A double-flower exosome fullerene composition, its preparation method and uses

Abstract

The present invention relates to a honeysuckle exosome fullerene composition, a preparation method thereof and uses thereof. The composition is composed of honeysuckle exosomes, fullerenes and a compound plant dried flower nanopowder-soaked oil. Among them, the honeysuckle exosomes are composed of one of honeysuckle exosomes or lonicera confusa exosomes, or honeysuckle exosomes and lonicera confusa exosomes in any proportion, and the compound plant dried flower nanopowder-soaked oil is obtained by soaking a compound plant dried flower nanopowder of guanyin flower, olive flower, lilac flower, dahurian angelica root flower and chimonanthus salicifolius in a compound seed oil composed of cactus seed oil extract, cucumber seed oil extract and papaya seed oil extract. The composition of the present invention has no irritation to mucous membranes, facial skin and other parts of the skin, and can be directly used on the above parts to exert health care effects.

Description

Technical Field

[0001] The present invention relates to the field of health care technologies, and particularly to a honeysuckle and flos lonicerae confusae exosome fullerene composition with health care effects, a preparation method thereof, and uses thereof. Background Art

[0002] Although there are many products containing aromatic plant infused oils on the market, their effects are often single and cannot meet the comprehensive health care needs of consumers. Therefore, it is necessary to develop new products to further meet the urgent needs of people for health care products. Summary of the Invention

[0003] In view of the deficiencies of the prior art, the present invention provides a honeysuckle and flos lonicerae confusae exosome fullerene composition, a preparation method thereof, and uses thereof.

[0004] The specific technical solutions adopted by the present invention are as follows:

[0005] In the first aspect, the present invention provides a honeysuckle and flos lonicerae confusae exosome fullerene composition, which, by mass percentage, is composed of 0.5-10% of honeysuckle and flos lonicerae confusae exosomes, 3-10% of fullerenes, and 80-96.5% of composite plant dried flower nanopowder infused oil;

[0006] The honeysuckle and flos lonicerae confusae exosomes are composed of one or both of honeysuckle exosomes and flos lonicerae confusae exosomes;

[0007] The fullerenes are composed of fullerene C60 and fullerene C70;

[0008] The composite plant dried flower nanopowder infused oil is obtained by infusing composite plant dried flower nanopowder in composite seed oil; the composite plant dried flower nanopowder is composed of nanopowders of guanyin flower, olive flower, lilac flower, angelica dahurica flower, and chimonanthus salicifolius; the composite seed oil is composed of prickly pear seed oil, cucumber seed oil, and papaya seed oil.

[0009] As a preference of the above first aspect, by mass percentage, the composition is composed of 4-6% of honeysuckle and flos lonicerae confusae exosomes, 4-6% of fullerenes, and 88-92% of composite plant dried flower nanopowder infused oil.

[0010] Further preferably, by mass percentage, the composition is composed of 5.5% of honeysuckle and flos lonicerae confusae exosomes, 5.5% of fullerenes, and 89% of composite plant dried flower nanopowder infused oil.

[0011] As a preference of the above first aspect, the honeysuckle and flos lonicerae confusae exosomes are composed of one of honeysuckle exosomes and flos lonicerae confusae exosomes or honeysuckle exosomes and flos lonicerae confusae exosomes in any proportion;

[0012] The fullerenes are composed of fullerene C60 and fullerene C70 with a mass ratio of 19:1;

[0013] The composite plant dried flower nanopowder-soaked oil is obtained by soaking the composite plant dried flower nanopowder in the composite seed oil, where the mass ratio of the composite plant dried flower nanopowder to the composite seed oil is 1:8.5;

[0014] The composite plant dried flower nanopowder is composed of nanopowders of Guanyin flower, olive flower, lilac flower, angelica dahurica flower, and Chimonanthus salicifolius with a mass ratio of 55:5:15:20:5;

[0015] The composite seed oil is composed of prickly pear seed oil, cucumber seed oil, and papaya seed oil with a mass ratio of 2:4.5:3.5.

[0016] As a preference of the above first aspect, the preparation method of the double-flower exosomes includes the following steps: using fresh Lonicera japonica or Lonicera confusa as raw materials respectively, after tissue disruption of the raw materials at -40°C, centrifuging at 3000 r / min for 45 min and then at 9500 r / min for 45 min in sequence, collecting the supernatant, adding the supernatant to a ZrO2:TiO2 column with a mass ratio of 1:1 for adsorption for 3.5 h, then using buffer PBS as the eluent for column elution, centrifuging the obtained eluent at 30000 r / min for 1.5 h, and freeze-drying the obtained precipitate to prepare the exosomes corresponding to the raw materials.

