A kind of pink spiral polysporus water dispersible granules and preparation method thereof

Abstract

This invention provides a water-dispersible granule of *Polyporus pulveratus* and its preparation method. The water-dispersible granule comprises *Polyporus pulveratus* spores, active metabolites, fillers, and adjuvants. The filler is one or more of diatomaceous earth, bentonite, light calcium carbonate, kaolin, and talc. The adjuvants include wetting agents, dispersants, disintegrants, and binders. The *Polyporus pulveratus* water-dispersible granule proposed in this invention contains *Polyporus pulveratus* spores and active metabolites, and can control plant diseases through multiple mechanisms such as parasitism, competition, antagonism, and production of cell wall hydrolytic enzymes. It also exhibits high safety and stable performance.

Description

Technical Field

[0001] This invention belongs to the field of microbial technology, specifically relating to a pink spiral polyspora preparation, a preparation method for the preparation, and its application. Background Technology

[0002] Plant diseases seriously affect the healthy development of agriculture in my country. Currently, chemical control is still the main method in production, but the long-term excessive use of chemical pesticides has led to a series of problems such as environmental pollution, pesticide residues, and increased pesticide resistance. Biological control has the advantages of being non-toxic, residue-free, environmentally friendly, and having a long-lasting effect, and is playing an increasingly important role in disease control.

[0003] *Clonostachys rosea*, also known as pink broom fungus, is a highly effective biocontrol fungus. Widely distributed in soil, this fungus can parasitize various plant pathogenic fungi and nematodes. It is characterized by rapid reproduction, strong adaptability, and a broad antibacterial spectrum. It controls crop diseases through multiple mechanisms, including parasitism, competition, antibiotic and toxin production, secretion of degrading enzymes, and induction of plant resistance. It can also promote plant growth, demonstrating significant potential in disease control. Currently, the research and development of *Clonostachys rosea* is receiving increasing attention, with two biopesticide products already registered abroad. *Clonostachys rosea* inoculants are mostly in powder and wettable powder form, but issues such as poor formulation and efficacy stability still exist, limiting their large-scale production and application.

[0004] Water-dispersible granules, also known as dry suspensions, are mainly composed of active ingredients, wetting agents, dispersants, binders, disintegrants, and fillers processed and granulated. These formulations have a high content of active ingredients and rapidly disintegrate into a uniform suspension upon addition of water. They exhibit good dispersibility, strong flowability, and stable disease prevention effects. Furthermore, they effectively solve the dust pollution problems associated with powders and wettable powders, and because they do not use organic solvents, they are safer and are currently recognized internationally as a safe and environmentally friendly dosage form. Research and development of water-dispersible granules of *Polyspora pinkis* will promote its industrialization and large-scale application. Summary of the Invention

[0005] The first objective of this invention is to provide a water-dispersible granule of *Polyspora pinkis* to improve the stability and application efficacy of *Polyspora pinkis* agents.

[0006] The second objective of this invention is to provide a method for preparing the aforementioned pink spiral polyspora water-dispersible granules.

[0007] A third objective of this invention is to propose the application of the aforementioned pink spiral polyspora water-dispersible granules.

[0008] The technical solution to achieve the above-mentioned objective of the present invention is as follows: a water-dispersible granule of *Polyspora pinkis*, composed of *Polyspora pinkis* spores, active metabolites, fillers and additives; the filler is one or more of diatomaceous earth, bentonite, light calcium carbonate, kaolin, and talc, and the additives include wetting agents, dispersants, disintegrants and binders.

[0009] During cultivation, *Alternaria pinki* produces various antibiotic and toxic metabolites, such as mycotoxins, mycotoxins, polypeptides, and piperpoxytoxins, exhibiting broad-spectrum antibacterial activity. These metabolites inhibit pathogen growth, spore and sclerotium germination, and also suppress plant nematodes. Simultaneously, *Alternaria pinki* secretes cell wall hydrolases such as chitinase, glucanase, and cellulase, degrading pathogen cell walls, inhibiting pathogen growth and spread, and controlling plant diseases. Furthermore, *Alternaria pinki* produces auxin-like substances that significantly promote plant growth, increase fruit set, and improve yield.

[0010] The bacterial content in the water-dispersible granules is (1-6)×10⁻⁶. 8 CFU / g.

[0011] The water-dispersible granules contain 1-5% of the spores and active metabolites of *Polyspora pinkis* and 65-85% of the filler.

