A biological pesticide for preventing and controlling wild rice rust

Abstract

The invention belongs to the technical field of pesticide research and development, and specifically relates to a biological pesticide for preventing and treating wild rice rust. A biological pesticide for preventing and treating wild rice rust, wherein the effective component is formed by a binary compound of carvacrol and diniconazole or carbendazim. The binary compound of carvacrol and diniconazole or carbendazim in the biological pesticide of the invention has a synergistic effect on wild rice rust, can improve the prevention and treatment effect of wild rice rust, help reduce the dosage of pesticide application, reduce the prevention and treatment cost, and reduce the pesticide residue in wild rice.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticide research and development, and particularly relates to a biological pesticide for preventing and treating wild rice rust. Background Art

[0002] Zizania latifolia is the second largest aquatic vegetable in my country and is also a unique vegetable in my country. Zizania latifolia rust is caused by infection with the Basidiomycetes fungus Uromyces coronatus and is the main disease in Zizania latifolia production. Zizania latifolia rust mainly harms Zizania latifolia leaves. In severe cases, it may even cause the entire leaf to turn yellow, and the resulting Zizania latifolia will be very thin, which has a great impact on Zizania latifolia yield. Data show that the incidence of Zizania latifolia leaves can reach more than 70%, and the yield loss can reach 15-30%.

[0003] Continuous planting of a single wild rice variety for many years has caused frequent outbreaks of wild rice rust. In the absence of excellent disease-resistant varieties, chemical control is still the main way to control the epidemic of the disease. Commonly used chemical agents include 25% pyraclostrobin water dispersible granules, 10% difenoconazole water dispersible granules and 12.5% ​​diniconazole wettable powder. The application of chemical agents is one of the most effective means of controlling plant diseases and insect pests. However, the use of chemical agents to control diseases and insect pests is likely to cause resistance to the drugs. At the same time, the long-term continuous high-dose application of a single chemical agent is also likely to cause a series of problems such as drug residues and environmental pollution. This also makes the control effect of existing chemical agents on wild rice rust decrease year by year. Therefore, it is necessary to develop new agents to improve the control effect of wild rice rust and ensure the yield and quality of wild rice.

[0004] Compounding chemical agents with different components can be used to determine whether a certain combination is synergistic, additive, or antagonistic based on the actual application effect. Among them, the formula with good compounding synergy can significantly improve the actual prevention and control effect, reduce the application dosage of pesticides, and greatly delay the development of pesticide resistance in pests and diseases. It is one of the effective methods for developing new agents.

[0005] Biopesticides refer to preparations that use living organisms or their metabolism to kill or inhibit agricultural pests, including botanical pesticides, animal pesticides, and microbial pesticides. Carvacrol is a pure botanical fungicide extracted and processed from yellow flower buds. It has a strong antibacterial effect and can effectively inhibit the growth of pathogenic bacteria spores and hyphae. It is safe, environmentally friendly, and has no phytotoxicity. At the same time, it has good control effects on a variety of fungal diseases such as powdery mildew, rust, and downy mildew. Application No. CN201510084511.0 discloses a fungicide composition containing penflufenpyram and carvacrol and its use, and specifically discloses that penflufenpyram and carvacrol have good control effects on powdery mildew or rust occurring in wheat, fruit trees, flowers, and vegetables after compounding.

[0006] The inventors found through indoor biological activity tests that carvacrol, when combined with silthiopyrad, diniconazole or carbendazim, exhibits a synergistic effect in preventing and controlling Zizania latifolia rust.

[0007] At present, there are no reports on the combination of carvacrol with silthiopyrad, diniconazole or carbendazim. Summary of the invention

[0008] The object of the present invention is to provide a biological pesticide for preventing and controlling water bamboo shoot rust, which can improve the prevention and control effect of water bamboo shoot rust, reduce the application dosage of the pesticide, delay the generation of drug resistance of pathogens, and can be used to develop new agents.

[0009] To achieve the above object, the present invention provides the following technical solutions:

[0010] A biological pesticide for preventing and treating wild rice rust, wherein the active ingredient is a binary compound of carvacrol and diniconazole or carbendazim.

[0011] Preferably, the mass ratio of carvacrol to diniconazole is 1-9:20-1.

[0012] Preferably, the mass ratio of carvacrol to carbendazim is 1-40:15-1.

