Compositions containing gaba-gated chloride channel antagonists

Abstract

The present application relates to a composition containing a biologically effective amount of compound I and at least one GABA-gated chloride channel antagonist, and a method for preventing and treating pests in agronomic and non-agronomic environments. The composition of the present application has a synergistic effect, which can improve the effect of pest control, expand the insecticidal spectrum, and the occurrence of pests in the field is mixed, and the effect on pests is stronger.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of pesticide combinations, and specifically relates to a composition containing a biologically effective amount of compound I and at least one GABA-gated chloride channel antagonist, and a method for controlling pests in agronomic and non-agronomic environments. BACKGROUND

[0002] In recent years, the occurrence of various crop pests has shown a trend of increasing year by year, and the occurrence area has increased year by year, which has brought great harm to crops, resulting in crop yield reduction, agricultural production cost increase and other problems. In order to improve the control effect on pests, farmers increase the amount of pesticide or randomly mix pesticides. This unscientific use of pesticides not only does not achieve the effect of increasing efficiency, but also leads to problems such as waste of pesticides, excessive pesticide residues, pollution of the environment, and resistance of pests to pesticides. Therefore, it is urgent to develop a synergistic composition for pests, which can achieve the effects of expanding the range of control objects, reducing the amount of pesticide, and delaying the development of resistance.

[0003] In the actual process of agricultural production, long-term continuous use of the same pesticide will quickly lead to pesticide resistance of pests, resulting in reduced control effect, increased pesticide use, and aggravated pesticide residues in agricultural products and destruction of the ecological environment. By compounding with insecticides of completely different mechanisms, it is an effective way to delay the development of pesticide resistance, expand the insecticidal spectrum, prolong the service life, and reduce the amount of pesticide used. However, how to compound the drugs to produce synergistic rather than antagonistic effects is a major difficulty.

[0004] Patent document CN 111909143 A discloses compound I, which has broad-spectrum insecticidal activity, especially good control effect on pests such as Lepidoptera, Hemiptera, Coleoptera, Thysanoptera, Diptera, mites, etc. However, it does not disclose how to compound it with other drugs to achieve the purpose of synergism.

[0005] The structural formula of compound I is as follows,

[0006] SUMMARY

[0007] To improve the deficiencies of the prior art, the present application provides a composition comprising compound I and a GABA-gated chloride channel antagonist selected from at least one of the following compounds: broflanilide, 1-(2,6-dichloro-4-(trifluoromethyl)phenyl)-4-((difluoromethyl)thio)-5-((pyridin-2-ylmethyl)amino)-1H-pyrazole-3-carbonitrile (CAS: 394730-71-3), isothioiclofen, cycloprothrin, fluridone, afidopyropen, fluxametamide, sifentra, 1-(2,6-dichloro-a,a,a-trifluoro-p-tolyl)-4-(fluoromethylthio)-5-[(pyrazinylmethyl)amino]pyrazole-3-carbonitrile (CAS: 315208-17-4), lotilifanide;

[0008] The structural formula of the compound I is as follows,

[0009]

[0010] According to an embodiment of the present application, the compound I is prepared by referring to the method of Example 1 in the patent document CN 111909143 A, the whole of which is incorporated herein by reference.

[0011] According to an embodiment of the present application, the mass ratio of the compound I and the GABA-gated chloride channel antagonist is 80-1:1-80.

[0012] According to an embodiment of the present application, the mass ratio of the compound I and the GABA-gated chloride channel antagonist is 50-1:1-50, for example 50:1, 40:1, 30:1, 20:1, 10:1, 5:1, 4:1, 3:1, 2:1, 1:1, 1:2, 1:3, 1:4, 1:5, 1:10, 1:20, 1:30, 1:40 or 1:50.

[0013] According to a preferred embodiment of the present application, the mass ratio of the compound I and the GABA-gated chloride channel antagonist is 10-1:1-10.

[0014] According to an embodiment of the present application, the sum of the mass of the compound I and the GABA-gated chloride channel antagonist in the composition is 1-80%, preferably 2-70%, or 5-65%, 10-60%, for example 1%, 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45% or 50%, based on the total weight of the composition being 100%.

[0015] According to an embodiment of the present application, the composition can be prepared into a liquid preparation or a solid preparation.

[0016] According to the embodiments of the present application, the content of the effective active ingredient in the formulation varies according to different formulation types. Generally, the content of the effective active ingredient in the liquid formulation is 1% to 60% by weight, preferably 5% to 50% by weight; the content of the effective active ingredient in the solid formulation is 5% to 80% by weight, preferably 10% to 70% by weight.

[0017] According to the embodiments of the present application, the composition further comprises at least one of deionized water, organic solvent, emulsifier, dispersant, wetting agent, thickening agent, antifoaming agent, stabilizer, binder, disintegrant, anti-freezing agent, anti-caking agent, suspending agent, film-forming agent, preservative, colorant, high molecular capsule wall material, pH adjustor, or filler, etc.

[0018] According to the embodiments of the present application, the composition can be diluted by the user before use or used directly. The preparation thereof can be prepared by mixing the effective active ingredient with one or more of deionized water, organic solvent, emulsifier, dispersant, wetting agent, thickening agent, antifoaming agent, stabilizer, binder, disintegrant, anti-freezing agent, anti-caking agent, suspending agent, film-forming agent, preservative, colorant, high molecular capsule wall material, pH adjustor, or filler, etc., to obtain the composition.

[0019] According to the embodiments of the present application, the composition can be prepared into various dosage forms. Preferably, the dosage forms include wettable powder, water dispersible granule, suspension concentrate, emulsion, suspension seed coating, microcapsule suspension, microcapsule suspension-suspension, emulsifiable concentrate, microemulsion, dispersible liquid, granule.

[0020] According to the embodiments of the present application, when the composition is prepared into wettable powder, the composition comprises the following components and contents: compound I 1% to 80%, GABA-gated chloride channel antagonist 1% to 80%, dispersant 0% to 10%, wetting agent 0% to 8%, and the balance is filler.

[0021] Preferably, the content of compound I in the composition is 2% to 70%, 5% to 65%, 10% to 60%, 15% to 50%, etc.

[0022] The content of GABA-gated chloride channel antagonist is 2% to 70%, 5% to 65%, 10% to 60%, 15% to 50%, etc.

[0023] The content of dispersant is 1% to 8%, 2% to 7%, 3-6%, or 4% to 5%.

[0024] The content of wetting agent is 1% to 8%, 2% to 7%, 3-6%, or 4% to 5%.

