A microfluidic luminescence detection device and method, electronic equipment and storage medium

Abstract

This invention relates to the field of sample detection technology, and more particularly to a microfluidic luminescence detection device, method, electronic device, and storage medium. The device includes: a housing, and a rotating assembly, a microfluidic chip, a detection assembly, and a control module disposed within the housing. The rotating assembly and the detection assembly are electrically connected to the control module, and the microfluidic chip is detachably mounted on the rotating assembly. The microfluidic chip has a channel group and a sample detection chamber. The channel group includes a pre-excitation liquid channel, an excitation liquid channel, a reconstitution solution channel, a cleaning liquid channel, a sample liquid channel, and a waste liquid channel. Each channel in the channel group includes one or more chambers, and the chambers and the sample detection chamber are connected through microfluidic channels. The detection assembly includes a photosensitive sensor assembly and a light-emitting element. This application can improve the adaptability of the microfluidic luminescence detection device to different detection items, enhance its adaptability to differentiated sample detection and analysis scenarios, and reduce the cost of differentiated adaptation of the microfluidic chip.

Description

Technical Field

[0001] This invention relates to the field of sample detection technology, and in particular to a microfluidic luminescence detection device, method, electronic device, and storage medium. Background Technology

[0002] Chemiluminescence analyzers are used to analyze liquid samples such as blood. Traditional analytical devices are mostly tubular analyzers, which require adding blood samples to test tubes, centrifuging the blood to separate serum and plasma before they can be analyzed by the instrument. In this process, the reaction carriers in tubular chemiluminescence analyzers are reaction tubes or reaction cups, which usually need to be transferred in the equipment along with the process steps. The mechanical structure involved in the automatic feeding of reaction tubes or reaction cups, adding samples, adding reagents, adding substrates, photon detection, waste recovery, and needle washing is complex and bulky. Therefore, the more convenient and efficient microfluidic chip detection technology has begun to be favored.

[0003] Microfluidic chip detection is commonly used in in vitro diagnostic products. It features small sample volume, high functional integration, disposable microfluidic chips, and portable and compact reader devices, making it particularly suitable for rapid on-site testing applications. The detection principle involves placing a sample in a microfluidic chip and then allowing the sample fluid in the microfluidic chip to flow at a predetermined speed and direction to achieve functions such as quantitative sample distribution, contact and mixing of sample and reagent components.

[0004] In existing microfluidic analysis devices, a microfluidic chip is driven by centrifugal force. The microfluidic chip is disk-shaped with a central mounting hole. During operation, this hole is mounted on the analyzer's motor shaft, allowing it to rotate at high speed along with the motor shaft. Under the influence of centrifugal force, the sample flows from the inner to the outer ring of the disk. Multiple interconnected reaction chambers are also arranged circumferentially outwards from the microfluidic chip. The sample enters each chamber sequentially under centrifugal force for reaction and then is used for detection. By controlling the speed and direction of rotation, the movement / stopping or oscillating mixing of the fluid can be achieved. This demonstrates a significant advantage over conventional methods. In the tube-liquid fluid control method, the microfluidic chip manufacturing process is relatively complex. Therefore, the flow channel design concept is generally that a single flow channel serves a single detection item. For example, Chinese patent CN103323605B provides a microfluidic chip for immunoassay of glycated hemoglobin in serum, which includes multiple interconnected and centrally symmetrically distributed flow channels. Although it can detect multiple samples simultaneously, each flow channel completes the detection operation independently. Therefore, the same microfluidic chip can only perform detection of the same type or the same physicochemical properties, and is not suitable for complex detection items.

[0005] It is evident that existing microfluidic analysis equipment has the following drawbacks: the flow channel setup of microfluidic chips is limited to a single method, making it difficult to apply to complex detection projects such as multi-stage luminescence detection; the same microfluidic chip can only perform detection of the same type of project, failing to meet multiple detection needs simultaneously, and has weak adaptability to scenarios involving the detection and analysis of differentiated samples. Summary of the Invention

[0006] In view of the shortcomings of the prior art, the purpose of the present invention is to provide a microfluidic luminescence detection device, method, electronic device and storage medium to solve the problems of the existing microfluidic chip having a single flow channel setting method, which is difficult to apply to complex detection projects such as multi-process luminescence detection, the same microfluidic chip can only perform detection of the same type of project, and cannot meet multiple detection needs at the same time, and has weak adaptability to different sample detection and analysis scenarios.

[0007] To achieve the above and other related objectives, in a first aspect, this application provides a microfluidic luminescence detection device, the device comprising:

[0008] The housing, and a rotating component, a microfluidic chip, a detection component, and a control module disposed within the housing, wherein the rotating component and the detection component are electrically connected to the control module, and the microfluidic chip is detachably mounted on the rotating component;

[0009] The microfluidic chip is provided with a flow channel group and a sample detection chamber. The flow channel group includes a pre-excitation liquid flow channel, an excitation liquid flow channel, a rehydration solution flow channel, a cleaning liquid flow channel, a sample liquid flow channel, and a waste liquid flow channel. Each flow channel in the flow channel group includes one or more chambers. Each chamber and the sample detection chamber are connected by a microfluidic channel. The pre-excitation liquid flow channel, the excitation liquid flow channel, the rehydration solution flow channel, the cleaning liquid flow channel, and the sample liquid flow channel are connected in parallel and converge at the inlet side of the sample detection chamber. The waste liquid flow channel is connected to the outlet side of the sample detection chamber.

[0010] The detection assembly includes a photosensitive component and a light-emitting element. The photosensitive component is respectively disposed in relation to at least one of the pre-excitation liquid channel, the excitation liquid channel, the reconstitution liquid channel, the cleaning liquid channel, the sample liquid channel, and the waste liquid channel, as well as the sample detection cavity. The light-emitting element is disposed in a rotation path toward the sample detection cavity.

[0011] Furthermore, the light-emitting element includes a PMT optical path system, which includes multiple photomultiplier tubes, with at least two of the photomultiplier tubes facing the same sample detection cavity at the same position on its rotation path, for synchronously acquiring the light emission state from the same position.

[0012] Furthermore, on the rotation path of the same sample detection cavity, the angle between the light receiving directions of any two photomultiplier tubes facing the same position is less than or equal to 30°, and the light receiving ends of any two photomultiplier tubes facing the same position are provided with anti-reflection coatings of different corresponding wavelengths, the anti-reflection coatings being used to filter the received light of the corresponding wavelengths.

