A chrysosporium inermum polysaccharide degradation product and its application in protecting skin cells from thermal injury

Abstract

The application discloses a tremella aurantialba polysaccharide degradation product, which is prepared by degrading tremella aurantialba polysaccharide by a method of combining H2O2 and vitamin C and selecting degradation products with a molecular weight greater than 100 k Da and a molecular weight of 1 k Da to 100 k Da. Effective degradation of the tremella aurantialba polysaccharide can greatly improve the bioavailability of the tremella aurantialba polysaccharide, and the tremella aurantialba polysaccharide degradation product I (E-TAP I) and the tremella aurantialba polysaccharide degradation product II (E-TAP II) have good application prospects in skin thermal injury repair.

Description

TECHNICAL FIELD

[0001] The present application relates to a polysaccharide degradation product and its application, in particular to a Tremella aurantialba polysaccharide degradation product and its application in protecting skin cells from thermal injury. BACKGROUND

[0002] With the progress of technology, people have an increasing demand for effective and safe facial rejuvenation, and a variety of medical cosmetic methods have gradually been accepted and used by the public. Photothermal therapy is a widely used method in medical beauty, such as photorejuvenation, intense pulsed light, radiofrequency, laser, Thermage, etc. It mainly promotes the epidermis and dermis of our skin to change, promotes the proliferation of collagen, improves the elasticity of the skin, improves the state of the skin, reduces pores, and eliminates wrinkles and spots. However, photothermal effect can easily cause damage to the skin barrier, not only causing damage to keratinocytes, which can easily cause skin redness, dryness, and sensitivity, but also damaging dermal fibroblasts, which can reduce the ability to synthesize collagen, making the skin sagging, and in severe cases, causing skin depression. We should use the correct way to carry out medical cosmetology, and at the same time, pay attention to post-medical care, which can greatly reduce the damage to our skin.

[0003] At present, there is still a lack of research and development of postoperative soothing products related to photothermal effect-induced skin damage.

[0004] Natural skin care ingredients have always been the focus of the skin care market, and natural and green skin care ingredients are favored by consumers. In recent years, the skin care effects of edible fungi have attracted more and more attention, such as β-glucan, schizophyllan polysaccharide, lentinan, ganoderic acid, and ergothioneine from mushrooms, which have important roles in anti-inflammatory, UV protection, and antioxidant effects. Edible fungi also have good health care effects such as beauty and anti-aging. In Asia, Ganoderma, one of the edible fungi, has been used as a health care product for beauty and anti-aging for thousands of years. Edible fungi, especially mushrooms and mushroom extracts, have strong skin care effects and are increasingly being applied to the field of cosmetics.

[0005] Tremella aurantialba is a rare and precious edible fungus. According to Chinese Medicinal Fungi, Tremella aurantialba has warm and cold properties, and a sweet taste, and can reduce phlegm, stop coughing, and treat lung heat, excessive phlegm, and asthma. At the same time, due to its delicate, white, and lubricating properties, and its pleasant aroma, Tremella aurantialba has the effect of moisturizing the skin. Tremella aurantialba polysaccharide is the main component of Tremella aurantialba, and has certain anti-inflammatory, skin immune regulation, antioxidant, and moisturizing effects. SUMMARY

[0006] Invention purposes: The purpose of the present application is to provide a kind of gold ear polysaccharide degradation product, by the method of degradation of H2O2 Joint vitamin C, and by the method of ultrafiltration fractionation, gold ear polysaccharide degradation product I (E-TAP I) and gold ear polysaccharide degradation product II (E-TAP II) of two different molecular weights are obtained.The purpose of the present application is to provide the application of gold ear polysaccharide degradation product in protecting or repairing heat damaged skin cells.

[0007] Technical scheme: The gold ear polysaccharide degradation product described in the present application is degraded by the method of H2O2 Joint vitamin C, and the degradation product with molecular weight >100k Da and 1k Da < molecular weight <100k Da is selected.

[0008] The application of the gold ear polysaccharide degradation product in preparing drugs for protecting or repairing heat damaged skin.

[0009] The application, the gold ear polysaccharide degradation product includes gold ear polysaccharide degradation product I and gold ear polysaccharide degradation product II.

[0010] The application, the fractionation after the degradation of the gold ear polysaccharide is carried out by the method of ultrafiltration fractionation.

