Electrolytic silver ion solution capable of completely inactivating HIV and preparation method thereof

Abstract

The present application belongs to the technical field of pharmaceutical preparation, and relates to an electrolytic silver ion solution capable of completely inactivating HIV and a preparation method thereof. The electrolytic silver ion solution uses ultrapure water as a solvent, and contains divalent silver ions, a complexing agent and a complexing agent in the electrolytic silver ion solution. The complexing agent is hexamethylenetetramine capable of forming a stable complex with silver ions and capable of quantitatively analyzing the concentration of silver ions. The hexamethylenetetramine is of an analytical purity grade. The complexing agent is ethylenediamine capable of forming a more stable silver-ammonia complex ion, i.e., diammine silver, and capable of doubling the concentration of the generated silver-ammonia complex ion. The concentration of the divalent silver ions is 200-300 mg.kg ‑1 -1; the highest concentration of the complexing agent is 0.9 g.kg ‑1 -1; and the highest concentration of the complexing agent is 0.55 g.kg ‑1 The complexing agent and the complexing agent of the present application have a small dosage, a high relative ratio of silver ions and a stronger activity, and the inactivation rate of HIV is as high as 99.993%.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of pharmaceutical preparations, and particularly relates to an electrolytic silver ion solution capable of completely inactivating HIV and a preparation method thereof. BACKGROUND

[0002] Acquired Immune Deficiency Syndrome (AIDS) is a highly fatal infectious disease. The cause of AIDS is the infection of Human Immunodeficiency Virus (HIV). HIV mainly attacks T cells in the body of a patient, destroys the cellular immune function, and causes various serious opportunistic infections.

[0003] Since the 1990s, high-efficiency anti-retroviral therapy has been used in clinical treatment of AIDS, which is also known as "cocktail therapy" or HAART. HAART can effectively inhibit the reproduction of HIV and reduce the mortality of patients.

[0004] However, the use of cocktail therapy for treating AIDS still cannot completely eliminate HIV, because HIV still survives in cells and has not been killed. Once the drug is stopped, the virus will reproduce again in large quantities, leading to the recurrence of AIDS. Therefore, at the present stage, AIDS is only a chronic disease that can be treated effectively but cannot be cured.

[0005] HIV is an RNA virus with a high replication rate and mutation rate, so patients need to take medicine for their entire life. Not only is the cost of medicine high, but also the side effects are huge.

[0006] This means that the current drugs for treating AIDS are basically in the category of viral preparations, and cannot kill HIV hidden in immune cells. Therefore, the current anti-AIDS drugs are "palliative, not curative". This is also the biggest defect of the current AIDS treatment drugs.

[0007] Innovative anti-AIDS drugs is still one of the main areas in which China lags behind countries such as the United States and Europe.

[0008] As early as 2011 and 2013, Lara H H and Mori Y reported that nano-silver can inhibit HIV, hepatitis B virus HBV, influenza virus H1N1 and other viruses. The antiviral effect of nano-silver is mainly caused by silver ions released from the 111 crystal face and 100 crystal face of nano-silver.

[0009] However, since the nano-silver is a silver particle with a particle size of about 100 nm, the nano-silver is immediately phagocytosed by phagocytes as a foreign substance after entering the body, and at the same time, the phagocytes are killed by the nano-silver, causing the immune system of the body to collapse rapidly, and thus the main organs of the body such as the lungs, liver and kidneys are rapidly infected, fail and necrose, which is the main reason why the nano-silver product cannot be used for oral administration and intravascular injection to treat AIDS.

[0010] The electrolytic silver ion solution of the present application is a metal-based drug preparation with silver ions as the center, and the valence of the silver ions is high, i.e. 2 valence, also known as high-valence electrolytic silver ion solution.

[0011] The silver ion products currently supplied on the market are mostly silver nitrate systems, and the valence of the silver ions in this system is one valence. The low-valence silver nitrate solution not only has very poor stability, but also easily reacts with chloride ions in physiological saline in the blood to form silver chloride white precipitate to embolize capillaries at all levels and cause dangerous complications. Therefore, the low-valence silver ion products currently sold on the market cannot enter the body.

