Microbial sampler containing an air-filled drainage assembly and use thereof

Abstract

The present application is suitable for the technical field of environmental detection, and provides a microorganism sampler with an air-filling and water-draining assembly, which comprises a shell, a water-filtering and sampling assembly installed on the top of the shell, a water-pumping element for pumping water into the shell to make the shell sink in water and drive the water-filtering and sampling assembly to sink, a propeller assembly installed on the shell for moving the sinking shell in water, an air-filling and water-draining assembly for filling air into the shell to drain water in the shell, so that the shell floats to drive the water-filtering and sampling assembly to float and sample, and a rotating rod installed on the shell, the bottom of the rotating rod is provided with a driving rotating blade arranged in the shell, and the top of the rotating rod is fixedly provided with a cutting element.The present application has the advantages of avoiding the defects of traditional sampling, such as great difficulty in water surface sampling and easy sampling pollution, simple sampling, ensuring the accuracy of sampling research results, and reducing the complexity and labor intensity.

Description

[0001] This application is a divisional application of application number 202110934286.0, filed on August 16, 2021, with the invention title "A Microbial Sampler for Environmental Monitoring" at the time of filing. Technical Field

[0002] This invention belongs to the field of environmental monitoring technology, and in particular relates to a microbial sampler containing an air-filled drainage component and its application. Background Technology

[0003] Microorganisms are extremely sensitive to environmental pollution or changes. Utilizing microbial detection technology to detect microorganisms can effectively grasp the environmental status through microbial information, monitor and assess environmental quality from a biological perspective, and reflect the historical situation of environmental pollution. It can effectively make up for the shortcomings of physical and chemical detection and has unique advantages in environmental monitoring.

[0004] Water bodies can be divided into natural and artificial water bodies. Water contains various nutrients required by microorganisms, thus serving as their natural habitat. Common microorganisms in water include common bacteria such as green sulfur bacteria, purple bacteria, cyanobacteria, stalked bacteria, ochre-colored ciliates, spherical bacteria, and fluorescent Pseudomonas. In addition, there are many algae (such as filamentous green algae and diatoms). Sampling and testing microorganisms in the aquatic environment is an important way to assess water quality and whether water sources are polluted.

[0005] Existing microbial samplers often rely on manual sampling on the water surface when sampling microorganisms, especially floating aquatic organisms such as algae. However, manual sampling on the water surface is not only difficult (as floating objects tend to stick together), but it also easily leads to contamination from similar non-target organisms in the surrounding area, affecting the accuracy of the sampling results. Summary of the Invention

[0006] The purpose of this invention is to provide a microbial sampler containing an air-filled drainage component and its application, aiming to solve the problem that existing microbial samplers, when sampling microorganisms in water, especially floating objects such as algae on the water surface, are mostly done manually on the water surface. However, manual sampling on the water surface is not only difficult, but also easily leads to contamination by similar non-target organisms in the surrounding area, affecting the accuracy of the sampling results.

[0007] The present invention is implemented as follows: a microbial sampler containing an air-filled drainage assembly includes:

[0008] case;

[0009] The water filtration and sampling assembly is installed on the top of the housing;

[0010] The water pump is used to pump water into the housing, causing the housing to sink in the water, which in turn drives the water filtration and sampling components to sink.

[0011] The propeller assembly, mounted on the hull, is used to move the submerged hull in the water;

[0012] An air-inflating drainage assembly is used to inflate the housing to expel water, causing the housing to float and thus lifting the water filtration and sampling assembly for sampling; and

[0013] A rotating rod is mounted on a housing. A drive blade is installed at the bottom of the rotating rod and is disposed inside the housing. A cutting element is fixedly installed at the top of the rotating rod.

[0014] When the shell floats up, causing the water filtration and sampling assembly to float up, the driving blade drives the rotating rod to rotate synchronously under the driving force of the water flow inside the shell, so that the cutting part can cut and sample, and then the sample obtained by the cutting is acquired by the floating water filtration and sampling assembly.