[0011] The application, the molecular weight of the gold ear polysaccharide degradation product I is >100k Da.

[0012] The application, the molecular weight of the gold ear polysaccharide degradation product II is greater than 1k Da and less than 100k Da.

[0013] The application of the gold ear polysaccharide degradation product in preparing skin care products or cosmetics for protecting or repairing heat damaged skin.

[0014] The application of gold ear polysaccharide degradation product I in preparing products for repairing heat caused keratinocyte damage, the gold ear polysaccharide degradation product I is degraded by the method of H2O2 Joint vitamin C, and the degradation product with molecular weight >100k Da is selected.

[0015] The application of gold ear polysaccharide degradation product II in preparing products for repairing heat caused fibroblast damage, the gold ear polysaccharide degradation product II is degraded by the method of H2O2 Joint vitamin C, and the degradation product with 1k Da < molecular weight <100k Da is selected.

[0016] The preparation method of gold ear polysaccharide degradation product, characterized in that, it comprises the following steps:

[0017] (1) take crude gold ear polysaccharide and dissolve in water, swell fully, add hydrogen peroxide and ascorbic acid;

[0018] (2) dialysis with a dialysis membrane with a molecular weight cut-off of 1000 Da, and the cut-off fraction is subjected to ultrafiltration fractionation with a 100 kDa ultrafiltration tube to obtain a gold ear polysaccharide degradation product I with a molecular weight greater than 100 kDa and a gold ear polysaccharide degradation product II with a molecular weight less than 100 kDa.

[0019] In a model of heat-damaged skin cells, the degradation products of gold ear polysaccharide were used as active ingredients, and the degradation products of gold ear polysaccharide with different molecular weights were applied to protect the heat damage of the stratum corneum, dermis cells and tissues of the skin. Among them, the gold ear polysaccharide with different molecular weights was degraded by the method of H2O2 combined with vitamin C, and two gold ear polysaccharide degradation products I (E-TAP I) and II (E-TAP II) with different molecular weights were prepared by ultrafiltration fractionation.

[0020] The two degradation gold ear polysaccharides and the undegraded gold ear polysaccharide were subjected to in vitro antioxidant activity analysis, and it was found that the degradation gold ear polysaccharides with different molecular weights had excellent reducing power, DPPH free radical scavenging capacity and ABTS free radical scavenging capacity. When the polysaccharide concentration was 2.0 mg / ml, the reducing power, DPPH free radical scavenging capacity and ABTS free radical scavenging capacity of E-TAP I increased by 30-35%, 45-50% and 35-40% compared with those of the undegraded polysaccharide; the reducing power, DPPH free radical scavenging capacity and ABTS free radical scavenging capacity of E-TAP II increased by 65-70%, 60-65% and 45-50% compared with those of the undegraded polysaccharide. The total antioxidant capacity of E-TAP II was greater than that of E-TAP I, and the antioxidant capacity was much greater than that of the commercially available tremella polysaccharide, indicating that the two degradation gold ear polysaccharides with different molecular weights had certain antioxidant capacity and could reduce the degree of oxidative stress caused by heat to the skin. E-TAP I could significantly enhance the proliferation and migration of human keratinocytes, and greatly increase the survival rate of keratinocytes after heat shock compared with the non-drug group. The results of this experiment suggest that E-TAP I can relieve the damage of human keratinocytes caused by heat. E-TAP II can significantly enhance the increase of fibroblasts and the survival rate of fibroblasts after heat shock. The results of this experiment suggest that E-TAP II can relieve the damage of fibroblasts caused by heat.

[0021] The existing research on gold ear polysaccharide generally focuses on the extraction and research of polysaccharide in gold ear fruiting body, but the polysaccharide extracted from the fruiting body has a large molecular weight and is difficult to pass through the stratum corneum to the dermis to exert the soothing effect. Effective degradation of gold ear polysaccharide can greatly improve the bioavailability of gold ear polysaccharide, and the degradation products of gold ear polysaccharide with different molecular weights can exert different effects on different skin layers.

[0022] The key points of the present application are:

[0023] 1. Different molecular weight polysaccharides E-TAP I and E-TAP II obtained by degrading Auricularia polyglucoside have antioxidant activity.

[0024] 2. E-TAP I can repair heat-induced damage to keratinocytes.

[0025] 3. E-TAP II can repair heat-induced damage to fibroblasts.

