A method using Paracoccus wg1 T Method for treating wastewater containing high concentration of inorganic nitrogen

Abstract

The present invention relates to a method for treating a bacterial infection by using Paracoccus wg1 T The invention relates to a method for treating wastewater containing high concentration of inorganic nitrogen, which belongs to the technical field of waste resource utilization and water environment treatment. T The seed liquid after culture is used to treat nitrogen-containing wastewater, and the Paracoccus wg1 of the present invention T , has a very high tolerance to high concentrations of inorganic nitrogen, and has excellent denitrification capabilities under conditions of high concentrations of inorganic nitrogen. Specifically, sodium nitrate is used as the nitrogen source, sodium propionate is used as the carbon source, NO3 ‑ When the ‑N concentration is 6258.85 mg / L‑N, 97.68 mg / L‑N of NO3 can be degraded in 48 hours ‑ -N; ammonium sulfate as nitrogen source, sodium propionate as carbon source, NH4 + When the ‑N concentration is 9326.28 mg / L‑N, 92.28 mg / L‑N of NH4 can be degraded in 48 hours + ‑N. paracoccus wg1 T Biological denitrification treatment of high-concentration inorganic nitrogen wastewater has great application prospects.

Description

Technical Field

[0001] The present invention relates to a method for treating a bacterial infection by using Paracoccus wg1 T The invention discloses a method for treating wastewater containing high-concentration inorganic nitrogen, belonging to the technical field of waste resource utilization and water environment treatment. Background Art

[0002] With the rapid economic growth in recent years, water pollution has become increasingly serious. Excessive nitrogen content (such as nitrate nitrogen and ammonium nitrogen) in wastewater can be toxic to the aquatic environment. The need for efficient, cost-effective, and widely applicable methods to remove nitrogenous waste from water has attracted increasing attention and research.

[0003] At present, physical, chemical and biological methods are mainly used for denitrification. Physical and chemical denitrification methods have the disadvantages of high cost and easy to cause secondary pollution. However, through the participation of microorganisms, inorganic nitrogen is converted into nitrogen gas or intracellular substances to remove nitrogen from the water environment. It has the characteristics of low cost, simple operation, no secondary pollution and good treatment effect. Traditional biological denitrification includes two processes: aerobic nitrification and denitrification. Nitrification refers to the oxidation of ammonia nitrogen into nitrite nitrogen or nitrate nitrogen by autotrophic nitrifying bacteria under aerobic conditions; while denitrification is the process of reducing nitrate nitrogen or nitrite nitrogen to gaseous nitrogen. However, due to the variety of causes of water pollution, wastewater may be rich in various inorganic nitrogen, including nitrate, nitrite, and ammonium salts. The content of each pollutant is also different, which greatly limits biological denitrification.

[0004] Therefore, it is necessary to find denitrifying microorganisms that can be applied to high-concentration inorganic nitrogen wastewater environments, so that they can be used for denitrification treatment of wastewater containing low concentrations of inorganic nitrogen, as well as denitrification treatment of wastewater containing high concentrations of inorganic nitrogen. Chinese patent document CN110655198A (application number 201810694748.4) discloses a method for treating nitrogen-containing wastewater using a heterotrophic nitrification-aerobic denitrification Paracoccus strain. The nitrogen-containing wastewater contains organic sodium and has a C / N ratio of 8 to 150. The method comprises the following steps: inoculating a heterotrophic nitrification-aerobic denitrification Paracoccus seed liquid into ammonia nitrogen wastewater, and oscillating the reaction at a temperature of 25°C to 40°C and a rotation speed of 90rpm to 180rpm to complete the treatment of ammonia nitrogen wastewater. The strain is Paracoccus LJ2, which is deposited in the Guangdong Provincial Microbial Culture Collection Center with a deposit number of GDMCC NO: 60338. Paracoccus LJ2 has an extremely high ability to tolerate high concentrations of organic matter. The maximum ammonia nitrogen concentration tolerated by this strain is 400 mg·L -1 The maximum nitrate nitrogen concentration tolerated is 300 mg·L -1 The best nitrite nitrogen concentration tolerated is 200 mg·L -1, has excellent ability to remove ammonia nitrogen, nitrate nitrogen and nitrite nitrogen under high organic load conditions. However, further search for strains with higher organic matter concentration tolerance is still necessary for the denitrification treatment of wastewater containing high concentrations of inorganic nitrogen. Summary of the Invention