[0012] Further, the wetting agent is one or more of sodium dodecyl sulfate, sodium dodecylbenzene sulfonate, sucrose fatty acid ester, and stretching powder, and the amount added is 1-6% of the mass of the water-dispersible granules; the dispersant is one or more of sodium carboxymethyl cellulose, sodium lignosulfonate, sodium tripolyphosphate, Tween 80, and calcium lignosulfonate, and the amount added is 3-10% of the mass of the water-dispersible granules; the disintegrant is one or more of urea, sodium carboxymethyl starch, ammonium sulfate, sodium sulfate, and calcium chloride, and the amount added is 3-10% of the mass of the water-dispersible granules; the binder is one or more of polyvinyl alcohol, polyethylene glycol 4000, polyethylene glycol 6000, and soluble starch, and the amount added is 1-6% of the mass of the water-dispersible granules.

[0013] Preferably, the filler accounts for 72-80% of the mass of the water-dispersible granules.

[0014] More preferably, the wetting agent is sucrose fatty acid ester, accounting for 2-4% of the total mass of the water-dispersible granules; the dispersant is sodium carboxymethyl cellulose, accounting for 5-7% of the mass; the disintegrant is sodium sulfate, accounting for 6-8% of the mass; and the binder is soluble starch, accounting for 3-5% of the mass.

[0015] The present invention also proposes a method for preparing the aforementioned *Polyspora pinkis* water-dispersible granules, comprising the following steps:

[0016] 1) Preparation of spore suspension: Inoculate *Polyspora pinkis* onto PDA medium and culture at 25–28°C for 6–8 days. Add sterile water to wash away the spores and prepare a spore suspension.

[0017] 2) Seed culture: Add seed culture medium to the Erlenmeyer flask, sterilize by high temperature and moist heat, and when it cools to room temperature, inoculate the spore suspension into the Erlenmeyer flask and culture at 25-28℃ with shaking for 48-72h to obtain the seed culture.

[0018] 3) Liquid fermentation culture: Prepare fermentation culture medium, sterilize and cool it, then inoculate with seed liquid and culture at 25-28℃ for 48-96 hours to obtain fermentation broth.

[0019] 4) Thoroughly mix the filler with wetting agent, dispersant, disintegrant and binder, add fermentation broth, stir, granulate and dry to obtain water-dispersible granules.

[0020] The liquid fermentation culture medium formula in step 3) is as follows: 15-40g glucose, 5-10g yeast extract, 5-15g soybean meal powder, 0.2-1g MgSO4·7H2O, 0.5-2g KH2PO4, add water to 1000mL, adjust the pH to 5.5-6.5 with hydrochloric acid, and sterilize to obtain the fermentation culture medium.

[0021] The liquid fermentation culture conditions in step 3) are as follows: inoculum size 0.5-5% (V / V), rotation speed 150-250 r / min, temperature 25-28℃, and culture time 48-96 h.

[0022] A preferred embodiment of the present invention is as follows: In step 4), the filler is thoroughly mixed with wetting agent, dispersant, disintegrant and binder to form a solid material, and then added to the fermentation broth. The mass ratio of the fermentation broth to the solid material is 1:1 to 2.3.

[0023] Generally, the solid content of the dried fungal fermentation broth is 3-5% of the broth's mass.

[0024] Application of the pink spiral polyspora water-dispersible granules of this invention in the control of gray mold, sclerotinia rot, wilt, root rot and blight in crops.

[0025] The mass parts described in this invention can be grams, kilograms, jin (a Chinese unit of weight), tons, or other mass units defined for ease of operation. Within the same formulation, "parts" represent the same unit mass. The formulations described in this invention can be scaled up or down proportionally, or prepared into liquid or solid culture media using methods known in the art.

[0026] The beneficial effects of this invention are as follows:

[0027] The *Polyporus pulveratum* water-dispersible granules proposed in this invention contain *Polyporus pulveratum* spores and active metabolites, and can control plant diseases through multiple mechanisms of action, including parasitism, competition, antagonism, and production of cell wall hydrolytic enzymes. Furthermore, compared with conventional powders and granules, *Polyporus pulveratum* water-dispersible granules have a higher bacterial content, higher safety, and more stable performance.