[0012] Therefore, it is urgent to research and develop a drug that can safely enter the body to completely kill the HIV hidden in immune cells!

[0013] Invention patent ZL202010395234.6 discloses an antibacterial and antiviral high-valence electrolytic silver ion solution and a preparation method thereof. The patent does not report the specific killing effect of the electrolytic silver ion solution on HIV, and the amount of the complexing agent and the complexing agent used to prepare the high-valence electrolytic silver ion solution is too high, which increases the ratio of impurities and reduces the ratio of silver ions, seriously affecting the therapeutic effect. At the same time, a large number of ultrasonic oscillators and labyrinth structures are used in the preparation method, which not only has a complex structure but also consumes a lot of energy and materials. The noble metal silver particles shaken off from the cathode are wasted seriously. SUMMARY

[0014] The electrolytic silver ion solution of the present application has a low amount of complexing agent and complexing agent, a high relative ratio of silver ions, and a stronger activity, and the inactivation rate of HIV is as high as 99.993%. The preparation device of the electrolytic silver ion solution of the present application has a novel and simple structure, does not have a complex ultrasonic oscillator and a labyrinth facility, has a high degree of automation, consumes little electricity, has a high utilization rate of noble metal silver plate material, and has little waste.

[0015] In order to achieve the above-mentioned purpose, the present application adopts the following technical solutions:

[0016] An electrolytic silver ion solution capable of completely inactivating HIV, the electrolytic silver ion solution uses ultrapure water as a solvent, the electrolytic silver ion solution contains divalent silver ions, a complexing agent and a complexing agent; the complexing agent is hexamethylene tetramine which can form a stable complex with silver ions and can quantitatively analyze the concentration of silver ions; the hexamethylene tetramine is of analytical purity; the complexing agent is analytical purity ethylenediamine which can form more stable silver ammine complex ions, namely diammine silver, and can multiply the concentration of the generated silver ammine complex ions; the concentration of the divalent silver ions is 200-300 mg / kg -1 ; the maximum concentration of the complexing agent is 0.9 g / kg -1 ; the maximum concentration of the complexing agent is 0.55 g / kg -1 ; the p H value of the electrolytic silver ion solution is 10.2-10.5.

[0017] The ultrapure water is water with a resistivity of 10-12 MΩ*cm (25℃).

[0018] A preparation method of an electrolytic silver ion solution capable of completely inactivating HIV, the specific steps are as follows:

[0019] 1) Inject ultrapure water into the electrolytic cell, the horizontal distance from the upper end face of the electrolytic cell is 10 cm; the electrolytic cell is an insulator, two silver electrode plates made of high-purity silver plates are placed at both ends of the electrolytic cell, the two silver electrode plates are of the same size, a stirrer is installed at the center of the electrolytic cell, and the silver electrode plates at both ends of the electrolytic cell are connected to an automatic positive and negative electrode reversing DC power supply, without distinguishing between positive and negative electrodes, which can be positive-negative or negative-positive, and the reversal time is controlled between 1-15 minutes;

[0020] (2) Turn on the stirrer and slowly increase the speed to 800-1500 rpm, the highest speed when the water surface is stable and does not overflow is used, and the water temperature is always maintained at 15-20 0 C during the subsequent reaction process;

[0021] (3) Add the complexing agent and the complexing agent to the electrolytic cell and stir to fully dissolve the complexing agent and the complexing agent;

[0022] (4) After the complexing agent and the complexing agent are fully dissolved, the automatic positive and negative electrode reversing direct current power supply is turned on, the positive or negative electrode of the two-terminal silver electrode plate is automatically converted within a set time, the silver atoms on the high-purity silver plate are electrolyzed to obtain monovalent silver ions, and the monovalent silver ions obtained by electrolysis enter the water under the attraction of the electric field and react with the complexing agent and the complexing agent to generate a diammine silver complex with a positive divalent silver ion as the core. The two electrode plates are positive and negative at times, and negative and positive at times. The automatic positive and negative electrode reversing time is 1-15 minutes, and the periodical change is carried out, so that in addition to the silver ions generated in the water, the loss of the noble metal silver material is avoided; during the reaction process, the concentration of the positive divalent silver ions in the aqueous solution is detected every 1h, when the concentration of the silver ions is 200-300mg·kg -1 , the automatic positive and negative electrode reversing direct current power supply is turned off in time according to the need, only the stirrer is kept to rotate at a speed of 200r / min or below for aging treatment, and after the aging treatment time of 48h, the PH value of the electrolytic silver ion solution is adjusted to 10.2-10.5, so that an electrolytic silver ion solution capable of completely inactivating HIV is obtained.

