Device for detecting infection markers in peripheral blood

Abstract

The application provides a detection device for infection markers in peripheral blood and relates to the field of infection marker detection, and comprises a casing, spaces in the casing are sequentially a first area, a second area, a third area and a fourth area, a lamp group is located in the first area, and a combination part is located in the second area, wherein the lamp group is connected with the combination part, in the application, an enzyme-labeled plate can move in the second area and the third area under the driving of a cylinder, wherein the enzyme-labeled plate coincides with a light receiver and a light emitter only when the enzyme-labeled plate is in the second area, so that the colorimetric value of a sample is detected, when the enzyme-labeled plate enters the third area from the second area, the enzyme-labeled plate enters the third area obliquely downwards, so that air bubbles in the sample are closer to a bottle opening, and components such as a soft plate frame and a reset part are arranged, so that a soft plate in the soft plate frame directly contacts the air bubbles in the sample, and the adverse effects of the air bubbles on detection are eliminated.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of infection marker detection, in particular to a device for detecting infection markers in peripheral blood. BACKGROUND

[0002] In ELISA experiments, a microplate reader is used to compare the color of samples, so as to display the detection results. Therefore, the position and angle of light entering the sample in the microplate reader are controlled, which is a way to control the detection error of the microplate reader. However, according to the working principle of the microplate reader, adding a stop solution to the sample or shaking the sample may generate bubbles in the sample. For bubbles, they will refract light during the process of light penetrating into the sample, which will seriously affect the colorimetric results of the microplate reader. Therefore, using a needle to prick the bubbles can eliminate the adverse effects, but the needle pricking method is only limited to small-scale use. When there are bubbles in most samples, the process of implementing the needle pricking method is necessarily complicated and difficult. Therefore, it is necessary to introduce a microplate reader capable of eliminating bubbles in ELISA experiments to control the detection error. SUMMARY

[0003] The purpose of the present application is to provide a device for detecting infection markers in peripheral blood, so as to solve the technical problem that the needle pricking method is only limited to small-scale use, and when there are bubbles in most samples, the process of implementing the needle pricking method is necessarily complicated and difficult.

[0004] To solve the above technical problems, the following technical solutions are adopted:

[0005] The device for detecting infection markers in peripheral blood comprises:

[0006] A housing, the space in the housing is sequentially a first area, a second area, a third area and a fourth area;

[0007] A lamp group, the lamp group is located in the first area;

[0008] A combination, the combination is located in the second area, wherein the lamp group is connected with the combination, and the light emitted by the lamp group is subdivided into multiple light rays;

[0009] A light emitter, the light emitter is located in the second area, and is used to guide the multiple light rays vertically upward;

[0010] A light receiver, the light receiver is located in the second area and opposite to the light emitter, wherein the light receiver and the light emitter have a range of motion involving the enzyme-labeled plate in the second area and the third area;

[0011] The enzyme-labeled plate is used to support the sample;

[0012] The enzyme-labeled plate coincides with the light receiver and the light emitter only when it is in the second area.

[0013] When the enzyme plate enters the third area from the second area, the enzyme plate enters the third area obliquely downward;

[0014] A soft plate frame is arranged in the third area, and the soft plate frame has resistance in rotation, wherein a detachable soft plate is arranged in the soft plate frame, and the soft plate is always above the enzyme plate;

[0015] A reset member is arranged in the third area and below the enzyme plate, wherein a first slider is arranged in the reset member and in the moving path of the enzyme plate, the first slider has resistance in sliding in the reset member, and in addition, the first slider and the soft plate frame are connected by a soft rod, so that when the enzyme plate entering the third area pushes the first slider to slide relative to the reset member, the soft plate frame is pulled by the soft rod to deflect to the upper side of the enzyme plate, and the soft plate in the soft plate frame pierces the bubbles in the sample.

[0016] Preferably, the combination includes a light guide fiber component and a gas cylinder for driving the enzyme plate to move in the second area and the third area.

