An in vivo observation bath for animal mesenteric microcirculation

Abstract

This invention discloses a live observation bath for animal mesenteric microcirculation, comprising a water bath tank (1) with an open upper part, and a microcirculation observation ring (2) disposed in the water bath tank (1). The microcirculation observation ring (2) is installed in the water bath tank (1) by an observation ring support frame (4); the upper surface of the microcirculation observation ring (2) is an upwardly convex arc surface. Using the above technical solution, the temperature and humidity of the experimental environment are kept constant; the perfusion fluid will not remain on the observation surface and affect observation; the adjustment of the experimental process is more convenient, and different visual effects can be obtained as needed, including adjustment of the field of view, focal length, and framing range.

Description

Technical Field

[0001] This invention belongs to the technical field of medical experimental instruments and equipment. More specifically, this invention relates to a live observation bath for the microcirculation of the animal mesentery. Background Technology

[0002] The mesenteric microcirculation in animals serves as a crucial window for microcirculation observation and research. This observation and study is of significant value in medical education and scientific research, particularly in the field of shock microcirculation research. Animal mesenteric microcirculation observation devices are essential instruments for microcirculation observation and research, and have wide applications in medical education and scientific research, specifically in the field of shock microcirculation research.

[0003] In existing technologies, in vivo observation experiments of animal mesenteric microcirculation use a constant temperature water bath to keep the experimental subjects warm, so that the experimental subjects are kept in a constant temperature and humidity state to simulate the microcirculation environment of animal mesentery in a living state; generally, a temperature control probe (temperature sensor) is used to collect the temperature signal in the water bath, and the experimental device is heated by a heating rod.

[0004] The disadvantages of existing technology are:

[0005] 1. The device is complex and prone to failure, damage and danger: The microcirculation perfusion fluid is an electrolyte, and the constant temperature thermocouple device is immersed in the perfusion fluid. When energized, it is very easy to fail, cause damage to the equipment, and even pose a risk of electric shock.

[0006] 2. Uneven heating: Insufficient circulation of the perfusion fluid in the microcirculation observation box causes the temperature control probe to only detect the local temperature around the probe. In addition, the mesentery covers the temperature control probe, making the local temperature around it higher, while the exposed areas are colder. As a result, the temperature of the intestinal loops suspended on the perfusion fluid surface is much lower than that of the intestinal loops below the fluid surface, especially when the room temperature is low. If the temperature control probe and heating rod are exposed on the perfusion fluid surface, the temperature will continue to rise and may even burn out the instrument.

[0007] 3. The method of observation affects the experimental results: When the room temperature is low, the water vapor in the perfusion fluid of the microcirculation box can also blur the lens of the microscopic observation system, affecting the observation. Summary of the Invention

[0008] This invention provides an in vivo observation bath for animal mesenteric microcirculation, the purpose of which is to improve the effect of microcirculation observation experiments.

[0009] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0010] The present invention provides an in vivo observation bath for the microcirculation of the animal mesentery, comprising a water bath tank body with an open upper part; a microcirculation observation ring is disposed in the water bath tank body, and the microcirculation observation ring is mounted in the water bath tank body by an observation ring support frame; the upper surface of the microcirculation observation ring is an upwardly convex arc surface.

[0011] The microcirculation observation ring is made of transparent material, and an illumination source is set inside the microcirculation observation ring.

[0012] The lighting source is an LED light source.

[0013] The water bath tank is made of a material with thermosensitive temperature control properties.

[0014] The observation ring support frame is equipped with an observation ring lifting mechanism.

[0015] The observation ring lifting mechanism employs a screw drive mechanism. A nut is mounted on the microcirculation observation ring, and a vertically upward screw is mounted on the bottom surface of the water bath tank. The nut and screw are threaded together. A horizontally arranged lifting adjustment transmission rod is mounted on the water bath tank. A driven bevel gear is mounted on the screw, and a driving bevel gear is mounted at the inner end of the lifting adjustment transmission rod. The driving bevel gear meshes with the driven bevel gear. A lifting adjustment knob is mounted on the end of the lifting adjustment transmission rod extending out of the water bath tank.

[0016] The observation bath is equipped with a camera; the camera is mounted on a horizontal connecting rod, which is mounted on a camera support column; and a column base is provided at the bottom of the camera support column.