[0023] The specification of the automatic positive and negative electrode reversing direct current power supply is: voltage 10-100V, current 0.5-30A, and automatic positive and negative electrode reversing time 1-15 minutes.

[0024] The preparation material of the electrolytic cell is engineering plastic, and the shape of the electrolytic cell is a cube.

[0025] The purity of the high-purity silver plate in the electrode plate is 99.9-99.99%, the thickness is 0.5-4.0mm, and the area of the electrode plate is 60-80% of the inner surface area of the two ends of the electrolytic cell.

[0026] The motor power of the stirrer is 1-6kW, the speed is variable frequency, the speed range is 60-1800r / min, and the stirrer blade is engineering plastic.

[0027] The electrolytic silver ion solution and the preparation method thereof can completely inactivate HIV, and have the following outstanding advantages and effects:

[0028] In the electrolytic silver ion solution, the highest concentration of the complexing agent and the complexing agent without therapeutic effect is only 50% or less of that of patent ZL202010395234.6, the concentration of the silver ion with therapeutic effect is doubled, thereby the side effects of the auxiliary materials are obviously reduced, the therapeutic effect of the main drug silver ion is increased, and the inactivation rate of the electrolytic silver ion solution of the present application for inactivating HIV within 1h reaches 99.993% according to the detection result of Shanghai Micro Spectrum, which is very encouraging!

[0029] (2) The complexing agent hexamethylenetetramine used in the electrolytic silver ion solution of the present application not only can form a complex with silver ions, but also can form a complex with copper ions, thereby the copper ions in the water are also inactivated, and the inactivation rate of the electrolytic silver ion solution of the present application for inactivating HIV within 1h reaches 99.993% according to the detection result of Shanghai Micro Spectrum, which is very encouraging!

[0030] Hexamethylene tetramine is a common complexing titration reagent, which realizes quantitative analysis of metal ions by forming complex with metal ions in the measured substance.

[0031] The complexing agent ethylenediamine used in the electrolytic silver ion solution of the present application is not only a common complexing agent, but also has stronger alkalinity than ammonia water. The silver-ammonia complex ion formed by ethylenediamine and silver ions is not only more stable than the silver-ammonia complex ion formed by ammonia water and silver ions, but also has a concentration 1 time higher. This is fully proved by repeated detection, whether light resistance experiment or high temperature resistance experiment or silver ion depth determination, which is consistent with the report of Chemical Teaching magazine in 1995. The stability and high concentration of the electrolytic silver ion solution are very important, which not only significantly affect the length of the effective period of the electrolytic silver ion solution, but also are crucial to the use effect and the improvement of the curative effect.

[0032] The preparation device of the electrolytic silver ion solution of the present application has novel and simple structure, low power consumption, high automation degree, and automatic periodic reversal of the positive and negative polarity of the two silver plates, without frequent manual adjustment. In addition, the silver plate electrode has no additional consumption, and does not cause waste of precious metal silver material.

[0033] The electrolytic silver ion solution of the present application has a strong inactivation rate on HIV, which is a major discovery and cannot be matched by general antiviral drugs.

