An animal disinfection and sterilization composition and its preparation method

Abstract

The present invention relates to the technical field of bactericides, and in particular to a composition for animal disinfection and sterilization and a preparation method thereof. The disinfection and sterilization composition comprises the following raw materials in parts by weight: 0.3-1 parts of polylysine, 0.05-0.3 parts of natamycin, 0.5-2 parts of nisin, 0.3-1 parts of phenyllactic acid, 0.05-0.15 parts of biotin, 0.1-0.3 parts of catechu extract, 0.0001 parts of linalool, and 90-100 parts of water. The animal disinfection and sterilization composition provided by the present invention is a pure biological formula, which can achieve the purpose of gently and effectively suppressing pathogenic microorganism infection, and has a lasting effect. On the basis of ensuring animal disinfection and sterilization / antibacterial effect, it further ensures safety to humans and animals, and eliminates environmental pollution problems.

Description

Technical field:

[0001] The present invention relates to the technical field of fungicides, in particular to a composition for animal disinfection and sterilization and a preparation method thereof. Background technology:

[0002] With the improvement of people's living standards and the change of people's concepts, more and more people are starting to keep pets, mainly dogs, cats and other animals. However, because these animals carry Microsporum canis, Trichophyton mentagrophytes and other pathogenic microorganisms, these pathogens can easily cause animals to experience symptoms such as hair loss, itching, rashes, and increased purulent or bloody secretions, seriously endangering their health. They can also easily be transmitted to humans when they are close to humans.

[0003] Polylysine, a metabolite produced by Streptomyces, can disrupt the cell membrane structure of microorganisms, causing disruptions in the flow of substances, energy, and information, leading to cell death. Natamycin, a polyolefin macrolide compound produced by Streptomyces natalensis, binds to sterol compounds on the fungal cell membrane through its lactone ring structure, causing cell membrane distortion, leakage, and cell death. Nisin, a metabolite of Streptococcus lactis, acts on bacterial cell membranes, forming pores that disrupt the balance between inside and outside the cell, leading to cell death. Phenyllactic acid, a small molecule organic acid, can disrupt cell membrane integrity, leading to the leakage of cellular contents such as proteins and nucleic acids. It can also interfere with DNA synthesis and affect protein synthesis, ultimately killing microorganisms. These bioactive substances are commonly used in food preservation, but their use in animal disinfection and sterilization has rarely been reported.

[0004] Existing animal disinfectants are mostly made with hypochlorous acid as the main ingredient. Hypochlorous acid emits a very pungent, chlorine-like odor during spraying and is extremely unstable. It also has a strong bleaching effect on clothing, furniture, and other items. In particular, if hypochlorous acid enters an animal's mouth or nose, it can cause poisoning. Other animal disinfectants, such as alkaline compounds, phenolic compounds, and quaternary ammonium compounds, are formulated to kill harmful microorganisms such as bacteria, fungi, and viruses. These products often have a certain degree of irritation, causing varying degrees of irritation or damage to human or animal skin and body tissue. These chemical disinfectants also pose varying degrees of environmental pollution during their production.

[0005] The use of biological antibacterial agents such as polylysine, natamycin, nisin, and phenyllactic acid can effectively inhibit the growth and reproduction of most pathogenic microorganisms in animals, effectively disinfect and sterilize them, reduce the probability of illness in humans and animals, and reduce toxicity to humans and animals and environmental pollution.

[0006] Therefore, this application further optimizes the formula based on the broad-spectrum antibacterial effect of compounding polylysine, natamycin, nisin, phenyllactic acid and other biological antibacterial agents to obtain a safe and effective animal disinfection and sterilization biological spray. Summary of the invention:

[0007] In order to solve the above technical problems, the present invention provides a biological spray for animal disinfection and sterilization. On the basis of polylysine, natamycin, nisin, and phenyllactic acid, biotin, catechu extract, and linalool are added to greatly improve the antibacterial properties, gently and effectively kill pathogenic microorganisms carried by animals, with a long-lasting effect, and promote wound healing and animal hair regrowth.

