A wireless optical recording system

Abstract

The present application relates to a kind of wireless optical fiber recording system, belongs to the scientific research instrument of neurosciences field, especially relates to calcium imaging recording technology, solve the problem of large volume in prior art optical fiber recording system, signal transmission is unstable.The present application includes host computer, wireless sensor, DAQ host and insert core needle, one end of the insert core needle is connected with the small animal to be measured, the other end is connected with the wireless sensor, the DAQ host is wirelessly connected with the wireless sensor.The signal transmission of the wireless optical fiber recording system of the present application is stable, recording result precision is high, and overall structure is small.

Description

Technical Field

[0001] This invention relates to scientific research instruments in the field of neuroscience, particularly to calcium imaging recording technology, and especially to a wireless fiber optic recording system. Background Technology

[0002] In recent years, fiber optic recording systems have become increasingly popular in neuroscience as a technique for recording neuronal activity in specific brain nuclei of freely moving animals. Compared to traditional electrophysiological recording methods, fiber optic recording systems offer advantages such as strong resistance to electromagnetic interference, stable recording, and longer recording times. Compared to two-photon microscopy, fiber optic recording systems do not require anesthesia or head fixation during use and can record deep brain regions. Therefore, fiber optic recording systems play a crucial role in neurological research.

[0003] Existing fiber optic recording systems are wired and mainly consist of host computer software, a recording host (i.e., the DAQ host), fiber optic patch cords, and ferrules. The host computer software and the recording host are connected via USB. The recording host internally houses a light source system, photoelectric sensors, and a data acquisition card (i.e., the motherboard). The light source output of the recording host is connected to a fiber optic patch cord, the other end of which is connected to a ferrule implanted inside the animal's brain.

[0004] Existing fiber optic recording systems all require fiber optic patch cords for connection, which can interfere with the behavior of laboratory animals, such as reducing their range of motion, making it impossible to perform some tubular experiments, and also posing a risk of fiber optic entanglement. Using lasers as a light source would increase the size of the DAQ (Data Acquisition) unit, and the laser device, photoelectric sensors, and data acquisition card all being located within the DAQ unit would require higher heat dissipation. Furthermore, the connectivity requirements between the light source system, patch cords, and ferrules are high; improper coupling can lead to damage to components in the optical path, loss of transmitted signals, or inaccurate recording. In addition, the optical signal must be transmitted to the photoelectric sensor via patch cords, resulting in a long transmission path that can cause signal instability. Patch cord transmission also introduces interference because the patch cords themselves have a fluorescence effect; when the light reaches the fiber optic cable, the fiber's fluorescence effect can cause interference. Summary of the Invention

[0005] Based on the above analysis, the present invention aims to provide a wireless optical fiber recording system to solve the problems of large size and unstable signal transmission in existing optical fiber recording systems.

[0006] This invention provides a wireless fiber optic recording system, including a host computer, a wireless sensor, a DAQ host, and a pin. One end of the pin is connected to the small animal to be tested, and the other end is connected to the wireless sensor. The DAQ host is wirelessly connected to the wireless sensor.

[0007] Furthermore, the wireless sensor includes a light source, a dichroic mirror, and a self-focusing lens arranged sequentially along a first axis, and a photoelectric conversion element and a second filter arranged sequentially along a second axis, wherein the first axis is perpendicular to the second axis, and the second axis passes through the dichroic mirror.

[0008] Furthermore, the wireless sensor also includes a first filter, which has a base surface, one side of which is a smooth surface and the other side is an embossed surface.

[0009] Furthermore, the dichroic mirror is set at 45° to both the first axis and the second axis.

[0010] Furthermore, it also includes a sensor housing, in which the light source, dichroic mirror, self-focusing lens, second filter, and photoelectric conversion element are all disposed.

[0011] Furthermore, the ferrule includes a ceramic head and a single-mode optical fiber. One end of the single-mode optical fiber is implanted into the skull of the small animal, and the other end is located inside the ceramic head. One end of the ceramic head is fixed to the head of the small animal, and the other end is located inside the wireless sensor.

[0012] Furthermore, the single-mode optical fiber is arranged along the first axis.

[0013] Furthermore, the lower end of the ceramic head is provided with a circumferential groove.

[0014] Furthermore, the DAQ host connects to and controls the wireless sensors via a wireless module, and is capable of controlling at least two of the wireless sensors simultaneously.

[0015] Furthermore, it also includes a charging module that provides power and storage space for the wireless sensor.

[0016] Furthermore, the host computer is used to control the wireless sensor and / or the DAQ host, and to collect, transfer, analyze, input, and output the fiber optic recorded data.

[0017] Compared with the prior art, the present invention can achieve at least one of the following beneficial effects:

[0018] (1) This invention employs wireless transmission and control, eliminating the need for cable connections between the wireless sensor and the DAQ host. This not only improves system convenience and reduces interference with animal behavior, allowing small animals to move freely during experiments and effectively reducing activity range restrictions, but also offers a natural advantage in enclosed experimental settings. It enables researchers to conduct pipeline-based behavioral experiments. Furthermore, by eliminating fiber optic jumpers, it improves light transmission efficiency within the system, reduces interference in signal recording, and enhances recording accuracy, resulting in more objective experimental results.

[0019] (2) This invention moves the complex optical structure from the original DAQ host to a more forward-facing wireless sensor, directly acting on the brain of a small animal (mouse). It is small and lightweight (weight less than or equal to 3g, volume less than or equal to 2 cubic centimeters), and will not affect the mouse's activity. The wireless sensor achieves an accuracy of 1uW (optical power). Through precise optical path design, it ensures the incident light of the required wavelength band and reflects light of other wavelength bands, thereby improving the sensor's sensitivity.

[0020] (3) This invention adds Bluetooth transmission, which controls the wireless sensor and receives data via Bluetooth, achieving wireless transmission and eliminating the need for fiber optic patch cords. The original bulky recording host, which includes a light source system, optical sensors, and data transmission, is quite large. The DAQ host of this invention removes the optical structure and becomes a lighter DAQ host. Therefore, the DAQ host can be made smaller, and its main purpose is data interaction, receiving data from the wireless sensor and sending it to the host computer. Its function becomes more singular, and data processing and transmission are more accurate and faster.

[0021] (4) The wireless sensor of the present invention is active, contains a battery, has a battery life of more than or equal to 1 hour, and can be recharged by a charging module after use. In the existing wired fiber optic recording system, the jumper itself has a fluorescence effect during use. When light is transmitted to the fiber optic cable, the fluorescence effect of the fiber itself will cause interference. In order to avoid the interference of the fiber optic jumper, the prior art adds a reference light, which makes the optical path structure of the entire system complex, increases the size, and makes the subsequent data processing complicated. However, the wireless fiber optic recording system of the present invention does not need to use jumpers and reference light, so it avoids this interference and can make the results more accurate.