[0026] Advantages: Compared with the prior art, the present application has the following technical advantages: the different molecular weight Auricularia polysaccharides described in the present application can be used as active ingredients and applied in photothermal medical and beauty aftercare products, which opens up new uses of Auricularia polysaccharides and provides new options for medical and beauty aftercare products. Specifically, the application of Auricularia polysaccharides with different molecular weights in medical and beauty skin care; the application of Auricularia polysaccharides with different molecular weights in repair and soothing products; the application of Auricularia polysaccharides with different molecular weights in cosmetics. BRIEF DESCRIPTION OF DRAWINGS

[0027] Figure 1 Measurement of reducing power of different types of polysaccharides;

[0028] Figure 2 Measurement of DPPH free radical scavenging ability of different types of polysaccharides;

[0029] Figure 3 Measurement of ABTS free radical scavenging ability of different types of polysaccharides;

[0030] Figure 4 Effect of E-TAP I on the proliferation activity of keratinocytes (12h);

[0031] Figure 5 Effect of E-TAP I on the proliferation activity of keratinocytes (24h);

[0032] Figure 6 Effect of E-TAP I on the proliferation activity of keratinocytes (48h);

[0033] Figure 7 Heat damage reversal effect of E-TAP I on HaCaT;

[0034] Figure 8 Effect of E-TAP I on the migration ability of HaCaT;

[0035] Figure 9 Effect of E-TAP II on the proliferation activity of fibroblasts (12h);

[0036] Figure 10Effect of E-TAP II on the proliferation activity of fibroblasts (24h);

[0037] Figure 11 Effect of E-TAP II on the proliferation activity of fibroblasts (48h);

[0038] Figure 12 Effect of E-TAP II on the thermal damage reversal of NIH-3T3. DETAILED DESCRIPTION

[0039] In previous studies, the inventors prepared a Chrysosporium merdarium polysaccharide from Chrysosporium merdarium. The Chrysosporium merdarium polysaccharide used in the experiment was extracted from Chrysosporium merdarium and had a total sugar content of 93.66%. The Chrysosporium merdarium polysaccharide is also referred to as Chrysosporium merdarium polysaccharide in the following example section. The Chrysosporium merdarium fruiting body was dried and crushed. 75-95% ethanol was added at room temperature with stirring for 20-30 hours at a solid-liquid ratio of 1:10-1:20 g:mL or kg:L. The filter cake was dried by filtration. The defatted dry powder was obtained. The defatted dry powder was added to water and stirred until the defatted dry powder swelled. The pH was adjusted to 5.5-6.5. Cellulase and pectinase were added at a temperature of 45-55°C. The enzyme was incubated at 45-55°C for 30-45 min. Water extraction was performed at 75-80°C for 0.5-1.5 h. The supernatant was obtained by centrifugation. The supernatant was concentrated by evaporation. The concentration of anhydrous ethanol was adjusted to 75-80%. The precipitate was obtained by centrifugation after alcohol precipitation at 4-8°C for 20-30 h. The precipitate was dried. The specific extraction method is described in CN202111271004.X.

[0040] Tremella polysaccharide was provided by Shanghai Huiwen Biotechnology Co., Ltd.

[0041] Example 1 Preparation method of Chrysosporium merdarium polysaccharides with different molecular weights

[0042] Method for degrading Chrysosporium merdarium polysaccharide: 1 g of Chrysosporium merdarium polysaccharide was dissolved in 100 ml of water, swelled, and an appropriate amount of hydrogen peroxide and an equal proportion of ascorbic acid (7.5 mM) was added. The mixture was heated in a water bath at 50°C for 4 hours. After cooling, the pH was adjusted to 7.0. The mixture was dialyzed for 48 hours using a dialysis membrane with a molecular weight cutoff of 1000. The mixture was fractionated by ultrafiltration using a 100 kDa ultrafiltration tube to obtain Chrysosporium merdarium polysaccharide degradation product I (E-TAP I) with a molecular weight greater than 100 kDa and Chrysosporium merdarium polysaccharide degradation product II (E-TAP II) with a molecular weight less than 100 kDa.

