A mite-killing composition and its application

Abstract

This invention belongs to the field of acaricide technology and discloses an acaricide composition and its application. The acaricide composition comprises active ingredient A and active ingredient B. Active ingredient A is a compound of formula I, and active ingredient B is selected from either tetradifon or thiamethoxam. The mass ratio of active ingredient A to active ingredient B is 1:50 to 50:1. The acaricide composition of this invention can be used to prevent and control the spread and damage of common agricultural or forestry mites, with a significant synergistic effect and is safe for beneficial insects.

Description

Technical Field

[0001] This invention belongs to the field of pesticide acaricide technology, and discloses an acaricide composition and its application. Background Technology

[0002] Mites are arachnid pests and one of the most important pests of many crops in my country. These pests are tiny, reproduce rapidly, mutate quickly, and easily develop resistance to pesticides, making chemical control very difficult.

[0003] Combining chemical control with biological control can effectively control the damage caused by mites while reducing the dosage of pesticides. Therefore, it is particularly important to coordinate the relationship between biological and chemical control, reduce the impact of pesticides on the environment, natural enemies, and pest resistance, and seek chemical pesticides that are highly effective against target pests and have less impact on natural enemies.

[0004] Therefore, the applicant of this invention selects compound of formula I and any one of thiamethoxam or tetradifon for reasonable compounding to determine the optimal ratio that has a synergistic effect on the target mites and is safe for natural enemies, thereby reducing the dosage of the pesticide, protecting the natural enemies of the mites to the greatest extent, providing a basis for the rational use of acaricides, achieving safe use of pesticides, and reducing the impact of pesticides on the environment and natural enemies. Summary of the Invention

[0005] To address the problems in the existing technology, the present invention provides an acaricidal composition that exhibits a good synergistic effect against common plant mites and is essentially non-toxic to beneficial insects, reducing the dosage of pesticides used. While controlling mites, it maximizes the protection of natural enemies. Furthermore, the formulation made from this acaricidal composition has excellent rapid-acting properties and a long-lasting effect, reducing environmental pollution.

[0006] To achieve the above objectives, the present invention adopts the following technical solution: an acaricidal composition, wherein the acaricidal composition comprises active ingredient A and active ingredient B, wherein active ingredient A is a compound represented by formula I: The active ingredient B is selected from either thiamethoxam or tetradifon.

[0007] Furthermore, the mass ratio of active ingredient A to active ingredient B is 1:50 to 50:1;

[0008] Furthermore, the active ingredient B is thiamethoxam, and the mass ratio of active ingredient A to active ingredient B is 1:45 to 45:1.

[0009] The active ingredient B is tetradifon, and the mass ratio of active ingredient A to active ingredient B is 1:42 to 36:1.

[0010] Furthermore, the active ingredient B is thiamethoxam, and the mass ratio of active ingredient A to active ingredient B is 1:22 to 35:1.

[0011] The active ingredient B is tetradifon, and the mass ratio of active ingredient A to active ingredient B is 1:20 to 30:1;

[0012] Furthermore, the acaricide composition also includes auxiliary components, which are carriers or adjuvants;

[0013] Further, the wetting agent is selected from one or more of alkylbenzene sulfonates, alkylnaphthalene sulfonates, lignin sulfonates, sodium dodecyl sulfate, sodium dioctyl succinate sulfonate, α-olefin sulfonates, alkylphenol polyoxyethylene ethers, castor oil polyoxyethylene ethers, alkylphenol ethoxylates, fatty alcohol ethoxylates, sodium fatty alcohol polyoxyethylene ether sulfate, silkworm excrement, soapberry powder, soapberry powder, SOPA, detergents, emulsifiers 2000 series, and wetting and penetrating agents F; and / or

[0014] The dispersant is selected from one or more of the following: lignin sulfonate, alkylnaphthalene sulfonate formaldehyde condensate, naphthalene sulfonate, tristyrylphenol ethoxylate phosphate, fatty alcohol ethoxylate, alkylphenol polyoxyethylene ether, alkylphenol polyoxyethylene ether formaldehyde condensate sulfate, fatty amine polyoxyethylene ether, glycerol fatty acid ester polyoxyethylene ether, polycarboxylates, polyacrylic acids, phosphates, EO-PO block copolymers, and EO-PO graft copolymers; and / or