[0017] A camera light is provided on the side of the camera.

[0018] The observation bath is equipped with an observation device control host; the observation device control host is connected to the camera via a signal line.

[0019] The observation bath is equipped with a display, which is an interactive device for human-computer dialogue.

[0020] The technical effects achieved by adopting the above technical solution are as follows:

[0021] 1. The entire microcirculation box is made of thermosensitive temperature control material, eliminating the need to heat and maintain the temperature of the water in the constant temperature water bath, thus keeping the temperature and humidity of the experimental environment constant.

[0022] 2. The surface of the microcirculation observation ring is a raised arc surface with very small curvature, allowing the perfusion fluid to flow out and not remain on the observation surface, thus not affecting the observation;

[0023] 3. The microcirculation observation ring has a lifting function, which can be easily adjusted according to the needs. Raising the observation ring can lower the Tyrode's liquid level in the bath, which is beneficial for the fixation of mesenteric microcirculation. Lowering the observation ring can raise the Tyrode's liquid level in the bath, which is beneficial for mesenteric microcirculation perfusion.

[0024] 4. The observation ring uses LED lighting, which has the advantages of strong, focused, and cool light source;

[0025] 5. The experimental process is more convenient to adjust, and different visual effects can be obtained as needed, including the adjustment of field of view, focal length, and framing range;

[0026] 6. The observation results are displayed on the screen, making observation convenient; it also facilitates the analysis and storage of the observation results in conjunction with the system's analysis software. Attached Figure Description

[0027] The following is a brief explanation of the contents shown in the attached figure and the markings therein:

[0028] Figure 1 This is a schematic diagram of the microcirculation observation tank structure of the present invention;

[0029] Figure 2 for Figure 1 A top view of the structure shown;

[0030] Figure 3 A top view of the structure of the animal experimental table and bath;

[0031] Figure 4 This is a schematic diagram of the overall system structure of the present invention;

[0032] Figure 5 This is a schematic diagram of the bottom installation structure of the water bath tank of the present invention.

[0033] The diagram is marked as follows:

[0034] 1. Water bath tank body; 2. Microcirculation observation ring; 3. LED light source; 4. Observation ring support frame; 5. Observation ring lifting mechanism; 6. Lifting adjustment transmission rod; 7. Lifting adjustment knob; 8. Camera; 9. Camera lighting; 10. Camera support column; 11. Camera horizontal connecting rod; 12. Column base; 13. Signal cable; 14. Monitor; 15. Observation device control host; 16. Camera locking knob; 17. Power supply; 18. Animal experimental table; 19. Fixing clamp; 20. Fixing screw. Detailed Implementation

[0035] The specific embodiments of the present invention will be further described in detail below with reference to the accompanying drawings, so as to help those skilled in the art to have a more complete, accurate and in-depth understanding of the inventive concept and technical solution of the present invention.

[0036] like Figures 1-4 The structure of the present invention is a live observation bath for animal mesenteric microcirculation, including a water bath tank 1 with an open upper part.

[0037] The water bath tank 1 (or box) can be scaled up or down according to the size of different animals and can be installed on different animal experimental tables 18; the microcirculation observation water bath can be installed after other experimental operations are completed to carry out microcirculation observation experimental operations, which makes the experiment more convenient.

[0038] To address the problems and overcome the shortcomings of existing technologies and achieve the goal of improving the effectiveness of microcirculation observation experiments, the technical solution adopted by this invention is as follows:

[0039] like Figure 1 , Figure 2 As shown, the animal mesenteric microcirculation live observation bath of the present invention has a microcirculation observation ring 2 set in the water bath tank 1. The microcirculation observation ring 2 is installed in the water bath tank 1 by an observation ring support frame 4. The upper surface of the microcirculation observation ring 2 is an upwardly convex arc surface.

[0040] The difference and innovation of the improved animal mesenteric microcirculation observation device of this invention compared with existing microcirculation observation devices is that the upper surface of the microcirculation observation ring 2 is a raised arc surface with a very small curvature, on which the animal mesentery to be observed is placed. Because the raised arc surface allows the perfusion fluid to flow out slowly, it will not accumulate water on the observation surface and affect the observation. Excessive water accumulation will affect the condition of the animal mesentery.

