Method for disinfecting a hatcher box based on vaporized hydrogen peroxide

Abstract

The application discloses a method for disinfecting a hatching box based on vaporized hydrogen peroxide, which comprises the following steps: when the hatching rate reaches 5%, a vaporized hydrogen peroxide disinfection device is started to make the generated vaporized hydrogen peroxide enter the hatching box through the air outlet of the vaporized hydrogen peroxide disinfection device, so that the hydrogen peroxide concentration in the hatching box is 4-10 ppm, and the hydrogen peroxide concentration is kept for 1-20 hours. The interior environment of the hatching box is disinfected by using the vaporized hydrogen peroxide during the hatching process, so that the bacteria and fungus indexes in the hatching box are effectively reduced, the spread of diseases through the hatching poultry is effectively prevented, and excellent disinfection effect is achieved. In addition, the decomposition products of the vaporized hydrogen peroxide are water and oxygen, which are non-toxic and non-residual. Compared with the traditional formaldehyde fumigation disinfection, the method has the advantages of green environmental protection, safety, no pollution, no harm to human body, no adverse effect on the hatched poultry and the growth of the hatched poultry, etc.

Description

TECHNICAL FIELD

[0001] The present application relates to a method for disinfecting a hatching box based on vaporized hydrogen peroxide, belonging to the technical field of hatching. BACKGROUND

[0002] All birds are hatched by fertilized eggs, and the production of large-scale poultry is completed by hatching boxes and hatching boxes under suitable temperature and humidity conditions. A hatching box can hatch 20,000 to 50,000 eggs at a time, and different birds have different hatching times, from a few dozen days to more than 20 days, and finally hatching in a hatching box. During the hatching process, a large number of poultry gather in a small hatching box, and suitable temperature and humidity are good places for bacteria and fungi to breed, and the eggs themselves are easy to carry a large number of pathogenic microorganisms, so if the disinfection is not strict, it is easy to cause early infection of poultry, causing economic losses to breeders.

[0003] The disinfection management of the hatching box is a very important link in the hatching field, and the traditional disinfection method is to use formaldehyde fumigation disinfection, which uses 30ml of formaldehyde per cubic meter of volume, and 15g of potassium permanganate is filled in a container for fumigation disinfection. However, formaldehyde fumigation disinfection has the defects of strong irritating odor, air pollution, and carcinogenic effect on the human body. In addition, if the operator accidentally splashes on the skin or eyes, it will also cause skin or eye burns. SUMMARY

[0004] In view of the above problems existing in the prior art, the purpose of the present application is to provide a method for disinfecting a hatching box based on vaporized hydrogen peroxide.

[0005] In order to achieve the above-mentioned purpose, the present application adopts the following technical solutions:

[0006] A method for disinfecting a hatching box based on vaporized hydrogen peroxide, comprising the following steps:

[0007] a) connecting the box body of the hatching box with the air outlet of the vaporized hydrogen peroxide disinfection device, the box body of the hatching box is provided with a hatching box air outlet and a hatching box air inlet, and the box body of the hatching box is provided with a temperature probe, an electric heating device, a humidity probe, a water tank, a hydrogen peroxide concentration probe, a fan and an egg car, the egg car is provided with a plurality of egg trays, and the vaporized hydrogen peroxide disinfection device, the temperature probe, the electric heating device, the humidity probe, the hydrogen peroxide concentration probe and the fan are signal connected with the control unit;

[0008] b) Transfer the hatching eggs to the egg tray for hatching. When the chicks start pecking at the shells or when the hatching rate reaches 5%, turn on the vaporized hydrogen peroxide sterilization device. The generated vaporized hydrogen peroxide enters the hatching box through the air outlet of the vaporized hydrogen peroxide sterilization device, so that the concentration of hydrogen peroxide in the hatching box reaches 4-10 ppm, and is maintained at this hydrogen peroxide concentration for 1-20 hours.