[0017] As a preference of the above first aspect, the preparation method of the composite plant dried flower nanopowder-soaked oil includes the following steps:

[0018] S1: Using Guanyin flower, olive flower, lilac flower, angelica dahurica flower, and Chimonanthus salicifolius as raw materials respectively, after low-temperature vacuum microwave drying, crushing and passing through a 100-mesh sieve, the sieved material is further crushed under the conditions of a crushing gas pressure of 0.95 MPa, a gas consumption of 1.6 m 3 / min, a crushing wheel speed of 15000 r / min, and a feeding speed of 0.65 kg / h. At the same time, liquid nitrogen is input into the crushing chamber during the crushing process. After air classification of the crushed material, the nanopowders corresponding to each raw material are obtained respectively;

[0019] S2: Uniformly mixing the nanopowders of Guanyin flower, olive flower, lilac flower, angelica dahurica flower, and Chimonanthus salicifolius according to a mass ratio of 55:5:15:20:5 to obtain the composite plant dried flower nanopowder, and soaking it in the composite seed oil composed of prickly pear seed oil, cucumber seed oil, and papaya seed oil with a mass ratio of 2:4.5:3.5, where the mass ratio of the composite plant dried flower nanopowder to the composite seed oil is 1:8.5, stirring 2 times a day, each time for 1 h, and the stirring speed is 190 rpm; after soaking for 3 to 30 days, the composite plant dried flower nanopowder-soaked oil is obtained.

[0020] Further preferably, the soaking time of the composite plant dried flower nanopowder in the composite seed oil is 5 to 10 days.

[0021] In a second aspect, the present invention provides a method for preparing a double-flower exosome fullerene composition as described in any one of the first aspects, which comprises the following steps: under the preparation conditions of a stirring speed of 100 to 120 rpm and a temperature of 37 to 39 °C, according to their respective material mass ratios, the fullerene is first fully stirred and mixed with the double-flower exosomes to obtain a mixture, the mixing time is 42 to 50 min, and then under the same preparation conditions, the mixture is further mixed with the soaking oil of the composite plant dried flower nanopowder for 1.2 to 3 h to obtain a double-flower exosome fullerene composition.

[0022] In a third aspect, the present invention provides a double-flower exosome fullerene combination prepared by the preparation method described in the second aspect above.

[0023] In a fourth aspect, the present invention provides the use of a double-flower exosome fullerene composition as described in any one of the first aspects or the third aspect above in the preparation of an anti-inflammatory drug against inflammatory factor IL-1β.

[0024] In a fifth aspect, the present invention provides the use of a double-flower exosome fullerene composition as described in any one of the first aspects or the third aspect above in the preparation of a drug with acne-removing efficacy.

[0025] In a sixth aspect, the present invention provides the use of a double-flower exosome fullerene composition as described in any one of the first aspects or the third aspect above in the preparation of a rhinitis-improving drug or product.

[0026] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0027] (1) The double-flower exosome fullerene composition disclosed in the present invention includes three components: fullerene, double-flower exosomes, and the soaking oil of the composite plant dried flower nanopowder, and the three have a synergistic effect.

[0028] (2) Through repeated experiments, the present invention found that when using a double-flower exosome prepared from one of honeysuckle exosomes and lonicera confusa exosomes or a combination of honeysuckle exosomes and lonicera confusa exosomes in any proportion to prepare the composition of the present invention, its health care effects are basically the same.

[0029] (3) In the present invention, the fullerene is first fully mixed and wrapped with the double-flower exosomes, and then mixed with the soaking oil of the composite plant dried flower nanopowder to prepare a double-flower exosome fullerene composition, and its anti-inflammatory effect is more ideal.

[0030] (4) Through systematic experimental research, it is found in the present invention that a composite plant dried flower nanopowder-soaked oil prepared by using a composite seed oil composed of prickly pear seed oil, cucumber seed oil and papaya seed oil as the base oil can further exert an anti-inflammatory effect when used to prepare a double-flower exosome fullerene composition.

[0031] (5) The composition of the present invention can help the skin resist inflammation, condition the skin barrier, repair damaged cell regeneration, and also has the effects of treating acne and improving rhinitis. Specific embodiments

[0032] To better understand the technical content of the present invention, specific embodiments are provided below to further illustrate the present invention.