[0005] In view of the shortcomings of the prior art, the present invention provides a method for T A method for treating wastewater containing high concentrations of inorganic nitrogen.

[0006] The technical solutions of the present invention are as follows:

[0007] Paracoccus wg1 T Application in the treatment of wastewater containing high concentration of inorganic nitrogen.

[0008] According to the present invention, preferably, the Paracoccus wg1 T It is deposited in China Center for Type Culture Collection with the accession number CCTCC AB 2019400.

[0009] According to the preferred embodiment of the present invention, the wastewater contains NO3 - -N、NH4 + -N one or two, the NH4 + -N concentration is 50mg / L~9500mg / L, the NO3 - -N concentration is 50mg / L~6500mg / L.

[0010] A method using Paracoccus wg1 T The method for treating wastewater containing high concentration of inorganic nitrogen comprises the following steps:

[0011] (1) Seed solution preparation

[0012] The activated Paracoccus wg1 T Pick a single colony and inoculate it into LB liquid medium, culture it to the logarithmic growth phase, transfer it to aerobic denitrification medium or heterotrophic nitrification medium, culture it to the logarithmic growth phase, and obtain seed liquid;

[0013] (2) The seed solution obtained in step (1) is inoculated into the wastewater containing inorganic nitrogen, and the culture is shaken at a temperature of 35 to 40° C. and a rotation speed of 150 to 200 rpm to complete the treatment of the wastewater.

[0014] According to the present invention, preferably, in step (1), the Paracoccus wg1 T It is deposited in China Center for Type Culture Collection with the accession number CCTCC AB 2019400.

[0015] According to the preferred embodiment of the present invention, the inoculation amount of the bacterial liquid transferred to the aerobic denitrification medium or the heterotrophic nitrification medium in step (1) is 3-15%.

[0016] According to the preferred embodiment of the present invention, the components of the LB liquid culture medium in step (1) are: 10 g / L tryptone, 5 g / L yeast powder, and 10 g / L sodium chloride;

[0017] The components of the aerobic denitrification culture medium are: sodium nitrate 1g / L, disodium hydrogen phosphate 7.9g / L, potassium dihydrogen phosphate 1.5g / L, sodium propionate 3.67g / L, and trace elements 2mL / L;

[0018] The components of the heterotrophic nitrification medium are: 0.7765 g / L ammonium sulfate, 7.9 g / L disodium hydrogen phosphate, 1.5 g / L potassium dihydrogen phosphate, 3.67 g / L sodium propionate, and 2 mL / L trace elements;

[0019] The trace elements are composed of: 50 g / L EDTA-Na, 5.1 g / L manganese chloride, 1.1 g / L ammonium molybdate, 1.6 g / L cobalt chloride, 1.6 g / L copper sulfate, 5.0 g / L ferrous sulfate, 5.5 g / L calcium chloride, and 2.2 g / L zinc sulfate, with a pH of 7.2 to 7.5.

[0020] According to the present invention, preferably, the culture conditions in step (1) are: temperature of 35-40°C, rotation speed of 150-200 rpm; more preferably: temperature of 37°C, rotation speed of 200 rpm.

[0021] According to the preferred embodiment of the present invention, the wastewater in step (2) contains NO3 - -N、NH4 + -N one or two, the NH4 + -N concentration is 50mg / L~9500mg / L, the NO3 - -N concentration is 50mg / L~6500mg / L.

