, causing serious losses to agricultural production. At present, the method for controlling Spodoptera litura mainly relies on chemical control. In the actual production process, a single agent is likely to cause pests to develop drug resistance, resulting in a reduction in the control effect; while increasing the dosage of the pesticide is likely to lead to a series of problems such as pesticide residues and environmental pollution.

[0003] Patchouli ketone is a compound isolated and purified from patchouli essential oil with insecticidal activity. It is a colorless needle crystal with the molecular formula C 12 H 16 O4, and the molecular weight is 224.257. Its structural formula is as follows:

[0004]

[0005] "Insecticidal effects of patchouli essential oil and analysis of its active ingredients (Zeng Qingqian et al., Journal of Plant Resources and Environment, 2006, 15(3): 21-25)" discloses that patchouli essential oil and patchouli ketone have good control effects on the larvae of Pieris rapae and Plutella xylostella. Feeding experiments were carried out on the larvae of Pieris rapae and Plutella xylostella with 2.5 - 40 mg / mL patchouli essential oil and 1 - 10 mg / mL patchouli ketone respectively, and both showed a strong antifeedant effect; the LC 50 values of the toxic effects of patchouli essential oil and patchouli ketone on the 4th instar larvae of Pieris rapae were 104.28 μg / mL and 32.20 μg / mL respectively.

[0006] "Biological activity of patchouli ketone against Spodoptera litura and its effects on detoxifying enzymes (Zhang Jiahui et al., South China Agricultural University, Master's thesis, June 1, 2016)" discloses that patchouli ketone has stomach toxicity, contact toxicity, antifeedant effect and growth inhibition effect on the larvae of Spodoptera litura, and has the prospect of being developed into an insecticide, and it is speculated that carboxylesterase may be an important site for patchouli ketone to act on Spodoptera litura.

[0007] "Study on the Chemical Synthesis Process of Pogostone and Its Insecticidal Activity Against Lepidopteran Pests (Huang Sihan et al., Guangzhou University of Chinese Medicine, Master's Thesis, April 1, 2014)" discloses that pogostone has a strong antifeedant effect on Spodoptera litura and Spodoptera exigua, and has good insecticidal activity against the two pests. Among them, the gastric toxicity LC 50 of Spodoptera litura and Spodoptera exigua are 986.88 mg / L and 545.61 mg / L respectively, and the contact toxicity LC 50 are 1041.42 mg / L and 519.48 mg / L.

[0008] Mixing different pesticide varieties is a common method for developing new pesticides. In most cases, the mixing of pesticide varieties is additive, and there are few mixtures with synergistic effects. The inventors found through a large number of indoor experiments that when metaflumizone, spinetoram, emamectin benzoate or chlorantraniliprole are mixed with pogostone, they show a synergistic effect on Spodoptera litura.

[0009] Currently, there are no relevant reports on the mixture of metaflumizone, spinetoram, emamectin benzoate or chlorantraniliprole and pogostone. Summary of the Invention

[0010] The purpose of the present invention is to provide a pesticide composition for controlling field pests, so as to solve the problems that the use of a single agent is likely to cause pests to develop drug resistance, resulting in a reduction in control efficacy; and a series of problems such as an increase in the dosage of pesticides is likely to cause pesticide residues and environmental pollution.

[0011] To achieve the above object, the present invention provides the following technical solutions:

[0012] A pesticide composition for controlling field pests, the active ingredients of which are composed of a mixture of metaflumizone, spinetoram, emamectin benzoate or chlorantraniliprole and pogostone, wherein:

[0013] The mass ratio of metaflumizone to pogostone is 4 - 7:9 - 1;

[0014] The mass ratio of spinetoram to pogostone is 1 - 40:3 - 1;

[0015] The mass ratio of emamectin benzoate to pogostone is 1:20 - 8;

[0016] The mass ratio of chlorantraniliprole to pogostone is 1 - 30:10 - 1.

[0017] Preferably, the mass ratio of emamectin benzoate to pogostone is 1:10.

[0018] The present invention also provides a pesticide formulation, which is prepared from the pesticide composition for controlling field pests and auxiliary components acceptable in pesticide science.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] When metaacetaldehyde, spinetoram, emamectin benzoate or chlorantraniliprole in the pesticide composition of the present invention is compounded with pogostone, it shows a synergistic effect within a certain mass ratio range, which can improve the control effect on Spodoptera litura. Based on this, the application dosage of chemical agents can be reduced, and problems such as pesticide residues and environmental pollution caused by large-dose application can be avoided to a certain extent. Specific Embodiments

[0021] According to the following embodiments, the present invention can be better understood. The content described in the embodiments is only used to illustrate the present invention and should not and will not limit the present invention described in detail in the claims.

[0022] Embodiment : Indoor bioactivity test of the compounding of pogostone

[0023] 1. Test object: Spodoptera litura Fabricius, collected from rape fields in the suburbs of Zhengzhou, Henan. It was continuously reared for multiple generations with fresh and clean rape leaves in the laboratory, and 3rd instar larvae of the same size and health were selected as the test objects.

[0024] 2. Test agents

[0025] 97% metaacetaldehyde technical (Hebei Xingbai Agricultural Science and Technology Co., Ltd.)

[0026] 81.2% spinetoram technical (Corteva Agriscience Co., Ltd.)

[0027] 90% emamectin benzoate technical (Jiangsu Fengyuan Bioengineering Co., Ltd.)

[0028] 94% chlorantraniliprole technical (Xiangshui Zhongshan Biotechnology Co., Ltd.)

