A plant-extracted volatile oil with fatigue-relieving and analgesic effects, and its preparation and application

Abstract

The present invention discloses a plant-extracted volatile oil having fatigue-relieving and analgesic effects, and its preparation and application. The technical field of traditional Chinese medicine includes pepper oil, pepper oil, camphor bark oil, camphor leaf oil, eucalyptus oil, peppermint oil, peppermint oil, ginger oil, clove bud oil, clove leaf oil, cinnamon oil, cassia oil, fennel oil, star anise oil, clove basil oil, wormwood oil, thyme oil, linalool thyme oil, patchouli oil, nutmeg oil, notopterygium oil, angelica dahurica oil, spatholobi oil, eucommia oil, diatomaceous earth oil, sedge oil, saposhnikovia oil, Perilla leaf oil, magnolia flower oil, calamus oil, ginkgo leaf oil, tangerine peel oil, rose oil, rhodiola rosea oil, ginseng oil, atractylodes oil, atractylodes oil, magnolia oil, chuanxiong oil, safflower oil, saffron oil, angelica oil, angelica oil, salvia oil, frankincense oil, myrrh oil, benzoin oil, storax oil, costus root oil, cyperus oil, agarwood oil, sandalwood oil, dalbergia oil, menthol, ethanol, vitamin E oil and grape seed oil.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese medicine, and more particularly to a plant-extracted volatile oil having fatigue-relieving and analgesic effects, and the preparation and application thereof. Background Art

[0002] Traditional Chinese medicine has a long history, and the application of aromatic Chinese medicine and aromatherapy has a long history and rich clinical experience in China. With the promulgation of the first volume of the 2020 edition of the Chinese Pharmacopoeia, plant oils and extracts have become a separate chapter, and the corresponding implementation standards and inspection methods have been clarified. However, compared with the vigorous development of aromatherapy internationally, aromatherapy in my country is still stuck in the traditional medical decoction and extraction method or the application of a single effective substance, lacking the research and application of modern preparations.

[0003] Chronic Fatigue Syndrome (CFS) is a long-term sub-health state characterized by persistent or recurrent fatigue without a clear cause, accompanied by non-specific manifestations such as decreased memory or attention, headache and body pain, muscle pain (or weakness and discomfort, flu-like symptoms) and sleep disorders. Due to changes in modern lifestyles and the accelerated pace of life, the incidence of CFS has increased year by year. The incidence of CFS is about 0.2% to 2.54%, and it is more common in middle-aged women, with a male-female incidence ratio of about 1:2. Long-term chronic fatigue reduces the quality of life of patients, increases medical expenses, and reduces social productivity.

[0004] Modern medicine believes that its pathogenesis mainly includes: 1. Neuroendocrine disorders: Neuroendocrine disorders may be related to CFS, especially the dysfunction of the neurotransmitter 5-hydroxytryptamine (5-HT) system and the decreased activity of the hypothalamo-pituitary-adrenal axis (HPA) system. 2. Immune dysfunction: CFS may have immune dysfunction, including chronic immune activation, decreased responsiveness of immune cells to polyclonal stimulation, changes in inflammation and cytokines, and the production of self and other special antibodies. High levels of pro-inflammatory factors can lead to fatigue and flu-like symptoms. 3. Oxidative stress: There is evidence that CFS is accompanied by increased inflammatory activation, oxidative stress and nitrification stress. Traditional Chinese medicine attributes it to the categories of "deficiency" and "lily disease", and the pathogenesis is deficiency of qi, blood, yin and yang, and dysfunction of internal organs.

[0005] Conventional Western medicine treatment methods are nothing more than oral antidepressants or other symptomatic drugs, such as muscle relaxants and non-steroidal anti-inflammatory analgesics. It is now popular both at home and abroad to use plant essential oil aromatherapy (clary sage, lavender, etc.) combined with physical therapy and music to treat chronic fatigue syndrome; among them, Chinese medicine has achieved very good therapeutic effects by relying on external treatment techniques (massage, moxibustion, cupping, etc.) and various types of acupuncture (traditional acupuncture, ear acupuncture, press needle) that have been passed down for thousands of years. However, due to the long onset period and easy recurrence of chronic fatigue syndrome, patients need to go to medical institutions for treatment every week. The number of professional and technical personnel in traditional Chinese medicine external treatment in general community medical institutions is limited, and weekly treatment is likely to affect the patient's work, which in turn increases the patient's work pressure and stimulates the recurrence of chronic fatigue syndrome.

[0006] In summary, how to provide a natural plant-extracted volatile oil is a problem that those skilled in the art need to solve urgently. Summary of the invention

[0007] In view of this, the present invention provides a plant-extracted volatile oil with fatigue-relieving and analgesic effects, and the preparation and application thereof.

[0008] Its realization mainly relies on the analysis and organization of prescription models with rich clinical experience in Meta and CiteSpace related literature of traditional Chinese medicine, combined with the principle of promoting transdermal absorption of natural plant volatile oils that have been studied by many colleges and universities, to achieve a complex therapy of transdermal excipients-therapeutic drugs-aromatherapy relief.

[0009] The main principle of aromatherapy is: it reduces the levels of angiotensin (ANG), interleukin-6 (IL-6) and tumor necrosis factor (TNF-α), and significantly reverses depression and anxiety-like behaviors. Physiological regulation mechanism - olfactory pathway: The way in which aromatherapy works mainly depends on the olfactory pathway. The olfactory system is the earliest developed sensory organ in the human body. The specific process of aromatherapy is as follows: volatile aromatic molecules enter the lungs or nasal mucosa through inhalation, and then are absorbed into the capillary circulation or come into contact with the olfactory receptors distributed in the olfactory mucosa. The cilia on the surface of the olfactory mucosa are stimulated by the odor molecules, and the other end sends out olfactory nerve fibers, which are concentrated together to form olfactory filaments. These olfactory filaments pass through the sieve holes at the top of the nasal cavity and can directly bypass the blood-brain barrier and end at the olfactory bulb. The fibers sent out by the neurons of the olfactory bulb form the olfactory tract. The fibers of the olfactory tract pass through multiple neurons in the brain and finally transmit the odor information to the olfactory cortex deep in the brain, where the subjective recognition of smell is completed, so that the brain is "nourished" by aromatherapy from the outside to the inside, thereby producing corresponding physiological reactions, such as excitement, sedation, and soothing relaxation. As the part that is most closely connected to the brain, many documents have proved that aromatherapy can alleviate the symptoms of diseases such as AD, depression, and anxiety.

[0010] The main basis for the raw materials of volatile oils to promote transdermal absorption is that the stratum corneum, the outermost layer of the skin, is the main barrier to restrict the transdermal absorption of drugs, and most drugs are difficult to penetrate. The application of transdermal absorption enhancers (PE) is the most studied, most commonly used, and most convenient method to promote transdermal absorption. Terpene components are not only the main active ingredients of volatile oils of traditional Chinese medicine, but can also be used as PE. Among them, menthol, menthone, 1,8-cineole, etc. have been listed as GRAS (Generally recognized as safe) substances by the US FDA. According to statistics, among the 28 terpene components reported as PE, 23 are oxygenated monoterpenes, accounting for 82.14% of the total. It can be said that oxygenated monoterpenes are the mainstream of terpene PE, and their transdermal absorption promotion mechanism is mainly to interfere with the arrangement of stratum corneum lipids or to cause lipid extraction, thereby reducing the skin barrier function. Oxygenated monoterpenes are divided into two types: cyclic and chain. Some reports indicate that chain oxygenated monoterpenes may be better than cyclic ones because their chain structures are easier to penetrate into the intercellular lipid structure of the stratum corneum to exert their effects. However, some reports also indicate that cyclic oxygenated monoterpenes PE have a better transdermal absorption promoting effect than chain oxygenated monoterpenes. Therefore, the present invention selected 9 common oxygenated monoterpenoid components in volatile oils of traditional Chinese medicines, and systematically compared their transdermal absorption promoting effects and skin cell toxicity, in order to clarify the influence of oxygenated monoterpenoid structure on its application as PE.

[0011] In addition, considering the dramatic changes in the prices of Chinese medicinal materials in recent years, the cost of extracting volatile oils from Chinese medicines has also increased accordingly, and the treatment application area is wide, the cost of a single treatment is high, which is not conducive to use by the majority of consumers; the present invention optimizes the formula structure, such as adding base oil, adjusting the proportion of skin feel regulators, and replacing some precious Chinese medicines with more people-friendly varieties (or reducing the usage ratio), while ensuring the treatment effect, better serving the majority of consumers at all levels of the market.

[0012] In order to achieve the above object, the present invention adopts the following technical solution:

[0013] A plant-extracted volatile oil with fatigue-relieving and analgesic effects, comprising the following components in parts by weight:

[0014] 5-15 parts of pepper oil, 1-5 parts of pepper oil, 5-15 parts of camphor bark oil, 15-40 parts of camphor leaf oil, 10-40 parts of eucalyptus oil, 5-10 parts of peppermint oil, 15-30 parts of peppermint oil, 15-30 parts of ginger oil, 20-40 parts of clove bud oil, 5-15 parts of clove leaf oil, 15-30 parts of cinnamon oil, 10-20 parts of cassia oil, 5-10 parts of fennel oil, 15-30 parts of star anise oil 0 parts, 20-40 parts of clove basil oil, 10-20 parts of wormwood oil, 20-50 parts of thyme oil, 10-20 parts of linalool thyme oil, 10-20 parts of patchouli oil, 10-20 parts of nutmeg oil, 10-15 parts of notopterygium oil, 1-5 parts of angelica oil, 1-5 parts of lycopodiella oil, 1-10 parts of eucommia oil, 10-20 parts of dipsaci oil, 10-15 parts of stigma oil, 10-20 parts of saposhnikovia oil, 1-5 parts of perilla leaf oil, 1-5 parts of magnolia flower oil, 1-5 parts of calamus oil, 5-10 parts of ginkgo leaf oil, 10-20 parts of tangerine peel oil, 1-5 parts of rose oil, 10-20 parts of rhodiola rosea oil, 1-5 parts of ginseng oil, 10-20 parts of atractylodes oil, 10-20 parts of magnolia oil, 10-20 parts of chuanxiong oil, 1-5 parts of safflower oil, 0.5-3 parts of saffron oil, 5-1 parts of angelica oil 0 parts, 1-5 parts of angelica oil, 5-10 parts of salvia oil, 1-5 parts of frankincense oil, 5-10 parts of myrrh oil, 2-5 parts of benzoin oil, 1-5 parts of storax oil, 1-5 parts of costus root oil, 1-5 parts of cyperus oil, 0.5-5 parts of agarwood oil, 1-5 parts of sandalwood oil, 1-5 parts of dalbergia oil, 10-20 parts of menthol, 20-40 parts of ethanol, 5-10 parts of vitamin E oil and 50-100 parts of grape seed oil.

[0015] Grapeseed oil is used as a base oil, and combined with traditional Chinese medicine, physical therapy techniques and equipment, it can lubricate the skin. In addition to being used directly for massaging the skin, base oil is also a base oil for diluting essential oils.

[0016] The original aroma of the drug cannot be used as a single aromatherapy essential oil. After the proportion of the base oil is adjusted, it will evaporate into the smell of ordinary fruit oil.

[0017] Atractylodes macrocephala oil / essential oil, Atractylodes macrocephala rhizome extract, stem of Asteraceae plant, using supercritical CO2 extraction method. After extraction, it is a brown-red oily liquid with good liquid fluidity; the functions are: tonifying qi and strengthening the spleen, drying dampness and promoting diuresis, consolidating the exterior and stopping sweating, and calming the fetus; Chinese medicine mainly treats: fatigue and fatigue due to spleen and stomach qi deficiency, loss of appetite, abdominal distension, loose stools, even edema, urination difficulties, dampness and pain, spontaneous sweating due to exterior deficiency, and restless fetal movement. Modern research on this product also has the effect of improving memory; regulatory level: food, health products (supplies), cosmetics, and medicines can add raw materials. Different embodiments, about 10 to 20 parts, the minimum amount of ingredients can have an effect, and the number of parts is adjusted only due to different regulatory levels, resulting in the influence of the proportion of other ingredients.

[0018] Atractylodes oil / essential oil, Atractylodes rhizome extract, the rhizome of Asteraceae, is extracted by supercritical CO2. After extraction, it is a brown to brown viscous oily liquid with poor fluidity, only thick, and can be completely dissolved in lipid solvents. Efficacy: drying dampness and eliminating phlegm, lowering qi and removing fullness. The main treatments in traditional Chinese medicine are: used for dampness blocking the middle jiao, abdominal distension, diarrhea, edema, beriberi, rheumatism, cold, night blindness, and dizziness. Modern research on this product also has: effects on the central nervous system, and small doses are sedative. Regulatory level: raw materials can be added to cosmetics and medicines. Different embodiments, about 10 to 20 parts, the minimum amount of ingredients can have an effect, and the number of parts is adjusted only due to different regulatory levels, resulting in the influence of the proportion of other ingredients.

[0019] Magnolia officinalis oil / essential oil, Magnolia officinalis bark extract, dried bark, root bark and branch bark of Magnolia officinalis or Magnolia officinalis of the Magnoliaceae family, extracted by supercritical CO2. After extraction, it is a brown-yellow to brown-red viscous oily liquid with poor fluidity, only thick, and can be completely dissolved in lipid solvents. The effects are: drying dampness and eliminating phlegm, lowering qi and removing fullness. The main treatments in traditional Chinese medicine are: dampness stagnation and injury to the middle, abdominal distension and vomiting and diarrhea, food accumulation and qi stagnation, abdominal distension and constipation, phlegm and cough. Modern research on this product also has the effects of anti-inflammatory and analgesic, muscle relaxation, and relaxation of vascular smooth muscle. Regulatory level: raw materials can be added to cosmetics and medicines. Different embodiments, about 10-20 parts, the minimum amount of ingredients can have an effect, and the number of parts is adjusted only due to different regulatory levels, resulting in the influence of the proportion of other ingredients.

[0020] Chuanxiong oil / essential oil, LigUSTICUM CHUANXIONG extract, stem of Umbelliferae plant, extracted by supercritical CO2. After extraction, it is a brown-red oily liquid with good liquid fluidity; the function is to promote blood circulation and qi, dispel wind and relieve pain. The main treatments in traditional Chinese medicine are: used for chest pain, chest and flank pain, swelling and pain due to falls, irregular menstruation, amenorrhea, dysmenorrhea, headache, rheumatic pain. Modern research on this product also has the effects of anti-inflammatory, antipyretic, and sedative. Regulatory level: raw materials can be added to health products (supplies), cosmetics, and medicines. Different embodiments, about 10 to 20 parts, the minimum amount of ingredients can have an effect, and the number of parts is adjusted only due to different regulatory levels, which affects the proportion of other ingredients.