[0033] In the present invention, a double-flower exosome fullerene composition is provided. By mass percentage, the composition comprises the following components:

[0034] Double-flower exosomes 0.5 - 10%;

[0035] Fullerene 3 - 10%;

[0036] Composite plant dried flower nanopowder-soaked oil 80 - 96.5%.

[0037] Among the components of the above composition of the present invention, the double-flower exosomes are composed of one of honeysuckle exosomes and lonicera confusa exosomes, or a mixture of honeysuckle exosomes and lonicera confusa exosomes in any proportion.

[0038] Among the components of the above composition of the present invention, the fullerene is composed of fullerene C60 and fullerene C70 with a mass ratio of 19:1.

[0039] Among the components of the above composition of the present invention, the composite plant dried flower nanopowder-soaked oil is obtained by soaking the composite plant dried flower nanopowder in the composite seed oil. The mass ratio of the composite plant dried flower nanopowder to the composite seed oil is 1:8.5. The composite plant dried flower nanopowder is composed of nanopowders of guanyin flower, olive flower, lilac flower, angelica dahurica flower and chimonanthus salicifolius with a mass ratio of 55:5:15:20:5, and the composite seed oil is composed of prickly pear seed oil, cucumber seed oil and papaya seed oil with a mass ratio of 2:4.5:3.5.

[0040] Among the components of the above composition of the present invention, the preparation method of the double-flower exosomes (denoted as preparation method A) comprises the following steps:

[0041] Using fresh honeysuckle or lonicera hypoglauca miq as raw materials respectively, after tissue disruption of the raw materials at -40°C, centrifuging at 3000 r / min for 45 min and then at 9500 r / min for 45 min in sequence, collecting the supernatant, adding the supernatant to a ZrO2:TiO2 column with a mass ratio of 1:1 for adsorption for 3.5 h, then using buffer PBS as the eluent for column elution, centrifuging the obtained eluent at 30000 r / min for 1.5 h, and freeze-drying the obtained precipitate to prepare the exosomes corresponding to the raw materials.

[0042] Among the components of the above composition of the present invention, the preparation method of the composite plant dried flower nanopowder immersion oil (denoted as preparation method B) includes the following steps:

[0043] S1: Respectively using Guanyin flower, olive flower, lilac flower, angelica dahurica flower and Chimonanthus salicifolius as raw materials, after low-temperature vacuum microwave drying the raw materials, crushing and sieving through a 100-mesh sieve, and then crushing the sieved materials under the conditions of a crushing gas pressure of 0.95 MPa, a gas consumption of 1.6 m 3 / min, a crushing wheel speed of 15000 r / min, and a feeding speed of 0.65 kg / h, while inputting liquid nitrogen into the crushing chamber during the crushing process, and after air separation and classification of the crushed materials, obtaining the nanopowders corresponding to each raw material respectively;

[0044] S2: Uniformly mixing the nanopowders of Guanyin flower, olive flower, lilac flower, angelica dahurica flower and Chimonanthus salicifolius according to a mass ratio of 55:5:15:20:5 to obtain a composite plant dried flower nanopowder, and soaking it into a composite seed oil composed of prickly pear seed oil, cucumber seed oil and papaya seed oil with a mass ratio of 2:4.5:3.5, wherein the mass ratio of the composite plant dried flower nanopowder to the composite seed oil is 1:8.5, stirring 2 times a day, each time for 1 h, with a stirring speed of 190 rpm; after soaking for 3 to 30 days, obtaining the composite plant dried flower nanopowder immersion oil. Further, the soaking time is preferably 5 to 10 days.

[0045] In a further preferred embodiment provided by the present invention, by mass percentage, the components of the composition are as follows:

[0046] Double-flower exosomes 4 - 6%;

[0047] Fullerene 4 - 6%;

[0048] Composite plant dried flower nanopowder immersion oil 88 - 92%.

[0049] In a preferred embodiment of the present invention, an optimal component formulation of the above double-flower exosome fullerene composition is provided. By mass percentage, the components of the composition are as follows:

[0050] Double-flower exosomes 5.5%;

[0051] Fullerene 5.5%;

[0052] Immersion oil of composite plant dried flower nanopowder 89%.

