Method and system for constructing an animal experimental model of ophthalmic diseases

Abstract

The present invention proposes a method and system for constructing an animal experimental model for ophthalmic diseases. The construction method includes: determining experimental animals based on experimental requirements and dividing the experimental animals into multiple experimental groups and a control group; wherein the experimental groups are used for ophthalmic disease experiments, and the control group is used to maintain a normal state for comparison; setting laser irradiation information and inducing glaucoma in the small animals in the experimental group using laser induction; determining the animal experimental model based on ocular data obtained from regular eye examinations of the experimental group, and analyzing the eyeballs of the animal experimental model to obtain experimental analysis results. The system includes modules corresponding to the steps of the method.

Description

Technical Field

[0001] The present invention provides a method and system for constructing an animal experimental model of ophthalmological diseases, belonging to the technical field of animal experimental modeling. Background Art

[0002] In-depth research and understanding of ophthalmic diseases are the basis for building animal experimental models. Research results on the characteristics, pathological mechanisms, and development processes of ophthalmic diseases provide important background knowledge for building models. For specific ophthalmic diseases, suitable experimental animals are selected as models for research. Commonly used ophthalmic disease model animals include mice, rats, rabbits, etc., and appropriate animal species are selected according to the characteristics and pathological mechanisms of the disease. According to the characteristics of the target ophthalmic disease, appropriate induction methods are developed to simulate and replicate the disease process. For example, laser induction, drug injection, genetic alteration, etc. can be used to induce the simulation of ophthalmic diseases. However, the animal experimental models in the existing technology have the problems of long determination cycle and low efficiency. Summary of the Invention

[0003] The present invention provides a method and system for constructing an animal experimental model for ophthalmic diseases, which is used to solve the problems of long determination period and low efficiency of animal experimental models in the prior art.

[0004] A method for constructing an animal experimental model of an ophthalmic disease, the method comprising:

[0005] Determine experimental animals according to the experimental requirements, and divide the experimental animals into multiple experimental groups and a control group; wherein the experimental groups are used for conducting ophthalmic disease experiments, and the control group is used to maintain a normal state for comparison;

[0006] Setting laser irradiation information and inducing glaucoma in the animals in the experimental group using laser induction;

[0007] According to the eye data obtained by regularly performing eye examinations on the experimental group, an animal experimental model is determined, and the eyeballs of the animal experimental model are analyzed to obtain experimental analysis results.

[0008] Furthermore, the experimental animals are determined according to the experimental requirements, and the experimental animals are divided into multiple experimental groups and a control group, including:

[0009] Extracting the experimental requirements input by the experimental user terminal and determining the experimental animals according to the experimental requirements;

[0010] Determine the number of experimental groups and the number of animals in each experimental group based on the experimental animals; wherein the number of experimental groups is an even number greater than or equal to 6 and a multiple of 3;

[0011] A control group is set up according to the experimental animals, and the number of animals in the control group is set.

[0012] Furthermore, setting the laser irradiation information includes:

[0013] Setting the laser irradiation area;

[0014] Selecting a pair of experimental groups as a first experimental group pair, and setting laser parameters for the first experimental group pair to obtain first laser parameters; wherein the laser parameter setting includes laser irradiation power setting and laser irradiation time setting;

[0015] selecting a pair of experimental groups different from the first experimental group pair as a second experimental group pair, and setting second laser parameters according to the first laser parameters;

[0016] selecting a pair of experimental groups different from the first experimental group pair and the second experimental group pair as a third experimental group pair, and setting third laser parameters according to the first laser parameters and the third laser parameters;

[0017] Traversing all unpaired experimental groups to perform pairing processing to form multiple experimental group pairs except the first experimental group pair, the second experimental group pair, and the third experimental group pair;

[0018] Parameters are set in sequence for multiple experimental group pairs except the first experimental group pair, the second experimental group pair, and the third experimental group pair according to the first laser parameters, the second laser parameters, and the third laser parameters.

[0019] Furthermore, the first laser parameter, the second laser parameter and the third laser parameter are obtained by the following formula:

[0020]

[0021]

[0022] Wherein, P1 represents the laser irradiation power in the first laser parameter; P0 is the preset initial reference laser power; n represents the number of cumulative unit times experienced in the historical use record of the laser induction device, and the value of the unit time is 1s; S0 represents the irradiable area of ​​the animal eye; S represents the set laser irradiation area of ​​the current experimental group, and, when S=S0, let S-S0=1; ΔS represents the maximum allowable area difference between the preset irradiable area of ​​the animal eye and the set laser irradiation area of ​​the current experimental group; S d represents the minimum area value of the laser irradiation area of ​​the current experimental group that meets the experimental requirements; λ1 and λ2 represent the first adjustment coefficient and the second adjustment coefficient, respectively. At the same time, the value ranges of λ1 and λ2 are 0.92-0.97 and 1.08-1.13, respectively; P zirepresents the total output power of the laser induction device per unit time; ΔP zi Indicates the maximum power fluctuation amplitude allowed in the experiment; T1 represents the laser irradiation time in the first laser parameter; T0 represents the initial reference irradiation time corresponding to the preset initial reference laser power;

[0023]

[0024]

[0025] Wherein, P2 represents the laser irradiation power in the second laser parameter; T2 represents the laser irradiation time in the second laser parameter;

[0026]

[0027]

[0028] Wherein, P3 represents the laser irradiation power in the third laser parameter; T3 represents the laser irradiation time in the third laser parameter.