[0008] One of the technical solutions provided by the present invention is a disinfectant and sterilization composition, comprising the following raw materials in parts by weight: 0.3-1 parts of polylysine, 0.05-0.3 parts of natamycin, 0.5-2 parts of nisin, 0.3-1 parts of phenyllactic acid, 0.05-0.15 parts of biotin, 0.1-0.3 parts of catechu extract, 0.0001 parts of linalool, and 90-100 parts of water;

[0009] Furthermore, the preparation method of the catechu extract is as follows: adding 1 part of a catechu raw material consisting of catechu branches and stems to 10-20 times the weight of a 30-60% ethanol aqueous solution, soaking for 1-2 hours, heating and refluxing under normal pressure for 2-4 times, each time for 1-3 hours, cooling and filtering, and concentrating under reduced pressure at 50-60° C. to 1-2 times the weight of the catechu raw material to obtain the catechu extract;

[0010] Preferably, the composition comprises: 0.5 parts of polylysine, 0.1 parts of natamycin, 1.2 parts of nisin, 0.4 parts of phenyllactic acid, 0.1 parts of biotin, 0.1 parts of catechu extract, 0.0001 parts of linalool, and 90-100 parts of water.

[0011] The second technical solution provided by the present invention is the application of the disinfection and sterilization composition described in the first technical solution, especially in the disinfection and sterilization of animals;

[0012] Furthermore, the application method is spraying or painting;

[0013] Furthermore, the disinfectant composition is suitable for treating diseases caused by microorganisms such as Microsporum canis, Microsporum gypseum, Salmonella, Staphylococcus aureus, and the like, such as skin diseases, and is suitable for common animals such as cats and dogs;

[0014] The third technical solution provided by the present invention is a method for preparing the disinfection and sterilization composition described in the first technical solution, which is specifically as follows:

[0015] At room temperature, polylysine, natamycin, nisin, phenyllactic acid, and biotin were added in proportion, and part of the water was added and stirred thoroughly until dissolved. Then, catechu extract and linalool were added in sequence, and the remaining water was added and stirred again until all contents were completely dissolved. The mixture was then filtered through a 0.22 μm filter membrane, and the pH was adjusted to 3-6 with citric acid.

[0016] Furthermore, the above composition is diluted 1-30 times and then dispensed into sprayable containers to prepare a spray.

[0017] The fourth technical solution provided by the present invention is an animal disinfection and sterilization biological spray prepared by the above method.

[0018] Beneficial effects:

[0019] 1. The animal disinfection and sterilization composition provided by the present invention is a pure biological formula that can achieve the purpose of gently and effectively inhibiting infection by pathogenic microorganisms with a long-lasting effect. On the basis of ensuring the disinfection and sterilization / bacteriostasis effect on animals, it further ensures the safety of humans and animals and eliminates environmental pollution problems.

[0020] 2. The animal disinfection and sterilization composition provided by the present invention can not only inhibit the infection of pathogenic microorganisms at the wounds on the animal skin epidermis, but also promote wound healing and regrowth of animal hair.

[0021] 3. The animal disinfection and sterilization composition provided by the present invention has biotin added to the formula, which greatly improves the sterilization rate of the compounded product; the catechu extract added to the formula not only enhances the overall sterilization effect but also enhances its healing effect on animal skin; the linalool added to the formula not only enhances the overall sterilization effect but also effectively keeps animals quiet, preventing them from licking their wounds, which is conducive to rapid wound healing.

