A sequencing library preparation cartridge, assembly method, and preparation instrument thereof

Abstract

The application provides a sequencing library preparation cartridge, an assembly method and a preparation instrument, relates to the technical field of gene sequencing, and comprises a shell, a reagent box, a moving assembly, and an interface. The top of the shell is provided with an opening. The reagent box is mounted in the shell and is in sealed connection with the shell. The reagent box has a plurality of storage cavities. The moving assembly is provided with a gun head and the interface. The interface is used to be coupled with an external driving mechanism. The moving assembly is rotationally connected in the shell and can move along the axial direction of the shell so that the gun head is selectively aligned with and inserted into the storage cavities. A sealed cavity is formed between the moving assembly and the reagent box. An assembly method of the cartridge comprises the following steps: S1, adding required reagents into the storage cavities in advance and sealing the storage cavities; S2, inserting the moving assembly into the opening at the bottom of the shell until the clamping groove is clamped on the protrusion and the through hole is sealed; and S3, inserting the reagent box into the opening at the bottom of the shell and connecting the reagent box on the bottom of the shell. The cartridge can be sealed to realize closed sequencing.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of gene sequencing technology, and in particular to a sequencing library preparation cartridge, an assembly method and a preparation instrument thereof. BACKGROUND

[0002] Since the 1950s, with the development of molecular genetics, people gradually realized that the essence of genes is a DNA fragment that controls biological traits and has genetic effects. Genes can faithfully replicate themselves to ensure the basic characteristics of organisms, and also can mutate to cause diseases. Gene sequencing can quickly and accurately obtain biological genetic information, reveal the complexity and diversity of genomes, and play a very important role in life science research; similarly, gene sequencing also has great significance and value for clinical medicine, and the mechanism research and clinical diagnosis of many diseases gradually rely on gene sequencing technology.

[0003] At present, the main way of traditional gene sequencing library preparation is still completed by manual operation. The processes of adding, warming reaction, purification and elution all need human participation to complete, and the whole process is completed in a relatively open laboratory environment. The disadvantage of such operation is that the environment is easy to contaminate the sample liquid. SUMMARY

[0004] In view of the deficiencies in the prior art, the first object of the present application is to provide a sequencing library preparation cartridge, which solves the problem that the cartridge in the prior art cannot achieve closed sequencing simply.

[0005] According to an embodiment of the present application, a sequencing library preparation cartridge comprises:

[0006] a housing, a top of which is provided with an opening for the driving mechanism to pass through,

[0007] a reagent box mounted in the housing and in sealed connection with the housing, the reagent box having a plurality of storage cavities;

[0008] a moving assembly configured with a gun head and an interface, the interface being used to couple with an external driving mechanism, the moving assembly being rotatably connected in the housing and being movable along the axial direction of the housing so as to selectively align and insert the gun head into the storage cavities, and a sealed cavity being formed between the moving assembly and the reagent box.

[0009] Compared with the prior art, the present application has the following beneficial effects:

[0010] 1. The kit is closed connected with the shell, and a closed cavity is formed between the moving assembly and the kit, so that the shell, the kit and the moving assembly form an integral closed space during operation; the driving mechanism outside is inserted into the opening and connected with the interface during use, and the driving mechanism can drive the moving assembly to rotate relative to the shell as needed, so that the gun head is rotated to align with different storage cavities, and the driving mechanism can also drive the moving assembly to move up and down along the axis of the shell, so that the gun head is pulled out or inserted into the storage cavity, and the gun head is connected with the liquid transfer mechanism to suck or discharge sample liquid, so that the sample liquid is moved in different storage cavities, and the whole sequencing process is carried out in the closed cartridge, which greatly reduces the influence of the environment on the sample liquid.

[0011] 2. In one aspect, only the moving assembly moves up and down or rotates circumferentially during the operation of the cartridge, which is beneficial for the arrangement and installation of the driving mechanism, and since the kit does not need to move, the kit itself and the positioning and installation with the preparation instrument are simple and convenient; on the other hand, the cartridge of the present application does not need to integrate the driving mechanism as consumables, which greatly simplifies the structure of the cartridge and reduces the cost of consumables.

[0012] Further, the shell is cylindrical, the outer wall of the kit is fitted with the inner wall of the shell, and the kit has a downwardly recessed limiting groove;

[0013] The moving assembly comprises:

[0014] a rod portion, one end of which is inserted into the limiting groove and can rotate relative to the limiting groove;

[0015] and a disc, which is fixed on the rod portion, and the outer wall of the disc is fitted with the inner wall of the shell.