[0021] Safflower oil / essential oil, safflower (CARTHAMUS TINCTORIUS) flower extract, the flower of the Asteraceae plant, is extracted by supercritical CO2. After extraction, it is a yellow to light yellow oily liquid with good liquid fluidity; the effects are: promoting blood circulation and menstruation, removing blood stasis and relieving pain. The main treatments in traditional Chinese medicine are: used for blood stasis and dysmenorrhea, amenorrhea, postpartum lochia, abdominal pain due to stasis, lumps and masses, chest pain, bruises and swelling, and pain caused by stasis in the flanks and abdomen. Modern research on this product also has the following effects: treatment of frostbite, analgesic effect, and effect on the nervous system. Regulatory level: raw materials can be added to health products (supplies), cosmetics, and medicines. Different embodiments, about 1 to 5 parts, the minimum amount of ingredients can have an effect, and the number of parts is adjusted only due to different regulatory levels, resulting in the influence of the proportion of other ingredients.

[0022] Saffron oil / essential oil, Crocus SATIVUS flower oil or Crocus SATIVUS extract, the flower of the Iridaceae plant, is extracted by supercritical CO2; after extraction, it is a yellow to light yellow oily liquid with good liquid fluidity; the function is to promote blood circulation and remove blood stasis, and to relieve depression and relieve knots. Indications in Chinese medicine: Treat depression, chest tightness, vomiting blood, typhoid fever, panic, amenorrhea, abdominal pain due to postpartum blood stasis, and swelling and pain due to falls. Regulatory level: Raw materials can be added to food, health products (articles), cosmetics, and medicines. Since the food standard is only used as a flavoring agent, the implementation plan needs to adjust the dosage. Different embodiments, about 0.5 to 3 parts, the minimum amount of ingredients can have an effect, and the number of parts is adjusted only due to different regulatory levels, resulting in the influence of the proportion of other ingredients.

[0023] Angelica oil / essential oil, Angelica (ANGELICA POLYMORPHASINENSIS) root extract, the root of the Umbelliferae plant, using supercritical CO2 extraction method. After extraction, it is a brown-red viscous oily liquid with poor fluidity, only thick, and can be completely dissolved in lipid solvents. The effects are: nourishing blood and activating blood circulation, regulating menstruation and relieving pain, moistening the intestines and relieving constipation. The main indications in traditional Chinese medicine are: used for blood deficiency and chlorosis, dizziness and palpitations, irregular menstruation, amenorrhea and dysmenorrhea, abdominal pain due to deficiency and cold, rheumatic arthralgia, traumatic injuries, and dry intestines and constipation. Regulatory level: Food, health products (articles), cosmetics, and medicines can add raw materials. Different embodiments, about 5 to 10 parts, the minimum amount of ingredients can have an effect, and the number of parts is adjusted only due to different regulatory levels, which leads to the influence of the proportion of other ingredients.

[0024] Angelica dahurica oil / essential oil, Angelica dahurica extract is used in medicine (can also be used in food), and Angelica keiskei extract is mainly used in health care products and cosmetics. Both belong to the roots of Umbelliferae plants. The regulatory adjustment takes safety into consideration and adopts supercritical CO2 extraction. After extraction, it is a brown-yellow or brown-red viscous oily liquid. The fluidity of the fluid is poor, only thick, and can be completely dissolved in lipid solvents. The effects are: drying dampness and strengthening the spleen, dispelling wind and cold, and improving eyesight. The main treatments of traditional Chinese medicine are: used for blood deficiency and sallowness, irregular menstruation, spontaneous sweating, night sweats, flank pain, abdominal pain, and limb cramps. Modern research on this product also has the effects of anti-inflammatory, antipyretic and analgesic, antispasmodic, antihypertensive, and anti-radiation. Regulatory level: Food, health care products (supplies), cosmetics, and medicines can add raw materials. Different embodiments, about 1 to 5 parts, the minimum amount of ingredients can have an effect, and the number of parts is adjusted only due to different regulatory levels, resulting in the influence of the proportion of other ingredients.

[0025] Styrax oil / essential oil, Styrax (LIQUIDAMBARORIENTALIS) extract, resin of Hamamelidaceae plant, extracted by supercritical CO2. After extraction, it is a brown to dark brown oily liquid with good liquid fluidity; the effects are: opening the mind, eliminating filth, and relieving pain. The main indications in Chinese medicine are: used for stroke, phlegm, sudden fainting, chest pain, cold pain in the chest and abdomen, and epilepsy. Regulatory level: Raw materials can be added to cosmetics and medicines. Different embodiments, about 1 to 5 parts, the minimum amount of ingredients can have an effect, and the number of parts is adjusted only due to different regulatory levels, which affects the proportion of other ingredients.

[0026] Costus root oil / essential oil, SAUSSUREA LAPPA root extract, the root of Asteraceae plant, extracted by supercritical CO2. After extraction, it is a brown-red oily liquid with good liquid fluidity; the effects are: promoting blood circulation and relieving pain, strengthening the spleen and promoting digestion. The main indications in Chinese medicine are: used for distension and pain in the chest and flanks, abdomen, heaviness after diarrhea, indigestion, and loss of appetite. Regulatory level: raw materials can be added to health products (supplies), cosmetics, and medicines. Different embodiments, about 1 to 5 portions, the minimum amount of ingredients can have an effect, and the number of portions is adjusted only due to different regulatory levels, which affects the proportion of other ingredients.

[0027] Cyperus rotundus oil / essential oil, Cyperus rotundus root extract, the root of the sedge family, extracted by supercritical CO2. After extraction, it is a brown-yellow oily liquid with good liquid fluidity; the effects are: soothing the liver and regulating qi, regulating menstruation and relieving pain. The main treatments in traditional Chinese medicine are: chest and flank fullness, abdominal distension and pain, abdominal pain due to cold hernia, abdominal pain during menstruation, breast pain, amenorrhea, soothing the liver and relieving depression, promoting qi and relieving pain. Regulatory level: raw materials can be added to health products (supplies), cosmetics, and medicines. Different embodiments, about 1 to 5 parts, the minimum amount of ingredients can have an effect, and the number of parts is adjusted only due to different regulatory levels, which affects the proportion of other ingredients.

[0028] Agarwood oil / essential oil, Aquilaria (AQUILARIAE LIGNUMRESINATUM) extract, wood block of Thymelaeaceae plant, extracted by supercritical CO2. After extraction, it is a light yellow to light yellow brown oily liquid with good liquid fluidity; the effects are: promoting qi and relieving pain, warming the middle and stopping vomiting, and relieving asthma. The main treatments in traditional Chinese medicine are: used for chest and abdominal distension and pain, chills, vomiting and hiccups, and kidney deficiency, qi reversal and asthma. Modern research on this product also shows: antibacterial and analgesic effects. Regulatory level: Raw materials can be added to cosmetics and medicines. Different embodiments, about 0.5 to 5 parts, the minimum amount of ingredients can have an effect, and the number of parts is adjusted only due to different regulatory levels, which affects the proportion of other ingredients.

[0029] Sandalwood oil / essential oil, sandalwood (SANTALUM ALBUM) oil, wood blocks of Santalaceae plants, extracted by supercritical CO2. After extraction, it is a golden oily liquid with good liquid fluidity; the effects are: moisture absorption and sore retention, cough and sore throat, diuresis and stranguria, sedation and tranquilization, and pain relief. The main treatments in Chinese medicine are: relieving stress, anxiety, and nervous tension. Modern research on this product also shows: anti-depressant, anti-inflammatory, antispasmodic, and sedative effects. Regulatory level: Raw materials can be added to cosmetics and medicines. Different embodiments, about 1 to 5 parts, the minimum amount of ingredients can have an effect, and the number of parts is adjusted only due to different regulatory levels, which affects the proportion of other ingredients.

[0030] Zanthoxylum bungeanum oil / essential oil, Zanthoxylum bungeanum fruit extract, the fruit of Rutaceae, extracted by supercritical CO2; after extraction, it is a brown-yellow oily liquid with good liquid fluidity; functions: warming the middle and relieving pain, killing insects and relieving itching; Chinese medicine treatment: used for spleen and stomach deficiency and cold, abdominal pain, vomiting and diarrhea, abdominal pain caused by roundworms, toothache caused by caries, vaginal itching and leucorrhea, and eczema itching; modern research on this product also has: antioxidant and antibacterial effects, so this product is also used as a natural antioxidant and preservative. Regulatory level: raw materials can be added to food, health products (supplies), cosmetics, and medicines. Different embodiments, about 5 to 15 parts, the minimum amount of ingredients can have an effect, and the number of parts is adjusted only due to different regulatory levels, which affects the proportion of other ingredients.

[0031] Pepper oil / essential oil, pepper (PIPERNIGRUM) fruit oil, dried nearly mature or mature fruits of pepper, a plant of the Piperaceae family, extracted by supercritical CO2. After extraction, it is a light yellow to light yellow-brown oily liquid with good liquid fluidity; the effects are: warming the middle and dispersing cold, lowering qi, and eliminating phlegm. The main treatments in traditional Chinese medicine are: treating cold phlegm and food accumulation, cold pain in the abdomen, nausea, vomiting of clear water, diarrhea, and cold dysentery. And detoxification of food. Stomach cold vomiting, abdominal pain and diarrhea, loss of appetite, epilepsy and sputum. Modern research on this product also has: anticonvulsant, antidepressant, sedative, analgesic, anti-inflammatory effects, and effects on smooth muscle. Regulatory level: Food, health products (supplies), cosmetics, and medicines can add raw materials. Different embodiments, about 1 to 5 parts, the minimum amount of ingredients can have an effect, and the number of parts is adjusted only due to different regulatory levels, resulting in the influence of the proportion of other ingredients.

[0032] Benzoin oil / essential oil, benzoin (STYRAX BENZOIN) resin extract, resin of Styraxaceae plant, extracted by supercritical CO2. After extraction, it is a brown-red oily liquid with good liquid fluidity; the effects are: refreshing, promoting qi and blood circulation, and relieving pain. The main treatments in Chinese medicine are: used for stroke, phlegm, qi stagnation, coma, heart and abdomen pain, postpartum blood loss, and infantile convulsions. Regulatory level: raw materials can be added to cosmetics and medicines. Different embodiments, about 2 to 5 parts, the minimum amount of ingredients can have an effect, and the number of parts is adjusted only due to different regulatory levels, which affects the proportion of other ingredients.

[0033] Rhodiola rosea oil / essential oil, Rhodiola rosea (RHODIOLA ROSEA) extract or Rhodiola rosea (RHODIOLA ROSEA) root extract, the root of the Rhodiola rosea plant, using a steam distillation extraction process; after extraction, it is a colorless to light yellow oily liquid with good liquid fluidity; the effects are: replenishing qi and clearing the lungs; improving intelligence and nourishing the heart; astringing and stopping bleeding; dispersing blood stasis and reducing swelling. The main treatments in traditional Chinese medicine are: weak body due to qi deficiency; fear of cold after illness; shortness of breath and fatigue; cough due to lung heat; hemoptysis; leucorrhea and diarrhea; trauma; burns; neurosis; altitude sickness, etc. Regulatory level: raw materials can be added to health products (supplies), cosmetics, and medicines. Different embodiments, about 10 to 20 parts, the minimum amount of ingredients can have an effect, and the number of parts is adjusted only due to different regulatory levels, which leads to the influence of the proportion of other ingredients.

[0034] Ginseng oil / essential oil, ginseng (PANAX GINSENG) root extract, the root of the genus Panax of the Araliaceae family, is extracted by steam distillation; after extraction, it is a light yellow oily liquid with good liquid fluidity; the effects are: greatly replenishing vital energy, consolidating the body and producing body fluid, and calming the mind. The main treatments in traditional Chinese medicine are: treating fatigue due to overwork, poor appetite, fatigue, nausea and vomiting, loose stools, weak cough and shortness of breath, spontaneous sweating and sudden loss of body fluids, palpitations, forgetfulness, dizziness and headache, impotence, frequent urination, thirst, metrorrhagia in women, slow fright in children, and long-term deficiency, all signs of insufficient qi, blood and body fluids. Regulatory level: raw materials can be added to food, health products (supplies), cosmetics, and medicines. Different embodiments, about 1 to 5 parts, the minimum amount of ingredients can have an effect, and the number of parts is adjusted only due to different regulatory levels, resulting in the influence of the proportion of other ingredients.

[0035] Camphor bark oil / essential oil, camphor tree (CINNAMOMUM CAMPHORA) bark oil, the bark of camphor tree of the Lauraceae family, is extracted by steam distillation; after extraction, it is a colorless to light yellow oily liquid with good liquid fluidity; the effects are: clearing the orifices and eliminating filth, warming the middle and relieving pain, and promoting dampness and killing insects. The main indications in Chinese medicine are: used for vomiting and diarrhea caused by cold and dampness, stomach and abdominal pain, athlete's foot, traumatic injuries, scabies, and pain caused by caries. Regulatory level: raw materials can be added to cosmetics and medicines. Different embodiments, about 5-15 parts, the minimum amount of ingredients can have an effect, and the number of parts is adjusted only due to different regulatory levels, which leads to the influence of the proportion of other ingredients.

[0036] Camphor leaf oil / essential oil, camphor (CINNAMOMUM CAMPHORA) leaf extract, leaves of camphor, using steam distillation extraction process; after extraction, it is a light yellow oily liquid with good liquid fluidity; the function and indication of this product are consistent with camphor bark, and the purpose of using this product and the above-mentioned camphor bark oil is mainly to replace the camphor of drug standards. Camphor is a granular material made by distillation and refining of the roots, stems, branches and leaves of camphor Cinnamomum camphora (L.) Presl. of the genus camphor ...

[0037] Eucalyptus (leaf) oil / essential oil, eucalyptus globulus leaf oil or eucalyptus globulus oil, pharmacopoeia name: eucalyptus oil, leaves of eucalyptus globulus of the Myrtaceae family, extracted by steam distillation; after extraction, it is a colorless to light yellow oily liquid with good liquid fluidity; the effects are: dispelling wind and relieving exterior symptoms, clearing away heat and detoxifying, resolving phlegm and regulating qi, killing insects and relieving itching. The main treatments in traditional Chinese medicine are: colds, high fever and cough, whooping cough, abdominal distension and pain, diarrhea, dysentery, hook, filariasis, malaria, rheumatism, eczema, scabies, burns and scalds, and traumatic bleeding. Regulatory level: Raw materials can be added to cosmetics and medicines. Different embodiments, about 10 to 40 parts, the minimum amount of ingredients can have an effect, and the number of parts is adjusted only due to different regulatory levels, resulting in the influence of the proportion of other ingredients.