[0034] The electrolytic silver ion solution of the present application is proved to be non-corrosive, non-irritating, and especially non-resistant, which can be orally taken, intravenously injected, and atomized and inhaled. BRIEF DESCRIPTION OF DRAWINGS

[0035] Figure 1 is a structural schematic view of an electrolytic cell for preparing the electrolytic silver ion solution of the present application, in which: 1, electrolytic cell, 2, anode silver plate, 3, cathode silver plate, 4, stirrer, 5, direct current power supply for automatic reversal of positive and negative poles;

[0036] Figure 2-1 、 2-2 , 2-3, 2-4 are experimental reports on killing HIV of the electrolytic silver ion solution of the present application made by Shanghai Microspectrum;

[0037] Figure 3 is an experimental report of the electrolytic silver ion solution of the present application on no hemolysis after adding the electrolytic silver ion solution of the present application into human blood;

[0038] Figure 4The research conclusion of professor Sun Jianfei of Shandong Pharmaceutical Society in 2017 on silver ion toxicity in the Journal of Pharmaceutical Research is that there is no cytotoxicity when the silver ion concentration is lower than 12.5 ppm.

[0039] Figure 5 The toxicity detection report of the half lethal dose LD 50 of the oral electrolytic silver ion solution;

[0040] Figure 6-1 , 6-2 The toxicity detection report of the half lethal dose LD 50 of the intraperitoneal injection of electrolytic silver ion solution;

[0041] Figure 7 The pathological section detection report of the lung, liver and kidney after 10 days of intravenous injection of electrolytic silver ion solution;

[0042] Figure 8 The detection report of the killing of H1N1 virus by electrolytic silver ion solution; Embodiment

[0043] The application will be further described below in combination with examples, but the following examples are only used to further illustrate the application, and are not intended to limit the application. Simple modifications or numerical replacements of the application within the scope of the intention, spirit and claims of the application are within the protection scope of the application. Example

[0044] In the GY-500 small electrolytic cell, 500 kg of ultrapure water is added, the stirrer is started at a speed of 850 rpm, and the complexing agent hexamethylene tetramine 0.4 kg is slowly added under stirring. After 10 minutes, the complexing agent ethylenediamine 0.25 kg is slowly added, and after stirring for 10 minutes, the direct current power supply of the positive and negative electrode automatic reversal is started, the voltage is adjusted to 5-35 V, the current is adjusted to 0.5-3.6 A, the water temperature is controlled between 15-20 0 C, and the silver ion concentration is detected every hour until the silver ion concentration in the solution reaches 200 mg.kg -1 C, the direct current power supply of the positive and negative electrode automatic reversal is turned off, only the stirring is maintained, but the stirring speed is reduced to about 200 rpm, and the low-speed aging is 1-2 days, and then the electrolytic silver ion solution is completely stopped, the PH is adjusted to 10.2, and the stabilized electrolytic silver ion solution is packaged and sealed the next day, and stored in a cool place, which is the formal product of the electrolytic silver ion solution of the application. Example

[0045] In GY-1000 medium electrolytic cell, add 1000kg of ultrapure water, start the stirrer, rotate at 1100rpm, and slowly add complexing agent hexamethylene tetramine 0.9kg under stirring, 10 minutes later, slowly add complexing agent ethylenediamine 0.55kg, stir for 10 minutes, then start the positive and negative automatic reversing DC power supply, adjust the voltage to 10-40V, the current to 0.8-6.8A, and control the water temperature to be 15-20 0 C, and sample and detect the silver ion concentration every hour until the silver ion concentration in the solution reaches 250mg.kg -1 C, and sample and detect the silver ion concentration every hour until the silver ion concentration in the solution reaches 250mg.kg Example

[0046] In GY-2000 large electrolytic cell, add 2000kg of ultrapure water, start the stirrer, rotate at 1400rpm, and slowly add complexing agent hexamethylene tetramine 1.7kg under stirring, 10 minutes later, slowly add complexing agent ethylenediamine 1.0kg, stir for 15 minutes, then start the positive and negative automatic reversing DC power supply, adjust the voltage to 15-50V, the current to 1.7-14.5A, and control the water temperature to be 15-20 0 C, and sample and detect the silver ion concentration every hour until the silver ion concentration in the solution reaches 300mg.kg -1 C, and sample and detect the silver ion concentration every hour until the silver ion concentration in the solution reaches 300mg.kg

[0047] The mechanism of the electrolytic silver ion solution of the application in killing HIV is very clear and easy to understand: HIV protein is negatively charged,

[0048] Silver ion is positively charged, and the two attract and tightly combine each other, so that HIV is denatured, coagulated, degraded and killed.