[0029] Furthermore, based on the eye data obtained from regular eye examinations of the experimental group, an animal experimental model is determined, and the eyeballs of the animal experimental model are analyzed to obtain experimental analysis results, including:

[0030] Based on the eye data obtained by regularly performing eye examinations on the experimental groups, a pair of experimental groups with the same disease trend are selected as animal experimental models, wherein the eye examinations include intraocular pressure measurement, corneal thickness measurement and retinal imaging;

[0031] At a pre-set specified time, molecular analysis, mathematical analysis and interpretation are performed on the corresponding information of the tissue slices of the eyeballs removed from the mice in the animal experimental model to obtain the experimental analysis results.

[0032] A system for constructing an animal experimental model of an ophthalmic disease, the system comprising:

[0033] An experimental information preparation module is used to determine experimental animals according to the experimental requirements and divide the experimental animals into multiple experimental groups and a control group; wherein the experimental groups are used to conduct ophthalmic disease experiments, and the control group is used to maintain a normal state for comparison;

[0034] The equipment parameter setting module is used to set the laser irradiation information and use the laser induction method to induce glaucoma in the small animals in the experimental group;

[0035] The animal experiment model determination module is used to determine the animal experiment model based on the eye data obtained by regular eye examinations of the experimental group, and to analyze the eyeballs of the animal experiment model to obtain experimental analysis results.

[0036] Furthermore, the experimental information preparation module includes:

[0037] An information extraction module is used to extract the experimental requirements input by the experimental user terminal and determine the experimental animals according to the experimental requirements;

[0038] A first information determination module is configured to determine the number of experimental groups and the number of animals in each experimental group based on the experimental animals; wherein the number of experimental groups is an even number greater than or equal to 6 and a multiple of 3;

[0039] The second information determination module is used to set up a control group according to the experimental animals and set the number of animals in the control group.

[0040] Furthermore, the device parameter setting module includes:

[0041] An area setting module is used to set the laser irradiation area;

[0042] A first parameter acquisition module is used to select a pair of experimental groups as a first experimental group pair, and set laser parameters for the first experimental group pair to obtain first laser parameters; wherein the laser parameter setting includes laser irradiation power setting and laser irradiation time setting;

[0043] a second parameter acquisition module, configured to select a pair of experimental groups different from the first experimental group pair as a second experimental group pair, and set second laser parameters according to the first laser parameters;

[0044] a third parameter acquisition module, configured to select a pair of experimental groups different from the first experimental group pair and the second experimental group pair as a third experimental group pair, and set third laser parameters according to the first laser parameters and the third laser parameters;

[0045] a teaming module, configured to traverse all unpaired experimental groups and perform pairing processing to form a plurality of experimental group pairs other than the first experimental group pair, the second experimental group pair, and the third experimental group pair;

[0046] The parameter assigning module is used to sequentially set parameters for multiple experimental groups other than the first experimental group, the second experimental group and the third experimental group according to the first laser parameter, the second laser parameter and the third laser parameter.

[0047] Furthermore, the first laser parameter, the second laser parameter and the third laser parameter are obtained by the following formula:

[0048]

[0049]

[0050] Wherein, P1 represents the laser irradiation power in the first laser parameter; P0 is the preset initial reference laser power; n represents the number of cumulative unit times experienced in the historical use record of the laser induction device, and the value of the unit time is 1s; S0 represents the irradiable area of ​​the animal eye; S represents the set laser irradiation area of ​​the current experimental group, and, when S=S0, let S-S0=1; ΔS represents the maximum allowable area difference between the preset irradiable area of ​​the animal eye and the set laser irradiation area of ​​the current experimental group; S d represents the minimum area value of the laser irradiation area of ​​the current experimental group that meets the experimental requirements; λ1 and λ2 represent the first adjustment coefficient and the second adjustment coefficient, respectively. At the same time, the value ranges of λ1 and λ2 are 0.92-0.97 and 1.08-1.13, respectively; P zi represents the total output power of the laser induction device per unit time; ΔP zi Indicates the maximum power fluctuation amplitude allowed in the experiment; T1 represents the laser irradiation time in the first laser parameter; T0 represents the initial reference irradiation time corresponding to the preset initial reference laser power;

[0051]

[0052]

[0053] Wherein, P2 represents the laser irradiation power in the second laser parameter; T2 represents the laser irradiation time in the second laser parameter;

[0054]

[0055]

[0056] Wherein, P3 represents the laser irradiation power in the third laser parameter; T3 represents the laser irradiation time in the third laser parameter.