[0041] Because the curvature of the raised arc surface is very small, almost flat, it has little impact on observation.

[0042] The microcirculation observation ring 2 is made of transparent material, and an illumination source is set inside the microcirculation observation ring 2.

[0043] Because the microcirculation observation ring 2 is made of transparent material and an illumination source is set inside the microcirculation observation ring 2, light can penetrate the animal's mesentery, allowing for a clearer observation of the dynamics of its microcirculation and obtaining better experimental results.

[0044] The lighting source is an LED light source 3. The LED light source 3 is connected to the power supply 17.

[0045] Using LED light sources, which are cold light sources, the method possesses the characteristics of being "strong," "focused," and "cool," effectively solving the technical challenge of simulating the intraperitoneal environment of in vitro mesenteric microcirculation. It provides sufficient illumination while avoiding excessively high temperatures generated by the lighting. The technical solution is reasonable, the experimental process is stable, and the operation is convenient. When used, it not only improves the observation effect of microcirculation but also objectively and truthfully reflects the observation results of microcirculation, making the observation and research of mesenteric microcirculation in teaching and scientific research experiments more ideal.

[0046] The LED light source 3 can be brightness adjusted to meet the lighting requirements of different experimental materials.

[0047] The water bath tank 1 is made of a material with thermosensitive constant temperature control properties.

[0048] The entire microcirculation box is made of thermosensitive temperature control material, eliminating the need to heat and maintain the temperature of the water in the constant temperature water bath, thus keeping the temperature and humidity of the experimental environment constant.

[0049] The observation ring support frame 4 is equipped with an observation ring lifting mechanism 5.

[0050] The microcirculation observation ring 2 has a lifting function, which can move up and down, and its observation height can be easily adjusted as needed to achieve different observation effects.

[0051] Adjusting the position of the microcirculation observation ring 2 according to the actual situation of microcirculation observation in animal experiments is beneficial for microscopic imaging (focusing). This not only facilitates microscopic observation of microcirculation, but also allows the microcirculation observation ring to be immersed in Tyrode's solution. The raising and lowering of the position of the microcirculation observation ring 2 can wet and perfuse the mesenteric microcirculation, and after long-term microcirculation observation, it can be lowered multiple times to immerse the mesenteric microcirculation in Tyrode's solution, providing multiple wet and perfuse treatments to the mesenteric microcirculation.

[0052] Moving the microcirculation observation ring 2 upwards can cause the Tyrode's fluid level to drop relatively, which is beneficial for fixing the field of view of mesenteric microcirculation; moving the microcirculation observation ring 2 downwards can cause the Tyrode's fluid level to rise relatively, which is beneficial for mesenteric wetting and perfusion. During long-term observation of mesenteric microcirculation, the ring can be moved downwards and upwards repeatedly to wet and perfuse the mesenteric microcirculation.

[0053] The observation ring lifting mechanism 5 adopts a screw drive mechanism, and its specific structure is as follows:

[0054] The spiral drive mechanism has a nut on the microcirculation observation ring 2 and a vertically upward screw on the bottom surface of the water bath tank 1. The nut and the screw are threaded together. A horizontally arranged lifting and adjusting transmission rod 6 is provided on the water bath tank 1. A driven bevel gear is provided on the screw, and a driving bevel gear is provided at the inner end of the lifting and adjusting transmission rod 6. The driving bevel gear meshes with the driven bevel gear. The lifting and adjusting transmission rod 6 extends out of the end of the water bath tank 1 and is provided with a lifting and adjusting knob 7.

[0055] The observation device is equipped with a camera 8. The observation device of the present invention uses the camera 8 to collect data on the dynamic effects of microcirculation in the animal mesentery, and then performs image analysis and processing.

[0056] The camera 8 is mounted on the camera horizontal connecting rod 11, the camera horizontal connecting rod 11 is mounted on the camera support column 10, and a column base 12 is provided at the bottom end of the camera support column 10.

[0057] At the end where the horizontal connecting rod 11 of the camera connects to the camera support column 10, a camera locking knob 16 is provided to fix the camera 8.

[0058] The horizontal connecting rod 11 of the camera can be rotated and adjusted on the camera support column 10. Its height can also be adjusted. After adjustment, it is secured using the camera locking knob 16.