[0022] (5) This invention eliminates the need for fiber optic patch cords (including extension cords and slip rings), directly connecting the wireless sensor to the ferrule and communicating and controlling via a wireless Bluetooth module. By removing the original structure that transmitted light via fiber optic patch cords, the optical structure is miniaturized and placed within a small wireless sensor on the mouse's head, avoiding transmission interference caused by the original patch cords. Calcium imaging signals can be directly acquired from the nearby wireless sensor, making data acquisition more accurate and transmission more effective. Furthermore, since patch cords are not required, the adverse factors that restrict mouse behavior caused by patch cords are eliminated.

[0023] (6) The present invention can eliminate the first filter, making the optical path more concise and minimizing the impact on light; at the same time, it makes the wireless sensor lighter and smaller.

[0024] In this invention, the above-described technical solutions can be combined with each other to achieve more preferred combinations. Other features and advantages of this invention will be set forth in the following description, and some advantages may become apparent from the description or be learned by practicing the invention. The objects and other advantages of this invention can be realized and obtained from what is particularly pointed out in the description and drawings. Attached Figure Description

[0025] The accompanying drawings are for illustrative purposes only and are not intended to limit the invention. Throughout the drawings, the same reference numerals denote the same parts.

[0026] Figure 1 This is a schematic diagram of the wireless sensor structure in a specific embodiment;

[0027] Figure 2 This is a schematic diagram of the DAQ host structure in a specific embodiment;

[0028] Figure 3 This is a schematic diagram of the insert pin structure in a specific embodiment;

[0029] Figure 4 This is a schematic diagram of the charging module structure in a specific embodiment;

[0030] Figure 5 A cross-sectional view of a wireless sensor according to a specific embodiment;

[0031] Figure 6 An exploded view of the wireless sensor in a specific embodiment;

[0032] Figure 7 This is a schematic diagram of the sensor housing structure in a specific embodiment;

[0033] Figure 8 This is a cross-sectional view of the sensor housing in a specific embodiment;

[0034] Figure 9 This is a schematic diagram illustrating the connection between the wireless sensor and the ferrule in a specific embodiment;

[0035] Figure 10 This is a schematic diagram illustrating the usage status of the wireless sensor and the insert pin in a specific embodiment.

[0036] Figure 11 This is an exploded view of the DAQ host in a specific embodiment;

[0037] Figure 12 An exploded view of the ferrule pins in a specific embodiment;

[0038] Figure 13 This is a cross-sectional view of the ferrule pin in a specific embodiment;

[0039] Figure 14 Exploded view (I) of the charging module in a specific embodiment;

[0040] Figure 15 This is a schematic diagram of the lower box top cover structure in a specific embodiment;

[0041] Figure 16 This is a top view of the lower box cover in a specific embodiment;

[0042] Figure 17 A schematic diagram of the third motherboard structure in a specific embodiment (I);

[0043] Figure 18 This is a schematic diagram of the lower box body structure in a specific embodiment;

[0044] Figure 19 This is a schematic diagram showing the connection between the lower housing and the wireless sensor in a specific embodiment;

[0045] Figure 20 Exploded view (II) of the charging module in a specific embodiment;

[0046] Figure 21 A schematic diagram of the lower box structure of a specific embodiment (I);

[0047] Figure 22 This is a schematic diagram of the lower box structure in a specific embodiment (II);

[0048] Figure 23 This is a cross-sectional view of the lower box body in a specific embodiment;

[0049] Figure 24 This is an exploded view of the cap in a specific embodiment;

[0050] Figure 25 This is a schematic diagram showing the connection between the lower box and the limiting post in a specific embodiment;

[0051] Figure 26 This is a schematic diagram (II) of the third motherboard structure in a specific embodiment;

[0052] Figure 27 This is a schematic diagram showing the connection between the lower housing and the wireless sensor and ceramic head in a specific embodiment.

[0053] Figure 28 This is a schematic diagram of the collimation surface relief type micro / nano device design in a specific embodiment.

[0054] Figure label:

[0055] 1-Wireless sensor; 11-Sensor housing; 111-Stepped hole; 112-First slot; 113-First connection hole; 114-First cylindrical hole; 115-Second cylindrical hole; 116-Third cylindrical hole; 117-Second slot; 118-First side; 119-Third slot; 110-Fourth slot; 120-Second connection hole; 121-Second side; 122-Strip hole; 123-Wire groove; 124-Battery slot; 125-First mounting slot; 126-Side cover; 127-Third side; 128-Second mounting slot; 129-Top cover; 130-First mounting part; 131-Second mounting part; 132-Third mounting part; 133-Fourth mounting part;

[0056] 10-First battery; 12-Light source; 13-First filter; 14-Dichroic mirror; 15-Self-focusing lens; 16-Sleeve; 17-Second filter; 18-Photoelectric conversion element; 19-Second motherboard;

[0057] 2-DAQ main unit; 21-Upper cover of the outer casing; 22-First motherboard; 23-Lower cover of the outer casing; 231-First groove; 232-Second groove; 3-Flange pin; 31-Ceramic head; 32-Single-mode fiber; 33-Circumferential groove;

[0058] 4-Charging module; 41-Top cover; 42-Lower housing; 421-Lower housing top cover; 4211-Sensor receiving slot; 4212-Charging spring pin hole; 4213-Wake-up switch hole; 4214-Charging indicator hole; 4215-Protrusion; 4216-Battery level indicator hole; 422-Lower housing body; 4221-Receiving cavity; 4222-USB charging port; 423-Lower housing bottom cover;

[0059] 424-Limiting body; 4241-Limiting post; 42411-Spindle mounting hole; 42412-T-shaped hole; 42413-Second magnetic receiving slot; 42414-Second magnetic component; 4242-Spindle; 4243-Cover; 42431-Connecting block; 42432-Top plate receiving slot; 42433-First magnetic receiving slot; 42434-First magnetic component; 42435-Cover top plate; 42436-Elastic component; 43-Third main board; 431-Wake-up switch; 432-Charging indicator light; 433-Charging spring pin; 434-USB interface; 435-Battery level indicator light; 44-Second battery;

[0060] 100 - Small animals; 200 - Dental cement. Detailed Implementation

[0061] The preferred embodiments of the present invention will now be described in detail with reference to the accompanying drawings, which constitute a part of the present invention and are used together with the embodiments of the present invention to illustrate the principles of the present invention, but are not intended to limit the scope of the present invention.

[0062] In the description of the embodiments of the present invention, it should be noted that, unless otherwise explicitly specified and limited, the term "connected" should be interpreted broadly. For example, it can refer to a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium. Those skilled in the art can understand the specific meaning of the above terms in the present invention according to the specific circumstances.

[0063] Throughout the text, the terms “top,” “bottom,” “above,” “below,” and “on top” refer to the relative positions of components of the device, such as the relative positions of the top and bottom substrates within the device. It is understood that the device is multifunctional and independent of its spatial orientation.