[0049] The electrolytic silver ion solution of the present application was detected by Shanghai Weike Detection Technology Group Co., Ltd. on June 12, 2023, detection number: SHA01-23040893-JC-01, virus detected: HIV-1 II B strain, provided by Wuhan VWR Biopharmaceutical Co., Ltd.; C8166 virus-containing cells were provided by Wuhan VWR Biopharmaceutical Co., Ltd.; the detection result is that the inactivation rate of the electrolytic silver ion solution on HIV-1 virus is 99.993% at room temperature for 1 hour, see the attached Figure 2-1 , Figure 2-2 , Figure 2-3 , Figure 2-4 shown in the specification.

[0050] It should be noted that: the electrolytic silver ion solution is absolutely different from the general disinfectant 75% ethanol and 84 disinfectant. Although the general disinfectant can also kill HIV, it cannot be orally taken or injected into the body through blood vessels, and it cannot be sprayed into the eyes and nasal mucosa. The electrolytic silver ion solution (also known as high valence electrolytic silver ion solution) has no corrosion, no irritation, and no drug resistance, and can be orally taken, injected into the body through blood vessels, sprayed into the eyes and nasal mucosa, which has been completely proven by practice.

[0051] Since the electrolytic silver ion solution of the present application has good compatibility with physiological saline, it behaves like calcium and magnesium ions with a valence of 2 after entering the blood and cannot be phagocytosed by phagocytes, and can enter cells along with saline to kill HIV hidden in immune cells.

[0052] As shown in Figure 3 , after adding the electrolytic silver ion solution to the blood, the shape of the red blood cells is normal, and there is no change after 24 hours

[0053] without hemolysis, indicating that the electrolytic silver ion solution has good compatibility with blood.

[0054] The electrolytic silver ion solution has the characteristic of no drug resistance, and no matter how the HIV virus mutates, its virus shell is protein, and silver ions can chase and adhere to the outer protein of HIV, causing HIV to die.

[0055] The electrolytic silver ion solution of the application also has broad-spectrum antiviral performance: On February 27, 2020, Guangzhou Municipal Microorganism Institute carried out antiviral detection on the electrolytic silver ion solution, detection number: KY20200156, test strain: A / PR8 / 34H1N1, virus-carrying cells: MDCK cells, detection result: the killing rate of high-valence electrolytic silver ion solution on H1N1 virus within 30 minutes reached 75.45%; H1N1 virus is still representative in the virus series, which illustrates the broad-spectrum antiviral performance of the electrolytic silver ion solution from another angle, and the detection result of Shanghai Weipu also proves the broad-spectrum antiviral performance of the electrolytic silver ion solution, and suggests that the electrolytic silver ion solution has a higher killing rate on HIV, see attached Figure 8 .

[0056] That is to say, no matter what virus, silver ions can kill, the only difference is that the killing rate will be different for different viruses. There are 5.6 billion silver ions in 1 milliliter of 10 ppm concentration of silver ions, and the ability to kill viruses is very powerful!

[0057] In 2017, Professor Sun Jianfei of Shandong Pharmaceutical Society reported the research conclusion on the toxicity of silver ions in the Journal of Pharmaceutical Research: there is no cytotoxicity when the concentration of silver ions is less than 12.5 ppm. The report picture is attached Figure 4 .

[0058] The commonly used concentration of electrolytic silver ion solution is 2-10 ppm, which means that the electrolytic silver ion solution can not only kill HIV efficiently at the commonly used concentration, but also has no toxicity to the cells of the body!

[0059] The mechanism of electrolytic silver ion solution in killing viruses while having no toxicity to normal cells:

[0060] The inherent property of electrolytic silver ion solution is that it can bind with protein tightly. After the silver ions enter the body, they can not only bind with viruses, but also bind with normal cells, that is, the silver ions will cause damage to normal cells while killing viruses, but the average diameter of viruses is 100 nm, i.e. 0.1 um, and the diameter of cells is 15 um, which is converted into volume, the volume of cells is more than 337 times the volume of viruses! When the concentration of silver ions is 1-10 ppm, it can effectively kill viruses with small volume, but cannot kill cells with large volume, and only has a slight stimulation to cells; this is consistent with the research results of Professor Sun Jianfei: there is no cytotoxicity when the concentration of silver ions is less than 12.5 ppm.