[0043] Example 2 Measurement of reducing power of Chrysosporium merdarium polysaccharides with different molecular weights

[0044] Take 7.2 g of dipotassium hydrogen phosphate dodecahydrate and 3.1 g of potassium dihydrogen phosphate dihydrate, respectively dissolved in 100 mL of distilled water, mixed in a ratio of 2:3 to make a 0.2 mM PBS solution, 1 mL of 0.25, 0.5, 1.0, 1.5, 2.0 mM E-TAPI sample, E-TAP II sample, Auricularia polytricha polysaccharide (TAP) sample and Tremella polysaccharide (WSK) sample, mixed with 2.5 mL of 0.2 mM PBS solution and 2.5 mL of 1% potassium ferricyanide solution, avoid light, 50°C water bath for 20 minutes. Then add 5 mL of 10% trichloroacetic acid solution, centrifuge for 10 minutes, take 2.5 mL of supernatant, add 2.5 mL of distilled water and 0.5 mL of 0.1% ferric trichloride solution, avoid light for 10 minutes, measure the absorbance at 700 nm wavelength.

[0045] The experimental results are shown in Table 1. Figure 1

[0046] As can be seen from the figure, the reducing power of the four polysaccharides is: E-TAP II > E-TAP I > TAP > WSK. Compared with TAP, the reducing power of E-TAP I and E-TAP II is significantly improved (**p<0.01, ***p<0.001), when the polysaccharide concentration is 2 mg / ml, the reducing power of E-TAP II (average value) is 2.1 times that of E-TAP I, 3.1 times that of TAP, and 4.3 times that of Tremella polysaccharide. The results show that E-TAP II has excellent reducing power.

[0047] Example 3 Measurement of DPPH free radical scavenging capacity of Auricularia polysaccharides with different molecular weights

[0048] Take a certain amount of DPPH and dissolve it in anhydrous ethanol to make a 0.2 mM DPPH solution. Prepare 1 mL of 0.25, 0.5, 1.0, 1.5, 2.0 mM E-TAP I sample, E-TAP II sample, Auricularia polysaccharide sample and Tremella polysaccharide sample respectively, add 1 mL of DPPH solution and 1 mL of anhydrous ethanol respectively, the blank control group uses anhydrous ethanol instead of sample, i.e. 1 mL of anhydrous ethanol and 1 mL of DPPH solution, shake and mix well, and react in a 37°C water bath for 30 minutes in the dark, measure the absorbance at 517 nm wavelength. The DPPH scavenging capacity of Auricularia polysaccharide and its degradation products is calculated according to the following formula:

[0049]

[0050] In the formula, Ai is the absorbance of the experimental group

[0051] A i0 is the absorbance of the control group

[0052] A0 is the absorbance of the blank control group​

[0053] The experimental results are shown in Table 1. Figure 2

[0054] As can be seen from the figure, the DPPH free radical scavenging capacity of the four samples is: E-TAP II > E-TAP I > TAP > WSK. Compared with TAP, the DPPH free radical scavenging capacity of E-TAP I and E-TAP II is significantly increased (***p<0.001). When the concentration of polysaccharide is 2 mg / ml, the scavenging rate (average value) of E-TAP II is 1.5 times that of E-TAP I, 2.8 times that of TAP, and 5.6 times that of tremella polysaccharide. The results show that E-TAP II has excellent DPPH free radical scavenging capacity.

[0055] Example 4 Measurement of ABTS free radical scavenging capacity of different molecular weight tremella polysaccharides

[0056] ABTS solution with a concentration of 7 mmol / L and potassium persulfate solution with a concentration of 140 mmol / L were accurately prepared. The two were mixed in a certain proportion, and after being placed in the dark for 12 hours, the ABTS absorbance was adjusted to 0.7 (734 nm) with anhydrous ethanol. 1 mL of E-TAP I sample, E-TAP II sample, tremella polysaccharide sample and tremella polysaccharide sample with concentrations of 0.25, 0.5, 1.0, 1.5 and 2.0 mM respectively were taken, and each 1 mL was mixed with 1 mL of ABTS and 1 mL of distilled water. The blank control group was replaced with distilled water, and after shaking and shaking, it was placed in the dark for 1 hour, and the absorbance was measured at 734 nm. The ABTS scavenging capacity of tremella polysaccharide and its degradation products was calculated according to the following formula:

[0057]

[0058] A i —absorbance of the experimental group

[0059] A i0 —absorbance of the control group

[0060] A0—absorbance of the blank control group

[0061] The experimental results are shown in Table 1. Figure 3

[0062] ​​As can be seen from the figure, the ABTS free radical scavenging capacity of the four polysaccharides is: E-TAP II > E-TAP I > TAP > WSK. Compared with TAP, the ABTS free radical scavenging capacity of E-TAP I and E-TAP II is significantly increased (***p<0.001). When the polysaccharide concentration is 2 mg / ml, the clearance rate (average value) of E-TAP II is 1.2 times that of E-TAP I, 1.9 times that of TAP, and 3.1 times that of tremella polysaccharide. The results show that E-TAP I has excellent ABTS free radical scavenging capacity.