[0016] Further, the ring side of the disc is bent downward and fitted on the inner wall of the shell.

[0017] Further, the rod portion is hollow, the interface is in the form of a notch, and it is recessed on the inner wall of the rod portion.

[0018] Further, the moving assembly is provided with through holes corresponding to the storage cavities one by one, and the through holes and the storage cavities are sealed by a hot melt film.

[0019] Further, the shell and the moving assembly are provided with a positioning module for positioning the moving assembly.

[0020] Further, the top of the shell is bent along the center of the shell, and the positioning module comprises a plurality of protrusions distributed on the bottom of the bent edge, and the moving assembly is fixed with a clamping groove which can be vertically clamped to the protrusions, and the bottom of the shell is provided with an opening.

[0021] Further, the connecting assembly and the kit are provided with an elastic assembly, which is used to drive the connecting assembly to move upward.

[0022] In view of the deficiencies in the prior art, a second object of the present application is to provide an assembling method of a cartridge, which solves the problem that the cartridge cannot be used for closed sequencing in the prior art.

[0023] An assembling method of a cartridge is used for the sequencing library preparation cartridge described above, and comprises the following steps:

[0024] S1, adding the required reagent into the storage cavity in advance and sealing;

[0025] S2, inserting the moving assembly into the opening at the bottom of the shell and moving upward until the clamping groove is clamped on the protrusion, and the through hole is sealed.

[0026] S3, inserting the reagent kit into the opening at the bottom of the shell and connecting it to the bottom of the shell.

[0027] Compared with the prior art, the present application has the following beneficial effects:

[0028] The moving assembly is assembled first, the moving assembly can be rotated according to the requirement, the clamping groove on the moving assembly is more smoothly aligned with the protrusion, the circumferential limiting of the moving assembly is realized, then the reagent kit is assembled at the bottom of the shell, the reagent kit is connected with the shell as a whole, the axial limiting of the limiting assembly is realized, the structure of the cartridge is simple and convenient to assemble, and the positioning is firm. In addition, after assembly, the staff can add another sample liquid through the through hole and finally into the storage cavity according to the requirement, the moving assembly is located at the top of the shell, the through hole on the moving assembly is sealed, and the closed cavity is formed between the moving assembly and the reagent kit.

[0029] In view of the deficiencies in the prior art, a third object of the present application is to provide a sequencing library preparation instrument, which solves the problem that the preparation instrument in the prior art cannot be used for closed sequencing.

[0030] A sequencing library preparation instrument is used for the sequencing library preparation cartridge described above, and comprises:

[0031] A rack is configured with a lifting mechanism and a rotating mechanism, the lifting mechanism can drive the rotating mechanism to move up and down, the rotating mechanism is configured with a connecting assembly, the free end of the connecting assembly is coupled with the interface to drive the rotating of the moving assembly, and the connecting assembly is provided with a pushing part which can abut on the end face of the moving assembly;

[0032] A pipetting mechanism is vertically movably connected to the rack through the lifting mechanism, and the pipetting mechanism is connected with the gun head to aspirate or extrude sample liquid.

[0033] Compared with the prior art, the present application has the following beneficial effects:

[0034] In use, the lifting mechanism drives the rotating mechanism to move downward until the free end of the connecting assembly passes through the opening of the shell and is connected with the interface, at this time the lifting mechanism continues to drive the rotating mechanism to move downward together with the connecting assembly to insert the gun head into the required storage cavity, and the pipetting mechanism aspirates sample liquid or discharges sample liquid into the storage cavity; the lifting mechanism drives the moving assembly to move upward or downward, and the rotating mechanism drives the moving assembly to rotate circumferentially, so as to realize the movement of sample liquid in different storage cavities, and the entire sequencing process is in the closed cartridge, which greatly reduces the influence of the environment on the sample liquid.

[0035] The rotating mechanism and the lifting mechanism are integrated into the preparation instrument, which reduces the use cost of the preparation cartridge as consumables; only the rotating mechanism and the lifting mechanism are needed to drive the moving assembly to move up and down and rotate as required during use, and the structure of the preparation cartridge and the preparation instrument is more compact, simple and convenient to connect. BRIEF DESCRIPTION OF DRAWINGS

[0036] Figure 1 It is a schematic diagram of the overall structure of the cartridge in the embodiment of the present application;

[0037] Figure 2 It is a schematic diagram of the internal structure of the cartridge; Figure 1

[0038] Figure 3 It is an exploded structural schematic diagram of the cartridge; Figure 1

[0039] Figure 4 It is an assembly schematic diagram of the cartridge and the connecting assembly in the embodiment of the present application;

[0040] Figure 5 It is a schematic diagram of the overall structure of the cartridge, the rotating mechanism, the lifting mechanism and the pipetting mechanism in the embodiment of the present application;

[0041] Figure 6 It is a structural schematic diagram of the preparation instrument in the embodiment of the present application.