[0025] According to an embodiment of the present application, the composition, when formulated as a water dispersible granule, comprises the following components and amounts: Compound I 1% to 80%, a GABA-gated chloride channel antagonist 1% to 80%, a dispersing agent 0% to 10%, a wetting agent 0% to 16%, a disintegrant 0% to 20%, and the balance being a filler.

[0026] Preferably, Compound I in the composition is 2% to 70%, 5% to 65%, 10% to 60%, 15% to 50%, etc.

[0027] The GABA-gated chloride channel antagonist is 2% to 70%, 5% to 65%, 10% to 60%, 15% to 50%, etc.

[0028] The dispersing agent is 1% to 8%, 2% to 7%, 3-6%, or 4% to 5%.

[0029] The wetting agent is 1% to 8%, 2% to 7%, 3-6%, or 4% to 5%.

[0030] The disintegrant is 1% to 18%, 2% to 16%, 3-15%, 4% to 12%, 5% to 10%, or 6-8%.

[0031] According to an embodiment of the present application, the composition, when formulated as a suspension concentrate, comprises the following components and amounts: Compound I 1% to 50%, a GABA-gated chloride channel antagonist 1% to 50%, a dispersing agent 0% to 5%, a wetting agent 0% to 8%, a thickening agent 0% to 0.3%, an anti-freeze agent 0% to 5%, and the balance being deionized water.

[0032] Preferably, Compound I in the composition is 2% to 45%, 5% to 40%, 10% to 35%, 15% to 30%, 20% to 25%, etc.

[0033] The GABA-gated chloride channel antagonist is 2% to 45%, 5% to 40%, 10% to 35%, 15% to 30%; 20% to 25%, etc.

[0034] The dispersing agent is 1% to 4% or 2% to 3%.

[0035] The wetting agent is 1% to 8%, 2% to 7%, 3-6%, or 4% to 5%.

[0036] The thickening agent is 0.1% to 0.25%.

[0037] The anti-freeze agent is 1% to 4% or 2% to 3%.

[0038] According to an embodiment of the present application, the composition, when formulated as a water emulsion, comprises the following components and amounts: Compound I 1-50%, GABA-gated chloride channel antagonist 1-50%, organic solvent 0-95%, emulsifier 0-10%, anti-freezing agent 0-5%, antifoaming agent 0-0.2%, thickening agent 0-0.5%, and the balance being deionized water.

[0039] Preferably, Compound I in the composition is 2-45%, 5-40%, 10-35%, 15-30%, 20-25%, etc.

[0040] The GABA-gated chloride channel antagonist is 2-45%, 5-40%, 10-35%, 15-30%, 20-25%, etc.

[0041] The organic solvent is 1-90%, 2-80%, 5-70%, 10-60%, 15-50%, 20-50%, 30-40%, etc.

[0042] The emulsifier is 1-8%, 2-7%, 3-6%, or 4-5%.

[0043] The anti-freezing agent is 1-4% or 2-3%.

[0044] The antifoaming agent is 0.05-0.1%, or

[0045] The thickening agent is 0.1-0.4%, 0.2-0.3%.

[0046] According to an embodiment of the present application, the composition, when formulated as a suspension seed coating agent, comprises the following components and amounts: Compound I 1-50%, GABA-gated chloride channel antagonist 1-50%, dispersing agent 1-12%, wetting agent 1-10%, anti-freezing agent 1-10%, anti-caking agent 0.1-10%, suspending agent 0.1-5%, film-forming agent 1-10%, preservative 0.1-5%, coloring agent 1-30%, pH adjuster 0.1-5%, thickening agent 0.1-8%, and the balance being deionized water.

[0047] Preferably, Compound I in the composition is 2-45%, 5-40%, 10-35%, 15-30%, 20-25%, etc.

[0048] The GABA-gated chloride channel antagonist is 2-45%, 5-40%, 10-35%, 15-30%, 20-25%, etc.

[0049] The dispersing agent is 2-10%, 3-8%, 4-7%, or 5-6%.

[0050] The wetting agent is 1-8%, 2-7%, 3-6%, or 4-5%;

[0051] The anti-freezing agent is 1-8%, 2-7%, 3-6%, or 4-5%;

[0052] The anti-caking agent is 0.5-9%, 1-8%, 2-7%, 3-6%, or 4-5%;

[0053] The suspending agent is 0.2-4%, 0.5-3.5%, 1-3%, 1.5-2.5%, or 1.8-2%;

[0054] The film-forming agent is 2-9%, 3-8%, 4-7%, or 5-6%;

[0055] The preservative is 0.2-4%, 0.5-3.5%, 1-3%, 1.5-2.5%, or 1.8-2%;

[0056] The coloring agent is 2-25%, 3-20%, 5-15%, 8-12%, or 9-10%;

[0057] The pH regulator is 0.1-5%, 0.2-4%, 0.5-3.5%, 1-3%, 1.5-2.5%, or 1.8-2%;

[0058] The thickening agent is 0.5-7, 1-6%, 2-5%, or 3-4%.

[0059] According to an embodiment of the present application, when the composition is made into a microcapsule suspension agent, it comprises the following components and contents: Compound I 1-50%, GABA-gated chloride channel antagonist 1-50%, high molecular capsule wall material 1-30%, dispersant 2-10%, organic solvent 1-50%, emulsifier 1-7%, pH regulator 0.1-5%, antifoaming agent 0.01-2%, thickening agent 0.1-8%, anti-freezing agent 0.1-8%, and the balance is deionized water.

[0060] Preferably, Compound I in the composition is 2-45%, 5-40%, 10-35%, 15-30%, 20-25%, etc.

[0061] The GABA-gated chloride channel antagonist is 2-45%, 5-40%, 10-35%, 15-30%, 20-25%, etc.

[0062] The high molecular capsule wall material is 2-25%, 5-20%, 6-18%, or 10-15%, etc.

[0063] The dispersant is 3% to 8%, 4% to 7%, or 5% to 6%;

[0064] The organic solvent is 2% to 45%, 5% to 40%, 10% to 35%, 15% to 30%; or 20% to 25%, etc.

[0065] The emulsifier is 2% to 6% or 4% to 5%;

[0066] The pH regulator is 0.2% to 4%, 0.5% to 3.5%, 1% to 3%, 1.5% to 2.5%, or 1.8% to 2%;

[0067] The antifoaming agent is 0.05% to 1.8%, 0.1% to 1.5%, 0.5% to 1.3%, 0.8% to 1.0%;

[0068] The thickening agent is 0.2% to 7, 0.5% to 6%, 1% to 5%, 1.5% to 4%, or 2% to 3%;

[0069] The antifreeze agent is 0.5% to 7%, 1% to 6%, 2% to 5%, or 3% to 4%.