[0009] In one embodiment, the vaporized hydrogen peroxide disinfection device includes a shell and a disinfectant container filled with hydrogen peroxide solution. The shell is mounted on the lower part of the inner side wall of a hatching box in a wall-mounted manner. The disinfectant container is fixed to the outside of the hatching box. The air outlet of the vaporized hydrogen peroxide disinfection device is located at the top of the shell, and the air inlet of the vaporized hydrogen peroxide disinfection device is located at the bottom of the shell. The shell contains a vaporization chamber that communicates with both the air inlet and outlet of the vaporized hydrogen peroxide disinfection device. An injection pump is located outside the vaporization chamber. A flash evaporation device and a blower located above the flash evaporation device are located inside the vaporization chamber. The flash evaporation device is connected to the disinfectant container via the injection pump. The blower, flash evaporation device, and injection pump are all connected to the control unit via signal. In step b), when the chicks peck at their shells or when the hatching rate reaches 5%, the vaporized hydrogen peroxide disinfection device is activated. The hydrogen peroxide solution in the liquid container is pumped into the flash evaporation device via the injection pump, where it evaporates instantly to form vaporized hydrogen peroxide. The vaporized hydrogen peroxide is then sent from the outlet of the vaporized hydrogen peroxide disinfection device into the hatching box by the blower. The concentration of vaporized hydrogen peroxide in the hatching box is monitored in real time by a hydrogen peroxide concentration probe to ensure that the concentration of vaporized hydrogen peroxide in the hatching box is maintained at 4–10 ppm.

[0010] In a preferred embodiment, the concentration of hydrogen peroxide solution in the disinfectant container is 30%–40%; and the electric heating temperature of the flash evaporation device is 110–160°C.

[0011] In a preferred embodiment, an electrical device is provided inside the housing and outside the vaporization chamber. The blower, flash evaporator, and injection pump are all connected to the electrical device. The electrical device is signal-connected to a control unit. The control unit controls the electrical device to make the injection pump inject hydrogen peroxide solution at a flow rate of 3 to 20 grams per cubic meter of air per hour.

[0012] In a preferred embodiment, an air heating device is provided inside the vaporization chamber, located below the flash evaporation device, and connected to the electrical device. In step b), the hydrogen peroxide solution in the disinfectant container is pumped into the flash evaporation device via an injection pump, where it evaporates instantly to form vaporized hydrogen peroxide. The vaporized hydrogen peroxide is then expelled from the outlet of the vaporized hydrogen peroxide disinfection device into the hatching box by a blower. Simultaneously, air from inside the hatching box enters the vaporization chamber from the inlet of the vaporized hydrogen peroxide disinfection device. The air heated by the air heating device and the vaporized hydrogen peroxide are then expelled together from the outlet of the vaporized hydrogen peroxide disinfection device by the blower and expelled into the hatching box, thus repeating the cycle.

[0013] In one implementation scheme, in step b), the temperature for hatching eggs in the egg tray during hatching is controlled at 36.7℃~37.5℃, and the humidity is controlled at 65%~75%.

[0014] In one implementation scheme, in step b), during the hatching process, the temperature and humidity inside the hatching box are monitored in real time by temperature and humidity probes. The control unit is equipped with a reference temperature and a reference humidity. When the detected temperature is lower than the reference temperature, the electric heating device is activated to heat the emitted heat to increase the temperature inside the hatching box. When the detected humidity is lower than the reference humidity, water is injected into the water tank in a timely manner to increase the humidity inside the hatching box by evaporation of the water in the water tank.

[0015] In one embodiment, a fixed post is vertically installed inside the hatching box, a fan is located above the fixed post and is situated at the center of the hatching box, and an electric heating device is located below the fixed post.

[0016] In one embodiment, the electric heating device is located near the fan.

[0017] Compared with the prior art, the beneficial technical effects of the present invention are as follows:

[0018] This invention achieves disinfection of the internal environment of the hatching box using vaporized hydrogen peroxide during the hatching process. It not only has a good disinfection effect but is also environmentally friendly. Therefore, this invention has significant progress and application value compared with the prior art. Attached Figure Description

[0019] Figure 1 This is a schematic diagram of the structure of a hatching box using the method of disinfecting the hatching box based on vaporized hydrogen peroxide as described in this invention;

[0020] Figure 2 This is a schematic diagram of the vaporized hydrogen peroxide sterilization device provided by the present invention;

[0021] Figure 3 These are pictures of chicks that have been disinfected using the method described in this invention.

[0022] The diagram is labeled as follows: 1. Hatching box; 2. Vaporized hydrogen peroxide disinfection device; 201. Air outlet of vaporized hydrogen peroxide disinfection device; 202. Shell; 203. Disinfectant container; 204. Air inlet of vaporized hydrogen peroxide disinfection device; 205. Vaporization chamber; 206. Injection pump; 207. Flash evaporation device; 208. Air blower; 209. Electrical equipment; 210. Air heating device; 3. Fan; 4. Egg cart; 5. Egg tray; 6. Hydrogen peroxide concentration probe; 7. Temperature probe; 8. Electric heating device; 9. Humidity probe; 10. Water tank; 11. Exhaust pipe; 12. Fixing post. Detailed Implementation

[0023] The technical solution of the present invention will be further described clearly and completely below with reference to the accompanying drawings and embodiments.

