An application of RHPS4

Abstract

The present invention provides an application of RHPS4, applying RHPS4 in the aspect of anti-plant virus, the plant viruses including common plant viruses such as tobacco mosaic virus, potato virus Y, and cucumber mosaic virus, etc. The drug ingredient required for anti-plant virus is 3,11-difluoro-6,8,13-trimethyl-8H-quinoline[4,3,2-kl]acridinium methyl sulfate (RHPS4); RHPS4 effectively inhibits the proliferation of tobacco mosaic virus (TMV). The inactivation activity of 1.0 mmol / L RHPS4 against TMV reaches 74.30%, the protection activity reaches 62.27%, and the therapeutic activity reaches 51.61%. After treating tobacco with RHPS4, the growth state of the tobacco is good, without obvious inhibition or poor growth phenomenon, indicating that RHPS4 is safe for host plants and has no phytotoxic effect, demonstrating that RHPS4 has the potential to be developed into a plant virus inhibitor.

Description

Technical Field

[0001] The present invention relates to the field of agricultural drugs, and specifically to an application of RHPS4. Background Art

[0002] Plant virus diseases, known as "plant cancers", are diseases caused by plant viruses infecting plants. These diseases can occur in crops such as rice, vegetables, fruit trees, and tobacco, causing great losses to crop production. Plant virus diseases are the second largest category of plant diseases after plant fungal diseases. The entire life activities of plant viruses occur within plant cells, and the substances, energy, and sites required for virus replication are completely provided by plant cells. Moreover, plants do not have a complete immune system, so the prevention and control of plant virus diseases are extremely difficult. Plant virus diseases have a high incidence and spread rapidly, and have characteristics such as explosiveness, migration, and intermittence. As a global disease, plant virus diseases cause economic losses of up to $15 billion worldwide every year. In recent years, with the expansion of crop planting scale and the rise in temperature, the occurrence rate of plant virus diseases has increased exponentially. Plant virus diseases can reduce crop yields by 50% - 60%, or even lead to crop failure. And new plant viruses frequently appear. Therefore, effectively preventing and controlling plant virus diseases is of great significance for the development of China's national economy.

[0003] At present, chemical control is an important method for preventing and controlling plant viruses. However, there is a lack of control agents for plant virus diseases in China, and the control effect on new plant viruses is very limited. Therefore, there is an urgent need to develop a drug that can efficiently inhibit plant viruses. Summary of the Invention

[0004] Aiming at the deficiencies of the existing technology, the purpose of the present invention is to provide an application of RHPS4 to solve the problems raised in the above background art. The structure of the present invention is novel. After treating tobacco with RHPS4, the growth state of the tobacco is good, without obvious inhibition or poor growth phenomena, indicating that RHPS4 is safe for host plants and has no phytotoxic effect.

[0005] To achieve the above purpose, the present invention is realized through the following technical solutions: An application of RHPS4 in anti-plant virus.

[0006] Preferably, the plant viruses include common plant viruses such as tobacco mosaic virus, potato virus Y, and cucumber mosaic virus.

[0007] More preferably, the drug component required for anti-plant virus is 3,11-difluoro-6,8,13-trimethyl-8H-quinoline[4,3,2-kl]acridinium methyl sulfate (RHPS4).

[0008] Advantages of the present invention: An application of RHPS4 according to the present invention,

[0009] 1. In the application test of RHPS4, the antiviral activity of RHPS4 against plant viruses was evaluated by the half-leaf lesion method. The results showed that RHPS4 effectively inhibited the proliferation of TMV. The inactivation activity of 1.0 mmol / L RHPS4 against TMV reached 74.30%, the protective activity reached 62.27%, and the therapeutic activity reached 51.61%, and it showed a dose-dependent relationship.