[0061] This can also be said that the electrolytic silver ion solution has almost no side effects while killing HIV.

[0062] The results of the following practices can prove this point: oral toxicity test of electrolytic silver ion solution (see attached Figure 5 ), intraperitoneal injection toxicity test (see attached Figure 6-1 , 6-2 ), liver and kidney function test and lung liver and kidney pathological section test after 10 days of intravenous injection of animals (see attached Figure 7 ), all show that the concentration of electrolytic silver ion solution is 1-10 ppm, which has no cytotoxicity to normal cells; Figure 7 1, 2, and 3 are control groups, and 4, 5, and 6 are experimental groups.

[0063] Electrolytic silver ion solution is colorless and transparent, non-irritating, non-corrosive, and non-resistant. It looks like mineral water and can be stored at room temperature for 2-4 years.

[0064] Electrolytic silver ion solution can be mass-produced industrially, especially with simple and convenient methods: oral, intravenous drip, intramuscular injection, intraperitoneal and intrathoracic injection, and aerosol inhalation. It is affordable and easy to use in large areas, especially suitable for China's national conditions.

[0065] Silver and iron are both heavy metals in strict academic terms, but silver and iron are biophilic metals, which are trace elements in human tissues. Trace amounts of silver are harmless to the human body. According to the latest WHO "World Health Organization Drinking Water Quality Guidelines" fourth edition, drinking water with a silver ion concentration of less than 0.1 ppm will not cause adverse effects on the human body.

[0066] Silver is divided into nano silver, colloidal silver, and silver ions. Nano silver and colloidal silver are elemental silver, which may accumulate in the body, but electrolytic silver ion solution is in ionic state with positive charge. The electrostatic field strength of electrolytic silver ion with positive charge is greater than its own weight, so silver ions will not precipitate and accumulate.

[0067] Coombs C J used sulfadiazine silver on 22 burn patients for 6 hours in 1992, and the highest content of silver ions in the blood reached 310 ug.kg -1 . The amount of silver ions excreted from the urine of patients per day was 100-400 ug, and silver ions could be safely excreted outside the body after 15 days, without accumulation in the body and environmental pollution. The concentration of silver ions in the blood when using sulfadiazine silver to treat burn wounds can reach 20 mg.kg -1 .

[0068] Professor Chen Chuo of Wenzhou Medical College reported in Chinese Journal of Burns in 2004 that the silver ions in the blood of patients with Ⅱ degree burns, which entered the blood through the silver-loaded dressing, could be safely excreted into the body within 14 days through urine and returned to the pre-treatment level. The silver metabolism in the body is within the safe range. The silver content in the blood of normal people is 1.54±1.09 ug.kg -1 , and the silver content in the urine is 1.0±0.71 ug.kg -1 . After using silver dressing for 5 days, the silver content reached 3.88±4.42 ug.kg -1 and 3.68±4.99 ug.kg -1 , respectively. On the 9th day after stopping the use of silver dressing, the silver content returned to the pre-treatment level. The human body has the function of automatic metabolism and does not accumulate silver ions in the body.

[0069] The atomic weight of silver is only 107.8, which is 19.1 less than the atomic weight of iodine, which is 126.9. People have been taking iodine salt for many years without obvious toxicity.

[0070] After cisplatin, a platinum-based anti-cancer drug, was officially approved for clinical use in the United States in 1978, it immediately became a top choice for treating various malignant tumors. Silver ions, as a metal silver complex drug, are similar to metal platinum. Metal ions are more important than human life,

[0071] Silver ions, unlike general organic drugs, do not participate in biochemical reactions in the body and change their structure. They can enter the immune cells hidden with HIV in the blood and kill new HIV hidden in the cells.