[0042] In the above drawings:

[0043] 1, shell; 2, reagent box; 3, moving assembly; 4, lifting mechanism; 5, rotating mechanism; 6, pipetting mechanism; 7, pressing mechanism; 8, magnetic attraction mechanism; 9, cooling mechanism;

[0044] 101, protrusion;

[0045] 201, storage cavity; 202, clamping protrusion;

[0046] ​​301, gun head; 302, interface; 303, rod part; 304, disc; 305, through hole; 306, clamping groove;

[0047] 401, lifting plate;

[0048] 501, connecting assembly; 502, pipetting push-pull plate; 5011, liquid suction channel; 5012, upper disc; 5013, lower disc; 5014, round rod 5014; 5015, positioning column;

[0049] 601, piston rod; 602, connecting head;

[0050] 701, pressing plate. DETAILED DESCRIPTION

[0051] In order to make the purpose, technical scheme and beneficial effects of the present application clearer, the technical scheme of the present application will be further described below in combination with the drawings and examples. It should be understood that the specific examples described herein are only used to explain the present application and do not limit the present application.

[0052] Referring to Figures 1 to 3 The present application provides a sequencing library preparation cartridge, comprising:

[0053] A shell 1 is provided with an opening for the driving mechanism to pass through;

[0054] A reagent box 2 is mounted in the shell 1 and is in airtight connection with the shell 1. In order to facilitate the assembly and disassembly of the reagent box 2, the reagent box 2 is detachably connected to the shell 1. Specifically, the detachable connection can be a buckle connection, a bolt connection, a pin connection or the like. In the present embodiment, a clamping protrusion 202 is fixedly arranged on the side of the reagent box 2, and the clamping protrusion 202 is clamped in a clamping hole of the shell 1. The reagent box 2 has a plurality of storage cavities 201 for accommodating sample liquid and / or reagents. The storage cavities 201 can be shell-shaped cavities with an open top and a conical bottom. The storage cavities 201 are distributed around the axis of the reagent box 2. The specific number and size of the storage cavities 201 are determined according to the practical use requirements, and are not limited herein.

[0055] A moving assembly 3 is provided with a gun head 301 and an interface 302. The gun head 301 is preferably a TIP head. The top of the gun head 301 is open and is mounted on the moving assembly 3. The interface 302 is used to connect with the driving mechanism outside. The moving assembly 3 is rotatably connected in the shell 1 and can move along the axis of the shell 1, so that the gun head 301 is selectively aligned with and inserted into the storage cavities 201. An airtight cavity is formed between the moving assembly 3 and the reagent box 2.

[0056] In the present embodiment,

[0057] 1, the reagent box 2 is in close connection with the shell 1, and a closed cavity is formed between the moving assembly 3 and the reagent box 2, so that the shell 1, the reagent box 2 and the moving assembly 3 form an integral closed space during work (during the card box sequencing process, the gun head 301 is sealed through the connecting head 602, and each through hole 305 is sealed by hot melting of a film, which will be described in detail below); when in use, a driving mechanism outside is inserted into an opening and connected with the interface 302, so that the driving mechanism can drive the moving assembly 3 to rotate relative to the shell 1 as required, so that the gun head 301 is rotated to be aligned with different storage cavities 201, and the driving mechanism can also drive the moving assembly 3 to move up and down along the axis of the shell 1, so that the gun head 301 is pulled out of or inserted into the storage cavity 201, the gun head 301 is connected with the liquid transfer mechanism 6, sample liquid is sucked or discharged, and thus the sample liquid is moved in different storage cavities 201, and the whole sequencing process is carried out in the closed card box, so that the influence of the environment on the sample liquid is greatly reduced.

[0058] 2, on the one hand, only the moving assembly 3 moves up and down or rotates circumferentially during the working process of the card box, which is beneficial to the arrangement and installation of the driving mechanism, and since the reagent box 2 does not need to move, the structure of the reagent box 2 itself and the positioning and installation with the preparation instrument are simple and convenient; on the other hand, the card box of the present application does not need to integrate a driving mechanism as consumables, which greatly simplifies the structure of the card box and reduces the cost of consumables.