[0064] Example 1

[0065] A specific embodiment of the present invention, such as Figures 1 to 28 As shown, a wireless fiber optic recording system is disclosed, including a host computer, a wireless sensor 1, a DAQ host 2, and a pin 3. The wireless sensor 1 is located outside the DAQ host 2. One end of the pin 3 is connected to the small animal 100 to be tested, and the other end is connected to the wireless sensor 1. The DAQ host 2 is wirelessly connected to the wireless sensor 1.

[0066] Compared to existing technologies, the wireless fiber optic recording system provided in this embodiment uses wireless transmission and control, eliminating the need for cable connections between the wireless sensor and the DAQ host. This not only improves system convenience and reduces interference with animal behavior, allowing small animals (exemplarily mice) to move freely during experiments and effectively reducing activity range restrictions, but also offers natural advantages in enclosed experimental environments. It enables researchers to conduct pipeline-based behavioral experiments. Furthermore, by eliminating fiber optic jumpers, it improves light transmission efficiency within the system, reduces interference in signal recording, and enhances recording accuracy, resulting in more objective experimental results. Simultaneously, the wireless sensor in this embodiment is located outside the DAQ host, effectively reducing the host's size and improving its heat dissipation.

[0067] The DAQ host 2 is dedicated to connecting to and controlling the wireless sensor 1, and receiving data from the wireless sensor 1. When the DAQ host 2 is connected to a host computer, the host computer controls the DAQ host 2, and the wireless sensor 1 is controlled via the wireless module on the first motherboard 22. One DAQ host 2 can control at least two wireless sensors 1 simultaneously.

[0068] In a preferred embodiment of the present invention, the wireless sensor 1 is located outside the DAQ host 2 and is wirelessly connected to the DAQ host 2. The wireless sensor 1 includes a sensor housing 11, which houses an excitation optical path, a collection optical path, and a battery. The sensor housing 11 also has an electrical connection terminal, through which the battery can be charged. The battery is preferably a rechargeable battery, which powers the wireless sensor 1. The excitation optical path mainly includes a light source 12, a dichroic mirror 14, and a self-focusing lens 15. The collection optical path mainly includes a self-focusing lens 15, a dichroic mirror 14, a second filter 17, and a photoelectric conversion element 18. The wireless sensor 1 has a sleeve 16, through which the wireless sensor 1 is connected to a ferrule 3. This allows the self-focusing lens 15 in the wireless sensor 1 to be coupled to the single-mode optical fiber 32 on the ferrule 3.

[0069] Furthermore, the electrical connection terminal can be a USB charging port, through which the wireless sensor 1 can be implemented; alternatively, the electrical connection terminal can also be a charging contact, through which the wireless sensor 1 can be charged. Both charging via USB charging port and charging via charging contact are existing technologies and will not be elaborated upon here.

[0070] In use, the excitation light emitted by the light source 12 passes through the dichroic mirror 14, and then is transmitted through the dichroic mirror 14 again. Finally, it is transmitted through the self-focusing lens 15 to the single-mode optical fiber 32, and then to the target tissue. The target tissue will produce fluorescence when stimulated by the excitation light. The specific path of the fluorescence through the collection optical path can be selected as follows: the fluorescence enters from the single-mode optical fiber 32, is transmitted through the self-focusing lens 15 to the dichroic mirror 14, the fluorescence is reflected by the dichroic mirror 14 to the second filter 17, and finally the fluorescence reaches the photoelectric conversion element 18. The photoelectric conversion element 18 converts the received optical signal into an electrical signal, which is wirelessly transmitted to the DAQ host 2 and / or the host computer. Furthermore, the excitation optical path may also include a first filter 13, which is disposed between the light source 12 and the dichroic mirror 14.

[0071] Optionally, the light transmission path in the excitation optical path includes a first optical path and a second optical path, which are offset from each other, for example, the first optical path and the second optical path have different axes of rotation. Specifically, before the excitation light passes through the dichroic mirror 14, the excitation light emitted by the light source 12 passes through the dichroic mirror 14 and reaches the surface of the dichroic mirror 14. In this process, the excitation light is transmitted along the first optical path. After passing through the dichroic mirror 14, the excitation light will be transmitted along the second optical path, and the excitation light on the second optical path will reach the target tissue along the self-focusing lens 15 and the single-mode optical fiber 32. Further, the second optical path has an offset angle compared to the first optical path. The magnitude and direction of the offset angle are related to the transmittance and refractive index of the dichroic mirror 14. In this invention, by offsetting the light path, the optical path loss caused by the excitation light during transmission can be reduced, making the light intensity reaching the target tissue more stable and the light signal stronger.

[0072] Specifically, the DAQ host 2 includes an upper cover 21, a first motherboard 22, and a lower cover 23. The upper cover 21 is disposed on the lower cover 23 to form the DAQ host casing, and the first motherboard 22 is disposed inside the DAQ host casing.

[0073] In this embodiment, the top of the lower cover 23 is provided with a first groove 231, the middle of the first groove 231 is provided with a second groove 232, and threaded holes are provided at the four corners of the first groove 231. The bottom of the upper cover 21 is provided with a protrusion, and a third groove is provided in the protrusion. When the upper cover 21 is placed on the lower cover 23, the first main board 22 is located in the third groove and is connected to the lower cover 23 by screws. The lower end of the upper cover 21 is located in the first groove 231, and the protrusion is located between the first groove 231 and the first main board 22.

[0074] Furthermore, the DAQ host 2 communicates wirelessly with the wireless sensor 1 (e.g., Bluetooth), and one DAQ host 2 can simultaneously control at least two wireless sensors 1. Furthermore, the DAQ host 2 includes multiple input / output signals and multiple indicator lights. Optionally, the indicator lights include: one DAQ host power indicator light, lit to indicate that the DAQ host 2 is powered on and running; two Bluetooth channel indicator lights, each corresponding to one wireless sensor 1, lit to indicate that the DAQ host 2 is in Bluetooth communication connection with the corresponding wireless sensor 1; and one fault indicator light, lit to indicate that a predicted fault has occurred in the DAQ host 2.

[0075] Optionally, the DAQ host 2 is electrically connected to the host computer via a data cable (e.g., via a USB cable), and the host computer is wirelessly connected to the wireless sensor 1. The DAQ host 2 responds to commands from the host computer and simultaneously feeds back data from the wireless sensor 1 to the host computer. The host computer is used to control the wireless sensor 1 and / or the DAQ host 2, and to collect, transfer, analyze, and output the fiber optic recorded data. Optionally, the host computer can control the Bluetooth pairing between the DAQ host 2 and the wireless sensor 1, and allows the storage of two default MAC addresses of the wireless sensor 1; the host computer controls the on / off state of the wireless sensor 1, such as the on / off state of an LED light, and adjusts the brightness in units of uW, ranging from 0-100 uW. Furthermore, the host computer software has the function of configuring LED brightness. The host computer receives data (i.e., light intensity data) from the wireless sensor 1 transmitted by the DAQ host 2, and can display data information from both wireless sensors 1. It allows adjustment of digital gain and displays the data in an interface chart, and also allows saving the records to a file. Further, the host computer allows connection to an external behavioral camera, can display camera images, and can save the video. The host computer allows loading and replaying saved light intensity records and behavioral videos. The host computer can also control whether the DAQ host 2 responds to input / output. The host computer can also perform noise reduction processing on the light intensity data.