[0024] Example

[0025] Please combine Figures 1 to 2 As shown: This invention provides a method for disinfecting a hatching box based on vaporized hydrogen peroxide, comprising the following steps:

[0026] a) Connect the hatching box 1 to the air outlet 201 of the vaporized hydrogen peroxide disinfection device. The hatching box 1 is provided with a hatching air outlet and a hatching air inlet (not shown in the figure). The hatching box 1 is provided with a temperature probe 7, an electric heating device 8, a humidity probe 9, a water tank 10, a hydrogen peroxide concentration probe 6, a fan 3, and an egg cart 4. The egg cart 4 is provided with several egg trays 5. The vaporized hydrogen peroxide disinfection device 2, the temperature probe 7, the electric heating device 8, the humidity probe 9, the hydrogen peroxide concentration probe 6, and the fan 3 are all connected to the control unit (not shown in the figure).

[0027] b) Transfer the hatching eggs to the egg tray for hatching. The temperature during hatching is controlled at 36.7℃~37.5℃ and the humidity is controlled at 65%~75%. When the chicks start pecking at their shells or when the hatching rate reaches 5%, turn on the vaporized hydrogen peroxide disinfection device 2. The generated vaporized hydrogen peroxide enters the hatching box 1 through the air outlet 201 of the vaporized hydrogen peroxide disinfection device, so that the concentration of hydrogen peroxide in the hatching box 1 is 4~10ppm, and this concentration is maintained for 1~20 hours.

[0028] In addition, in step b), during the vaporized hydrogen peroxide disinfection process, indicator bacterial tablets are placed at the corners inside the hatching box 1 and below the egg tray 5, respectively, so as to evaluate the disinfection and sterilization effect by means of the indicator bacterial tablets; multiple (usually at least three, in this embodiment, three) sterilized cardboard boxes for collecting down are evenly placed inside the hatching box 1, so as to measure the total number of microorganisms on the down to evaluate the disinfection effect.

[0029] In this invention, the vaporized hydrogen peroxide disinfection device 2 includes a housing 202 and a disinfectant container 203 containing hydrogen peroxide solution. The housing 202 is mounted on the lower part of the inner side wall of the hatching box 1 in a wall-mounted manner. The disinfectant container 203 is fixed to the outside of the hatching box 1. The air outlet 201 of the vaporized hydrogen peroxide disinfection device is located at the top of the housing 202, and the air inlet 204 of the vaporized hydrogen peroxide disinfection device is located at the lower part of the housing 202. The housing 202 has a vaporization chamber 205 that communicates with both the air inlet 204 and the air outlet 201 of the vaporized hydrogen peroxide disinfection device. An injection pump 206 is provided outside the vaporization chamber 205. A flash evaporation device 207 and a blower 208 located above the flash evaporation device 207 are provided inside the vaporization chamber 205. The flash evaporation device 207 is connected to the disinfectant container 203 through the injection pump 206 (specifically, conduits are connected between the inlet of the injection pump 206 and the disinfectant container 203, and between the outlet of the injection pump 206 and the flash evaporation device 207, thus connecting the flash evaporation device 207 and the disinfectant container 203 through the injection pump 206). The blower 208, the flash evaporation device 207, and the injection pump 206 are all signal-connected to the control unit. Accordingly, in step b), when the chicks are pecking at their shells or when the hatching rate reaches 5%, the vaporized hydrogen peroxide disinfection device 2 is turned on. The hydrogen peroxide solution in the disinfectant container 203 is transported to the flash evaporation device 207 through the injection pump 206. In the flash evaporation device 207, the solution evaporates instantly to form vaporized hydrogen peroxide. The vaporized hydrogen peroxide is then sent out from the air outlet 201 of the vaporized hydrogen peroxide disinfection device into the hatching box 1 under the action of the blower 208. The concentration of vaporized hydrogen peroxide in the hatching box 1 is monitored in real time by the hydrogen peroxide concentration probe 6 to ensure that the concentration of vaporized hydrogen peroxide in the hatching box 1 is maintained at 4-10 ppm.

[0030] In this invention, the housing 202 is installed on the lower part of the inner side wall of the hatching box 1 in a wall-mounted manner. This allows the main body of the vaporized hydrogen peroxide disinfection device 2 to be installed on the lower part of the inner side wall of the hatching box 1 in a wall-mounted manner, thereby reducing the space occupied by the entire device without affecting the use of the hatching box 1.

