A method, device, equipment and storage medium for screening intestinal flora of donors

Abstract

The present invention relates to a method, device, equipment and storage medium for screening intestinal flora of a donor, the method comprising: based on preset rules, evaluating the initial donors to screen out qualified donors that meet the requirements; processing and analyzing the fecal samples of the qualified donors for a preset number of times to obtain initial intestinal flora data and construct a donor database; using the subsequent intestinal flora data of the qualified donors as a test set, calculating the weighted offset of the intestinal flora of the test set; comparing the weighted offset of the intestinal flora of the test set with the donor database, and screening the subsequent intestinal flora of the qualified donors. The present invention first screens the qualified donors, then determines the initial intestinal flora data based on the feces of the qualified donors and establishes a donor database, analyzes the subsequent feces of the qualified donors to extract the subsequent intestinal flora data, compares with the donor database, and screens the subsequent intestinal flora of the qualified donors to ensure the uniformity of the subsequent intestinal flora of the qualified donors.

Description

Technical Field

[0001] The present invention relates to the field of intestinal microbial technology, and in particular to a method, device, equipment and storage medium for screening donor intestinal flora. Background Art

[0002] Intestinal flora, as a general term for the microbial community living in the human intestine, has been one of the most popular research focuses in the fields of microbiology, medicine, and genetics in recent years. Intestinal flora transplantation (FMT) is defined as transplanting the functional flora in the feces of healthy people into the intestines of patients to rebuild new intestinal flora.

[0003] In the prior art, in order to control the uniformity of the flora, the screening methods for intestinal flora are to model the differential flora for a single disease, and evaluate and predict the flora based on the model established by the differential flora, so as to manage the intestinal flora and screen out the flora that meets the requirements.

[0004] However, since the intestinal flora of the donor may change due to changes in lifestyle or living environment or taking antibiotics, the output results of the methods in the existing technology are not comparable between projects, making it impossible to effectively integrate the analysis results of multiple projects and it is difficult to ensure the uniformity of the intestinal flora. Summary of the invention

[0005] In view of this, it is necessary to provide a method, device, equipment and storage medium for screening donor intestinal flora to solve the problem in the prior art that the donor's intestinal flora may change due to changes in living habits or living environment or taking antibiotics, resulting in the inability to effectively integrate multiple project analysis results and difficulty in ensuring the uniformity of the intestinal flora.

[0006] In order to achieve the above technical objectives, the present invention adopts the following technical solutions:

[0007] In a first aspect, the present invention provides a method for screening a donor intestinal flora, comprising:

[0008] Based on preset rules, the initial donors are evaluated and qualified donors who meet the requirements are selected;

[0009] Process and analyze the fecal samples of qualified donors for a preset number of times to obtain initial intestinal flora data and build a donor database;

[0010] The subsequent intestinal flora data of qualified donors are used as a test set, and the weighted offset of the intestinal flora of the test set is calculated;

[0011] The weighted offset of the intestinal flora of the test set was compared with the donor database to screen the subsequent intestinal flora of qualified donors.

[0012] In some possible implementations, the intestinal flora data includes: intestinal flora ASV and intestinal flora abundance value; the fecal samples of a preset number of qualified donors are processed and analyzed to obtain initial intestinal flora data and construct a donor database, including:

[0013] DNA was extracted from a preset number of stool samples from qualified donors, and the DNA was sequenced to obtain the original sequence;

[0014] The original sequences were preprocessed, species annotated, and abundance analyzed to obtain intestinal flora markers and intestinal flora abundance values;

[0015] The intestinal flora markers were processed to determine the intestinal flora ASV;

[0016] A donor database was established using preset ranked gut microbiota ASVs and gut microbiota abundance values.

[0017] In some possible implementations, the subsequent intestinal flora data of the qualified donor is used as a test set, and the weighted offset of the intestinal flora of the test set is calculated, including:

[0018] Calculate the weight coefficient of the preset ranked intestinal flora according to the test set and the initial intestinal flora data;

[0019] The bacterial flora offset of the test set is calculated based on the intestinal flora abundance value of the test set and the donor database;

[0020] The weighted offset of the intestinal flora of the test set is determined according to the weight coefficient and the flora offset of the test set.

[0021] In some possible implementations, the weight coefficient of the intestinal flora with a preset ranking is calculated according to the test set and the initial intestinal flora data, including:

[0022] Arranging the intestinal flora abundance values ​​of the test set and the intestinal flora abundance values ​​of the donor database according to a first preset order to obtain the rank of the intestinal flora;

[0023] The ranks of the same intestinal flora abundance values ​​are updated to the average of the ranks of the corresponding intestinal flora;

[0024] The ranks of the preset ranked intestinal flora are sorted according to a second preset order to obtain a weight coefficient of the intestinal flora.