[0015] The thickener is selected from one or more of xanthan gum, polyvinyl alcohol, organobentonite, magnesium aluminum silicate, and carboxymethyl cellulose; and / or

[0016] The disintegrant is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid, and tartaric acid; and / or

[0017] The emulsifier is selected from one or more of the following: fatty alcohol polyoxyethylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, phenethylphenol polyoxyethylene polyoxypropylene ether, alkylphenol polyoxyethylene ether, fatty amine polyoxyethylene ether, alkylbenzene sulfonate, styrene-phenol polyoxyethylene ether, and fatty acid polyoxyethylene ester; and / or

[0018] The defoamer is selected from silicone oil, C 10 ~C 20 Saturated fatty acid compounds, C8-C 10 One or more of fatty alcohols or silicone compounds; and / or

[0019] The preservative is selected from one or more of sorbic acid, sodium sorbate, potassium sorbate, benzoic acid, sodium benzoate, sodium p-hydroxybenzoate, and methyl p-hydroxybenzoate; and / or

[0020] The stabilizer is selected from one or more of disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-cresol, and epoxidized vegetable oil; and / or

[0021] The synergist is selected from synergistic phosphorus, synergistic ether; and / or

[0022] The carrier is selected from one or more of kaolin, bentonite, attapulgite, light calcium carbonate, diatomaceous earth, and precipitated silica; and / or

[0023] The solvent is selected from one or more of benzene, toluene, xylene, methanol, ethanol, isopropanol, n-butanol, diesel oil, N,N-dimethylformamide, cyclohexanone, ethyl acetate, N-methylpyrrolidone, propanol, butanol, ethylene glycol, diethylene glycol, ethylene glycol methyl ether, butyl ether, solvent oil, vegetable oil, vegetable oil derivatives, and deionized water; and / or

[0024] All of the above auxiliary ingredients are commercially available.

[0025] Furthermore, the total weight of active ingredient A and active ingredient B accounts for 0.5% to 80% of the total weight of the acaricide composition;

[0026] Furthermore, the acaricidal composition can be prepared into a liquid formulation or a solid formulation. The liquid formulation is selected from soluble agents, soluble gels, oils, spreading oils, emulsifiable concentrates, latexes, dispersible liquids, ointments, water emulsions, oil emulsions, microemulsions, lipid suspensions, microcapsule suspensions, oil suspensions, dispersible oil suspensions, suspension emulsions, microcapsule suspension-suspension, microcapsule suspension-water emulsions, or microcapsule suspension-suspension emulsions. The solid formulation is selected from powders, granules, balls, tablets, strips, wettable powders, oil-dispersible powders, emulsion powders, water-dispersible granules, emulsion granules, water-dispersible tablets, soluble powders, soluble tablets, or soluble granules.

[0027] Furthermore, the formulation is a suspension, emulsifiable concentrate, microemulsion, water emulsion, water-dispersible granules, or wettable powder.

[0028] The present invention also discloses the use of the acaricidal composition described above for the prevention and control of plant mites.

[0029] Furthermore, the plants mentioned are agricultural crops or forestry crops;

[0030] Furthermore, the plant mites mentioned are mites belonging to the families Tetranychidae, Trichophytoncidae, Eriophyta, and / or Ephedraidae.

[0031] Furthermore, the spider mite pests are two-spotted spider mite, carmine spider mite, truncated spider mite, apple spider mite, citrus spider mite and / or wheat spider mite; the gall mite pests are citrus rust mite, wheat gall mite and / or wolfberry gall mite; and the true spider mite pests are wheat red spider mite.

[0032] The beneficial effects of the present invention are as follows: the acaricide composition of the present invention rationally combines acaricides with different mechanisms of action, exhibits a good synergistic effect on common harmful mites, and is safe for natural enemies; it reduces the dosage of the agent, has good rapid effect, and a long-lasting effect. Detailed Implementation

[0033] To make the technical solution, objectives and advantages of the present invention clearer, the present invention is described with reference to the following specific embodiments. However, the present invention can be implemented in various forms and should not be limited to the embodiments described herein.