[0013] Furthermore, the pre-excitation liquid flow channel includes a pre-excitation liquid storage chamber, a pre-excitation liquid metering chamber, and a pre-excitation liquid quality control chamber. The pre-excitation liquid storage chamber, the pre-excitation liquid metering chamber, and the sample detection chamber are connected in series. The pre-excitation liquid quality control chamber is connected to the pre-excitation liquid metering chamber. The optical sensor assembly includes a first optical sensor disposed along the rotation path of the pre-excitation liquid quality control chamber.

[0014] Furthermore, the excitation fluid channel includes an excitation fluid storage chamber connected to the sample detection chamber.

[0015] Furthermore, the reconstitution channel includes a reconstitution storage chamber, a reconstitution quantification chamber, and a reconstitution quality control chamber, wherein the reconstitution storage chamber, the reconstitution quantification chamber, and the sample detection chamber are connected in series, and the reconstitution quality control chamber is connected to the reconstitution quantification chamber. The optical sensor assembly includes a second optical sensor disposed along a rotation path toward the reconstitution quality control chamber.

[0016] Furthermore, the cleaning fluid flow channel includes a cleaning fluid storage chamber, a cleaning fluid metering chamber, and a cleaning fluid quality control chamber. The cleaning fluid storage chamber, the cleaning fluid metering chamber, and the sample detection chamber are connected in series. The cleaning fluid quality control chamber is connected to the cleaning fluid metering chamber. The optical sensor assembly includes a third optical sensor disposed along the rotation path of the cleaning fluid quality control chamber.

[0017] Furthermore, multiple cleaning fluid storage chambers are arranged in series, and multiple cleaning fluid quality control chambers are arranged in series, with the number of cleaning fluid storage chambers being the same as the number of cleaning fluid quality control chambers.

[0018] Furthermore, the sample liquid flow channel includes a plasma receiving chamber, a first plasma separation chamber, a second plasma separation chamber, and a plasma quality control chamber. The plasma receiving chamber, the first plasma separation chamber, and the second plasma separation chamber are connected in series. The sample detection chamber is connected in series between the first plasma separation chamber and the second plasma separation chamber. The plasma quality control chamber is connected to the first plasma separation chamber. The optical sensor assembly includes a fourth optical sensor disposed along the rotation path of the cleaning liquid quality control chamber.

[0019] Furthermore, the detection assembly also includes a robotic arm disposed inside the housing and a second drive component connected to the robotic arm. The second drive component is used to drive the robotic arm to move and is electrically connected to the control module. At least some of the detection elements of the detection assembly are disposed on the robotic arm.

[0020] Furthermore, an interactive panel is embedded in the housing, which is electrically connected to the control module and is used to input control signals to the control module and / or to output output signals from the control module.

[0021] Furthermore, it also includes a printing device disposed within the housing, the printing device being electrically connected to the control module for receiving output information from the control module and printing.

[0022] Secondly, this application provides a microfluidic luminescence detection method, which uses the microfluidic luminescence detection device described above to perform microfluidic luminescence detection. The microfluidic luminescence detection method includes:

[0023] S1, a microfluidic chip is provided, wherein the pre-excitation liquid channel, the excitation liquid channel, the reconstitution solution channel and the cleaning liquid channel of the microfluidic chip are respectively filled with pre-excitation liquid, excitation liquid, reconstitution solution and cleaning liquid, and the sample detection chamber is provided with a reagent to be reacted, wherein the reagent to be reacted is a lyophilized reagent;

[0024] S2, after adding the sample to be tested to the microfluidic chip, the sample liquid channel is injected with the sample to be tested, and then the microfluidic chip is installed on the rotating assembly;

[0025] S3, based on the first rotation speed parameter, the rotating component is controlled to rotate, so that the reconstituted solution is quantitatively released into the sample detection chamber and mixed with the reagent to be reacted for incubation;

[0026] S4, the rotating component is controlled to rotate based on the second rotation speed parameter so that the plasma is quantitatively released into the sample detection chamber;

[0027] S5, the rotating component is controlled to rotate based on the third rotation speed parameter so that the cleaning solution is quantitatively released into the sample detection chamber;

[0028] S6, the rotating component is controlled to rotate based on the fourth rotation speed parameter so that the mixed liquid in the sample detection chamber is discharged into the waste liquid channel;

[0029] S7, the rotating component is controlled to rotate based on the fifth rotation speed parameter so that the pre-excitation liquid is quantitatively released into the sample detection chamber and mixed with the reagent to be reacted for incubation;

[0030] S8, the rotating component is controlled to rotate based on the sixth rotation speed parameter, so that the excitation liquid is quantitatively released into the sample detection chamber to generate a light signal;

[0031] S9, the detection component detects the optical signal and transmits it to the control module in a preset format, whereby the control module analyzes and processes the optical signal.

[0032] Thirdly, this application provides an electronic device, comprising:

[0033] One or more processors;

[0034] A storage device for storing one or more programs, which, when executed by one or more processors, enable the electronic device to implement the microfluidic luminescence detection method as described above.

[0035] Fourthly, this application provides a computer-readable storage medium having a computer program stored thereon, which, when executed by a processor, implements the microfluidic luminescence detection method as described above.

[0036] The microfluidic luminescence detection device, method, electronic device, and storage medium described above, by setting a flow channel group and a sample detection chamber on a microfluidic chip, wherein the flow channel group includes a pre-excitation liquid flow channel, an excitation liquid flow channel, a rehydration solution flow channel, a cleaning liquid flow channel, a sample liquid flow channel, and a waste liquid flow channel, and the chambers in each flow channel are connected by microfluidic channels, so that the flow control of liquid in different chambers can be completed at different rotation speeds. Furthermore, the flow channel group includes at least one of the pre-excitation liquid flow channel, the excitation liquid flow channel, the rehydration solution flow channel, the cleaning liquid flow channel, the sample liquid flow channel, and the waste liquid flow channel, as well as the sample detection chamber. Optical sensor components are installed at various locations to acquire and control the liquid state in different chambers of each flow channel in real time. This allows for the monitoring of the start of various analytical steps in conjunction with the flow channel configuration. Furthermore, by arranging and combining fluid control schemes in multiple channels and chambers, various complex detection items such as luminescence detection can be completed. This effectively meets the requirements of different detection steps and items, significantly improves the adaptability of the microfluidic luminescence detection device to different detection items, greatly enhances its adaptability to the detection and analysis of differentiated samples, and reduces the cost of differentiated adaptation of microfluidic chips. Attached Figure Description

[0037] Figure 1 This is a schematic diagram of the axial structure of a microfluidic luminescence detection device shown in an exemplary embodiment of this application;

[0038] Figure 2 This is a schematic diagram of the structure of a microfluidic luminescence detection device after the top cover is opened, as shown in an exemplary embodiment of this application.