[0057] Furthermore, the animal experiment model determination module includes:

[0058] a model determination module, configured to select a pair of experimental groups with the same disease trend as animal experimental models based on eye data obtained by regularly performing eye examinations on the experimental groups, wherein the eye examinations include intraocular pressure measurement, corneal thickness measurement, and retinal imaging;

[0059] The experimental data analysis module is used to perform molecular analysis, mathematical analysis and interpretation on the corresponding information of the tissue slices of the eyeballs removed from the mice in the animal experimental model at a pre-set specified time to obtain the experimental analysis results.

[0060] Beneficial effects of the present invention:

[0061] The method and system for constructing an animal experimental model of an ophthalmic disease proposed in the present invention successfully induce a glaucoma disease model in experimental animals through laser induction, simulating the pathological characteristics and pathological process of human glaucoma. Through regular eye examinations and data acquisition, the progression of glaucoma in experimental animals can be evaluated, including increased intraocular pressure, changes in eyeball morphology, etc., to understand the impact of the disease. By analyzing the eyeballs of the animal experimental model, experimental analysis results on the pathological changes of glaucoma can be obtained, such as changes in the tissue structure of the eyeball, changes at the molecular level, etc., providing an experimental basis for studying the pathogenesis and treatment methods of glaucoma. The above-mentioned technical scheme proposed in the present invention can successfully construct an animal experimental model of glaucoma disease and provide experimental analysis results related to the disease, providing important experimental basis and reference for the research and treatment of glaucoma. BRIEF DESCRIPTION OF THE DRAWINGS

[0062] Figure 1 A flow chart of the method of the present invention;

[0063] Figure 2 This is a system block diagram of the system of the present invention. DETAILED DESCRIPTION

[0064] The preferred embodiments of the present invention are described below with reference to the accompanying drawings. It should be understood that the preferred embodiments described herein are only used to illustrate and explain the present invention, and are not used to limit the present invention.

[0065] The present invention provides a method for constructing an animal experimental model of ophthalmological diseases, such as Figure 1 As shown, the construction method includes:

[0066] S1. Determine experimental animals according to the experimental requirements, and divide the experimental animals into multiple experimental groups and a control group; wherein the experimental groups are used for ophthalmic disease experiments, and the control group is used to maintain a normal state for comparison;

[0067] S2. setting laser irradiation information and inducing glaucoma in the animals in the experimental group using laser induction;

[0068] S3. Determine an animal experimental model based on the eye data obtained from regular eye examinations of the experimental group, and analyze the eyeballs of the animal experimental model to obtain experimental analysis results.

[0069] The working principle of the above technical solution is as follows: according to the experimental needs, suitable experimental animals are selected as research subjects and divided into experimental and control groups. Laser irradiation technology is used to induce glaucoma in the eyes of the small animals in the experimental group. Laser induction causes damage to the optical fibers on the cornea, thereby obstructing the drainage of aqueous humor and increasing intraocular pressure. Regular eye examinations are performed on the experimental group to collect eye data, such as intraocular pressure and corneal morphology, to monitor and evaluate whether the animals in the experimental group develop symptoms of glaucoma. Based on the eye data obtained from the eye examination, the animal experimental model is determined, that is, whether glaucoma is successfully induced in the animals in the experimental group. Various methods and technical means can be used to analyze the eyeballs of the animal experimental model, such as tissue sections, biochemical analysis, etc., to obtain experimental analysis results on the pathological changes of glaucoma.

[0070] The technical effect of the above technical solution is as follows: The above technical solution of this embodiment successfully induces a glaucoma model in experimental animals through laser induction, simulating the pathological characteristics and pathological process of human glaucoma. Through regular eye examinations and data acquisition, the progression of glaucoma in experimental animals can be assessed, including elevated intraocular pressure and changes in eye morphology, to understand the impact of the disease. By analyzing the eyes of the animal experimental models, experimental analysis results on the pathological changes of glaucoma, such as changes in ocular tissue structure and molecular changes, can be obtained, providing experimental basis for studying the pathogenesis and treatment of glaucoma.

[0071] The above technical solution proposed in this embodiment can successfully construct an animal experimental model of glaucoma disease and provide experimental analysis results related to the disease, providing important experimental basis and reference for the research and treatment of glaucoma.

[0072] In one embodiment of the present invention, experimental animals are determined according to the experimental requirements, and the experimental animals are divided into multiple experimental groups and a control group, including:

[0073] S101, extracting experimental requirements input by an experimental user terminal, and determining experimental animals according to the experimental requirements;

[0074] S102. Determine the number of experimental groups and the number of animals in each experimental group based on the experimental animals; wherein the number of experimental groups is an even number greater than or equal to 6 and a multiple of 3;

[0075] S103. Setting up a control group according to the experimental animals and setting the number of animals in the control group.