[0013] Another object of the present invention is to provide a pesticide preparation, which includes the biological pesticide for preventing and controlling wild rice rust, and the rest are auxiliary ingredients that can be added in pesticide science.

[0014] Compared with the prior art, the present invention has the following beneficial effects:

[0015] (1) The active ingredient eugenol in the biopesticide composition of the present invention has a synergistic effect on wild rice rust after being mixed with diniconazole or carbendazim, which can improve the control effect of wild rice rust, help reduce the dosage of pesticide application, reduce the control cost, and reduce the pesticide residue in wild rice.

[0016] (2) The active ingredient eugenol in the biopesticide composition of the present invention has a different bactericidal mechanism from diniconazole or carbendazim, and can effectively delay the development of drug resistance in pathogens, extend the service life of the agent, and can be used to develop new agents. DETAILED DESCRIPTION

[0017] The present invention can be better understood according to the following examples. However, it is easy for those skilled in the art to understand that the contents described in the examples are only used to illustrate the present invention, and should not and will not limit the present invention described in detail in the claims.

[0018] Example :Indoor bioactivity test of carvacrol complex against Zizania latifolia rust

[0019] 1. Test strain: Fresh summer spores of Zizania coronae, collected from infected Zizania coronae plants.

[0020] 2. Test reagents

[0021] 92.5% carvacrol technical (Shanghai Yuanye Biotechnology Co., Ltd.);

[0022] 98% silthiopyrad technical (Hebei Xingbai Agricultural Technology Co., Ltd.);

[0023] 95% diniconazole technical (Jiangsu Qizhou Green Chemical Co., Ltd.);

[0024] 98% Carbendazim technical (Jiangsu Huifeng Bio-Agriculture Co., Ltd.).

[0025] The above-mentioned test agents were first dissolved in dimethyl sulfoxide, and then diluted with 0.1% Tween-80 aqueous solution to prepare a single-dose stock solution. Multiple groups of proportions were set, and each single dose and proportioned mixture was set with 5 mass concentration gradients according to the equal ratio method.

[0026] 3. Test method (refer to "NY / T 1156.1-2006 Guidelines for Indoor Bioassay Tests of Pesticides - Fungicides Part 1: Test for Inhibition of Germination of Pathogenic Fungal Spores - Concave Slide Method").

[0027] Collect fresh summer spores from infected Zizania latifolia plants and resuspend the spores in deionized water to 1 × 10 5 to 1×10 7 spores and add 0.5% glucose solution.

[0028] Use a pipette to pipette 0.5mL of the drug solution from low concentration to high concentration and add them to the small test tubes respectively, then pipette 0.5mL of the prepared spore suspension to mix the drug solution and spore suspension in equal amounts. Use a micropipette to pipette the above mixed solution onto a concave glass slide, then place it in a culture dish with a shallow layer of water, cover it and place it in a 25℃ incubator. Set up 3 replicates for each treatment, and set the treatment of 0.1% Tween-80 aqueous solution as a blank control.

[0029] When the spore germination rate of the blank control reached more than 90%, the spore germination was checked and processed. Three fields of view were randomly observed in each treatment and repeated, and a total of 200 spores were investigated. The number of germination and the total number of spores were recorded respectively. The spore tube length greater than the short radius of the spore was considered to be germinated. Based on the survey data, the relative inhibition rate of spore germination of each treatment was calculated.

[0030]

[0031]

[0032]

[0033] 4. Data analysis: DPS software was used for data statistical analysis. The logarithmic value of fungicide concentration was taken as x and the corresponding relative inhibition rate of spore germination probability value was taken as y for linear regression. The toxicity regression equation and the toxicity EC value of the agent to the target pathogen were obtained. 50 The co-toxicity coefficient (CTC) was calculated according to the Sun Yunpei method.

[0034]

[0035] In the above formula: ATI--the toxicity index of the mixture; S--the EC of the standard agent 50 , the unit is mg / L; M--EC of the mixture 50 , unit is mg / L.

[0036] TTI=TI A ×P A +TI B ×P B

[0037] In the above formula: TTI-theoretical toxicity index of the mixture; TI A --Toxicity index of A agent; P A --The percentage of agent A in the mixture, in percentage (%); TI B --Toxicity index of agent B; P B --The percentage of agent B in the mixture, in percentage (%).