[0039] Figure 3This is a schematic diagram of the internal structure of a microfluidic luminescence detection device shown from a rear-side view, illustrating an exemplary embodiment of this application.

[0040] Figure 4 This is a partial enlarged structural diagram of the internal structure of a microfluidic luminescence detection device shown in an exemplary embodiment of this application;

[0041] Figure 5 A schematic diagram of the overall structure of a microfluidic chip shown in an exemplary embodiment of this application.

[0042] Figure 6 This is a schematic diagram illustrating the flow channel division within a semi-circular region of a microfluidic chip, as shown in an exemplary embodiment of this application.

[0043] Figure 7 This is a schematic flowchart illustrating a microfluidic luminescence detection method as an exemplary embodiment of this application;

[0044] Figure 8 This is a schematic diagram of the structure of a computer system suitable for implementing the electronic device of the present application.

[0045] Part Number Explanation

[0046] 1-Shell; 11-Top cover;

[0047] 2-Rotating assembly; 21-First driving component

[0048] 3-Microfluidic chip; 30-Sample detection chamber; 300-Lyophilized reagent sphere; 301-Sample detection area; 302-Calibration detection area; 31-Pre-excitation fluid channel; 311-Pre-excitation fluid storage chamber; 312-Pre-excitation fluid metering chamber; 313-Pre-excitation fluid quality control chamber; 32-Excitation fluid channel; 321-Excitation fluid storage chamber; 33-Reconstitution solution channel; 331-Reconstitution solution storage chamber; 332-Reconstitution solution metering chamber; 333-Reconstitution solution quality control chamber; 34-Washing fluid channel; 341-Washing fluid storage chamber; 342-Washing fluid metering chamber; 343-Washing fluid quality control chamber; 35-Sample liquid channel; 351-Plasma container chamber; 352-Plasma first separation chamber; 353-Plasma second separation chamber; 354-Plasma quality control chamber; 36-Waste liquid channel; 37-Calibration detection chamber;

[0049] 4-Detection component; 411-First optical sensor; 412-Second optical sensor; 413-Third optical sensor; 414-Fourth optical sensor; 42-Light-emitting element; 43-Robotic arm; 44-Second drive component;

[0050] 5-Control panel;

[0051] 6-Interactive panel;

[0052] 7-Printing device. Detailed Implementation

[0053] The following specific embodiments illustrate the implementation of the present invention. Those skilled in the art can easily understand other advantages and effects of the present invention from the content disclosed in this specification.

[0054] It should be understood that the structures, proportions, sizes, etc., illustrated in the accompanying drawings of this specification are merely for illustrative purposes to aid those skilled in the art and are not intended to limit the scope of the invention. Therefore, they have no substantial technical significance. Any modifications to the structure, changes in proportions, or adjustments to size, without affecting the effectiveness and purpose of the invention, should still fall within the scope of the technical content disclosed in this invention. Furthermore, the terms such as "upper," "lower," "left," "right," "middle," "first," and "second" used in this specification are merely for clarity and are not intended to limit the scope of the invention. Changes or adjustments to their relative relationships, without substantially altering the technical content, should also be considered within the scope of the invention.

[0055] In one embodiment, this application exemplarily illustrates a microfluidic luminescence detection device; please refer to [link to relevant documentation]. Figures 1-6 The microfluidic light emission detection device includes at least a housing 1, a rotating component 2, a microfluidic chip 3, a detection component 4, and a control module disposed within the housing 1.

[0056] In this embodiment, the housing 1 includes an openable and closable top cover 11 disposed above the rotating assembly 2. In other embodiments, the housing 1 may also be provided with other structures such as openings and covers for the microfluidic chip 3 to enter and exit the housing 1 and to be installed and removed from the rotating assembly 2.

[0057] The rotating component 2 and the detection component 4 are electrically connected to the control module. It can be understood that the flow control of the liquid in the internal channel of the microfluidic chip 3 relies on the centrifugal force generated when the microfluidic chip 3 rotates. The rotating component 2 is the component used to drive the microfluidic chip 3 to rotate and generate centrifugal force. Therefore, during detection, the microfluidic chip 3 is detachably mounted on the rotating component 2. The rotating component 2 includes at least one rotatable first driving component 21, such as but not limited to a motor, and a mounting structure that rotates under the drive of the first driving component 21 and is detachably connected to the microfluidic chip 3. The detachable connection here includes but is not limited to snap-fit ​​connection, magnetic element magnetic connection, and other connection methods. When the microfluidic chip 3 is mounted on the rotating component 2, the rotating component 2 drives the microfluidic chip 3 to rotate.

[0058] The microfluidic chip 3 is equipped with a flow channel group and a sample detection chamber 30. The flow channel group includes a pre-excitation liquid flow channel 31, an excitation liquid flow channel 32, a rehydration solution flow channel 33, a cleaning liquid flow channel 34, a sample liquid flow channel 35, and a waste liquid flow channel 36. Each flow channel in the flow channel group includes one or more chambers. The chambers and the sample detection chamber 30 are connected by microfluidic channels. The pre-excitation liquid flow channel 31, the excitation liquid flow channel 32, the rehydration solution flow channel 33, the cleaning liquid flow channel 34, and the sample liquid flow channel 35 are connected in parallel and converge into the sample detection chamber. On the inlet side of 30, the waste liquid flow channel 36 is connected to the outlet side of the sample detection chamber 30. It is worth noting that a microchannel refers to a fluid flow path with micrometers as the unit. Depending on the properties of the fluid itself, different microchannel sizes and different rotation speeds will affect the fluid's permeability within the microchannel. Thus, based on different preset rotation speeds, the flow control of fluids in different channels on the same microfluidic chip 3 can be achieved. Furthermore, according to the preset rotation speed arrangement, the fluid flow actions required for different detection procedures can be executed.

