Method for preparing additive of hydrothermal biomass graphene and fungus and testing method thereof

Abstract

The present application relates to provide a kind of hydrothermal biomass graphene synergistic additive preparation method and test method of fungus, it is related to soil treatment technical field.The preparation method includes obtaining straw and excrement, and preparing biochar mixture;Hydrothermal biomass graphene is generated by hydrothermal reaction to biochar mixture and graphene particles;Moss ball sac fungus is used to prepare plant cluster root fungus liquid;Hydrothermal biomass graphene is mixed with plant cluster root fungus liquid, and additive is formed.It is mixed to form additive by hydrothermal biochar synergistic graphene and plant root fungus, both realize the treatment of soil pollutants, and reach the efficient resource utilization of biochar and graphene.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of soil remediation, and particularly relates to a preparation method and a testing method of an additive of hydrothermal biomass graphene and fungi. BACKGROUND

[0002] Plant remediation of soil contaminated by polycyclic emerging pollutants has limitations: the toxicity of polycyclic emerging pollutants and the lack of water and nutrients in contaminated soil often limit the germination, growth and activity of plants; the complexity of pollutant components in the active soil and the coexistence of multiple pollutants may inhibit the growth of plants and the biomass of polycyclic emerging pollutant degrading bacteria, ultimately affecting the efficiency of the plant remediation system; the long growth cycle of plants slows down the plant remediation speed; and the plant remediation effect is easily affected by natural factors such as pests, floods, etc. This not only limits the application range of plant remediation technology, but also affects the effect of plant remediation. These problems have become the bottleneck of popularizing plant remediation technology of contaminated soil. Arbuscular mycorrhizal fungi can form a mutualistic symbiotic body with most terrestrial higher plants, and is one of the most common and oldest plant growth-promoting fungi. Since the 1980s and 1990s of last century, arbuscular mycorrhizal fungi have been introduced into plant remediation of inorganic matter represented by heavy metals and persistent organic pollutant contaminated soil, and have played a positive role in the planting and remediation of plants in contaminated soil.

[0003] The influence of biomass charcoal formed by carbonization of agricultural waste on plant growth and soil properties has been studied to some extent, and the results show that biomass charcoal has a certain promoting effect on plant growth. However, these studies are based on the direct addition of biochar to soil, and there is no relevant research report on the influence of biomass material as a compost additive on soil properties, pollutant control and plant growth together with plant arbuscular mycorrhizal fungi. Studies have shown that graphene, known as black gold, is a soil conditioner, and its effect may be doubled when it is used in combination with biomass and plant arbuscular mycorrhizal fungi. Emerging pollutants (PPCPs) are organic pollutants with high content and great harm in sewage sludge from industries such as health products, fragrances, cosmetics, sunscreens and disinfectants, and their migration characteristics in the process of sludge resource utilization are worth attention. However, current research on PPCPs in soil-plant systems is mostly blank soil spiking experiments, and the environmental behavior of PPCPs introduced by hydrothermal biomass graphene in soil-plant systems has been studied less, and mainly focuses on the influence of sludge as an organic fertilizer on vegetables, rice and other crops. There is no report on the treatment of emerging pollutants by hydrothermal biomass graphene and plant arbuscular mycorrhizal fungi in combination.

[0004] Therefore, designing a hydrothermal biomass graphene synergistic fungus additive preparation method and test method, mixing hydrothermal biochar synergistic graphene and plant root fungus to form an additive, which not only realizes the treatment of soil pollutants, but also achieves efficient resource utilization of biochar and graphene, is a problem to be solved at present. SUMMARY

[0005] Therefore, the present application aims to overcome the defects of the prior art, and provides a hydrothermal biomass graphene synergistic fungus additive preparation method, which combines biochar and graphene through hydrothermal treatment, and combines with plant arbuscular mycorrhizal fungi to form an additive with excellent effect of treating new soil pollutants, thereby achieving full resource utilization of biochar and graphene at low cost and improving the treatment effect of new soil pollutants.

[0006] The present application also provides a test method, which compares the degradation of soil emerging pollutants by the hydrothermal biomass graphene synergistic plant arbuscular mycorrhizal fungus additive, hydrothermal biomass graphene and plant arbuscular mycorrhizal fungus, to fully demonstrate the degradation effect of the hydrothermal biomass graphene synergistic plant arbuscular mycorrhizal fungus additive on soil new pollutants.

