A laser speckle contrast blood flow imaging system and method with both transmissive and reflective capabilities

Abstract

This invention discloses a laser speckle contrast imaging system and method with shared transmission and reflection capabilities, relating to the field of optical imaging. In this system, coherent light is split into two beams by a beam splitter. Based on optical geometry principles, coherent transmitted and reflected light can coexist simultaneously, achieving shared transmission and reflection laser speckle contrast imaging. Furthermore, by utilizing the number of optical paths present in the first, second, and third light-shielding plates, either transmission-type or reflection-type laser speckle contrast imaging can be achieved. This invention can improve the imaging depth of blood vessels while maintaining the imaging quality of superficial blood vessels.

Description

Technical Field

[0001] This invention relates to the field of optical imaging, and in particular to a laser speckle contrast blood flow imaging system and method that uses both transmission and reflection capabilities. Background Technology

[0002] Laser speckle contrast imaging (LSI) provides real-time, wide-field-of-view, and high spatiotemporal resolution blood flow images without the need for contrast agents or scanning. Currently, in basic research, such as acquiring raw speckle image sequences of the mouse brain, the thickness of the brain prevents transmitted light from penetrating, thus requiring the use of reflective laser speckle contrast imaging systems. However, in acquiring raw speckle image sequences of the mouse ear or paw, transmitted light can pass through the corresponding areas, and LSI has advantages over reflective laser speckle contrast imaging in detecting deep blood flow, making it a viable option. However, in practical applications, LSI and reflective laser speckle contrast imaging systems are separate systems, making switching between them inconvenient.

[0003] Most laser speckle contrast imaging systems are reflective systems with shallow imaging depth. Recent studies have shown that transmission laser speckle contrast imaging has advantages over reflection laser speckle contrast imaging in deep blood flow imaging; however, reflection laser speckle contrast imaging is superior to transmission laser speckle contrast imaging in shallow blood flow imaging.

[0004] Therefore, there is an urgent need to provide a laser speckle contrast imaging system that can achieve both transmission and reflection laser speckle contrast imaging. Summary of the Invention

[0005] The purpose of this invention is to provide a laser speckle contrast imaging system and method that can achieve both transmission and reflection laser speckle contrast imaging, thereby improving the imaging depth of blood vessels while ensuring the imaging quality of superficial blood vessels.

[0006] To achieve the above objectives, the present invention provides the following solution:

[0007] A laser speckle contrast blood flow imaging system with both transmission and reflection capabilities includes: a laser source module, an optical path module, a stage, and an image acquisition module;

[0008] The laser source module is used to generate coherent light with a set laser power to the optical path module;

[0009] The platform is used to place the object to be tested.

[0010] The optical path module includes: a beam splitter, a convex lens, a first reflecting mirror, a second reflecting mirror, a third reflecting mirror, a first plano-concave cylindrical lens, a second plano-concave cylindrical lens, a first light-shielding plate, a second light-shielding plate, and a third light-shielding plate. When none of the first, second, and third light-shielding plates are placed, the coherent light generated by the laser source module is split by the beam splitter into an upper laser beam and a lower laser beam. The upper laser beam is converged by the convex lens and then illuminates the first reflecting mirror. After being expanded by the first plano-concave cylindrical lens, the upper laser beam is then illuminated by the third reflecting mirror and then illuminated by the object under test. The lower laser beam is expanded by the second plano-concave cylindrical lens and then illuminated by the second reflecting mirror and then illuminated by the object under test. The image acquisition module then collects the two coherent transmitted and reflected light simultaneously generated on the object, obtaining a shared original speckle image sequence, thus achieving shared laser speckle contrast imaging. When the second light-shielding plate is placed, or when the first and third light-shielding plates are placed simultaneously, there is no coherent transmitted or reflected light. When only the first light-shielding plate is placed, a transmitted original speckle image sequence is acquired based on the generated coherent transmitted light, achieving transmitted laser speckle contrast imaging. When only the third light-shielding plate is placed, a reflected original speckle image sequence is acquired based on the generated coherent reflected light, achieving reflected laser speckle contrast imaging. The convex lens is located between the beam splitter and the first reflecting mirror; the stage is located between the second reflecting mirror and the image acquisition module.