[0076] The wireless sensor 1 is used to access the insert pin 3 implanted in the brain of an animal (preferably a mouse) to record the neural activity in the animal's brain region, and also to communicate wirelessly with the DAQ host 2.

[0077] Specifically, the wireless sensor 1 includes a sensor housing 11, the top of which is provided with a stepped hole 111. The stepped hole 111 is formed by two slots, specifically an upper slot and a lower slot, wherein the projection of the lower slot onto the upper slot is a portion of the end face of the upper slot, that is, a step is formed between the upper and lower slots. Preferably, the upper slot of the stepped hole 111 is a rectangular hole, and the lower slot of the stepped hole 111 is a cylindrical hole, which connects to the middle position of the lower end of the upper slot.

[0078] A first slot 112 is provided below the stepped hole 111 and communicates with the stepped hole 111. The bottom of the stepped hole 111 is connected to a part of the first slot 112, that is, there is a step between the bottom end of the stepped hole 111 and the first slot 112. Preferably, the first slot 112 is a rectangular hole, and the lower end of the stepped hole 111 is connected to the middle of the first slot 112.

[0079] It is worth noting that the first slot 112 is connected to the first side 118 of the sensor housing 11 so as to accommodate the first filter 13.

[0080] A first connecting hole 113 is provided at the lower end of the first slot 112, and the first connecting hole 113 communicates with the first slot 112. Preferably, the first connecting hole 113 is a cylindrical hole, and the first connecting hole 113 is concentric with the lower slot of the stepped hole 111. The diameter of the first connecting hole 113 is smaller than the diameter of the lower slot.

[0081] A first cylindrical hole 114 communicating with the lower end of the first connecting hole 113 is provided. The first cylindrical hole 114 is eccentrically disposed from the first connecting hole 113. Specifically, the axis of the first cylindrical hole 114 is offset toward the outside of the sensor housing 11.

[0082] A second cylindrical hole 115 is provided at the lower end of the first cylindrical hole 114 and communicates with it. The second cylindrical hole 115 is concentric with the first cylindrical hole 114, and the diameter of the second cylindrical hole 115 is larger than the diameter of the first cylindrical hole 114. A third cylindrical hole 116 is provided at the lower end of the second cylindrical hole 115 and communicates with it. The diameter of the third cylindrical hole 116 is larger than the diameter of the second cylindrical hole 115, and the third cylindrical hole 116 communicates with the bottom end of the sensor housing 11.

[0083] A second slot 117 is provided at the junction of the first connecting hole 113 and the first cylindrical hole 114. The second slot 117 is inclined at 45°, specifically, the second slot 117 is inclined at 45° toward the inner side of the sensor housing 11. Preferably, the second slot 117 is a rectangular hole, and the projections of the first connecting hole 113 and the first cylindrical hole 114 toward the second slot 117 are part of the second slot 117.

[0084] It is worth noting that the second slot 117 is connected to the first side 118 of the sensor housing 11 to facilitate the placement of the dichroic mirror 14 disposed in the second slot 117.

[0085] In this embodiment, from the top end of the sensor housing 11, there are interconnected stepped holes 111, first slot holes 112, first connecting holes 113, second slot holes 117, first cylindrical holes 114, second cylindrical holes 115 and third cylindrical holes 116.

[0086] The sensor housing 11 also includes a third slot 119, a fourth slot 110, and a second connecting hole 120. The third slot 119 is located on the second side surface 121 of the sensor housing 11, which is perpendicular to the first side surface 118. One end of the third slot 119 communicates with the second side surface 121, and the other end communicates with one end of the second connecting hole 120. The other end of the second connecting hole 120 communicates with the first cylindrical hole 114, and the centerline of the second connecting hole 120 is perpendicular to the axis of the first cylindrical hole 114. The projection of the second connecting hole 120 toward the third slot 119 is a portion of the end face of the third slot 119. The fourth slot 110 communicates perpendicularly with the second connecting hole 120 and is located in the middle of the second connecting hole 120. The second connecting hole 120 passes through the center of the fourth slot 110. Preferably, the third slot 119 and the second connecting hole 120 are both cylindrical holes and are concentric, and the diameter of the second connecting hole 120 is smaller than the diameter of the third slot 119.

[0087] It is worth noting that the fourth slot 110 is connected to the first side 118 to facilitate the placement of the second filter 17. To further facilitate the placement of the filter, the lower end of the fourth slot 110 is provided with a strip hole 122 perpendicular to the fourth slot 110, and the fourth slot 110 is connected to the middle of the strip hole 122.

[0088] For the connection of wiring between internal components of the sensor housing 11, a wire groove 123 is also provided inside the sensor housing 11. The wire groove 123 is located on the second side 121 and is used to place the wires of the electrical components inside the sensor housing 11. The wire groove 123 is located above the third slot 119, and the upper end of the wire groove 123 communicates with the stepped hole 111, while the lower end of the wire groove 123 communicates with the third slot 119.

[0089] A battery slot 124 is provided below the third slot 119, and the battery slot 124 is connected to the third slot 119 and the wire slot 123.

[0090] In this embodiment, the wire groove 123, the third slot 119 and the battery slot 124 are located on the second side 121 and pass through the second side 121.

[0091] A first mounting groove 125 is provided at the lower end of the first side surface 118. The first mounting groove 125 communicates with the first slot 112, the second slot 117, the fourth slot 110, and the wire groove 123. To create a light-proof environment inside the sensor housing 11, a side cover plate 126 is provided on the first mounting groove 125. The first mounting groove 125 has a threaded hole for connecting with the side cover plate 126. Preferably, the first mounting groove 125 is a rectangular groove and communicates with the third side surface 127 of the sensor housing 11, which is parallel to the second side surface 121.

[0092] A second mounting slot 128 is provided at the top of the sensor housing 11, and the bottom of the second mounting slot 128 communicates with the stepped hole 111 and the wire groove 123. Preferably, the second mounting slot 128 is a rectangular slot, and the second mounting slot 128 communicates with the first side 118, the opposite side of the first side 118, and the third side 127.

[0093] In order to create a light-proof environment inside the sensor housing 11, a top cover plate 129 is provided on the second mounting slot 128, and a threaded hole is provided on the second mounting slot 128 for connecting with the top cover plate 129.