[0022] Preferably, according to the present invention, the inoculation amount of the seed liquid inoculated into the inorganic nitrogen-containing wastewater in step (2) is 3% to 15%.

[0023] According to the preferred embodiment of the present invention, the culture conditions in step (2) are: temperature of 37° C. and rotation speed of 200 rpm.

[0024] Beneficial effects:

[0025] The present invention utilizes Paracoccus wg1 T The seed liquid after culture is used to treat nitrogen-containing wastewater, and the Paracoccus wg1 of the present invention T, has a very high tolerance to high concentrations of inorganic nitrogen, and has excellent denitrification capabilities under conditions of high concentrations of inorganic nitrogen. Specifically, sodium nitrate is used as the nitrogen source, sodium propionate is used as the carbon source, NO3 - When the -N concentration is 6258.85 mg / LN, 97.68 mg / LN of NO3 can be degraded in 48 hours - -N; NO3 - When the -N concentration was 122.83 mg / LN, 119.66 mg / LN of NO3 was degraded in 48 hours. - -N, the denitrification rate is 97.42%. With ammonium sulfate as nitrogen source, sodium propionate as carbon source, NH4 + When the -N concentration is 9326.28 mg / LN, 92.28 mg / LN of NH4 can be degraded in 48 hours + -N; NH4 + When the -N concentration was 113.76 mg / LN, the denitrification rate was 99.16% in 48 hours. T It has the ability to tolerate high concentrations of ammonia nitrogen and high concentrations of nitrate nitrogen, using Paracoccus wg1 T Biological denitrification treatment of high-concentration inorganic nitrogen wastewater has great application prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 Paracoccus wg1 T Denitrification and growth in aerobic denitrification medium.

[0027] Figure 2 Paracoccus wg1 T Denitrification and growth in heterotrophic nitrifying medium. DETAILED DESCRIPTION

[0028] The technical solution of the present invention will be further described below with reference to the examples, but the scope of protection of the present invention is not limited thereto. The reagents and drugs involved in the examples are all common commercial products unless otherwise specified.

[0029] Paracoccus sp. or Paracoccus binzhouense sp. nov. wg1 T It was deposited in the China Center for Type Culture Collection through the non-patented strain preservation channel in 2019, with the preservation number CCTCC AB 2019400.

[0030] The culture medium formula composition involved in the embodiment is:

[0031] LB liquid medium: 10 g / L tryptone, 5 g / L yeast powder, 10 g / L sodium chloride; solid medium supplemented with 20 g / L agar.

[0032] Aerobic denitrification medium: sodium nitrate 1g / L, disodium hydrogen phosphate 7.9g / L, potassium dihydrogen phosphate 1.5g / L, sodium propionate 3.67g / L, trace elements 2mL / L;

[0033] Heterotrophic nitrification medium: ammonium sulfate 0.7765 g / L, disodium hydrogen phosphate 7.9 g / L, potassium dihydrogen phosphate 1.5 g / L, sodium propionate 3.67 g / L, trace elements 2 mL / L;

[0034] The trace elements are: 50 g / L EDTA-Na, 5.1 g / L manganese chloride, 1.1 g / L ammonium molybdate, 1.6 g / L cobalt chloride, 1.6 g / L copper sulfate, 5.0 g / L ferrous sulfate, 5.5 g / L calcium chloride, 2.2 g / L zinc sulfate, and pH = 7.2-7.5.