[0059] The detection component 4 includes a photosensitive component and a light-emitting element 42. The photosensitive component is respectively positioned facing at least one of the pre-excitation liquid channel 31, the excitation liquid channel 32, the reconstitution liquid channel 33, the cleaning liquid channel 34, the sample liquid channel 35, and the waste liquid channel 36, as well as the sample detection chamber 30. The light-emitting element 42 is positioned facing the sample detection chamber 30. It should be understood that the sensor component has at least two functions: first, the light-emitting element 42 and the photosensitive component work together to detect the sample in the sample detection chamber 30; second, it monitors the liquid state in each chamber so as to understand the execution status of the detection steps corresponding to the current liquid state.

[0060] In the above embodiments, the control module includes, but is not limited to, programmable logic controllers (PLCs), central processing units (CPUs), and other components that have specific data receiving and processing functions based on preset programs and computer software, or circuit boards, devices, equipment, servers, or cloud processors that are equipped with / integrated with the above components.

[0061] As can be seen, in the microfluidic luminescence detection device provided in this application, by setting a flow channel group and a sample detection chamber 30 on the microfluidic chip 3, wherein the flow channel group includes a pre-excitation liquid flow channel 31, an excitation liquid flow channel 32, a rehydration solution flow channel 33, a cleaning liquid flow channel 34, a sample liquid flow channel 35, and a waste liquid flow channel 36, and the chambers in each flow channel are connected by microfluidics, so that the flow control of liquid in different chambers can be completed at different rotation speeds. Furthermore, at least one of the pre-excitation liquid flow channel 31, the excitation liquid flow channel 32, the rehydration solution flow channel 33, the cleaning liquid flow channel 34, the sample liquid flow channel 35, and the waste liquid flow channel 36 is directed towards the flow channel 36. Optical sensor components are respectively set at the positions of the sample detection chamber 30 to acquire and control the liquid state of different chambers in each flow channel in real time. It can be used in conjunction with the flow channel setting to complete the initial monitoring of various analysis steps. Furthermore, by arranging and combining fluid control schemes in multiple flow channels and multiple chambers, it can complete the detection of various complex detection items such as luminescence detection, effectively meeting the needs of different detection steps and detection items, effectively improving the adaptability of the microfluidic luminescence detection device to different detection items, greatly improving the adaptability to the detection and analysis of differentiated samples, and reducing the differentiated adaptation cost of the microfluidic chip 3.

[0062] In this embodiment, please refer to Figure 5 The microfluidic chip 3 is a disk-shaped structure formed by splicing two semi-circular regions. The flow channel group is set as two centrally symmetrical groups along the circumference of the disk-shaped microfluidic chip 3, located in the two semi-circular regions respectively. In some other embodiments, the flow channel group can also be set as three or more groups on the same disk according to its size and design requirements, so as to perform detection of multiple groups of samples at the same time.

[0063] In this embodiment, please refer to Figure 6 The microfluidic chip 3 is divided into a sample detection area 301 and a calibration detection area 302 within each channel group. In each channel group, except for the sample liquid channel 35, each channel is provided with two sets, one set located in the sample detection area 301 and the other in the calibration detection area 302. The sample detection chamber 30 is located in the sample detection area 301. The calibration detection area 302 is also provided with a calibration detection chamber 37. The calibration detection chamber 37 is used to place the same reagent as in the sample detection area 301, such as lyophilized reagent balls 300, but no sample liquid enters the calibration detection area 302. It can be understood that the calibration detection area 302 performs detection synchronously with the sample detection area 301, and its detection results are not involved by sample liquid. Therefore, its detection results are used as a control group or for calibration.

[0064] Furthermore, in some other embodiments, in order to facilitate the acquisition of fluid flow conditions in each channel, quantitative chambers and quality control chambers can be connected at other locations in each channel. For example, in this embodiment, quantitative chambers and quality control chambers are also connected at the inlet ends of the sample detection chamber 30 and the calibration detection chamber 37, and additional quality control chambers are connected on the sample liquid channel 35 and the waste liquid channel 36.

[0065] In this embodiment, the light-emitting element 42 includes a PMT optical path system, which includes multiple photomultiplier tubes. At least two photomultiplier tubes are arranged facing the same sample detection cavity 30 at the same position on its rotation path. That is, at least two photomultiplier tubes face the same point, which is any point on the same sample detection cavity 30 on its rotation path. The at least two photomultiplier tubes arranged in this way are used to synchronously acquire the light emission state of the same sample detection cavity 30 from the same position.

[0066] In this embodiment, on the rotation path of the same sample detection cavity 30, the angle between the light receiving directions of any two photomultiplier tubes facing the same position is less than or equal to 30° to avoid light intensity errors due to different emission angles. Furthermore, on the rotation path of the same sample detection cavity 30, the light receiving ends of any two photomultiplier tubes facing the same sample detection cavity 30 are provided with anti-reflective coatings of different corresponding wavelengths. These anti-reflective coatings are used to filter the received light of corresponding wavelengths. By filtering the light signal of a specific wavelength received by the photomultiplier tubes through the anti-reflective coatings, different photomultiplier tubes can obtain the luminous intensity of the sample detection cavity 30 at different wavelengths. This allows for the detection of different samples within the sample detection cavity 30, and more accurate identification of the luminous emission of the same sample within different wavelength ranges. This avoids detection errors caused by mixed light, further improving the compatibility of the microfluidic luminescence detection device with different detection processes and enhancing the detection accuracy of the entire luminescence detection process.

[0067] Based on the above implementation, in some other embodiments, gradient division can be made based on the natural light spectrum, and a photomultiplier tube with an anti-reflection coating can be set for each gradient range. Based on the luminescence obtained under each gradient range, the control module can construct a spectral image or digital model corresponding to the luminescence of the sample detection cavity 30.