[0017] Preferably, the enzyme plate is provided with a first horizontal bar and a second horizontal bar at two ends respectively, wherein the first horizontal bar is hinged at the enzyme plate, and the second horizontal bar is fixed at the enzyme plate.

[0018] The side of the combination opposite to the first horizontal bar is provided with a first groove, and the first groove is used to top the hinged part of the first horizontal bar and the enzyme plate from the side of the first groove after the first horizontal bar completely enters the first groove, so as to constrain the coincidence of the light receiver and the light emitter of the enzyme plate.

[0019] Preferably, the side wall of the third area is provided with a second groove for guiding the oblique movement of the enzyme plate, wherein the end of the second horizontal bar extends into the second groove, and is used to guide the enzyme plate to enter the third area obliquely with the light receiver and the light emitter coinciding.

[0020] Preferably, the third area is further provided with a first spring, and the two ends of the first spring are connected to the side wall of the third area and the soft plate frame respectively, so that when the enzyme plate enters the third area from the second area and pushes the first slider, the soft plate frame is pulled by the soft rod to rotate downward with resistance.

[0021] Preferably, the reset member includes a sleeve arranged in the third area, and the sleeve is below the enzyme plate entering the third area;

[0022] The top of the sleeve is provided with a wire groove for the sliding of the first slider;

[0023] The sleeve is internally provided with a second spring, two ends of the second spring are respectively a second sliding block in sliding fit with the sleeve and a bottom support fixedly connected with the sleeve, the second sliding block is connected with the first sliding block, and the soft rod connected with the first sliding block is used to pull the soft plate frame downward when the enzyme plate enters the third area from the second area and pushes the first sliding block.

[0024] Preferably, the soft rod has no elasticity.

[0025] Preferably, the soft plate frame rotates downward and stops rotating after contacting the second horizontal strip.

[0026] Preferably, the light receiver is in sliding fit with the assembly, and the light receiver is moved to make the sample fall from top to bottom to the enzyme plate when the enzyme plate is in the second area.

[0027] Preferably, the fourth area is provided with a power distribution part connected with the assembly, the light receiver and the lamp group through a wire harness.

[0028] The present application has the following beneficial effects:

[0029] In the present application, the enzyme plate can move in the second area and the third area under the driving of the air cylinder, the enzyme plate coincides with the light receiver and the light emitter when the enzyme plate is in the second area, so that the colorimetric value of the sample is detected, and when the enzyme plate enters the third area from the second area, the enzyme plate enters the third area obliquely downward, so that the air bubbles in the sample are closer to the bottle opening, and the soft plate in the soft plate frame directly contacts the air bubbles in the sample, so that the adverse effects of the air bubbles in the detection are eliminated. BRIEF DESCRIPTION OF DRAWINGS

[0030] Figure 1 It is a structural schematic view of a detection device for an infection marker in peripheral blood;

[0031] Figure 2 It is a structural schematic view of a detection device for an infection marker in peripheral blood; Figure 1

[0032] Figure 3 It is a structural schematic view of a detection device for an infection marker in peripheral blood; Figure 1

[0033] Figure 4 It is a structural schematic view of a detection device for an infection marker in peripheral blood; Figure 3

[0034] Figure 5 It is a sectional view of a reset part;

[0035] ​​​Label: 1, the shell; 2, the lamp group radiator; 3, the power connection part; 4, the power distribution part; 5, the reset part; 51, the sleeve; 52, the bottom support; 53, the second spring; 54, the second sliding block; 6, the soft rod; 7, the first sliding block; 8, the soft plate frame; 9, the first spring; 10, the enzyme label plate; 11, the combination part; 12, the light receiver; 13, the lamp group; 14, the first slot; 15, the light emitter; 16, the first horizontal bar; 17, the air cylinder; 18, the limit rod; 19, the second horizontal bar; 20, the second slot. DETAILED DESCRIPTION

[0036] In order to make the technical means, creative features, purposes and effects of the present application easy to understand, the following further describes the present application in combination with specific embodiments and drawings. However, the following embodiments are only preferred embodiments of the present application, and are not all. Based on the embodiments in the embodiments, other embodiments obtained by those skilled in the art without creative labor are within the protection scope of the present application.