[0035] The detection methods of ammonia nitrogen, nitrate nitrogen, nitrite nitrogen and total nitrogen (TN) involved in the embodiment are shown in Table 1:

[0036] Table 1. Detection methods for ammonia nitrogen, nitrate nitrogen, nitrite nitrogen and total nitrogen

[0037] Test items Detection method <![CDATA[NH4 + -N]]> Hypobromite oxidation method <![CDATA[NO3 - -N]]> UV spectrophotometry <![CDATA[NO2 - -N]]> Naphthylethylenediamine hydrochloride spectrophotometry TN Alkaline potassium persulfate digestion UV spectrophotometry

[0038] Example 1: Paracoccus wg1 T Activation and denitrification performance testing

[0039] (1) Strain activation

[0040] Take the preserved Paracoccus wg1 T , streak on LB solid medium and culture single colonies at 37°C to obtain activated strains;

[0041] (2) Seed solution preparation

[0042] The Paracoccus wg1 activated in step (1) T A single colony was picked and inoculated into LB liquid medium, cultured at 37°C and 200 rpm with shaking until the logarithmic growth phase, centrifuged to remove the supernatant, washed with sterile water, and resuspended in an equal volume of the bacterial liquid before centrifugation. The inoculum was inoculated into aerobic denitrification medium at a ratio of 5%, cultured at 37°C and 200 rpm with shaking until the logarithmic growth phase, centrifuged to remove the supernatant, and then added with an equal volume of sterile water to obtain the seed solution;

[0043] (3) Initial inspection of denitrification performance

[0044] The seed solution obtained in step (2) was inoculated into aerobic denitrification medium and heterotrophic nitrification medium at an inoculum ratio of 5%, respectively, and cultured at 37°C and 200 rpm with shaking. The changes in the contents of nitrate nitrogen, nitrite nitrogen, and ammonia nitrogen were detected within 28 hours. The results were as follows: Figure 1 and 2 shown.

[0045] The results showed that in aerobic denitrification medium, Paracoccus wg1 T Under the conditions of sodium nitrate as nitrogen source and sodium propionate as carbon source, the strain was in the delayed period of nitrate nitrogen degradation from 0 to 15 hours. The main reason for this phenomenon was that the strain was in the growth retardation period and the number of strains that could degrade nitrate nitrogen was small. After 15 hours, as the strain entered the logarithmic phase, the number of strains increased rapidly and the nitrate nitrogen concentration decreased rapidly. Within 24 hours, NO3 - The -N content dropped from 181 mg / LN to 18.6 mg / LN, the denitrification rate was 89.7%, and the average denitrification rate within 15h to 24h was 16.67 mg / LN / h.

[0046] In heterotrophic nitrification medium, Paracoccus wg1 T Under the conditions of ammonium sulfate as nitrogen source and sodium propionate as carbon source, the strain was in the lag phase of ammonia nitrogen degradation from 0 to 12 hours due to the small number of strains. After 12 hours, as the strain entered the logarithmic phase, the number of strains increased rapidly and the ammonia nitrogen concentration decreased rapidly. Within 28 hours, NH4 + -N decreased from 104mg / LN to 5.4mg / LN, with a denitrification rate of 94.7%. The average denitrification rate within 12h to 24h was 6.25mg / LN / h, and the average denitrification rate within 20h to 24h was 6.17mg / LN / h. At the same time, no accumulation of nitrate nitrogen and nitrite nitrogen was found during the heterotrophic nitrification process, which also shows that Paracoccus wg1 T It has good nitrification and denitrification capabilities.

[0047] Example 2: Paracoccus wg1 T Ammonia nitrogen tolerance test

[0048] (1) Seed solution preparation

[0049] The activated Paracoccus wg1 T A single colony was picked and inoculated into LB liquid medium, cultured at 37°C and 200 rpm with shaking until the logarithmic growth phase, centrifuged to remove the supernatant, washed with sterile water, and resuspended in an equal volume of the bacterial liquid before centrifugation. The culture was inoculated into aerobic denitrification medium at an inoculum ratio of 5%, cultured at 37°C and 200 rpm with shaking until the logarithmic growth phase, centrifuged to remove the supernatant, and then added with an equal volume of sterile water to obtain the seed solution;

[0050] (2) Ammonia nitrogen tolerance test

[0051] In this example, the content of sodium propionate as the carbon source in the heterotrophic nitrification culture medium was fixed, and ammonium sulfate was used as the nitrogen source. The NH4 + -N concentration. NH4 + -N concentrations were set to 100 mg / LN, 1000 mg / LN, 3000 mg / LN, 6000 mg / LN, 8000 mg / LN and 10000 mg / LN, respectively. Three replicates were set for each experiment. Other components and culture conditions of the heterotrophic nitrification medium remained unchanged. The seed solution obtained in step (1) was inoculated into the above-mentioned heterotrophic nitrification medium at an inoculum size of 5%, and cultured at 37°C, 200 rpm, and shaken for 48 h.