[0063] Example 5 Cell proliferation experiment of tremella degradation product I (E-TAP I) on keratinocytes (HaCaT)

[0064] HaCaT cells were inoculated into a 96-well plate and cultured in a 37°C 5% CO2 incubator for 24 hours. After observing the cell density and morphology under a microscope, the old culture medium was aspirated, and 25, 50, 100, 150, and 200 μg / mL of E-TAP I were added for treatment, and the cells were cultured in a 37°C 5% CO2 incubator for 12 hours. CCK-8 was used for incubation, and a blank control group was set to zero. The absorbance was measured at 450 nm using a microplate reader. The same method was used to culture and measure the cells for 24 and 48 hours.

[0065]

[0066] The cell survival rates at 12h, 24h, and 48h are shown in Tables 12, 13, and 14, respectively. Figure 4 、 5 、6.

[0067] As can be seen from the figure, at 12h, E-TAP-I at a concentration of 50 μg / mL had a very significant effect on cell proliferation (***p<0.001), and at concentrations of 100 μg / mL and 150 μg / mL, it also had a very significant effect on cell proliferation activity (**p<0.01). At 24h, a concentration of 200 μg / mL had a significant effect on cell proliferation (*p<0.05), and other concentrations had no significant effect. At 48h, E-TAP I at a concentration of 50 μg / mL had a very significant effect on cell proliferation (***p<0.01), and at a concentration of 25 μg / mL, it had a significant effect (*p<0.05). Overall, E-TAP I in the range of 25-200 μg / ml had no toxicity to keratinocytes and had a certain proliferative effect.

[0068] Example 6 Heat damage reversal effect of tremella degradation product I (E-TAP I) on HaCaT

[0069] First, the cells inoculated into the 96-well plate were grouped into control group (37°C culture group), drug administration group (E-TAPI), modeling group (48°C modeling group), modeling plus drug administration group (48°C+E-TAPI), and incubated in a 37°C, 5% CO2 constant temperature incubator for 24 hours. After 24 hours of starvation with serum-free MEM medium, the culture dishes were sealed with sealing film, and the corresponding group of culture dishes was added to the 37°C and 48°C water bath incubator for 30 minutes. The bottom of the culture dish should be completely in contact with the water surface. The corresponding concentration of serum-free MEM medium containing E-TAPI was added to the culture dish after modeling, and incubated in the incubator for 24 hours. CCK-8 was used for incubation, and a blank control group was set to zero, and the absorbance was measured at 450 nm using a microplate reader.

[0070] The experimental results are shown in Figure 7

[0071] As can be seen from the figure, 48°C can significantly reduce the survival rate of HaCaT cells (***p<0.001), and compared with the modeling group, the addition of 25, 50 μg / ml of E-TAPI can significantly improve the survival rate of HaCaT (p<0.05). The experimental results show that E-TAPI can reverse the damage to keratinocytes caused by heat.

[0072] Example 7 Cell migration experiment of Auricularia auricula degradation product I (E-TAPI) on keratinocytes (HaCaT)

[0073] HaCaT cells were inoculated in a 6-well cell culture plate at a concentration of 1.0×105 / mL, and the culture medium was complete MEM medium, 2 mL per well. Incubate in a 5% CO2, 37°C constant temperature incubator until the cell fusion rate is 100%, then use a 10 μL gun head to make a cell scratch. Add E-TAPI to the MEM basic medium without bovine serum to prepare sample solutions with concentrations of 25, 50, and 100 μg / mL (using the MEM basic medium without serum as a blank control), and incubate them with the cells for 24 hours. At 0h and 24h, observe and take photos under a microscope (0h corresponds to the original area, and 24 corresponds to the area after scratching). Calculate the migration rate (%) according to the following formula:

[0074]

[0075] The experimental results are shown in Figure 8

[0076] ​​According to the figure, compared with the Control group, E-TAP I at concentrations of 12.5, 25, 50, and 100 μg / mL had a significant promoting effect on keratinocyte migration (***p<0.001) and the promoting ability increased in a concentration-dependent manner, indicating that the degradation product of Tremella aurantialba had a promoting effect on the repair of keratinocyte damage.