[0070] According to an embodiment of the present application, when the composition is made into a microcapsule suspension-suspension agent, it comprises the following components and contents: Compound I 1% to 50%, GABA-gated chloride channel antagonist 1% to 50%, high molecular capsule wall material 1% to 12%, dispersant 1% to 12%, wetting agent 1% to 8%, organic solvent 1% to 50%, emulsifier 1% to 8%, antifoaming agent 0.01% to 2%, thickening agent 0.1% to 8%, pH regulator 0.1% to 5%, antifreeze agent 0.1% to 8%, and the balance is deionized water.

[0071] According to an embodiment of the present application, when the composition is made into an emulsifiable concentrate, it comprises the following components and contents: Compound I 1% to 50%, GABA-gated chloride channel antagonist 1% to 50%, emulsifier 0% to 10%, antifreeze agent 0% to 5%, stabilizer 0% to 0.5%, and the balance is organic solvent.

[0072] Alternatively, Compound I 1% to 50%, GABA-gated chloride channel antagonist 1% to 50%, emulsifier 0% to 12%, stabilizer 0% to 0.5%, and the balance is organic solvent.

[0073] According to an embodiment of the present application, when the composition is made into a microemulsion, it comprises the following components and contents: Compound I 1% to 50%, GABA-gated chloride channel antagonist 1% to 50%, organic solvent 1% to 50%, emulsifier 1% to 30%, antifreeze agent 1% to 10%, stabilizer 0.1% to 5%, and the balance is deionized water.

[0074] According to the embodiment of the present application, the composition, when made into a dispersible solution, comprises the following components and contents: Compound I 1% to 50%, GABA-gated chloride channel antagonist 1% to 50%, emulsifier 1 to 30%, anti-freezing agent 1% to 10%, stabilizer 0.1% to 5%, and the rest is organic solvent.

[0075] According to the embodiment of the present application, the composition, when made into a granule, comprises the following components and contents: Compound I 0.1% to 10%, GABA-gated chloride channel antagonist 0.1% to 10%, dispersant 0% to 5%, wetting agent 0% to 5%, binder 0% to 3%, and the rest is filler.

[0076] According to the embodiment of the present application, the emulsifier can be at least one selected from the group consisting of sodium lignosulfonate, agricultural emulsifier, phenyl phenol polyoxyethyl ether phosphate, triphenyl ethyl styryl phenol polyoxyethylene ether phosphate triethanolamine salt, benzyl dimethyl phenol polyoxyethyl ether, sorbitan fatty acid ester polyoxyethylene ether, sorbitan oleate (Span-80), fatty alcohol polyoxyethylene ether, sodium alkyl naphthalene sulfonate, isooctanol succinate sodium sulfonate, nonyl phenol polyoxyethylene ether phosphate, and castor oil polyoxyethylene ether phosphate.

[0077] According to the embodiment of the present application, the dispersant can be at least one selected from the group consisting of glycerol fatty acid polyoxyethylene ether, polyoxyethylene alkyl aryl ether, sodium lignosulfonate, naphthalene sulfonate formaldehyde condensate, fatty alcohol polyoxyethylene ether sulfate, naphthalene sulfonate formaldehyde condensate, naphthalene sulfonate formaldehyde condensate sodium salt, nonyl phenol polyoxyethylene ether, polyoxyethylene lanolin alcohol, alkyl phenol polyoxyethylene ether formaldehyde condensate, fatty alcohol polyoxyethylene ether, fatty alcohol polyoxyethylene ether phosphate, polyoxyethylene sorbitan fatty acid ester, and phosphate.

[0078] According to the embodiment of the present application, the wetting agent can be at least one selected from the group consisting of trisiloxane polyoxyethylene ether, sodium N-lauroyl glutamate, sodium dodecyl sulfate, sodium lauroyl sarcosinate, sodium methyl naphthalene sulfonate formaldehyde condensate, castor oil polyoxyethylene ether, triphenyl ethyl phenol polyoxyethylene ether, sodium dodecyl benzene sulfonate, sodium alkyl naphthalene sulfonate, isooctanol succinate sodium sulfonate, polyoxyethylene alkyl aryl ether, fatty alcohol polyether glycerol fatty acid polyoxyethylene ether, and fatty alcohol polyoxyethylene ether.

[0079] According to the embodiment of the present application, the binder can be at least one selected from the group consisting of xanthan gum, starch, urea formaldehyde resin, gelatin, gum arabic, carboxymethyl cellulose, carboxyethyl cellulose, and polyvinyl alcohol.

[0080] According to the embodiment of the present application, the disintegrant can be at least one selected from the group consisting of sodium bicarbonate, ammonium sulfate, sodium sulfate, calcium sulfate, and magnesium chloride.

[0081] According to an embodiment of the present application, the thickening agent can be selected from at least one of magnesium aluminum silicate, polyvinyl acetate, xanthan gum, gelatin, gum arabic, and polyvinyl alcohol.

[0082] According to an embodiment of the present application, the antifoaming agent can be selected from at least one of silicone oil, n-octanol, silicone, butyl phosphate, isobutyl phosphate, and the like.

[0083] According to an embodiment of the present application, the antifreezing agent can be selected from at least one of propylene glycol, ethylene glycol, glycerol, and the like.

[0084] According to an embodiment of the present application, the stabilizer can be selected from at least one of triethanolamine, epichlorohydrin, butyl glycidyl ether, triphenyl phosphite, N-soybean oil-based triethylene diamine, and dialkyl succinate acid sulfonate.

[0085] According to an embodiment of the present application, the filler includes a solid filler and a liquid filler, wherein the solid filler can be selected from at least one of kaolin, attapulgite, diatomite, white carbon, bentonite, montmorillonite, calcium carbonate, and talc powder. The liquid filler can be selected from at least one of soybean oil, castor oil, and mineral oil.

[0086] According to an embodiment of the present application, the organic solvent can be selected from at least one of ethyl acetate, acetone, isopropyl alcohol, 2,2,2-trifluoroethanol, propylene carbonate, benzene, toluene, xylene, dimethylformamide, dimethyl sulfoxide, dichloromethane, cyclohexane, cyclohexanone, N-methyl pyrrolidone, and solvent oil (e.g., 150# solvent oil).

[0087] The present application also provides a use of the above composition for controlling pests in agriculture and non-agriculture.

[0088] According to an embodiment of the present application, the pests are selected from Lepidoptera, Hemiptera, Coleoptera, Thysanoptera, Diptera, mites, and the like.