[0052] Since only ammonium sulfate is used as nitrogen source in heterotrophic nitrification culture medium, NH4 + The actual content of -N is the actual content of TN. Before and after culture, the actual content of TN in the heterotrophic nitrification medium was measured, and the actual TN degradation was used to represent the degradation of Paracoccus wg1. T NH4 + Degradation of -N. paracoccus wg1 T The degradation of TN at different concentrations is shown in Table 2:

[0053] Table 2. Paracoccus wg1 T Changes in TN content in the culture supernatant after 48 h degradation of different concentrations of TN

[0054]

[0055] Due to errors in the preparation of the culture medium, NH4 + The initial set concentration of -N was different from the actual measured concentration, but from the results in Table 2, we can see that Paracoccus wg1 T In a heterotrophic nitrification medium with ammonium sulfate as the only nitrogen source and a TN concentration as high as 9326.28 mg / LN, 5% Paracoccus wg1 was inoculated. T The seed liquid is still active after 48 hours of growth and has a certain denitrification effect; Paracoccus wg1 T The denitrification performance of Paracoccus wg1 decreased with the increase of TN concentration. When the TN concentration was 113.76 mg / LN, T The TN denitrification rate was as high as 99.16%. When the TN concentration was 1100.77 mg / LN, Paracoccus wg1 T The TN denitrification rate reached 38.51%; when the TN concentration was 3000.00 mg / LN, Paracoccus wg1 TThe TN denitrification rate can still reach 13.35%; when the TN concentration is 9326.28 mg / LN, Paracoccus wg1 T The above results show that Paracoccus wg1 T It can adapt to higher concentrations of ammonia nitrogen and has a degradation effect.

[0056] Example 3: Paracoccus wg1 T Nitrate nitrogen tolerance test

[0057] (1) Seed solution preparation

[0058] The activated Paracoccus wg1 T A single colony was picked and inoculated into LB liquid medium, cultured at 37°C and 200 rpm with shaking until the logarithmic growth phase, centrifuged to remove the supernatant, washed with sterile water, and resuspended in an equal volume of the bacterial liquid before centrifugation. The culture was inoculated into aerobic denitrification medium at an inoculum ratio of 5%, cultured at 37°C and 200 rpm with shaking until the logarithmic growth phase, centrifuged to remove the supernatant, and then added with an equal volume of sterile water to obtain the seed solution;

[0059] (2) Nitrate nitrogen tolerance test

[0060] In this example, the content of sodium propionate as the carbon source in the aerobic denitrification culture medium was fixed, and sodium nitrate was used as the nitrogen source. The NO3 content in the culture medium was changed by changing the content of sodium nitrate. - -N concentration. NO3 - -N concentrations were set to 100 mg / LN, 500 mg / LN, 1000 mg / LN, 3000 mg / LN, and 6000 mg / LN, respectively. Three replicates were set for each experiment. Other components and culture conditions of the aerobic denitrification medium remained unchanged. The seed solution obtained in step (1) was inoculated into the above-mentioned aerobic denitrification medium at a 5% inoculum amount and cultured at 37°C, 200 rpm, and shaking for 48 h.