[0059] In another embodiment of the present application, the main difference from the above-mentioned embodiment is that the structure of the moving assembly 3 is optimized, as shown in Figure 2 and Figure 3 In view of the fact that in practice, additional reagents or sample liquid need to be added to the closed storage cavity 201 (for example, the reagent added later needs to be refrigerated or stored in a special environment, and it is not suitable to be added to the storage cavity 201 in advance), and after the addition is completed, the moving assembly 3 and the reagent box 2 need to form a closed cavity, a through hole 305 corresponding to each storage cavity 201 is arranged on the moving assembly 3, and the through hole 305 needs to be sealed after the reagent / sample liquid is added to the storage cavity 201; the sealing only needs to block the opening of the through hole 305, and specifically, the sealing mode includes but is not limited to covering a film at the opening of the through hole 305, and hot melting the film by using existing sealing equipment to achieve sealing, and the opening of the storage cavity 201 can also be sealed in the above-mentioned manner. In order to facilitate sealing of the through hole 305, the top end face of the moving assembly 3 is moved to the top end of the shell 1 during sealing; in this way, the applicability of the card box is enhanced and the sealing is convenient.

[0060] Further, the opening of the storage cavity 201 and the top of the through hole 305 are both arranged to be upwardly protruding, which is more conducive to sealing of the opening by the film.

[0061] Further, each through hole 305 is numbered, so that the operator can quickly identify the pre-added sample liquid in the storage cavity 201 corresponding to the through hole 305, and the operation efficiency and accuracy are improved.

[0062] Further, the storage cavity 201 is divided into at least a normal-temperature sequencing cavity and a refrigerated sequencing cavity, the normal-temperature sequencing cavity is used for loading normal-temperature sequencing sample liquid, and the refrigerated sequencing cavity is used for loading sequencing sample liquid that needs to be refrigerated.

[0063] In another embodiment of the present application, as shown in Figure 1 In order to simply and conveniently realize the sealed connection and assembly of the reagent box 2, the moving assembly 3 and the shell 1, the shell 1 is provided in a cylindrical shape, and the reagent box 2 is provided in a cylindrical shape, and specifically,

[0064] The outer wall of the reagent box 2 is attached to the inner wall of the shell 1, and the center of the reagent box 2 has a limiting groove recessed downward, and the inner wall of the limiting groove can be provided in a cylindrical surface, so that on the one hand, the rod part 303 (described below) can rotate in the limiting groove, and on the other hand, the rod part 303 can be limited and guided;

[0065] The moving assembly 3 comprises:

[0066] The rod part 303 is inserted into the limiting groove at one end and can rotate relative to the limiting groove, and the relative rotating mode of the rod part 303 and the limiting groove includes but is not limited to gap fit or bearing connection;

[0067] and the disc 304 is fixed on the rod part 303, and the outer wall of the disc 304 is attached to the inner wall of the shell 1; as an optimization, the disc 304 and the rod part 303 are integrally formed in this embodiment.

[0068] In this way, the outer wall of the reagent box 2 and the inner wall of the shell 1, and the outer wall of the disc 304 and the inner wall of the shell 1 can be simply and conveniently connected in a sealed manner, and of course, in order to improve the sealing effect, a sealing gasket or sealing oil can also be provided between them in practice; the disc 304, the rod part 303 and the limiting groove can together limit the left and right deflection of the moving assembly 3 and play a guiding role, and the structure of the reagent box is simple and the moving assembly 3 runs stably.

[0069] In another embodiment of the present application, as shown in Figure 1 and Figure 2As shown, the main difference from the above embodiment is that the structure of the disc 304 is improved, in order to improve the movement stability and sealing effect of the moving assembly 3, the contact surface between the outer wall of the disc 304 and the inner wall of the shell 1 can be increased, specifically, the ring side of the disc 304 is bent downward and fits on the inner wall of the shell 1, in this way, the disc 304 is less likely to shake left and right and is sealed well with the inner wall of the shell 1.

[0070] In another embodiment of the present application, as shown in Figure 1 and Figure 2 In order to consider the convenience when using the card box, on the basis of the above embodiment, a positioning module is arranged between the shell 1 and the moving assembly 3 to position the moving assembly 3, which plays a role in accurately positioning the initial relative position of the moving assembly 3 and the shell 1, so that when the card box is installed on the preparation instrument, the vertical movement of the connecting assembly 501 can be inserted into the interface 302, and the quick assembly of the connecting assembly 501 and the interface 302 is realized.