[0015] Preferably, the inflatable drainage assembly includes:

[0016] A suction component is mounted on the housing. The air inlet of the suction component is connected to an air inlet pipe, and the air outlet of the suction component is connected to the inside of the housing.

[0017] A telescopic component is hingedly mounted on the housing. A hinge point is provided between the air intake pipe and the housing. The extended end of the telescopic component is hinged to the air intake pipe.

[0018] A drain pipe, at least one of which is provided and installed at the bottom of the housing, is equipped with a drain check valve.

[0019] Preferably, the intake pipe includes:

[0020] A movable tube is hinged to the housing, and the telescopic component connects the movable tube and the housing.

[0021] A flexible hose is provided, with one end of the movable tube connected to the air inlet of the suction unit via the flexible hose.

[0022] Preferably, at least one inflatable drainage assembly is provided, and a waterproof cover for preventing water from entering the suction component is also installed on the housing, and the air outlet of the suction component is also provided with an exhaust one-way valve.

[0023] Preferably, the rotating rod includes:

[0024] The main rod body is rotatably mounted inside the housing, and the drive vane is disposed on the main rod body;

[0025] A folded rod is fixed to the top of the main rod body, and a cutting component is rotatably mounted on the top of the folded rod. The cutting height of the cutting component is higher than the sampling height of the water filtration sampling assembly.

[0026] Preferably, the cutting element is rotatably mounted on the top of the folding rod.

[0027] Preferably, the water filtration sampling component includes:

[0028] A water filter frame is disposed on the top of the housing and its bottom is connected to the top surface of the housing by an elastic connector.

[0029] The sample container is disposed within the filter frame; and

[0030] A magnetic fastener is installed on the filter frame and magnetically engages with the sample container to fix the sample container in place.

[0031] The filter frame is coaxial with the main rod, the folded rod rotates around the axis of the filter frame, and the cutting element is located on the outer side of the filter frame.

[0032] Preferably, at least one protrusion is fixedly provided on the periphery of the water filtration sampling component, and a roller is rotatably mounted on the folded rod, with the protrusion positioned on the travel trajectory of the roller.

[0033] Preferably, the housing is provided with a groove that rotatably engages with the folded rod, and a ball bearing is provided in the groove.

[0034] The microbial sampler for environmental monitoring provided in this invention sample the aquatic environment by having the shell drive the water filtration sampling component to sink, move, and float. This method is simple to implement and can effectively utilize the water flow driving force generated by the drainage during the ascent to drive the cutting component to cut and collect the sample. The cut sample is then obtained through the water filtration sampling component, avoiding the defects of traditional sampling that are prone to contamination. This ensures the accuracy of the sampling research results. In addition, the linkage effect can shake off aquatic organisms adhering to the water filtration sampling component, reducing the impact on the sampling research, lowering the degree of contamination, and reducing the intensity of manual labor. Attached Figure Description

[0035] Figure 1 This is a main cross-sectional view of a microbial sampler for environmental monitoring provided in an embodiment of the present invention;

[0036] Figure 2 A top view of a microbial sampler for environmental monitoring provided in an embodiment of the present invention;

[0037] Figure 3 for Figure 1 Enlarged view of the inflatable drainage assembly and sampling assembly;

[0038] Figure 4 This is a schematic diagram of the working path of a microbial sampler used for environmental monitoring.

[0039] Figure 5 A perspective view related to the driving blades is provided for embodiments of the present invention;

[0040] Figure 6 This is a top view of the driving blade provided in an embodiment of the present invention;

[0041] Figure 7 This is a perspective view related to the driving blades in an embodiment of the present invention.

[0042] In the attached diagram: 1-Shell; 2-Propeller assembly; 3-Water pump; 4-Inflation and drainage assembly; 41-Suction assembly; 42-Air inlet pipe; 421-Moving pipe; 422-Hose; 43-Telescopic component; 44-Waterproof cover; 5-Drainage pipe; 51-Drainage check valve; 6-Rotating rod; 61-Main rod body; 62-Folded rod; 7-Drive rotor; 8-Cutting component; 9-Water filtration and sampling assembly; 901-Water filtration frame; 9011-Protrusion; 902-Sample container; 903-Magnetic fixing component; 10-Roller; 11-Elastic connector. Implementation

[0043] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the invention.