[0011] The angle between the first reflector and the horizontal line is the same as the angle between the second reflector and the horizontal line; the center of the coherent light emitted by the laser source module is on the same horizontal line as the geometric center of the beam splitter, the second plano-concave cylindrical lens, and the second reflector; the geometric centers of the first reflector, the first plano-concave cylindrical lens, and the third reflector are on the same horizontal line; the geometric centers of the beam splitter, the convex lens, and the first reflector are on the same vertical line.

[0012] The image acquisition module is used to acquire transmission-type raw speckle image sequences, reflection-type raw speckle image sequences, and transmission-reflection combined raw speckle image sequences.

[0013] Optionally, the laser source module includes: a laser controller and a laser;

[0014] The laser controller and the laser are connected via a current drive line and a temperature control line;

[0015] The laser, under the control of the laser controller, is used to generate coherent light with a set laser power.

[0016] Optionally, the image acquisition module includes: a camera, an adjustable microscope tube, and a microscope objective;

[0017] The adjustable lens barrel is used to adjust the distance between the camera and the microscope objective by rotating it;

[0018] The geometric centers of the second reflecting mirror, the stage, and the microscope objective are located on the same vertical line.

[0019] Optionally, half the length of the third reflecting mirror is Half the length of the camera is The horizontal distance between the third reflecting mirror and the geometric center of the camera is greater than .

[0020] Optionally, the shelf has a light-transmitting hole at its geometric center.

[0021] Optionally, the third reflector is used to rotate freely around a horizontal central axis, thereby forming different angles with the horizontal line.

[0022] A laser speckle contrast imaging method using both transmission and reflection capabilities, applied to the aforementioned laser speckle contrast imaging system, includes:

[0023] Adjust the laser controller to make the laser generate coherent light with a set laser power to the optical path module;

[0024] When the first, second, and third light-shielding plates are not placed, the camera is used to collect the two coherent transmitted and reflected light simultaneously to obtain the original speckle image sequence with shared transmission and reflection.

[0025] The spatial speckle contrast image is calculated based on the original speckle image sequence with shared perspective and reflection.

[0026] Optionally, the step of calculating the spatial speckle contrast image based on the original speckle image sequence shared by the perspective and reflection methods specifically includes:

[0027] Using formula Determine spatial speckle contrast image ;

[0028] in, The size of the sliding space window, Let z be the pixel coordinates, and z ranges from [1, Z], where Z is the original speckle image sequence. Frame count, The value range is [1, row- +1], row is the original speckle image sequence the number of rows, The range of values ​​for is [1, col- +1], col is the original speckle image sequence The number of columns.

[0029] According to specific embodiments provided by the present invention, the present invention discloses the following technical effects:

[0030] This invention provides a laser speckle contrast imaging system and method with shared transmission and reflection capabilities. It utilizes a beam splitter to divide the laser beam into two paths, allowing coherent transmitted and reflected light to coexist in the system, thus achieving shared transmission and reflection laser speckle contrast imaging. Simultaneously, a light-shielding plate controls the number of optical paths in the system, enabling either transmission-type or reflection-type laser speckle contrast imaging. By simultaneously illuminating the object under test with coherent transmitted and reflected light, a sequence of original speckle images is acquired. This sequence is then processed using a spatial speckle contrast imaging method, resulting in shared transmission and reflection laser speckle contrast images that improve the imaging depth of blood vessels while maintaining the imaging quality of superficial blood vessels. Attached Figure Description

[0031] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0032] Figure 1 A schematic diagram of a laser speckle contrast imaging system for both transmission and reflection provided by the present invention;

[0033] Figure 2 A front view of a laser speckle contrast blood flow imaging system with both transflection and reflection capabilities provided by the present invention in one embodiment;

[0034] Figure 3 A side view of a laser speckle contrast blood flow imaging system with both transflection and reflection capabilities provided by the present invention in one embodiment;