[0071] For example but not limited to, the top of the shell 1 is bent along the center to form a flange, the positioning module includes a plurality of protrusions 101 distributed on the bottom of the flange, the moving assembly 3 is fixedly provided with a clamping groove 306 that can be vertically clamped to the protrusions 101, and the bottom of the shell 1 is provided with an opening; in this way, the moving assembly 3 is inserted from the bottom of the shell 1, and the moving assembly 3 is adjusted by rotating to make the clamping groove 306 clamped on the protrusions 101, that is, the assembly of the moving assembly 3 and the shell 1 is completed, and the position of the moving assembly 3 relative to the shell 1 is fixed.

[0072] In another embodiment of the present application, on the basis of the above embodiment, an elastic assembly (not shown in the figure) is arranged between the connecting assembly 501 and the reagent box 2, which drives the connecting assembly 501 to move upward by elastic force, so as to reset the moving assembly 3 upward when the external force is removed; in order to simplify the structure of the card box, specifically, the elastic assembly includes a spring, the spring is sleeved on the rod portion 303, and the two ends of the spring abut against the opposite ends of the moving assembly 3 and the reagent box 2 respectively.

[0073] The present application also provides an assembly method of a card box, which is used for the above sequencing library preparation card box, and comprises the following steps:

[0074] S1, the required reagent is added into the storage cavity 201 in advance and sealed, specifically, a hot melting device can be used to cooperate with hot melt adhesive to seal, which is convenient for the gun head 301 to directly pierce the film on the top of the storage cavity 201 when moving downward;

[0075] S2, the moving assembly 3 is inserted from the opening at the bottom of the shell 1 and moves upward until the clamping groove 306 is clamped on the protrusion 101, and the sealing through hole 305 is sealed at this time;

[0076] S3, the kit 2 is inserted from the opening at the bottom of the shell 1 and connected to the bottom of the shell 1.

[0077] In this embodiment,

[0078] First, the moving assembly 3 is assembled, which can be rotated according to the needs, and at this time the elastic assembly has not been assembled, which can make the clamping groove 306 on the moving assembly 3 more smoothly rotate to align the protrusion 101, thereby achieving the circumferential limiting of the moving assembly 3, then the elastic assembly is sleeved on the rod part 303, finally the kit 2 is assembled at the bottom of the shell 1, the kit 2 is connected with the shell 1 as a whole, thereby achieving the axial limiting of the limiting assembly; the structure of the kit is simple and convenient to assemble, and the positioning is firm. In addition, after assembly, the staff can add another sample liquid through the through hole 305 and finally add it into the storage cavity 201 according to needs, and finally the moving assembly 3 is located at the top of the shell 1, which is convenient for sealing the through hole 305 on the moving assembly 3, thereby realizing the closed cavity between the moving assembly 3 and the kit 2.

[0079] The application further provides a sequencing library preparation instrument for the sequencing library preparation kit, as shown in the specification, Figures 4 to 6 as shown, comprising:

[0080] The rack should be understood as a platform built according to the position connection relationship of various parts in this embodiment, which is erected as needed in practice, and will not be described here;

[0081] In order to drive the moving assembly 3 to rotate, a rotating mechanism 5 is configured on the rack, which can be realized by a reduction motor, for example but not limited to, the rotating mechanism 5 is provided with a connecting assembly 501, both ends of the connecting assembly 501 are connected with the output end of the rotating mechanism 5 and the moving assembly 3 respectively, so as to transmit power to the moving assembly 3, in addition, a pushing part which can abut on the end face of the moving assembly 3 is arranged on the connecting assembly 501, which is used to push the moving assembly 3 to move downward; specifically, the connecting assembly 501 has an upper disc 5012 and a lower disc 5013 and a circular rod 5014 connecting the two, wherein the upper disc 5012 is detachably fixedly connected with the output end of the rotating mechanism 5 through bolts or screws and the like, the bottom end of the circular rod 5014 is provided with a thin shell-shaped positioning column 5015, the positioning column 5015 is coaxial with the circular rod 5014, and the inner diameter is smaller than that of the circular rod 5014, thereby forming a shaft shoulder, which plays the role of the above-mentioned pushing part; in this way, the structure of the connecting assembly 501 is simple, the positioning is reliable, and the connecting assembly 501 can be conveniently connected with and separated from the moving assembly 3;