[0053] The present invention also provides a preparation method of the double-flower exosome fullerene composition, comprising the following steps: under the conditions of a stirring speed of 110 rpm and a temperature of 38°C, fullerene is first fully stirred and mixed with double-flower exosomes, and the mixing time is 42 - 50 min. Then, under the same conditions, the above mixture is mixed with the immersion oil of composite plant dried flower nanopowder, and the mixing time is 1.25 h.

[0054] Next, the present invention uses specific examples to demonstrate the efficacy of the above double-flower exosome fullerene composition and the influence of different component formulations on the final anti-inflammatory and health care effects such as improving rhinitis. The preparation methods of the double-flower exosomes and the immersion oil of composite plant dried flower nanopowder used in each example are respectively as described in the aforementioned Preparation Method A and Preparation Method B, and will not be elaborated here.

[0055] Examples

[0056] (1) Components of the double-flower exosome fullerene composition in Examples 1 - 5

[0057] In order to investigate whether there is a significant impact on the health care efficacy of the double-flower exosome fullerene composition when honeysuckle exosomes, lonicera hypoglauca exosomes are used as double-flower exosomes, or a combination of honeysuckle exosomes and lonicera hypoglauca exosomes in any proportion is used as double-flower exosomes, Examples 1 - 5 are designed. By mass percentage, the raw materials include the following (Table 1):

[0058] Table 1

[0059]

[0060] In addition, the component ratios within each raw material category in Examples 1 - 5 are as follows:

[0061] Fullerene is composed of fullerene C60 and fullerene C70 with a mass ratio of 19:1;

[0062] The immersion oil of composite plant dried flower nanopowder is obtained by soaking composite plant dried flower nanopowder in composite seed oil, where the mass ratio of composite plant dried flower nanopowder to composite seed oil is 1:8.5;

[0063] The composite plant dried flower nanopowder is composed of nanopowders of guanyin flower, olive flower, lilac flower, angelica dahurica flower and chimonanthus salicifolius with a mass ratio of 55:5:15:20:5;

[0064] The composite seed oil is composed of prickly pear seed oil, cucumber seed oil and papaya seed oil with a mass ratio of 2:4.5:3.5.

[0065] (2) Preparation of the bifloral exosome fullerene composition in Examples 1 - 5

[0066] Under the conditions of a stirring speed of 110 rpm and a temperature of 38 °C, fullerene is first fully stirred and mixed with bifloral exosomes for a mixing time of 42 - 50 min. Then, under the same conditions, the above mixture is mixed with the composite plant dried flower nanopowder - soaked oil for a mixing time of 1.25 h.

[0067] (3) Influence of the composition in Examples 1 - 5 on the secretion of the inflammatory factor IL - 1β by THP - 1 cells

[0068] The secretion amount of inflammatory factors is an intuitive indicator of the occurrence of acne inflammation. Since IL - 1β is specifically produced when human acute monocytic THP - 1 has an inflammatory reaction, IL - 1β is often used as an index for acne inflammation - related screening.

[0069] Take THP - 1 cells in the exponential growth phase and inoculate them into a 96 - well culture plate at a density of approximately 1×10 6 cells / mL, and co - incubate them with the bifloral exosome fullerene compositions of Examples 1 - 5 at a mass concentration of 1.25 mg / L for 4 h. Prepare a live bacteria suspension from Propionibacterium acnes anaerobically cultured for 72 h and add it to the 96 - well plate at a ratio of 100:1 of Propionibacterium acnes to THP - 1 cells. Incubation without adding drugs is used as a negative control, incubation with retinoic acid (25 μmol / L) is used as a positive control, and adding only phosphate - buffered saline (PBS) is used as a blank control. Culture for 24 h and detect the content of IL - 1β according to the instructions of the ELISA kit.

[0070] IL - 1β inhibition rate = (decrease value of secretion amount / initial value of secretion amount) × 100%

[0071] Propionibacterium acnes induces THP - 1 cells to secrete a large amount of the inflammatory factor IL - 1β, and the increase is about 85.2 times. The inhibition rate of the positive control on IL - 1β reaches 91.6%. The inhibition rates of Examples 1 - 5 at the same concentration on the inflammatory factor IL - 1β are 94.3%, 93.9%, 94.7%, 93.5% and 94.0% respectively. From the results, it is judged that Examples 1 - 5 all have an obvious effect of inhibiting the secretion of the inflammatory cytokine IL - 1β.