[0061] Since there is only sodium nitrate as the only nitrogen source in the aerobic denitrification medium, the NO3 - The actual content of -N is the actual content of TN. The actual content of TN in the aerobic denitrification medium before and after culture was measured to show the degradation of TN by Paracoccus wg1. T NO3 - Degradation of -N. paracoccus wg1 T The degradation of TN at different concentrations is shown in Table 3:

[0062] Table 3. Paracoccus wg1 T Changes in TN content in the culture supernatant after 48 h degradation of different concentrations of TN

[0063]

[0064] Due to errors in the preparation of the culture medium, NO3 - There is a difference between the initial set concentration of -N and the actual measured concentration, but from the results in Table 3, it can be seen that Paracoccus wg1 T In an aerobic denitrification medium with sodium nitrate as the sole nitrogen source and a TN concentration as high as 6258.85 mg / LN, 5% Paracoccus wg1 was inoculated. T The seed liquid is still active after 48 hours of growth and has a certain denitrification effect; Paracoccus wg1 T The denitrification performance of Paracoccus wg1 decreased with the increase of TN concentration. When the TN concentration was 122.83 mg / LN, T The TN denitrification rate was as high as 97.42%; when the TN concentration was 545.30 mg / LN, Paracoccus wg1 T The TN denitrification rate reached 68.74%; when the TN concentration was 1030.04 mg / LN, Paracoccus wg1 T The TN denitrification rate can still reach 31.53%; when the TN concentration is 6258.85 mg / LN, Paracoccus wg1 T The above results show that Paracoccus wg1 T It can adapt to higher concentrations of nitrate nitrogen and has a degradation effect.

[0065] Example 4: Paracoccus wg1 T Applied to the treatment of wastewater containing high concentration of inorganic nitrogen

[0066] (1) Preparation of seed solution

[0067] The activated Paracoccus wg1 T A single colony was picked and inoculated into LB liquid medium, cultured at 37°C and 200 rpm with shaking until the logarithmic growth phase, centrifuged to remove the supernatant, washed with sterile water, and resuspended in an equal volume of the bacterial liquid before centrifugation. The culture was inoculated into aerobic denitrification medium at an inoculum ratio of 5%, cultured at 37°C and 200 rpm with shaking until the logarithmic growth phase, centrifuged to remove the supernatant, and then added with an equal volume of sterile water to obtain the seed solution;

[0068] (2) Treatment of simulated wastewater containing high concentrations of inorganic nitrogen

[0069] In this example, aerobic denitrification culture medium was used as the basis for simulated wastewater. The type and content of nitrogen source in the culture medium were changed, that is, the nitrogen source was sodium nitrate, ammonium sulfate, or ammonium sulfate and sodium nitrate, to obtain three different simulated wastewaters containing high concentrations of inorganic nitrogen. The set nitrogen source concentrations were 3000 mg / L NO3 --N, containing 3000mg / L NH4 + -N, containing 1500mg / LNO3 - -N and 1500mg / L NH4 + -N, three replicates were set for each experiment, and other components of the aerobic denitrification medium remained unchanged. The seed solution obtained in step (1) was inoculated into the above-mentioned simulated wastewater at a rate of 5%, and the culture conditions were 37°C, 200rpm, and cultured for 24h.

[0070] The actual TN content in the aerobic denitrification medium before and after cultivation was determined, and the actual TN degradation was used to represent the degradation of Paracoccus wg1. T The treatment capacity of wastewater containing high concentration of inorganic nitrogen. The degradation of TN by Paracoccus in different simulated wastewaters is shown in Table 4:

[0071] Table 4. Paracoccus wg1 T Changes in TN content in the wastewater supernatant after 24h degradation of different simulated wastewater

[0072]

[0073] Due to the error in the preparation of the culture medium, the initial set concentration of the nitrogen source was different from the actual measured concentration. T For NH4 + -N, NO3 - -N high concentration nitrogenous wastewater, has a good tolerance, among which, compared with the other two simulated wastewaters containing a single nitrogen source, under the condition of similar total nitrogen content, Paracoccus wg1 T In the presence of NH4 + -N and NO3 - -N has a higher denitrification effect in the simulated wastewater with two nitrogen sources. The reason is speculated to be that Paracoccus wg1 T The removal of ammonia nitrogen utilizes the heterotrophic ammonium assimilation capacity. Heterotrophic ammonium assimilation is an important driving force for aerobic denitrification. The addition of ammonium can promote the reduction of nitrate, reduce the accumulation of nitrite, promote the removal of TN, and improve the efficiency of aerobic denitrification.