[0089] As an embodiment, the pests are selected from the group consisting of Plutella xylostella, Ostrinia nubilalis, Spodoptera exigua, Phyllotreta striolata (Phyllotreta striola).

[0090] The present application also provides a method for controlling pests in agriculture or non-agriculture, which comprises applying the above composition to plants where the pests exist.

[0091] The composition of the present application can be provided in the form of finished preparation, or in the form of single agent, which is mixed directly before use, then mixed with water to the required concentration, and can be applied to the crop in any way, such as spraying, irrigation at the root of the plant, smearing, etc. In specific application, it can also be mixed with other agents, such as growth regulator, soil conditioner, herbicide, nematicide, etc.

[0092] The present application also provides a composition for controlling invertebrate pests, which comprises a biologically effective amount of compound I, a GABA-gated chloride channel antagonist and at least one further component selected from the group consisting of surfactants, solid diluents and liquid diluents, said composition optionally further containing an effective amount of at least one further biologically active compound or active agent, said GABA-gated chloride channel antagonist being selected from at least one of the following compounds: abamectin, 1-(2,6-dichloro-4-(trifluoromethyl)phenyl)-4-((difluoromethyl)thio)-5-((pyridin-2-ylmethyl)amino)-1H-pyrazole-3-carbonitrile (CAS: 394730-71-3), amidoflumet, cyromazine, fluralaner, flupropadine, fluxametamide, saku- lana, 1-(2,6-dichloro-a,a,a-trifluoro-p-tolyl)-4-(fluoromethylthio)-5-[(pyrazinylmethyl)amino]pyrazole-3-carbonitrile (CAS: 315208-17-4), lotilaner.

[0093] The present application also provides a method for controlling invertebrate pests, which comprises contacting said invertebrate pests or their environment with a biologically effective amount of a composition of the present application as described above.

[0094] The present application also provides a spray composition, which comprises a composition of the present application as described above and a propellant.

[0095] The present application also provides a bait composition, which comprises a composition of the present application as described above; one or more food materials; optionally an attractant; and optionally a humectant.

[0096] Advantages of the present application:

[0097] The synergistic composition of the present application has the following advantages:

[0098] 1) synergistic effect, which can improve the control effect of pests; 2) expand the insecticidal spectrum, mixed occurrence of field pests, stronger effect on pests; 3) different action mechanisms of the two effective components, mixed use can delay the development of pest resistance; 4) reduce the amount of application, reduce the use frequency, and reduce the use cost. DETAILED DESCRIPTION

[0099] The technical solutions of the present application will be further described in detail below in combination with specific embodiments. It should be understood that the following embodiments are only illustrative and explanatory of the present application, and should not be interpreted as limiting the scope of protection of the present application. Any technology implemented based on the above description of the present application is included in the scope of protection intended by the present application.

[0100] Unless otherwise specified, the raw materials and reagents used in the following examples are commercially available or can be prepared by known methods.

[0101] The present application uses a combination of indoor bioassay and field test to test the insecticidal effect of the composition. It should be noted that any modification, equivalent replacement and improvement within the spirit and principles of the present application should be included in the scope of protection of the present application.

[0102] The compound I used in the following examples was prepared according to the method of Example 1 in the patent document CN 111909143 A.

[0103] The % of each component in the formulations of the following examples, if not specifically stated, refers to the mass percentage content.

[0104] I. Preparation Example:

[0105] Preparation Example 1: Wettable powder

[0106]

[0107]

[0108] The above materials are roughly crushed in proportion and then mixed uniformly in a mixer. After air flow crushing, the finished product is obtained.

[0109] Preparation Example 2: Water dispersible granule

[0110]

[0111] The effective active ingredient and various adjuvants are mixed uniformly in proportion to the formula. After air flow crushing, the powder is obtained. A certain amount of water is added to mix and extrude granulation. After drying and screening, the finished product is obtained.

[0112] Preparation Example 3: Suspension concentrate

[0113]

[0114] The effective active ingredient and various adjuvants are mixed uniformly in proportion to the formula. After high-speed shearing and sand milling, the finished product is obtained.

[0115] Preparation Example 4: Emulsifiable concentrate

[0116]

[0117] The above raw materials are mixed in proportion to be dissolved into a uniform oil phase; after passing the inspection, metering and packaging are performed to be finished products.

[0118] Preparation Example 5: water emulsion

[0119]

[0120] The above raw materials are added into a batching kettle according to the requirements of the formula, mixed uniformly by a high-speed shearing machine to prepare a water emulsion, which, after passing the inspection, is metered and packaged to be finished products.

[0121] Preparation Example 6: emulsifiable concentrate

[0122]

[0123] The above raw materials are mixed in proportion to be dissolved into a uniform oil phase; after passing the inspection, metering and packaging are performed to be finished products.

[0124] Preparation Example 7: granules

[0125]

[0126] The components are fully mixed according to the formula proportion, crushed, wetted with water, fully stirred again, granulated by a screw extrusion granulator, dried, and sieved to obtain finished products.

[0127] The GABA-gated chloride channel antagonist is selected from at least one of the following compounds: bromofenprox, 1-(2,6-dichloro-4-(trifluoromethyl)phenyl)-4-((difluoromethyl)thio)-5-((pyridin-2-ylmethyl)amino)-1H-pyrazole-3-carbonitrile (CAS: 394730-71-3), isoxadifen-ethyl, cyenopyrafen, fluridone, abamectin, fluxametamide, saroche, 1-(2,6-dichloro-a,a,a-trifluoro-p-tolyl)-4-(fluoromethylthio)-5-[(pyrazinylmethyl)amino]pyrazole-3-carbonitrile (CAS: 315208-17-4), lufenuron, as described in the above preparation examples.

[0128] II. Indoor toxicity determination examples

[0129] (1) Test insect: Plutella xylostella (Linnaeus), Mythimna separata Walker, Spodoptera exigua (Hübner), Phyllotreta vittuta Fabr.

[0130] (2) Assay method:

[0131] Pest toxicity assay method

[0132] The pest toxicity assay takes cabbage moths, corn stalk borers, cabbage beetles, and other pests as test materials, and uses the leaf dipping method to determine the toxicity of compound I, GABA-gated chloride channel antagonists, and their mixed formulations.