[0038] Patchouli oil / essential oil, patchouli (POGOSTEMON CABLIN) oil, the whole plant of the Lamiaceae plant, is extracted by steam distillation; after extraction, it is a brown-red oily liquid with good liquid fluidity; the effects are: aromatic and turbid, soothing and stopping vomiting, and relieving heat. The main treatments in traditional Chinese medicine are: used for dampness and turbidity blocking the middle, abdominal distension and vomiting, symptoms of summer heat and dampness, the initial onset of damp-heat, fever and fatigue, chest tightness and discomfort, cold and dampness, abdominal pain, vomiting and diarrhea, and vomiting. Modern research on this product also has: antiseptic and antibacterial effects. Regulatory level: raw materials can be added to food, health products (supplies), cosmetics, and medicines. Different embodiments, about 10 to 20 parts, the minimum amount of ingredients can have an effect, and the number of parts is adjusted only due to different regulatory levels, which leads to the influence of the proportion of other ingredients.

[0039] Nutmeg oil / essential oil, nutmeg (MYRISTICA FRAGRANS) kernel oil, the fruit of the ginger plant, is extracted by steam distillation; after extraction, it is a colorless to light yellow oily liquid with good liquid fluidity; the effects are: removing dampness and promoting qi, warming the middle and stopping vomiting, and stimulating appetite and digestion. The main indications in Chinese medicine are: used for dampness and turbidity blocking the middle, loss of appetite, chest and abdominal distension and pain, indigestion, initial dampness and heat, chest tightness without hunger, cold and dampness and vomiting. Regulatory level: raw materials can be added to food, health products (articles), cosmetics, and medicines. Different embodiments, about 10 to 20 parts, the minimum amount of ingredients can have an effect, and the number of parts is adjusted only due to different regulatory levels, which affects the proportion of other ingredients.

[0040] Salvia miltiorrhiza oil / essential oil, Salvia miltiorrhiza (SALVIA MILTIORRHIZA) root extract, the root of the Lamiaceae plant, is extracted by steam distillation; after extraction, it is a light brown to light yellow green oily liquid with good liquid fluidity; the effects are: promoting blood circulation and removing blood stasis, relieving pain through menstruation, clearing the heart and eliminating vexation, cooling blood and eliminating carbuncle. The main indications in traditional Chinese medicine are: used for chest pain, abdominal pain, dysmenorrhea, heat pain, irritability and insomnia, irregular menstruation, dysmenorrhea and amenorrhea, and traumatic injuries. Regulatory level: raw materials can be added to health products (supplies), cosmetics, and medicines. Different embodiments, about 5 to 10 parts, the minimum amount of ingredients can have an effect, and the number of parts is adjusted only due to different regulatory levels, which affects the proportion of other ingredients.

[0041] Frankincense oil / essential oil, frankincense (BOSWELLIA CARTERII) oil, resin of olive family, extracted by steam distillation; after extraction, it is a colorless to light yellow oily liquid with good liquid fluidity; the effects are: promoting blood circulation and relieving pain, reducing swelling and promoting tissue regeneration. The main treatments in traditional Chinese medicine are: used for chest pain, heart pain, stomach pain, dysmenorrhea, amenorrhea, postpartum blood stasis, rheumatic pain. Modern research shows that this product also has the effects of calming the nerves, relieving pain and inflammation, and antibacterial. Regulatory level: Raw materials can be added to cosmetics and medicines. Different embodiments, about 1 to 5 parts, the minimum amount of ingredients can have an effect, and the number of parts is adjusted only due to different regulatory levels, which leads to the influence of the proportion of other ingredients.

[0042] Myrrh oil / essential oil, myrrh (COMMIPHORA MYRRHA) resin extract, dried resin of the olive plant genus genus genus genus, using supercritical CO2 extraction method. After extraction, it is a light yellow to light yellow brown oily liquid with poor liquid fluidity; the effects are: promoting blood circulation and relieving pain; reducing swelling and promoting tissue regeneration. The main treatments in traditional Chinese medicine are: chest and abdominal blood stasis and pain; dysmenorrhea; amenorrhea; symptoms; traumatic injuries; carbuncle ulcers; intestinal carbuncle; red and swollen eyes. Used for traumatic blood stasis and pain, carbuncle swelling and pain, chest and abdominal pain; external use to treat sores that do not heal for a long time. Modern research on this product also shows that the ingredients such as bisabolol contained in myrrh can effectively relieve the pain sensation in the human body and improve the pain tolerance of the human body. Regulatory level: raw materials can be added to health products (supplies), cosmetics, and medicines. Different embodiments, about 5 to 10 parts, the minimum amount of ingredients can have an effect, and the number of parts is adjusted only due to different regulatory levels, resulting in the influence of the proportion of other ingredients.

[0043] Peppermint oil / essential oil, Mentha Piperita oil, the whole plant of the Lamiaceae family, is extracted using a steam distillation process; after extraction, it is a light yellow oily liquid with good liquid fluidity; the medical effects of this product are consistent with those of peppermint oil / essential oil raw materials, and its main function when added alone is as a cooling agent or skin feel regulator. Regulatory level: Raw materials can be added to foods, health products (articles), cosmetics, and medicines. In different embodiments, about 5 to 10 portions, the minimum amount of ingredients can produce an effect, and the number of portions is adjusted only due to different regulatory levels, which affects the proportion of other ingredients.

[0044] Peppermint oil / essential oil, peppermint (MENTHA HAPLOCALYX) oil, leaves of Lamiaceae plants, extracted by steam distillation; after extraction, it is a light yellow oily liquid with good liquid fluidity; the effects are: evacuating wind-heat, clearing the head, relieving sore throat, clearing rashes, soothing the liver and promoting qi. The main treatments in Chinese medicine are: used for wind-heat colds, the initial onset of wind-heat, headaches, red eyes, throat paralysis, mouth sores, rubella, measles, and chest and flank distension. Modern research has shown that this product also has: analgesic, antipruritic, anti-irritant, and bactericidal effects. Regulatory level: raw materials can be added to food, health products (articles), cosmetics, and medicines. Different embodiments, about 15-30 parts, the minimum amount of ingredients can have an effect, and the number of parts is adjusted only due to different regulatory levels, which leads to the influence of the proportion of other ingredients.

[0045] Ginger oil / essential oil, ginger (ZINGIBER OFFICINALE) root oil, rhizome of ginger plant, extracted by steam distillation; after extraction, it is a light brown to light brown oily liquid with good liquid fluidity; the effects are: relieving cold, warming the middle and stopping vomiting, resolving phlegm and relieving cough. The main treatments in Chinese medicine are: used for colds, stomach cold vomiting, and cold phlegm cough. According to modern research, this product also has: antiemetic effect. Regulatory level: raw materials can be added to food, health products (supplies), cosmetics, and medicines. Different embodiments, about 15 to 30 parts, the minimum amount of ingredients can have an effect, and the number of parts is adjusted only due to different regulatory levels, which affects the proportion of other ingredients.

[0046] Perilla leaf oil / essential oil, Perilla (PERILLA OCYMOIDES) leaf extract, leaves of Lamiaceae plants, extracted by steam distillation; after extraction, it is a brown-yellow oily liquid with good liquid fluidity; the effects are: relieving exterior symptoms and dispelling cold, promoting qi and harmonizing the stomach. The main indications in Chinese medicine are: used for colds, coughs and nausea, vomiting during pregnancy, and fish and crab poisoning. Modern research has shown that this product also has the effects of: improving memory and promoting the growth of synapses in brain nerve cells. Regulatory level: Raw materials can be added to food, health products (supplies), cosmetics, and medicines. Different embodiments, about 1 to 5 portions, the minimum amount of ingredients can have an effect, and the number of portions is adjusted only due to different regulatory levels, which affects the proportion of other ingredients.

[0047] Magnolia flower oil / essential oil, MAGNOLIA BIONDII flower extract, the flower of the Magnoliaceae plant, is extracted by steam distillation; after extraction, it is a light yellow oily liquid with good liquid fluidity; the effects are: dispersing wind and cold, and clearing the nasal passages. The main treatments in Chinese medicine are: used for wind-cold headache, nasal congestion and runny nose, allergic rhinitis, and sinusitis. According to modern research, this product also has: local astringent and antibacterial, anti-inflammatory effects, analgesic effects, skeletal muscle relaxation effects, and anti-allergic effects. Regulatory level: Raw materials can be added to cosmetics and medicines. Raw materials can be added to cosmetics and medicines. Different embodiments, about 1 to 5 parts, the minimum amount of ingredients can have an effect, and the number of parts is adjusted only due to different regulatory levels, which affects the proportion of other ingredients.

[0048] Acorus calamus oil / essential oil, Acorus calamus (ACORUS CALAMUS) root extract, the root of the Araceae plant, is extracted by steam distillation; after extraction, it is a light yellow oily liquid with good liquid fluidity; the effects are: opening the mind and eliminating phlegm, refreshing the mind and improving intelligence, removing dampness and stimulating appetite. The main indications in Chinese medicine are: used for coma, epilepsy, forgetfulness and insomnia, tinnitus and deafness, abdominal distension without hunger, and dysentery. Regulatory level: Raw materials can be added to cosmetics and medicines. Different embodiments, about 1 to 5 parts, the minimum amount of ingredients can have an effect, and the number of parts is adjusted only due to different regulatory levels, which affects the proportion of other ingredients.

[0049] Ginkgo leaf oil / essential oil, Ginkgo (GINKGO BILOBA) leaf extract, leaves of Ginkgoaceae plants, extracted by steam distillation; after extraction, it is a colorless to light yellow oily liquid with good liquid fluidity; the effects are: benefiting the heart and lungs, removing dampness and stopping diarrhea. The main treatments in Chinese medicine are: treating chest tightness and heart pain, palpitations, phlegm and cough, diarrhea, and leucorrhea. Modern research has shown that this product also has the effect of improving microcirculation. Regulatory level: raw materials can be added to health products (supplies), cosmetics, and medicines. Different embodiments, about 5 to 10 parts, the minimum amount of ingredients can have an effect, and the number of parts is adjusted only due to different regulatory levels, which affects the proportion of other ingredients.

[0050] Tangerine peel oil / essential oil, citrus (CITRUS RETICULATA) peel extract, the peel of the Rutaceae plant, using steam distillation extraction process; after extraction, it is a colorless to light yellow oily liquid with good liquid fluidity; the effects are: regulating qi, regulating the middle, drying dampness, and resolving phlegm. The main treatments in Chinese medicine are: treating chest and abdominal distension, loss of appetite, vomiting, hiccups, coughing and sputum. It also relieves fish and crab poison. Regulatory level: raw materials can be added to food, health products (supplies), cosmetics, and medicines. Different embodiments, about 10 to 20 portions, the minimum amount of ingredients can have an effect, and the number of portions is adjusted only due to different regulatory levels, which affects the proportion of other ingredients.

[0051] Rose essential oil, Rosa damascena flower oil, the flower of the Rosaceae plant, is extracted by steam distillation; after extraction, it is a light yellow oily liquid with good liquid fluidity; the effects are: regulating qi and relieving depression, and harmonizing blood and dispersing blood stasis. The main treatments in traditional Chinese medicine are: treating liver and stomach qi pain, new and long-term wind arthralgia, vomiting blood and hemoptysis, irregular menstruation, leucorrhea, dysentery, breast abscess, and swelling. Dual-purpose ingredients, in addition to their efficacy in traditional Chinese medicine, are also used as flavoring agents / aromatics. Regulatory level: Raw materials can be added to food, health products (articles), cosmetics, and medicines. Different embodiments, about 1 to 5 parts, the minimum amount of ingredients can have an effect, and the number of parts is adjusted only due to different regulatory levels, which leads to the influence of the proportion of other ingredients.

[0052] Clove bud oil / essential oil, clove (EUGENIA CARYOPHYLLUS) bud oil, the buds of the Myrtaceae plant, are extracted by steam distillation; after extraction, it is a light yellow oily liquid with good liquid fluidity; the effects are: warming the middle and lowering adverse reactions, tonifying the kidney and assisting yang. The main indications in traditional Chinese medicine are: used for spleen and stomach deficiency and cold, hiccups and vomiting, poor appetite, vomiting and diarrhea, cold pain in the heart and abdomen, impotence due to kidney deficiency, and cold uterus. Modern research on this product also shows pharmacological effects such as antibacterial, analgesic, and inflammation control. Regulatory level: Raw materials can be added to food, health products (supplies), cosmetics, and medicines. Different embodiments, about 20 to 40 portions, the minimum amount of ingredients can have an effect, and the number of portions is adjusted only due to different regulatory levels, resulting in the influence of the proportion of other ingredients.

[0053] Clove leaf oil / essential oil, clove (EUGENIA CARYOPHYLLUS) leaf oil, the leaves of the Myrtaceae plant, extracted by steam distillation; after extraction, it is a light yellow oily liquid with good liquid fluidity; the efficacy is / the main treatment in traditional Chinese medicine is the same as that of clove bud oil. The purpose of adding clove bud oil and clove leaf oil at the same time is to ensure the content of chromones and flavonoids in cloves, the main ingredients for antibacterial and analgesic effects. Regulatory level: raw materials can be added to food, health products (supplies), cosmetics, and medicines. Different embodiments, about 5 to 15 parts, the minimum amount of ingredients can have an effect, and the adjustment of the number of parts is only due to different regulatory levels, which affects the proportion of other ingredients.

[0054] Cinnamon oil / essential oil, cinnamon (CINNAMOMUM CASSIA) extract or cinnamon (CINNAMOMUM CASSIA) bark extract, the bark of the Lauraceae plant, using steam distillation extraction process; after extraction, it is a light brown to light yellow-green oily liquid with good liquid fluidity; the effects are: replenishing fire and yang, guiding fire back to its origin, dispelling cold and relieving pain, and warming the meridians. The main treatments in traditional Chinese medicine are: used for impotence, cold palace, cold pain in the waist and knees, cold pain in the heart and abdomen, sweating, vomiting and diarrhea, cold hernia abdominal pain, dysmenorrhea, amenorrhea, cold and dampness, yin carbuncle flow, kidney deficiency and asthma, floating of virtual yang, dizziness and red eyes. According to modern research, this product also has the effects of sterilization, sedation, antipyretic, anticonvulsant, etc. Regulatory level: food, health products (supplies), cosmetics, and medicines can add raw materials. Different embodiments, about 15 to 30 parts, the minimum amount of ingredients can have an effect, and the number of parts is adjusted only due to different regulatory levels, resulting in the influence of the proportion of other ingredients.