[0025] In some possible implementations, determining the flora offset of the test set according to the intestinal flora abundance value of the test set and the donor database includes:

[0026] When the intestinal flora abundance value of the test set is higher than the intestinal flora abundance value in the donor database, the flora offset of the test set is set according to the first offset rule;

[0027] When the intestinal flora abundance value of the test set is lower than the intestinal flora abundance value in the donor database, the flora offset of the test set is set according to the second offset rule.

[0028] In some possible implementations, when the intestinal flora abundance value of the test set is higher than the intestinal flora abundance value in the donor database, the flora offset of the test set is set according to the first offset rule, including:

[0029] When the intestinal flora abundance value of the test set is higher than the intestinal flora abundance value of the first proportion in the donor database, the flora offset is the first preset value;

[0030] When the intestinal flora abundance value of the test set is higher than the intestinal flora abundance value of the second proportion in the donor database and lower than the intestinal flora abundance value of the first proportion in the donor database, the flora offset is the second preset value;

[0031] When the intestinal flora abundance value of the test set is higher than the intestinal flora abundance value of the third proportion in the donor database and lower than the intestinal flora abundance value of the second proportion in the donor database, the flora offset is the third preset value;

[0032] When the intestinal flora abundance value of the test set is higher than the intestinal flora abundance value of the fourth proportion in the donor database and lower than the intestinal flora abundance value of the third proportion in the donor database, the flora offset is a fourth preset value.

[0033] In some possible implementations, when the intestinal flora abundance value of the test set is lower than the intestinal flora abundance value in the donor database, the flora offset of the test set is set according to the second offset rule, including:

[0034] When the intestinal flora abundance value of the test set is lower than the intestinal flora abundance value of the first proportion in the donor database, the flora offset is the first preset value;

[0035] When the intestinal flora abundance value of the test set is lower than the intestinal flora abundance value of the second proportion in the donor database and higher than the intestinal flora abundance value of the first proportion in the donor database, the flora offset is the second preset value;

[0036] When the intestinal flora abundance value of the test set is lower than the intestinal flora abundance value of the third proportion in the donor database and higher than the intestinal flora abundance value of the second proportion in the donor database, the flora offset is the third preset value;

[0037] When the intestinal flora abundance value of the test set is lower than the intestinal flora abundance value of the fourth percentage in the donor database and higher than the intestinal flora abundance value of the third percentage in the donor database, the flora offset is a fourth preset value. In a second aspect, the present invention also provides a screening device for donor intestinal flora, comprising:

[0038] The donor screening module is used to evaluate the initial donors and select qualified donors that meet the requirements based on preset rules;

[0039] A construction module is used to process and analyze a preset number of stool samples from qualified donors to obtain initial intestinal flora data and construct a donor database;

[0040] A calculation module, used to use the subsequent intestinal flora data of the qualified donor as a test set and calculate the weighted offset of the intestinal flora of the test set;

[0041] The microbiota screening module is used to compare the weighted offset of the intestinal microbiota of the test set with the donor database and screen the subsequent intestinal microbiota of qualified donors.

[0042] In a third aspect, the present invention also provides a screening device for donor intestinal flora, comprising a memory and a processor, wherein:

[0043] Memory, used to store programs;

[0044] The processor is coupled to the memory and is used to execute the program stored in the memory to implement the steps in the method for screening the donor intestinal flora in any of the above implementations.

[0045] In a fourth aspect, the present invention further provides a computer-readable storage medium for storing computer-readable programs or instructions, which, when executed by a processor, can implement the steps in the method for screening the donor intestinal flora in any of the above-mentioned implementations.

[0046] The beneficial effect of adopting the above embodiment is: the present invention relates to a method, device, equipment and storage medium for screening intestinal flora of a donor, the method comprising: based on preset rules, evaluating the initial donor to screen out qualified donors that meet the requirements; processing and analyzing the fecal samples of the qualified donors for a preset number of times to obtain initial intestinal flora data and construct a donor database; using the subsequent intestinal flora data of the qualified donor as a test set, calculating the weighted offset of the intestinal flora of the test set; comparing the weighted offset of the intestinal flora of the test set with the donor database, and screening the subsequent intestinal flora of the qualified donor. The present invention first screens out qualified donors, then determines the initial intestinal flora data of the qualified donors according to the feces of the qualified donors and establishes a donor database based on this, then analyzes the subsequent feces of the qualified donors to extract the subsequent intestinal flora data, and compares the weighted offset with the donor database by calculating the weighted offset, thereby screening the subsequent intestinal flora of the qualified donors, and when the subsequent intestinal flora of the qualified donors changes, the unqualified intestinal flora can be timely eliminated, thereby ensuring the uniformity of the subsequent intestinal flora of the qualified donors. BRIEF DESCRIPTION OF THE DRAWINGS

[0047] Figure 1 A schematic diagram of a process of an embodiment of a method for screening donor intestinal flora provided by the present invention;