[0007] The present application provides a first technical solution:

[0008] A hydrothermal biomass graphene synergistic fungus additive preparation method, comprising obtaining straw and manure to prepare a biochar mixture; obtaining the biochar mixture and graphene particles to generate hydrothermal biomass graphene through a hydrothermal reaction; using M. mosei to prepare a plant arbuscular mycorrhizal fungus liquid; and mixing the hydrothermal biomass graphene and the plant arbuscular mycorrhizal fungus liquid to form an additive.

[0009] Further, in the preparation of the biochar mixture, the mass ratio of straw to manure is 50% to 60% for straw and 40% to 50% for manure.

[0010] Further, in the preparation of the hydrothermal biomass graphene, the mass fraction of biochar in the biochar mixture is 5% to 8%, the mass fraction of graphene in the graphene particles is 5% to 10%, and the temperature of the hydrothermal reaction is 500°C to 600°C.

[0011] Further, using M. mosei to prepare a plant arbuscular mycorrhizal fungus liquid comprises: preparing a glucose solution and cooling it to room temperature; mixing soil cultured with M. mosei and leek root hairs in the glucose solution and performing oscillation centrifugation, and screening and rinsing the oscillated solution to obtain plant arbuscular mycorrhizal fungi; counting and picking out the plant arbuscular mycorrhizal fungi, and mixing and configuring a plant arbuscular mycorrhizal fungus liquid using a low-phosphorus nutrient solution.

[0012] Further, in the process of preparing the plant arbuscular mycorrhizal fungus liquid, the concentration of the glucose solution is 450 g / L; the weight of the soil for culturing the M. neovorica is in the range of 8 g to 12 g; the volume of the glucose solution used for oscillation and centrifugation is in the range of 45 ml to 60 ml; the oscillation and centrifugation time is in the range of 4 min to 5 min; and the mass percentage of the plant arbuscular mycorrhizal fungus in the plant arbuscular mycorrhizal fungus liquid is in the range of 2% to 3%.

[0013] Further, the mixing method of the hydrothermal biomass graphene and the plant arbuscular mycorrhizal fungus liquid is as follows: the hydrothermal biomass graphene is mixed with the soil in a mass ratio in the range of 1:4 to 1:6 to form a mixture; and the plant arbuscular mycorrhizal fungus liquid is added to the mixture periodically.

[0014] The second technical solution provided by the application is as follows:

[0015] A test method, which uses the additive prepared by the first technical solution, comprises the following steps: obtaining a first comparison object, i.e., the hydrothermal biomass graphene; obtaining a second comparison object, i.e., the plant arbuscular mycorrhizal fungus liquid; mixing the additive with test soil, and planting three pots of alfalfa and three pots of kaya; mixing the first comparison object with the test soil, and planting three pots of alfalfa and three pots of kaya; mixing the second comparison object with the test soil, and planting three pots of alfalfa and three pots of kaya; determining the biomass of the alfalfa and the kaya during the planting period, and comparing the content of PPCPs and the transfer amount of PPCPs; and determining the composition of the test soil during the planting period, and comparing the content of PPCPs.

[0016] The application has the following beneficial effects:

[0017] The additive preparation method of the hydrothermal biomass graphene and the fungus has the advantages that the additive is prepared by hydrothermal treatment of biochar and graphene, and in combination with the plant arbuscular mycorrhizal fungus, so that the additive has excellent effects on treating novel pollutants in soil, and the biochar and the graphene are fully resourcefully utilized at low cost, and the treatment effect on the novel pollutants in soil is improved.

[0018] The test method fully shows the degradation effect of the additive of the hydrothermal biomass graphene and the fungus on the novel pollutants in soil by comparing the degradation of the novel pollutants in soil by the additive of the hydrothermal biomass graphene and the fungus with the degradation of the novel pollutants in soil by the hydrothermal biomass graphene and the fungus respectively. BRIEF DESCRIPTION OF DRAWINGS

[0019] Figure 1 The figure is a step diagram of the additive preparation method of the hydrothermal biomass graphene and the fungus according to the embodiment of the application;

[0020] Figure 2 The figure is a degradation data diagram of PPCPs in the test method according to the embodiment of the application. DETAILED DESCRIPTION

[0021] In order to make the objects, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application will be described clearly and completely below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only some but not all of the embodiments of the present application. The components of the embodiments of the present application described and shown in the drawings herein can be arranged and designed in various different configurations.