[0022] 4. The animal disinfection and sterilization composition provided by the present invention has a reasonable formula, is widely used, has a relatively low manufacturing cost, is easy to configure, and is easy to use. The product has stable quality during the shelf life, can be stored and transported at room temperature and pressure, is easy to carry, is safe, non-toxic, and environmentally friendly. Specific implementation method:

[0023] The following will be a clear and complete description of the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

[0024] In the present invention and examples, the bactericidal rate is determined according to the bactericidal performance test method in the "Antimicrobial and Antibacterial Effect Evaluation Method WS / T 650-2019". The specific method is as follows:

[0025] Take the test bacteria (Staphylococcus aureus, Salmonella, Microsporum canis) 24h fresh slant culture and wash it with PBS, dilute it with PBS to about 5.0×10 5 CFU / mL~4.5×10 6 CFU / mL bacterial suspension is ready for use. Take a sterile test tube, dilute the test sample with water to 2mL / L, 4mL / L, 6mL / L, 8mL / L, and 10mL / L, add 5.0mL of sample diluent respectively, place in a 20℃ water bath for 5 minutes, then add 0.1mL of test bacterial suspension, mix quickly and start timing immediately. Wait for the test bacteria to interact with the sample for 30 minutes, aspirate 1.0mL of the test bacteria and sample mixture to inoculate 2 plates, and pour the culture medium. When the amount of bacteria cannot be counted, make a 10-fold serial dilution with PBS, select the appropriate dilution and aspirate 1.0mL to inoculate 2 plates for live bacterial culture counting. At the same time, use PBS instead of the sample to conduct parallel experiments as a positive control. The number of colonies recovered in the positive control is 1.0×10 4 CFU / mL~9.0×10 4 CFU / mL. PBS and culture medium from the same batch were used as negative controls. Culture was performed according to the growth requirements of the experimental bacteria (Staphylococcus aureus and Salmonella were cultured at 36°C ± 1°C, and Microsporum canis was cultured at 27°C ± 1°C). Staphylococcus aureus and Salmonella were cultured for 48 hours and the final results were observed; Microsporum canis was cultured for 120 hours and the final results were observed. The test was repeated three times, and the inhibition rate was calculated.

[0026]

[0027] Where:

[0028] X—inhibition rate, %;

[0029] A0—the amount of bacteria recovered in the positive control group, in CFU / mL;

[0030] A1—The amount of bacteria recovered from the experimental group, in CFU / mL.

[0031] The present invention is further explained below through specific examples.

[0032] Example 1: A disinfection and sterilization composition and its preparation

[0033] Preparation of spray formula 1: Under room temperature conditions, add 3g of polylysine, 2g of natamycin, 10g of nisin, and 4g of phenyllactic acid to purified water, stir thoroughly until dissolved, adjust the volume to 1L, and then filter with a 0.22μm filter membrane. Adjust the pH to 5.0 with citric acid.

[0034] After diluting the product prepared by the above method by a certain multiple, the sterilization rate was measured. The results are as follows:

[0035] Sterilization test results of formula 1

[0036] Dilution concentration Staphylococcus aureus salmonella Microsporum canis 10mL / L 99％ 99％ 99％ 8mL / L 99％ 89％ 99％ 6mL / L 69％ 58％ 99％ 4mL / L 26％ 22％ 78％ 2mL / L 13％ 10％ 37％

[0037] Example 2: A disinfectant and sterilization composition and its preparation

[0038] Preparation of spray formula 2: Under room temperature conditions, add 3g of polylysine, 2g of natamycin, 10g of nisin, 4g of phenyllactic acid, and 1g of biotin to purified water, stir thoroughly until dissolved, and adjust the volume to 1L. Then filter with a 0.22μm filter membrane and adjust the pH to 5.0 with citric acid.

[0039] After diluting the product prepared by the above method by a certain multiple, the sterilization rate was measured. The results are as follows:

[0040] Sterilization test results of formula 2

[0041] Dilution concentration Staphylococcus aureus salmonella Microsporum canis 10mL / L 99％ 99％ 99％ 8mL / L 99％ 99％ 99％ 6mL / L 99％ 99％ 99％ 4mL / L 99％ 95％ 99％ 2mL / L 76％ 66％ 91％

[0042] Example 3: A disinfection and sterilization composition and its preparation

[0043] 10 g of catechu branches and stems were added to 150 mL of 40% ethanol aqueous solution, soaked for 2 h, heated under reflux extraction at normal pressure for 3 times, each time for 1 h, cooled and filtered, and concentrated under reduced pressure at 50° C. to 10 g to obtain 10 g of catechu extract.