[0072] From the analysis of the inhibition rate results of inflammatory cytokine IL-1β in Examples 1 to 5, the adjustment of the component ratio of honeysuckle exosomes and lonicera confusa exosomes in double-flower exosomes has no obvious effect on the inhibition rate of inflammatory factor IL-1β. Whether the double-flower exosomes are composed of one of honeysuckle exosomes and lonicera confusa exosomes, or any proportion combination of honeysuckle exosomes and lonicera confusa exosomes, the inhibition rate of inflammatory factor IL-1β is basically similar. Generally speaking, the best effect is in Example 3, which is slightly higher than the other several examples.

[0073] Therefore, in the subsequent examples, the double-flower exosomes are composed of honeysuckle exosomes and lonicera confusa exosomes with a mass ratio of 1:1.

[0074] (4) Components of the double-flower exosome fullerene composition in Examples 6 to 11

[0075] In order to investigate the influence of each raw material of double-flower exosomes, fullerenes and the immersion oil of composite plant dried flower nanopowders on the anti-inflammatory activity of the double-flower exosome fullerene composition, Examples 6 to 11 were designed. By mass percentage, it includes the following raw materials (Table 2):

[0076] Table 2

[0077]

[0078] (5) Influence of the composition in Examples 6 to 11 on the secretion of inflammatory factor IL-1β by THP-1 cells

[0079] Using the same anti-inflammatory experimental method as in Examples 1 to 5, the influence of Examples 6 to 11 on the secretion of inflammatory factor IL-1β by THP-1 cells was investigated. The inhibition rates of Examples 6 to 11 on inflammatory factor IL-1β were 75.1%, 76.5%, 84.8%, 83.4%, 85.5% and 73.3% respectively. It can be seen from the results that with different raw material ratios, there are obvious differences in the inhibition results of inflammatory factor IL-1β.

[0080] Comparing the inhibition rates of Examples 1 to 11 on inflammatory factor IL-1β, it was found that the anti-inflammatory result of Example 3 was the best. When the mass ratio of double-flower exosomes to fullerenes was 1:1, the anti-inflammatory effect of the double-flower exosome fullerene composition was more ideal.

[0081] (6) Components of the double-flower exosome fullerene composition in Control Examples 1 to 6

[0082] To show whether the combination of double-flower exosomes, fullerenes and the immersion oil of composite plant dried flower nanopowders in the raw materials is a necessary component, Control Examples 1 to 6 were designed by comparing Example 3. By mass percentage, it includes the following raw materials (Table 3):

[0083] Table 3

[0084]

[0085] (7) Effects of the compositions in Comparative Examples 1 - 6 on the secretion of inflammatory factor IL-1β by THP-1 cells

[0086] Using the same anti-inflammatory experimental method as in Examples 1 - 11, the effects of Comparative Examples 1 - 6 on the secretion of inflammatory factor IL-1β by THP-1 cells were investigated. The inhibition rates of Comparative Examples 1 - 6 on inflammatory factor IL-1β were 52.6%, 56.9%, 42.4%, 39.4%, 36.9% and 44.0% respectively. From the results, it can be seen that after the combination of honeysuckle exosomes, fullerene and the soaking oil of composite plant dried flowers in a certain proportion, its anti-inflammatory effect is obvious, such as Example 3.

[0087] (8) Components and anti-inflammatory activities of the honeysuckle exosome-fullerene compositions in Comparative Examples 7 - 14

[0088] To show whether prickly pear seed oil, cucumber seed oil and papaya seed oil in the composite seed oil in the raw materials are necessary components, Comparative Examples 7 - 14 were designed by comparing with Example 3. Compared with Example 3, except for the difference in the composite seed oil, the other raw materials of Comparative Examples 7 - 14 are the same as those of Example 3. Specifically: Comparative Example 7 (the composite seed oil consists of a single prickly pear seed oil), Comparative Example 8 (the composite seed oil consists of a single cucumber seed oil), Comparative Example 9 (the composite seed oil consists of a single papaya seed oil), Comparative Example 10 (the composite seed oil consists of prickly pear seed oil and cucumber seed oil with a mass ratio of 2:4.5), Comparative Example 11 (the composite seed oil consists of prickly pear seed oil and papaya seed oil with a mass ratio of 2:3.5), Comparative Example 12 (the composite seed oil consists of cucumber seed oil and papaya seed oil with a mass ratio of 4.5:3.5), Comparative Example 13 (the composite seed oil consists of prickly pear seed oil, cucumber seed oil and papaya seed oil with a mass ratio of 2:4:3), Comparative Example 14 (the composite seed oil consists of prickly pear seed oil, cucumber seed oil and papaya seed oil with a mass ratio of 1:1:1).