[0034] Formulation preparation example:

[0035] Preparation Example 1: 14% Formula I compound·thiamethoxam aqueous emulsion (1:6)

[0036] Formula: 2% Formula I compound, 12% thiamethoxam, 1% Gelbert alcohol polyoxyethylene ether, 6% alkyl aryl polyoxyethylene ether polyoxypropylene ether, 8% cyclohexanone, 0.2% xanthan gum, 5% ethylene glycol, 0.1% sodium benzoate, 0.1% silicone oil, deionized water to make up the balance;

[0037] Preparation method: According to the formulation ratio in the preparation example, the active ingredient is dissolved in the solvent and an emulsifier is added to form a homogeneous oil phase. Deionized water and antifreeze are mixed together to form a homogeneous aqueous phase. Under high-speed shearing, the aqueous phase is added to the oil phase to form a well-dispersed water emulsion product.

[0038] Preparation Example 2: 5% Formula I compound·thiamethoxam microemulsion (4:1)

[0039] Formula: 4% Formula I compound, 1% thiamethoxam, 1% alkylphenol polyoxyethylene ether phosphate, 10% styrene-phenol polyoxyethylene ether, 2% fatty alcohol polyoxyethylene ether sodium sulfate, 2% calcium dodecylbenzenesulfonate, 5% alkyl glycoside, 2% xylene, 7% cyclohexanone, 3% ethylene glycol, 0.1% Kathon, 0.5% glycerin, 0.1% xanthan gum, deionized water to make up the balance;

[0040] Preparation method: According to the formulation ratio of the preparation example, the active ingredients, solvent, emulsifier, etc. are mixed evenly to obtain the oil phase. The antifreeze and water are mixed evenly to obtain the aqueous phase. The oil phase is added to the aqueous phase under stirring and stirred evenly. Shearing is continued for 10 minutes. Then, silicone oil, defoamer, xanthan gum, etc. are added and stirred evenly to obtain small droplets with oil phase particles of 0.01-0.1 micrometers, which is the microemulsion of the present invention.

[0041] Preparation Example 3: 9% Formula I compound·thiamethoxam emulsifiable concentrate (8:1)

[0042] Formula: 8% Formula I compound, 1% thiamethoxam, 10% DMF, 8% alkylphenol polyoxyethylene ether, 2% calcium dodecylbenzenesulfonate, 15% propylene carbonate, xylene to make up the balance;

[0043] Preparation method: The measured active ingredients, solvent, and co-solvent are added to a mixing tank and stirred to dissolve them. Then, the emulsifier is added, and the remaining solvent is used to make up the balance. The mixture is stirred evenly in a mixing tank and filtered to obtain the emulsifiable oil required by this invention.

[0044] Preparation Example 4: 22% Formula I compound·thiamethoxam wettable powder (10:1)

[0045] Formula: 20% Formula I compound, 2% thiamethoxam, 3% sodium polycarboxylate, 8% naphthalene sulfonate formaldehyde condensate, 2% stretching powder BX, 5% silica, and kaolin to make up the balance;

[0046] Preparation method: The active ingredients, dispersant, wetting agent and filler are mixed according to the formula ratio, stirred evenly in a stirring tank, and then pulverized and mixed evenly multiple times by an air jet mill to prepare the wettable powder of the composition of the present invention.

[0047] Preparation Example 5: 18% Formula I compound·thiamethoxam suspension (5:1)

[0048] Formula: 15% Formula I compound, 3% thiamethoxam, 2% isotridecyl alcohol polyoxyethylene ether, 4% styrene phenol polyoxyethylene ether phosphate, 1% sodium lignosulfonate, 0.25% xanthan gum, 3% ethylene glycol, 0.5% Kathon, 0.5% silicone oil, deionized water to make up the balance;

[0049] Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in the reaction vessel in sequence, water is added and mixed evenly, and then subjected to high-speed shearing, wet sand milling and finally homogenization filtration to obtain the suspension product.

[0050] Preparation Example 6: 18% Formula I compound·tetradamine suspension (1:8)

[0051] Formula: 2% Formula I compound, 16% tetradifon, 1% alkyl aryl polyoxyethylene ether polyoxypropylene ether, 1% sodium lignosulfonate, 3% styrene phenol polyoxyethylene ether sulfate, 2% fatty alcohol polyoxyethylene ether, 0.5% magnesium aluminum silicate, 0.2% xanthan gum, 5% ethylene glycol, 0.2% potassium benzoate, 0.5% silicone oil, deionized water to make up the balance;

[0052] Preparation method: Same as in preparation example 5.