[0072] Silver is one of the trace elements in human tissues. The commonly known harmful heavy metals are lead, arsenic, mercury, cadmium, and chromium, which do not contain iron and silver. Silver ions (non-silver single element) are harmless to advanced organisms such as humans, animals, and plants, and only kill low-level microbial organisms. WHO specifies that the safe value of silver for the human body is 0.05 mg.kg -1 . Trace amounts of silver are harmless to the human body. One of the important reasons for some people who are frequently infected with bacteria and viruses is the low content of silver ions in the body.

[0073] Professor Wen Haier reported at the Beijing Environmental Mutagen Society in 2014 that when rats were injected with silver ions at a concentration of 5 mg.kg -1 , the silver ion concentration in the blood of rats reached 12.5 mg.kg -1 , but the animal weight did not change, and there was no significant increase in the micronucleus rate of bone marrow red blood cells. Only the chromosomes of red blood cells were temporarily damaged. This result is consistent with the experimental detection results of Professor Sun Jianfei of Shandong Pharmaceutical Society.

[0074] In June 2021, we commissioned the School of Medicine of Henan University of Science and Technology, the Third Affiliated Hospital of Henan University of Science and Technology and the Sixth People's Hospital of Luoyang to conduct a toxicity experiment of injecting silver ion solution into the ear vein of white mice. The experimental animals were not lighter in weight, and there was no restlessness in body, normal diet and excretion before being killed on the 10th day, and there was no difference with the control group. After the animals were killed, kidney function, liver function and lung, liver and kidney tissues were observed by pathological section. The results were normal compared with the control group, see patent ZL202010395234.6. This is also consistent with the experimental test results of Professor Sun Jianfei of Shandong Pharmaceutical Society and others.

[0075] Although some scholars reported in the past decade that silver ions released from the surface of nano-silver have antiviral properties, why has it not been used in clinical applications so far? It is because it is limited by historical conditions at that time, and electrolytic silver ion solution has not yet appeared.

[0076] It needs to be emphasized that: the electrolytic silver ion solution is a high valence silver ion. The valence of silver ions is different, the preparation method is different, and the physicochemical properties of silver ions are completely different. Only the electrolytic silver ion solution that can safely enter the blood is the nemesis of HIV! The valence of silver ions can be easily identified with 1% sodium hydroxide solution.

[0077] The electrolytic silver ion solution of the present application is a high valence silver ion with a valence of +2. Its characteristics are that it is obtained by electrolysis in an electrolytic cell using a polar electric field, and it does not contain any nitrate component. This is unmatched by the commonly seen nitrate system, nano-silver system, and hydrogen peroxide silver ion system on the market.

[0078] The oral toxicity of the electrolytic silver ion solution (also a high valence electrolytic silver ion solution) of the present application was detected by a third party: the half lethal dose LD50 of mice was >5000mg, which was practically non-toxic (see attached Figure 5 ).

[0079] The injection toxicity of the electrolytic silver ion solution of the present application was detected by a third party: 1. According to the concentration of the administered drug, the half lethal dose was LD50=374.11ppm; 2. According to the body weight of the experimental animals, the half lethal dose was LD50=3.74mg / kg body weight (see attached figure 6).

[0080] The above test results show that the injection toxicity of the electrolytic silver ion solution is slight. When a mouse is injected intraperitoneally with a conventional factory depth of 50ppm, the maximum dose cannot cause the mouse to die, which is consistent with our animal experiment results and the detection results of the third party.

[0081] The silver ion in the electrolytic silver ion solution has a redox potential of 1.987V, which is much higher than the redox potential of 0.798V of the monovalent silver ion, and the redox potential of the divalent silver ion is more than 300 times that of the monovalent silver ion, so it is also called active silver ion.

[0082] Since the novel preparation method is adopted, the product cost is obviously reduced, and the product is convenient for large-area popularization and use, and is particularly suitable for the national conditions of China.