[0031] In this invention, the disinfectant container 203 is a transparent glass or plastic bottle to facilitate timely observation and replenishment of the hydrogen peroxide solution. In this invention, the concentration of the hydrogen peroxide solution in the disinfectant container is controlled at 30%–40%.

[0032] In this invention, the blower 208, flash evaporator 207, and injection pump 206 can all be based on existing technologies. The flash evaporator 207 is essentially a heating device for hydrogen peroxide solution and can be an open container (the shape of the container is not limited, it can be such as...). Figure 2 The container shown is an inverted trapezoid (but can be any other shape). A heating wire is installed at the bottom of the container. During use, the hydrogen peroxide solution in the disinfectant container 203 is pumped into the flash evaporator 207 via the injection pump 206. It is then heated by the heating wire at the bottom, causing it to evaporate instantly and form vaporized hydrogen peroxide. This vaporized hydrogen peroxide is then sent from the outlet 201 of the vaporized hydrogen peroxide disinfection device via the blower 208 into the hatching box 1. The speed of the blower 208 is controlled by a control unit. By controlling the speed of the blower 208, the rate at which the vaporized hydrogen peroxide diffuses into the hatching box 1 can be adjusted, thereby regulating the concentration of vaporized hydrogen peroxide within the hatching box 1. In this embodiment, the heating temperature of the flash evaporator is controlled between 110℃ and 160℃.

[0033] Furthermore, an electrical device 209 is provided inside the housing 202 and outside the vaporization chamber 205. The air supply fan 208, flash evaporation device 207, and injection pump 206 are all connected to the electrical device 209. The electrical device 209 is signal-connected to the control unit. Thus, the air supply fan 208, flash evaporation device 207, and injection pump 206 are indirectly signal-connected to the control unit through the electrical device 209. The operation of the air supply fan 208, flash evaporation device 207, and injection pump 206 is controlled by the electrical device 209. For example, the speed at which the injection pump 206 delivers hydrogen peroxide solution can be controlled by the electrical device 209, thereby controlling the generation rate and concentration of vaporized hydrogen peroxide. The electrical device 209 is equivalent to the controller of the vaporized hydrogen peroxide disinfection device 2, and the control unit is equivalent to the main controller of the entire integrated machine. The electrical device 209 and the control unit can both utilize existing technology. In this embodiment, the control unit controls the flow rate of hydrogen peroxide solution injected by the injection pump 206 to be 3-20 grams per cubic meter of air per hour.

[0034] Furthermore, an air heating device 210 is installed inside the vaporization chamber 205. The air heating device 210 is located below the flash evaporation device 207 and is connected to the electrical device 209. Thus, the air heating device 210 is indirectly connected to the control unit via the electrical device 209. The air heating device 210 heats the air entering the vaporization chamber 205 from the air inlet 204 of the vaporized hydrogen peroxide sterilizer. The heated air can then better coordinate with the blower 208 to deliver the vaporized hydrogen peroxide from the air outlet 201 of the vaporized hydrogen peroxide sterilizer into the hatching box 1. The air heating device 210 can utilize existing technology; for example, it can be a commercially available air heater. Correspondingly, in step b), the hydrogen peroxide solution in the disinfectant container 203 is transported to the flash evaporation device 207 by the injection pump 206, where it evaporates instantly to form vaporized hydrogen peroxide. The vaporized hydrogen peroxide is then sent out from the air outlet 201 of the vaporized hydrogen peroxide disinfection device into the hatching box 1 by the blower 208. At the same time, air inside the hatching box 1 enters the vaporization chamber 205 from the air inlet 204 of the vaporized hydrogen peroxide disinfection device. The air heated by the air heating device 210 and the vaporized hydrogen peroxide are then sent out from the air outlet 201 of the vaporized hydrogen peroxide disinfection device into the hatching box 1 by the blower 208. This cycle is repeated to maintain the concentration of vaporized hydrogen peroxide in the box at 4-10 ppm.

[0035] In this invention, the blower 208, flash evaporation device 207, and air heating device 210 are all disposed within the vaporization chamber 205, while the injection pump 206 and electrical device 209 are disposed outside the vaporization chamber 205. This arrangement ensures the normal generation of vaporized hydrogen peroxide while isolating the injection pump 206 and electrical device 209 from contact with the vaporized hydrogen peroxide, thus protecting them. Furthermore, the vaporization chamber 205 provides a gas flow channel within the housing 202, eliminating the need for connecting pipelines between the blower 208, flash evaporation device 207, and air heating device 210, thereby simplifying the structure of the vaporized hydrogen peroxide disinfection device 2.