[0076] The working principle of the above technical solution is as follows: key information, such as the desired ophthalmic disease and the experimental purpose, is extracted from the experimental requirements input by the user terminal. Based on the experimental requirements, suitable experimental animals are determined as research subjects, taking into account the needs of the experimental groups. The number of experimental groups and the number of animals in each experimental group are determined based on the characteristics of the experimental animals and the experimental requirements. At the same time, a control group is set up to compare and contrast the results of the experimental groups.

[0077] The technical effects of the above technical solution are: by extracting experimental requirements and determining experimental animals, the experimental plan is ensured to meet the user's experimental needs and research objectives. The number of subjects and animals in the experimental and control groups is determined according to the specified conditions, ensuring the rationality and reliability of the experimental design. By stipulating that the number of experimental groups is an even number that is greater than or equal to 6 and a multiple of 3, and by setting up a control group, a balance between the experimental and control groups is achieved, making the experimental results more comparable and interpretable.

[0078] In summary, the technical solution of this embodiment can meet the experimental needs, design reasonable experimental and control groups, and achieve balance between the experimental and control groups.

[0079] In one embodiment of the present invention, setting the laser irradiation information includes:

[0080] S201, setting a laser irradiation area;

[0081] S202, selecting a pair of experimental groups as a first experimental group pair, and setting laser parameters for the first experimental group pair to obtain first laser parameters; wherein the laser parameter setting includes laser irradiation power setting and laser irradiation time setting;

[0082] S203, selecting a pair of experimental groups different from the first experimental group pair as a second experimental group pair, and setting second laser parameters according to the first laser parameters;

[0083] S204, selecting a pair of experimental groups different from the first experimental group pair and the second experimental group pair as a third experimental group pair, and setting third laser parameters according to the first laser parameters and the third laser parameters;

[0084] S205, traversing all unpaired experimental groups to perform pairing processing to form multiple experimental group pairs except the first experimental group pair, the second experimental group pair, and the third experimental group pair;

[0085] S206 , setting parameters for multiple experimental pairs except the first experimental pair, the second experimental pair, and the third experimental pair in sequence according to the first laser parameter, the second laser parameter, and the third laser parameter.

[0086] The first laser parameter, the second laser parameter and the third laser parameter are obtained by the following formula:

[0087]

[0088]

[0089] Wherein, P1 represents the laser irradiation power in the first laser parameter; P0 is the preset initial reference laser power; n represents the number of cumulative unit times experienced in the historical use record of the laser induction device, and the value of the unit time is 1s; S0 represents the irradiable area of ​​the animal eye; S represents the set laser irradiation area of ​​the current experimental group, and, when S=S0, let S-S0=1; ΔS represents the maximum allowable area difference between the preset irradiable area of ​​the animal eye and the set laser irradiation area of ​​the current experimental group; S d represents the minimum area value of the laser irradiation area of ​​the current experimental group that meets the experimental requirements; λ1 and λ2 represent the first adjustment coefficient and the second adjustment coefficient, respectively. At the same time, the value ranges of λ1 and λ2 are 0.92-0.97 and 1.08-1.13, respectively; P zi represents the total output power of the laser induction device per unit time; ΔP zi Indicates the maximum power fluctuation amplitude allowed in the experiment; T1 represents the laser irradiation time in the first laser parameter; T0 represents the initial reference irradiation time corresponding to the preset initial reference laser power;

[0090]

[0091]

[0092] Wherein, P2 represents the laser irradiation power in the second laser parameter; T2 represents the laser irradiation time in the second laser parameter;

[0093]

[0094]

[0095] Wherein, P3 represents the laser irradiation power in the third laser parameter; T3 represents the laser irradiation time in the third laser parameter.

[0096] The working principle of this technical solution is to determine the specific area to be irradiated by the laser during the experiment to ensure accurate and consistent irradiation. Laser parameters, including irradiation power and duration, are then gradually set based on the selected experimental pairs to obtain specific laser parameters for each experimental pair. By iterating through the unpaired experimental pairs, the previously set laser parameters are then applied to each of these experimental pairs.

[0097] The technical effect of the above technical solution is: by clearly defining the specific area of ​​laser irradiation, the accuracy and consistency of irradiation in the experiment are ensured. By gradually setting the laser parameters of different experimental groups, personalized laser irradiation is achieved for each experimental group. By selecting different experimental groups and targeted laser parameter settings, multiple experimental groups are formed to investigate the effects of different laser parameters on ophthalmic disease experiments. At the same time, the first laser parameters, the second laser parameters, and the third laser parameters set in the above manner can effectively reduce the length of the laser parameter debugging cycle, thereby improving the parameter debugging efficiency and acquisition efficiency of the first laser parameters, the second laser parameters, and the third laser parameters. At the same time, the first laser parameters, the second laser parameters, and the third laser parameters obtained in the above manner can maximize the matching between the parameter settings and the actual experiment, further improving the accuracy and efficiency of the animal experimental model.