[0037] The specific embodiments of the present application are described below in combination with the drawings.

[0038] Embodiment 1

[0039] In this embodiment, a detection device for an infection marker in peripheral blood is proposed. As shown in Figures 1-5 , the detection device for the infection marker in peripheral blood includes a shell 1. Specifically, the shell 1 is a rectangle as shown in Figure 1 , and the internal space is sequentially a first area, a second area, a third area, and a fourth area.

[0040] Please refer to Figure 1 and Figure 2 , the lamp group 13 is located in the first area, and in addition, the first area also has a lamp group radiator 2. Specifically, the lamp group radiator 2 is opposite to the lamp group 13, and is used for temperature management of the lamp group 13.

[0041] Please refer to Figure 1 and Figure 3 , the combination part 11 combining the optical fiber member and the air cylinder 17 is located in the second area. The optical fiber member in the combination part 11 is connected with the lamp group 13, and subdivides the light emitted by the lamp group 13 into multiple light rays.

[0042] The second area also has a light emitter 15 and a light receiver 12 adjacent to the combination part 11. Specifically, the light emitter 15 is located below the light receiver 12, and is used for guiding the multiple light rays vertically upward to the light receiver 12. For the light receiver 12, it is also Figure 2 completely coincides with the light emitter 15 at the angle shown in the top view;

[0043] The enzyme plate 10 is provided between the light emitter 15 and the light receiver 12, and the enzyme plate 10 supports the sample, so that the light guided by the light emitter 15 transmits through the sample to the light receiver 12, and the colorimetric detection of the sample is realized.

[0044] For the enzyme plate 10, the moving range thereof involves the second area and the third area.

[0045] ① When the enzyme plate 10 is in the second area, the enzyme plate 10 is coincident with the light receiver 12 and the light emitter 15;

[0046] ② When the enzyme plate 10 enters the third area from the second area, the enzyme plate 10 moves obliquely downward into the third area.

[0047] The explanation of ① is as follows: Figure 2 and Figure 4 The first horizontal strip 16 is hinged to the enzyme plate 10, and the second horizontal strip 19 is fixed to the enzyme plate 10.

[0048] Further, the assembly 11 is provided with the first slot 14 on the side opposite to the first horizontal strip 16, and the first slot 14 is used to press the hinged part of the enzyme plate 10 and the first horizontal strip 16 from the side of the first slot 14 after the first horizontal strip 16 completely enters the first slot 14, so as to constrain the enzyme plate 10 to be coincident with the light receiver 12 and the light emitter 15.

[0049] The explanation of ② is as follows: Figure 4 The side wall of the third area is provided with the second slot 20 for guiding the oblique downward movement of the enzyme plate 10, and the two ends of the second horizontal strip 19 are provided with the limiting rod 18, so that the limiting rod 18 and the second slot 20 slide, and then the enzyme plate 10 moves obliquely downward when the cylinder 17 drives the enzyme plate 10 to enter the third area from the second area.

[0050] The main purpose of making the enzyme plate 10 move obliquely downward is to make the sample bottle mouth inclined, so that the bubbles in the sample are closer to the bottle mouth.

[0051] The core of the embodiment is how to eliminate the bubbles in the sample, and for this purpose, the soft plate frame 8 is further provided, and specifically, the soft plate frame 8 is provided with resistance rotation in the third area, wherein the soft plate frame 8 is provided with a detachable soft plate, and the soft plate is always located above the enzyme plate 10.

[0052] As shown in Figure 3 The first spring 9 is provided in the third area, and the two ends of the first spring 9 are connected to the side wall of the third area and the soft plate frame 8, respectively, and when the enzyme plate 10 enters the third area from the second area and pushes the first sliding block 7, the soft plate frame 8 is pulled by the soft rod 6, so that the soft plate frame 8 rotates downward with resistance.