[0029] 98% pogostone technical (Shanghai Yuanye Bio-Technology Co., Ltd.)

[0030] The technicals were first dissolved in dimethyl sulfoxide to prepare single-agent mother liquors, and then diluted with an aqueous solution of 0.1% Tween-80. Multiple groups of ratios were set, and 7 gradient mass concentrations were set for each single agent and the mixed agents of each group ratio according to the equal ratio method. 50 mL of the liquid medicine at each mass concentration was reserved.

[0031] 3. Test method: (Refer to "Guidelines for Indoor Bioassay of Pesticides - Part 6: Immersion Method for Insecticidal Activity Test", NY / T 1154.6 - 2006)

[0032] Immerse the test insects in the liquid medicine for 10 s. After sucking up the excess liquid medicine with filter paper, transfer them to be reared under normal conditions. Each treatment is repeated 4 times, with 20 insects immersed in each repetition. Set a treatment containing only dimethyl sulfoxide and 0.1% Tween - 80 as the blank control. Observe the death situation of the test insects 24 h after treatment, record the total number of insects and the number of dead insects in each treatment respectively, and calculate the corrected mortality of each treatment based on these data.

[0033]

[0034] In the above formula: P - Mortality rate, unit: %; K - Number of dead insects; N - Total number of insects in the treatment.

[0035]

[0036] In the above formula: P1 - Corrected mortality rate, unit: %; P t - Mortality rate of the treatment, unit: %; P0 - Mortality rate of the blank control, unit: %.

[0037] 4. Data analysis: Use DPS software to conduct regression analysis on the logarithm of the concentration of each treatment agent and the probit value of the corrected mortality rate of each treatment, calculate the LC 50 of each treatment agent, and calculate the co - toxicity coefficient (CTC value) of the mixture according to Sun Yunpei method.

[0038]

[0039] In the above formula: ATI - Measured toxicity index of the mixture; S - LC 50 of the standard agent, unit: mg / L; M - LC 50 of the test agent, unit: mg / L.

[0040] TTI = TI A ×P A +TI B ×P B

[0041] In the above formula: TTI - Theoretical toxicological index of the mixture; TI A - Toxicity index of agent A; P A - Percentage content of agent A in the mixture, unit: percentage (%) ; TI B - Toxicity index of agent B; P B - Percentage content of agent B in the mixture, unit: percentage (%).

[0042]

[0043] In the above formula: CTC--co-toxicity coefficient; ATI--actual toxicity index of the mixture; TTI--theoretical toxicity index of the mixture.

[0044] 5. Efficacy evaluation

[0045] The synergistic effect of the drug was evaluated based on the calculated co-toxicity coefficient (CTC), CTC ≤ 80 for antagonistic effect, 80 < CTC < 120 for additive effect, and CTC ≥ 120 for synergistic effect. The results are shown in Tables 1-4.

[0046] Table 1 The results of the toxicity test of the combination of metaflumizone and patchouli ketone against Spodoptera litura

[0047]

[0048] As can be seen from Table 1, within the mass ratio range of 4-7:9-1, the co-toxicity coefficient of metaflumizone and patchouli ketone to Spodoptera litura was greater than 120, showing a synergistic effect.

[0049] Table 2 The results of the toxicity test of ethyl spinetoram and patchouli ketone against Spodoptera litura

[0050]

[0051] As can be seen from Table 2, within the mass ratio range of 1-40:3-1, the co-toxicity coefficient of ethyl spinetoram and patchouli ketone to Spodoptera litura was greater than 120, showing a synergistic effect.

[0052] Table 3 The results of toxicity test of emamectin benzoate and patchouli ketone against Spodoptera litura

[0053]

[0054] As can be seen from Table 3, within the mass ratio range of 1:20-8, the co-toxicity coefficient of avermectin benzoate and patchouli ketone to Spodoptera litura was greater than 120, showing a synergistic effect; especially when the mass ratio was 1:10, the co-toxicity coefficient reached 908.02, and the synergistic effect was particularly significant.

[0055] Table 4 The results of toxicity test of chlorantraniliprole and patchouli ketone against Spodoptera litura

[0056]

[0057] It can be seen from Table 4 that within the mass ratio range of 1-30:10-1, the co-toxicity coefficient of chlorantraniliprole and patchouli ketone to Spodoptera litura was greater than 120, showing a synergistic effect.

[0058] In summary, when metaflumizone, spinetoram, emamectin benzoate or chlorantraniliprole is compounded with pogostone in the pesticide composition of the present invention, it shows a synergistic effect within a certain mass ratio range, which can improve the control effect on Spodoptera litura. Based on this, the application dosage of chemical agents can be reduced, and problems such as pesticide residues and environmental pollution caused by large-dose application can be avoided to a certain extent.

[0059] The above description is only the preferred embodiment of the present invention. For those skilled in the art, without departing from the principle of the present invention, appropriate improvements can be made, and these improvements are also within the protection scope of the present invention.

Claims

1. A pesticide composition for controlling field pests, characterized in that, Its active ingredient is composed of emamectin benzoate and pogostone, wherein: the mass ratio of emamectin benzoate to pogostone is 1:20 - 8.

2. The pesticide composition for controlling field pests according to claim 1, characterized in that, The mass ratio of emamectin benzoate to pogostone is 1:

10.

3. A pesticide preparation, characterized in that, The pesticide preparation is prepared from the pesticide composition for controlling field pests described in claim 1 and auxiliary ingredients acceptable in pesticide science.

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