[0036] In this invention, the control unit, temperature probe 7, electric heating device 8, humidity probe 9, water tank 10, hydrogen peroxide concentration probe 6, fan 3, egg cart 4, and egg tray 5 can all be commercially available products. For example, the control unit can be a commercially available controller, such as a microcontroller; the control unit can also be connected to a control terminal (PC, mobile phone, etc.) to achieve remote or mobile control; the electric heating device 8 can be a commercially available heating wire. Accordingly, in step b), during the hatching process, the temperature and humidity inside the hatching box 1 are monitored in real time by the temperature probe 7 and humidity probe 9. The control unit has a reference temperature and a reference humidity. When the detected temperature is lower than the reference temperature, the electric heating device 8 is activated, and the heat emitted after heating by the electric heating device 8 is used to increase the temperature inside the hatching box 1; when the detected humidity is lower than the reference humidity, water is injected into the water tank 10 in time, and the evaporation of water in the water tank 10 is used to increase the humidity inside the hatching box 1.

[0037] During installation, temperature probe 7 and humidity probe 9 can be installed on the top wall of the hatching box 1; hydrogen peroxide concentration probe 6 can be installed on the lower part of the top wall and inner side wall of the hatching box 1 (e.g., Figure 1 (as shown) and the upper part of the inner wall, while needing to be far away from the air outlet of the hatching box. The number of hydrogen peroxide concentration probes 6 can be flexibly adjusted according to the actual situation, usually 1 to 4; the fan 3 is installed in the middle position inside the hatching box 1; the water tank 10 is installed in the middle position at the bottom of the hatching box 1.

[0038] In this embodiment, the fan 3 works in conjunction with the air outlet and air inlet of the hatching box to maintain air circulation inside the hatching box 1. During use, an exhaust pipe 11 is connected to the air outlet of the hatching box (e.g., ...). Figure 1 As shown, the air inside the hatching box 1 is exhausted through the exhaust pipe 11, and air from outside is introduced into the hatching box 1 through the hatching box air inlet. The hatching box air outlet and hatching box air inlet are usually located at the top of the hatching box. While maintaining air circulation inside the hatching box 1, the fan 3 can also work with the electric heating device 8 and the water tank 10 to regulate temperature and humidity, so as to maintain constant temperature and humidity inside the hatching box 1. When the electric heating device 8 and the water tank 10 are working, the fan 3 can distribute the heat emitted by the electric heating device 8 and the evaporation of water in the water tank 10 more quickly and evenly inside the hatching box 1, thus achieving better temperature and humidity regulation.

[0039] In addition, please see Figure 1As shown, a fixing post 12 is vertically installed inside the hatching box 1, and the fan 3 is located on top of the fixing post 12, with the entire fan positioned at the center inside the hatching box 1. The fan 3 is fixed inside the hatching box 1 by the fixing post 12. In addition, circuitry can be arranged inside the fixing post 12 to provide power to the fan 3.

[0040] In this invention, the electric heating device 8 is located near (around) the fan 3, and the number of electric heating devices 8 can be one (e.g., Figure 1 (As shown) or more, can be installed to the side, above, or below the fan 3 so that the fan 3 can disperse the heat generated by the electric heating device 8. During installation, the electric heating device 8 can be installed at the lower part of the fixed pile 12 (e.g., as shown). Figure 1 (as shown), so that it is located below the fan 3, or it can be installed on the side or above the fan 3 by another fixed bracket.

[0041] The following experiments compare the method for disinfecting hatching boxes based on vaporized hydrogen peroxide described in this invention with the traditional formaldehyde fumigation disinfection method.

[0042] 1. Materials and Methods

[0043] 1.1 Test Materials

[0044] 1.1.1 Hydrogen peroxide disinfection equipment

[0045] Using the present invention Figure 2 The vaporized hydrogen peroxide sterilization device shown is shown.

[0046] 1.1.2 Disinfectant

[0047] 35% hydrogen peroxide was used, provided by Shanghai Fantong Biotechnology Co., Ltd.; formaldehyde solution was purchased from Sinopharm Chemical Reagent Co., Ltd., with a concentration of 37% to 40%, batch number: 20201203.

[0048] 1.1.2 Test index bacteria

[0049] Bacillus subtilis (ATCC9372) bacterial tablets were purchased from Beijing Anjie Technology Co., Ltd., with each tablet containing 5 × 10⁻⁶ bacteria. 5 ~5×10 6 CFU / film.