[0094] In this embodiment, viewed from the outside of the sensor housing 11, the sensor housing 11 includes a first mounting portion 130, a second mounting portion 131, a third mounting portion 132, and a fourth mounting portion 133. Preferably, the first mounting portion 130, the second mounting portion 131, the third mounting portion 132, and the fourth mounting portion 133 are all rectangular blocks. The first mounting portion 130, the second mounting portion 131, and the third mounting portion 132 are disposed on one side of the fourth mounting portion 133, and the other side of the fourth mounting portion 133 is the second side surface 121.

[0095] Specifically, one side of the first mounting portion 130 and the second mounting portion 131 is directly connected to one side of the fourth mounting portion 133. The other side of the first mounting portion 130 is a third side surface 127. The other side of the second mounting portion 131 is connected to the third mounting portion 132. The second mounting portion 131 and the third mounting portion 132 have the same height. The first mounting portion 130 is located above the second mounting portion 131 and the third mounting portion 132, and the sum of the heights of the first mounting portion 130 and the second mounting portion 131 is equal to the height of the fourth mounting portion 133. The width of the second mounting portion 131 is equal to the width of the fourth mounting portion 133. The width of the first mounting portion 130 is less than the width of the second mounting portion 131 but greater than the width of the third mounting portion 132. Preferably, the first mounting portion 130 is located in the middle of the fourth mounting portion 133, and the third mounting portion 132 is located in the middle of the second mounting portion 131.

[0096] In this embodiment, the stepped hole 111, the first slot 112, the first connecting hole 113, the second slot 117, the first cylindrical hole 114, the third slot 119, the second connecting hole 120, the fourth slot 110, and the wire groove 123 are concentrated in the first mounting part 130, the battery slot 124 is located in the second mounting part 131, the third cylindrical hole 116 is located in the third mounting part 132, and one end of the second cylindrical hole 115 is located in the first mounting part 130, and the other end is located in the third mounting part 132.

[0097] The wireless sensor 1 also includes a light source 12, a first filter 13, a dichroic mirror 14, a self-focusing lens 15, a sleeve 16, a second filter 17, a photoelectric conversion element 18, and a second mainboard 19. The first battery 10, the light source 12 is disposed in the stepped hole 111, the first filter 13 is disposed in the first slot 112, the dichroic mirror 14 is disposed in the second slot 117, the self-focusing lens 15 is disposed in the first cylindrical hole 114 and the second cylindrical hole 115, and the lower end of the self-focusing lens 15 is located in the sleeve 16, the diameter of the lower end of the self-focusing lens 15 is equal to the inner diameter of the sleeve 16, the sleeve 16 is disposed in the third cylindrical hole 116, the two diameters are equal, and the length of the sleeve 16 is equal to the length of the third cylindrical hole 116, the second filter 17 is disposed in the fourth slot 110, the photoelectric conversion element 18 is disposed in the third slot 119, the second main board 19 is disposed on the second side 121 and covers the second side 121, and the first battery 10 is disposed in the battery slot 124. More preferably, the third slot 119 can be omitted in the sensor housing 11, so that the photoelectric conversion element 18 can be directly disposed on the second side 121 through the second motherboard 19.

[0098] Optionally, the light source 12 can be an LED light source or a semiconductor laser diode, such as a vertical-cavity surface-emitting laser (VCSEL). The photoelectric conversion element 18 can be a photodiode.

[0099] More preferably, the first filter 13 has both filtering and optical path collimation effects, and its specific structure may include a base surface. One side of the base surface is a smooth surface, and the other side is an embossed surface. More preferably, the smooth surface is located closer to the light source 12. The embossed surface is used to collimate the light emitted by the light source 12. The scattered incident light can be collimated in the optical path after passing through the embossed surface, further reducing light loss and increasing the light intensity entering the animal's brain, thus greatly improving the experimental results. Preferably, a filter film is provided on the smooth surface, and the filter film is used to filter stray light.

[0100] Furthermore, in a wireless fiber optic recording system, the first filter 13 may not contain a filter film, but only an embossed surface to collimate the light emitted by the light source 12.

[0101] Furthermore, the second filter 17 can also have the same structure as the first filter 13, also having a smooth surface and an embossed surface. Preferably, the smooth surface is positioned close to the dichroic mirror 14, and the embossed surface is positioned close to the photocell. More preferably, a filter film is disposed on the smooth surface, which is used to filter stray light, and the embossed surface is used to focus fluorescent light, so that the fluorescence fed back to the photoelectric conversion element 18 is more concentrated, thereby obtaining a high-quality and high-efficiency optical signal.

[0102] Furthermore, since the light path changes when it passes through the relief surface, it is preferable that the smooth surface is the entrance surface of the incident light and the relief surface is the exit surface of the incident light, that is, the light first passes through the filter film on the smooth surface and then passes through the relief surface for collimation.

[0103] It should be noted that the surface structure design of the embossed surfaces of the first filter 13 and the second filter 17 is based on the design principle of surface-embossed micro / nano devices for collimation:

[0104] Based on the position and wavelength λ of the light source, as well as the divergence angle θ of the diverging spherical wave, the phase distribution of the light field propagating from the light source onto the front surface (i.e., the smooth surface) of the surface-embossed micro / nano device can be calculated. In this design, the target light field is a collimated plane wave, meaning the light field on the rear surface (i.e., the relief surface) of the surface-embossed micro / nano device is a collimated plane wave, and its phase distribution can be denoted as... Therefore, the phase distribution of the designed device for:

[0105]

[0106] Here, mod represents the modulo operation.

[0107] Using phase The relationship between the surface relief function h(x,y) and the surface relief function h(x,y) is calculated as follows:

[0108]

[0109] Where λ is the wavelength of the light source and n is the refractive index of the surface-embossed micro / nano device material.

[0110] Once the surface relief function h(x,y) of the device is obtained, a surface relief micro-nano device that meets the requirements can be fabricated using appropriate micro-nano fabrication techniques.

[0111] With the arrangement of the aforementioned optoelectronic components, within the sensor housing 11, the light source 12 emits an excitation light source, causing the neurons in the brain region of the animal that has undergone viral expression to produce a fluorescent reaction. The fluorescence is reflected back to the wireless sensor 1, where it is received by the photoelectric conversion element 18, converting the light signal into an electrical signal. The second motherboard 19 is equipped with a wireless Bluetooth module, enabling wireless communication with the wireless Bluetooth module on the DAQ host 2.

[0112] The optical path in this embodiment is as follows: the light source 12, the first filter 13, the dichroic mirror 14, the coupling lens (the self-focusing lens 15 in this embodiment), and the single-mode optical fiber 32 are arranged along the first axis; the photoelectric conversion element 18 and the second filter 17 are arranged along the second axis. The first axis is perpendicular to the second axis, and the second axis passes through the dichroic mirror 14. The first filter 13 has a transmission bandwidth of 40 nm, an od value of at least 4 (preferably 5), and a wavelength range of 430 nm to 510 nm (preferably 450 nm to 490 nm); the second filter 17 has a transmission bandwidth of 40 nm, an od value of at least 4 (preferably 5), and a wavelength range of 505 nm to 545 nm.