[0022] Therefore, the following detailed description of the embodiments of the present application provided in the drawings is not intended to limit the scope of the claimed present application, but only represents selected embodiments of the present application. All other embodiments obtained by those of ordinary skill in the art based on the embodiments in the present application without creative work fall within the scope of protection of the present application.

[0023] It should be noted that: similar reference numerals and letters indicate similar items in the following drawings, therefore, once an item is defined in one drawing, it does not need to be further defined and explained in subsequent drawings.

[0024] In the description of the embodiments of the present application, it should be understood that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, or the orientation or positional relationship commonly understood by those skilled in the art, and are only intended to facilitate the description of the present application and simplify the description, and are not intended to indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as a limitation on the present application.

[0025] In the description of the embodiments of the present application, it should also be noted that unless otherwise explicitly specified and limited, the terms "set", "mount", "connected", "connected" should be understood broadly, for example, it can be fixedly connected, or it can be detachably connected, or integrally connected; it can be directly connected, or indirectly connected through an intermediate medium, or it can be the communication between the two elements. For those of ordinary skill in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0026] It should be noted that the embodiments in the present application and the features in the embodiments can be combined with each other without conflict.

[0027] The technical solutions in the present application will be described below with reference to the drawings.

[0028] Reference should be made to Figure 1The embodiment of the application provides a kind of hydrothermal biomass graphene synergistic additive preparation method of fungus.The method is by hydrothermal treatment to biochar and graphene, and combine with plant cluster arbuscular mycorrhizal fungi, form the additive with excellent effect of governing new type of soil pollutants, realize the full resource utilization of biochar and graphene at low cost, improve the management effect of new type of soil pollutants.

[0029] The method comprises the following steps:

[0030] S1: obtaining straw and manure, and preparing a biochar mixture.

[0031] In the preparation of the biochar mixture, the mass ratio of straw to manure is in the range of 50% to 60% for straw and 40% to 50% for manure. The straw and manure can be fully utilized in this step, achieving the effect of reducing environmental pollution.

[0032] The following examples demonstrate different preparation methods with different proportions:

[0033] Example 1:

[0034] Straw mass ratio: 50%, livestock and poultry manure mass ratio: 50%, prepare a biochar mixture.

[0035] Example 2:

[0036] Straw mass ratio: 60%, livestock and poultry manure mass ratio: 40%, prepare a biochar mixture

[0037] Example 3:

[0038] Straw mass ratio: 55%, livestock and poultry manure mass ratio: 45%, prepare a biochar mixture

[0039] S2: obtaining a biochar mixture and graphene particles, and generating hydrothermal biomass graphene through hydrothermal reaction.

[0040] In this step, the mass ratio of biochar in the biochar mixture is in the range of 5% to 8%, and the mass ratio of graphene in the graphene particles is in the range of 5% to 10%. The temperature range of the hydrothermal reaction is 500°C to 600°C.

[0041] S3: using M. mosei to prepare a plant cluster arbuscular mycorrhizal fungus solution.

[0042] Using M. mosei to prepare a plant cluster arbuscular mycorrhizal fungus solution includes: preparing a glucose solution and cooling it to room temperature; mixing soil cultured with M. mosei and leek root hairs and placing them in the glucose solution for shaking and centrifugation, and screening and rinsing the shaken solution to obtain plant cluster arbuscular mycorrhizal fungi; counting and picking out the plant cluster arbuscular mycorrhizal fungi, and mixing and configuring a plant cluster arbuscular mycorrhizal fungus solution using a low-phosphorus nutrient solution.

[0043] The concentration of glucose solution is 450 g / L; the weight of soil for cultivating M. globosa is in the range of 8 g to 12 g; the volume of glucose solution for oscillation centrifugation is in the range of 45 ml to 60 ml; the oscillation centrifugation time is in the range of 4 min to 5 min; and the mass ratio of plant arbuscular mycorrhizal fungi in the plant arbuscular mycorrhizal fungi solution is in the range of 2% to 3%.

[0044] S4: mixing the hydrothermal biomass graphene with the plant arbuscular mycorrhizal fungi solution to form an additive.