[0050] 1.1.3 Hydrogen peroxide concentration probe

[0051] Purchased from Beijing Anjie Technology Co., Ltd.

[0052] 1.1.4 Hatching box and incubation eggs

[0053] Provided by a professional hatching cooperative in Shanghai, this experiment used pheasant eggs with a hatching capacity of 30,000, which were hatched in two hatching boxes, with 15,000 eggs in each box.

[0054] 1.1.5 Culture medium

[0055] Nutrient agar (batch number: 191017) and MacConkey agar (batch number: 200629): purchased from Beijing Luqiao Technology Co., Ltd.; Sabouraud dextrose agar (containing chloramphenicol, batch number: 20200319): purchased from Qingdao High-tech Industrial Park Haibo Biotechnology Co., Ltd.

[0056] 1.2 Sterilization test

[0057] 1.2.1 Disinfection methods

[0058] After the chicks peck at their shells, a hatching box is fumigated with formaldehyde of the standard dosage and concentration until hatching is complete and the remaining formaldehyde is removed.

[0059] Will Figure 2 The vaporized hydrogen peroxide sterilization device shown is combined with another hatching box to form a complete system. Figure 1 The hatching box and vaporized hydrogen peroxide disinfection device shown are integrated. Disinfection is performed using hydrogen peroxide: After the chicks peck at their shells, the 35% hydrogen peroxide solution in the disinfectant container 203 of the vaporized hydrogen peroxide disinfection device 2 is pumped into the flash evaporator 207 via the injection pump 206. The flash evaporator 207 heats the solution at 145°C to vaporize it, forming vaporized hydrogen peroxide. The vaporized hydrogen peroxide is then sent from the outlet 201 of the vaporized hydrogen peroxide disinfection device into the hatching box 1 by the blower 208. It is then evenly distributed within the hatching box 1 by the blower 3 inside the hatching box 1 until the hydrogen peroxide concentration probe 6 shows a concentration of 10 ppm. This concentration is then maintained for 20 hours.

[0060] 1.2.2 Placement of indicator bacterial sheets

[0061] Inside the hatching box 1, five indicator bacterial tablets were affixed to five different locations. Four of them were affixed to the corners inside the hatching box 1, and one was affixed to the egg tray 5 located in the middle of the hatching box 1.

[0062] 1.2.3 Sampling Method

[0063] Three sterilized cardboard boxes were placed in different parts of the hatching box 1 to collect down. After sterilization, the hatching box was opened, the three cardboard boxes were taken out, and the collected down was put into the envelopes separately using sterile envelopes and tweezers. The envelopes were sealed with tape and numbered A, B, and C. Each sample was no less than 2 grams. After sampling, the samples were sent to the laboratory for testing in a timely manner.

[0064] 1.2.4 Detection Items for Fiber Microorganisms

[0065] Total bacterial count, total Enterobacterial count, and total fungal spore count were determined using nutrient agar, MacConkey agar, and Sabouraud dextrose agar, respectively, for culturing and colony counting.

[0066] 1.2.5 Detection method for microorganisms in villi

[0067] According to the requirements of the "Disinfection Technical Specifications" (2002 edition), the nutrient agar medium was autoclaved at 121°C for 15 minutes and then cooled to 50°C before use.

[0068] ① Sample weighing: Weigh three portions of fluff, A, B and C, using sterile filter paper, each weighing 0.25 grams. Place the fluff in 100 ml of sterile distilled water, shake thoroughly, and then let stand for 30 minutes.

[0069] ② Culture medium preparation: Weigh out 20g, 22.55g and 32.55g of nutrient agar, MacConkey agar and Sabouraud dextrose agar respectively, dissolve them in 500ml of distilled water, heat in microwave oven until completely dissolved, sterilize in pressure cooker and cool to 50℃ and keep warm.

[0070] ③ Sample dilution: Dilute the settled fluff solution with sterile distilled water at a ratio of 1:10.

[0071] ④ Aseptic plate grouping: Prepare 72 aseptic plates. For each sample, two parallel samples are prepared for each item. For samples A, B, and C, prepare 24 plates for each sample. Aseptically, take 0.1 ml (to determine the total bacterial count), 1 ml (to determine the coliform count), and 1 ml (to determine the total fungal count) and put them into the aseptic plates respectively.

