An attractant for trypanosoma brucei

Abstract

The present application belongs to the technical field of insect attractant, and particularly relates to a kind of Chilo suppressalis attractant. A kind of Chilo suppressalis attractant composition, active ingredient is combined by cis-11-hexadecenyl aldehyde and cis-9-octadecenyl aldehyde or cis-13-octadecenyl aldehyde, and the mass ratio of the two is 1:1-10:1. The lure core of the present application is put into the trap, which can be used for monitoring the field occurrence dynamics of Chilo suppressalis, predicting its damage degree, guiding other control measures to be carried out in time, and has significant ecological and social benefits.

Description

Technical Field

[0001] This invention belongs to the field of insect attractant technology, specifically relating to a rice stem borer attractant and its application. Background Technology

[0002] The rice stem borer (Salix tricolor) is an insect belonging to the family Pyralidae in the order Lepidoptera. It has a single diet, feeding exclusively on rice. The larvae bore into the stems, causing damage during the tillering stage, resulting in dead hearts. From the booting to heading stage, it causes withered booted panicles and whiteheads. Infestation on other plants also creates damaged plants. "Dead hearts" and "whiteheads" are the main symptoms of rice plants after infestation.

[0003] Currently, the main method for controlling the rice stem borer is the use of chemical pesticides, but this leads to the overuse of pesticides, hinders the application of ecological control technologies, and causes a series of serious ecological problems.

[0004] To address the aforementioned ecological problems, sex pheromones and pheromone lures, made from insect sex pheromones, are a new type of green pest control technology that has been developed in recent years. They have the advantages of protecting natural enemies, restoring the ecosystem, having no adverse effects on the environment, and being non-toxic to humans and animals.

[0005] There is limited research on the sex pheromones of rice stem borer in the existing technology. The literature (Research on the sex pheromones of rice stem borer, Du Jiawei) discloses that the sex pheromones of rice stem borer are 16:AID, (Z)-9-16:AID, and (Z)-11-16:AID; and discloses that mixing the three components in a certain proportion has a good attraction effect.

[0006] Currently, there are no reports of using cis-11-hexadecenal in combination with cis-9-octadecenal or cis-13-octadecenal as an attractant for rice stem borer. Summary of the Invention

[0007] To address the problems existing in the prior art, the present invention provides the following technical solution:

[0008] A rice stem borer attractant, characterized in that the active ingredient is composed of cis-11-hexadecenal and cis-9-octadecenal or cis-13-octadecenal.

[0009] Preferably, the active ingredient is a combination of cis-11-hexadecenal and cis-9-octadecenal.

[0010] In the attractant, the mass ratio of cis-11-hexadecenal to cis-9-octadecenal or cis-13-octadecenal is 1:1 to 10:1.

[0011] Preferably, the mass ratio of cis-11-hexadecenal to cis-9-octadecenal or cis-13-octadecenal is 1:1 to 5:1.

[0012] Preferably, the mass ratio of cis-11-hexadecenal to cis-9-octadecenal or cis-13-octadecenal is 3:1.

[0013] This invention provides a attractant lure for the rice stem borer, wherein the attractant composition is used to prepare the lure, and each lure contains 1-200 μg of active ingredient, using redistilled n-hexane as the solvent. As a preferred embodiment, the lure also contains antioxidants and UV stabilizers.

[0014] The attractant composition and the attractant core provided by the present invention can be used to control the rice stem borer.

[0015] The attractant composition and the lure provided by this invention can be used for monitoring rice stem borer populations.

[0016] Compared with the prior art, the present invention has the following beneficial effects:

[0017] (1) When cis-11-hexadecenal is used in combination with cis-9-octadecenal or cis-13-octadecenal (when the content of cis-11-hexadecenal is high), the combination has an excellent attraction effect on the rice stem borer; especially when the mass ratio of cis-11-hexadecenal to cis-9-octadecenal is 3:1, the average number of insects trapped is 34.75.

[0018] (2) The attractant of the present invention is simple, safe and low in cost. It can be applied to the prediction, forecasting and control of rice stem borer and has good social, economic and ecological benefits. Detailed Implementation

[0019] The present invention will be further explained below with reference to specific embodiments.

[0020] Example 1 Field trials

[0021] 1. Overview of the test site

[0022] The experiment was conducted in the core rice-growing area of ​​Luodai Village, Basuo Town, Dongfang City, Hainan Province. The area has a contiguous rice planting area of ​​about 500 mu, the rice is well managed, and the soil is loam.

[0023] 2. Test Plan

[0024] 2.1 Test Materials

[0025] cis-11-hexadecenal (A), cis-9-octadecenal (B), cis-13-octadecenal (C); Preparation of lure core: Use organosilicone lure cores, add 20 μg of uniformly mixed attractant to each lure core, and use redistilled n-hexane as solvent.

[0026] 2.2 Experimental Design

[0027] Select flat paddy fields with consistent varieties, cultivation methods, pesticide application, and fertilization as experimental fields, and select fields with moderate to high incidence levels based on the local historical occurrence of rice stem borers.