[0082] In order to drive the rotating mechanism 5 to move up and down, thereby driving the moving assembly 3 to move together, a lifting mechanism 4 is further configured on the rack, for example but not limited to, the lifting mechanism 4 has a first linear driving assembly and a lifting plate 401;

[0083] Specifically, the lifting plate 401 is slidably connected to the rack through a vertical slide rail, the moving end of the first linear driving assembly is fixedly / detachably connected to the lifting plate 401, and the first linear driving assembly can be specifically selected from existing lead screws, electric push rods / cylinders, etc. The rotary mechanism 5 is installed on the lifting plate 401, and the first linear driving assembly can drive the rotary mechanism 5 to move up and down by driving the lifting plate 401;

[0084] In order to facilitate positioning and installation of the cartridge, the rack is further provided with an installation site, for example but not limited to, the installation site has a plurality of top-open positioning holes, the inner diameter of the positioning hole is slightly larger than the outer diameter of the storage cavity 201, and each positioning hole can be aligned with each storage cavity 201 one by one to ensure that the storage cavity 201 is smoothly inserted into the positioning hole to achieve positioning. In addition, a strip-shaped protruding rib can be provided at the bottom of the reagent box 2, and a bayonet matching the strip-shaped protruding rib is provided on the installation site. The strip-shaped protruding rib can also be quickly positioned by being clamped in the bayonet. The above two positioning and installation modes of the cartridge and the rack can be used alternatively or in combination.

[0085] In order to meet the addition, suction and transfer of the sample liquid in the reagent box 2, a pipetting mechanism 6 is provided, which is vertically movably connected to the rack through the lifting mechanism 4 to maintain the relative position of the pipetting mechanism 6 and the rotary mechanism 5. Specifically, in order to simplify the structure, the pipetting mechanism 6 is also installed on the lifting plate 401 in the embodiment, and a set of lifting mechanisms 4 can make the pipetting mechanism 6 and the rotary mechanism 5 rise or fall synchronously.

[0086] The pipetting mechanism 6 is connected to the gun head 301 to suck or extrude the sample liquid. Specifically, in order to improve the aesthetic appearance and play a protective role, the liquid suction channel 5011 is arranged in the interior of the connecting assembly 501. The liquid suction channel first vertically passes through the upper disc 5012 and the circular rod 5014, and then extends along the radial direction of the lower disc 5013.

[0087] The pipetting mechanism 6 has a piston rod 601, a second linear driving assembly, a connecting head 602 and a pipetting push-pull plate 502, wherein the piston rod 601 is movably connected at the top end of the liquid suction channel 5011 and can move along the vertical section of the liquid suction channel 5011, the top end of the connecting head 602 is installed at the bottom end of the liquid suction channel 5011, the other end of the connecting head 602 can be inserted into the interface 302 and the two are communicated, and it is to be noted that the middle part of the connecting head 602 is through, and the upper and lower ends of the connecting head 602 need to be sealed with the connecting assembly 501 and the moving assembly 3 respectively, so that the liquid can only pass through the middle part of the connecting head 602; specifically, the connecting head 602 can be a rubber head, and the sealing of the connecting position between the top end of the connecting head 602 and the connecting assembly 501 and the sealing of the connecting position between the bottom end of the connecting head 602 and the moving assembly 3 are realized by flexible deformation, of course, in practice, sealing glue can also be applied at the above-mentioned connecting positions, or a sealing ring can be additionally arranged to further enhance the sealing effect.

[0088] The pipetting push-pull plate 502 is vertically and slidingly connected to the lifting plate 401 through a slide rail, and the piston rod 601 and the second linear driving assembly are both connected to the pipetting push-pull plate 502, so that the second linear driving assembly can drive the piston rod 601 to move upward or downward as required, so as to suck or squeeze out the sample liquid, and the second linear driving assembly can also be a lead screw, an oil cylinder or an electric push rod.

[0089] In the embodiment,

[0090] 1. In use, the first linear driving assembly drives the lifting plate 401 to move downward, and then the rotating mechanism 5 moves downward until the free end of the connecting assembly 501 passes through the opening of the shell 1 and is connected with the interface 302, at this time, the bottom end of the connecting head 602 is clamped with the gun head 301, the lifting mechanism 4 continues to drive the rotating mechanism 5 to move downward together with the connecting assembly 501, so as to pierce the film with the gun head 301 and insert it into the required storage cavity 201, and then the pipetting mechanism 6 sucks the sample liquid or discharges the sample liquid into the storage cavity 201; the moving assembly 3 is driven by the lifting mechanism 4 to move upward or downward, and the moving assembly 3 is driven by the rotating mechanism 5 to rotate in a circumferential direction, so as to realize the movement of the sample liquid in different storage cavities 201, and the whole sequencing process is in a closed cartridge, which greatly reduces the influence of the environment on the sample liquid.