[0053] Preparation Example 7: 18% Formula I compound·tetradacin wettable powder (8:1)

[0054] Formula: 16% Formula I compound, 2% tetradifon, 4% sodium polycarboxylate, 8% sodium lignosulfonate, 2% bleaching powder BX, 5% silica, kaolin to make up the balance;

[0055] Preparation method: Same as in preparation example 4.

[0056] Preparation Example 8: 33% Formula I compound tetradifon water-dispersible granules (10:1)

[0057] Formula: 30% Formula I compound, 3% tetradifon, 1% succinate sulfonate, 10% naphthalene sulfonate formaldehyde condensate, 5% sodium polycarboxylate, 1% sodium dodecyl sulfate, 5% silica, 30% starch, and kaolin to make up the balance.

[0058] Preparation method: According to the formulation ratio in the example, add the active ingredient to the carrier, and add surfactants and other functional additives therein, mix, and after air jet pulverization, add 10-25% water, and then knead, granulate, dry and sieve to obtain water-dispersible granules; or spray water, granulate and dry the pulverized powder in a fluidized bed granulator, and then sieve to obtain the product.

[0059] Indoor activity test

[0060] Example 1: Indoor bioactivity test of compound I combined with tetradifon or thiamethoxam on two-spotted spider mites.

[0061] The indoor test was conducted in accordance with NY / T 1154.12-2008 "Guidelines for Indoor Bioassay Tests of Pesticides - Insecticides - Part 12: Tetranychus Slide Immersion Method".

[0062] Biological test material: Two-spotted spider mite nymphs.

[0063] Test material preparation: Select nymphs that are kept indoors and have the same physiological condition. Cut double-sided tape into 2cm lengths and stick them to one end of a glass slide. Then select healthy mites and stick their backs onto the double-sided tape. Place 30 mites per slide and put them in a rearing box lined with a damp sponge. Cover the box and place it at (25±1)℃. After 2 hours, examine the slide under a microscope, remove dead and injured individuals, and replenish the slide to 30 mites.

[0064] Preparation of reagents: Dissolve the experimental compound I technical, tetradifon technical, and thiamethoxam technical in acetone and prepare a stock solution. Then, prepare five series of mass concentrations using 0.1% Tween-80 aqueous solution in equal proportions.

[0065] Chemical treatment: Immerse the glass slide in the chemical solution and gently shake for 5 seconds. Remove the slide, absorb the excess solution with absorbent paper, place it on a white porcelain dish lined with a damp sponge, and cover it with a transparent plastic film.

[0066] Each treatment was repeated four times, with a blank control consisting of a treatment without any reagents (containing all organic solvents and emulsifiers).

[0067] Feeding and observation: Place the container containing the treated test insects under suitable conditions for feeding and observation.

[0068] Inspection: Check the mortality of test insects 48 hours after treatment, and record the total number of insects and the number of dead insects.

[0069] Data statistics and analysis:

[0070] Based on the survey data, the corrected mortality rates for each treatment were calculated using the following formula, and the results were rounded to two decimal places.

[0071]

[0072] In the formula:

[0073] P – Mortality rate, expressed as a percentage (%);

[0074] K represents the number of dead insects, in heads;

[0075] N represents the total number of insects treated, in units of heads.

[0076]

[0077] In the formula:

[0078] P1 – Corrected mortality rate, in percentage (%);

[0079] P t —The mortality rate is expressed as a percentage (%).

[0080] P0 – Mortality rate in the blank control group, expressed as a percentage (%).

[0081] If the control mortality rate is <5%, no correction is needed; if the control mortality rate is between 5% and 20%, correction should be performed according to the corrected mortality rate formula; if the control mortality rate is >20%, the trial needs to be repeated.

[0082] The LC was determined using the DPS statistical analysis system. 50 The value is used to evaluate the activity of the test reagent on the biological sample.

[0083] The co-toxicity coefficient (CTC value) of the mixture is calculated using the following formula:

[0084]

[0085] In the formula:

[0086] ATI – Actual Measured Toxicity Index of Mixtures;

[0087] S – LC50 of standard acaricides 50 The unit is milligrams per liter (mg / L);

[0088] M – LC of the mixture 50 The unit is milligrams per liter (mg / L).