[0045] The additive can be prepared in various forms, and in this embodiment, the mixing in the soil is used as the preparation form. That is, the mixing method of the hydrothermal biomass graphene and the plant arbuscular mycorrhizal fungi solution is as follows: the hydrothermal biomass graphene is mixed with the soil at a mass ratio in the range of 1:4 to 1:6 to form a mixture; and the plant arbuscular mycorrhizal fungi solution is periodically added to the mixture.

[0046] This embodiment also provides a test method. The test method compares the degradation of the additive of the hydrothermal biomass graphene and the plant arbuscular mycorrhizal fungi to the degradation of the hydrothermal biomass graphene and the plant arbuscular mycorrhizal fungi to soil emerging pollutants, so as to fully show the degradation effect of the additive of the hydrothermal biomass graphene and the plant arbuscular mycorrhizal fungi on soil emerging pollutants.

[0047] The test method includes obtaining a first comparison object, i.e., the hydrothermal biomass graphene; obtaining a second comparison object, i.e., the plant arbuscular mycorrhizal fungi solution; mixing the additive with the test soil, and planting three pots of alfalfa and three pots of kaya; mixing the first comparison object with the test soil, and planting three pots of alfalfa and three pots of kaya; mixing the second comparison object with the test soil, and planting three pots of alfalfa and three pots of kaya; measuring the biomass of the alfalfa and the kaya during the planting period, and comparing the content of PPCPs and the transfer amount of PPCPs; and measuring the composition of the test soil during the planting period, and comparing the content of PPCPs.

[0048] The test soil is according to the 1998 Chinese soil classification system, and the test soil is the surface soil of a certain state-owned health product factory in Xinjiang, the surface soil of a certain health product factory in Sichuan, and the surface soil of a certain health product factory in Guangdong.

[0049] The test results are as follows:

[0050] The hydrothermal biomass graphene is more beneficial to improve the buffering performance of soil and increase the soil nutrient content, compared with the treatment of adding only the plant arbuscular mycorrhizal fungi, the soil surface soil of a state-owned health product factory in Xinjiang is increased by 6.2% and 8.4% respectively. The soil surface soil of a health product factory in Sichuan is increased by 4.8% and 6.8% respectively. The soil surface soil around a health product factory in Guangdong is increased by 6.2% and 8.3% respectively. And the total nitrogen content of the three kinds of soil is increased by 7-10%, 6-9% and 9-12% respectively.

[0051] The addition of hydrothermal biomass graphene is more beneficial to promote plant growth, compared with the treatment of adding only the plant arbuscular mycorrhizal fungi, the biomass of butterbur in the soil surface soil of a health product factory in Sichuan and the soil surface soil around a health product factory in Guangdong is increased by 10-13%, and the chlorophyll content of butterbur planted in the last two kinds of soil is increased by 4-6% and 4-7% respectively. The addition of hydrothermal biomass graphene also makes the amount of PPCPs transferred from the soil system to the plant obviously reduced by 6-11% compared with the treatment of adding only the plant arbuscular mycorrhizal fungi, and the accumulation amount of PPCPs in butterbur in the treatment of hydrothermal biomass graphene and plant arbuscular mycorrhizal fungi is reduced by 17%-22% compared with the corresponding treatment of adding only the plant arbuscular mycorrhizal fungi. Therefore, the hydrothermal biomass graphene as the conditioner of the plant arbuscular mycorrhizal fungi can not only further improve the soil properties and promote plant growth, but also effectively limit the migration of PPCPs in the soil-plant system and reduce the potential pollution risk.

[0052] Reference Figure 2 The biomass of alfalfa in the soil surface soil of a state-owned health product factory in Xinjiang is increased by 8-13% in the treatment of hydrothermal biomass graphene and plant arbuscular mycorrhizal fungi, and the chlorophyll content of alfalfa is increased by 4-6%. The addition of hydrothermal biomass graphene also makes the amount of PPCPs transferred from the soil system to the plant obviously reduced by 4-9% compared with the treatment of adding only the plant arbuscular mycorrhizal fungi, and the accumulation amount of PPCPs in alfalfa in the treatment of hydrothermal biomass graphene is reduced by 10%-17% compared with the corresponding treatment of adding only the plant arbuscular mycorrhizal fungi.

[0053] In summary, the main effective effects of the embodiments provided by the application are:

[0054] The preparation method of the hydrothermal biomass graphene and the additive of the fungus has the advantages that the additive with excellent effect of treating new soil pollutants is formed by hydrothermal treatment of biochar and graphene in combination with plant arbuscular mycorrhizal fungi, the biochar and graphene are fully resource utilization at low cost, and the treatment effect of new soil pollutants is improved.