[0010] 2. After the tobacco was treated with RHPS4 in the application of RHPS4, the growth state of the tobacco was good, and there was no obvious inhibition or poor growth phenomenon, indicating that RHPS4 is safe for host plants and has no phytotoxic effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0011] Figure 1 It is the molecular formula of the RHPS4 compound for the application of RHPS4 according to the present invention;

[0012] Figure 2 It is a schematic diagram of the inactivation activity of RHPS4 against TMV for the application of RHPS4 according to the present invention;

[0013] Figure 3 It is a schematic diagram of the protective activity of RHPS4 against TMV for the application of RHPS4 according to the present invention;

[0014] Figure 4 It is a schematic diagram of the therapeutic activity of RHPS4 against TMV for the application of RHPS4 according to the present invention;

[0015] Figure 5 It is a schematic diagram of the inhibition of TMV by RHPS4 for the application of RHPS4 according to the present invention;

[0016] Figure 6 It is a schematic diagram of the effect of 5.0 mmol / L RHPS4 on the growth of Xanthi tobacco for the application of RHPS4 according to the present invention. DETAILED DESCRIPTION OF THE INVENTION

[0017] In order to make the technical means, creative features, achieved purposes and effects of the present invention easy to understand, the present invention will be further described below in conjunction with specific embodiments.

[0018] Please refer to Figures 1 to 6 , the present invention provides a technical solution: an application of RHPS4, applying RHPS4 in the aspect of anti-plant virus.

[0019] The plant viruses include common plant viruses such as tobacco mosaic virus, potato virus Y, and cucumber mosaic virus.

[0020] The drug ingredient required for anti - plant virus is 3,11 - difluoro - 6,8,13 - trimethyl - 8H - quinoline[4,3,2 - kl]acridinium methyl sulfate (RHPS4).

[0021] The application test of the said RHPS4 in inhibiting plant viruses includes the following steps:

[0022] 1) Dissolve the RHPS4 compound in distilled water to concentrations of 0.2, 0.5, and 1.0 mmol / L;

[0023] 2) Take tobacco mosaic virus in plant viruses as the experimental object. Mix the RHPS4 compound solution with the virus extract at different concentrations and smear it on one side of the tobacco leaf, and smear the TMV extract without RHPS4 on the other side. Observe the growth of both sides of the leaf on the fourth day, and calculate the inactivation activity of the RHPS4 compound against TMV through the number of leaf lesions;

[0024] 3) Smear the RHPS4 compound solution at different concentrations on one side of a healthy tobacco leaf, and leave the other side untreated as a reference. On the second day, smear an equal amount of TMV extract on both sides of the tobacco leaf and continue to culture for four days. Observe the growth of both sides of the leaf and calculate the protective activity of the RHPS4 compound in inhibiting TMV through the number of leaf lesions;

[0025] 4) Smear the RHPS4 compound solution at different concentrations on one side of the leaf of a tobacco plant infected with TMV, and leave the other side untreated as a reference. Observe the growth of both sides of the leaf on the fourth day, and calculate the therapeutic activity of the RHPS4 compound in inhibiting TMV through the number of leaf lesions;

[0026] 5) Calculate the inhibition rate of the RHPS4 compound against TMV through the number of leaf lesions (the calculation formula is: inhibition rate (%) = [(C - T) / C]×100%, where "C" represents the average number of lesions on the control half - leaf, and "T" represents the average number of lesions on the half - leaf treated with RHPS4);

[0027] 6) Obtain the results: evaluate the inactivation activity of RHPS4 in inhibiting TMV, evaluate the protective activity of RHPS4 in inhibiting TMV, evaluate the therapeutic activity of RHPS4 in inhibiting TMV, and evaluate the toxicity of RHPS4 to tobacco;

[0028] As Figure 2 shown, the test steps for evaluating the inactivation activity of RHPS4 in inhibiting TMV:

[0029] 1. Incubate the virus: Add the TMV extract (final concentration of TMV is 1.0 μg / mL) to the RHPS4 aqueous solution, mix gently, incubate on ice for 30 min, and add the TMV extract at the same concentration to water as the control group.