[0028] The traps for different treatments were randomly arranged in a checkerboard pattern, 10 meters away from the edge of the field. In the early stage of the emergence of the first generation of rice stem borers, the traps were evenly placed on the edge of the rice field in a counterclockwise direction, so that the insect inlet of the trap was level with the top of the rice.

[0029] 2.3 Test Time

[0030] Test date: March 31, 2020;

[0031] The trap was adjusted and the lure core was replaced on April 15, 2020.

[0032] Test results date: April 26, 2020

[0033] 2.3 Experimental Investigation

[0034] The experiment included treatments and controls, and was repeated four times, with each plot randomly arranged. Surveys began the day after installation of the traps for each treatment, with daily records kept of the number of rice stem borers trapped in each treatment (the traps were cleaned of any remaining rice stem borer remains after each survey) until the end of the experiment.

[0035] 2.4 Test Results

[0036] The results are shown in Table 1.

[0037] Table 1. Trapping volume of each treatment trap

[0038]

[0039] Table 1 shows that the attraction effects of cis-11-hexadecenal, cis-9-octadecenal, and cis-13-octadecenal on the rice stem borer were low when used alone, with average trapping rates of 6.5, 3.25, and 2.25 individuals, respectively. When cis-11-hexadecenal was used in combination with cis-9-octadecenal or cis-13-octadecenal (when the cis-11-hexadecenal content was higher), the attraction effect of the combination on the rice stem borer was significantly improved; in particular, when the mass ratio of cis-11-hexadecenal to cis-9-octadecenal was 3:1, the average trapping rate was 34.75 individuals.

[0040] Example 2

[0041] As an embodiment of the present invention, the total amount of active ingredients in the rice stem borer attractant is 40 mg, which is composed of the following components in parts by weight: 3 parts of cis-11-hexadecenal and 1 part of cis-9-octadecenal.

[0042] Example 3

[0043] As an embodiment of the present invention, the total amount of active ingredients in the rice stem borer attractant is 50 mg, which is composed of the following components in parts by weight: 4 parts of cis-11-hexadecenal and 1 part of cis-9-octadecenal.

[0044] Example 4

[0045] As an embodiment of the present invention, the total amount of active ingredients in the rice stem borer attractant is 20 mg, which is composed of the following components in parts by weight: 1 part of cis-11-hexadecenal and 1 part of cis-9-octadecenal.

[0046] Example 5

[0047] As an embodiment of the present invention, the total amount of active ingredients in the rice stem borer attractant is 60 mg, which is composed of the following components in parts by weight: 5 parts of cis-11-hexadecenal and 1 part of cis-13-octadecenal.

[0048] Example 6

[0049] As an embodiment of the present invention, the total amount of active ingredients in the rice stem borer attractant is 50 mg, which is composed of the following components in parts by weight: 1 part of cis-11-hexadecenal and 1 part of cis-13-octadecenal.

[0050] Example 7

[0051] As an embodiment of the present invention, the total amount of active ingredients in the rice stem borer attractant is 400 mg, which is composed of the following components in parts by weight: 3 parts of cis-11-hexadecenal and 1 part of cis-13-octadecenal.

[0052] This invention provides the application of a rice stem borer attractant in the control and / or monitoring of the rice stem borer. The attractant provided by this invention can be used in various forms. It can be prepared as a lure core, or the composition can be loaded into a polyethylene tube sealed at both ends and fixed in the center of a sticky trap, allowing the attractant to be slowly released to trap adult rice stem borers.

[0053] The rice stem borer attractant provided by this invention can be used for rice stem borer infestation monitoring and mass trapping, which is of great significance for integrated management of the rice stem borer. This attractant is highly specific, does not induce resistance, is harmless to natural enemies, non-toxic to humans and livestock, and is environmentally friendly; it is also highly resistant to adverse conditions, resistant to wind, rain, and sun exposure; it is simple to prepare, easy to use, has a long-lasting effect, and is feasible under various conditions. When used for infestation monitoring, this attractant has high accuracy, and the number of insects trapped can accurately predict the dynamics of rice stem borer occurrence; it can also be used for mass trapping, significantly reducing the number of rice stem borers in the field and lowering the population density of offspring.

[0054] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit it.

Claims

1. Use of a yellow rice borer attractant lure for controlling yellow rice borer, characterized in that, The effective component is composed of cis-11-hexadecenyl aldehyde and cis-13-octadecenyl aldehyde, the mass ratio of cis-11-hexadecenyl aldehyde and cis-13-octadecenyl aldehyde is 3:1; the single lure core contains 1-200 μg of the effective component, and the solvent is heavy distilled n-hexane.

2. Use of a yellow rice borer attractant lure for yellow rice borer population monitoring, characterized in that, The effective component is composed of cis-11-hexadecenyl aldehyde and cis-13-octadecenyl aldehyde, the mass ratio of cis-11-hexadecenyl aldehyde and cis-13-octadecenyl aldehyde is 3:1; the single lure core contains 1-200 μg of the effective component, and the solvent is heavy distilled n-hexane.

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