[0113] The dichroic mirror 14 is tilted at a 45° angle, and its function is to reflect fluorescence incident at a 45° angle and transmit light sources incident at a 45° angle. In use, the dichroic mirror 14 is set at a 45° angle to the first axis and the second axis.

[0114] Furthermore, the excitation optical path is transmitted along the first axis, and the excitation optical path includes a first optical path and a second optical path, the second optical path having an off-center angle relative to the first optical path. The fluorescence is transmitted to the photoelectric conversion element 18 along the second optical path and the second axis.

[0115] In this embodiment, after the light source 12 emits light, it is filtered by the first filter 13 and reaches the dichroic mirror 14. The light is then transmitted through the dichroic mirror 14, and after passing through the coupling lens, reaches the single-mode fiber 32. External fluorescence enters from the single-mode fiber 32, is transmitted through the coupling lens, reaches the dichroic mirror 14, is reflected by the dichroic mirror 14 onto the second axis, and after being filtered by the second filter 17, reaches the photoelectric conversion element 18. The photoelectric conversion element 18 converts the received optical signal into an electrical signal. Furthermore, the first filter 13 can be omitted, making the optical path more streamlined compared to existing technologies and minimizing the impact on light; at the same time, it makes the wireless sensor lighter and smaller. In this structure, the excitation light of the light source 12 will directly pass through the dichroic mirror 14 (eliminating the influence of the first filter 13 on light intensity, etc.), thereby enhancing the excitation light intensity. The reflected fluorescence first passes through the second filter 17 to filter out useful light information, and then the light information is refracted into the photodiode 18 by the refraction of the dichroic mirror 14.

[0116] The ferrule 3 includes a ceramic head 31 and a single-mode optical fiber 32. One end of the single-mode optical fiber 32 is implanted into the skull of the small animal 100, and the other end is located inside the ceramic head 31. One end of the ceramic head 31 is fixed to the head of the small animal 100 outside the skull with dental cement 200, and the other end is located inside the cannula 16.

[0117] To facilitate the clamping and fixation of the insert pin 3, a circumferential groove 33 is provided at the lower end of the ceramic head 31, which facilitates clamping with tweezers and fixation with dental cement 200. The circumferential groove 33 can be circular, cylindrical, or other shapes, but is preferably circular.

[0118] A through hole is provided along the axis of the ceramic head 31. One end of the single-mode optical fiber 32 is disposed in the through hole, and the end of the single-mode optical fiber 32 is flush with the end of the ceramic head 31. The single-mode optical fiber 32 is fixedly connected to the ceramic head 31. Exemplarily, the single-mode optical fiber 32 is connected to the through hole by adhesive. Since the light transmittance of a white ceramic head is not high, in this embodiment, the ceramic head 31 is black.

[0119] In this embodiment, the installation steps of the ceramic head 31 and the single-mode optical fiber 32 are as follows: First, the single-mode optical fiber 32 is cut to the required length using an optical fiber cleaver; second, the single-mode optical fiber 32 is coated with optical adhesive and inserted into the through hole of the ceramic head 31, with the end of the single-mode optical fiber 32 away from the circumferential groove 33 flush with the end of the ceramic head 31; finally, it is cured using ultraviolet light.

[0120] It is worth noting that in this embodiment, the ceramic head 31 has two diameter specifications, namely 2.5mm and 1.25mm, and the inner diameter of the sleeve 16 needs to be modified accordingly.

[0121] The host computer controls the DAQ host 2 to pair with the wireless sensor 1 via Bluetooth, enabling simultaneous control of two wireless sensors 1. It can control the on / off state of the wireless sensors 1, the on / off state of the light source 12, and adjust brightness, among other things. The host computer can also connect to an external behavioral camera, displaying and saving the camera images. Both the video and the corresponding light intensity records can be saved, providing richer data for experiments.

[0122] The wireless fiber optic recording system also includes a charging module 4, which is used to charge the wireless sensor 1 and also has the function of storing the wireless sensor 1. The charging module 4 specifically includes an upper cover 41 and a lower housing 42, with the upper cover 41 covering the lower housing 42.

[0123] The upper cover 41 can be transparent, semi-transparent, or opaque. Preferably, the upper cover 41 is transparent so that the charging status of the wireless sensor 1 can be clearly seen. In this embodiment, the upper cover 41 can be a cylindrical shell or a rectangular shell; preferably, the upper cover 41 is a rectangular shell. The shape of the lower housing 42 is adapted to the shape of the upper cover 41.

[0124] The lower box body 42 includes a lower box body top cover 421, a lower box body 422, and a lower box body bottom cover 423. The lower box body top cover 421 covers the upper part of the lower box body 422, and the lower box body bottom cover 423 covers the lower part of the lower box body 422. The lower box body top cover 421 and the lower box body 422 can be detachable or integrally formed. The side wall of the lower box body top cover 421 is recessed relative to the side wall of the lower box body 422 by a certain distance, which is equal to the wall thickness of the top cover 41, so that the top cover 41 fits precisely on the lower box body 422.

[0125] The lower housing top cover 421 has a rectangular structure. A sensor receiving slot 4211 is located at the top of the lower housing top cover 421. The wireless sensor 1 is disposed within the sensor receiving slot 4211. There are two sensor receiving slots 4211, capable of simultaneously accommodating two wireless sensors 1 and charging the wireless sensors 1 when needed. The sensor receiving slot 4211 is a rectangular slot, and multiple charging spring holes 4212 are provided within the sensor receiving slot 4211, preferably three.

[0126] The top of the lower housing cover 421 is also provided with a wake-up switch hole 4213 and a charging indicator light hole 4214. There are two of each type, and they are aligned with two sensor receiving slots 4211. Specifically, the sensor receiving slots 4211 are located between the wake-up switch hole 4213 and the charging indicator light hole 4214, with one wake-up switch hole 4213, one sensor receiving slot 4211, and one charging indicator light hole 4214 located in the same row. In this embodiment, the wake-up switch hole 4213, the sensor receiving slot 4211, and the charging indicator light hole 4214 are generally located in the middle part of the top of the lower housing cover 421.

[0127] When the lower box top cover 421 is detachably connected to the lower box body 422, the bottom of the lower box top cover 421 is provided with a protrusion 4215 that is recessed relative to its edge. The protrusion 4215 cooperates with the inner wall of the lower box body 422 and is used to be stuck in the lower box body 422.

[0128] When the lower housing body 422 and the lower housing bottom cover 423 are detachably connected, the side wall of the lower housing bottom cover 423 is recessed relative to the side wall of the lower housing body 422 by a certain distance, which is less than the wall thickness of the lower housing body 422. In this embodiment, the lower housing body 422 and the lower housing bottom cover 423 are connected by screws. The screws are preferably round-headed screws, so that the round head of the screw protrudes outside the lower housing bottom cover 423 to support the charging module. Furthermore, a rubber pad can be provided outside the round head to reduce wear on the bottom surface of the lower housing bottom cover 423.