[0072] ⑤ Culture medium pouring: Cool the nutrient agar, MacConkey agar, and Sabouraud dextrose agar to about 45°C, and pour them into the pre-loaded petri dishes, about 15 ml per dish, while pouring and shaking to mix. After solidification, place the nutrient agar and MacConkey agar dishes in a 37°C incubator and the Sabouraud dextrose agar dishes in a 28°C incubator and incubate for 72 hours to count the colonies.

[0073] 1.2.6 Evaluation of disinfection effectiveness

[0074] Referring to "Key Technologies for Disinfection in Chicken Farms" (edited by Wu Rongfu), the bacterial elimination rate is calculated as follows: (Total number of colonies before disinfection - Total number of colonies after disinfection) / Total number of colonies before disinfection × 100%. The disinfection effect is determined based on the calculation results. A reduction of more than 80% in bacteria is considered good, a reduction of 70-80% is considered relatively good, a reduction of 60-70% is considered average, and a reduction of less than 60% is considered unqualified.

[0075] Bacterial mortality rate = (average number of colonies before disinfection - average number of colonies after disinfection) / average number of colonies before disinfection × 100%, and the logarithmic kill value = the logarithmic value of the average viable bacterial concentration in the control group - the logarithmic value of the viable bacterial concentration in the experimental group.

[0076] result

[0077] 2.1 Safety inspection of newly hatched pheasant chicks after disinfection using the method described in this invention.

[0078] After the hatching box is disinfected for 20 hours using the method described in this invention, the hatched pheasant chicks are as follows: Figure 3 As shown, by Figure 3 It is evident that the newly hatched pheasant chicks are in normal condition, with only a slight decrease in feather color, indicating that the method for disinfecting the hatching box based on vaporized hydrogen peroxide provided in this invention has no adverse effects on the newly hatched chicks and their growth.

[0079] 2.2 Evaluation of the disinfection effect of spore indicator

[0080] Five spore indicators were removed from the hatching box and placed in a 37°C incubator for incubation. Two controls were also set up. After 24 hours, the indicators were removed and observed. All five spore indicators were negative, while the two controls were positive. This proves that the hatching box was disinfected by the method described in this invention, which killed the spores in the five indicators from different locations.

[0081] 2.3 After disinfection, the hygiene indicators of the hatching box were tested and the disinfection effect was evaluated.

[0082] After the hatching box was disinfected by formaldehyde fumigation and by the method described in this invention, the total number of bacteria, total number of Enterobacteriaceae, and total number of fungi in the downy fibers are shown in Table 1 and Table 2, respectively:

[0083] Table 1. Hygiene indicators of hatching boxes after formaldehyde fumigation disinfection.

[0084]

[0085] Table 2. Hygiene indicators of hatching boxes after disinfection using the method described in this invention.

[0086]

[0087] Disinfection effect evaluation:

[0088] The total number of bacterial colonies in the hatching box after formaldehyde fumigation was taken as the total number of bacterial colonies before disinfection, and the total number of bacterial colonies in the hatching box after disinfection by the method described in this invention was taken as the total number of bacterial colonies after disinfection. The elimination rate of vaporized hydrogen peroxide on bacteria and fungi in the hatching box was calculated, and the results are shown in Table 3.

[0089] Table 3. Bacterial elimination rate of hatching boxes after disinfection using the method described in this invention.

[0090]

[0091] The results in Table 3 demonstrate that the elimination rates of total bacteria, total enterobacteria, and total fungi in the hatching box after disinfection using the method described in this invention all reached over 80%, meeting the criteria for good disinfection effect.

[0092] As can be seen from Tables 1 to 3, the method described in this invention, compared with the traditional formaldehyde fumigation disinfection method, can not only more effectively reduce the bacterial and fungal index in the environment inside the hatching box, but also more effectively prevent the spread of diseases through hatched chicks, thus having a better disinfection effect.

[0093] In summary, the method for disinfecting hatching boxes based on vaporized hydrogen peroxide provided by this invention effectively reduces the bacterial and fungal indices within the hatching box by using vaporized hydrogen peroxide to disinfect the internal environment during the hatching process. This effectively prevents the spread of diseases through hatched chicks and demonstrates excellent disinfection effects. Furthermore, the decomposition products of vaporized hydrogen peroxide are water and oxygen, which are non-toxic and leave no residue. Compared to traditional formaldehyde fumigation disinfection, this method is environmentally friendly, safe, pollution-free, harmless to humans, and has no adverse effects on chicks or their growth. Therefore, this invention has significant advancements and application value compared to existing technologies.

[0094] Finally, it should be pointed out that the above description is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any changes or substitutions that can be easily conceived by those skilled in the art within the scope of the technology disclosed in the present invention should be included within the scope of protection of the present invention.