[0098] In summary, the technical solution of this embodiment provides a more accurate and targeted research plan for ophthalmic disease experiments through precise laser irradiation area setting, laser parameter setting for multiple experimental groups, and differential treatment of different experimental groups.

[0099] In one embodiment of the present invention, an animal experimental model is determined based on eye data obtained by regularly performing eye examinations on the experimental group, and the eyeballs of the animal experimental model are analyzed to obtain experimental analysis results, including:

[0100] S301. Based on the ocular data obtained by regularly performing ocular examinations on the experimental groups, a pair of experimental groups with the same disease trend are selected as animal experimental models, wherein the ocular examinations include intraocular pressure measurement, corneal thickness measurement, and retinal imaging;

[0101] S302: Perform molecular analysis, mathematical analysis, and interpretation on the tissue slices of the eyeballs removed from the mice in the animal experimental model at a predetermined time to obtain experimental analysis results.

[0102] The working principle of this technical solution is as follows: Regular eye examinations are performed on the experimental group, including intraocular pressure measurement, corneal thickness measurement, and retinal imaging, to obtain ocular data to monitor disease progression and trends. Based on the ocular data obtained from these examinations, pairs of experimental groups with similar disease trends are selected as animal experimental models. The ocular data of these experimental groups reflect similar disease characteristics and progression. At predetermined time points, tissue sections are taken from the mouse eyes removed from the animal experimental models, and molecular and mathematical analyses are performed to obtain the experimental analysis results.

[0103] The technical effect of the above technical solution is that, through ocular examination and data analysis, experimental groups with similar disease trends are selected as animal experimental models, making the research more comparable and interpretable. By performing tissue sectioning and molecular analysis on the removed mouse eyeballs, combined with mathematical analysis and interpretation, experimental analysis results about the disease, such as pathological changes and changes at the molecular level, are obtained.

[0104] In summary, the above technical solution of this embodiment provides an experimental basis and reference for the study of ophthalmic diseases by establishing an animal experimental model with similar disease trends and obtaining experimental analysis results.

[0105] The embodiment of the present invention provides a system for constructing an animal experimental model of ophthalmological diseases, such as Figure 2 As shown, the construction system includes:

[0106] An experimental information preparation module is used to determine experimental animals according to the experimental requirements and divide the experimental animals into multiple experimental groups and a control group; wherein the experimental groups are used to conduct ophthalmic disease experiments, and the control group is used to maintain a normal state for comparison;

[0107] The equipment parameter setting module is used to set the laser irradiation information and use the laser induction method to induce glaucoma in the small animals in the experimental group;

[0108] The animal experiment model determination module is used to determine the animal experiment model based on the eye data obtained by regular eye examinations of the experimental group, and to analyze the eyeballs of the animal experiment model to obtain experimental analysis results.

[0109] The working principle of the above technical solution is as follows: according to the experimental needs, suitable experimental animals are selected as research subjects and divided into experimental and control groups. Laser irradiation technology is used to induce glaucoma in the eyes of the small animals in the experimental group. Laser induction causes damage to the optical fibers on the cornea, thereby obstructing the drainage of aqueous humor and increasing intraocular pressure. Regular eye examinations are performed on the experimental group to collect eye data, such as intraocular pressure and corneal morphology, to monitor and evaluate whether the animals in the experimental group develop symptoms of glaucoma. Based on the eye data obtained from the eye examination, the animal experimental model is determined, that is, whether glaucoma is successfully induced in the animals in the experimental group. Various methods and technical means can be used to analyze the eyeballs of the animal experimental model, such as tissue sections, biochemical analysis, etc., to obtain experimental analysis results on the pathological changes of glaucoma.

[0110] The technical effect of the above technical solution is as follows: The above technical solution of this embodiment successfully induces a glaucoma model in experimental animals through laser induction, simulating the pathological characteristics and pathological process of human glaucoma. Through regular eye examinations and data acquisition, the progression of glaucoma in experimental animals can be assessed, including elevated intraocular pressure and changes in eye morphology, to understand the impact of the disease. By analyzing the eyes of the animal experimental models, experimental analysis results on the pathological changes of glaucoma, such as changes in ocular tissue structure and molecular changes, can be obtained, providing experimental basis for studying the pathogenesis and treatment of glaucoma.

[0111] The above technical solution proposed in this embodiment can successfully construct an animal experimental model of glaucoma disease and provide experimental analysis results related to the disease, providing important experimental basis and reference for the research and treatment of glaucoma.

[0112] In one embodiment of the present invention, the experimental information preparation module includes:

[0113] An information extraction module is used to extract the experimental requirements input by the experimental user terminal and determine the experimental animals according to the experimental requirements;

[0114] A first information determination module is configured to determine the number of experimental groups and the number of animals in each experimental group based on the experimental animals; wherein the number of experimental groups is an even number greater than or equal to 6 and a multiple of 3;

[0115] The second information determination module is used to set up a control group according to the experimental animals and set the number of animals in the control group.