[0133] According to the Chinese Agricultural Industry Standard NY / T 1154.14-2008 "Guidelines for Indoor Biological Assay of Pesticides", Part 14: Leaf Dipping Method, the test agent (including compound I, GABA-gated chloride channel antagonists) is first prepared into 5 concentration gradients (according to the LC 50 values of the compounds for different pests, the concentration gradients are set according to the ratio or difference) using a suitable solvent (the types of solvents such as acetone, methanol, N,N dimethylformamide, and dimethyl sulfoxide, etc., are selected according to their solubility in the sample). 50 The compound I, GABA-gated chloride channel antagonists to be mixed are set in a series of proportions according to their LC 50 values in mass proportion, and are prepared into final mass concentrations (referring to the total mass of compound I, GABA-gated chloride channel antagonists) according to different proportions.

[0134] The leaf discs or leaf segments are immersed in the test agent solution, and after 10 seconds (the immersion time can be appropriately extended or shortened according to the characteristics of the agent), they are taken out and dried, placed in a culture dish containing 1% water agar or moisture-retaining filter paper, and the test insects are introduced, with not less than 10 individuals per repetition. Each treatment has not less than 4 repetitions, and a treatment without the agent (containing all organic solvents and emulsifiers) is set as a blank control.

[0135] The co-toxicity coefficient (CTC value) of the two agents in different proportions is calculated according to the Sun Yunpei method. The co-toxicity coefficient (CTC) of the complex agent is ≥120, indicating synergistic effect, ≤80 indicating antagonistic effect, and 80<CTC<120 indicating additive effect. The co-toxicity coefficient (CTC) of the mixed agent is calculated according to formula (1), formula (2), and formula (3):

[0136]

[0137] In the formula:

[0138] ATI - measured toxicity index of the mixed agent;

[0139] S - LC 50 of the standard insecticide, in milligrams per liter (mg / L);

[0140] M - LC 50 of the mixed agent, in milligrams per liter (mg / L)

[0141] TTI = TI A x P A + TI B x P B (2)

[0142] In the formula:

[0143] TTI - theoretical toxicity index of the mixture;

[0144] TI A - toxicity index of A agent;

[0145] P A - percentage content of A agent in the mixture, in percentage (%);

[0146] TI B - toxicity index of B agent;

[0147] P B - percentage content of B agent in the mixture, in percentage (%).

[0148]

[0149] In the formula:

[0150] CTC - coefficient of toxicity;

[0151] ATI - actual toxicity index of the mixture;

[0152] TTI - theoretical toxicity index of the mixture.

[0153] After mixing of different varieties of pesticides, three types of action, i.e. additive action, synergistic action and antagonistic action, are usually exhibited, but the specific action cannot be predicted. The composition described in the present application uses compound I and GABA-gated chloride channel antagonist as effective active ingredients, and is illustrated by biological determination examples.

[0154] Laboratory determination of insecticidal activity of the insecticidal composition

[0155] In this test, the leaf dipping method was used to determine the activity of the insecticidal composition of Examples 1-3 on the cabbage moth.

[0156] Example 1: To verify the effect of different proportions of compound I and chlorantraniliprole on the cabbage moth, the indoor toxicity determination of different proportions of compound I and chlorantraniliprole on the cabbage moth was carried out. The corresponding concentrations were prepared according to the mass proportions in the table, and the indoor insecticidal test was carried out, and the test results are as follows:

[0157] Table 1 Toxicity determination results of compound I and chlorantraniliprole single agent and their mixture on the cabbage moth

[0158]

[0159]

[0160] From the results of Table 1, it can be seen that the mixture of compound I and brofenvalerate in different proportions has obvious synergistic effect on the activity against Plutella xylostella.

[0161] Example 2: To verify the effect of compound I and 1-(2,6-dichloro-4-(trifluoromethyl)phenyl)-4-((difluoromethyl)thio)-5-((pyridin-2-ylmethyl)amino)-1H-pyrazole-3-carbonitrile in different proportions on Plutella xylostella, indoor toxicity determination of compound I and 1-(2,6-dichloro-4-(trifluoromethyl)phenyl)-4-((difluoromethyl)thio)-5-((pyridin-2-ylmethyl)amino)-1H-pyrazole-3-carbonitrile in different proportions on Plutella xylostella was carried out, and the corresponding concentration was prepared according to the mass proportion in the table, and indoor insecticidal test was carried out. The test results are as follows:

[0162] Table 2 Toxicity determination results of single agent and its mixture of compound I and 1-(2,6-dichloro-4-(trifluoromethyl)phenyl)-4-((difluoromethyl)thio)-5-((pyridin-2-ylmethyl)amino)-1H-pyrazole-3-carbonitrile on Plutella xylostella

[0163]

[0164]

[0165] From the results of Table 2, it can be seen that the mixture of compound I and 1-(2,6-dichloro-4-(trifluoromethyl)phenyl)-4-((difluoromethyl)thio)-5-((pyridin-2-ylmethyl)amino)-1H-pyrazole-3-carbonitrile in different proportions has obvious synergistic effect on the activity against Plutella xylostella.

[0166] Example 3: To verify the effect of compound I and isoxazoline amide in different proportions on Plutella xylostella, indoor toxicity determination of compound I and isoxazoline amide in different proportions on Plutella xylostella was carried out, and the corresponding concentration was prepared according to the mass proportion in the table, and indoor insecticidal test was carried out. The test results are as follows:

[0167] Table 3 Toxicity determination results of single agent and its mixture of compound I and isoxazoline amide on Plutella xylostella

[0168] Active ingredient Ratio Regression equation LC 50 ]]> Co-toxicity factor Evaluation Compound I — y = 6.8787 + 2.1062x 0.1282 — — isocycloseram — y = 5.2578 + 1.3784x 0.6501 — — Compound I: isocycloseram 50:1 y = 6.6079 + 1.5777x 0.0957 136.1018 Synergism Compound I: isocycloseram 10:1 y = 6.6734 + 1.5434x 0.0824 167.8298 Synergism Compound I: isocycloseram 1:1 y = 6.5402 + 1.5425x 0.1004 213.3131 Synergism Compound I: isocycloseram 1:10 y = 5.5442 + 1.1418x 0.3337 142.1958 Synergism Compound I: isocycloseram 1:50 y = 5.4491 + 1.3134x 0.4551 132.2920 Synergism

[0169] From the results of Table 3, it can be seen that the mixture of compound I and isoxazoline amide in different proportions has obvious synergistic effect on the activity against Plutella xylostella.

[0170] Insecticidal activity determination test of corn borer in laboratory

[0171] In this test, leaf dipping method was used to determine the activity of the insecticidal compositions of Examples 4-6 on corn borer.