[0055] Cinnamon twig oil / essential oil, cinnamon bark extract or cinnamon bark extract are all Lauraceae plants. Cinnamon twig refers specifically to the dried tender branches of Lauraceae plants. It is mainly distinguished from cinnamon as tender branches and single bark. The cosmetic level does not distinguish specific terms. The maximum content value is managed together with cinnamon (that is, the content ratio is combined with the total content of cinnamon). After extraction, it is a light brown to bright yellow oily liquid with good liquid fluidity; the effects are: releasing and relieving muscles, warming the meridians and unblocking the meridians, assisting yang and transforming qi, and balancing and descending qi. The main treatments in traditional Chinese medicine are: colds caused by wind and cold, various pains caused by cold blood stagnation, phlegm and fluid retention, water retention syndrome, and palpitations. According to modern research, this product also has the effects of antipyretic, analgesic, sedative and anticonvulsant; it has obvious anti-inflammatory effects. Regulatory level: food, health products (supplies), cosmetics, and medicines can add raw materials. In different embodiments, about 10 to 20 portions are the minimum amount of ingredients that are effective, and the adjustment in the number of portions is only due to the different regulatory levels, which affects the proportion of other ingredients.

[0056] Fennel oil / essential oil, fennel (FOENICULUM VULGARE) oil, seeds of Umbelliferae plants, extracted by steam distillation; after extraction, it is a light yellow oily liquid with good liquid fluidity; the effects are: dispelling cold and relieving pain, regulating qi and harmonizing the stomach. The main treatments in traditional Chinese medicine are: used for abdominal pain caused by cold hernia, testicular prolapse, dysmenorrhea, cold pain in the lower abdomen, abdominal distension and pain, and vomiting blood due to poor appetite. Modern research has shown that this product also has: antibacterial effects, especially for fungal spores, avian tuberculosis Mycobacterium, and Staphylococcus aureus. Regulatory level: Raw materials can be added to food, health products (supplies), cosmetics, and medicines. Different embodiments, about 5 to 10 parts, the minimum amount of ingredients can have an effect, and the number of parts is adjusted only due to different regulatory levels, which affects the proportion of other ingredients.

[0057] Star anise oil / essential oil, star anise (ILLICIUM VERUM) oil or star anise (ILLICIUM VERUM) fruit / seed oil, seeds of Magnoliaceae plants, extracted by steam distillation; after extraction, it is a light yellow oily liquid with good liquid fluidity; the effects are: warming yang and dispelling cold, regulating the stomach, regulating qi and relieving pain. The main treatments in traditional Chinese medicine are: used for abdominal pain caused by cold hernia, cold pain in the waist and knees, vomiting due to stomach cold, and pain in the epigastric region. Modern research has shown that this product also has: antibacterial effect, analgesic effect, and antioxidant effect. Regulatory level: raw materials can be added to food, health products (supplies), cosmetics, and medicines. Different embodiments, about 15 to 30 parts, the minimum amount of ingredients can have an effect, and the number of parts is adjusted only due to different regulatory levels, which affects the proportion of other ingredients.

[0058] Clove basil oil / essential oil, basil (OCIMUM BASILICUM) oil, pharmacopoeia name: Clove basil oil, the whole plant of the Lamiaceae family, extracted by steam distillation; after extraction, it is a light yellow oily liquid with good liquid fluidity; the effects are: local analgesic, antiseptic. The main treatments in Chinese medicine are: used to treat caries, etc. Dual-purpose ingredient, in addition to local analgesia, it is also used as a natural preservative. Regulatory level: raw materials can be added to cosmetics and medicines. Different embodiments, about 20 to 40 parts, the minimum amount of ingredients can have an effect, and the number of parts is adjusted only due to different regulatory levels, which affects the proportion of other ingredients.

[0059] Artemisia argyi leaf oil / essential oil, Artemisia aargyi leaf extract, the leaves of the Asteraceae plant Artemisia argyi, are extracted by steam distillation; after extraction, it is a light yellow oily liquid with good liquid fluidity; the effects are: regulating qi and blood, expelling cold and dampness; warming the meridians, stopping bleeding, and calming the fetus. The main treatments in traditional Chinese medicine are: cold pain in the heart and abdomen, diarrhea and cramps, chronic dysentery, vomiting and bleeding, bleeding, irregular menstruation, metrorrhagia, leucorrhea, fetal movement disorder, carbuncle, scabies. According to modern research, this product also has the effects of anti-inflammatory, analgesic, and anti-allergic. Regulatory level: raw materials can be added to food, health products (supplies), cosmetics, and medicines. Different embodiments, about 10 to 20 parts, the minimum amount of ingredients can have an effect, and the number of parts is adjusted only due to different regulatory levels, resulting in the influence of the proportion of other ingredients.

[0060] Qianghuo oil / essential oil, Qianghuo (NOTOPTERYGIUM INCISUM) extract, roots and rhizomes of Qianghuo plants of the Umbelliferae family. Use steam distillation extraction process; after extraction, it is a light yellow oily liquid with good liquid fluidity; the effects are: dispersing superficial cold; removing rheumatism; benefiting joints; relieving pain. The main treatments in traditional Chinese medicine are: exogenous wind-cold; headache without sweating; oil-printed cold and dampness; edema; sores and swelling. Modern research has shown that this product also has: anti-inflammatory and analgesic effects. Regulatory level: raw materials can be added to health products (supplies), cosmetics, and medicines. Different embodiments, about 10 to 15 parts, the minimum amount of ingredients can have an effect, and the number of parts is adjusted only due to different regulatory levels, which affects the proportion of other ingredients.

[0061] Angelica Pubescens oil / essential oil, Angelica Pubescens extract, the root of the plant Angelica sinensis, using supercritical CO2 extraction method. After extraction, it is a light yellow to light yellow brown oily liquid with good liquid fluidity; the effects are: dispelling wind and dampness; dispelling cold and relieving pain. The main treatments in traditional Chinese medicine are: headache caused by wind-cold and dampness; pain in the waist and knees; headache and toothache, Shaoyin Fufeng headache, cramps in the hands and feet, and chronic tracheitis. Modern research on this product also has: sedative, hypnotic, analgesic, and anti-inflammatory effects. Regulatory level: raw materials can be added to health products (supplies), cosmetics, and medicines. Since this product belongs to the classic Qianghuo-Douhuo formula, it is a necessary ingredient from the perspective of traditional Chinese medicine, but this product contains certain substances that cause photosensitivity dermatitis (psoralen derivatives), and the content of each embodiment does not exceed 0.2%. Different embodiments, about 1 to 5 parts, the minimum amount of ingredients can have an effect, and the number of parts is adjusted only due to different regulatory levels, resulting in the influence of the proportion of other ingredients. Since this product belongs to the classic Qianghuo-Duhuo formula, it is a necessary ingredient from the perspective of traditional Chinese medicine. However, this product contains certain substances that cause photosensitivity dermatitis (psoralen derivatives), and the content in each example does not exceed 0.2%.

[0062] Lycopodium clavatum oil / essential oil, Lycopodium clavatum / European Lycopodium clavatum extract, the dried whole herb of Lycopodium clavatum, a plant of the Lycopodiaceae family. Use steam distillation extraction process; after extraction, it is a light yellow oily liquid with good liquid fluidity; the effects are: dispelling wind and cold, removing dampness and swelling, relaxing muscles and activating blood circulation. The main treatments in traditional Chinese medicine are: wind-cold and dampness, joint pain, skin numbness, weak limbs, edema, and external use for the treatment of swelling and pain caused by falls and sprains. Regulatory level: raw materials can be added to health products (supplies), cosmetics, and medicines. Different embodiments, about 1 to 5 parts, the minimum amount of ingredients can have an effect, and the number of parts is adjusted only due to different regulatory levels, which leads to the influence of the proportion of other ingredients.

[0063] Dalbergia odorifera oil / essential oil, Dalbergia odorifera extract, the dried heartwood of the trunk and root of the leguminous plant Dalbergia odorifera, extracted by supercritical CO2. After extraction, it is a light yellow to light yellow brown oily liquid with good liquid fluidity; the effects are: promoting qi and blood circulation, stopping bleeding and relieving pain, lowering qi, and warding off filth. The main treatments in traditional Chinese medicine are: chest and flank pain, traumatic injury, liver depression and flank pain, chest pain, traumatic bleeding, cold hernia pain or vomiting and abdominal pain caused by internal obstruction of filth. Modern research on this product also shows that Dalbergia odorifera mainly contains volatile oils and flavonoids, which have sedative, analgesic and anticonvulsant effects. Regulatory level: raw materials can be added to health products (supplies), cosmetics, and medicines. Different embodiments, about 1 to 5 parts, the minimum amount of ingredients can have an effect, and the number of parts is adjusted only due to different regulatory levels, resulting in the influence of the proportion of other ingredients.

[0064] Eucommia oil / essential oil, Eucommia ulmoides bark extract, bark of Eucommia ulmoides, Eucommia ulmoides plant, extracted by steam distillation; after extraction, it is a light yellow oily liquid with good liquid fluidity; the effects are: nourishing the liver and kidneys, strengthening the tendons and bones, and calming the fetus. The main treatments in traditional Chinese medicine are: low back pain due to kidney deficiency, weak tendons and bones, sore waist and knees, impotence, frequent urination, residual urine, rheumatic pain, fetal movement disorder, and habitual abortion. Regulatory level: raw materials can be added to food, health products (supplies), cosmetics, and medicines. Different embodiments, about 1 to 10 parts, the minimum amount of ingredients can have an effect, and the number of parts is adjusted only due to different regulatory levels, which leads to the influence of the proportion of other ingredients.

[0065] Dipsacus sylvestris oil / essential oil, wild Dipsacus sylvestris extract, the root of the Dipsacus sylvestris plant of the Dipsacus sylvestris family, is extracted by steam distillation; after extraction, it is a light yellow oily liquid with good liquid fluidity; the effects are: nourishing the liver and kidneys, strengthening the tendons and bones, regulating blood vessels, healing fractures, and stopping metrorrhagia. The main treatments in traditional Chinese medicine are: sore back and waist; paralysis of limbs; trauma from falls, damaged tendons and broken bones, bleeding during fetal movement, metrorrhagia, spermatorrhea, leucorrhea, carbuncle and swelling. Regulatory level: raw materials can be added to food, health products (articles), cosmetics, and medicines. Different embodiments, about 10-20 parts, the minimum amount of ingredients can have an effect, and the number of parts is adjusted only due to different regulatory levels, which leads to the influence of the proportion of other ingredients.

[0066] Elderberry oil / essential oil, elderberry / western elderberry (SAMBUCUS NIGRA) oil, tall herb or subshrub of the genus Sambucus in the family Sambucus, extracted by steam distillation; after extraction, it is a light yellow oily liquid with good liquid fluidity; the effects are: dispelling wind and dampness; promoting blood circulation; stopping bleeding. The main treatments in traditional Chinese medicine are: rheumatism and pain; gout; osteomalacia; acute and chronic nephritis; rubella; traumatic injuries; fracture swelling and pain; traumatic bleeding. Regulatory level: raw materials can be added to food, health products (articles), cosmetics, and medicines. Different embodiments, about 10 to 15 parts, the minimum amount of ingredients can have an effect, and the number of parts is adjusted only due to different regulatory levels, which affects the proportion of other ingredients.

[0067] Saposhnikovia divaricata root extract, root of Saposhnikovia divaricata, Umbelliferae plant, using supercritical CO2 extraction method. After extraction, it is a light yellow to light yellow brown oily liquid with good liquid fluidity; the effects are: dispelling wind and relieving symptoms, removing dampness and relieving pain, and stopping spasms. Chinese medicine mainly treats: colds and headaches, rheumatic pain, urticaria itching, tetanus, rheumatic pain, tetanus opisthotonos, trismus, convulsions and spasms. Modern research on this product also has the effects of antipyretic, cooling, analgesic, sedative and anticonvulsant, antibacterial, anti-inflammatory, antihistamine, and enhancing immune system function; regulatory level: food, health products (supplies), cosmetics, and medicines can add raw materials. Different embodiments, about 10 to 20 parts, the minimum amount of ingredients can have an effect, and the number of parts is adjusted only due to different regulatory levels, resulting in the influence of the proportion of other ingredients.

[0068] Menthol / menthol, menthol (MENTHOL) / menthol (MENTHOLUM), due to different extraction equipment, the steam distillation extraction process only extracts the volatile oil components in the plant mint, and its extraction wastewater-water-soluble substances are menthols (menthol dew). Even if it is distilled twice, the bacteria-colony count in the circulating water does not meet the most stringent regulations (pharmaceutical grade requirements), so this product uses purchased raw materials, that is, separately purified menthol crystals. The medical effect is consistent with that of peppermint oil. The main function of adding it alone is: a cooling agent or a skin feel regulator. Regulatory level: raw materials can be added to food, health products (articles), cosmetics, and medicines. Because it is a crystal, 95% ethanol or 96% double dealdehyde ethanol (odorless ethanol) is used, and it is configured into an ethanol menthol solution at a ratio of 2:1 to ethanol. Different embodiments, about 10 to 20 parts, the minimum amount of the component can have an effect, and the number of parts is adjusted only due to different regulatory levels, resulting in the influence of the proportion of other components.

[0069] Thyme oil / essential oil, thyme (THYMUS VULGARIS) flower / leaf / stem extract, extracted by steam distillation; after extraction, it is a colorless, transparent to light yellow oily liquid with good liquid fluidity; because the regulatory authorities supervise thyme extract and thymol under the same conditions, the pharmacological effects of thyme extract are: thymol has a stronger bactericidal effect than phenol and low toxicity, has bactericidal and fungicidal effects on oral and pharyngeal mucosa, has antiseptic and local anesthetic effects on caries cavities, and is used for oral and pharyngeal disinfection, dermatophytosis, actinomycosis and otitis. It can promote tracheal cilia movement, is conducive to the secretion of tracheal mucus, is easy to expectorate, and has a bactericidal effect, so it can be used to treat tracheitis, whooping cough, etc. Regulatory level: food, health products (supplies), cosmetics, and medicines can add raw materials. Different embodiments, about 5 to 10 parts, the minimum amount of ingredients can have an effect, and the number of parts is adjusted only due to different regulatory levels, resulting in the influence of the proportion of other ingredients.