[0048] Figure 2 The present invention provides Figure 1 A schematic diagram of a flow chart of an embodiment of step S102;

[0049] Figure 3 The present invention provides Figure 1 A schematic flow chart of an embodiment of step S103;

[0050] Figure 4 The present invention provides Figure 3 A flowchart of an embodiment of step S301;

[0051] Figure 5 A schematic diagram of the distribution of key bacterial groups according to an embodiment of the present invention;

[0052] Figure 6 A schematic structural diagram of an embodiment of a screening device for donor intestinal flora provided by the present invention;

[0053] Figure 7 A schematic diagram of the structure of a device for screening donor intestinal flora provided in an embodiment of the present invention. DETAILED DESCRIPTION

[0054] The preferred embodiments of the present invention are described in detail below in conjunction with the accompanying drawings, wherein the accompanying drawings constitute a part of this application and are used together with the embodiments of the present invention to illustrate the principles of the present invention, but are not used to limit the scope of the present invention.

[0055] In the description of the present application, “plurality” means two or more than two, unless otherwise clearly and specifically defined.

[0056] Before describing the embodiments of the present invention, the following relevant terms are explained:

[0057] Donor: People who provide healthy intestinal flora;

[0058] Homogeneity: refers to the consistency of the intestinal flora provided by qualified donors and the intestinal flora in the donor database in terms of species;

[0059] DNA: refers to deoxyribonucleic acid, which is the basic molecule that constitutes the genetic information of organisms;

[0060] ASV: refers to the percentage content of each bacterial group in the intestinal flora;

[0061] OTU: Taxonomic operational unit, is the same symbol artificially set for a certain taxonomic unit (strain, species, genus, grouping, etc.) in order to facilitate analysis in phylogenetic studies or population genetics studies.

[0062] Reference to an "embodiment" herein means that a particular feature, structure, or characteristic described in conjunction with the embodiment may be included in at least one embodiment of the present invention. The appearance of the phrase in various places in the specification does not necessarily refer to the same embodiment, nor is it an independent or alternative embodiment that is mutually exclusive with other embodiments. It is explicitly and implicitly understood by those skilled in the art that the embodiments described herein may be combined with other embodiments.

[0063] The present invention provides a method, device, equipment and storage medium for screening donor intestinal flora, which are respectively described below.

[0064] See also Figure 1 , Figure 1 This is a flow chart of an embodiment of a method for screening a donor intestinal flora provided by the present invention. A specific embodiment of the present invention discloses a method for screening a donor intestinal flora, comprising:

[0065] S101. Based on preset rules, the initial donors are evaluated and qualified donors that meet the requirements are selected;

[0066] S102, processing and analyzing the fecal samples of the qualified donors for a preset number of times to obtain initial intestinal flora data and construct a donor database;

[0067] S103, using the subsequent intestinal flora data of the qualified donor as a test set, and calculating the weighted offset of the intestinal flora of the test set;

[0068] S104, comparing the weighted offset of the intestinal flora of the test set with the donor database, and screening subsequent intestinal flora of qualified donors.

[0069] In the above embodiments, the quality of the intestinal flora provided by the donor is related to various factors of the donor. In order to ensure that the intestinal flora provided by the donor meets the requirements, professional scale evaluation and clinical evaluation are required for the initial donors to screen out donors who can provide intestinal flora that meets the requirements. Such donors are qualified donors.

[0070] The preset number of stool samples are the first 10 qualified stool samples of the qualified donor. The initial intestinal flora data obtained by analyzing and processing these 10 qualified stool samples are the reference values ​​just provided for the qualified donors. A donor database is constructed based on this, and the intestinal flora subsequently provided by the qualified donors can be screened.

[0071] All subsequent intestinal flora data provided by qualified donors can be regarded as test sets, and the weighted offset of the intestinal flora of the test set is calculated. The intestinal flora of the screened qualified donors may change due to their own changes in the future. The weighted offset of the intestinal flora of the test set is used to measure whether the intestinal flora provided by the qualified donors subsequently changes.

[0072] Using the donor database as the standard, the weighted offset of the intestinal flora of the test set is compared. If the weighted offset exceeds the set threshold, it is considered that the intestinal flora of the test set has undergone a significant change, and the intestinal flora provided by the donor needs to be eliminated. In this way, the subsequent screening of the intestinal flora of qualified donors is completed, ensuring the uniformity of the intestinal flora subsequently provided by qualified donors.