[0028] The water-dispersible granules prepared using this method have good biocompatibility with *Polyspora pinkis*, and the additives used improve the cell suspension rate, facilitating the release of active ingredients. Furthermore, the granules have strong hardness, which helps *Polyspora pinkis* to better exert its disease prevention effect.

[0029] The control effect of pink spiral polyspora water-dispersible granules on gray mold of cucumber was determined in a greenhouse. The results showed that after spraying with a 500-fold dilution, the number of lesions on cucumber leaves was significantly reduced, and the control efficacy against gray mold reached 44.4%; after spraying with a 200-fold dilution, almost no lesions were observed on cucumber leaves, and the control efficacy reached 81.1%. Attached Figure Description

[0030] Figure 1 Granulation equipment used in the research and development of pink spiral polyspora water-dispersible granules.

[0031] Figure 2 The finished product of pink spiral polyspora water-dispersible granules. Detailed Implementation

[0032] The following examples are used to illustrate the present invention, but are not intended to limit the scope of the invention.

[0033] Unless otherwise specified, all test materials and instruments used in this instruction manual are commercially available.

[0034] Unless otherwise specified, all parts in the examples are by weight, and all percentages are by weight percentages.

[0035] Example 1: Liquid fermentation of *Polyspora pinkis*

[0036] This embodiment uses the *Clonostachys rosea* GW3-1 strain (CGMCC No. 18152, deposited on August 9, 2019, at the China General Microbiological Culture Collection Center, No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing). The strain was inoculated into PDA medium and cultured at 26°C for 7 days. 5 mL of sterile water was added to wash away the spores. The spore suspension was then inoculated into PD medium and cultured at 26°C and 180 rpm for 48 hours to obtain the seed culture.

[0037] Fermentation medium was prepared with the following formula: 35g glucose, 5g yeast extract, 10g soybean meal, 1g K₂HPO₄, 0.5g MgSO₄·7H₂O, and water was added to 1000mL. The pH was adjusted to 6.0 with 1M hydrochloric acid. After sterilization and cooling, the culture was inoculated into the seed culture at a rate of 2%, and cultured in a shaker at 28℃ for 76h to obtain the fermentation broth.

[0038] The spore count in the fermentation broth using a hemocytometer was 3.36 × 10⁻⁶. 8 / mL.

[0039] Example 2: Screening of fillers for pink spiral polyspora water-dispersible granules

[0040] The screening of fillers and additives was conducted using single-factor experiments. In this example:

[0041] Diatomaceous earth, bentonite, calcium carbonate, kaolin, and talc were selected as fillers for water-dispersible granules, respectively, and mixed with the fermentation broth of *Polyporus pulveratus* prepared in Example 1. The effects of different fillers on the survival of *Polyporus pulveratus* spores and their adsorption capacity were determined, and the best carrier was screened (the compatibility and adsorption capacity with the bacteria are characteristics of the filler (carrier) itself and are not related to other adjuvants).

[0042] The results showed that the type of packing material had a significant impact on the spore activity of *Alternaria pinki* fermentation broth (P<0.05). Among them, the addition of diatomaceous earth resulted in the highest number of viable bacteria, which was 3.35 × 10⁻⁶. 8 The highest concentration of CFU / mL was found in kaolin, followed by kaolin clay with a colony count of 3.05 × 10⁻⁶. 8 With a CFU / mL concentration, the survival rate of other fillers added was less than 60%, which had a significant impact and poor compatibility. Furthermore, diatomaceous earth had the highest adsorption capacity at 4.5 mL / g, while kaolin had an adsorption capacity of only 1.57 mL / g. Therefore, diatomaceous earth was chosen as the filler for the pink spiral polyspora water-dispersible granules.

[0043] Example 3: Screening of wetting agents in *Polyspora pinkis* water-dispersible granules

[0044] Four wetting agents (sodium dodecyl sulfate, sodium dodecylbenzene sulfonate, sucrose fatty acid ester, and puller) were added to the pink spiral polyspora fermentation broth prepared in Example 1 at 2% and 5% of the mass of the water-dispersible granules, respectively. Diatomaceous earth was added and mixed well to prepare water-dispersible granule samples containing different types of wetting agents. Suitable wetting agents were screened based on wetting time.