[0038]

[0039] In the above formula: CTC--co-toxicity coefficient; ATI--actual toxicity index of the mixture; TTI--theoretical toxicity index of the mixture.

[0040] 5. Measurement results

[0041] The synergistic effect of the drug was evaluated based on the calculated co-toxicity coefficient (CTC), CTC≤80 was antagonistic, 80<CTC<120 was additive, and CTC≥120 was synergistic. The results are shown in Table 1-3.

[0042] Table 1 Indoor bioactivity test of carvacrol and silthiopyrad against Zizania odorifera rust pathogen

[0043] Drug name and ratio <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC Carvacrol 24.5846 100.0000 -- -- Sithiopyrad 9.7613 251.8578 -- -- Carvacrol 1: silthiopyrad 7 7.1211 345.2360 232.8756 148.2491 Carvacrol 1: silthiopyrad 5 6.1408 400.3485 226.5482 176.7167 Carvacrol 1: silthiopyrad 3 8.1083 303.2029 213.8934 141.7542 Carvacrol 1: Silthiopyrad 1 10.0628 244.3117 175.9289 138.8696 Carvacrol 3: silthiopyrad 1 12.4932 196.7839 137.9645 142.6337 Carvacrol 5: Silthiopyrad 1 9.4200 260.9830 125.3096 208.2705

[0044] As shown in Table 1, after the active ingredients carvacrol and silthiopyrad were compounded, the co-toxicity coefficients against the pathogen of Zizania latifolia were all greater than 120 within the mass ratio of 1-5:7-1, showing a synergistic effect.

[0045] Table 2 Indoor bioactivity test of carvacrol and diniconazole complex against wild rice rust pathogen

[0046]

[0047]

[0048] As shown in Table 2, after the active ingredients carvacrol and diniconazole were compounded, the co-toxicity coefficients against the pathogen of Zizania latifolia rust were all greater than 120 within the mass ratio of 1-9:20-1, showing a synergistic effect.

[0049] Table 3 Indoor bioactivity test of carvacrol and carbendazim against wild rice rust pathogens

[0050] Drug name and ratio <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC Carvacrol 24.5846 100.0000 -- -- Carbendazim 23.1117 106.3730 -- -- Carvacrol 1: Carbendazim 15 17.0061 144.5634 105.9747 136.4132 Carvacrol 1: Carbendazim 10 16.2915 150.9045 105.7936 142.6404 Carvacrol 1: Carbendazim 5 12.5888 195.2895 105.3108 185.4411 Carvacrol 1: Carbendazim 3 14.1544 173.6887 104.7797 165.7656 Carvacrol 1: Carbendazim 1 11.0878 221.7266 103.1865 214.8795 Carvacrol 3: Carbendazim 1 18.1152 135.7126 101.5932 133.5842 Carvacrol 5: Carbendazim 1 20.0182 122.8112 101.0622 121.5205 Carvacrol 10: Carbendazim 1 16.2907 150.9119 100.5794 150.0426 Carvacrol 15: Carbendazim 1 17.1861 143.0493 100.3983 142.4818 Carvacrol 20: Carbendazim 1 13.6796 179.7172 100.3035 179.1735 Carvacrol 30: Carbendazim 1 10.7408 228.8898 100.2056 228.4203 Carvacrol 40: Carbendazim 1 12.1569 202.2275 100.1554 201.9137

[0051] As shown in Table 3, after the active ingredient carvacrol was compounded with carbendazim, the co-toxicity coefficient against the pathogen of Zizania latifolia was greater than 120 within the mass ratio of 1-40:15-1, showing a synergistic effect.

[0052] In summary, the combination of eugenol with silthiopyrad, diniconazole or carbendazim has a synergistic effect on water bamboo rust, which can improve the prevention and control effect of water bamboo rust, help reduce the dosage of pesticide application, reduce the cost of prevention and control, and reduce the pesticide residues in water bamboo.

[0053] The above is only a preferred embodiment of the present invention. Those skilled in the art may make appropriate improvements without departing from the principle of the present invention. These improvements are also within the protection scope of the present invention.

Claims

1. A biological pesticide for preventing and treating wild rice rust, characterized in that: The effective component is prepared by compounding carvacrol and diniconazole in binary form; the mass ratio of carvacrol to diniconazole is 1-9:20-1.

2. A pesticide preparation, characterized in that: It includes the biological pesticide for preventing and controlling wild rice rust as claimed in claim 1.

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