[0053] As Figure 3 shown, a reset member 5 is also provided, specifically, the reset member 5 is located in the third area, and the reset member 5 is below the enzyme-labeled plate 10, wherein the reset member 5 is provided with a first slider 7 located in the moving path of the enzyme-labeled plate 10, and the first slider 7 has a sliding resistance at the reset member 5; the first slider 7 and the soft plate frame 8 are connected by a soft rod 6, when the enzyme-labeled plate 10 entering the third area pushes the first slider 7 to slide relative to the reset member 5, the soft rod 6 pulls the soft plate frame 8 to deflect to the upper side of the enzyme-labeled plate 10, so that the soft plate in the soft plate frame 8 punctures the bubbles in the sample.

[0054] For the reset member 5, as Figure 5 shown, it includes a sleeve 51 provided in the third area, and the sleeve 51 is below the enzyme-labeled plate 10 entering the third area;

[0055] wherein the top of the sleeve 51 has a line groove for the first slider 7 to slide;

[0056] In addition, the sleeve 51 is also provided with a second spring 53, and the two ends of the second spring 53 are respectively a second slider 54 in sliding fit with the sleeve 51, and a bottom support 52 fixedly connected with the sleeve 51, the second slider 54 is connected with the first slider 7, and when the enzyme-labeled plate 10 enters the third area from the second area and pushes the first slider 7, the soft rod 6 connected with the first slider 7 pulls the soft plate frame 8 to rotate downward.

[0057] There are three points in this embodiment that need to be explained:

[0058] ① The soft rod 6 has no elasticity;

[0059] ② The soft plate frame 8 rotates downward and stops rotating after contacting the second horizontal bar 19;

[0060] ③ The soft plate in the soft plate frame 8 has a plurality of puncture parts, which extend into the sample bottle opening when the soft plate frame 8 contacts the second horizontal bar 19, so as to eliminate the bubbles at the bottle opening. The soft plate also has an adsorption function, that is, when the soft plate adsorbs the liquid in the sample in a small range, it can guide the bubbles to move to the soft plate, so as to eliminate the bubbles.

[0061] Please refer to Figure 2 and Figure 3 In this embodiment, the light receiver 12 is in sliding fit with the combination 11, and is used to move the light receiver 12 when the enzyme-labeled plate 10 is only in the second area, so that the sample falls from top to bottom to the enzyme-labeled plate 10.

[0062] Please refer to Figure 1 and Figure 2The fourth area has a power distribution unit 4 connected to the wire harness connection assembly 11, the light receiver 12 and the light group 13. The first area also has a power receiving unit 3 connected to the power distribution unit 4.

[0063] In the present application, unless otherwise explicitly specified and limited, "on" or "under" of a first feature to a second feature can include that the first and second features are in direct contact, or can include that the first and second features are not in direct contact but are in contact through another feature between them. Moreover, "on", "above" and "over" of a first feature to a second feature includes that the first feature is directly above and obliquely above the second feature, or only means that the first feature is higher than the second feature in horizontal height. "Under", "below" and "underneath" of a first feature to a second feature includes that the first feature is directly below and obliquely below the second feature, or only means that the first feature is lower than the second feature in horizontal height.

[0064] The above shows and describes the basic principles, main features and advantages of the present application. It should be understood by those skilled in the art that the present application is not limited by the above examples, and the above examples and descriptions in the specification are only preferred examples of the present application, and are not intended to limit the present application. Without departing from the spirit and scope of the present application, various changes and improvements can be made to the present application, and these changes and improvements all fall within the scope of the claimed present application. The scope of protection of the present application is defined by the appended claims and their equivalents.