[0070] Vitamin E oil, Vitamin E / Tocopheryl acetate (TOCOPHERYL ACETATE), is used as a fat-soluble natural antioxidant, stabilizer, and thickener, so it uses purchased food or pharmaceutical grade raw materials. Management level: Food, health products (articles), cosmetics, and pharmaceutical additives. The proportion of each implementation plan is 5 to 10 parts, about 0.7% to 1% content.

[0071] Grapeseed oil / essential oil, hydrogenated grapeseed oil (HYDROGENATED GRAPESEED OIL), purchased raw materials, mainly cold pressed. This ingredient is used as a base oil (also known as base oil) to lubricate the skin. In addition to being used directly for massaging the skin, the base oil is also a base oil for diluting essential oils. Management level: food, health products (articles), cosmetics, and medicines can be added to raw materials. The proportion of each implementation plan is 50 to 100 parts, about 10 to 15% content.

[0072] Furthermore, a plant-extracted volatile oil having fatigue-relieving and analgesic effects comprises the following components in parts by weight:

[0073] 10 parts of pepper oil, 5 parts of pepper oil, 10 parts of camphor bark oil, 20 parts of camphor leaf oil, 20 parts of eucalyptus oil, 5 parts of peppermint oil, 15 parts of peppermint oil, 15 parts of ginger oil, 20 parts of clove bud oil, 5 parts of clove leaf oil, 15 parts of cinnamon oil, 10 parts of cassia oil, 5 parts of fennel oil, 15 parts of star anise oil, 20 parts of clove basil oil, 10 parts of wormwood oil, 20 parts of thyme oil, 10 parts of linalool thyme oil, 10 parts of patchouli oil, 10 parts of nutmeg oil, 10 parts of notopterygium oil, 5 parts of angelica oil, 5 parts of smilax oil, 10 parts of eucommia oil, 10 parts of dipsaci oil, 10 parts of sedge oil, 10 parts of windproof oil 1 part, 1 part of perilla leaf oil, 1 part of magnolia flower oil, 5 parts of calamus oil, 5 parts of ginkgo leaf oil, 10 parts of tangerine peel oil, 1 part of rose oil, 10 parts of rhodiola rosea oil, 5 parts of ginseng oil, 10 parts of atractylodes oil, 10 parts of atractylodes oil, 10 parts of magnolia oil, 10 parts of magnolia oil, 10 parts of chuanxiong oil, 5 parts of safflower oil, 2 parts of saffron oil, 5 parts of angelica oil, 5 parts of angelica oil, 5 parts of salvia oil, 5 parts of frankincense oil, 5 parts of myrrh oil, 5 parts of benzoin oil, 3 parts of storax oil, 5 parts of costus root oil, 5 parts of cyperus oil, 1 part of agarwood oil, 1 part of sandalwood oil, 5 parts of dalbergia oil, 20 parts of menthol, 40 parts of ethanol, 5 parts of vitamin E oil and 50 parts of grape seed oil.

[0074] Furthermore, it also includes flavoring agents.

[0075] Furthermore, the flavoring agent is linalool thyme oil, Roman chamomile oil, jasmine essential oil, clary sage oil, rose oil or lavender oil.

[0076] Linalool thyme oil / essential oil, thyme (THYMUS VULGARIS) flower / leaf oil, extracted by steam distillation; after extraction, it is a colorless, transparent to light yellow oily liquid with good liquid fluidity; this ingredient is a dual-purpose ingredient, used as a flavoring agent / aromatic agent, and enhances the taste of agarwood. The main component of agarwood extract is linalool, which is used for specific fragrances (models) and has a lower proportion. The proportion of each implementation plan is 1 to 5 parts.

[0077] Roman chamomile oil, white chamomile (ANTHEMIS NOBILIS) flower oil, is extracted by steam distillation; after extraction, it is a light blue to light yellow oily liquid with good liquid fluidity; this ingredient is mainly used as a flavoring agent / aromatic agent, and is used in specific fragrances (models) and the proportion is relatively low. The proportion of each implementation plan is 1 to 5 parts.

[0078] Jasmine oil / essential oil, jasmine (JASMINUM SAMBAC) flower oil, flowers of Oleaceae plants, the standard for Chinese medicine pieces is dried flowers, the volatile oil components are difficult to extract, this component is mainly used as a flavoring agent / aromatic agent, specific fragrance (model) use, the proportion is low, and the flower fragrance obviously masks other fragrances, it is not added as a basic formula, so purchased food or pharmaceutical grade raw materials are used. This raw material also has certain Chinese medicine effects, the effects are: regulating qi, relieving depression, harmonizing the middle, and eliminating filth. The main treatments of Chinese medicine are: used for chest discomfort, diarrhea and abdominal pain, dizziness and headache, red eyes, and sores. Regulatory level: Food, health products (supplies), cosmetics, and medicines can add raw materials. The proportion of each implementation plan is 1 to 5.

[0079] Sage oil / essential oil, sage (SALVIA JAPONICA) extract, extracted by steam distillation; after extraction, it is a light yellow oily liquid with good liquid fluidity; this ingredient is mainly used as a flavoring agent / aromatic agent, and is used in specific fragrances (models) and the proportion is relatively low. The proportion of each implementation plan is 1 to 5 parts.

[0080] Lavender oil / essential oil, sage (SALVIA JAPONICA) extract, extracted by steam distillation; after extraction, it is a light yellow oily liquid with good liquid fluidity; this ingredient is mainly used as a flavoring agent / aromatic agent, and the flower fragrance obviously masks other aromas and is not added as a basic formula, but is used for specific fragrances (models) and the proportion is relatively low. The proportion of each implementation plan is 1 to 5 parts.

[0081] The method for preparing the plant-extracted volatile oil having fatigue-relieving and analgesic effects comprises the following steps:

[0082] (1) respectively extracting angelica, angelica dahurica, Zanthoxylum bungeanum, saffron, atractylodes macrocephala, ligusticum chuanxiong, safflower, costusroot, cyperus rotundus, atractylodes lancea, magnolia officinalis, storax, agarwood, sandalwood, benzoin, angelica pubescens, dalbergia odorifera, myrrh, siler, and pepper using a supercritical CO2 extraction process to obtain corresponding essential oils;

[0083] (2) respectively extracting ginseng, patchouli, nutmeg, peppermint, mint, eucommia, ginger, perilla leaf, tangerine peel, clove leaf, clove bud, cinnamon, fennel, star anise, rhodiola rosea, salvia miltiorrhiza, ginkgo leaf, camphor bark, camphor leaf, frankincense, magnolia flower, eucalyptus leaf, calamus, rose, thyme, Roman chamomile, clove basil, lavender, wormwood, notopterygium wilfordii, smilax glabra, cassia twig, dipsacus, and sambucus by steam distillation to obtain corresponding essential oils;

[0084] (3) Vitamin E oil, menthol, ethanol, and grape seed oil as additives;

[0085] Dissolve the ethanol and menthol in a weight ratio of 2:1 to obtain an ethanol-menthol solution for later use;

[0086] Menthol is in crystalline form and can be directly and completely dissolved in vegetable oil. The purpose of preparing ethanol menthol solution in advance is to reduce the stirring time and minimize the volatilization of volatile oil.

[0087] (4) Mix and stir the essential oils with good liquid fluidity at a stirring speed of 10 rpm;

[0088] (5) Continue to add essential oil with poor liquid fluidity and mix and stir at a stirring speed of 15 to 25 rpm for 45 to 60 min;

[0089] (6) adjusting the speed to 10 rpm, adding rose oil, storax oil, agarwood oil, sandalwood oil, benzoin oil, saffron oil, frankincense oil and ethanol menthol solution, and stirring for 30 min;

[0090] (7) Allow to stand and then fill.

[0091] The filling is brown glass bottle with glass bead roll-on smear bottle, brown glass spray bottle, frosted transparent BFS disposable package for single-dose administration. Any of the above packaging can be used.

[0092] Furthermore, if a flavoring agent is required, it is added in step (6).

[0093] Further, the essential oil with good liquid fluidity is clove bud oil, clove leaf oil, pepper oil, atractylodes oil, chuanxiong oil, safflower oil, costus root oil, cyperus oil, ginseng oil, patchouli oil, nutmeg oil, peppermint oil, peppermint oil, ginger oil, perilla leaf oil, tangerine peel oil, cinnamon oil, fennel oil, star anise oil, salvia oil, ginkgo leaf oil, camphor bark oil, camphor leaf oil, magnolia flower oil, eucalyptus oil, calamus oil, clove basil oil, rhodiola rosea oil, thyme oil (thyme oil), agarwood thyme oil, wormwood oil, notopterygium oil, angelica dahurica oil, smilax glabra oil, dalbergia odorifera oil, eucommia oil, cassia twig oil, dipsaccharum officinale oil, sage oil, saposhnikovia oil, pepper oil, and grape seed oil.

[0094] Furthermore, the essential oils with poor liquid fluidity are angelica oil, angelica dahurica oil, atractylodes oil, magnolia bark oil, myrrh oil, and vitamin E oil.

[0095] Furthermore, the specific operation of the supercritical CO2 extraction process in step (1) is as follows:

[0096] (11) washing and crushing the raw materials, and respectively putting them into a supercritical extraction kettle;

[0097] (12) Cooling the storage tank and heating the extraction kettle and the analytical kettle at the same time, and pressurizing the extraction kettle and the analytical kettle when the temperature is heated to 60 to 160° C.;

[0098] (13) When the pressure reaches 5.0-30 MPa, the cyclic extraction begins;

[0099] (14) Maintaining a constant temperature and pressure of 60° C. and 5.0 MPa, the extraction was performed for 2 hours to extract the volatile oil and collect it in a packaging container for later use.

[0100] Furthermore, the specific operation of the steam distillation extraction process in step (2) is:

[0101] (21) washing and crushing the raw materials, placing them in distillation tanks respectively, and injecting clean water;

[0102] (22) heating and pressurizing with steam, boiling and evaporating at 700-1400 Pa and 100-120° C. to form oil-water mixture steam;

[0103] (23) obtaining a mixture of essential oil and water through a condenser;

[0104] (24) Separate the oil from the water to obtain the volatile oil, which is then sealed in an aluminum bottle and stored in a cool place for later use.

[0105] The application of the plant-extracted volatile oil with fatigue-relieving and analgesic effects in the preparation of anti-inflammatory, analgesic and fatigue-relieving drugs.

[0106] The invention discloses an application of the plant-extracted volatile oil with fatigue-relieving and analgesic effects in preparing a medicine for treating myalgic encephalomyelitis-chronic fatigue syndrome.

[0107] It can be seen from the above technical solution that, compared with the prior art, the beneficial effects achieved by the present invention are:

[0108] Theoretical basis of Chinese medicine prescription:

[0109] The prescription model of the present invention follows the theoretical basis of "monarch, minister, assistant and envoy" of traditional Chinese medicine prescription, and the Meta and CiteSpace analysis and collation of literature related to chronic fatigue syndrome, bone and joint, and fibromyalgia published from 2020 to June 30, 2023 (sorted by prescription and sorted by effective drugs twice in total), and summarizes the most effective qi-promoting-depression-relieving-blood circulation and analgesic prescription model and drug ratio. This is the first time that the present invention has used modern pharmaceutics-effective substance extraction technology-aromatic Chinese medicine effective substances-volatile oils to realize this prescription model, and through the formulation, some modern studies have eliminated (or replaced) substances that are considered to be slightly toxic. Compared with traditional decoctions and Chinese patent medicines, it has the characteristics of high drug loading, less impurities, and good transdermal absorption, which is of epoch-making significance.

[0110] Modern medicine and pharmacology basis:

[0111] This invention follows the drug treatment part (including compound drugs) and rehabilitation and quality of life improvement part of the "2021 NICE Guidelines: Diagnosis and Management of Myalgic Encephalomyelitis (or Encephalopathy) / Chronic Fatigue Syndrome (NG.206)". A compound prescription with analgesia, anti-inflammation, immunomodulation, aromatherapy & transdermal antidepressant is selected to comprehensively improve the quality of life and symptoms of chronic fatigue syndrome in patients.

[0112] The prescription part also selected those that are in line with the anti-inflammatory and analgesic effects clearly stated in the first part of the "Chinese Pharmacopoeia 2020 Edition": Clove basil oil-eugenol, star anise oil-trans-anethole, patchouli oil-patchouli alcohol, salvia miltiorrhiza extract-rosmarinic acid, cinnamon oil-cinnamaldehyde, eucalyptus oil-eucalyptol, peppermint oil-menthol, menthol (single ingredient), etc.; the above ingredients are not only effective ingredients, but also can be used as inspection standards, which meet international standards, and the standardized production and use safety level of products have been taken to a higher level.

[0113] On the premise that the active ingredients are effective, in order to solve the problem of transdermal absorption of preparations (products), after comparison of domestic and foreign literature, the dosages of 9 ingredients, including clove bud oil, peppermint oil, angelica oil, chuanxiong oil, pepper oil, eucalyptus leaf oil, atractylodes oil, cyperus oil, saposhnikovia divaricata, pepper oil (small dosage), calamus (weak), and notopterygium wilfordii (weak), were adjusted to solve the problem of transdermal absorption of the active ingredients. Natural volatile oils promote transdermal absorption, and one ingredient has two effects. Moreover, the above ingredients have undergone basic toxicology tests in major universities, and the ingredients are mild and effective, with sufficient evidence-based evidence.

[0114] Economic basis of drug treatment:

[0115] Since the present invention aims to solve a chronic symptom and the syndrome has a certain recurrence rate, the affected muscle groups and parts have a large medication area - the cost of a single treatment and the therapeutic effect will be a prominent contradiction. Therefore, after comparing with 2 Chinese medicine tinctures, 3 Chinese medicine patches, 2 chemical medicine creams and 1 chemical medicine patch with a large prescription ratio on the market, calculated according to the winning bid price of the medical institution, the first comparison shows that the treatment cost of the present invention is not advantageous. The main reason is that the cost of Chinese medicinal materials has increased significantly recently, resulting in a relatively high cost for extracting volatile oils. Therefore, after repeated experiments, adjustment of the prescription ratio, and increase of base oil (base oil), the cost of a single treatment is controlled within the range of the national low-priced medicine-Chinese patent medicine treatment cost, which is better than the current single treatment cost of all Chinese medicine external use, slightly higher than the treatment cost of a single chemical ingredient cream, reducing medical insurance and personal treatment expenses, greatly facilitating the majority of patients, and correspondingly improving treatment compliance, thereby achieving the purpose of a virtuous circle. BRIEF DESCRIPTION OF THE DRAWINGS

[0116] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the provided drawings without paying creative work.