[0129] The lower housing body 422 has a receiving cavity 4221, and a USB charging port 4222 is provided on the side wall of the lower housing body 422, the USB charging port 4222 communicating with the receiving cavity 4221. Specifically, the USB charging port 4222 is a stepped hole, and a chamfer is provided on the outer wall surface of the lower housing body 422. Preferably, the USB charging port 4222 is a rectangular hole.

[0130] The charging module 4 also includes a third motherboard 43 and a second battery 44, both of which are located within the receiving cavity 4221, with the second battery 44 positioned below the third motherboard 43. The third motherboard 43 is equipped with a wake-up switch 431 and a charging indicator light 432, which correspond to the wake-up switch hole 4213 and the charging indicator light hole 4214, respectively. When the third motherboard 43 is installed within the lower housing body 422, the upper ends of both the wake-up switch 431 and the charging indicator light 432 slightly protrude from the top surface of the lower housing cover 421 for operation and observation. The wake-up switch 431 is used to activate the working mode of the wireless sensor 1, and the charging indicator light 432 indicates the battery level or charging status of the wireless sensor 1.

[0131] The third motherboard 43 is also provided with charging spring pins 433. Multiple charging spring pins 433 are grouped together. In this embodiment, there are two groups of charging spring pins, which correspond to the two sensor receiving slots 4211. Correspondingly, the wireless sensor 1 is provided with multiple charging contacts, preferably three, which are located on the third motherboard 43. The charging contacts of the wireless sensor 1 contact the charging spring pins 433, and gravity presses the charging spring pins down to achieve electrical connection between the two, thereby realizing charging.

[0132] The third motherboard 43 has a USB interface 434 on one side, which corresponds to the USB charging port 4222. The end of the USB interface 434 is partially disposed in the USB charging port 4222.

[0133] The two wireless sensors 1 can be used cyclically, with the other used for recording when one loses power. The single-mode optical fiber 32 portion of the ferrule 3 is implanted inside the brain of the small animal 100, and the exposed ceramic head 31 is fixed to the brain with dental cement 200. The wireless sensor 1 and the ferrule 3 are detachably connected (e.g., by interference fit, threaded connection, etc.). When one wireless sensor 1 loses power, it is removed and placed in the wireless sensor charger for charging, then replaced with the other wireless sensor 1 for continued use.

[0134] The circumferential groove 33 of the insert pin 3 can facilitate the gripping of the insert pin with tweezers, and can also allow dental cement 200 to enter the circumferential groove during fixation, so that the insert pin 3 is firmly fixed.

[0135] The photoelectric conversion element 18 in the wireless sensor 1 converts the optical signal into an electrical signal, and then transmits the electrical signal to the DAQ host 2 via Bluetooth. During the transmission process, if the first battery 10 suddenly loses power, the previously recorded data can still be saved.

[0136] In this embodiment, the fiber optic patch cord (including extension cord and slip ring) is eliminated. The wireless sensor is directly connected to the ferrule, and communication and control are achieved via a wireless Bluetooth module. This eliminates the original structure that transmitted light via fiber optic patch cords. The optical structure is miniaturized and placed within a small wireless sensor on the mouse's head, avoiding transmission interference caused by the original patch cords. Calcium imaging signals can be directly acquired from the nearby wireless sensor, resulting in more accurate data. Furthermore, since patch cords are no longer needed, the adverse effects of restricting mouse behavior caused by patch cords are eliminated.

[0137] Example 2

[0138] Another specific embodiment of the present invention, such as Figures 1 to 28 As shown, a wireless optical fiber recording system is disclosed. The system differs from Embodiment 1 in the following ways in structure, while the other structures and corresponding beneficial effects are the same as in Embodiment 1, and will not be described in detail here.

[0139] Specifically, the lower housing top cover 421 is provided with battery level indicator light holes 4216. Multiple battery level indicator light holes 4216 are arranged side-by-side between the two wake-up switch holes 4213. The third main board 43 is provided with battery level indicator lights 435, which correspond to the battery level indicator light holes 4216. When the third main board 43 is installed in the receiving cavity 4221, the battery level indicator lights 435 are located within the battery level indicator light holes 4216. The battery level indicator lights 435 are used to indicate the battery level of the charging module 4 itself. When the charging module 4 has insufficient power, it needs to be charged via the USB port.

[0140] The lower box top cover 421 is also provided with a limiting body 424. The limiting body 424 is located between the two sensor receiving slots 4211 and is used to limit the wireless sensor 1 located in the sensor receiving slot 4211, further ensuring the reliability of the connection between the wireless sensor 1 and the charging spring pin 433, and at the same time ensuring the stability of the wireless sensor 1 stored in the sensor receiving slot 4211.

[0141] The limiting body 424 includes a limiting post 4241, a rotating shaft 4242, and a pressure cover 4243. The limiting post 4241 is disposed between the two sensor receiving slots 4211. The rotating shaft 4242 is disposed at one end of the limiting post 4241, preferably at the end away from the charging indicator hole 4214. The pressure cover 4243 is rotatably connected to the rotating shaft 4242.

[0142] Furthermore, one end of the limiting post 4241 is provided with a pivot mounting hole 42411, the axis of which is parallel to the top cover 421 of the lower box, and the pivot mounting hole 42411 extends through both sides of the limiting post 4241. The end of the limiting post 4241 is provided with a T-shaped hole 42412, which communicates with the pivot mounting hole 42411 and connects the end face and top surface of the limiting post 4241. The axis of symmetry of the T-shaped hole 42412 is parallel to the top cover 421 of the lower box.

[0143] To prevent the pressure cap 4243 from interfering with the limiting post 4241 during the flipping process, the top surface of the limiting post 4241 near the T-shaped hole 42412 is lower than the top surface of the limiting post 4241 near the charging indicator hole 4214.

[0144] The bottom surface of the pressure cap 4243 is provided with a connecting block 42431. The end of the connecting block 42431 is provided with a hole that mates with the rotating shaft 4242. In order to avoid interference between the pressure cap 4243 and the T-shaped hole 42412 during the flipping process, the length and width of the connecting block 42431 are smaller than the corresponding size of the T-shaped hole 42412, and the end of the connecting block 42431 is rounded.

[0145] The top surface of the pressure cap 4243 is provided with a top plate receiving groove 42432, and the bottom of the top plate receiving groove 42432 is provided with a first magnetic receiving groove 42433. A first magnetic element 42434 is provided in the first magnetic receiving groove 42433, and a pressure cap top plate 42435 is provided in the top plate receiving groove 42432. The pressure cap top plate 42435 fixes the first magnetic element 42434 in the pressure cap 4243. Preferably, the top plate receiving groove 42432 is a rectangular groove, and the first magnetic receiving groove 42433 is a circular groove. It should be noted that the first magnetic receiving groove 42433 avoids being directly above the connecting block 42431.