[0044] The specific implementation of the present invention will be described in detail below with reference to specific embodiments.

[0045] like Figures 1-7 The diagram shown is a structural diagram of a microbial sampler for environmental monitoring provided in an embodiment of the present invention, including a housing 1;

[0046] Water filtration sampling component 9 is installed on top of housing 1;

[0047] The water pumping component 3 is used to pump water into the housing 1, causing the housing 1 to sink in the water, thereby driving the water filtration and sampling component 9 to sink. The water pumping component 3 includes, but is not limited to, a water pump, a gear pump, etc.

[0048] Propeller assembly 2, mounted on housing 1, is used to move the submerged housing 1 in water. Propeller assembly 2 is existing technology and can be a fixed-pitch propeller or a variable-pitch propeller, which can be selected according to actual needs.

[0049] The air-inflation and drainage assembly 4 is used to inflate the housing 1 with air to expel the water inside the housing 1, causing the housing 1 to float and thus lifting the water filtration and sampling assembly 9 to float for sampling; and

[0050] Rotating rod 6 is mounted on housing 1. A driving blade 7 is mounted at the bottom of rotating rod 6 and is disposed inside housing 1. A cutting element 8 is fixedly mounted at the top of rotating rod 6.

[0051] When the housing 1 floats up, it causes the water filtration and sampling component 9 to float up as well. Under the action of the water flow driving force inside the housing 1, the driving blade 7 drives the rotating rod 6 to rotate synchronously, so that the cutting part 8 can cut and sample, and then the sample obtained by the cutting is acquired by the floating water filtration and sampling component 9.

[0052] It is worth noting that, in actual operation, a counterweight can be installed at the bottom of the housing 1 to enhance its operational stability.

[0053] In practical application, this embodiment pumps water into the housing 1 via the pump 3, causing the housing 1 to sink and thus sink the water filtration sampling component 9. The propeller assembly 2 then moves the sunken housing 1 in the water. Once it reaches below the target sampling point, the air-inflating and draining assembly 4 inflates the housing 1 to expel the water, causing the housing 1 to float and lift the water filtration sampling component 9 for sampling. This maximizes the integrity of the target sample and avoids the difficulty of directly sampling aquatic organisms from the water surface, a common problem in traditional aquatic organism sampling. Simultaneously, as the housing 1 floats and lifts the water filtration sampling component 9, the driving blade 7 rotates synchronously with the rotating rod 6 under the driving force of the water flow inside the housing 1. This allows the cutting component 8 to cut and sample, enabling the cutting of the desired aquatic organism sample. The cut sample is then obtained through the water filtration sampling component 9, avoiding the drawbacks of traditional sampling where floating objects adhere and make it difficult to obtain the desired ideal sample, and where non-target objects are easily removed along with the adhered objects, leading to sampling contamination. This ensures the accuracy of the sampling research results.

[0054] The structure of the inflatable drainage component 4 is not limited, as long as it can input gas into the shell 1 and expel water to make the shell 1 float. The inflatable drainage component 4 can be inflated using an air bladder or an air pump to inflate the shell 1. This embodiment provides a preferred form, such as... Figure 3 As shown, in a preferred embodiment of the present invention, the inflatable drainage assembly 4 includes:

[0055] A suction component 41 is mounted on the housing 1. The air inlet of the suction component 41 is connected to an air inlet pipe 42, and the air outlet of the suction component 41 is connected to the inside of the housing 1. The suction component 41 includes, but is not limited to, a negative pressure fan.