[0059] A camera illumination light 9 is provided on the side of the camera 8.

[0060] The camera illumination lamp 9 illuminates the animal's mesentery from the front and outside, making the observed object clearer through the combined effect of internal and external light sources.

[0061] The camera illumination lamp 9 can be brightness adjusted to meet the lighting requirements of different experimental materials.

[0062] like Figure 4 As shown:

[0063] The observation device is equipped with an observation device control host 15; the observation device control host 15 is connected to the camera 8 via a signal line 13.

[0064] The observation device control host 15 is equipped with software for analyzing and processing images of in vivo observation of animal mesenteric microcirculation. It acquires microcirculation images of the animal mesentery through camera 8, and then performs real-time analysis and processing to obtain experimental results.

[0065] The observation device is equipped with a display 14, which is an interactive device for human-computer dialogue.

[0066] The display 14 uses a touch screen, which makes it convenient to set and control the experimental process and display the experimental results.

[0067] like Figure 5 As shown, the bottom of the water bath tank 1 of the present invention is provided with a fixing strap 19, which is used to fix the tank to the animal experimental table 18 and tightened with fixing screws 20. The fixing strap 19 can be shortened or lengthened according to the specific requirements and shape of the animal experimental table 18 to meet different experimental requirements.

[0068] The present invention has been described above by way of example with reference to the accompanying drawings. Obviously, the specific implementation of the present invention is not limited to the above-described manner. Any non-substantial improvements made using the inventive concept and technical solution of the present invention, or the direct application of the inventive concept and technical solution of the present invention to other occasions without modification, are all within the protection scope of the present invention.

Claims

1. A live observation bath for animal mesenteric microcirculation, comprising a water bath body (1) with an open upper part, characterized in that: A microcirculation observation ring (2) is set in the water bath tank (1). The microcirculation observation ring (2) is installed in the water bath tank (1) by an observation ring support frame (4). The upper surface of the microcirculation observation ring (2) is an upwardly convex arc surface.

2. The in vivo observation bath for animal mesenteric microcirculation according to claim 1, characterized in that: The microcirculation observation ring (2) is made of transparent material, and an illumination source is set inside the microcirculation observation ring (2).

3. The in vivo observation bath for animal mesenteric microcirculation according to claim 2, characterized in that: The lighting source is an LED light source (3).

4. The in vivo observation bath for animal mesenteric microcirculation according to claim 1, characterized in that: The water bath tank (1) is made of a material with thermosensitive constant temperature control performance.

5. The in vivo observation bath for animal mesenteric microcirculation according to claim 1, characterized in that: The observation ring support frame (4) is provided with an observation ring lifting mechanism (5).

6. The in vivo observation bath for animal mesenteric microcirculation according to claim 5, characterized in that: The observation ring lifting mechanism (5) adopts a screw drive mechanism. The screw drive mechanism has a nut on the microcirculation observation ring (2) and a vertically upward screw on the bottom surface of the water bath tank (1). The nut and the screw are threaded together. A horizontally arranged lifting adjustment transmission rod (6) is set on the water bath tank (1). A driven bevel gear is set on the screw. An active bevel gear is set at the inner end of the lifting adjustment transmission rod (6). The active bevel gear meshes with the driven bevel gear. The lifting adjustment transmission rod (6) extends out of the end of the water bath tank (1) and is equipped with a lifting adjustment knob (7).

7. The in vivo observation bath for animal mesenteric microcirculation according to claim 1, characterized in that: The observation bath is equipped with a camera (8); the camera (8) is mounted on a horizontal connecting rod (11), the horizontal connecting rod (11) is mounted on a camera support column (10), and a column base (12) is provided at the bottom of the camera support column (10).

8. The in vivo observation bath for animal mesenteric microcirculation according to claim 7, characterized in that: A camera illumination lamp (9) is provided on the side of the camera (8).

9. The in vivo observation bath for animal mesenteric microcirculation according to claim 7, characterized in that: The observation bath is equipped with an observation device control host (15); the observation device control host (15) is connected to the camera (8) via a signal line (13).

10. The in vivo observation bath for animal mesenteric microcirculation according to claim 1, characterized in that: The observation bath is equipped with a display (14), which is an interactive device for human-computer dialogue.

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