[0172] Example 4: To verify the effect of different proportions of compound I and cyflumetofen on corn borer, indoor toxicity determination of different proportions of compound I and cyflumetofen on corn borer was carried out, and the corresponding concentrations were prepared according to the mass ratio in the table, and indoor insecticidal test was carried out, and the test results were as follows:

[0173] Table 4 Toxicity determination results of compound I and cyflumetofen single agent and its mixture on corn borer

[0174]

[0175]

[0176] From the results in Table 4, the activity of different proportions of compound I and cyflumetofen mixed for corn borer has obvious synergistic effect.

[0177] Example 5: To verify the effect of different proportions of compound I and fluralaner on corn borer, indoor toxicity determination of different proportions of compound I and fluralaner on corn borer was carried out, and the corresponding concentrations were prepared according to the mass ratio in the table, and indoor insecticidal test was carried out, and the test results were as follows:

[0178] Table 5 Toxicity determination results of compound I and fluralaner single agent and its mixture on corn borer

[0179] Active ingredient Ratio Regression equation LC 50 ]] Co-toxicity factor Evaluation Compound I — y = 5.7178 + 2.0073x 0.4389 — — fluralaner — y = 5.1603 + 1.3749x 0.7646 — — Compound I: fluralaner 50:1 y = 5.5581 + 1.2095x 0.3456 128.0658 Synergism Compound I: fluralaner 10:1 y = 5.7351 + 1.3008x 0.2722 167.7367 Synergism Compound I: fluralaner 1:1 y = 5.9468 + 1.4927x 0.2321 240.2750 Synergism Compound I: fluralaner 1:10 y = 5.3320 + 1.2316x 0.5376 133.2372 Synergism Compound I: fluralaner 1:50 y = 5.3257 + 1.2993x 0.5615 134.2188 Synergism

[0180] From the results in Table 5, the activity of different proportions of compound I and fluralaner mixed for corn borer has obvious synergistic effect.

[0181] Example 6: To verify the effect of different proportions of compound I and afugmentan on corn borer, indoor toxicity determination of different proportions of compound I and afugmentan on corn borer was carried out, and the corresponding concentrations were prepared according to the mass ratio in the table, and indoor insecticidal test was carried out, and the test results were as follows:

[0182] Table 6 Toxicity determination results of compound I and afugmentan single agent and its mixture on corn borer

[0183] Active ingredient Ratio Regression equation LC 50 ]]> Co-toxicity factor Evaluation Compound I — y = 5.7178 + 2.0073x 0.4389 — — afugamostat — y = 3.1283 + 1.5020x 17.6229 — — Compound I: afugamostat 50:1 y = 5.6552 + 1.4367x 0.3499 127.8799 Synergism Compound I: afugamostat 10:1 y = 5.6961 + 1.3607x 0.3079 156.4098 Synergism Compound I: afugamostat 1:1 y = 5.5067 + 1.2949x 0.4062 210.8492 Synergism Compound I: afugamostat 1:10 y = 4.4662 + 1.4272x 2.3661 163.3725 Synergism Compound I: afugamostat 1:50 y = 3.9029 + 1.3061x 6.9188 144.1169 Synergism

[0184] From the results in Table 6, the activity of different proportions of compound I and afugmentan mixed for corn borer has obvious synergistic effect.

[0185] Insecticidal activity determination test of corn borer in laboratory

[0186] The leaf-dipping method was used to determine the activity of the insecticidal compositions of Examples 7-8 against Helicoverpa armigera.

[0187] Example 7: To verify the effect of different proportions of Compound I and fluoxastro-mide on Helicoverpa armigera, indoor toxicity determination of different proportions of Compound I and fluoxastromide on Helicoverpa armigera was carried out. The corresponding concentrations were prepared according to the mass proportions in the table, and indoor insecticidal tests were carried out. The test results are as follows:

[0188] Table 7 Toxicity determination results of Compound I and fluoxastromide single agent and their mixtures on Helicoverpa armigera

[0189] Active ingredient Ratio Regression equation LC 50 ]]> Co-toxicity factor Evaluation Compound I — y = 6.9486 + 1.7989x 0.0826 — — fluoxazamide — y = 4.7549 + 2.0854x 1.3108 — — Compound I: fluoxazamide 50：1 y = 6.3673 + 1.1679x 0.0675 124.6597 Synergism Compound I: fluoxazamide 10：1 y = 6.6695 + 1.3292x 0.0555 162.6850 Synergism Compound I: fluoxazamide 5：1 y = 6.6629 + 1.2826x 0.0505 193.8415 Synergism Compound I: fluoxazamide 3：1 y = 6.4573 + 1.1853x 0.0589 183.1368 Synergism Compound I: fluoxazamide 1：1 y = 6.5124 + 1.2727x 0.0648 239.8257 Synergism Compound I: fluoxazamide 1：3 y = 6.1482 + 1.4488x 0.1612 172.3760 Synergism Compound I: fluoxazamide 1：5 y = 5.9580 + 1.3398x 0.1927 195.5407 Synergism Compound I: fluoxazamide 1：10 y = 5.5584 + 1.2949x 0.3705 150.4465 Synergism Compound I: fluoxazamide 1：50 y = 5.2056 + 1.3251x 0.6996 145.0819 Synergism

[0190] As can be seen from Table 7, the activity of different proportions of Compound I and fluoxastromide mixed against Helicoverpa armigera all has obvious synergistic effect.

[0191] Example 8: To verify the effect of different proportions of Compound I and sarolaner on Helicoverpa armigera, indoor toxicity determination of different proportions of Compound I and sarolaner on Helicoverpa armigera was carried out. The corresponding concentrations were prepared according to the proportions in the table, and indoor insecticidal tests were carried out. The test results are as follows:

[0192] Table 8 Toxicity determination results of Compound I and sarolaner single agent and their mixtures on Helicoverpa armigera

[0193] Active ingredient Ratio Regression equation LC 50 ]]> Co-toxicity factor Evaluation Compound I — y = 6.9486 + 1.7989x 0.0826 — — sarolaner — y = 4.0169 + 1.2584x 6.0432 — — Compound I: sarolaner 50:1 y = 6.7039 + 1.4017x 0.0609 138.3059 Synergism Compound I: sarolaner 10:1 y = 6.6227 + 1.4054x 0.0700 129.6216 synergism Compound I: sarolaner 1:1 y = 6.5499 + 1.4117x 0.0798 204.2261 synergism Compound I: sarolaner 1:10 y = 5.3780 + 1.3225x 0.5179 154.3566 synergism Compound I: sarolaner 1:50 y = 4.5229 + 1.6110x 1.9777 126.5613 synergism

[0194] As can be seen from Table 8, the activity of different proportions of Compound I and sarolaner mixed against Helicoverpa armigera all has obvious synergistic effect.