[0056] Telescopic component 43, which is hingedly mounted on the housing 1, has a hinge point between the air intake pipe 42 and the housing 1, and its extended end is hinged to the air intake pipe 42. The telescopic component 43 includes, but is not limited to, a telescopic rod; and

[0057] Drain pipe 5, at least one of which is provided and installed at the bottom of housing 1, and a drain one-way valve 51 is installed on the drain pipe 5.

[0058] In one embodiment, the intake pipe 42 includes:

[0059] The movable tube 421 is hinged to the housing 1, and the telescopic member 43 is connected between the movable tube 421 and the housing 1.

[0060] Hose 422, one end of the movable tube 421 is connected to the air inlet of the suction component 41 via hose 422.

[0061] In practical application, this embodiment utilizes the following method: when air inflation and drainage are required, the suction component 41 is activated, and the outer end of the air inlet pipe 42 is extended out of the water surface using the telescopic component 43. This allows air to be drawn from the water surface and inflated into the housing 1. The water inside the housing 1 is then discharged through the drain pipe 5. The drain check valve 51 prevents backflow of water, causing the housing 1 to rise gradually. Simultaneously, during air inflation and drainage, the water flow within the housing 1 generates a driving force on the drive blade 7, causing the rotating rod 6 and the drive blade 7 to rotate synchronously. This allows the cutting component 8 to perform cutting and sampling, and the resulting sample is then collected by the floating water filtration sampling component 9. This design fully utilizes the air inflation, drainage, rising sampling, and rising cutting techniques, resulting in a novel and highly practical design.

[0062] like Figures 1-2 As shown, in another preferred embodiment of the present invention, at least one inflatable drainage component 4 is provided, and a waterproof cover 44 for preventing water from entering the suction component 41 is also installed on the housing 1, and the air outlet of the suction component 41 is also provided with an exhaust one-way valve.

[0063] In practical applications, this embodiment is as follows: Figure 3 As shown, there are two inflatable drainage components 4. The inflatable drainage components 4 are arranged outside the water filtration sampling component 9 so that the inflatable drainage components 4 will not affect the sampling process. The waterproof cover 44 can provide waterproof protection for the suction component 41. The exhaust one-way valve can prevent the backflow of gas entering the housing 1. At the same time, it can also prevent water from being sucked back into the suction component 41. Of course, a one-way waterproof valve or a waterproof exhaust filter (such as a hydrophobic and breathable membrane) can also be installed in the movable tube 421.

[0064] The specific structure of the rotating rod 6 is not limited, as long as it can achieve rotation relative to the housing 1 under the action of water driving force and the driving blade 7. The rotating rod 6 can be a combination of a main rotating rod and a secondary transmission rod. The driving blade 7 is installed on the main rotating rod, and the secondary rotating rod is used to install the cutting part 8. The main rotating rod and the secondary rotating rod are connected by a transmission gear. Of course, it can also be a single rod body with the cutting part 8 installed. This embodiment provides a preferred form, such as... Figures 1-3 , Figure 6 and Figure 7 As shown, the rotating rod 6 includes:

[0065] Main rod 61, which is rotatably mounted inside housing 1, and drive blade 7 is disposed on main rod 61;

[0066] The folded rod 62 is fixed to the top of the main rod 61, and the cutting element 8 is rotatably installed on the top of the folded rod 62. The cutting height of the cutting element 8 is higher than the sampling height of the water filtration sampling assembly 9.

[0067] In practical application, the main rod 61 and the folded rod 62 are designed so that the folded rod 62 can rotate around the main rod 61 as the rotation center. The folded rod 62 drives the cutting piece 8 to cut the aquatic organisms. Since the cutting height of the cutting piece 8 is higher than the sampling height of the water filtration sampling component 9, the cutting can be performed before sampling when the shell 1 rises.

[0068] The specific structure of the water filtration sampling component 9 is not limited. The water filtration sampling component 9 can be an inverted mesh or a robotic arm, as long as it can sample aquatic organisms during the ascent of the shell 1. Figures 1-2 As shown, in another preferred embodiment of the present invention, the water filtration sampling component 9 includes:

[0069] A water filter frame 901 is disposed on the top of the housing 1 and its bottom is connected to the top surface of the housing 1 by an elastic connector 11.