[0045] The results showed that different wetting agents had significant effects on the activity and mycelial growth of *Polyspora pulmonata* (P<0.05). Among them, the addition of sucrose fatty acid ester resulted in the highest bacterial activity, with an inhibition rate of 4.3%–7.2% on mycelial growth. Sodium dodecyl sulfate, sodium dodecylbenzene sulfonate, and thiamethoxam at a concentration of 5% all had an effect on bacterial activity exceeding 80%, with inhibition rates of 30%–60% on mycelial growth. Wetting time measurements showed that when the concentration of sucrose fatty acid ester was 5%, the wetting time of the prepared water-dispersible granules was 58.6 s, and when the concentration was 2%, the wetting time was 59.3 s, significantly lower than the control without wetting agent (wetting time 99.0 s), meeting the quality standards for water-dispersible granules. Therefore, sucrose fatty acid ester was selected as the wetting agent for *Polyspora pulmonata* water-dispersible granules, with an addition amount of 2%. Other wetting agents had poor compatibility with the experimental strain and were not suitable for use in *Polyspora pulmonata* water-dispersible granules.

[0046] Example 4: Screening of dispersants in *Polyspora pinkis* water-dispersible granules

[0047] Five dispersants (sodium carboxymethyl cellulose, sodium lignosulfonate, sodium tripolyphosphate, Tween 80, and calcium lignosulfonate) were added to the *Polysporium rosenbergii* fermentation broth prepared in Example 1 at amounts of 5% and 10% of the water-dispersible granules, respectively. Diatomaceous earth was added and the mixture was stirred to prepare water-dispersible granule samples containing different types of dispersants. Suitable dispersants were screened based on suspension rate and wetting time.

[0048] The results showed that different types of dispersants had different effects on the activity and mycelial growth of *Alternaria rosenbergii* (P<0.05). Sodium carboxymethyl cellulose and sodium lignosulfonate had almost no effect on cell activity, followed by calcium lignosulfonate. The activity of the fungus treated with sodium tripolyphosphate and Tween 80 was less than 50%. Mycelial growth rate measurements showed that at concentrations of 5% and 10%, sodium carboxymethyl cellulose had no inhibitory effect on *Alternaria rosenbergii* mycelia, while sodium lignosulfonate showed inhibition rates of 2.4% and 5.5%, respectively, and calcium lignosulfonate showed inhibition rates of 4.8% and 11.9%, respectively. Furthermore, the suspension rate and wetting time of the fungal agents with 10% sodium carboxymethyl cellulose and sodium lignosulfonate were measured. The results showed that the suspension rate of the fungal agent with sodium carboxymethyl cellulose was not different from that with sodium lignosulfonate, but the wetting times were 60.7 s and 70.4 s, respectively, showing a significant difference (P<0.05). In summary, sodium carboxymethyl cellulose was selected as the dispersant for *Polyspora pinkis* water-dispersible granules. To further determine the optimal concentration of sodium carboxymethyl cellulose, concentrations of 5%, 6%, 7%, 8%, 9%, and 10% were tested. The results showed that the suspension rate of the bacterial agent was 66%–67% and the wetting time was 53.0–60.7 s, with little difference between the different concentrations. Therefore, concentrations of 5–7% were selected for subsequent orthogonal experiments.

[0049] Example 5

[0050] Screening of disintegrants from *Polyspora pinkis*

[0051] Five disintegrants (urea, sodium carboxymethyl starch, ammonium sulfate, sodium sulfate, and calcium chloride) were added to the *Polyspora pinkis* fermentation broth prepared in Example 1 at amounts of 5% and 10% of the water-dispersible granule mass, respectively. Diatomaceous earth was added and the mixture was stirred to prepare water-dispersible granule samples containing different types of disintegrants. Suitable disintegrants were screened based on disintegration time.

[0052] The results showed that the addition of sodium carboxymethyl starch and sodium sulfate had no significant effect on the activity and mycelial growth of *Polyspora pulvinata*, and exhibited good compatibility with biocontrol bacteria. The other four disintegrants, however, showed varying degrees of inhibition (7.2%–100%). When the sodium sulfate concentration was 10%, the disintegration time was 64.7 s, significantly lower than that of sodium carboxymethyl starch (78.4 s). Therefore, sodium sulfate was selected as the disintegrant for *Polyspora pulvinata* water-dispersible granules.