[0091] 2. The rotating mechanism 5 and the lifting mechanism 4 are integrated into the preparation instrument, which reduces the use cost of the preparation cartridge as consumables; during use, only the rotating mechanism 5 and the lifting mechanism 4 are needed to drive the moving assembly 3 to move up and down and rotate as required, and the structure of the preparation cartridge and the preparation instrument is more compact, simple and convenient to connect.

[0092] In another embodiment of the present application, Figure 5 and Figure 6As shown, on the basis of the above embodiment, the structure of the preparation instrument is optimized, in order to make the card box not move upward when the connecting assembly 501 moves upward, a pressing mechanism 7 is arranged on the rack, the pressing mechanism 7 is used to press the card box, specifically, the pressing mechanism 7 comprises a pressing plate 701 and a third linear driving assembly, wherein the pressing plate 701 is vertically slidably connected to the lifting plate 401 through a slide rail, the pressing plate 701 is provided with an avoiding slot for the connecting assembly 501 to pass through, the third linear driving assembly is connected with the pressing plate 701, and the third linear driving assembly can be an oil cylinder, an air cylinder or an electric push rod, etc., and the moving end thereof moves downward to drive the pressing plate 701 to move downward and press the top end of the shell 1.

[0093] Further, in order to adapt to the requirements of gene sequencing, the rack is further provided with a heating mechanism for heating the reagent box 2 and a cooling mechanism 9 for cooling the reagent box 2, and specifically, the heating mechanism and the cooling mechanism 9 can be existing Peltier heaters or coolers.

[0094] Further, in order to preserve the magnetic beads pre-stored in the storage cavity 201 when the liquid suction mechanism 6 suctions liquid, the rack is further provided with a magnetic attraction mechanism 8, the magnetic attraction mechanism 8 has magnets and is distributed outside the reagent box 2, and the magnetic attraction mechanism 8 can attract the magnetic beads to the wall surface of the reagent box 2 through magnetic force to avoid the magnetic beads from being sucked away by the liquid suction mechanism 6; specifically, the magnets of the magnetic attraction mechanism 8 are arranged on the side surface of the storage cavity 201, and the fourth linear driving assembly is used to drive the magnets to move towards the storage cavity 201, when the magnets are close to the magnetic beads, the magnetic force is increased to complete the work of attracting and retaining the magnetic beads; in addition, the magnets can also be used in the form of electromagnets to attract, and the size of the magnetic force can be changed through an on-off circuit, and the electromagnetic device itself is a prior art, which will not be described here.

[0095] In another embodiment of the present application, as shown in Figure 3 and Figure 4 The main difference between the above embodiment and the embodiment is that one kind of connection mode of the interface 302 is given, in order to simply and conveniently connect the interface 302 with the connecting assembly 501 (equivalent to an external driving mechanism), the interface 302 and the connecting assembly 501 can be clamped;

[0096] Specifically, the rod part 303 is hollow, the interface 302 is in the form of a slot with an open top, and the interface 302 is arranged on the inner wall of the rod part 303, and the outer wall of the positioning column 5015 is provided with a vertically extending clamping block; in this way, when the clamping block moves vertically downward to be inserted into the interface 302, the power of the rotating mechanism 5 can be transmitted to the moving assembly 3 through the connecting assembly 501, so that the moving assembly 3 rotates synchronously, the connecting assembly 501 moves downward, and when the shaft shoulder at the bottom of the connecting assembly 501 is in contact with the top end surface of the moving assembly 3, the moving assembly 3 can be pushed to move downward; in this way, the structures of the connecting assembly 501 and the moving assembly 3 are simple and light, the positioning column 5015 is inserted into the rod part 303, the guiding and limiting of the moving assembly 3 are strengthened, and the moving assembly 3 runs more stably; of course, if the above connection mode is adopted, the driving force for the upward movement of the moving assembly 3 comes from the elastic assembly; it should be noted that, because the clamping groove 306 of the moving assembly 3 is clamped with the protrusion 101 on the top of the shell 1 in the initial state, when the lifting mechanism 4 drives the connecting assembly 501 to move downward, the clamping groove 306 is already disengaged from the clamping with the protrusion 101, so that movement interference is avoided.