[0070] Sample container 902, wherein the sample container 902 is disposed within the filter frame 901; and

[0071] A magnetic fastener 903 is installed on the filter frame 901. The magnetic fastener 903 is magnetically attracted to the sample container 902 and is used to fix the sample container 902.

[0072] The filter frame 901 is coaxial with the main rod 61, the folded rod 62 rotates around the axis of the filter frame 901, and the cutting piece 8 is located on the outer side of the filter frame 901.

[0073] In practical applications, this embodiment allows the water filtration sampling component 9 to be installed so that when the housing 1 rises, the sample obtained by cutting and sampling by the cutting component 8 can enter the sample container 902 inside the water filtration frame 901. As the water filtration sampling component 9 continues to rise with the housing 1, the water filtration frame 901 can filter out excess water. Of course, some water adhering to the sample will be retained, which can also be used for sampling and research. The sample container 902 can be disassembled and assembled by the magnetic fixing component 903, which facilitates multiple samplings.

[0074] like Figures 2-3 As shown, in another preferred embodiment of the present invention, at least one protrusion 9011 is fixedly provided on the periphery of the water filtration sampling component 9, and a roller 10 is rotatably mounted on the folded rod 62, with the protrusion 9011 arranged on the travel trajectory of the roller 10.

[0075] In practical applications, the protrusion 9011 is positioned on the travel trajectory of the roller 10, so that when the folding rod 62 rotates, it can drive the roller 10 to move synchronously. The protrusion 9011 provides a certain degree of obstruction to the roller 10. In this way, the water filtration sampling assembly 9 can vibrate to a certain extent under the combined action of the roller 10 and the elastic connector 11, which can shake off aquatic organisms that are naturally adhered or adhered to the outer wall of the water filter frame 901 after being cut by the cutter 8, reducing the impact on the sampling and research inside the sample container 902, reducing the degree of contamination, and at the same time facilitating the cleanliness of the water filter frame 901 and reducing the labor intensity of manual cleaning.

[0076] like Figure 3 As shown, in another preferred embodiment of the present invention, the housing 1 is provided with a groove (not shown in the figure) that rotatably engages with the folded rod 62, and a ball bearing (not shown in the figure) is provided in the groove.

[0077] In one embodiment, the folded rod 62 includes a horizontal part and a vertical part that are fixedly connected. The horizontal part is fixed to the top of the main rod body 61. The folded rod 62 is disposed in a groove opened in the shell wall of the housing 1. The top of the main rod body 61 extends into the groove. A sealing bearing is installed on the main rod body 61 at the connection position between the groove and the inside of the housing 1. In this way, the connection position between the folded rod 62 and the main rod body 61 can be sealed, thereby achieving a better effect of preventing air and water leakage inside the housing 1.

[0078] In practical applications, the groove and ball bearings in this embodiment can reduce the friction between the folded rod 62 and the housing 1, making the overall rolling of the rotating rod 6 smoother.

[0079] The above embodiments of the present invention provide a microbial sampler for environmental monitoring. A water pump 3 causes the housing 1 to sink in water, which in turn causes the water filtration and sampling assembly 9 to sink into the water. A propeller assembly 2 then moves the sinking housing 1 in the water to below the target sampling point. An air-inflating and draining assembly 4 inflates the housing 1 to expel the water inside, causing the housing 1 to float and thus lifting the water filtration and sampling assembly 9 for sampling. This ensures complete sampling and avoids the difficulty of directly sampling aquatic organisms from the water surface, a common problem in traditional aquatic biological sampling. Simultaneously, as the housing 1 floats and lifts the water filtration and sampling assembly 9, the driving vane 7, under the driving force of the water flow inside the housing 1, drives the rotating rod 6. Synchronous rotation enables the cutting component 8 to cut and sample, allowing for the cutting of the desired aquatic biological samples. The cut samples are then collected through the water filtration sampling component 9, avoiding the drawbacks of traditional sampling methods where floating debris adheres and makes it difficult to obtain the desired ideal sample, and where non-target objects are easily removed along with the debris, leading to sampling contamination. This ensures the accuracy of the sampling research results. At the same time, the linkage action shakes off naturally adhering aquatic organisms and those that have adhered to the outer wall of the water filtration frame 901 after being cut by the cutting component 8, reducing the impact on the sampling research inside the sample container 902, reducing contamination, and facilitating the cleanliness of the water filtration frame 901, thus reducing the labor intensity of manual cleaning.