[0073] Compared with the prior art, the present embodiment provides a method for screening the intestinal flora of a donor, the method comprising: based on a preset rule, evaluating the initial donor to screen out qualified donors that meet the requirements; processing and analyzing the fecal samples of the qualified donors for a preset number of times to obtain initial intestinal flora data and construct a donor database; using the subsequent intestinal flora data of the qualified donor as a test set, calculating the weighted offset of the intestinal flora of the test set; comparing the weighted offset of the intestinal flora of the test set with the donor database, and screening the subsequent intestinal flora of the qualified donor. The present invention first screens out the qualified donors, then determines the initial intestinal flora data of the qualified donors based on the feces of the qualified donors and establishes a donor database based on this, then analyzes the subsequent feces of the qualified donors to extract the subsequent intestinal flora data, and compares the weighted offset with the donor database by calculating the weighted offset, thereby screening the subsequent intestinal flora of the qualified donors, and when the subsequent intestinal flora of the qualified donors changes, the unqualified intestinal flora can be eliminated in time, thereby ensuring the uniformity of the subsequent intestinal flora of the qualified donors.

[0074] After determining the qualified donor, in order to screen the intestinal flora provided by the qualified donor and ensure the uniformity of the intestinal flora provided subsequently, a donor database is constructed based on the initial intestinal flora provided by the qualified donor as the basis for screening. Figure 2 , Figure 2 The present invention provides Figure 1 Schematic diagram of a flow chart of an embodiment of step S102 in the present invention, in some embodiments of the present invention, the intestinal flora data includes: intestinal flora ASV and intestinal flora abundance value; the fecal samples of the qualified donors for a preset number of times are processed and analyzed to obtain the initial intestinal flora data and construct the donor database, including:

[0075] S201, extracting DNA from a preset number of stool samples of a qualified donor, and sequencing the DNA to obtain an original sequence;

[0076] S202, preprocessing, species annotation and abundance analysis of the original sequence to obtain intestinal flora markers and intestinal flora abundance values;

[0077] S203, processing the intestinal flora marker to determine the intestinal flora ASV;

[0078] S204. Establish a donor database using preset ranked intestinal flora ASVs and intestinal flora abundance values.

[0079] In the above embodiment, the preset number of fecal samples is the first 10 provided by qualified donors, from which DNA data is extracted, the V3-V4 region of 16S ribosomal DNA is amplified by polymerase chain reaction, and the original sequence is obtained by high-throughput sequencing using a second-generation sequencing platform, and a qualified original sequence can be extracted.

[0080] It is understandable that extracting DNA data from the first 10 stool samples provided by a qualified donor can be achieved by existing technology, and the present invention does not need to elaborate on this.

[0081] Preprocessing of the original sequence includes but is not limited to splicing, filtering, clustering or denoising of the original sequence to remove interference information, and then performing species annotation and abundance analysis on the preprocessed original sequence to reveal the species composition of the sample, obtain OUT (intestinal flora marker) and intestinal flora abundance value, and divide all sequences into OTUs according to different similarity levels, and each OTU corresponds to a representative sequence.

[0082] After removing the erroneous sequences, the Identity standard is set to 100% and then clustering is performed (OTU is set to 97%), which is the intestinal flora ASV. It can be understood that the intestinal flora contains a variety of different flora, and the intestinal flora ASV can be used to characterize the proportion of the main flora in the intestinal flora.

[0083] The preset ranking refers to the top 10 ASV rankings from high to low. A donor database is established based on the intestinal flora ASV and intestinal flora abundance values ​​that pass the preset rankings to store the initial qualified data of qualified donors. This serves as a basis for judging the subsequent changes in the donor's intestinal flora, and the intestinal flora subsequently provided is screened in turn to ensure the uniformity of the intestinal flora.

[0084] See also Figure 3 , Figure 3 The present invention provides Figure 1 FIG. 1 is a flow chart of an embodiment of step S103 in FIG. 1 . In some embodiments of the present invention, the subsequent intestinal flora data of the qualified donor is used as a test set, and the weighted offset of the intestinal flora of the test set is calculated, including:

[0085] S301, calculating the weight coefficient of the intestinal flora of a preset ranking according to the test set and the initial intestinal flora data;

[0086] S302, calculating the flora offset of the test set according to the intestinal flora abundance value of the test set and the donor database;

[0087] S303: Determine a weighted offset of the intestinal flora of the test set according to the weight coefficient and the flora offset of the test set.

[0088] In the above embodiment, due to the diversity of flora types in the intestinal flora, the proportions of different flora are generally different. Therefore, different intestinal flora need to be assigned different weights. The weight coefficient of the preset ranked intestinal flora is calculated through the test set and the initial intestinal flora data to represent the proportion of the intestinal flora.

[0089] By comparing the abundance values ​​of the intestinal flora of the test set with the donor database, the offset of the intestinal flora of the test set can be determined, reflecting the initial changes in the intestinal flora of the test set relative to the donor database.

[0090] In order to accurately determine the changes in the intestinal flora of the test set, the weighted offset of the intestinal flora of the test set was determined by the weight coefficient and the flora offset of the test set, which reflects the changes in the main flora of the intestinal flora of the test set relative to the donor database.