Claims

1. A device for detecting infection markers in peripheral blood, characterized in that, include: The casing, and the spaces inside the casing are, in order, the first zone, the second zone, the third zone, and the fourth zone; The light assembly is located in the first zone; The assembly is located in the second zone, where the lamp assembly is connected to the assembly and subdivides the light emitted by the lamp assembly into multiple light rays; A light emitter, located in the second zone and adjacent to the assembly, is used to guide multiple beams of light vertically upward. A light receiver is located in the second region and faces the light emitter, wherein there is an active range between the light receiver and the light emitter involving the second and third regions of the ELISA plate; ELISA plates are used to support samples; When the microplate is only in the second zone, the microplate overlaps with the light receiver and the light emitter; When the microplate moves from the second zone to the third zone, the microplate enters the third zone at an angle downwards. The flexible plate frame is rotated with resistance in the third zone, wherein a removable flexible plate is provided inside the flexible plate frame, and the flexible plate is always located above the ELISA plate. The reset element is located in the third zone and below the ELISA plate. The reset element has a first slider located within the movement path of the ELISA plate. The first slider slides with resistance at the reset element. In addition, the first slider is connected to the flexible plate frame by a flexible rod. When the ELISA plate enters the third zone and pushes the first slider to slide relative to the reset element, the flexible rod pulls the flexible plate frame to deflect it above the ELISA plate, so that the flexible plate in the flexible plate frame punctures the air bubbles in the sample.

2. The detection device for infection markers in peripheral blood according to claim 1, characterized in that: The assembly includes an optical fiber component and a cylinder for driving the microplate to move within the second and third zones.

3. The detection device for infection markers in peripheral blood according to claim 2, characterized in that: The enzyme-labeled plate has a first horizontal bar and a second horizontal bar at both ends, wherein the first horizontal bar is hinged to the enzyme-labeled plate and the second horizontal bar is fixed to the enzyme-labeled plate. The assembly has a first groove on the side facing the first horizontal bar. After the first horizontal bar is fully inserted into the first groove, the first groove side presses against the hinge of the first horizontal bar and the ELISA plate, constraining the light receiver and light emitter of the ELISA plate to overlap.

4. The detection device for infection markers in peripheral blood according to claim 3, characterized in that: The sidewall of the third zone is provided with a second groove for guiding the ELISA plate to move obliquely, wherein the end of the second horizontal bar extends into the second groove to guide the ELISA plate, which coincides with the light receiver and light emitter, to enter the third zone obliquely.

5. The detection device for infection markers in peripheral blood according to claim 1, characterized in that: The third zone also contains a first spring, with its two ends connected to the side wall of the third zone and the flexible plate frame, respectively. When the enzyme-labeled plate enters the third zone from the second zone and pushes the first slider, the flexible rod pulls the flexible plate frame, causing the flexible plate frame to rotate downward with resistance.

6. The detection device for infection markers in peripheral blood according to claim 1, characterized in that: The reset component includes a sleeve located below the ELISA plate that enters the third zone; The top of the sleeve has a groove for sliding the first slider; A second spring is also provided inside the sleeve. The two ends of the second spring are a second slider that slides with the sleeve and a base that is fixedly connected to the sleeve. The second slider is connected to the first slider and is used to pull the soft plate frame downward when the enzyme label plate moves from the second zone to the third zone and pushes the first slider.

7. The detection device for infection markers in peripheral blood according to claim 5 or 6, characterized in that: The flexible rod is not elastic.

8. The detection device for infection markers in peripheral blood according to claim 5 or 6, characterized in that: The downward-spinning flexible plate frame stops rotating after contacting the second crossbar.

9. The detection device for infection markers in peripheral blood according to claim 1, characterized in that: The light receiver slides into the assembly, allowing the light receiver to be moved so that the sample falls from top to bottom onto the ELISA plate when the ELISA plate is only in the second zone.

10. The detection device for infection markers in peripheral blood according to claim 1, characterized in that: The fourth zone contains a power distribution unit that connects the assembly, light receiver, and lamp assembly via wiring harnesses.

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