[0053] Example 6

[0054] Screening of pink spiral polyspora adhesives

[0055] Five binders (polyvinyl alcohol, polyethylene glycol 4000, polyethylene glycol 6000, polyethylene glycol 20000, and soluble starch) were added to the pink spiral polyspora fermentation broth prepared in Example 1 at an addition amount of 5% of the water-dispersible granules mass. Diatomaceous earth was added and mixed well to prepare water-dispersible granule samples containing different types of binders. Suitable binders were screened based on disintegration time and sample particle hardness.

[0056] The results showed that polyethylene glycol 6000 and soluble starch had no effect on the spore activity and mycelial growth of *Polyspora pinkis*, indicating good compatibility with the strain. However, the disintegration time was the shortest with the addition of soluble starch (72.34 s), significantly lower than the control (105.32 s) and polyethylene glycol 6000 (82.56 s). Furthermore, the addition of soluble starch significantly increased the particle hardness of the formulation. Further analysis of the soluble starch concentration revealed that while low concentrations (1–2%) shortened the disintegration time, they resulted in lower particle hardness, making them unsuitable for granulation.

[0057] Example 7: Screening of adjuvants for *Polyspora pinkis* water-dispersible granules (orthogonal experiment)

[0058] Steps 1) to 3) are the same as in Example 1.

[0059] 4) Thoroughly mix the filler with wetting agent, dispersant, disintegrant and binder, add fermentation broth, stir, granulate and dry to obtain water-dispersible granules.

[0060] The types and concentrations of wetting agents, disintegrants, dispersants, and adhesives screened out through single-factor experiments were then classified according to L. 16 (4 4 An orthogonal design was used to add adjuvants and diatomaceous earth to the fermentation broth of *Polyspora pinkis* prepared in Example 1 to prepare water-dispersible granules with different ratios. The suspension rate, wetting time, disintegration time and effects on bacterial activity of the formulations were determined.

[0061] The results showed significant differences in wetting time, suspension rate, disintegration time, and viable cell count among different combinations of formulations (P<0.05). The wetting time ranged from 42.3 to 53.3 s, the disintegration time from 66.3 to 80.3 s, and the suspension rate from 67.3 to 87.3%. When using 2% sucrose fatty acid ester as a wetting agent, 7% sodium carboxymethyl cellulose as a dispersant, 8% sodium sulfate as a disintegrant, and 5% soluble starch as a binder, the prepared *Polyspora pinkis* water-dispersible granules had a wetting time of 42.3 s, a disintegration time of 66.3 s, and a suspension rate of 87.7%, meeting the quality standards for water-dispersible granules. The bacterial content of the prepared formulation was 1.8 × 10⁻⁶. 8 CFU / g (Table 1).

[0062] Table 1 Orthogonal Experiment L 16 (4 4 Design and Results

[0063]

[0064] Example 8

[0065] A test of the control effect of pink spiral polyspora water-dispersible granules on cucumber gray mold.

[0066] Water-dispersible granules were prepared using the *Polyspora pinkis* fermentation broth prepared in Example 1 and the filler and adjuvant formulations obtained from Examples 2-7. The operating steps were the same as in Example 7. Specifically, in this example, solid materials were added to the fermentation broth at a mass ratio of 1:2. The filler was diatomaceous earth, accounting for 77% of the total mass of the water-dispersible granules; the wetting agent was sucrose fatty acid ester, accounting for 2% of the total mass of the water-dispersible granules; the dispersant was sodium carboxymethyl cellulose, accounting for 7% of the mass; the disintegrant was sodium sulfate, accounting for 7% of the mass; and the binder was soluble starch, accounting for 5% of the mass.

[0067] First, the filler and various additives are premixed. Then, the fermentation broth is added at a solid / liquid mass ratio of 2:1, and the mixture is thoroughly stirred and granulated using a granulator. The granulation process is then completed at 35°C to obtain the finished product. See [link to granulation equipment and finished product description] for details. Figure 1 , Figure 2 .

[0068] Soak Zhongnong No. 6 cucumber seeds in a 2% NaClO solution for 3 minutes, rinse repeatedly with sterile water, and then place them in a petri dish lined with filter paper. Add a small amount of sterile water and germinate at 28℃. After germination, sow 4 seeds per pot in sterile soil. After emergence, reduce the number of seedlings to 3 per pot. When the cucumbers have 4-5 true leaves, spray the leaves with 200-fold and 500-fold diluted pink spiral polyspora water-dispersible granules, respectively. Two hours later, spray with cucumber gray mold inoculum. Use only the pathogen fermentation broth and water as controls. Maintain humidity and culture. After 7 days, investigate the disease incidence in cucumbers, and statistically analyze the disease index and prevention effect. Based on the disease incidence, classify the severity of the disease into 4 levels (Level 0: normal leaves, no lesions; Level 1: lesion area less than 10%; Level 2: lesion area 10%-30%; Level 3: lesion area 30%-50%; Level 4: lesion area greater than 50%). Each treatment had 36 seedlings.