[0044] Preparation of spray formula three: Under room temperature conditions, add 3g of polylysine, 2g of natamycin, 10g of nisin, and 4g of phenyllactic acid to purified water in sequence, stir thoroughly until dissolved, add 2g of pretreated catechu extract, add water again and stir until all contents are completely dissolved, adjust the volume to 1L, and then filter with a 0.22μm filter membrane. Adjust the pH to 5.0 with citric acid.

[0045] After diluting the product prepared by the above method by a certain multiple, the sterilization rate was measured. The results are as follows:

[0046] Formula three sterilization experiment

[0047] Dilution concentration Staphylococcus aureus salmonella Microsporum canis 10mL / L 99％ 99％ 99％ 8mL / L 99％ 99％ 99％ 6mL / L 90％ 87％ 99％ 4mL / L 59％ 55％ 95％ 2mL / L 38％ 26％ 65％

[0048] Example 4: A disinfection and sterilization composition and its preparation

[0049] 10 g of catechu branches and stems were added to 150 mL of 40% ethanol aqueous solution, soaked for 2 h, heated under reflux extraction at normal pressure for 3 times, each time for 1 h, cooled and filtered, and concentrated under reduced pressure at 50° C. to 10 g to obtain 10 g of catechu extract.

[0050] Preparation of spray formula 4: Under room temperature conditions, add 3g of polylysine, 2g of natamycin, 10g of nisin, 4g of phenyllactic acid, and 1g of biotin to purified water in sequence, stir thoroughly until dissolved, add 2g of pretreated catechu extract, add water again and stir until all contents are completely dissolved, adjust the volume to 1L, then filter with a 0.22μm filter membrane, and adjust the pH to 5.0 with citric acid.

[0051] After diluting the product prepared by the above method by a certain multiple, the sterilization rate was measured. The results are as follows:

[0052] Results of sterilization test of formula 4

[0053] Dilution concentration Staphylococcus aureus salmonella Microsporum canis 10mL / L 99％ 99％ 99％ 8mL / L 99％ 99％ 99％ 6mL / L 99％ 99％ 99％ 4mL / L 99％ 98％ 99％ 2mL / L 81％ 74％ 98％

[0054] Example 5: A disinfection and sterilization composition and its preparation

[0055] 10 g of catechu branches and stems were added to 150 mL of 40% ethanol aqueous solution, soaked for 2 h, heated under reflux extraction at normal pressure for 3 times, each time for 1 h, cooled and filtered, and concentrated under reduced pressure at 50° C. to 10 g to obtain 10 g of catechu extract.

[0056] Preparation of spray formula 5: Under room temperature conditions, add 3 g of polylysine, 2 g of natamycin, 10 g of nisin, 4 g of phenyllactic acid, and 1 g of biotin to purified water in sequence and stir thoroughly until dissolved. Then add 2 g of pretreated catechu extract and 0.001 g of linalool. Add water again and stir until all contents are completely dissolved. The volume is adjusted to 1 L, and then filtered through a 0.22 μm filter membrane. The pH value is adjusted to 5.0 with citric acid.