[0068] In this embodiment, the pre-excitation liquid flow channel 31 includes a pre-excitation liquid storage chamber 311, a pre-excitation liquid metering chamber 312, and a pre-excitation liquid quality control chamber 313. The pre-excitation liquid storage chamber 311 stores the pre-excitation liquid. The pre-excitation liquid storage chamber 311, the pre-excitation liquid metering chamber 312, and the sample detection chamber 30 are connected in series. The pre-excitation liquid quality control chamber 313 is connected to the pre-excitation liquid metering chamber 312. At a specific rotation speed, the pre-excitation liquid enters the pre-excitation liquid metering chamber 312 and the pre-excitation liquid quality control chamber 313 from the pre-excitation liquid storage chamber 311 through a microchannel. The pre-excitation liquid metering chamber 312 is used to measure the pre-excitation liquid in a single detection process. The required pre-excitation liquid is quantitatively stored. The optical sensor assembly includes a first optical sensor 411 positioned along the rotation path of the pre-excitation liquid quality control cavity 313. As the pre-excitation liquid quality control cavity 313 rotates with the microfluidic chip 3, at least one position on its rotation path is detected by the first optical sensor 411. Based on the detection result of the pre-excitation liquid storage state in the pre-excitation liquid quality control cavity 313 by the first optical sensor 411, the control module can understand the current flow state of the pre-excitation liquid. Specifically, the pre-excitation liquid enters the sample detection cavity 30 from the pre-excitation liquid storage cavity 311 in the following steps:

[0069] The microfluidic chip 3 rotates at a first preset speed, and the pre-excitation liquid enters the pre-excitation liquid metering chamber 312. After the pre-excitation liquid metering chamber 312 is full, it continues to rotate, and the pre-excitation liquid enters the pre-excitation liquid quality control chamber 313.

[0070] The first optical sensor 411 detects the liquid state of the pre-excitation liquid quality control chamber 313. After the control module learns that the pre-excitation liquid quality control chamber 313 is full, the microfluidic chip 3 rotates at a second preset speed, and the pre-excitation liquid enters the sample detection chamber 30 from the pre-excitation liquid quantitative chamber 312 and mixes with the detection sample for pre-excitation.

[0071] As can be seen, through the above structure, in conjunction with the rotating component 2 and the control module, the fluid flow state in the pre-excitation liquid channel 31 can be precisely controlled to meet the needs of different detection processes. It is understood that in other channels and chambers, such as the rehydration solution channel 33 and the cleaning solution channel 34, the fluid flow state can be precisely controlled through the same action logic.

[0072] In this embodiment, the excitation fluid channel 32 includes an excitation fluid storage cavity 321 connected to the sample detection cavity 30. The excitation fluid storage cavity 321 is used to store excitation fluid so as to excite different samples in the sample detection cavity 30 and generate different luminescence characteristics so that the photosensitive component can collect different detection information.

[0073] In this embodiment, the reconstitution channel 33 includes a reconstitution storage chamber 331, a reconstitution quantitative chamber 332, and a reconstitution quality control chamber 333. The reconstitution storage chamber 331, the reconstitution quantitative chamber 332, and the sample detection chamber 30 are connected in series. The reconstitution quality control chamber 333 is connected to the reconstitution quantitative chamber 332. The optical sensor assembly includes a second optical sensor 412 disposed along a rotation path toward the reconstitution quality control chamber 333.

[0074] In this embodiment, the cleaning fluid channel 34 includes a cleaning fluid storage chamber 341, a cleaning fluid metering chamber 342, and a cleaning fluid quality control chamber 343. The cleaning fluid storage chamber 341, the cleaning fluid metering chamber 342, and the sample detection chamber 30 are connected in series. The cleaning fluid quality control chamber 343 is connected to the cleaning fluid metering chamber 342. The optical sensor assembly includes a third optical sensor 413 disposed along the rotation path toward the cleaning fluid quality control chamber 343.

[0075] In this embodiment, multiple cleaning fluid storage chambers 341 are arranged in series, and multiple cleaning fluid quality control chambers 343 are arranged in series. The number of cleaning fluid storage chambers 341 is the same as the number of cleaning fluid quality control chambers 343. Multiple cleaning fluid quality control chambers 343 and cleaning fluid storage chambers 341 can meet the needs of different cleaning fluid volumes and different cleaning fluid release sequences in different detection processes. In some embodiments, to ensure accuracy, the number of third optical sensors 413 can also be set one-to-one with the number of cleaning fluid quality control chambers 343.

[0076] In this embodiment, the sample liquid flow channel 35 includes a plasma receiving chamber 351, a first plasma separation chamber 352, a second plasma separation chamber 353, and a plasma quality control chamber 354. The plasma receiving chamber 351, the first plasma separation chamber 352, and the second plasma separation chamber 353 are connected in series. The sample detection chamber 30 is connected in series between the first plasma separation chamber 352 and the second plasma separation chamber 353. The plasma quality control chamber 354 is connected to the first plasma separation chamber 352. The optical sensor assembly includes a fourth optical sensor 414 disposed along the rotation path toward the cleaning liquid quality control chamber 343.

[0077] The first plasma separation chamber 352 and the second plasma separation chamber 353 are used to centrifuge the plasma during the rotation of the microfluidic chip 3, so that some components are separated into the second plasma separation chamber 353, thereby meeting the requirements for plasma component separation during the luminescence detection process.

[0078] In the above embodiments, the control methods of the reconstitution fluid channel 33, the cleaning fluid channel 34, and the sample liquid channel 35 can all be the same as those of the excitation fluid channel 32. Through the cooperation of the sensor assembly, the control module, and the rotating assembly 2, the flow of fluid in each channel can be precisely controlled based on different preset rotation speeds.

[0079] In this embodiment, please refer to Figure 2 and Figure 4 The detection component 4 also includes a robotic arm 43 disposed inside the housing 1 and a second drive component 44 connected to the robotic arm 43. The second drive component 44 is used to drive the robotic arm 43 to move. The second drive component 44 is electrically connected to the control module. At least some of the detection elements of the detection component 4 are disposed on the robotic arm 43. It is understood that the detection elements here include, but are not limited to, one or more of the aforementioned sensor components and light-emitting elements 42. For example, in this embodiment, the first light sensor 411, the second light sensor 412, and the third light sensor 413 are directly fixedly installed inside the housing 1, and the fourth light sensor 414 and the light-emitting element 42 are installed on the robotic arm 43.

[0080] In this embodiment, please refer to Figures 1-3 An interactive panel 6 is embedded in the housing 1. The interactive panel 6 is electrically connected to the control module and is used to input control signals to the control module and / or to output output signals to the control module.