[0089] Using the same anti-inflammatory experimental method as in Examples 1 - 11, the effects of Comparative Examples 7 - 14 on the secretion of inflammatory factor IL-1β by THP-1 cells were investigated. The inhibition rates of Comparative Examples 7 - 14 on inflammatory factor IL-1β were 55.5%, 59.2%, 57.9%, 62.8%, 63.7%, 59.9%, 90.6% and 89.9% respectively. From the results, it can be seen that the composite seed oil consisting of prickly pear seed oil, cucumber seed oil and papaya seed oil with a mass ratio of 2:4.5:3.5 has better anti-inflammatory advantages.

[0090] (9) Components and anti-inflammatory activities of the honeysuckle exosome-fullerene compositions in Comparative Examples 15 - 20

[0091] To further investigate whether compound seed oil can be replaced by other raw materials, referring to the design principles of Comparative Examples 7-14, in comparison with Example 3, Comparative Examples 15-20 were designed by replacing compound seed oil with other raw materials. Comparative Example 15 (compound seed oil replaced by olive oil), Comparative Example 16 (compound seed oil replaced by coconut oil), Comparative Example 17 (compound seed oil replaced by sunflower oil), Comparative Example 18 (compound seed oil replaced by wheat germ oil), Comparative Example 19 (compound seed oil replaced by soybean oil), and Comparative Example 20 (compound seed oil replaced by corn germ oil).

[0092] Using the same anti-inflammatory experimental method as in Examples 1-11, the effects of Comparative Examples 15-20 on the secretion of inflammatory factor IL-1β by THP-1 cells were investigated. The inhibition rates of Comparative Examples 15-20 on inflammatory factor IL-1β were 51.7%, 55.5%, 51.9%, 55.9%, 45.3% and 39.8% respectively. It can be seen from the results that after replacing compound seed oil with other raw materials, the anti-inflammatory activity of the honeysuckle exosome fullerene composition is significantly reduced.

[0093] (10) Components and anti-inflammatory activity of the honeysuckle exosome fullerene composition of Comparative Examples 21-22

[0094] To show the effect of the composition ratio of the composite plant dried flower nanoparticles on the anti-inflammatory activity of the honeysuckle exosome fullerene composition, in comparison with Example 3, Comparative Example 21 was designed, and its composite plant dried flower nanoparticles were composed of nanoparticles of Guanyin flower, olive flower, lilac flower, angelica dahurica flower and Chimonanthus salicifolius with a mass ratio of 1:1:1:1:1; Comparative Example 22, its composite plant dried flower nanoparticles were composed of nanoparticles of Guanyin flower, olive flower, lilac flower, angelica dahurica flower and Chimonanthus salicifolius with a mass ratio of 3:1:1:4:1.

[0095] Using the same anti-inflammatory experimental method as in Examples 1-11, the effects of Comparative Examples 21-22 on the secretion of inflammatory factor IL-1β by THP-1 cells were investigated. The inhibition rates of Comparative Examples 21-22 on inflammatory factor IL-1β were 85.1% and 87.3% respectively. It can be seen from the results that the difference in the composition ratio of each raw material in the composite plant dried flower nanoparticles has a certain effect on the anti-inflammatory activity.

[0096] (11) Components of the honeysuckle exosome fullerene composition of Comparative Example 23 and the skin feel experience of Example 3 and Comparative Example 23

[0097] In comparison with Example 3, Comparative Example 23 was designed, and the fullerene in its raw materials was composed of C60 and C70 at a ratio of 1:1; at the same time, Comparative Example 24 was also designed, and the fullerene in its raw materials was composed of C60 and C70 at a ratio of 10:1; the other raw materials and their ratios of Comparative Example 23 and Comparative Example 24 were the same as those of Example 3.

[0098] Skin-feel sensory experience experiment: The test subjects were required to be 40 healthy female volunteers aged between 25 and 35 years old. They were evenly divided into 2 groups according to age, with 20 people in each group, and the average age was 30 ± 5 years old.

[0099] More than 97% of the test subjects said that the skin feel of Example 3 was fresh, transparent, and had a fast absorption rate. The absorption rate of Comparative Example 24 was medium, while that of Comparative Example 23 was relatively slow.