[0116] The working principle of the above technical solution is as follows: key information, such as the desired ophthalmic disease and the experimental purpose, is extracted from the experimental requirements input by the user terminal. Based on the experimental requirements, suitable experimental animals are determined as research subjects, taking into account the needs of the experimental groups. The number of experimental groups and the number of animals in each experimental group are determined based on the characteristics of the experimental animals and the experimental requirements. At the same time, a control group is set up to compare and contrast the results of the experimental groups.

[0117] The technical effects of the above technical solution are: by extracting experimental requirements and determining experimental animals, the experimental plan is ensured to meet the user's experimental needs and research objectives. The number of subjects and animals in the experimental and control groups is determined according to the specified conditions, ensuring the rationality and reliability of the experimental design. By stipulating that the number of experimental groups is an even number that is greater than or equal to 6 and a multiple of 3, and by setting up a control group, a balance between the experimental and control groups is achieved, making the experimental results more comparable and interpretable.

[0118] In summary, the technical solution of this embodiment can meet the experimental needs, design reasonable experimental and control groups, and achieve balance between the experimental and control groups.

[0119] In one embodiment of the present invention, the device parameter setting module includes:

[0120] An area setting module is used to set the laser irradiation area;

[0121] A first parameter acquisition module is used to select a pair of experimental groups as a first experimental group pair, and set laser parameters for the first experimental group pair to obtain first laser parameters; wherein the laser parameter setting includes laser irradiation power setting and laser irradiation time setting;

[0122] a second parameter acquisition module, configured to select a pair of experimental groups different from the first experimental group pair as a second experimental group pair, and set second laser parameters according to the first laser parameters;

[0123] a third parameter acquisition module, configured to select a pair of experimental groups different from the first experimental group pair and the second experimental group pair as a third experimental group pair, and set third laser parameters according to the first laser parameters and the third laser parameters;

[0124] a teaming module, configured to traverse all unpaired experimental groups and perform pairing processing to form a plurality of experimental group pairs other than the first experimental group pair, the second experimental group pair, and the third experimental group pair;

[0125] The parameter assigning module is used to sequentially set parameters for multiple experimental groups other than the first experimental group, the second experimental group and the third experimental group according to the first laser parameter, the second laser parameter and the third laser parameter.

[0126] The first laser parameter, the second laser parameter and the third laser parameter are obtained by the following formula:

[0127]

[0128]

[0129] Wherein, P1 represents the laser irradiation power in the first laser parameter; P0 is the preset initial reference laser power; n represents the number of cumulative unit times experienced in the historical use record of the laser induction device, and the value of the unit time is 1s; S0 represents the irradiable area of ​​the animal eye; S represents the set laser irradiation area of ​​the current experimental group, and, when S=S0, let S-S0=1; ΔS represents the maximum allowable area difference between the preset irradiable area of ​​the animal eye and the set laser irradiation area of ​​the current experimental group; S drepresents the minimum area value of the laser irradiation area of ​​the current experimental group that meets the experimental requirements; λ1 and λ2 represent the first adjustment coefficient and the second adjustment coefficient, respectively. At the same time, the value ranges of λ1 and λ2 are 0.92-0.97 and 1.08-1.13, respectively; P zi represents the total output power of the laser induction device per unit time; ΔP zi Indicates the maximum power fluctuation amplitude allowed in the experiment; T1 represents the laser irradiation time in the first laser parameter; T0 represents the initial reference irradiation time corresponding to the preset initial reference laser power;

[0130]

[0131]

[0132] Wherein, P2 represents the laser irradiation power in the second laser parameter; T2 represents the laser irradiation time in the second laser parameter;

[0133]

[0134]

[0135] Wherein, P3 represents the laser irradiation power in the third laser parameter; T3 represents the laser irradiation time in the third laser parameter.

[0136] The working principle of this technical solution is to determine the specific area to be irradiated by the laser during the experiment to ensure accurate and consistent irradiation. Laser parameters, including irradiation power and duration, are then gradually set based on the selected experimental pairs to obtain specific laser parameters for each experimental pair. By iterating through the unpaired experimental pairs, the previously set laser parameters are then applied to each of these experimental pairs.

[0137] The technical effect of the above technical solution is to ensure the accuracy and consistency of irradiation during experiments by clearly defining the specific areas for laser irradiation. By gradually setting laser parameters for different experimental pairs, personalized laser irradiation can be achieved for each experimental pair. By selecting different experimental pairs and setting targeted laser parameters, multiple experimental pairs can be formed to examine the effects of different laser parameters on ophthalmic disease experiments.

[0138] In summary, the technical solution of this embodiment provides a more accurate and targeted research plan for ophthalmic disease experiments through precise laser irradiation area setting, laser parameter setting for multiple experimental groups, and differential treatment of different experimental groups.