[0117] Figure 1 The attached figure is a picture of the Nanjing Calvin KW-11A bipedal balance pain meter in Experiment 2 of the present invention;

[0118] Figure 2 This is the result of Experimental Mouse 1 in Experiment 2 of the present invention;

[0119] Figure 3 This is the result of Experimental Mouse 2 in Experiment 2 of the present invention;

[0120] Figure 4 This is the result of Experimental Mouse 3 in Experiment 2 of the present invention;

[0121] Figure 5 This is the result of Experimental Mouse 4 in Experiment 2 of the present invention;

[0122] Figure 6 This is the VE product analysis report of the present invention;

[0123] Figure 7 This is the product analysis report of jasmine essential oil of the present invention;

[0124] Figure 8 The menthol product analysis report of the present invention;

[0125] Fig. 9 This is the grape seed oil product analysis report of the present invention;

[0126] Fig.10 The invention discloses a KW-6D-S rat rotarod fatigue apparatus (with electrical stimulation). DETAILED DESCRIPTION

[0127] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0128] The drugs required for the present invention are conventional experimental drugs, which are purchased from commercial channels; the experimental methods not mentioned are conventional experimental methods and will not be described in detail here.

[0129] Example 1

[0130] 1) Take angelica, angelica dahurica, Zanthoxylum bungeanum, saffron, atractylodes, ligusticum chuanxiong, safflower, costusroot, cyperus rotundus, atractylodes, magnolia bark, storax, agarwood, sandalwood, benzoin, angelica pubescens, dalbergia odorifera, myrrh, siler, and pepper respectively, and extract essential oils by supercritical CO2 extraction process, and the component ratio and content are calculated according to the weight of the extract after extraction.

[0131] The supercritical CO2 extraction process is as follows: the plants are roughly cleaned and roughly crushed, and put into the supercritical extraction kettle respectively, the storage tank is cooled, and the extraction kettle and the decomposition kettle are heated at the same time. When the specified temperature (100°C) is reached, the CO2 cylinder is opened to deliver gas, and the high-pressure pump is turned on to pressurize the extraction kettle and the decomposition kettle. When the pressure reaches the set value (20MPa), the cyclic extraction is started. At this time, the flow rate is adjusted to maintain a constant temperature and pressure (60°C / 5.0MPa). The extraction time is 2 hours. The extracted material is collected in a packaging container for standby use.

[0132] 2) Take Eucommia ulmoides, ginseng, patchouli, nutmeg, mint leaves, whole mint, ginger, perilla leaves, tangerine peel, clove buds, clove leaves, cinnamon, fennel, star anise, rhodiola rosea, salvia miltiorrhiza, ginkgo leaves, camphor bark, camphor leaves, frankincense, magnolia flowers, eucalyptus leaves, calamus, rose, thyme, Roman chamomile, clove basil, lavender, wormwood, notopterygium wilfordii, spatholobi, cassia twig, dipsacus, and spondias serrata by steam distillation extraction, and the component ratio and content are calculated by the weight of the extract after extraction.

[0133] The steam distillation extraction process is as follows: the plants are roughly cleaned and roughly crushed, placed in a distillation tank respectively, and clean water is injected. The plants are heated and pressurized (1000Pa) by steam, and then boiled (110°C) to form an oil-water mixture. When the temperature continues to rise, the oil-water mixture evaporates rapidly, and the steam of the mixture is generated. A mixture of essential oil and water is obtained through a condenser. The essential oil floats on the water, and the oil and water are separated. The essential oil on top is taken, sealed in an aluminum bottle and stored in a cool place for later use.

[0134] 3) Vitamin E oil (tocopherol acetate) as a stabilizer and additive is a purchased raw material. The product analysis report is as follows: Figure 6 As shown;

[0135] Grape seed oil (cold pressing process) is used as base oil-base oil, which plays a lubricating and diluting role and is a purchased raw material. The product analysis report is as follows Fig. 9 As shown;

[0136] Menthol and 95% ethanol as skin feel regulators (cooling) are purchased raw materials. The product analysis report of menthol is as follows: Figure 8As shown; and prepare an ethanol menthol solution, dissolve menthol in a ratio of 2:1 (mass ratio) with ethanol for later use, that is, 40 parts of ethanol dissolve 20 parts of menthol. Menthol is in a crystalline form and can be directly and completely dissolved in vegetable oil. The purpose of preparing the ethanol menthol solution in advance is to reduce the stirring time and minimize the volatilization of volatile oil.

[0137] 4) First, the extracted volatile oils are distinguished according to their fluidity, and the raw materials with better fluidity are first added into a closed upper and lower conical stirring tank at room temperature, and an anchor or frame stirring head is used to avoid stirring heat and reduce the stirring contact surface. The raw materials that can be added for the first time are: 10 parts of Atractylodes macrocephala oil, 10 parts of Chuanxiong oil, 5 parts of safflower oil, 5 parts of costus root oil, 5 parts of Cyperus rotundus oil, 10 parts of Zanthoxylum oil, 5 parts of pepper oil, 10 parts of Rhodiola rosea oil, 5 parts of ginseng oil, 10 parts of camphor bark oil, 20 parts of camphor leaf oil, 20 parts of eucalyptus oil, 10 parts of patchouli oil, 10 parts of nutmeg oil, 5 parts of salvia miltiorrhiza oil, 5 parts of peppermint oil, 5 parts of thin 15 parts of lotus oil, 15 parts of ginger oil, 1 part of perilla leaf oil, 1 part of magnolia flower oil, 5 parts of calamus oil, 5 parts of ginkgo leaf oil, 10 parts of tangerine peel oil, 20 parts of clove bud oil, 5 parts of clove leaf oil, 15 parts of cinnamon oil, 10 parts of cassia oil, 5 parts of fennel oil, 15 parts of star anise oil, 20 parts of clove basil oil, 20 parts of thyme oil (thyme oil), 10 parts of linalool thyme oil, 10 parts of wormwood oil, 10 parts of notopterygium oil, 5 parts of angelica root oil, 5 parts of smilax oil, 10 parts of eucommia oil, 10 parts of dipsaci oil, 10 parts of sedge oil, 10 parts of saposhnikovia oil, and 50 parts of grape seed oil;

[0138] 7) Turn on the frequency converter of the closed upper and lower conical normal temperature stirring tank, set a lower speed of 10 rpm to ensure that the previously added oil-loaded liquid is in a stirring state, and then add liquid with poor fluidity from the upper feed port to extract volatile oil to avoid sticking loss of raw materials; the raw materials added in the second step are: 5 parts of Atractylodes oil, 10 parts of Magnolia oil, 5 parts of Angelica oil, 5 parts of Angelica dahurica oil, 5 parts of Myrrh oil, and 5 parts of Vitamin E oil; after adding viscous and oily raw materials, set the stirring speed to 20 rpm and the stirring time to 50 minutes. Since the raw materials are all fat-soluble liquids with good solubility and no precipitation or impurities, ensure that the equipment does not generate heat during the stirring process, resulting in heat volatilization, reduced effect or increased production loss.

[0139] 8) After the second step of stirring is completed, the frequency converter is turned on and a relatively low speed of 10 rpm is set. Finally, 2 parts of saffron oil, 3 parts of storax oil, 1 part of agarwood oil, 1 part of sandalwood oil, 5 parts of benzoin oil, 5 parts of frankincense oil, 1 part of rose oil, and 60 parts of ethanol menthol solution (20 parts of menthol and 40 parts of 95% ethanol) prepared in advance are added from the upper feed port. The more expensive medicinal materials, since the raw materials added at the end are all raw materials with good liquid fluidity and are fat-soluble, there is no need to adjust the speed. The stirring time is 30 minutes. After the stirring is completed, it is allowed to stand for 60 minutes and sampled. The finished product should be a bright brown or dark brown liquid fluidity and should not be stratified after standing.

[0140] 9) Transfer from the closed upper and lower conical stirring tank at room temperature through a pipeline to the automated filling equipment or the blow-fill-seal integrated machine (Blow Fill Seal / BFS, Technical Guideline of Infusion Production of Blow Fill Seal-BFS). It is also necessary to stand for 30 minutes before filling, and the filling speed and heat sealing speed should be controlled to be reduced. Since the fat-soluble liquid itself has the physical property of pipe adhesion, there may be a problem of inaccurate dosage due to the loss of production pipeline equipment. The designed filling should be 105% to 115% of the labeled filling amount (adjusted by the number and length of the filling heads), and the loss of the efficacy of the extracted essential oils due to the generation of bubbles or the production of heat should be avoided as much as possible.

[0141] 10) The filling is brown glass bottle with glass bead roll-on applicator, or brown glass spray bottle, or frosted transparent BFS disposable package for single-dose packaging.

[0142] The present invention is in the form of a Chinese patent medicine for external use, which has the characteristics of high skin safety, high active ingredient loading under the same volume, easy to carry, and high patient compliance. It can relieve bone and joint pain caused by inflammation in combination with specific acupuncture points of traditional Chinese medicine and massage and application rollers, and is used for muscle tissue pain caused by frozen shoulder, tenosynovitis, cervical spondylosis, osteoarthritis, lumbar muscle strain, sprain and contusion, etc.

[0143] Example 2

[0144] 1) Take angelica, angelica dahurica, prickly ash, saffron, atractylodes, chuanxiong, safflower, costusroot, cyperus rotundus, atractylodes, magnolia bark, storax, agarwood, sandalwood, benzoin, angelica pubescens, dalbergia odorifera, myrrh, siler, and pepper, and use a supercritical CO2 extraction process, and the component ratio and content are calculated according to the weight of the extract after extraction.

[0145] 2) The extraction kettle and the desorption kettle reach the specified temperature (60° C.), and the pressure reaches the set value (5.0 MPa); the rest of the extraction process is the same as in Example 1.

[0146] 3) Take Eucommia ulmoides, ginseng, patchouli, nutmeg, mint leaves, whole mint, ginger, perilla leaves, tangerine peel, clove leaves, clove buds, cinnamon, fennel, star anise, rhodiola rosea, salvia miltiorrhiza, ginkgo leaves, camphor bark, camphor leaves, frankincense, magnolia flowers, eucalyptus leaves, calamus, rose, thyme, Roman chamomile, clove basil, lavender, wormwood, notopterygium wilfordii, spatholobi, cassia twig, dipsacus, and sambucus by steam distillation extraction, and the component ratio and content are calculated by the weight of the extract after extraction.

[0147] 4) The mixture is heated and pressurized by steam (700 Pa), and then boiled (100° C.) to form an oil-water mixture. The rest of the extraction process is the same as in Example 1.

[0148] 5) Vitamin E (tocopherol acetate) is a purchased raw material as a stabilizer and additive; grape seed oil (cold pressing process) is a base oil-carrier oil that plays a lubricating and diluting role and is a purchased raw material; menthol and 95% ethanol are purchased raw materials as skin feel regulators (cooling feeling); and an ethanol menthol solution is prepared, and the menthol is dissolved in a ratio of 2:1 (mass ratio) with ethanol for later use, that is, 40 parts of ethanol dissolve 20 parts of menthol. Menthol is in a crystalline form and can be directly and completely dissolved in vegetable oil. The purpose of preparing the ethanol menthol solution in advance is to reduce the stirring time and minimize the volatilization of volatile oils.

[0149] 6) First, the extracted volatile oils are distinguished according to their fluidity, and the raw materials with better fluidity are first added into a closed upper and lower conical stirring tank at room temperature, and an anchor or frame stirring head is used to avoid stirring heat and reduce the stirring contact surface. The raw materials that can be added for the first time are: 20 parts of Atractylodes macrocephala oil-(food), 10 parts of Chuanxiong oil-(health care), 5 parts of safflower oil-(health care), 5 parts of costus root oil-(health care), 5 parts of cyperus rotundus oil-(health care), 15 parts of Zanthoxylum oil-(food) , 5 parts of pepper oil - (food), 10 parts of rhodiola rosea oil - (health care), 5 parts of ginseng oil - (food), 15 parts of camphor bark oil, 40 parts of camphor leaf oil, 40 parts of eucalyptus oil - (health care), 20 parts of patchouli oil - (food), 20 parts of nutmeg oil - (food), 10 parts of salvia oil - (health care), 10 parts of peppermint oil, 30 parts of peppermint oil - (food), 30 parts of ginger oil - (food), 1 part of perilla leaf oil - (food), 1 part of magnolia flower oil , 5 parts of calamus oil, 10 parts of ginkgo leaf oil - (health care), 20 parts of tangerine peel oil - (food), 40 parts of clove bud oil - (food), 10 parts of clove leaf oil - (food), 30 parts of cinnamon oil - (food), 10 parts of cassia oil, 10 parts of fennel oil - (food), 30 parts of star anise oil - (food), 20 parts of clove basil oil, 20 parts of thyme oil (thyme oil), 10 parts of linalool thyme oil, 20 parts of wormwood oil - (food), Qiang 10 parts of angelica oil, 1 part of angelica dahurica oil, 5 parts of smilax china oil, 5 parts of dalbergia odorifera oil, 10 parts of eucommia oil - (health care), 10 parts of knotweed oil, 10 parts of sedge oil, 20 parts of saposhnikovia oil - (food), 100 parts of grape seed oil - (food); Remarks: The remarks in brackets about food and health care are not differences in raw material levels (grades), but are raw materials that can be added to foods and health products. The focus is on clarifying the safety of the formula, that is, the proportion of ingredients of similar origin to medicine and food in the total ingredients. The same applies hereinafter.

[0150] 7) Turn on the frequency converter of the closed upper and lower conical normal temperature stirring tank, set a lower speed of 10 rpm to ensure that the previously added oil-containing liquid is in a stirring state, and then add liquid with poor fluidity from the upper feed port to extract volatile oil to avoid sticking loss of raw materials; the raw materials added in the second step are: 5 parts of Atractylodes oil, 10 parts of Magnolia oil, 10 parts of Angelica oil-(food), 5 parts of Angelica dahurica oil-(food), 5 parts of Myrrh oil, 5 parts of Vitamin E oil-(food); after adding viscous and oily raw materials, set the stirring speed to 15-25 rpm, and the stirring time to 45-60 minutes. Since the raw materials are all fat-soluble liquids with good solubility and no precipitation or impurities; ensure that the equipment does not generate heat during the stirring process, resulting in heat volatilization, reduced effect or increased production loss.