[0055] The test method fully shows the degradation effect of the hydrothermal biomass graphene additive in cooperation with the plant arbuscular mycorrhizal fungi on the soil new pollutants by comparing the degradation of the hydrothermal biomass graphene additive in cooperation with the plant arbuscular mycorrhizal fungi with the hydrothermal biomass graphene and the plant arbuscular mycorrhizal fungi respectively on the soil new pollutants.

[0056] The technical features of the above embodiments can be combined in any manner, and to make the description concise, all possible combinations of the technical features in the above embodiments are not described, however, as long as the combinations of the technical features do not exist contradictions, they should be considered as the scope of the description.

[0057] The above embodiments only express several implementation manners of the present application, the description is more specific and detailed, but it should not be understood as the limitation of the patent scope of the present application. It should be pointed out that for ordinary skilled in the art, several modifications and improvements can be made without departing from the concept of the present application, and these all belong to the protection scope of the present application. Therefore, the protection scope of the patent of the present application should be subject to the appended claims.

Claims

1. A method for preparing an additive of hydrothermal biomass graphene synergized with fungi, characterized in that, The method comprises the following steps: Obtaining straw and manure to prepare a biochar mixture; Obtaining the biochar mixture and graphene particles to generate hydrothermal biomass graphene through a hydrothermal reaction; Using Glomus mosseae to prepare a plant arbuscular mycorrhizal fungus liquid; Mixing the hydrothermal biomass graphene and the plant arbuscular mycorrhizal fungus liquid to form an additive; In the preparation of the biochar mixture, the mass ratio of the straw to the manure ranges from 50% to 60% for the straw and from 40% to 50% for the manure; In the preparation of the hydrothermal biomass graphene, the mass proportion of biochar in the biochar mixture ranges from 5% to 8%, the mass proportion of graphene in the graphene particles ranges from 5% to 10%, and the temperature of the hydrothermal reaction ranges from 500 DEG C to 600 DEG C; The method for preparing the plant arbuscular mycorrhizal fungus liquid using Glomus mosseae comprises the following steps: Preparing a glucose solution and cooling it to room temperature; Mixing soil and leek root hairs in which Glomus mosseae is cultured, placing them in the glucose solution, and performing oscillation centrifugation, and screening and rinsing the oscillated solution to obtain plant arbuscular mycorrhizal fungi; Counting and picking out the plant arbuscular mycorrhizal fungi, and mixing and configuring a low-phosphorus nutrient solution to prepare the plant arbuscular mycorrhizal fungus liquid; In the preparation of the plant arbuscular mycorrhizal fungus liquid, the concentration of the glucose solution is 450 g / L, the weight of the soil in which Glomus mosseae is cultured ranges from 8 g to 12 g, the volume of the glucose solution used for oscillation centrifugation ranges from 45 ml to 60 ml, the oscillation centrifugation time ranges from 4 min to 5 min, and the mass proportion of the plant arbuscular mycorrhizal fungi in the plant arbuscular mycorrhizal fungus liquid ranges from 2% to 3%; The mixing method of the hydrothermal biomass graphene and the plant arbuscular mycorrhizal fungus liquid comprises the following steps: Mixing the hydrothermal biomass graphene with soil at a mass ratio ranging from 1:4 to 1:6 to form a mixture; Periodically adding the plant arbuscular mycorrhizal fungus liquid to the mixture.

2. A test method using the additive prepared by the method of any one of claim 1, characterized in that, The method comprises the following steps: Obtaining a first comparison object, i.e., hydrothermal biomass graphene; Obtaining a second comparison object, i.e., a plant arbuscular mycorrhizal fungus liquid; Mixing the additive with test soil, and planting three pots of alfalfa and three pots of Kobresia setchuanensis; Mixing the first comparison object with test soil, and planting three pots of alfalfa and three pots of Kobresia setchuanensis; Mixing the second comparison object with test soil, and planting three pots of alfalfa and three pots of Kobresia setchuanensis; During the planting period, determining the biomass of the alfalfa and the Kobresia setchuanensis, and comparing the content of PPCPs and the transfer amount of PPCPs; During the planting period, determining the composition of the test soil, and comparing the content of PPCPs.

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