[0074] Therefore, Paracoccus wg1 T It has a high tolerance to inorganic nitrogen and good denitrification performance. The bacterium can be applied to a variety of wastewater treatment environments with different inorganic nitrogen concentrations, and is superior to most of the currently reported Paracoccus denitrification strains.

[0075] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. Paracoccus Paracoccus sp.)wg1 T Application in treating wastewater containing high concentration of inorganic nitrogen; Paracoccus wg1 T It is deposited in China Center for Type Culture Collection with the accession number CCTCC AB 2019400; The wastewater contains NO3 - -N、NH4 + -N one or two, the NH4 + -N concentration is 1100.77mg / L~9500mg / L, the NO3 - -N concentration is 545.30mg / L~6500mg / L.

2. A method using Paracoccus Paracoccus sp.)wg1 T The method for treating wastewater containing high concentration of inorganic nitrogen is characterized by: The steps include: (1) Seed solution preparation The activated Paracoccus wg1 T Pick a single colony and inoculate it into LB liquid medium, culture it to the logarithmic growth phase, transfer it to aerobic denitrification medium or heterotrophic nitrification medium, culture it to the logarithmic growth phase, and obtain seed liquid; the Paracoccus wg1 T It is deposited in China Center for Type Culture Collection with the accession number CCTCC AB 2019400; (2) The seed solution obtained in step (1) is inoculated into the wastewater containing inorganic nitrogen, and the wastewater is shaken and cultured at a temperature of 35-40°C and a rotation speed of 150-200 rpm to complete the treatment of the wastewater; the wastewater contains NO3 - -N、NH4 + -N one or two, the NH4 + -N concentration is 1100.77mg / L~9500mg / L, the NO3 - -N concentration is 545.30mg / L~6500mg / L.

3. The method according to claim 2, wherein The inoculation amount of the bacterial liquid transferred to the aerobic denitrification medium or heterotrophic nitrification medium in step (1) is 3-15%.

4. The method according to claim 2, wherein The components of the LB liquid culture medium in step (1) are: 10 g / L tryptone, 5 g / L yeast powder, and 10 g / L sodium chloride; The components of the aerobic denitrification culture medium are: sodium nitrate 1g / L, disodium hydrogen phosphate 7.9g / L, potassium dihydrogen phosphate 1.5g / L, sodium propionate 3.67g / L, and trace elements 2mL / L; The components of the heterotrophic nitrification medium are: 0.7765 g / L ammonium sulfate, 7.9 g / L disodium hydrogen phosphate, 1.5 g / L potassium dihydrogen phosphate, 3.67 g / L sodium propionate, and 2 mL / L trace elements; The trace element composition is: EDTA-Na 50 g / L, manganese chloride 5.1 g / L, ammonium molybdate 1.1 g / L, cobalt chloride 1.6 g / L, copper sulfate 1.6 g / L, ferrous sulfate 5.0 g / L, calcium chloride 5.5 g / L, zinc sulfate 2.2 g / L, pH=7.2~7.

5.

5. The method according to claim 2, wherein The culture conditions in step (1) are: temperature of 35-40°C and rotation speed of 150-200 rpm.

6. The method according to claim 2, wherein The culture conditions in step (1) are: temperature of 37°C and rotation speed of 200 rpm.

7. The method according to claim 2, wherein The inoculation amount of the seed liquid inoculated into the inorganic nitrogen-containing wastewater in step (2) is 3% to 15%.

8. The method according to claim 2, wherein The culture conditions in step (2) are: temperature of 37°C and rotation speed of 200 rpm.

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