[0081] In this embodiment, please refer to Figure 3 The control module includes, for example, a control board 5 equipped with a CPU, and the control board 5 is provided with input and output ports for electrical connection with other components.

[0082] In this embodiment, please refer to Figures 1-3 The microfluidic light emission detection device also includes a printing device 7 disposed inside the housing 1. The printing device 7 is electrically connected to the control module and is used to receive the output information of the control module and print.

[0083] In one embodiment, this application also exemplarily illustrates a microfluidic luminescence detection method; please refer to [link to relevant documentation]. Figure 7 The method uses the microfluidic luminescence detection device in the aforementioned embodiments to perform microfluidic luminescence detection. The microfluidic luminescence detection method specifically includes the following steps:

[0084] S1 provides a microfluidic chip. In the microfluidic chip, the pre-excitation liquid channel, the excitation liquid channel, the reconstitution solution channel and the cleaning liquid channel are respectively filled with pre-excitation liquid, excitation liquid, reconstitution solution and cleaning liquid. The sample detection chamber is filled with the reagent to be reacted, which is a spherical lyophilized reagent.

[0085] S2, After adding the sample to be tested to the microfluidic chip, the sample liquid channel is injected with the sample to be tested, and then the microfluidic chip is installed on the rotating assembly.

[0086] S3, based on the first rotation speed parameter, the rotating component is controlled to rotate, so that the reconstituted solution is quantitatively released into the sample detection chamber and mixed with the reagent to be reacted for incubation;

[0087] S4, the rotating component is controlled to rotate based on the second rotation speed parameter so that plasma is quantitatively released into the sample detection chamber;

[0088] S5, the rotation of the rotating component is controlled based on the third rotation speed parameter so that the cleaning solution is quantitatively released into the sample detection chamber;

[0089] S6, based on the fourth rotation speed parameter, controls the rotation of the rotating component to discharge the mixed liquid in the sample detection chamber to the waste liquid channel;

[0090] S7, based on the fifth rotation speed parameter, controls the rotation of the rotating component to release the pre-excitation liquid quantitatively into the sample detection chamber to mix and incubate with the reagent to be reacted;

[0091] S8 controls the rotation of the rotating component based on the sixth rotation speed parameter, so that the excitation liquid is quantitatively released into the sample detection chamber to generate a light signal.

[0092] S9, the detection component detects the optical signal and transmits it to the control module in a preset format, whereby the control module analyzes and processes the optical signal.

[0093] For step S1, the lyophilized reagent may be, for example, the lyophilized reagent ball 300 in the foregoing embodiments.

[0094] For steps S3-S8, it should be understood that, depending on the different ways in which the number of chambers in each flow channel is set, any one of the first to sixth rotation speed parameters can be a numerical group composed of one or more preset rotation speed values, so as to accurately control the fluid flow in the flow channel. For example, the fluid control scheme in the pre-excited liquid flow channel shown in the previous embodiment can be regarded as a specific implementation of step S7 of this embodiment, wherein:

[0095] The pre-excitation liquid flow channel includes a pre-excitation liquid storage chamber, a pre-excitation liquid metering chamber, and a pre-excitation liquid quality control chamber. The pre-excitation liquid storage chamber stores the pre-excitation liquid. The pre-excitation liquid storage chamber, the pre-excitation liquid metering chamber, and the sample detection chamber are connected in series. The pre-excitation liquid quality control chamber is connected to the pre-excitation liquid metering chamber. At a specific rotation speed, the pre-excitation liquid enters the pre-excitation liquid metering chamber and the pre-excitation liquid quality control chamber from the pre-excitation liquid storage chamber through a microchannel. The pre-excitation liquid metering chamber is used to quantitatively store the pre-excitation liquid required for one detection process. The optical sensor assembly includes a first optical sensor facing the pre-excitation liquid quality control chamber. The control module detects the liquid state of the pre-excitation liquid in the pre-excitation liquid quality control chamber based on the detection result of the first optical sensor, and can understand the current flow state of the pre-excitation liquid. Specifically, the pre-excitation liquid enters the sample detection chamber from the pre-excitation liquid storage chamber in the following steps:

[0096] Step S7 specifically includes:

[0097] The microfluidic chip is controlled to rotate at a first preset speed, and the pre-excitation liquid enters the pre-excitation liquid metering chamber. After the pre-excitation liquid metering chamber is full, the chip is kept rotating, and the pre-excitation liquid enters the pre-excitation liquid quality control chamber.

[0098] The first optical sensor detects the liquid state of the pre-excitation liquid quality control chamber. After the control module learns that the pre-excitation liquid quality control chamber is full, the microfluidic chip rotates at the second preset speed, and the pre-excitation liquid enters the sample detection chamber from the pre-excitation liquid quantitative chamber and mixes with the detection sample for pre-excitation.

[0099] The first preset speed and the second preset speed together constitute the fifth rotational speed parameter.

[0100] It is understood that, according to the scheme shown in the above embodiments, the fluid flow in each channel can be precisely controlled based on the first to sixth rotational speed parameters.

[0101] As can be seen, in the microfluidic luminescence detection method provided in this application, by setting a flow channel group and a sample detection chamber on the microfluidic chip, wherein the flow channel group includes a pre-excitation liquid flow channel, an excitation liquid flow channel, a rehydration solution flow channel, a cleaning liquid flow channel, a sample liquid flow channel, and a waste liquid flow channel, and the chambers in each flow channel are connected by microchannels, so that the flow control of liquid in different chambers can be completed at different rotation speeds. Furthermore, the positions of at least one of the pre-excitation liquid flow channel, the excitation liquid flow channel, the rehydration solution flow channel, the cleaning liquid flow channel, the sample liquid flow channel, and the waste liquid flow channel, as well as the sample detection chamber, are... By incorporating optical sensor components, real-time acquisition and control of the liquid state in different chambers of each flow channel can be achieved. This enables the monitoring of the initiation of various analytical steps in conjunction with the flow channel configuration. Furthermore, through the arrangement and combination of fluid control schemes in multiple flow channels and chambers, various complex detection projects such as luminescence detection can be completed. This effectively meets the requirements of different detection steps and projects, significantly improves the adaptability of the microfluidic luminescence detection device to different detection projects, greatly enhances its adaptability to the detection and analysis of differentiated samples, and reduces the cost of differentiated adaptation of microfluidic chips.