[0089] TTI = TI A ×P A +TI B ×P B

[0090] In the formula:

[0091] TTI – Theoretical Toxicity Index of Mixtures;

[0092] TI A —A. Toxicity index of drug A;

[0093] P A —Percentage content of drug A in the mixture, expressed as percentage (%);

[0094] TI B —Toxicity index of drug B;

[0095] P B —Percentage content of agent B in the mixture, expressed as percentage (%).

[0096]

[0097] In the formula:

[0098] CTC – Cotoxicity Coefficient;

[0099] ATI – Actual Measured Toxicity Index of Mixtures;

[0100] TTI – Theoretical Toxicity Index of Mixtures.

[0101] The co-toxicity coefficients (CTC) of the compound formulations were ≥120, indicating a synergistic effect; CTC ≤80, indicating an antagonistic effect; and 80 < CTC < 120, indicating an additive effect. The results of the indoor tests are shown in the table below:

[0102] Table 1. Indoor bioactivity tests of compound I and thiamethoxam on nymphs of Tetranychus bisa.

[0103] Test reagents <![CDATA[LC 50 (mg / L)]]> (ATI) (TTI) Cotoxicity coefficient (CTC) Compound (A) of Formula I 2.5833 100.000 / / <![CDATA[Hexythiazox (B1)]]> 11.4095 22.642 / / <![CDATA[A:B1=1:45]]> 10.0802 25.627 24.323 105.362 <![CDATA[A:B1=1:22]]> 8.0102 32.250 26.005 124.015 <![CDATA[A:B1=1:15]]> 6.9503 37.168 27.477 135.272 <![CDATA[A:B1=1:8]]> 5.1082 50.572 31.237 161.896 <![CDATA[A:B1=4:1]]> 1.4992 172.312 84.528 203.851 <![CDATA[A:B1=8:1]]> 1.4924 173.097 91.405 189.374 <![CDATA[A:B1=12:1]]> 1.6093 160.523 94.049 170.680 <![CDATA[A:B1=20:1]]> 1.8227 141.729 96.316 147.150 <![CDATA[A:B1=25:1]]> 1.9812 130.391 97.025 134.389 <![CDATA[A:B1=35:1]]> 2.1049 122.728 97.851 125.423

[0104] Table 2 shows the indoor bioactivity test of compound I and tetradifon in combination with Tetradifon nymphs of Tetranychus spp. 2.

[0105] Test reagents <![CDATA[LC 50 (mg / L)]]> (ATI) (TTI) Cotoxicity coefficient (CTC) Compound (A) of Formula I 2.5833 100.000 / / <![CDATA[Clofentezine (B2)]]> 6.0274 42.859 / / <![CDATA[A:B2=1:42]]> 4.6004 56.154 44.188 127.079 <![CDATA[A:B2=1:21]]> 3.9406 65.556 45.457 144.217 <![CDATA[A:B2=1:11]]> 3.4753 74.333 47.621 156.093 <![CDATA[A:B2=1:5]]> 2.4377 105.973 52.383 202.305 <![CDATA[A:B2=4:3]]> 1.5097 171.113 75.511 226.607 <![CDATA[A:B2=7:1]]> 1.5554 166.086 92.857 178.861 <![CDATA[A:B2=10:1]]> 1.6932 152.569 94.805 160.929 <![CDATA[A:B2=15:1]]> 1.8856 137.001 96.429 142.075 <![CDATA[A:B2=22:1]]> 2.0573 125.567 97.516 128.767 <![CDATA[A:B2=36:1]]> 2.1081 122.542 98.456 124.464

[0106] Tables 1 and 2 show that each of the single agents of Formula I, tetradifon, and thiamethoxam exhibits high toxicity against *Tetranychus spp.*, with Formula I showing the best control efficacy. Formula I and thiamethoxam did not show antagonistic effects against *Tetranychus spp.* in the ratio range of 1:45–35:1. However, in the range of 1:22–25:1, their co-toxicity coefficient was greater than 120, indicating a synergistic effect. Similarly, Formula I and tetradifon showed a co-toxicity coefficient greater than 120 against *Tetranychus spp.* in the range of 1:42–36:1, also indicating a synergistic effect.

[0107] Example 2: Safety test of compound I combined with tetradifon or thiamethoxam in the natural enemy *Heterophyllum oxypetalum*

[0108] Experimental target: 3rd instar larvae of the ladybug.