[0077] Example 8 Cell proliferation experiment of fibroblasts (NIH-3T3) by degradation product II (E-TAP II) of Tremella aurantialba

[0078] NIH-3T3 cells were inoculated into a 96-well plate and cultured in a 37°C 5% CO2 incubator for 24 hours. After observing the cell density and morphology under a microscope, the old culture medium was aspirated and 25, 50, 100, 150, and 200 μg / mL of E-TAP II were added for treatment. The cells were cultured in a 37°C 5% CO2 incubator for 12 hours, incubated with CCK-8, and a blank control group was set to zero. The absorbance was measured at 450 nm using a microplate reader. The same method was used to culture the cells for 24 and 48 hours for measurement.

[0079]

[0080] The cell survival rates at 12, 24, and 48 hours are shown in Table 11. Figure 9 、 10

[0081] After 12 and 48 hours of administration, 200 μg / mL of E-TAP II had a promoting effect on the proliferation of NIH-3T3 cells, and there was a significant difference (*p<0.05). After 24 hours of administration, 50, 100, and 150 μg / mL of E-TAP II had a promoting effect on the proliferation of NIH-3T3 cells, and there was a significant difference (*p<0.05, ***p<0.001). Overall, E-TAP II had no toxicity to keratinocytes and had a certain promoting effect on the proliferation of keratinocytes within the range of 25-200 μg / mL.

[0082] Example 9 Heat damage reversal effect of degradation product II (E-TAP II) of Tremella aurantialba on fibroblasts (NIH-3T3)

[0083] ​First, the cells inoculated into the 96-well plate were grouped into control group (37℃), drug administration group (E-TAP II), modeling group (48℃), modeling plus drug administration group (48℃+E-TAP II), and cultured in a 37℃ 5% CO2 constant temperature incubator for 24 hours. Then, serum-free DMEM medium was added for starvation for 24 hours. The culture dishes were sealed with sealing film, and the corresponding group of culture dishes was added to a 37℃, 48℃ water bath incubator for 10 minutes, and the bottom of the culture dish should be completely in contact with the water surface. The corresponding concentration of serum-free DMEM medium containing E-TAP II was added to the culture dish after modeling, and incubated in an incubator for 24 hours. CCK-8 was used for incubation, and a blank control group was set to zero. The absorbance was measured at 450nm using a microplate reader.

[0084] The experimental results are shown in Figure 12

[0085] As can be seen from the figure, 48℃ can significantly reduce the survival rate of NIH-3T3 cells (***p<0.001), and compared with the 48℃ group without E-TAP II, the addition of 50, 100μg / ml E-TAP I can significantly improve the survival rate of NIH-3T3 cells (***p<0.001). The experimental results show that E-TAP II can reverse the damage to fibroblasts caused by heat.​

Claims

1. Use of the degradation product Ⅱ of Tremella aurantialba polysaccharide in the preparation of a product for repairing thermal-induced fibroblast damage, wherein the degradation product Ⅱ of Tremella aurantialba polysaccharide is degraded from Tremella aurantialba polysaccharide by a method of H2O2 combined with vitamin C, and the degradation product with a molecular weight of 1 k Da < molecular weight < 100 k Da is selected.

2. Use according to claim 1, characterized in that, The preparation method of the degradation product Ⅱ of Tremella aurantialba polysaccharide comprises the following steps: (1) Dissolve the crude Tremella aurantialba polysaccharide in water, swell thoroughly, and add hydrogen peroxide and ascorbic acid; (2) Perform dialysis with a dialysis membrane with a molecular weight cut-off of 1000 Da, and perform ultrafiltration fractionation on the cut-off part with a 100 kDa ultrafiltration tube to obtain the degradation product Ⅰ of Tremella aurantialba polysaccharide with a molecular weight greater than 100 k Da and the degradation product Ⅱ of Tremella aurantialba polysaccharide with a molecular weight less than 100 k Da.

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