[0195] Indoor insecticidal activity determination test of Phyllotreta cruciferae

[0196] The leaf-dipping method was used to determine the activity of the insecticidal compositions of Examples 9-10 against Phyllotreta cruciferae.

[0197] Example 9: To verify the effect of different proportions of Compound I and 1-(2,6-dichloro-a,a,a-trifluoro-p-tolyl)-4-(fluoromethylthio)-5-[(pyrazinylmethyl)amino]pyrazole-3-carbonitrile on Phyllotreta cruciferae, indoor toxicity determination of different proportions of Compound I and 1-(2,6-dichloro-a,a,a-trifluoro-p-tolyl)-4-(fluoromethylthio)-5-[(pyrazinylmethyl)amino]pyrazole-3-carbonitrile on Phyllotreta cruciferae was carried out. The corresponding concentrations were prepared according to the mass proportions in the table, and indoor insecticidal tests were carried out. The test results are as follows:

[0198] Table 9 Toxicity test results of compound I and 1-(2,6-dichloro-a,a,a-trifluoro-p-tolyl)-4-(fluoromethylthio)-5-[(pyrazinylmethyl)amino]pyrazole-3-carbonitrile and their mixtures on Phyllotreta striolata

[0199]

[0200] As shown in Table 9, the different proportions of compound I and 1-(2,6-dichloro-a,a,a-trifluoro-p-tolyl)-4-(fluoromethylthio)-5-[(pyrazinylmethyl)amino]pyrazole-3-carbonitrile have obvious synergistic effect on Phyllotreta striolata.

[0201] Example 10: To verify the effect of different proportions of compound I and lotilaner on Phyllotreta striolata, indoor toxicity tests of different proportions of compound I and lotilaner on Phyllotreta striolata were carried out. The corresponding concentrations were prepared according to the mass proportions in the table, and indoor insecticidal tests were carried out. The test results are as follows:

[0202] Table 10 Toxicity test results of compound I and lotilaner and their mixtures on Phyllotreta striolata

[0203] active ingredient ratio regression equation LC 50 ]]> co-toxicity factor evaluation Compound I — y = 5.8432 + 1.4063x 0.2514 — — lotilaner — y = 3.0965 + 1.2515x 33.1921 — — Compound I: lotilaner 50:1 y = 5.8516 + 1.2209x 0.2007 127.7465 synergism Compound I: lotilaner 10:1 y = 5.9079 + 1.2825x 0.1959 141.0556 synergism Compound I: lotilaner 1:1 y = 5.9134 + 1.4716x 0.2395 208.3592 synergism Compound I: lotilaner 1:10 y = 4.6374 + 1.7118x 1.6287 157.8517 synergism Compound I: lotilaner 1:50 y = 3.8887 + 1.3321x 6.8269 136.2592 synergism

[0204] As shown in Table 10, the different proportions of compound I and lotilaner have obvious synergistic effect on Phyllotreta striolata.

[0205] Three, field efficacy examples

[0206] In order to clarify the control effect of compound I and GABA-gated chloride channel antagonist single agent and mixture on Plutella xylostella, Spodoptera exigua, Ostrinia nubilalis and Phyllotreta striolata, multiple efficacy tests were carried out in different regions of China. The preparations were processed according to Preparation Examples 1-7. For example, the preparation in Table 12 (other test preparations refer to the same preparation example), the specific preparation method is as follows:

[0207] Table 11 Preparation of compound I·bistrifluron suspension concentrate with different proportions

[0208]

[0209] Field efficacy test of Plutella xylostella and Spodoptera exigua

[0210] Test method: According to the application method specified in "National Standard of the People's Republic of China GB / T 17980.13-2000". The first application was carried out when there were enough number of larvae (1-3 three instar larvae per plant).

[0211] Investigation method: According to the investigation method stipulated in the "People's Republic of China National Standard GB / T 17980.13-2000", count the number of live larvae of different ages on each plot on at least 10 plants, and investigate the whole plant. Investigate the initial population before application, and investigate the number of live insects 1-3 days, 7-14 days after treatment. The control effect formula is:

[0212]

[0213]

[0214] Corn armyworm field efficacy test

[0215] Test method: According to the application method stipulated in the "People's Republic of China National Standard GB / T 17980.80-2004". Apply at the 2nd and 3rd instar larvae in the field.

[0216] Investigation method: According to the investigation method stipulated in the "People's Republic of China National Standard GB / T 17980.80-2004", sample 5 points per plot, and take 1m 2 Investigate the number of live insects on the crop. Investigate the initial population before application, and investigate once 1, 3, 7 days after application. The control effect formula is:

[0217]

[0218]

[0219] Cabbage striped flea beetle field efficacy test

[0220] Test method: According to the application method stipulated in the "People's Republic of China National Standard GB / T 17980.18-2000".

[0221] Investigation method: According to the investigation method stipulated in the "People's Republic of China National Standard GB / T 17980.18-2000", randomly select at least 20 plants per plot to investigate the number of live adult insects. Investigate the initial population before application, and investigate and record the number of live insects the day after and the seventh day after application, and calculate the control effect. The control effect formula is:

[0222]

[0223]

[0224] Table 12 Field efficacy test of compound I and bromide fluorobenzene bisamide on cabbage moth

[0225]

[0226]

[0227] Note: The same column data after different letters represent significant difference at the level of P <0.05 by Duncan's new multiple range test, the same below.

[0228] The 10% compound I·flubendiamide suspension (1:4) in Example 11 was prepared according to Reference Preparation Example 3, wherein 10% refers to the total mass percentage of active substances (compound I and flubendiamide), and 1:4 refers to the mass ratio of the two active ingredients, compound I and flubendiamide.

[0229] The 10% compound I·flubendiamide suspension (1:3) in Example 12 was prepared according to Reference Preparation Example 3, wherein 10% refers to the total mass percentage of active substances (compound I and flubendiamide), and 1:3 refers to the mass ratio of the two active ingredients, compound I and flubendiamide.

[0230] The 10% compound I suspension in Comparative Example 1 was also prepared according to Reference Preparation Example 3, wherein 10% refers to the mass percentage of active substance, compound I.

[0231] The following other examples and comparative examples are also prepared in this way, which will not be described in detail here.

[0232] As can be seen from the test results in the table, in the prevention and control of cabbage moths, the 1-day, 3-day and 7-day control effects of compound I and flubendiamide complex are significantly higher than those of single agent at the same active ingredient dosage (Comparative Examples 1 and 2 corresponding to Examples 11-17). The 7-day control effect can still reach more than 93.98%, with a long effective period.