[0109] Test reagents: The original drug was prepared into a stock solution using an organic solvent, and then dissolved in a 0.1% Tween-80 aqueous solution. The reagents were prepared according to the optimal ratio of compound I in Example 1 with tetradifon or thiamethoxam, and a blank control was set up with no reagents (containing all organic solvents and emulsifiers).

[0110] Test material preparation: Select clean cotton leaves that have grown uniformly and have not been treated with pesticides, cut them into leaf discs with a diameter of 6 cm, and lay them flat in plastic cups containing agar. Inoculate 200 two-spotted spider mites raised indoors onto the leaf discs, and inoculate one ladybug larvae in each cup after being starved for 4 hours.

[0111] Chemical treatment: Place the plastic cup in the potter spray tower for spraying. After the solution settles for 1 minute, remove the cup and seal the top with a mesh screen with a 0.15mm aperture to prevent the test insects from escaping.

[0112] Feeding and observation: The treated test insects were fed and observed under the conditions of temperature (25±1)℃, relative humidity (70±5)%, and photoperiod L:D=(16:8)h. After 48h, the mortality of the ladybugs was checked and the number of deaths was recorded. Those insects that did not move when touched with a brush were considered dead.

[0113] Each drug concentration and control were replicated four times, with each replicate testing 30 third-instar ladybug larvae.

[0114] Data statistics and analysis:

[0115] Based on the survey data, the corrected mortality rates for each treatment were calculated using the following formula, and the results were rounded to two decimal places.

[0116]

[0117] In the formula:

[0118] P – Mortality rate, expressed as a percentage (%);

[0119] K represents the number of dead insects, in heads;

[0120] N represents the total number of insects treated, in units of heads.

[0121]

[0122] In the formula:

[0123] P1 – Corrected mortality rate, in percentage (%);

[0124] P t —The mortality rate is expressed as a percentage (%).

[0125] P0 – Mortality rate in the blank control group, expressed as a percentage (%).

[0126] If the control mortality rate is <5%, no correction is needed; if the control mortality rate is between 5% and 20%, correction should be performed according to the corrected mortality rate formula; if the control mortality rate is >20%, the trial needs to be repeated.

[0127] The DPS statistical analysis system was used to analyze the data and derive the toxicity regression equation and LC. 50 The value is used to evaluate the activity of the test reagent on the biological sample.

[0128] The safety factor was used to evaluate the safety of various pesticides for ladybugs:

[0129]

[0130] When the toxicity selection index STR < 1, it indicates that the agent has a negative selectivity for natural enemies and pests (mites); when STR = 1, it means that the agent has no obvious selectivity for natural enemies and pests (mites); when 1 < STR ≤ 10, it indicates that the agent has a positive selectivity for natural enemies and pests (mites); when 10 < STR ≤ 100, it means that the agent has a moderate positive selectivity for natural enemies and pests; when 100 < STR ≤ 1000, it indicates that the agent has a high positive selectivity for natural enemies and pests (mites); when STR > 1000, it means that the agent has a strong positive selectivity for natural enemies and pests (mites).

[0131] Table 3 Results of the safety test of the compound of formula I in combination with clofentezine or hexythiazox against the natural enemy Hippodamia variegata

[0132]

[0133] As can be seen from Table 3, the toxicity of the compound of formula I in combination with clofentezine or hexythiazox against Hippodamia variegata is significantly lower than its toxicity against pest mites, and it is relatively safe for natural enemy insects.

[0134] Field efficacy test

[0135] Example 3: Field efficacy test of the acaricidal composition against Tetranychus cinnabarinus on cotton

[0136] In the cotton field of Yuanzhuang Village, Thirty Li Puzhen Town, Gaotang County, Liaocheng City, Shandong Province, the soil fertility is medium, and the cultivation conditions of all test plots are uniform and consistent with the local agricultural cultivation measures.

[0137] Test target: Tetranychus cinnabarinus.

[0138] Test method: The medicine was applied once at the initial stage of the occurrence of Tetranychus cinnabarinus. During the application, a knapsack sprayer was used for quantitative spraying, and no other pesticides for controlling pests and diseases were used during the test period.

[0139] Test investigation: Investigations were carried out 3 days, 7 days, and 14 days after the application. A total of 3 investigations were conducted. 20 leaves were fixed in each plot during the investigation, the number of active mites was counted, and the mite population reduction rate and control effect were calculated.