[0139] In one embodiment of the present invention, the animal experiment model determination module includes:

[0140] a model determination module, configured to select a pair of experimental groups with the same disease trend as animal experimental models based on eye data obtained by regularly performing eye examinations on the experimental groups, wherein the eye examinations include intraocular pressure measurement, corneal thickness measurement, and retinal imaging;

[0141] The experimental data analysis module is used to perform molecular analysis, mathematical analysis and interpretation on the corresponding information of the tissue slices of the eyeballs removed from the mice in the animal experimental model at a pre-set specified time to obtain the experimental analysis results.

[0142] The working principle of this technical solution is as follows: Regular eye examinations are performed on the experimental group, including intraocular pressure measurement, corneal thickness measurement, and retinal imaging, to obtain ocular data to monitor disease progression and trends. Based on the ocular data obtained from these examinations, pairs of experimental groups with similar disease trends are selected as animal experimental models. The ocular data of these experimental groups reflect similar disease characteristics and progression. At predetermined time points, tissue sections are taken from the mouse eyes removed from the animal experimental models, and molecular and mathematical analyses are performed to obtain the experimental analysis results.

[0143] The technical effect of the above technical solution is that, through ocular examination and data analysis, experimental groups with similar disease trends are selected as animal experimental models, making the research more comparable and interpretable. By performing tissue sectioning and molecular analysis on the removed mouse eyeballs, combined with mathematical analysis and interpretation, experimental analysis results about the disease, such as pathological changes and changes at the molecular level, are obtained.

[0144] In summary, the above technical solution of this embodiment provides an experimental basis and reference for the study of ophthalmic diseases by establishing an animal experimental model with similar disease trends and obtaining experimental analysis results.

[0145] Obviously, those skilled in the art may make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if such changes and modifications fall within the scope of the claims and their equivalents, the present invention is intended to include such changes and modifications.

Claims

1. A method for constructing an animal experimental model of an ophthalmic disease, characterized in that: The construction method comprises: Determine experimental animals according to experimental requirements, and divide the experimental animals into multiple experimental groups and a control group; wherein the experimental groups are used for ophthalmic disease experiments, and the control group is used to maintain a normal state for comparison; Setting laser irradiation information and inducing glaucoma in the animals in the experimental group using laser induction; Determining an animal experimental model based on the eye data obtained by regularly performing eye examinations on the experimental group, and analyzing the eyeballs of the animal experimental model to obtain experimental analysis results; Setting the laser irradiation information includes: Setting the laser irradiation area; Selecting a pair of experimental groups as a first experimental group pair, and setting laser parameters for the first experimental group pair to obtain first laser parameters; wherein the laser parameter setting includes laser irradiation power setting and laser irradiation time setting; selecting a pair of experimental groups different from the first experimental group pair as a second experimental group pair, and setting second laser parameters according to the first laser parameters; selecting a pair of experimental groups different from the first experimental group pair and the second experimental group pair as a third experimental group pair, and setting third laser parameters according to the first laser parameters and the third laser parameters; Traversing all unpaired experimental groups to perform pairing processing to form multiple experimental group pairs except the first experimental group pair, the second experimental group pair, and the third experimental group pair; Setting parameters for multiple experimental groups other than the first experimental group, the second experimental group, and the third experimental group in sequence according to the first laser parameter, the second laser parameter, and the third laser parameter; The first laser parameter, the second laser parameter and the third laser parameter are obtained by the following formula: in, P 1 represents the laser irradiation power in the first laser parameter; P 0 is the preset initial reference laser power; n Indicates the number of accumulated unit times in the historical usage record of the laser induction device, and the value of the unit time is 1s; S 0 means the area of ​​the animal's eyes that can be irradiated; S Indicates the laser irradiation area of ​​the current experimental group, and when S = S 0 o'clock, order S - S 0=1;Δ S Indicates the maximum allowable area difference between the preset animal eye irradiation area and the set laser irradiation area of ​​the current experimental group; S d Indicates the minimum area value of the laser irradiation area of ​​the current experimental group that meets the experimental requirements; λ 1 and λ 2 represents the first adjustment coefficient and the second adjustment coefficient respectively. At the same time, λ 1 and λ The values ​​of 2 range from 0.92 to 0.97 and 1.08 to 1.13 respectively; P zi Indicates the i The total output power of the laser induction device per unit time; Δ P zi Indicates the maximum power fluctuation allowed in the experiment; T 1 represents the laser irradiation time in the first laser parameter; T 0 represents the initial reference irradiation time corresponding to the preset initial reference laser power; in, P 2 represents the laser irradiation power in the second laser parameter; T 2 represents the laser irradiation time in the second laser parameter; in, P 3 represents the laser irradiation power in the third laser parameter; T 3 represents the laser irradiation time in the third laser parameter.

2. The construction method according to claim 1, characterized in that: The experimental animals are determined according to the experimental requirements and divided into multiple experimental groups and a control group, including: Extracting the experimental requirements input by the experimental user terminal and determining the experimental animals according to the experimental requirements; Determine the number of experimental groups and the number of animals in each experimental group based on the experimental animals; wherein the number of experimental groups is an even number greater than or equal to 6 and a multiple of 3; A control group is set up according to the experimental animals, and the number of animals in the control group is set.