[0151] 8) After the second step of stirring is completed, turn on the frequency converter, set a lower speed of 10 rpm, and finally add 0.5 parts of saffron oil-(food), 2 parts of storax oil, 0.5 parts of agarwood oil, 1 part of sandalwood oil, 1 part of benzoin oil, 1 part of frankincense oil, 1 part of rose oil-(food), 60 parts of ethanol menthol solution (20 parts of menthol-(food), 40 parts of 95% ethanol) prepared in advance, or jasmine oil (product analysis report as shown in Figure 1) from the upper feed port. Figure 7 As shown in the figure, 1-5 parts of rose oil, 1-5 parts of linalool thyme oil, 1-5 parts of Roman chamomile oil, 1-5 parts of lavender oil, 1-5 parts of sage oil and other flavoring agents are used to determine the fragrance. Since the raw materials added last are all raw materials with good liquid fluidity and are all fat-soluble, there is no need to adjust the speed. The stirring time is 30 minutes. After the stirring is completed, let it stand for 60 minutes and take samples. The finished product should be a bright brown or dark brown liquid and should not be separated after standing.

[0152] 9) The filling method is the same as in Example 1.

[0153] 10) The product is packaged in brown glass bottles with glass bead roll-on applicators (including large-capacity roll-on applicators), or brown glass spray bottles, or frosted transparent BFS disposable packaging for single-dose administration.

[0154] 11) The present invention has a total of 7 preset flavors, including original drug flavor (without adding flavoring agent), rose flavor, jasmine flavor, agarwood thyme flavor, chamomile flavor, clary perilla flavor, and lavender flavor. Other flavors are adjusted and added according to the safety publicity of administrative approval of raw materials.

[0155] 12) The present invention is in the form of an external health product (health care product). The prescription ratio is adjusted according to the food-grade raw material publicity in the 2022 administrative approval publicity. The addible ingredients of medicinal and edible origin account for more than 73.6%, skin feel regulators account for 16.8%, and the total safe ingredients are more than 90%. It is implemented with safety as the focus and is aimed at a wide range of consumers. It can relieve bone and joint and muscle pain caused by inflammation in combination with specific acupuncture points and massage rollers in traditional Chinese medicine, or application during physical therapy, and can be used to relieve muscle pain caused by mechanical fatigue or strain such as driving and studying.

[0156] Example 3

[0157] 1) Take angelica, angelica dahurica, prickly ash, saffron, atractylodes, chuanxiong, safflower, costusroot, cyperus rotundus, atractylodes, magnolia bark, storax, agarwood, sandalwood, benzoin, angelica pubescens, dalbergia odorifera, myrrh, siler, and pepper, and use a supercritical CO2 extraction process, and the component ratio and content are calculated according to the weight of the extract after extraction.

[0158] 2) The extraction kettle and the desorption kettle reach the specified temperature (160° C.) and the pressure reaches the set value (30 MPa); the rest of the extraction process is the same as in Example 1.

[0159] 3) Take Eucommia ulmoides, ginseng, patchouli, nutmeg, mint leaves, whole mint, ginger, perilla leaves, tangerine peel, clove leaves, clove buds, cinnamon, fennel, star anise, rhodiola rosea, salvia miltiorrhiza, ginkgo leaves, camphor bark, camphor leaves, frankincense, magnolia flowers, eucalyptus leaves, calamus, rose, thyme, Roman chamomile, clove basil, lavender, wormwood, notopterygium wilfordii, spatholobi, cassia twig, dipsacus, and sambucus by steam distillation extraction, and the component ratio and content are calculated by the weight of the extract after extraction.

[0160] 4) The oil-water mixture is formed by heating and pressurizing with steam (1400 Pa) and boiling (120° C.) and vaporizing. The rest of the extraction process is the same as in Example 1.

[0161] 5) Vitamin E (tocopherol acetate) is a purchased raw material as a stabilizer and additive; grape seed oil (cold pressing process) is a base oil-carrier oil that plays a lubricating and diluting role and is a purchased raw material; menthol and 95% ethanol are purchased raw materials as skin feel regulators (cooling feeling); and an ethanol menthol solution is prepared, and the menthol is dissolved in a ratio of 2:1 (mass ratio) with ethanol for later use, that is, 40 parts of ethanol dissolve 20 parts of menthol. Menthol is in a crystalline form and can be directly and completely dissolved in vegetable oil. The purpose of preparing the ethanol menthol solution in advance is to reduce the stirring time and minimize the volatilization of volatile oils.

[0162] 6) First, the extracted volatile oils are distinguished according to their fluidity, and the raw materials with better fluidity are first added into a closed upper and lower conical stirring tank at room temperature, and an anchor or frame stirring head is used to avoid stirring heat and reduce the stirring contact surface. The raw materials that can be added for the first time are: 20 parts of Atractylodes macrocephala oil, 20 parts of Chuanxiong oil, 1 part of safflower oil, 5 parts of costus root oil, 5 parts of cyperus oil, 15 parts of Zanthoxylum oil, 5 parts of pepper oil, 20 parts of rhodiola rosea oil, 1 part of ginseng oil, 40 parts of camphor bark oil, 40 parts of camphor leaf oil, 40 parts of eucalyptus oil, 20 parts of patchouli oil, 20 parts of nutmeg oil, 5 parts of salvia miltiorrhiza oil, 10 parts of peppermint oil, 10 parts of thyme ... 30 parts of lotus oil, 30 parts of ginger oil, 1 part of perilla leaf oil, 1 part of magnolia flower oil, 1 part of calamus oil, 10 parts of ginkgo leaf oil, 20 parts of tangerine peel oil, 40 parts of clove bud oil, 15 parts of clove leaf oil, 15 parts of cinnamon oil, 10 parts of cassia oil, 10 parts of fennel oil, 30 parts of star anise oil, 20 parts of clove basil oil, 50 parts of thyme oil (thyme oil), 20 parts of linalool thyme oil, 20 parts of wormwood oil, 10 parts of notopterygium oil, 1 part of angelica dahurica oil, 3 parts of lycopodiella oil, 1 part of dalbergia odorifera oil, 20 parts of eucommia oil, 20 parts of dipsaci oil, 15 parts of sedge oil, 20 parts of saposhnikovia oil, and 100 parts of grape seed oil.

[0163] 7) Turn on the frequency converter of the closed upper and lower conical normal temperature stirring tank, set a lower speed of 10 rpm to ensure that the previously added oil-containing liquid is in a stirring state, and then add liquid with poor fluidity from the upper feed port to extract volatile oil to avoid sticking loss of raw materials; the raw materials added in the second step are: 20 parts of Atractylodes oil, 20 parts of Magnolia oil, 10 parts of Angelica oil, 1 part of Angelica dahurica oil, 5 parts of Myrrh oil, and 10 parts of Vitamin E oil; after adding viscous and oily raw materials, set the stirring speed to 15-25 rpm, and the stirring time to 45-60 minutes. Since the raw materials are all fat-soluble liquids with good solubility and no precipitation or impurities; ensure that the equipment does not generate heat during the stirring process, resulting in heat volatilization, reduced effect or increased production loss.

[0164] 8) After the second step of stirring is completed, the frequency conversion regulator is turned on, and a relatively low speed is set at 10 rpm. From the upper feed port, 0.5 part of saffron oil, 2 parts of storax oil, 0.5 part of agarwood oil, 2 parts of sandalwood oil, 2 parts of benzoin oil, 2 parts of frankincense oil, 1 part of rose oil, 60 parts of ethanol menthol solutions (20 parts of menthol, 40 parts of 95% ethanol) configured in advance are added at the end, or 1 to 5 parts of jasmine oil, 1 to 5 parts of rose oil, 1 to 5 parts of linalool thyme oil, 1 to 5 parts of Roman chamomile oil, 1 to 5 parts of lavender oil, 1 to 5 parts of clary sage oil and other flavoring agents are added to determine the fragrance type. Since the raw materials added at the end all belong to raw materials with good liquid fluidity, they are all fat-soluble, and there is no need to adjust the speed. The stirring time is 30 minutes. After the stirring is completed, stand for 60 minutes, sample, and the finished product should be a bright brown or dark brown fluid liquid, and stratification is not allowed after standing.

[0165] 9) The filling method is the same as in Example 1.

[0166] 10) The product is packaged in brown glass bottles with glass bead roll-on applicators (including large-capacity roll-on applicators), or brown glass spray bottles, or frosted transparent BFS disposable packaging for single-dose administration.

[0167] The present invention does not have a preset fragrance. Fragrances can be added according to the raw material reporting approved by administrative examination and approval, and all finished product fragrances are available. The body sensation regulator or cooling agent of the present invention can be adjusted in multiples to appear in a cooling enhancement form.

[0168] The present invention is in the form of aromatherapy cosmetics (aromatherapy daily chemical products) or massage plant essential oils. According to the 2022 administrative approval announcement, the raw materials can be added to adjust the prescription ratio; combined with specific acupuncture points and massage application rollers in traditional Chinese medicine, or applied during physical therapy, it can relieve bone and joint and muscle pain caused by inflammation, and is used to relieve muscle pain caused by mechanical fatigue or strain such as driving and studying.

[0169] Animal experiments were conducted using the product prepared in Example 1 of the present invention:

[0170] Experiment 1

[0171] Safety test:

[0172] 1) Multiple skin irritation tests, two groups of test rabbits, four rabbits in each group, the test doses were 3 times and 5 times the recommended daily dose respectively;

[0173] 2) Experimental preparation: The standard rabbits were purchased and raised for three days. The back muscles and the right hind leg joints were selected and the skin was prepared (shaved) as the experimental area. Considering the safety of the subsequent healthy volunteer subjects, the left-right control method was not used. Instead, the sample size was expanded and two groups were selected for the experiment;

[0174] 3) For routine use (i.e. recommended daily dosage), the drug should be administered 3 times a day, i.e. once every 6 hours, and a cotton swab should be used to apply the drug evenly; a 3-fold dose means the drug should be administered once every 2 hours, six times a day; a 5-fold dose means the drug should be administered once every 1 hour, 12 times a day; before administering the drug again, use a cotton swab dipped in pure water to clean the prepared skin area.

[0175] The scoring criteria were formulated in accordance with the "Guidelines for Skin Irritation Tests" of the State Food and Drug Administration, as shown in Table 1.

[0176] Table 1 Skin irritation response scoring criteria

[0177]

[0178] The test results are shown in Table 2.

[0179] Table 2 Safety test results

[0180]

[0181] The results of safety tests showed that in multiple skin irritation tests, the safety of the conventional 3-times dose test was 92.5%, the safety of the conventional 5-times dose test was 90%, and the average safety of the two multiple skin irritation tests was 91.25%. It can be relatively proved that this product has good safety and is almost non-irritating to non-sensitive skin; some mild erythema (barely visible) appeared all at the right hind leg joint test site, and there was no irritation-inflammatory reaction in the rich muscle areas of the back, and the formula ingredients are safe.

[0182] Experiment 2

[0183] Potassium ion / Freund's complete adjuvant-subcutaneous pain induction in rats

[0184] 1) Modeling materials [animals]: 4 rats (250-300 g); [drugs]: (1) saturated potassium chloride solution - to produce pain; (2) complete Freund's adjuvant (CFA) - to produce inflammatory response; [instrument]: Nanjing Calvin KW-11A bipedal balance analgesia instrument (such as Figure 1 =As shown); [Drugs / reagents]: Potassium chloride injection 10ml: 1g Tianjin Jinyao Pharmaceutical Co., Ltd.; Freund's complete adjuvant (Freund's Adjuvant, Complete) Product number: F5881 Brand: Sigma Merck KGaA 64271 Darmstadt Germany

[0185] 2) Modeling method: Prepare the skin (shave the hair) at the muscle-rich part of the right hind leg joint of the rat, and use potassium chloride injection or fully emulsified CFA to inject subcutaneously at the plantar part of the rat's hind limb, injecting 0.1 ml on one side at a time. After potassium chloride injection or CFA injection, observe the rat's reaction, joint swelling, and histopathological changes.

[0186] 3) Modeling principle Subcutaneous penetration of potassium ions causes pain reactions in animals. Immunizing animals with complete Freund's adjuvant (CFA) can stimulate the proliferation of immune cells, abnormally increase the immune function of the animal body, produce autoimmune reactions against the synovial membrane of the joints, and deposit immune complexes in the joint cavity, producing changes similar to rheumatoid arthritis.

[0187] 4) Changes after modeling: After injection, animals may experience pain reactions, such as motor reactions at the injection site (shaking legs), neighing, and strong tail shaking, accompanied by continuous neighing and whole-body struggles.

[0188] 5) Experimental design and purpose: Since most of the current standard animal test models for pain are designed for systemic administration scenarios such as oral or injection, the "Rat Pain Model Induced by Subcutaneous Potassium Ion Penetration" and "Adjuvant Arthritis Model" were combined, and the model was designed in reference to the "Technical Guidelines for Clinical Trials of Locally Administered Locally Effective Drugs" of the Center for Drug Evaluation (CDE) of the State Food and Drug Administration; the purpose is to verify the local analgesic effect of local short-term pain (potassium ions) and to verify the local analgesic effect of mild and moderate pain caused by local inflammation (Freund's complete adjuvant). The experimental comparison is a left-right control test of the same experimental rat. The test equipment is the Nanjing Calvin KW-11A bipedal balance analgesia instrument. There will be a difference in the strength to support body weight between the inflamed painful foot and the normal foot. The greater the difference, the more painful the inflamed foot is. The use of analgesic drugs can reduce the difference, so as to identify the efficacy of anti-inflammatory and analgesic drugs. The test accuracy can reach 0.01g, and the measurement range of 250-300g rats is 1g; the conversion tension curve range is: -400 to 800, and the actual single-foot curve is: 0-200, in g.

[0189] 6) Test standard: Use a balance-covered acrylic box to weigh each experimental mouse in detail and record the weight; put it into the Calvin KW-11A bipedal balance analgesia instrument, and every 5 minutes is an observation point (the equipment is in video monitoring mode-average value every minute, take a value every 5 minutes, a total of 7 marking points); take the unprepared left leg as the standard value, observe 0-30 minutes for potassium-induced pain on the first day, and observe 0-30 minutes for adjuvant-induced pain on the second day, which are used as pathogenic reference values ​​and recorded respectively; 5 minutes after the injection of potassium on the third day, use a cotton swab to smear the muscle-rich area (the product prepared in Example 1) and observe and record; 5 minutes after the injection of adjuvant on the fourth day, use a cotton swab to smear the muscle-rich area and observe and record.