[0091] See also Figure 4 , Figure 4 The present invention provides Figure 3 In some embodiments of the present invention, the weight coefficient of the intestinal flora of the preset ranking is calculated according to the test set and the initial intestinal flora data, including:

[0092] S401, arranging the intestinal flora abundance values ​​of the test set and the intestinal flora abundance values ​​of the donor database according to a first preset order to obtain the rank of the intestinal flora;

[0093] S402, updating the ranks of the intestinal flora with the same abundance value to the average of the ranks of the corresponding intestinal flora;

[0094] S403, sorting the ranks of the preset ranked intestinal flora according to a second preset order to obtain a weight coefficient of the intestinal flora.

[0095] In the above embodiment, to calculate the weight coefficient of the intestinal flora, it is necessary to study the specific flora. First, the abundance values ​​of the specific flora in the intestinal flora abundance values ​​in the donor database and the test set are arranged from large to small (first preset order) according to the abundance value to obtain the rank of each intestinal flora, which reflects the ranking of the proportion of each flora in the intestinal flora.

[0096] However, there may be intestinal flora with the same abundance values, which will result in the same rank of the flora. Therefore, for these flora, the ranks of the flora with the same intestinal flora abundance values ​​will be updated to the average of the ranks of the corresponding intestinal flora, thereby determining their final ranks.

[0097] The top 10 ranked microbiota with a preset ranking are sorted from high to low (a second preset order), and the weight coefficients of the microbiota are allocated according to the second preset order of the rank. In the present invention, the weight coefficients of the top 10 microbiota ranked from high to low are allocated from 10 to 1 in sequence, thereby determining the top 10 microbiota with the largest number in the intestinal microbiota.

[0098] In some embodiments of the present invention, determining the flora offset of the test set according to the intestinal flora abundance value of the test set and the donor database includes:

[0099] When the intestinal flora abundance value of the test set is higher than the intestinal flora abundance value in the donor database, the flora offset of the test set is set according to the first offset rule;

[0100] When the intestinal flora abundance value of the test set is lower than the intestinal flora abundance value in the donor database, the flora offset of the test set is set according to the second offset rule.

[0101] In the above embodiment, the intestinal flora subsequently provided by the qualified donor may change compared with the donor database. This change may be that some types of intestinal flora increase or decrease. For the increase and decrease of intestinal flora, it is necessary to separately determine the flora offset. Therefore, the first offset rule and the second offset rule are used to handle the increase and decrease of intestinal flora respectively.

[0102] In some embodiments of the present invention, when the intestinal flora abundance value of the test set is higher than the intestinal flora abundance value in the donor database, the flora offset of the test set is set according to the first offset rule, including:

[0103] When the intestinal flora abundance value of the test set is higher than the intestinal flora abundance value of the first proportion in the donor database, the flora offset is the first preset value;

[0104] When the intestinal flora abundance value of the test set is higher than the intestinal flora abundance value of the second proportion in the donor database and lower than the intestinal flora abundance value of the first proportion in the donor database, the flora offset is the second preset value;

[0105] When the intestinal flora abundance value of the test set is higher than the intestinal flora abundance value of the third proportion in the donor database and lower than the intestinal flora abundance value of the second proportion in the donor database, the flora offset is the third preset value;

[0106] When the intestinal flora abundance value of the test set is higher than the intestinal flora abundance value of the fourth proportion in the donor database and lower than the intestinal flora abundance value of the third proportion in the donor database, the flora offset is a fourth preset value.

[0107] In some embodiments of the present invention, when the intestinal flora abundance value of the test set is lower than the intestinal flora abundance value in the donor database, the flora offset of the test set is set according to the second offset rule, including:

[0108] When the intestinal flora abundance value of the test set is lower than the intestinal flora abundance value of the first proportion in the donor database, the flora offset is the first preset value;

[0109] When the intestinal flora abundance value of the test set is lower than the intestinal flora abundance value of the second proportion in the donor database and higher than the intestinal flora abundance value of the first proportion in the donor database, the flora offset is the second preset value;

[0110] When the intestinal flora abundance value of the test set is lower than the intestinal flora abundance value of the third proportion in the donor database and higher than the intestinal flora abundance value of the second proportion in the donor database, the flora offset is the third preset value;

[0111] When the intestinal flora abundance value of the test set is lower than the intestinal flora abundance value of the fourth proportion in the donor database and higher than the intestinal flora abundance value of the third proportion in the donor database, the flora offset is a fourth preset value.

[0112] In the above embodiment, as a preferred embodiment, the first proportion in the present invention is 95%, the second proportion is 90%, the third proportion is 85%, and the fourth proportion is 80%. The first preset value is 4, the second preset value is 3, the third preset value is 2, and the fourth preset value is 1. The flora offset is set according to the ratio of the intestinal flora abundance value of the test set and the intestinal flora abundance value in the donor database. When the intestinal flora abundance value of the test set is not in the above proportion, the flora offset is set to zero. The flora offset of the intestinal flora of the test set is preliminarily set by the above method, so as to understand the preliminary changes of the intestinal flora.