[0140] Safety investigation: At the same time of each test investigation, observe whether the pesticides in each treatment have abnormal effects on cotton growth and whether there are adverse effects on non-target organisms.

[0141] Test data statistics:

[0142]

[0143]

[0144] The test results are shown in the following table:

[0145] Table 4. Field efficacy test results of the acaricide composition against cotton red spider mites.

[0146]

[0147] Field efficacy trials showed that the acaricidal composition of this invention can effectively control cotton red spider mite damage, and each compound formulation has good rapid-acting properties and a long-lasting effect. Throughout the entire trial, no abnormal effects of the treatments on cotton growth or adverse effects on non-target organisms were observed.

[0148] Example 4: Field efficacy test of the acaricide composition against apple spider mites

[0149] This experiment was conducted in an apple orchard in Xiaoliu Village, south of Zhengzhou City, Henan Province. The apple variety was Tengmu No. 1, 15 years old, with a spacing of 2m × 4m and a tree height of about 2.5m. Apple trees had been severely infested with spider mites for several years. After field investigation and indoor microscopic examination, the main mite species was identified as hawthorn spider mite. The pesticide was applied on May 22, 2020 (sunny weather, temperature 21℃ / 35℃).

[0150] The test and control reagents are shown in the table below (Table 5).

[0151] Application method: Conventional application was used in the experiment. A backpack electric sprayer was used to apply the pesticide evenly to the entire fruit tree, ensuring that both the upper and lower surfaces of the leaves were sprayed.

[0152] Experimental design: The experimental plot area was 2 fruit trees, and each treatment was repeated 4 times.

[0153] Survey Methods: The experimental survey was conducted according to GB / T 17980.7-2000. The initial mite population was surveyed before pesticide application, and the number of active mites was surveyed at 3, 7, and 14 days after application. During the survey, for each tree in each plot, the number of active mites on 5 leaves were randomly collected from each of the 5 directions (east, south, west, north, and center). A handheld magnifying glass was used to examine the leaves, and the number of active mites was recorded.

[0154] Experimental data statistics:

[0155]

[0156]

[0157] The test results are shown in the table below:

[0158] Table 5. Results of field efficacy trials of the acaricidal composition against apple spider mites.

[0159]

[0160] Indoor toxicity tests and field efficacy tests show that the acaricidal composition of the present invention exhibits good control effect on harmful mites and is safe for natural enemy insects.

[0161] Although the present invention has been described in detail above with general description and specific embodiments, some modifications or improvements can be made to it, which will be obvious to those skilled in the art. Therefore, all such modifications or improvements made without departing from the spirit of the present invention are within the scope of protection claimed by the present invention.

Claims

1. A mite-killing composition, characterized in that, The acaricide composition comprises active ingredient A and active ingredient B, wherein active ingredient A is a compound of formula I: (Formula I), wherein the active ingredient B is thiamethoxam, and the mass ratio of active ingredient A to active ingredient B is 1:22~35:

1.

2. The acaricide composition according to claim 1, characterized in that, The acaricide composition further includes auxiliary components, which are carriers or adjuvants.

3. The acaricide composition according to claim 1, characterized in that, The total weight of active ingredient A and active ingredient B accounts for 0.5% to 80% of the total weight of the acaricide composition.

4. The acaricide composition according to claim 1, characterized in that, The acaricidal composition can be prepared as a liquid formulation or a solid formulation. The liquid formulation is selected from suspensions, soluble concentrates, emulsifiable concentrates, dispersible liquids, ointments, water emulsions, microemulsions, microcapsule suspensions, dispersible oil suspensions, or suspension emulsions. The solid formulation is selected from wettable powders, milk powders, water-dispersible granules, emulsion granules, water-dispersible tablets, soluble powders, soluble tablets, or soluble granules.

5. The acaricide composition according to claim 4, characterized in that, The formulation is a suspension, emulsifiable concentrate, microemulsion, water emulsion, dispersible oil suspension, water-dispersible granules, or wettable powder.

6. Use of the acaricidal composition according to any one of claims 1-5 for the prevention and control of two-spotted spider mites on plants.

7. The use according to claim 6, characterized in that, The plants mentioned are apples and cotton.

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