[0057] After diluting the product prepared by the above method by a certain multiple, the sterilization rate was measured. The results are as follows:

[0058] Results of sterilization test of formula five

[0059] Dilution concentration Staphylococcus aureus salmonella Microsporum canis 10mL / L 99％ 99％ 99％ 8mL / L 99％ 99％ 99％ 6mL / L 99％ 99％ 99％ 4mL / L 99％ 99％ 99％ 2mL / L 88％ 87％ 99％

[0060] Example 6 Animal Experiment

[0061] Animal experiments were conducted using the disinfection and sterilization composition products prepared in Examples 1-5, as follows:

[0062] Fifteen short-haired cats suspected of having skin diseases (specifically manifested by local hair loss, dandruff, circular ringworm spots, redness, scabs, etc.) were found in a pet hospital. These cats were first given a simple cleansing, and the hair on the wounds was shaved with a shaver. Then, they were placed in different cages. The disinfectant and sterilization product to be tested was diluted 100 times and sprayed on these cats and their living environment. The spraying was done once a day, with 10 mL sprayed on the cats and the environment respectively (10 mL of spraying volume can completely cover all parts of the test cats). The reactions of the test cats were observed, and the hair growth and wound healing conditions were recorded.

[0063] Effects of different animal disinfectants on wound healing and hair growth

[0064]

[0065] Note: 1. Hair length refers to the hair length near the wound measured after one week of treatment on cats;

[0066] 2. The criteria for judging wound healing are: Subscab healing

[0067] The animal experiments described above demonstrate that the addition of biotin, catechu extract, and linalool can all enhance the bactericidal / inhibitory effects to a certain extent. Furthermore, the addition of catechu extract not only enhances the overall bactericidal effect but also its healing effect on animal skin. Based on the bactericidal results, Formula 2, which includes biotin, has a higher antibacterial rate than Formula 3, which includes catechu extract. However, the healing time for Formula 3 is shorter. Therefore, it is concluded that catechu extract itself not only has an antibacterial effect but also promotes wound healing. The addition of linalool, while enhancing the overall bactericidal effect, effectively keeps the animals quiet, preventing them from licking their wounds and promoting rapid wound healing.

[0068] Example 7 A disinfection and sterilization composition and its preparation

[0069] A disinfection and sterilization composition comprising the following components: 0.5 parts of polylysine, 0.1 parts of natamycin, 1.2 parts of nisin, 0.4 parts of phenyllactic acid, 0.1 parts of biotin, 0.1 parts of catechu extract, 0.0001 parts of linalool, and 97 parts of water;

[0070] The catechu extract is prepared as follows: adding one part of a catechu raw material consisting of catechu branches and stems to 15 times the weight of a 30% ethanol aqueous solution, soaking for 2 hours, heating and refluxing under normal pressure for extraction three times, each time for 2 hours, cooling and filtering, and concentrating under reduced pressure at 55° C. to 1 times the weight of the catechu raw material to obtain the catechu extract;

[0071] The preparation method of the disinfection and sterilization product is as follows: under room temperature conditions, polylysine, natamycin, nisin, phenyllactic acid, and biotin are added in proportion in sequence, part of water is added and stirred thoroughly until dissolved, catechu extract and linalool are then added in sequence, the remaining water is added and stirred again until all contents are completely dissolved, and then filtered with a 0.22 μm filter membrane, and the pH is adjusted to 4 with citric acid; the above composition is diluted 25 times with water and then divided into sprayable containers to prepare a spray.

[0072] Example 8 A disinfection and sterilization composition and its preparation

[0073] A disinfection and sterilization composition comprising the following ingredients: 0.3 parts of polylysine, 0.05 parts of natamycin, 0.5 parts of nisin, 0.5 parts of phenyllactic acid, 0.05 parts of biotin, 0.2 parts of catechu extract, 0.0001 parts of linalool, and 90 parts of water;

[0074] The preparation method of the catechu extract is as follows: adding 1 part of a catechu raw material consisting of catechu branches and stems to 10 times the weight of a 60% ethanol aqueous solution by volume, soaking for 2 hours, heating and refluxing under normal pressure for 4 times, each time for 1 hour, cooling and filtering, and concentrating under reduced pressure at 60° C. to 1 times the weight of the catechu raw material to obtain the catechu extract;

[0075] The preparation method of the disinfection and sterilization product is as follows: under room temperature conditions, polylysine, natamycin, nisin, phenyllactic acid, and biotin are added in proportion in sequence, part of water is added and stirred thoroughly until dissolved, catechu extract and linalool are then added in sequence, the remaining water is added and stirred again until all contents are completely dissolved, and then filtered with a 0.22 μm filter membrane, and the pH is adjusted to 5 with citric acid; the above composition is diluted 20 times with water and then divided into sprayable containers to prepare a spray.