[0102] An embodiment of this application also provides an electronic device, including: one or more processors; and a storage device for storing one or more programs, which, when executed by the one or more processors, enable the electronic device to implement the microfluidic luminescence detection method provided in the above embodiments.

[0103] Figure 8 A schematic diagram of a computer system suitable for implementing the embodiments of this application is shown. It should be noted that... Figure 8 The computer system 800 of the electronic device shown is merely an example and should not impose any limitation on the functionality and scope of use of the embodiments of this application.

[0104] like Figure 8As shown, the computer system 800 includes a Central Processing Unit (CPU) 801, which can perform various appropriate actions and processes based on programs stored in Read-Only Memory (ROM) 802 or programs loaded from storage portion 808 into Random Access Memory (RAM) 803, such as performing the methods described in the above embodiments. The RAM 803 also stores various programs and data required for system operation. The CPU 801, ROM 802, and RAM 803 are interconnected via a bus 804. An Input / Output (I / O) interface 805 is also connected to the bus 804.

[0105] The following components are connected to I / O interface 805: an input section 806 including a keyboard, mouse, etc.; an output section 807 including a cathode ray tube (CRT), liquid crystal display (LCD), etc., and speakers, etc.; a storage section 808 including a hard disk, etc.; and a communication section 809 including a network interface card such as a LAN (Local Area Network) card, modem, etc. The communication section 809 performs communication processing via a network such as the Internet. A drive 810 is also connected to I / O interface 805 as needed. A removable medium 811, such as a disk, optical disk, magneto-optical disk, semiconductor memory, etc., is installed on drive 810 as needed so that computer programs read from it can be installed into storage section 808 as needed.

[0106] Specifically, according to embodiments of this application, the processes described above with reference to the flowcharts can be implemented as computer software programs. For example, embodiments of this application include a computer program product comprising a computer program carried on a computer-readable medium, the computer program including a computer program for performing the methods shown in the flowcharts. In such embodiments, the computer program can be downloaded and installed from a network via communication section 809, and / or installed from removable medium 811. When the computer program is executed by central processing unit (CPU) 801, it performs various functions defined in the system of this application.

[0107] It should be noted that the computer-readable medium shown in the embodiments of this application can be a computer-readable signal medium or a computer-readable storage medium, or any combination of the two. A computer-readable storage medium can be, for example, an electrical, magnetic, optical, electromagnetic, infrared, or semiconductor system, apparatus, or device, or any combination thereof. More specific examples of a computer-readable storage medium may include, but are not limited to: an electrical connection having one or more wires, a portable computer disk, a hard disk, random access memory (RAM), read-only memory (ROM), erasable programmable read-only memory (EPROM), flash memory, optical fiber, portable compact disc read-only memory (CD-ROM), optical storage device, magnetic storage device, or any suitable combination thereof. In this application, a computer-readable signal medium may include a data signal propagated in baseband or as part of a carrier wave, carrying a computer-readable computer program. Such propagated data signals can take various forms, including but not limited to electromagnetic signals, optical signals, or any suitable combination thereof. Computer-readable signal media can also be any computer-readable medium other than computer-readable storage media, which can send, propagate, or transmit a program for use by or in connection with an instruction execution system, apparatus, or device. The computer program contained on the computer-readable medium can be transmitted using any suitable medium, including but not limited to wireless, wired, etc., or any suitable combination thereof.

[0108] The flowcharts and block diagrams in the accompanying drawings illustrate the architecture, functionality, and operation of possible implementations of systems, methods, and computer program products according to various embodiments of this application. Each block in a flowchart or block diagram may represent a module, segment, or portion of code, which contains one or more executable instructions for implementing a specified logical function. It should also be noted that in some alternative implementations, the functions indicated in the blocks may occur in a different order than those indicated in the drawings. For example, two consecutively indicated blocks may actually be executed substantially in parallel, and they may sometimes be executed in reverse order, depending on the functions involved. It should also be noted that each block in a block diagram or flowchart, and combinations of blocks in a block diagram or flowchart, can be implemented using a dedicated hardware-based system that performs the specified function or operation, or using a combination of dedicated hardware and computer instructions.

[0109] The units described in the embodiments of this application can be implemented in software or hardware, and the described units can also be located in a processor. The names of these units do not necessarily limit the specific unit itself.

[0110] Another aspect of this application provides a computer-readable storage medium storing a computer program thereon, which, when executed by a computer's processor, causes the computer to perform the microfluidic light emission detection method as described above. This computer-readable storage medium may be included in the electronic device described in the above embodiments, or it may exist independently and not assembled into the electronic device.

[0111] Another aspect of this application provides a computer program product or computer program including computer instructions stored in a computer-readable storage medium. A processor of a computer device reads the computer instructions from the computer-readable storage medium and executes the computer instructions, causing the computer device to perform the microfluidic luminescence detection method provided in the various embodiments described above.

[0112] The above embodiments are merely illustrative of the principles and effects of the present invention and are not intended to limit the invention. Any person skilled in the art can modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by those skilled in the art without departing from the spirit and technical concept disclosed in the present invention should still be covered by the claims of the present invention.