[0069] The results showed that the pink spiral polyspora water-dispersible granules had a good control effect on gray mold in cucumber. Seven days after inoculation, obvious lesions appeared on the cucumber leaves of the water control group. After spraying with a 500-fold dilution of the water-dispersible granules prepared in this example, the lesions on the cucumber leaves were significantly reduced, and the control efficacy against gray mold reached 44.4%. After spraying with a 200-fold dilution, there were basically no lesions on the cucumber leaves, and the control efficacy reached 81.1% (Table 2).

[0070] Table 2. Control efficacy of pink spiral polyspora water-dispersible granules against cucumber gray mold.

[0071]

[0072] Although the present invention has been described above through embodiments, those skilled in the art should understand that any improvements and modifications made to the present invention without departing from its spirit and essence should fall within the protection scope of the present invention.

Claims

1. A pink spiral polyspora water-dispersible granule, characterized in that, The product is composed of *Cyclospora pinkis* spores, active metabolites, fillers, and additives. The filler is diatomaceous earth, comprising 65-85% of the product. The additives include wetting agents, dispersants, disintegrants, and binders. The wetting agent is sucrose fatty acid ester, comprising 2-4% of the water-dispersible granules by mass. The dispersant is sodium carboxymethyl cellulose, comprising 5-7% by mass. The disintegrant is sodium sulfate, comprising 6-8% by mass. The binder is soluble starch, comprising 3-5% by mass. The bacterial content in the water-dispersible granules is (1-6) × 10⁻⁶. 8 CFU / g, with *Polyspora pinkis* spores and active metabolites comprising 1-5% of the mass of the water-dispersible granules; the *Polyspora pinkis* water-dispersible granules are prepared using the following steps: 1) Preparation of spore suspension: Inoculate *Polyspora pinkis* onto PDA medium and culture at 25–28°C for 6–8 days. Add sterile water to wash away the spores and prepare a spore suspension. 2) Seed culture: Add seed culture medium to the Erlenmeyer flask, sterilize by high temperature and moist heat, and when it cools to room temperature, inoculate the spore suspension into the Erlenmeyer flask and culture at 25-28℃ with shaking for 48-72h to obtain the seed culture. 3) Liquid fermentation culture: Prepare fermentation culture medium, sterilize and cool it, then inoculate it with seed liquid and culture at 25-28℃ for 48-96 hours to obtain fermentation broth; 4) Thoroughly mix the filler with wetting agent, dispersant, disintegrant and binder, add fermentation broth, stir, granulate and dry to obtain water-dispersible granules.

2. The pink spiral polyspora water-dispersible granules according to claim 1, characterized in that, The filler accounts for 72-80% of the mass of the water-dispersible granules.

3. The pink spiral polyspora water-dispersible granules according to claim 1, characterized in that, The culture medium formula for liquid fermentation in step 3) is as follows: 15-40g glucose, 5-10g yeast extract, 5-15g soybean meal powder, 0.2-1g MgSO4·7H2O, 0.5-2g KH2PO4, add water to 1000mL, adjust the pH to 5.5-6.5 with hydrochloric acid, and sterilize to obtain the fermentation culture medium.

4. The pink spiral polyspora water-dispersible granules according to claim 1, characterized in that, The liquid fermentation culture conditions in step 3) are as follows: inoculum size 0.5-5% (V / V), rotation speed 150-250 r / min, temperature 25-28℃, and culture time 48-96 h.

5. The pink spiral polyspora water-dispersible granules according to claim 1, characterized in that, In step 4), the filler is thoroughly mixed with wetting agent, dispersant, disintegrant and binder to form a solid material, and then added to the fermentation broth. The mass ratio of the fermentation broth to the solid material is 1:1 to 2.

3.

6. The application of the pink spiral polyspora water-dispersible granules according to any one of claims 1 to 5 in the prevention and control of gray mold, sclerotinia rot, wilt, root rot and blight in crops.

Images