[0146] The limiting post 4241 is provided with a second magnetic attraction groove 42413, which is located below the first magnetic attraction groove 42433. A second magnetic attraction element 42414 is provided within the second magnetic attraction groove 42413. The first magnetic attraction element 42434 and the second magnetic attraction element 42414 can attract each other, so that the pressure cap 4243 can remain fixed when it is placed on the limiting post 4241. It should be noted that both the first magnetic attraction element 42434 and the second magnetic attraction element 42414 can be magnets, or one can be a magnet and the other can be a component that can be attracted by a magnet. Preferably, both the first magnetic attraction element 42434 and the second magnetic attraction element 42414 are magnets.

[0147] It is worth noting that the height of the limiting body 424 should meet the following requirements: when the wireless sensor 1 is placed in the sensor receiving slot 4211, the height of the cover 4243 should be approximately the same as the height when the cover is in a horizontal state, so as to ensure that the wireless sensor 1 will not come out during charging and transportation, and that the charging contacts of the wireless sensor 1 make good contact with the charging spring pin 433.

[0148] To prevent the pressure cap 4243 from damaging the wireless sensor 1 when pressed down, and to increase the friction between the wireless sensor 2 and the pressure cap 1243 so that a smaller downward pressure can stably position the wireless sensor 2 in the sensor receiving groove 1211, the bottom of the pressure cap 4243 is provided with an elastic element 42436. Preferably, the elastic element 42436 is a rubber pad. In this embodiment, there are three elastic elements 42436, two of which correspond to the third side surface 127 of the two wireless sensors 1 respectively, and one corresponds to the top of the limiting post 4241. The top surface of the limiting post 4241 is not higher than the third side surface 127 of the wireless sensor 1, so that the pressure cap 4243 can press down on the wireless sensor 1.

[0149] This invention employs wireless transmission and control, eliminating the need for cable connections between the wireless sensors and the DAQ host. This not only improves system convenience and reduces interference with animal behavior, allowing small animals to move freely during experiments and effectively reducing activity restrictions, but also offers inherent advantages in enclosed experimental settings. It enables researchers to conduct pipeline-based behavioral experiments. Furthermore, by eliminating fiber optic patch cords, it improves light transmission efficiency within the system, reduces interference in signal recording, and enhances recording accuracy, resulting in more objective experimental results.

[0150] This invention moves the complex optical structure from the original DAQ host to a more forward-facing wireless sensor, directly acting on the brain of a small animal (mouse). It is small and lightweight (weight ≤ 3g, volume ≤ 2 cubic centimeters) and will not affect the mouse's activity. The wireless sensor achieves an accuracy of 1uW (optical power). Through precise optical path design, it ensures the incident light of the required wavelength while reflecting other wavelengths, thus improving the sensor's sensitivity.

[0151] This invention adds Bluetooth transmission, enabling wireless sensor control and data reception via Bluetooth, thus achieving wireless transmission and eliminating the need for fiber optic patch cords. The original bulky recording host, which housed a light source system, optical sensors, and data transmission capabilities, was quite large. The DAQ host of this invention eliminates the optical structure, resulting in a lighter and more compact host. Therefore, the DAQ host can be made smaller, primarily for data interaction, receiving data from the wireless sensors and sending it to the host computer. Its function is now more singular, enabling more accurate and faster data processing and transmission.

[0152] The wireless sensor of this invention is active, containing a battery with a battery life of at least one hour, and can be recharged via a charging module after use. Patch cords themselves exhibit fluorescence; when light travels to the optical fiber, the fluorescence of the fiber itself causes interference. To avoid this interference, existing technologies add a reference light, which complicates the optical path structure, increases the size of the system, and complicates subsequent data processing. However, the wireless optical fiber recording system of this invention does not require patch cords, thus avoiding this interference and resulting in more accurate results.

[0153] Those skilled in the art will understand that all or part of the processes of the methods described in the above embodiments can be implemented by a computer program instructing related hardware, and the program can be stored in a computer-readable storage medium. The computer-readable storage medium may be a disk, optical disk, read-only memory, or random access memory, etc.

[0154] The above description is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any changes or substitutions that can be easily conceived by those skilled in the art within the scope of the technology disclosed in the present invention should be included within the scope of protection of the present invention.

Claims

1. A wireless optical recording system, characterized by, The application relates to a wireless sensor data acquisition system for small animals, which comprises an upper computer, a wireless sensor, a DAQ host, a plug-in pin and a charging module. The wireless sensor comprises a sensor shell, a light source, a dichroic mirror, a self-focusing lens, a second filter and a photoelectric conversion element arranged in the sensor shell; the light source, the dichroic mirror and the self-focusing lens are arranged along a first axis, the photoelectric conversion element and the second filter are arranged along a second axis, the first axis is perpendicular to the second axis, the second axis passes through the dichroic mirror, and the dichroic mirror is arranged at an angle of 45 degrees with respect to the first axis and the second axis. The charging module provides power and storage space for the wireless sensor, and comprises a lower box body, a lower box body top cover, a sensor accommodating groove and a limiting body arranged on the lower box body top cover, a limiting column arranged between the two sensor accommodating grooves, a rotating shaft arranged at one end of the limiting column, a gland rotatably connected with the rotating shaft, a top plate accommodating groove arranged on the top surface of the gland, a first magnetic attraction accommodating groove arranged at the bottom of the top plate accommodating groove, a first magnetic attraction element arranged in the first magnetic attraction accommodating groove, a second magnetic attraction accommodating groove arranged in the limiting column, and a second magnetic attraction element arranged in the second magnetic attraction accommodating groove.

2. The wireless optical recording system of claim 1, wherein, The wireless sensor further comprises a first filter, which comprises a base surface, one side of the base surface being a smooth surface and the other side being a relief surface.

3. The wireless optical recording system of claim 1, wherein, The plug-in pin comprises a ceramic head and a single-mode optical fiber, one end of the single-mode optical fiber being implanted in the intracranial cavity of a small animal, the other end being arranged in the ceramic head, one end of the ceramic head being fixed to the head of the small animal, and the other end being arranged in the wireless sensor.

4. The wireless optical recording system of claim 3, wherein, The single-mode optical fiber is arranged along the first axis.

5. The wireless optical recording system of claim 3, wherein, The lower end of the ceramic head is provided with a circumferential groove.

6. The wireless optical recording system of any of claims 1-4, wherein, The DAQ host is connected with and controls the wireless sensor through a wireless module, and can simultaneously control at least two wireless sensors.

7. The wireless optical recording system of any of claims 1-4, wherein, The upper computer is used for controlling the wireless sensor and / or the DAQ host, and collecting, storing, analyzing, inputting and outputting fiber recording data.

Images