[0233] Table 13 Field efficacy test of compound I and flubendiamide on cabbage striped flea beetle

[0234]

[0235]

[0236] As can be seen from the test results in the table, in the prevention and control of cabbage moths, the 1-day, 3-day and 7-day control effects of compound I and flubendiamide complex are significantly higher than those of single agent at the same active ingredient dosage (Comparative Examples 1 and 2 corresponding to Examples 11-17). The 7-day control effect can still reach more than 93.98%, with a long effective period.

[0237] Table 14 Field efficacy test of compound I and flubendiamide on cabbage striped flea beetle

[0238]

[0239] From the determination results, in the prevention and control of diamondback moth, compound I and cyflumetofen complex and single agent compared with the same effective component dosage (comparative example 5 and 6 corresponding to example 25-31), compound I and cyflumetofen complex on the 1, 3 and 7 days of diamondback moth control effect is obviously higher than that of single agent. Among them, the 7-day control effect can still reach more than 94.51%, the effective period is longer.

[0240] Table 15 Compound I and cyflumetofen complex on corn armyworm field efficacy test

[0241]

[0242]

[0243] From the determination results, in the prevention and control of diamondback moth, compound I and cyflumetofen complex and single agent compared with the same effective component dosage (comparative example 5 and 6 corresponding to example 25-31), compound I and cyflumetofen complex on the 1, 3 and 7 days of diamondback moth control effect is obviously higher than that of single agent. Among them, the 7-day control effect can still reach more than 94.51%, the effective period is longer.

[0244] Table 16 Compound I and cyflumetofen complex on cabbage armyworm field efficacy test

[0245]

[0246] From the determination results in the table, in the prevention and control of diamondback moth, compound I and cyflumetofen complex and single agent compared with the same effective component dosage (comparative example 9 and 10 corresponding to example 39-45), compound I and cyflumetofen complex on the 1 day, 3 days and 7 days of diamondback moth control effect is obviously higher than that of single agent. Among them, the 7-day control effect can still reach more than 95.42%, the effective period is longer.

[0247] Table 17 Compound I and cyflumetofen complex on corn armyworm field efficacy test

[0248]

[0249] From the determination results in the table, in the prevention and control of diamondback moth, compound I and cyflumetofen complex and single agent compared with the same effective component dosage (comparative example 9 and 10 corresponding to example 39-45), compound I and cyflumetofen complex on the 1 day, 3 days and 7 days of diamondback moth control effect is obviously higher than that of single agent. Among them, the 7-day control effect can still reach more than 95.42%, the effective period is longer.

[0250] Table 18 Field efficacy test of compound I and 1-(2,6-dichloro-a,a,a-trifluoro-p- tolyl)-4-(fluoromethylthio)-5-[(pyrazinylmethyl)amino]pyrazole-3-carbonitrile complex on Phyllotreta striolata

[0251]

[0252]

[0253] From the results in the table, in the prevention and treatment of Phyllotreta striolata, compound I and 1-(2,6-dichloro-a,a,a-trifluoro-p-tolyl)-4-(fluoromethylthio)-5-[(pyrazinylmethyl)amino]pyrazole-3-carbonitrile complex has a 2-day, 7-day control effect on Phyllotreta striolata significantly higher than that of a single agent at the same active ingredient dosage (comparative examples 13 and 14 corresponding to examples 53-59). The 7-day control effect can still reach more than 94.21%, and the duration is longer.

[0254] Table 19 Field efficacy test of compound I and 1-(2,6-dichloro-a,a,a-trifluoro-p- tolyl)-4-(fluoromethylthio)-5-[(pyrazinylmethyl)amino]pyrazole-3-carbonitrile complex on Spodoptera exigua

[0255]

[0256]

[0257] From the results in the table, in the prevention and treatment of Spodoptera exigua, compound I and 1-(2,6-dichloro-a,a,a-trifluoro-p-tolyl)-4-(fluoromethylthio)-5-[(pyrazinylmethyl)amino]pyrazole-3-carbonitrile complex has a 1-day, 3-day, and 7-day control effect on Spodoptera exigua significantly higher than that of a single agent at the same active ingredient dosage (comparative examples 15 and 16 corresponding to examples 60-66). The 7-day control effect can still reach more than 92.26%, and the duration is longer.

[0258] The above describes embodiments of the present application. However, the present application is not limited to the above-described embodiments. Any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present application should be included in the protection scope of the present application.

Claims

1. A composition containing compound I and a GABA-gated chloride channel antagonist, characterized in that, The GABA-gated chloride channel antagonist is selected from isoxazolamide; The structural formula of compound I is shown below. I The mass ratio of compound I to the GABA-gated chloride channel antagonist is 50~1:1~50.

2. The composition according to claim 1, characterized in that, The mass ratio of compound I to the GABA-gated chloride channel antagonist is 10~1:1~10.

3. The composition according to claim 1 or 2, characterized in that, Based on the total weight of the composition as 100%, the sum of the mass of compound I and the GABA-gated chloride channel antagonist in the composition is 1 to 80%.

4. The composition according to claim 3, characterized in that, The composition further includes at least one of the following: deionized water, organic solvent, emulsifier, dispersant, wetting agent, thickener, defoamer, stabilizer, binder, disintegrant, antifreeze, anticaking agent, suspending agent, film-forming agent, preservative, colorant, polymeric capsule wall material, pH adjuster, or filler.

5. Use of the composition according to any one of claims 1-4 for the prevention and control of pests in agriculture or non-agriculture.

6. The use according to claim 5, characterized in that, The pests mentioned are selected from Lepidoptera, Hemiptera, Coleoptera, Thysanoptera, Diptera, and Mite pests.

7. A method for controlling pests in agricultural or non-agricultural fields, characterized in that, This includes applying the composition of any one of claims 1-4 to plants infested with pests.

8. A composition for controlling invertebrate pests, characterized in that, It contains a biologically effective amount of compound I, a GABA-gated chloride channel antagonist, and at least one other component selected from surfactants, solid diluents, and liquid diluents, wherein the GABA-gated chloride channel antagonist is selected from isoxazolidinamide; The structural formula of compound I is shown below. I The mass ratio of compound I to the GABA-gated chloride channel antagonist is 50~1:1~50.

9. A method for controlling invertebrate pests, characterized in that, This includes contacting invertebrate pests or their environment with a biologically effective amount of the composition according to any one of claims 1-4.

10. A spray composition comprising the composition according to any one of claims 1-4 and a propellant.

11. A bait composition comprising the composition according to any one of claims 1-4 and one or more food materials.

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