[0113] It is understandable that the first proportion, the second proportion, the third proportion, the fourth proportion and the first preset value, the second preset value, the third preset value, and the fourth preset value can be adjusted according to the actual needs of use, and the present invention does not impose further restrictions on this. In some embodiments of the present invention, the weighted offset of the intestinal flora of the test set is calculated according to the weight coefficient and the flora offset, including: multiplying the weight coefficient and the flora offset to obtain the weighted offset of the intestinal flora of the test set.

[0114] In the above embodiment, for each donor, it is necessary to calculate the weighted offset of the top 10 flora in terms of intestinal flora content in the test set. The weighted offset is obtained by multiplying the weight coefficient and the flora offset, so as to understand the changes of each major flora of each donor relative to the initial data, that is, the donor database.

[0115] In some embodiments of the present invention, the weighted offset of the intestinal flora of the test set is compared with the donor database to screen the subsequent intestinal flora of qualified donors, including:

[0116] The baseline weighted offset of the gut microbiota was calculated based on the donor database;

[0117] If the weighted offset of the intestinal flora in the test set exceeds the preset threshold of the baseline weighted offset, the current donor is eliminated.

[0118] In the above embodiment, the preset threshold is 75%, and the weighted offset is calculated for each qualified donor based on the first 10 qualified feces (donor database), and the weighted offset subsequently calculated for the donor is compared with the weighted offsets of these 10 times (baseline weighted offsets). As a preferred embodiment, in the present invention, when the weighted offset of the donor is greater than 75% of the baseline weighted offset, it means that the proportion of the intestinal flora of the donor has changed significantly, and the intestinal flora is not suitable for use, so the donor needs to be eliminated.

[0119] An embodiment provided by the present invention screened 68,125 donors, and finally screened out 109 qualified donors, with a pass rate of 1.6‰. The low donor pass rate proves the stringency of this screening scheme. The present invention randomly selected 4 screened qualified donors for 16SrRNA sequencing analysis of intestinal flora, and combined the samples of each donor at different time points as a group, and used the binary jaccard distance coefficient as an intra-group stability evaluation index. The donor group screened by this scheme is stable (Donor1 / Donor4), and the intra-group difference is smaller than the inter-group difference. And as the cycle of collecting donor fecal samples increases, there are differences between each donor sample, but they fluctuate within a stable range, and the donor's sample points are clustered together with a low degree of discreteness, from which it can be judged that the donor has good stability and uniformity (Donor1 / Donor4).

[0120] Another embodiment provided by the present invention performs statistical analysis on the top ten bacterial communities of qualified donors tested in the first ten times, see Figure 5 , Figure 5 A schematic diagram of the distribution of key bacterial groups according to an embodiment of the present invention is provided. Figure 5 Finally, the concentrated distribution areas (upper quartile and lower quartile) of the top ten bacterial communities in qualified donors were determined.

[0121] In order to better implement the screening method of the donor intestinal flora in the embodiment of the present invention, based on the screening method of the donor intestinal flora, correspondingly, please refer to Figure 6 , Figure 6 This is a schematic structural diagram of an embodiment of a screening device for a donor intestinal flora provided by the present invention. The embodiment of the present invention provides a screening device 600 for a donor intestinal flora, comprising:

[0122] The donor screening module 610 is used to evaluate the initial donors and screen out qualified donors that meet the requirements based on preset rules;

[0123] A construction module 620 is used to process and analyze a preset number of stool samples from qualified donors to obtain initial intestinal flora data and construct a donor database;

[0124] A calculation module 630 is used to use the subsequent intestinal flora data of the qualified donor as a test set and calculate the weighted offset of the intestinal flora of the test set;

[0125] The flora screening module 640 is used to compare the weighted offset of the intestinal flora of the test set with the donor database to screen the subsequent intestinal flora of qualified donors.

[0126] It should be noted here that: the device 600 provided in the above embodiment can implement the technical solutions described in the above method embodiments. The specific implementation principles of the above modules or units can refer to the corresponding contents in the above method embodiments, which will not be repeated here.

[0127] See also Figure 7 , Figure 7 A schematic diagram of the structure of a screening device for donor intestinal flora provided by an embodiment of the present invention. Based on the above screening method for donor intestinal flora, the present invention also provides a screening device for donor intestinal flora, which can be a computing device such as a mobile terminal, a desktop computer, a notebook, a PDA, and a server. The screening device for donor intestinal flora includes a processor 710, a memory 720, and a display 730. Figure 7 Only some components of the donor intestinal flora screening device are shown, but it should be understood that it is not required to implement all of the shown components, and more or fewer components may be implemented instead.