[0100] (12) Application of the double-flower exosome fullerene composition in improving adult sinusitis

[0101] There were 20 subjects, aged between 40 and 65 years old, all of whom had sinusitis symptoms, lost their sense of smell for 3 - 10 years, and had tried traditional Chinese and Western medicine treatments many times without effect. The subjects used Example 3 of the present invention and applied it to the nose and forehead 2 times before going to bed every day, massaging for 5 - 10 minutes each time. Among them, 15 people's sense of smell began to recover after 60 days of use, and the remaining 5 people's sense of smell began to recover between 61 and 150 days of use.

[0102] (13) Application of the double-flower exosome fullerene composition in improving adult allergic rhinitis

[0103] There were 25 subjects, aged between 20 and 70 years old. The subjects had symptoms such as nasal itching, paroxysmal sneezing, watery nasal discharge, and unilateral or bilateral nasal congestion. Some patients also had a decrease in the sense of smell. The subjects used Example 3 of the present invention and applied it to the nose and forehead 2 times before going to bed every day, massaging for 5 - 10 minutes each time. Among them, 23 people's symptoms of nasal itching, paroxysmal sneezing, and watery nasal discharge disappeared within 30 days, and their sense of smell recovered to varying degrees. The other 2 people achieved the above effects between 50 and 60 days. All the subjects fully recovered between 90 and 120 days of use.

[0104] (14) Application of the double-flower exosome fullerene composition in improving children's allergic rhinitis

[0105] There were 5 subjects, aged between 3 and 6 years old. All the subjects had symptoms of allergic sinusitis, including dry nose, nasal itching, nasal discharge, and unilateral or bilateral nasal congestion. With the help of their parents, the subjects used Example 3 of the present invention and applied it to the nose and forehead 2 times before going to bed every day, massaging for 5 - 10 minutes each time. All the subjects fully recovered within 30 days.

[0106] (15) Application of the double-flower exosome fullerene composition in acne treatment

[0107] 15. 1) Application of double-flower exosome fullerene composition in improving acne

[0108] Thirty subjects, aged between 13 and 18, all had acne symptoms, including whiteheads, blackheads, papules and pustules on the cheeks, forehead, nose, chin and neck. The subjects used Example 3 of the present invention, applying it to the acne area or the whole face respectively every morning and evening, massaging for 5 - 10 minutes until completely absorbed. The subjects gradually recovered within 30 - 90 days. Among them, 15 subjects had more than 95% of their acne eliminated after 60 days of use; 10 subjects had more than 90% of their acne eliminated after 90 days of use; and the other 5 subjects had more than 90% of their acne eliminated after 150 days of use.

[0109] 15. 2) Application of double-flower exosome fullerene composition in improving acne

[0110] Twenty subjects, aged between 28 and 30, all had acne symptoms, including inflammatory papules and pustules on the cheeks, forehead, nose, chin and neck, and accompanied by repeated infections. The subjects had tried many treatments without success. They used Example 3 of the present invention, applying it to the acne area or the whole face respectively every morning and evening, massaging for 5 - 10 minutes until completely absorbed. The subjects gradually recovered within 60 - 120 days. Among them, 18 subjects were basically cured after 60 days of use; and the other 2 subjects had more than 95% improvement in acne after 120 days of use.

[0111] The above - described embodiments are only a preferred solution of the present invention, but they are not intended to limit the present invention. Those of ordinary skill in the relevant technical field can make various changes and modifications without departing from the spirit and scope of the present invention. Therefore, all technical solutions obtained by means of equivalent replacement or equivalent transformation fall within the protection scope of the present invention.