Claims

1. A method for disinfecting a hatching box based on vaporized hydrogen peroxide, characterized in that, Includes the following steps: a) The hatching box is connected to the air outlet of the vaporized hydrogen peroxide disinfection device. The hatching box has an air outlet and an air inlet. Inside the hatching box are a temperature probe, an electric heating device, a humidity probe, a water tank, a hydrogen peroxide concentration probe, a fan, and an egg cart. The egg cart contains several egg trays. The vaporized hydrogen peroxide disinfection device, temperature probe, electric heating device, humidity probe, hydrogen peroxide concentration probe, and fan are all connected to the control unit. The vaporized hydrogen peroxide disinfection device includes a shell and a disinfectant container containing hydrogen peroxide solution. The shell has a vaporization chamber that is connected to both the air inlet and outlet of the vaporized hydrogen peroxide disinfection device. An injection pump is installed outside the vaporization chamber. The vaporization chamber has a flash evaporation device and a blower located above the flash evaporation device. An air heating device is installed inside the vaporization chamber and located below the flash evaporation device. b) Transfer the hatching eggs to egg trays for hatching. When the chicks start pecking at their shells or when the hatching rate reaches 5%, turn on the vaporized hydrogen peroxide sterilization device. The hydrogen peroxide solution in the sterilizer container is pumped into the flash evaporation device, where it evaporates instantly to form vaporized hydrogen peroxide. The vaporized hydrogen peroxide is then blown out of the outlet of the vaporized hydrogen peroxide sterilization device into the hatching box by a blower. At the same time, the air inside the hatching box enters the vaporization chamber through the inlet of the vaporized hydrogen peroxide sterilization device. The air, heated by the air heating device, and the vaporized hydrogen peroxide are then blown out of the outlet of the vaporized hydrogen peroxide sterilization device into the hatching box by the blower. This cycle is repeated until the concentration of hydrogen peroxide in the hatching box reaches 4-10 ppm and is maintained at this concentration for 1-20 hours.

2. The method for disinfecting the hatching box based on vaporized hydrogen peroxide according to claim 1, characterized in that: The housing is mounted on the lower part of the inner side wall of the hatching box in a wall-mounted manner. The disinfectant container is fixed to the outside of the hatching box. The air outlet of the vaporized hydrogen peroxide disinfection device is located at the top of the housing, and the air inlet of the vaporized hydrogen peroxide disinfection device is located at the bottom of the housing. The flash evaporation device is connected to the disinfectant container through an injection pump. The blower, flash evaporation device, and injection pump are all signal-connected to the control unit.

3. The method for disinfecting the hatching box based on vaporized hydrogen peroxide according to claim 1, characterized in that: The concentration of hydrogen peroxide solution in the disinfectant container is 30%–40%; the electric heating temperature of the flash evaporation device is 110–160 °C.

4. The method for disinfecting the hatching box based on vaporized hydrogen peroxide according to claim 1, characterized in that: An electrical device is provided inside the housing and outside the vaporization chamber. The blower, flash evaporator and injection pump are all connected to the electrical device. The electrical device is signal-connected to the control unit. The control unit controls the electrical device to make the injection pump inject hydrogen peroxide solution at a flow rate of 3~20 grams / hour per cubic meter of air.

5. The method for disinfecting the hatching box based on vaporized hydrogen peroxide according to claim 1, characterized in that: In step b), the temperature for hatching eggs in the egg tray is controlled at 36.7℃~37.5℃ and the humidity is controlled at 65%~75%.

6. The method for disinfecting the hatching box based on vaporized hydrogen peroxide according to claim 1, characterized in that: In step b), during the hatching process, the temperature and humidity inside the hatching box are monitored in real time by temperature and humidity probes. The control unit is equipped with a reference temperature and a reference humidity. When the detected temperature is lower than the reference temperature, the electric heating device is activated to heat the emitted heat to increase the temperature inside the hatching box. When the detected humidity is lower than the reference humidity, water is injected into the water tank in time to increase the humidity inside the hatching box by evaporation of water in the water tank.

7. The method for disinfecting the hatching box based on vaporized hydrogen peroxide according to claim 1, characterized in that: The hatching box has a vertically fixed pile inside, and the fan is located on top of the fixed pile, with the whole unit located in the center of the hatching box.

8. The method for disinfecting the hatching box based on vaporized hydrogen peroxide according to claim 1, characterized in that: The electric heating device is located near the fan.

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