[0128] In some embodiments, the memory 720 may be an internal storage unit of the screening device for the donor intestinal flora, such as a hard disk or memory of the screening device for the donor intestinal flora. In other embodiments, the memory 720 may also be an external storage device of the screening device for the donor intestinal flora, such as a plug-in hard disk, a smart memory card (Smart Media Card, SMC), a secure digital (Secure Digital, SD) card, a flash card (FlashCard), etc. equipped on the screening device for the donor intestinal flora. Further, the memory 720 may also include both an internal storage unit and an external storage device of the screening device for the donor intestinal flora. The memory 720 is used to store application software and various types of data installed in the screening device for the donor intestinal flora, such as program codes for installing the screening device for the donor intestinal flora. The memory 720 may also be used to temporarily store data that has been output or is to be output. In one embodiment, the memory 720 stores a screening program 740 for the donor intestinal flora, and the screening program 740 for the donor intestinal flora can be executed by the processor 710, thereby implementing the screening method for the donor intestinal flora of each embodiment of the present application.

[0129] In some embodiments, the processor 710 may be a central processing unit (CPU), a microprocessor or other data processing chip, used to run program codes or process data stored in the memory 720, such as executing a method for screening a donor's intestinal flora.

[0130] In some embodiments, the display 730 may be an LED display, a liquid crystal display, a touch-sensitive liquid crystal display, an OLED (Organic Light-Emitting Diode) touch device, etc. The display 730 is used to display information on the screening device for the donor intestinal flora and to display a visual user interface. The components 710-730 of the screening device for the donor intestinal flora communicate with each other via a system bus.

[0131] In one embodiment, when the processor 710 executes the donor intestinal flora screening program 740 in the memory 720, the steps in the above donor intestinal flora screening method are implemented.

[0132] This embodiment further provides a computer-readable storage medium on which a path planning program is stored. When the path planning program is executed by a processor, the following steps are implemented:

[0133] Based on preset rules, the initial donors are evaluated and qualified donors who meet the requirements are selected;

[0134] Process and analyze the fecal samples of qualified donors for a preset number of times to obtain initial intestinal flora data and build a donor database;

[0135] The subsequent intestinal flora data of qualified donors are used as a test set, and the weighted offset of the intestinal flora of the test set is calculated;

[0136] The weighted offset of the intestinal flora of the test set was compared with the donor database to screen the subsequent intestinal flora of qualified donors.

[0137] In summary, the present embodiment provides a method, device, equipment and storage medium for screening the intestinal flora of a donor, the method comprising: based on preset rules, evaluating the initial donor to screen out qualified donors that meet the requirements; processing and analyzing the fecal samples of the qualified donors for a preset number of times to obtain initial intestinal flora data and construct a donor database; using the subsequent intestinal flora data of the qualified donor as a test set, calculating the weighted offset of the intestinal flora of the test set; comparing the weighted offset of the intestinal flora of the test set with the donor database, and screening the subsequent intestinal flora of the qualified donor. The present invention first screens out qualified donors, then determines the initial intestinal flora data of the qualified donors based on the feces of the qualified donors and establishes a donor database based on this, then analyzes the subsequent feces of the qualified donors to extract the subsequent intestinal flora data, and compares the weighted offset with the donor database by calculating the weighted offset, thereby screening the subsequent intestinal flora of the qualified donors, and when the subsequent intestinal flora of the qualified donors changes, the unqualified intestinal flora can be promptly eliminated, thereby ensuring the uniformity of the subsequent intestinal flora of the qualified donors.

[0138] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any changes or substitutions that can be easily conceived by any technician familiar with the technical field within the technical scope disclosed by the present invention should be covered within the protection scope of the present invention.

Claims

1. A method for screening donor intestinal flora, characterized in that: include: Based on preset rules, the initial donors are evaluated and qualified donors who meet the requirements are selected; Process and analyze the fecal samples of qualified donors for a preset number of times to obtain initial intestinal flora data and build a donor database; The subsequent intestinal flora data of qualified donors are used as a test set, and the weighted offset of the intestinal flora of the test set is calculated; Comparing the weighted offset of the intestinal flora of the test set with the donor database, and screening subsequent intestinal flora of qualified donors; The intestinal flora data includes: the percentage content of each flora in the intestinal flora and the intestinal flora abundance value; the subsequent intestinal flora data of the qualified donor is used as a test set to calculate the weighted offset of the intestinal flora of the test set, including: Calculate the weight coefficient of the intestinal flora with a preset ranking according to the test set and the initial intestinal flora data; Calculating the flora offset of the test set according to the intestinal flora abundance value of the test set and the donor database; The weighted offset of the intestinal flora of the test set is determined according to the weight coefficient and the flora offset of the test set.

2. The method for screening donor intestinal flora according to claim 1, characterized in that: The method of processing and analyzing the fecal samples of the qualified donors for a preset number of times to obtain the initial intestinal flora data and construct the donor database includes: Extracting DNA from a preset number of stool samples of the qualified donor, and sequencing the DNA to obtain an original sequence; Preprocessing, species annotation and abundance analysis are performed on the original sequence to obtain intestinal flora markers and intestinal flora abundance values; Processing the intestinal flora marker to determine the percentage content of each flora in the intestinal flora; A donor database is established by the percentage content of each flora in the preset ranked intestinal flora and the abundance value of the intestinal flora.