[0030] 2. Rub inoculation: Use Nicotiana tabacum cv. Xanthi at the 6-7 leaf stage as the test tobacco. Add an appropriate amount of quartz sand to the leaves of the test Nicotiana tabacum cv. Xanthi. Use a gauze swab to apply 100 μL of the RHPS4 and virus mixture treatment group along the main vein of the leaf on the right half of the tobacco leaf, and apply 100 μL of the control group on the left half leaf. Count the number of local leaf lesions on the 4th day after inoculation.

[0031] As Figure 3 shown, the test steps for evaluating the protective activity of RHPS4 against TMV are as follows:

[0032] 1. Apply the drug: Use Nicotiana tabacum cv. Xanthi at the 6-7 leaf stage as the test tobacco. Take 100 μL of the RHPS4 aqueous solutions of 0.2, 0.5, and 1.0 mmol / L, and apply them along the main vein of the leaf on the right half of the tobacco leaf through a gauze swab. Continue to culture at 25 °C for 24 h, and apply distilled water to the left half leaf as the blank control group.

[0033] 2. Apply the virus: Add an appropriate amount of quartz sand to the leaves of the test Nicotiana tabacum cv. Xanthi. Take 100 μL of the TMV extract (final concentration of TMV is 1.0 μg / mL), and apply it to the surface of the Nicotiana tabacum cv. Xanthi leaf through a gauze swab. Count the number of local leaf lesions on the 4th day after inoculation.

[0034] As Figure 4 shown, the test steps for evaluating the therapeutic activity of RHPS4 against TMV are as follows:

[0035] 1. Apply the virus: Add an appropriate amount of quartz sand to the leaves of the test Nicotiana tabacum cv. Xanthi. Take 100 μL of the TMV extract (final concentration of TMV is 1.0 μg / mL), and apply it along the main vein of the leaf on the right half of the tobacco leaf through a gauze swab. Continue to culture at 25 °C for 24 h.

[0036] 2. Apply the drug: Take 100 μL of the RHPS4 compound, and apply it to the surface of the Nicotiana benthamiana leaf through a gauze swab. Continue to culture for 96 h, and count the number of local leaf lesions.

[0037] As Figure 6 shown, the test steps for evaluating the toxicity of RHPS4 to tobacco are as follows:

[0038] 1. Compound preparation: Dissolve RHPS4 in distilled water to a concentration of 5.0 mmol / L.

[0039] 2. Smearing compound: Use Xanthi tobacco at the 6-7 leaf stage as the test tobacco. Use a gauze swab to smear 100 μL of RHPS4 along the main vein of the leaf on the tobacco leaf. Observe the growth condition of the leaf on the fourth day (whether wilting, yellowing, wrinkling, etc. occur).

[0040] It can be known from the test results that the antiviral activity of RHPS4 against plant viruses was evaluated by the half-leaf lesion method. The results showed that RHPS4 effectively inhibited the proliferation of tobacco mosaic virus TMV. The inactivation inhibitory activity of 1.0 mmol / L RHPS4 against TMV reached 74.30%, the protective activity reached 62.27%, and the therapeutic activity reached 51.61%. After treating tobacco with RHPS4, the growth state of the tobacco was good, without obvious inhibition or growth problems, indicating that RHPS4 is safe for host plants and has no phytotoxic effect, suggesting that RHPS4 has the potential to be developed into a plant virus inhibitor.

[0041] The above shows and describes the basic principles, main features and advantages of the present invention. For those skilled in the art, it is obvious that the present invention is not limited to the details of the above exemplary embodiments, and can be implemented in other specific forms without departing from the spirit or basic features of the present invention. Therefore, from any point of view, the embodiments should be regarded as exemplary and non-limiting. The scope of the present invention is defined by the appended claims rather than the above description. Therefore, all changes falling within the meaning and scope of the equivalent elements of the claims are intended to be included in the present invention. Any reference signs in the claims should not be regarded as limiting the claimed rights.

[0042] In addition, it should be understood that although this specification is described according to embodiments, not every embodiment only contains an independent technical solution. This narrative way of the specification is only for clarity. Those skilled in the art should regard the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. An application of RHPS4, Characterized in that: RHPS4 is applied in the prevention and control of tobacco mosaic virus.

Images