[0080] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A microbial sampler containing an air-filled drainage assembly, characterized in that, include: case; The water filtration and sampling assembly is installed on the top of the housing; the water pump is used to pump water into the housing, causing the housing to sink in the water, which in turn drives the water filtration and sampling assembly to sink. The propeller assembly, mounted on the housing, is used to move the submerged housing in the water; the air-inflation and drainage assembly is used to inflate the housing with air to expel the water inside, causing the housing to float and thus lifting the water filtration and sampling assembly to float up for sampling. The rotating rod is mounted on the housing, with a drive vane installed at the bottom of the rotating rod and disposed inside the housing. A cutting component is fixedly installed at the top of the rotating rod. When the housing floats up, causing the water filtration and sampling assembly to float up, the drive vane drives the rotating rod to rotate synchronously under the action of the water flow driving force inside the housing, so that the cutting component can cut and sample, thereby allowing the sample obtained from the cutting to be acquired by the floating water filtration and sampling assembly. The inflatable drainage assembly includes: a suction component mounted on the housing, the suction component having an air inlet connected to an air inlet pipe and an air outlet communicating with the interior of the housing; a telescopic component hinged to the housing, the telescopic component having a hinge point between the air inlet pipe and the housing, and the extended end of the telescopic component hinged to the air inlet pipe; and a drain pipe, at least one of which is provided and installed at the bottom of the housing, and a drain one-way valve is installed on the drain pipe. The rotating rod includes: a main rod body, which is rotatably installed inside the housing, and the driving blade is disposed on the main rod body; a folded rod, which is fixed to the top of the main rod body, and the cutting component is rotatably installed on the top of the folded rod, the cutting height of the cutting component being higher than the sampling height of the water filtration sampling assembly; The rotating rod can be a combination of a main rotating rod and a secondary transmission rod. The main rotating rod is equipped with a drive blade, and the secondary rotating rod is used to install the cutting parts. The main rotating rod and the secondary rotating rod are connected by a transmission gear. The water filtration sampling assembly includes: a water filter frame, which is disposed on the top of the housing and its bottom is connected to the top surface of the housing via an elastic connector; a sample container, which is disposed inside the water filter frame; and a magnetic fastener, which is installed on the water filter frame and magnetically engages with the sample container; the water filter frame is coaxial with the main rod, the folded rod rotates about the axis of the water filter frame as the rotation center, and the cutting component is disposed on the outer side of the water filter frame; At least one protrusion is fixedly provided on the periphery of the water filtration sampling component, and a roller is rotatably mounted on the folded rod, with the protrusion positioned on the travel trajectory of the roller.

2. The microbial sampler containing an air-filled drainage component according to claim 1, characterized in that, The air intake pipe includes: a movable pipe, which is hinged to the housing, and the telescopic component is connected between the movable pipe and the housing; and a flexible hose, one end of which is connected to the air intake of the suction component.

3. The microbial sampler containing an air-filled drainage assembly according to claim 1 or 2, characterized in that, At least one inflatable drainage component is provided, and a waterproof cover for preventing water from entering the suction component is also installed on the housing. The air outlet of the suction component is also provided with an exhaust one-way valve.

4. The microbial sampler containing an air-filled drainage component according to claim 1, characterized in that, The housing is provided with a groove that rotatably engages with the folded rod, and a ball bearing is provided in the groove.

5. The application of the microbial sampler containing an air-filled drainage component according to any one of claims 1-4 in environmental monitoring.

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