3. The construction method according to claim 1, characterized in that: Based on the eye data obtained from regular eye examinations of the experimental group, an animal experimental model is determined, and the eyeballs of the animal experimental model are analyzed to obtain experimental analysis results, including: Based on the eye data obtained by regularly performing eye examinations on the experimental groups, a pair of experimental groups with the same disease trend are selected as animal experimental models, wherein the eye examinations include intraocular pressure measurement, corneal thickness measurement and retinal imaging; At a pre-set specified time, molecular analysis, mathematical analysis and interpretation are performed on the corresponding information of the tissue slices of the eyeballs removed from the mice in the animal experimental model to obtain the experimental analysis results.

4. A system for constructing an animal experimental model of an ophthalmic disease, characterized in that: The build system includes: An experimental information preparation module is used to determine experimental animals according to experimental requirements and divide the experimental animals into multiple experimental groups and a control group; wherein the experimental groups are used for ophthalmic disease experiments, and the control group is used to maintain a normal state for comparison; The equipment parameter setting module is used to set the laser irradiation information and use the laser induction method to induce glaucoma in the small animals in the experimental group; An animal experimental model determination module is used to determine the animal experimental model based on the eye data obtained by regular eye examinations of the experimental group, and to analyze the eyeballs of the animal experimental model to obtain experimental analysis results; The device parameter setting module includes: An area setting module is used to set the laser irradiation area; A first parameter acquisition module is used to select a pair of experimental groups as a first experimental group pair, and set laser parameters for the first experimental group pair to obtain first laser parameters; wherein the laser parameter setting includes laser irradiation power setting and laser irradiation time setting; a second parameter acquisition module, configured to select a pair of experimental groups different from the first experimental group pair as a second experimental group pair, and set second laser parameters according to the first laser parameters; a third parameter acquisition module, configured to select a pair of experimental groups different from the first experimental group pair and the second experimental group pair as a third experimental group pair, and set third laser parameters according to the first laser parameters and the third laser parameters; a teaming module, configured to traverse all unpaired experimental groups and perform pairing processing to form a plurality of experimental group pairs other than the first experimental group pair, the second experimental group pair, and the third experimental group pair; a parameter assigning module, configured to sequentially set parameters for a plurality of experimental pairs other than the first experimental pair, the second experimental pair, and the third experimental pair according to the first laser parameter, the second laser parameter, and the third laser parameter; The first laser parameter, the second laser parameter and the third laser parameter are obtained by the following formula: in, P 1 represents the laser irradiation power in the first laser parameter; P 0 is the preset initial reference laser power; n Indicates the number of accumulated unit times in the historical usage record of the laser induction device, and the value of the unit time is 1s; S 0 means the area of ​​the animal's eyes that can be irradiated; S Indicates the laser irradiation area of ​​the current experimental group, and when S = S 0 o'clock, order S - S 0=1;Δ S Indicates the maximum allowable area difference between the preset animal eye irradiation area and the set laser irradiation area of ​​the current experimental group; S d Indicates the minimum area value of the laser irradiation area of ​​the current experimental group that meets the experimental requirements; λ 1 and λ 2 represents the first adjustment coefficient and the second adjustment coefficient respectively. At the same time, λ 1 and λ The values ​​of 2 range from 0.92 to 0.97 and 1.08 to 1.13 respectively; P zi Indicates the i The total output power of the laser induction device per unit time; Δ P zi Indicates the maximum power fluctuation allowed in the experiment; T 1 represents the laser irradiation time in the first laser parameter; T 0 represents the initial reference irradiation time corresponding to the preset initial reference laser power; in, P 2 represents the laser irradiation power in the second laser parameter; T 2 represents the laser irradiation time in the second laser parameter; in, P 3 represents the laser irradiation power in the third laser parameter; T 3 represents the laser irradiation time in the third laser parameter.

5. The construction system according to claim 4, characterized in that: The experimental information preparation module includes: An information extraction module is used to extract the experimental requirements input by the experimental user terminal and determine the experimental animals according to the experimental requirements; A first information determination module is configured to determine the number of experimental groups and the number of animals in each experimental group based on the experimental animals; wherein the number of experimental groups is an even number greater than or equal to 6 and a multiple of 3; The second information determination module is used to set up a control group according to the experimental animals and set the number of animals in the control group.

6. The construction system according to claim 4, characterized in that: The animal experiment model determination module includes: a model determination module, configured to select a pair of experimental groups with the same disease trend as animal experimental models based on eye data obtained by regularly performing eye examinations on the experimental groups, wherein the eye examinations include intraocular pressure measurement, corneal thickness measurement, and retinal imaging; The experimental data analysis module is used to perform molecular analysis, mathematical analysis and interpretation on the corresponding information of the tissue slices of the eyeballs removed from the mice in the animal experimental model at a pre-set specified time to obtain the experimental analysis results.

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