Claims

1. An electrolytic silver ion solution capable of completely inactivating HIV, characterized in that: The electrolytic silver ion solution takes ultrapure water as solvent, the electrolytic silver ion solution contains positive divalent silver ion, complexing agent and complexing agent; the complexing agent is hexamethylene tetramine which can form stable complex with silver ion and can quantitatively analyze the concentration of silver ion; the hexamethylene tetramine is analytical pure grade; the complexing agent is analytical pure grade ethylenediamine which can form more stable silver ammonia complex ion, namely diammine silver, and can multiply improve the concentration of generated silver ammonia complex ion; the concentration of positive divalent silver ion is 200-300 mg.kg -1 ; the highest concentration of the complexing agent is 0.9 g.kg -1 ; the highest concentration of the complexing agent is 0.55 g.kg -1 ; the p H value of the electrolytic silver ion solution is 10.2-10.

5.

2. The electrolytic silver ion solution capable of completely inactivating HIV as described in claim 1, characterized in that: The ultrapure water is water with a resistivity of 10-12 MΩ*cm at 25℃.

3. The method for preparing the electrolytic silver ion solution capable of completely inactivating HIV according to any one of claims 1-2, comprising the following steps: characterized in that: 1) injecting ultrapure water into an electrolytic cell, with the water level being 10 cm from the upper end face of the electrolytic cell; the electrolytic cell is an insulator, and silver electrode plates made of high-purity silver plates are placed at both ends of the electrolytic cell, the two silver electrode plates are of the same size, a stirrer is installed at the center of the electrolytic cell, and the silver electrode plates at both ends of the electrolytic cell are connected to an automatic positive and negative electrode reversing direct-current power supply, without distinction between positive and negative electrodes, which can be positive-negative electrodes or negative-positive electrodes, and the reversal time is controlled to be 1-15 minutes; (2) Start the stirrer and slowly increase the speed to 800-1500 rpm, with the highest speed that the water surface is stable without overflow, and always keep the water temperature at 15-20 0 C; (3) adding complexing agents and complexing agents into the electrolytic cell and stirring to fully dissolve the complexing agents and complexing agents; (4) After the complexing agent and the coordination agent are fully dissolved, the automatic positive and negative electrode reversal direct current power is turned on, so that the positive or negative electrode of the two-terminal silver electrode plate is automatically switched within a set time, the silver atoms on the high-purity silver plate are electrolyzed to obtain monovalent silver ions, the monovalent silver ions obtained by electrolysis enter the water under the attraction of the electric field, and then undergo a complexing reaction with the complexing agent and the coordination agent to generate a diammine silver complex with a positive divalent silver ion as the core. The two electrode plates are positive-negative and negative-positive at times, and the positive and negative electrodes are automatically reversed for 1-15 minutes, periodically changing, so that in addition to the silver ions generated in the water, the additional loss of precious metal silver material is avoided; during the reaction process, the concentration of the positive divalent silver ions in the aqueous solution is detected every 1 h, when the concentration of silver ions is 200-300 mg.kg -1 , the automatic positive and negative electrode reversal direct current power is turned off in a timely manner according to the need, only the stirrer is kept at a speed below 200 rpm for aging treatment, and after the aging treatment time of 48 h, the PH value of the electrolytic silver ion solution is adjusted to 10.2-10.5, thereby obtaining an electrolytic silver ion solution capable of completely inactivating HIV.

4. The method for preparing an electrolytic silver ion solution capable of completely inactivating HIV as described in claim 3, characterized in that: The specifications of the automatic positive and negative electrode reversing direct-current power supply are: voltage 10-100 V, current 0.5-30 A, and automatic positive and negative electrode reversal time 1-15 minutes.

5. The method for preparing an electrolytic silver ion solution capable of completely inactivating HIV as described in claim 3, characterized in that: The preparation material of the electrolytic cell is engineering plastic, and the shape of the electrolytic cell is a cube.

6. The method for preparing an electrolytic silver ion solution capable of completely inactivating HIV as described in claim 3, characterized in that: The purity of the high-purity silver plate in the electrode plate is 99.9-99.99%, the thickness is 0.5-4.0 mm, and the area of the electrode plate is 60-80% of the inner surface area of the two ends of the electrolytic cell.

7. The method for preparing an electrolytic silver ion solution capable of completely inactivating HIV as described in claim 3, characterized in that: The motor power of the stirrer is 1-6 kW, the speed is variable frequency, the speed range is 60-1800 revolutions, and the stirrer blade is engineering plastic.

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