Claims

1. A double-flower exosome fullerene composition, characterized in that: By mass percentage, the composition consists of 0.5 - 10% of double-flower exosomes, 3 - 10% of fullerene, and 80 - 96.5% of the soaking oil of composite plant dried flower nanopowder; The double-flower exosomes are composed of one of honeysuckle exosomes and lonicera confusa exosomes, or a mixture of honeysuckle exosomes and lonicera confusa exosomes in any proportion; The fullerene is composed of fullerene C60 and fullerene C70 with a mass ratio of 19:1; The soaking oil of composite plant dried flower nanopowder is obtained by soaking the composite plant dried flower nanopowder in composite seed oil, where the mass ratio of the composite plant dried flower nanopowder to the composite seed oil is 1:8.5; The composite plant dried flower nanopowder is composed of nanopowders of guanyin flower, olive flower, lilac flower, angelica dahurica flower, and chimonanthus salicifolius with a mass ratio of 55:5:15:20:5; The composite seed oil is composed of prickly pear seed oil, cucumber seed oil, and papaya seed oil with a mass ratio of 2:4.5:3.5; 2. The double-flower exosome fullerene composition according to claim 1, wherein: By mass percentage, the composition consists of 4 - 6% of double-flower exosomes, 4 - 6% of fullerene, and 88 - 92% of the soaking oil of composite plant dried flower nanopowder; 3. The double-flower exosome fullerene composition according to claim 1, wherein: By mass percentage, the composition consists of 5.5% of double-flower exosomes, 5.5% of fullerene, and 89% of the soaking oil of composite plant dried flower nanopowder; 4. The double-flower exosome fullerene composition according to claim 1, characterized in that, The preparation method of the double-flower exosomes includes the following steps: Using fresh honeysuckle or lonicera confusa as raw materials, after tissue disruption at -40°C, centrifuge at 3000 r / min for 45 min and then at 9500 r / min for 45 min in sequence, collect the supernatant, add the supernatant to a ZrO2:TiO2 column with a mass ratio of 1:1 for adsorption for 3.5 h, then use buffer PBS as the eluent for column elution, centrifuge the obtained eluate at 30000 r / min for 1.5 h, and the resulting precipitate is freeze-dried to prepare the exosomes corresponding to the raw materials.

5. The double-flower exosome fullerene composition according to claim 1, wherein The preparation method of the soaking oil of composite plant dried flower nanopowder includes the following steps: S1: Respectively use Guanyin flower, olive flower, lilac flower, angelica dahurica flower and Chimonanthus salicifolius as raw materials. After the raw materials are dried by low-temperature vacuum microwave and then crushed and sieved through a 100-mesh sieve, the sieved materials are further crushed under the conditions of a crushing gas pressure of 0.95 MPa, a gas consumption of 1.6 m 3 / min, a crushing wheel speed of 15000 r / min, and a feeding speed of 0.65 kg / h. At the same time, liquid nitrogen is input into the crushing chamber during the crushing process. After the crushed materials are separated by air classification, the nano-powders corresponding to each raw material are obtained respectively; S2: Uniformly mix the nanopowders of guanyin flower, olive flower, lilac flower, angelica dahurica flower, and chimonanthus salicifolius according to a mass ratio of 55:5:15:20:5 to obtain the composite plant dried flower nanopowder, and soak it in the composite seed oil composed of prickly pear seed oil, cucumber seed oil, and papaya seed oil with a mass ratio of 2:4.5:3.5, where the mass ratio of the composite plant dried flower nanopowder to the composite seed oil is 1:8.5, stir 2 times a day, each time for 1 h, and the stirring speed is 190 rpm; after soaking for 3 - 30 days, obtain the soaking oil of composite plant dried flower nanopowder.

6. The double-flower exosome fullerene composition according to claim 5, characterized in that, The soaking time of the composite plant dried flower nanopowder in the composite seed oil is 5 - 10 days.

7. A preparation method of the double-flower exosome fullerene composition according to any one of claims 1 to 6, characterized in that, Including the following steps: Under the preparation conditions of a stirring speed of 100 - 120 rpm and a temperature of 37 - 39°C, according to their respective material mass ratios, first fully stir and mix the fullerene with the double-flower exosomes to obtain a mixture, and the mixing time is 42 - 50 min. Then, under the same preparation conditions, mix the mixture with the soaking oil of composite plant dried flower nanopowder, and the mixing time is 1.2 - 3 h to obtain the double-flower exosome fullerene composition.

8. A bifloral exosome fullerene composition prepared by the preparation method as described in claim 7.

9. Use of the bifloral exosome fullerene composition as described in any one of claims 1 to 6 or the bifloral exosome fullerene composition prepared by the preparation method as described in claim 7 in the preparation of an anti-inflammatory drug against inflammatory factor IL-1β.

10. Use of the bifloral exosome fullerene composition as described in any one of claims 1 to 6 or the bifloral exosome fullerene composition prepared by the preparation method as described in claim 7 in the preparation of a drug with acne-removing efficacy.

11. Use of the bifloral exosome fullerene composition as described in any one of claims 1 to 6 or the bifloral exosome fullerene composition prepared by the preparation method as described in claim 7 in the preparation of a drug for improving rhinitis.

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