[0097] In another embodiment of the present application, the positioning column 5015 can be made of elastic material; in this way, when the positioning column 5015 is inserted into the rod part 303, the two are in interference fit, and the power can also be transmitted to the moving assembly 3 to drive the moving assembly 3 to rotate circumferentially or move up and down.

[0098] Those skilled in the art can understand that the above-described embodiments are exemplary, and those skilled in the art can make improvements, and the structures described in various embodiments can be freely combined without structural or principle conflicts. After the preferred embodiments of the present application are described in detail, those skilled in the art can clearly understand that various changes and modifications can be made without departing from the protection scope and spirit of the appended claims, and the present application is not limited to the implementation manners of the exemplary embodiments described in the specification.

Claims

1. A sequencing library preparation cartridge, comprising: It comprises: a shell (1) with an opening on the top for the driving mechanism to pass through, a kit (2) arranged in the shell (1) and connected hermetically, the kit (2) having multiple storage cavities (201); a moving assembly (3) arranged with a gun head (301) and an interface (302) for coupling with the external driving mechanism, the moving assembly (3) being rotatably connected in the shell (1) and axially movable along the shell (1) so that the gun head (301) is selectively aligned with and inserted into the storage cavities (201), a hermetic cavity being formed between the moving assembly (3) and the kit (2); the moving assembly (3) is provided with through holes (305) corresponding to the storage cavities (201) one by one, and the through holes (305) and the storage cavities (201) are both sealed by a hot melt film.

2. A sequencing library preparation cartridge of claim 1, wherein: The shell (1) is cylindrical, the outer wall of the kit (2) is fitted with the inner wall of the shell (1), and the kit (2) has a downwardly recessed limiting groove. The moving assembly (3) comprises: a rod portion (303) inserted into the limiting groove at one end and rotatable relative to the limiting groove; and a disc (304) fixed on the rod portion (303), the outer wall of the disc (304) being fitted with the inner wall of the shell (1).

3. A sequencing library preparation cartridge of claim 2, wherein, The ring side of the disc (304) is bent downward and fitted on the inner wall of the shell (1).

4. A sequencing library preparation cartridge of claim 3, wherein, The rod portion (303) is hollow, the interface (302) is notch-shaped, and it is recessed downward on the inner wall of the rod portion (303).

5. A sequencing library preparation cartridge according to any one of claims 1 to 4, wherein, The shell (1) and the moving assembly (3) are provided with a positioning module for positioning the moving assembly (3).

6. A sequencing library preparation cartridge of claim 5, wherein, The top of the shell (1) is bent along the center of the shell (1), the positioning module comprises a plurality of protrusions (101) distributed on the bottom of the bent edge, the moving assembly (3) is provided with a clamping groove (306) vertically clamped to the protrusions (101), and the bottom of the shell (1) is provided with an opening.

7. A method of assembling a cartridge, characterized by, A sequencing library preparation cartridge as claimed in claim 6, comprising the following steps: S1, adding the required reagents into the storage cavities (201) in advance and sealing them; S2, inserting the moving assembly (3) from the opening at the bottom of the shell (1) and moving it upward until the clamping groove (306) is clamped on the protrusions (101) and the through holes (305) are sealed; S3, inserting the kit (2) from the opening at the bottom of the shell (1) and connecting it to the bottom of the shell (1).

8. A sequencing library preparation instrument, comprising: A sequencing library preparation cartridge as claimed in any one of claims 1 to 4, comprising: a rack arranged with a lifting mechanism (4) and a rotating mechanism (5), the lifting mechanism (4) being capable of driving the rotating mechanism (5) to move up and down, the rotating mechanism (5) being arranged with a connecting assembly (501), the free end of the connecting assembly (501) being coupled with the interface (302) to drive the moving assembly (3) to rotate, the connecting assembly (501) being provided with a pushing portion capable of abutting against the end face of the moving assembly (3). A pipetting mechanism (6) is vertically movably connected to the frame by the lifting mechanism (4), and the pipetting mechanism (6) is communicated with the gun head (301) to aspirate or extrude sample liquid.

9. A sequencing library preparation instrument as claimed in claim 8, wherein, An elastic assembly is arranged between the connecting assembly (501) and the kit (2), and the elastic assembly is used to drive the connecting assembly (501) to move upward.

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