[0190] 7) The test results are as follows:

[0191] The results are as follows Figures 2 to 5 shown.

[0192] The results of four control experiments show that, from the comparison of the experimental curves, it can be clearly concluded that the test has a high reproducibility, and the average total effective rate of the four experiments can reach 92%; and all of them can take effect 5 to 15 minutes after administration, proving that the present invention has good transdermal absorbability; it is effective for short-term pain caused by potassium agents, and for chronic mild and moderate pain caused by adjuvant inflammation. In comparison, the present invention is more effective for mild and moderate chronic pain caused by inflammatory mediators, which is consistent with the design of the ingredient formula ratio and the functional indications of the Chinese medicine formula model, and does not cover up the pain sensation through skin feel regulation to achieve the effect of relieving pain.

[0193] Experiment 3

[0194] Experiment on the rat model of chronic fatigue and motor limitation induced by inflammation by subcutaneous injection of Freund's complete adjuvant

[0195] 1) Modeling materials [Animals]: 3 rats (250-300 g); [Reagents]: complete Freund's adjuvant (CFA) - to produce inflammatory response; [Instruments]: KW-6D-S rat rotarod fatigue apparatus (with electrical stimulation); [Reagents]: complete Freund's Adjuvant (Complete) Product number: F5881 Brand: Sigma Merck KGaA 64271 Darmstadt, Germany.

[0196] 2) Modeling method: Prepare the skin (shave the hair) of the muscle-rich part of the upper part of the hind limb joint of the rat, and use the fully emulsified and mixed CFA to subcutaneously inject 0.1 ml into each side once. After CFA injection, observe the reaction, joint swelling, and histopathological changes of the rat.

[0197] 3) Modeling principle: Immunizing animals with complete Freund's adjuvant (CFA) can stimulate the proliferation of immune cells, abnormally increase the immune function of the animal body, produce an autoimmune response against the synovial membrane of the joint, and deposit immune complexes in the joint cavity, producing changes similar to the inflammatory response caused by lactic acid accumulation.

[0198] 4) Changes after modeling Injection is accompanied by inflammatory reaction, local tissue congestion, redness, swelling, pain, limited or reduced motor function, and electrical stimulation is also reduced due to the slow elimination of tissue inflammatory reaction.

[0199] 5) Experimental design and purpose: Since the present invention is a local drug administration, the standard "Acute Exercise Fatigue Animal Model" involves a swimming test-water dilution interference test result, and the "Chronic Exercise Fatigue Animal Model" involves an inflammatory response of lactic acid accumulation in multiple parts of the body that interferes with the test result. Therefore, a model expression of "Local Inflammatory Response Leading to Limited Motor Function" similar to Experiment 1 was established, that is, the fatigue-lactic acid accumulation inflammatory response caused by mechanical exercise of experimental animals was compared through local administration, such as reducing the inflammatory response to mechanical exercise.

[0200] 6) Test standard: Three test rats were placed in a KW-6D-S rat rotarod fatigue apparatus (with electrical stimulation) at the same time, and the number of movement circles was observed for 30 minutes under healthy conditions. The fall penalty time (electrical stimulation) was 15 seconds. The number of circles for both forward and reverse movements were recorded three times in parallel, and the average value was taken as the standard for healthy rats; after more than 2 hours of sufficient rest, Freund's complete adjuvant was injected to prepare a fatigue model of inflammatory response, and the number of movement circles was observed for 30 minutes. The fall penalty time (electrical stimulation) was 15 seconds. The number of circles were recorded three times in parallel, and the average value was taken as the standard for fatigue rats; on the second day, after the local inflammatory response of Freund's complete adjuvant was completely eliminated (to avoid inflammation accumulation), the fatigue inflammatory response model rats were re-made, and the muscles and joint tissues related to the inflammatory response were smeared to observe and record the improvement of the rats' movement.

[0201] 7) The test results are as follows:

[0202] Table 3 Results of rat chronic fatigue limited exercise model test

[0203] project Laboratory rat 1 Lab Mouse 2 Lab rat 3 Healthy laps (30min) 91 98 101 Inflammation circles (30min) 33 37 42 Treatment circle number (30min) 72 77 89 Improvement rate - inflammation 118.2％ 108.1％ 111.9％ Recovery Rate - Health 79.1％ 78.6％ 88.1％

[0204] The experimental results showed that the three rats with inflammation-induced fatigue model all showed significant improvement, and the problem of interference in animal experiments was solved by avoiding the rats' passive exercise factors through the use of electrical stimulation equipment. This objectively shows that local administration can significantly relieve the fatigue caused by inflammation and restore the body's motor function.

[0205] Experiment 4

[0206] Healthy volunteer population trial

[0207] It has been proven safe and effective through laboratory tests. The product avoids laboratory participants, publicizes the safety results of multiple skin irritation tests, and does not publicize other test results. In the meantime, the product is internally searched for employees or family members with a history of chronic muscle pain such as non-organic (bone) lesions - strain type, degenerative type, etc.

[0208] Fill 10ml frosted glass bottles - equipped with glass rollers - and distribute them to healthy volunteers. Tell them to use it when they have muscle soreness symptoms or when fatigue is unbearable. Apply it to muscle-dense areas where discomfort occurs. When an attack occurs, apply 3 to 4 times a day. The total observation time is 3 days. If it is ineffective after 3 days, take medication on your own or seek medical treatment, and the results will be summarized in the office's internal statistical records.

[0209] Evaluation criteria:

[0210] 1) Take the medicine 2 to 3 times a day continuously, and the symptoms will disappear within 3 days without recurrence, which is obvious.

[0211] 2) Take the medicine 3 to 4 times a day continuously. The symptoms disappear after taking the medicine, and the pain symptoms in the uncomfortable part do not recur within 4 hours after stopping the medicine. It is effective;

[0212] 3) The drug is used 3 to 4 times a day, and the symptoms disappear or do not disappear after taking the drug. The pain symptoms in the uncomfortable part recur within a short time after stopping the drug (the drug effect does not last for 4 hours), which means it is ineffective;

[0213] The experimental results are shown in Table 4.

[0214] Table 4 Test results of healthy volunteers

[0215]

[0216]

[0217] After testing on ten healthy volunteers, 90% of the people reported that the product was effective; only one healthy volunteer reported that there was a certain effect after applying it three times, and the symptoms were alleviated, but the effect was not obvious. However, the volunteer was a patient with a history of diagnosed lumbar disc herniation and compressive pain. Similar volunteer 3 was a female and had similar compressive pain symptoms, but reported that it was effective; it may be related to the compression site or volunteer 6's elderly bone degeneration, hyperplasia, etc. - bone organic lesions; all tests reported that it has a good effect on muscle pain caused by non-bone organic lesions, and has a good effect on mechanical fatigue - lactic acid accumulation - soreness, and local inflammatory response.

[0218] The various embodiments in this specification are described in a progressive manner, and each embodiment focuses on the differences from other embodiments. The same or similar parts between the various embodiments can be referenced to each other.

[0219] The above description of the disclosed embodiments enables one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. An external preparation of plant-extracted volatile oil with fatigue-relieving and analgesic effects, characterized in that: It is composed of the following components by mass: 10 parts of pepper oil, 5 parts of pepper oil, 10 parts of camphor bark oil, 20 parts of camphor leaf oil, 20 parts of eucalyptus oil, 5 parts of peppermint oil, 15 parts of peppermint oil, 15 parts of ginger oil, 20 parts of clove bud oil, 5 parts of clove leaf oil, 15 parts of cinnamon oil, 10 parts of cassia oil, 5 parts of fennel oil, 15 parts of star anise oil, 20 parts of clove basil oil, 10 parts of wormwood oil, 20 parts of thyme oil, 10 parts of linalool thyme oil, 10 parts of patchouli oil, 10 parts of nutmeg oil, 10 parts of notopterygium oil, 5 parts of angelica oil, 5 parts of smilax oil, 10 parts of eucommia oil, 10 parts of dipsaci oil, 10 parts of sedge oil, 10 parts of windproof oil 1 part, 1 part of perilla leaf oil, 1 part of magnolia flower oil, 5 parts of calamus oil, 5 parts of ginkgo leaf oil, 10 parts of tangerine peel oil, 1 part of rose oil, 10 parts of rhodiola rosea oil, 5 parts of ginseng oil, 10 parts of atractylodes oil, 10 parts of atractylodes oil, 10 parts of magnolia oil, 10 parts of magnolia oil, 10 parts of chuanxiong oil, 5 parts of safflower oil, 2 parts of saffron oil, 5 parts of angelica oil, 5 parts of angelica oil, 5 parts of salvia oil, 5 parts of frankincense oil, 5 parts of myrrh oil, 5 parts of benzoin oil, 3 parts of storax oil, 5 parts of costus root oil, 5 parts of cyperus oil, 1 part of agarwood oil, 1 part of sandalwood oil, 5 parts of dalbergia odorifera oil, 20 parts of menthol, 40 parts of ethanol, 5 parts of vitamin E oil and 50 parts of grape seed oil; The preparation method comprises the following steps: (1) Extracting angelica, angelica dahurica, Zanthoxylum bungeanum, saffron, atractylodes macrocephala, ligusticum chuanxiong, safflower, costusroot, cyperus rotundus, atractylodes lancea, magnolia officinalis, storax, agarwood, sandalwood, benzoin, angelica pubescens, dalbergia odorifera, myrrh, saposhnikovia divaricata, and pepper respectively by supercritical CO2 extraction process to obtain corresponding essential oils; (2) Extracting ginseng, patchouli, nutmeg, peppermint, mint, eucommia bark, ginger, perilla leaf, tangerine peel, clove leaf, clove bud, cinnamon, fennel, star anise, rhodiola rosea, salvia miltiorrhiza, ginkgo leaf, camphor bark, camphor leaf, frankincense, magnolia flower, eucalyptus leaf, calamus, rose, thyme, Roman chamomile, clove basil, lavender, wormwood, notopterygium wilfordii, smilax glabra, cassia twig, dipsacus root, and sambucus by steam distillation to obtain corresponding essential oils; (3) Vitamin E oil, menthol, ethanol, and grape seed oil as additives; Dissolve the ethanol and menthol in a weight ratio of 2:1 to obtain an ethanol-menthol solution for later use; (4) Mix and stir the essential oils with good liquid fluidity at a stirring speed of 10 rpm; (5) Continue to add essential oil with poor liquid fluidity and mix and stir at a stirring speed of 15-25 rpm for 45-60 min; (6) Adjust the speed to 10 rpm, add rose oil, storax oil, agarwood oil, sandalwood oil, benzoin oil, saffron oil, frankincense oil and ethanol menthol solution, and stir for 30 minutes; (7) Let it stand and then fill it; The analgesic effect is to relieve muscle pain caused by non-bone organic lesions due to inflammation or mechanical fatigue.

2. The external preparation according to claim 1, characterized in that Flavorings are also included.

3. The external preparation according to claim 2, characterized in that The flavoring agent is linalool thyme oil, Roman chamomile oil, jasmine essential oil, clary perilla oil, rose oil or lavender oil.

4. The method for preparing the external preparation of plant-extracted volatile oil with fatigue-relieving and analgesic effects as claimed in claim 1, characterized in that: The steps include: (1) Extracting angelica, angelica dahurica, Zanthoxylum bungeanum, saffron, atractylodes macrocephala, ligusticum chuanxiong, safflower, costusroot, cyperus rotundus, atractylodes lancea, magnolia officinalis, storax, agarwood, sandalwood, benzoin, angelica pubescens, dalbergia odorifera, myrrh, saposhnikovia divaricata, and pepper respectively by supercritical CO2 extraction process to obtain corresponding essential oils; (2) Extracting ginseng, patchouli, nutmeg, peppermint, mint, eucommia bark, ginger, perilla leaf, tangerine peel, clove leaf, clove bud, cinnamon, fennel, star anise, rhodiola rosea, salvia miltiorrhiza, ginkgo leaf, camphor bark, camphor leaf, frankincense, magnolia flower, eucalyptus leaf, calamus, rose, thyme, Roman chamomile, clove basil, lavender, wormwood, notopterygium wilfordii, smilax glabra, cassia twig, dipsacus root, and sambucus by steam distillation to obtain corresponding essential oils; (3) Vitamin E oil, menthol, ethanol, and grape seed oil as additives; Dissolve the ethanol and menthol in a weight ratio of 2:1 to obtain an ethanol-menthol solution for later use; (4) Mix and stir the essential oils with good liquid fluidity at a stirring speed of 10 rpm; (5) Continue to add essential oil with poor liquid fluidity and mix and stir at a stirring speed of 15-25 rpm for 45-60 min; (6) Adjust the speed to 10 rpm, add rose oil, storax oil, agarwood oil, sandalwood oil, benzoin oil, saffron oil, frankincense oil and ethanol menthol solution, and stir for 30 minutes; (7) Allow to stand and then fill.

5. The preparation method according to claim 4, characterized in that: The specific operation of the supercritical CO2 extraction process in step (1) is: (11) Washing and crushing the raw materials, and respectively putting them into a supercritical extraction kettle; (12) Cooling the storage tank and heating the extraction kettle and the analytical kettle at the same time. When the temperature is heated to 60-160°C, pressurizing the extraction kettle and the analytical kettle; (13) When the pressure reaches 5.0~30MPa, the cyclic extraction begins; (14) Maintain constant temperature and pressure at 60°C and 5.0 MPa for 2 hours to extract the volatile oil and collect it in a packaging container for later use.

6. The preparation method according to claim 4, characterized in that: The specific operation of the steam distillation extraction process in step (2) is: (21) Wash and crush the raw materials, place them in distillation tanks, and inject clean water; (22) The oil-water mixture vapor is formed by heating and pressurizing with steam, boiling and vaporizing at 700-1400 Pa and 100-120 °C; (23) obtaining a mixture of essential oil and water through a condenser; (24) Separate the oil and water to obtain the volatile oil, put it into an aluminum bottle, seal it and store it in a cool place for later use.

7. Use of the external preparation of plant-extracted volatile oil with fatigue-relieving and analgesic effects as claimed in claim 1 in the preparation of analgesic and fatigue-relieving drugs; The analgesia is to relieve the muscle pain of non-bone organic lesions caused by inflammation or mechanical fatigue.

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