Claims

1. A microfluidic luminescence detection method, characterized in that, A microfluidic luminescence detection device, the device comprising: The housing, and a rotating component, a microfluidic chip, a detection component, and a control module disposed within the housing, wherein the rotating component and the detection component are electrically connected to the control module, and the microfluidic chip is detachably mounted on the rotating component; The microfluidic chip is provided with a flow channel group and a sample detection chamber. The flow channel group includes a pre-excitation liquid flow channel, an excitation liquid flow channel, a rehydration solution flow channel, a cleaning liquid flow channel, a sample liquid flow channel, and a waste liquid flow channel. Each flow channel in the flow channel group includes one or more chambers. Each chamber and the sample detection chamber are connected by a microfluidic channel. The pre-excitation liquid flow channel, the excitation liquid flow channel, the rehydration solution flow channel, the cleaning liquid flow channel, and the sample liquid flow channel are connected in parallel and converge at the inlet side of the sample detection chamber. The waste liquid flow channel is connected to the outlet side of the sample detection chamber. The detection assembly includes a photosensitive component and a light-emitting element. The photosensitive component is respectively disposed facing at least one of the pre-excitation liquid channel, the excitation liquid channel, the reconstitution liquid channel, the cleaning liquid channel, the sample liquid channel, and the waste liquid channel, as well as the sample detection chamber. The light-emitting element is disposed facing the sample detection chamber. Microfluidic luminescence detection methods include: S1, a microfluidic chip is provided, wherein the pre-excitation liquid channel, the excitation liquid channel, the reconstitution solution channel and the cleaning liquid channel of the microfluidic chip are respectively filled with pre-excitation liquid, excitation liquid, reconstitution solution and cleaning liquid, and the sample detection chamber is provided with a reagent to be reacted, wherein the reagent to be reacted is a lyophilized reagent; S2, after adding the sample to be tested to the microfluidic chip, the sample liquid channel is injected with the sample to be tested, and then the microfluidic chip is installed on the rotating assembly; S3, based on the first rotation speed parameter, the rotating component is controlled to rotate, so that the reconstituted solution is quantitatively released into the sample detection chamber and mixed with the reagent to be reacted for incubation; S4, the rotating component is controlled to rotate based on the second rotation speed parameter so that plasma is quantitatively released into the sample detection chamber; S5, the rotating component is controlled to rotate based on the third rotation speed parameter so that the cleaning solution is quantitatively released into the sample detection chamber; S6, the rotating component is controlled to rotate based on the fourth rotation speed parameter so that the mixed liquid in the sample detection chamber is discharged into the waste liquid channel; S7, the rotating component is controlled to rotate based on the fifth rotation speed parameter so that the pre-excitation liquid is quantitatively released into the sample detection chamber and mixed with the reagent to be reacted for incubation; S8, the rotating component is controlled to rotate based on the sixth rotation speed parameter so that the excitation liquid is quantitatively released into the sample detection chamber to generate a light signal; S9, the detection component detects the optical signal and transmits it to the control module in a preset format, whereby the control module analyzes and processes the optical signal.

2. The microfluidic luminescence detection method according to claim 1, characterized in that: The light-emitting element includes a PMT optical path system, which includes multiple photomultiplier tubes. At least two of the photomultiplier tubes are positioned at the same location on their rotation path, facing the same sample detection cavity, for synchronously acquiring the light emission state from the same location.

3. The microfluidic luminescence detection method according to claim 2, characterized in that: The angle between the light receiving directions of any two photomultiplier tubes facing the same position is less than or equal to 30°, and the light receiving ends of any two photomultiplier tubes facing the same position are provided with anti-reflection coatings of different corresponding wavelengths. The anti-reflection coatings are used to filter the received light of the corresponding wavelengths.

4. The microfluidic luminescence detection method according to claim 1, characterized in that: The pre-excitation liquid flow channel includes a pre-excitation liquid storage chamber, a pre-excitation liquid metering chamber, and a pre-excitation liquid quality control chamber. The pre-excitation liquid storage chamber, the pre-excitation liquid metering chamber, and the sample detection chamber are connected in series. The pre-excitation liquid quality control chamber is connected to the pre-excitation liquid metering chamber. The optical sensor assembly includes a first optical sensor arranged along the rotation path of the pre-excitation liquid quality control chamber.

5. The microfluidic luminescence detection method according to claim 1, characterized in that: The excitation fluid channel includes an excitation fluid storage chamber connected to the sample detection chamber.

6. The microfluidic luminescence detection method according to claim 1, characterized in that: The reconstitution flow channel includes a reconstitution storage chamber, a reconstitution quantification chamber, and a reconstitution quality control chamber. The reconstitution storage chamber, the reconstitution quantification chamber, and the sample detection chamber are connected in series. The reconstitution quality control chamber is connected to the reconstitution quantification chamber. The optical sensor assembly includes a second optical sensor disposed along the rotation path of the reconstitution quality control chamber.

7. The microfluidic luminescence detection method according to claim 1, characterized in that: The cleaning fluid flow channel includes a cleaning fluid storage chamber, a cleaning fluid metering chamber, and a cleaning fluid quality control chamber. The cleaning fluid storage chamber, the cleaning fluid metering chamber, and the sample detection chamber are connected in series. The cleaning fluid quality control chamber is connected to the cleaning fluid metering chamber. The optical sensor assembly includes a third optical sensor disposed along the rotation path of the cleaning fluid quality control chamber.

8. The microfluidic luminescence detection method according to claim 7, characterized in that: Multiple cleaning fluid storage chambers are arranged in series, and multiple cleaning fluid quality control chambers are arranged in series. The number of cleaning fluid storage chambers is the same as the number of cleaning fluid quality control chambers.

9. The microfluidic luminescence detection method according to claim 1, characterized in that: The sample liquid flow channel includes a plasma receiving chamber, a first plasma separation chamber, a second plasma separation chamber, and a plasma quality control chamber. The plasma receiving chamber, the first plasma separation chamber, and the second plasma separation chamber are connected in series. The sample detection chamber is connected in series between the first plasma separation chamber and the second plasma separation chamber. The plasma quality control chamber is connected to the first plasma separation chamber. The optical sensor assembly includes a fourth optical sensor disposed along the rotation path of the cleaning liquid quality control chamber.

10. The microfluidic luminescence detection method according to claim 1, characterized in that: The detection assembly further includes a robotic arm disposed inside the housing and a second drive component connected to the robotic arm. The second drive component is used to drive the robotic arm to move and is electrically connected to the control module. At least some of the detection elements of the detection assembly are disposed on the robotic arm.

11. The microfluidic luminescence detection method according to claim 1, characterized in that: An interactive panel is embedded in the housing. The interactive panel is electrically connected to the control module and is used to input control signals to the control module and / or to output output signals from the control module.

12. The microfluidic luminescence detection method according to claim 1, characterized in that: It also includes a printing device disposed within the housing, the printing device being electrically connected to the control module for receiving output information from the control module and printing.

13. An electronic device, characterized in that, include: One or more processors; A storage device for storing one or more programs, which, when executed by one or more processors, cause the electronic device to implement the microfluidic luminescence detection method as described in claim 1.

14. A computer-readable storage medium having a computer program stored thereon, characterized in that: When the computer program is executed by the processor, it implements the microfluidic luminescence detection method according to claim 1.

Images