3. The method for screening donor intestinal flora according to claim 1, characterized in that: The step of calculating the weight coefficient of the preset ranked intestinal flora according to the test set and the initial intestinal flora data includes: Arranging the intestinal flora abundance values ​​of the test set and the intestinal flora abundance values ​​of the donor database according to a first preset order to obtain the rank of the intestinal flora; The ranks of the same intestinal flora abundance values ​​are updated to the average of the ranks of the corresponding intestinal flora; The ranks of the preset ranked intestinal flora are sorted according to a second preset order to obtain a weight coefficient of the intestinal flora.

4. The method for screening donor intestinal flora according to claim 1, characterized in that: The step of determining the flora offset of the test set according to the intestinal flora abundance value of the test set and the donor database includes: When the intestinal flora abundance value of the test set is higher than the intestinal flora abundance value in the donor database, setting the flora offset of the test set according to a first offset rule; When the intestinal flora abundance value of the test set is lower than the intestinal flora abundance value in the donor database, the flora offset of the test set is set according to the second offset rule.

5. The method for screening donor intestinal flora according to claim 1, characterized in that: When the intestinal flora abundance value of the test set is higher than the intestinal flora abundance value in the donor database, setting the flora offset of the test set according to the first offset rule includes: When the intestinal flora abundance value of the test set is higher than the intestinal flora abundance value of the first proportion in the donor database, the flora offset is a first preset value; When the intestinal flora abundance value of the test set is higher than the intestinal flora abundance value of the second proportion in the donor database and lower than the intestinal flora abundance value of the first proportion in the donor database, the flora offset is a second preset value; When the intestinal flora abundance value of the test set is higher than the intestinal flora abundance value of the third proportion in the donor database and lower than the intestinal flora abundance value of the second proportion in the donor database, the flora offset is a third preset value; When the intestinal flora abundance value of the test set is higher than the intestinal flora abundance value of the fourth proportion in the donor database and lower than the intestinal flora abundance value of the third proportion in the donor database, the flora offset is a fourth preset value.

6. The method for screening donor intestinal flora according to claim 5, characterized in that: When the intestinal flora abundance value of the test set is lower than the intestinal flora abundance value in the donor database, setting the flora offset of the test set according to the second offset rule includes: When the intestinal flora abundance value of the test set is lower than the intestinal flora abundance value of the first proportion in the donor database, the flora offset is the first preset value; When the intestinal flora abundance value of the test set is lower than the intestinal flora abundance value of the second proportion in the donor database and higher than the intestinal flora abundance value of the first proportion in the donor database, the flora offset is the second preset value; When the intestinal flora abundance value of the test set is lower than the intestinal flora abundance value of the third proportion in the donor database and higher than the intestinal flora abundance value of the second proportion in the donor database, the flora offset is the third preset value; When the intestinal flora abundance value of the test set is lower than the intestinal flora abundance value of the fourth proportion in the donor database and higher than the intestinal flora abundance value of the third proportion in the donor database, the flora offset is the fourth preset value.

7. A screening device for donor intestinal flora, characterized in that: include: The donor screening module is used to evaluate the initial donors and select qualified donors that meet the requirements based on preset rules; A construction module is used to process and analyze a preset number of stool samples from qualified donors to obtain initial intestinal flora data and construct a donor database; A calculation module, used to use the subsequent intestinal flora data of the qualified donor as a test set and calculate the weighted offset of the intestinal flora of the test set; A flora screening module, used to compare the weighted offset of the intestinal flora of the test set with the donor database, and screen the subsequent intestinal flora of qualified donors; The intestinal flora data includes: the percentage content of each flora in the intestinal flora and the intestinal flora abundance value; the subsequent intestinal flora data of the qualified donor is used as a test set to calculate the weighted offset of the intestinal flora of the test set, including: Calculate the weight coefficient of the intestinal flora with a preset ranking according to the test set and the initial intestinal flora data; Calculating the flora offset of the test set according to the intestinal flora abundance value of the test set and the donor database; The weighted offset of the intestinal flora of the test set is determined according to the weight coefficient and the flora offset of the test set.

8. A screening device for donor intestinal flora, characterized in that: comprising a memory and a processor, wherein: The memory is used to store programs; The processor is coupled to the memory and is used to execute the program stored in the memory to implement the steps in the method for screening the donor intestinal flora as described in any one of claims 1 to 6.

9. A computer-readable storage medium, characterized in that: Used to store computer-readable programs or instructions, which, when executed by a processor, can implement the steps of the method for screening the donor intestinal flora as described in any one of claims 1 to 6.

Images