[0076] Example 9 A disinfection and sterilization composition and its preparation

[0077] A disinfection and sterilization composition comprising the following components: 1 part of polylysine, 0.3 part of natamycin, 1.5 parts of nisin, 0.3 part of phenyllactic acid, 0.1 part of biotin, 0.2 part of catechu extract, 0.0001 part of linalool, and 100 parts of water;

[0078] The catechu extract is prepared as follows: adding one part of a catechu raw material consisting of catechu branches and stems to 20 times its weight in a 40% ethanol aqueous solution, soaking for 2 hours, heating and refluxing under normal pressure for extraction twice, each time for 3 hours, cooling and filtering, and concentrating under reduced pressure at 60° C. to twice the weight of the catechu raw material to obtain the catechu extract;

[0079] The preparation method of the disinfection and sterilization product is as follows: under room temperature conditions, polylysine, natamycin, nisin, phenyllactic acid, and biotin are added in proportion in sequence, part of water is added and stirred thoroughly until dissolved, catechu extract and linalool are then added in sequence, the remaining water is added and stirred again until all contents are completely dissolved, and then filtered with a 0.22 μm filter membrane, and the pH is adjusted to 6 with citric acid; the above composition is diluted 30 times with water and then divided into sprayable containers to prepare a spray.

[0080] Although the present invention has been disclosed above in terms of preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art may make various changes, modifications, substitutions and variations in form and details to these embodiments without departing from the spirit and principles of the present invention. The scope of the present invention is defined by the claims and their equivalents.

Claims

1. An animal disinfection and sterilization composition, characterized in that: The invention comprises the following raw materials in parts by weight: 0.3-1 parts of polylysine, 0.05-0.3 parts of natamycin, 0.5-2 parts of nisin, 0.3-1 parts of phenyllactic acid, 0.05-0.15 parts of biotin, 0.1-0.3 parts of catechu extract, 0.0001 parts of linalool and 90-100 parts of water.

2. The animal disinfection and sterilization composition according to claim 1, wherein: The preparation method of the catechu extract is as follows: adding 1 part of a catechu raw material consisting of catechu branches and stems to 10-20 times the weight of a 30-60% ethanol aqueous solution, soaking for 1-2 hours, heating and refluxing under normal pressure for 2-4 times, each time for 1-3 hours, cooling and filtering, and concentrating under reduced pressure at 50-60° C. to 1-2 times the weight of the catechu raw material to obtain the catechu extract.

3. The animal disinfection and sterilization composition according to claim 1, wherein: The composition comprises: 0.5 parts of polylysine, 0.1 parts of natamycin, 1.2 parts of nisin, 0.4 parts of phenyllactic acid, 0.1 parts of biotin, 0.1 parts of catechu extract, 0.0001 parts of linalool, and 90-100 parts of water.

4. The method for preparing the animal disinfection and sterilization composition according to claim 1, characterized in that: The details are as follows: At room temperature, polylysine, natamycin, nisin, phenyllactic acid, and biotin are added in proportion, part of the water is added, and the mixture is thoroughly stirred until dissolved. Then, catechu extract and linalool are added in sequence, and the remaining water is added and stirred again until all contents are completely dissolved. The mixture is then filtered through a 0.22 μm filter membrane, and the pH is adjusted to 3-6 with citric acid.

5. The preparation method according to claim 4, wherein The above composition is diluted 1-30 times and then dispensed into sprayable containers to prepare a spray.

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