[0035] Figure 4 A schematic diagram of a phantom cross-section prepared in one embodiment of the laser speckle contrast imaging method for transmissive and reflective imaging provided by the present invention;

[0036] Figure 5 A comparative image of a transmission-reflection laser speckle contrast image, a reflection laser speckle contrast image, and a transmission-reflection laser speckle contrast image obtained from a phantom experiment in one embodiment of the laser speckle contrast method for blood flow imaging with shared transmission and reflection capabilities provided by the present invention. Attached Figure Description

[0038] 1. Laser controller; 2. Current drive line; 3. Temperature control line; 4. Laser; 4-0. Laser mount; 5. Beam splitter; 5-0. Beam splitter mount; 6. Convex lens; 6-0. Convex lens mount; 7-1. First reflector; 7-1-0. First reflector mount; 7-2. Second reflector; 7-2-0. Second reflector mount; 8-1. First plano-concave cylindrical lens; 8-1-0. First plano-concave cylindrical lens mount; 8-2. Second plano-concave cylindrical lens mount. Lens; 8-2-0, Second plano-concave cylindrical lens mounting base; 9, Third reflecting mirror; 9-0, Third reflecting mirror mounting base; 10, Stage; 10-1, Adjustable light-transmitting aperture light shield; 11, Camera; 12, Adjustable lens barrel; 13, Microscope objective; 14-1, First light shield; 14-2-, Second light shield; 14-3, Third light shield; 15, Vertical moving stage; 16, Fixed bracket; 17, First system bracket; 18, Second system bracket; 19, Connecting sleeve. Detailed Implementation

[0039] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0040] The purpose of this invention is to provide a laser speckle contrast imaging system and method that combines transmission and reflection, enabling both transmission and reflection laser speckle contrast imaging. Furthermore, by employing a laser speckle contrast imaging method that combines transmission and reflection, the imaging depth of blood vessels can be improved while ensuring the imaging quality of superficial blood vessels.

[0041] To make the above-mentioned objects, features and advantages of the present invention more apparent and understandable, the present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0042] like Figure 1 As shown, the laser speckle contrast blood flow imaging system with both transmission and reflection capabilities provided by the present invention includes: a laser source module, an optical path module, a stage, and an image acquisition module.

[0043] The laser source module includes a laser controller 1 and a laser 4, wherein the laser controller 1 and the laser 4 are connected through a current drive line 2 and a temperature control line 3 to generate stable coherent light with controllable laser power.

[0044] As a specific embodiment, the coherent light wavelength is The laser power is .

[0045] The optical path module includes a beam splitter 5, a convex lens 6, a first reflecting mirror 7-1, a second reflecting mirror 7-2, a third reflecting mirror 9, a first plano-concave cylindrical lens 8-1, a second plano-concave cylindrical lens 8-2, a first light-shielding plate 14-1, a second light-shielding plate 14-2, and a third light-shielding plate 14-3. When the first light-shielding plate 14-1, the second light-shielding plate 14-2, and the third light-shielding plate 14-3 are not placed, the coherent light generated by the laser 4 is split by the beam splitter 5 into upper and lower laser beams; wherein the upper laser beam passes through the convex lens 6. After the light is converged by mirror 6, it illuminates the first reflecting mirror 7-1. After the laser beam is expanded by the first plano-concave cylindrical lens 8-1, it passes through the third reflecting mirror 9 and illuminates the object under test. The lower laser beam is expanded by the second plano-concave cylindrical lens 8-2 and then passes through the second reflecting mirror 7-2 to illuminate the object under test. This generates two coherent transmitted and reflected beams simultaneously. The original speckle image sequence with shared transmission and reflection can be acquired by the image acquisition module to achieve laser speckle contrast imaging with shared transmission and reflection.

[0046] The beam splitter 5 is a cylindrical beam splitter prism coated with a near-infrared anti-reflection film; the first reflector 7-1, the second reflector 7-2, and the third reflector 9 are all square reflectors.

[0047] The platform 10 is used to place the object to be tested.

[0048] The image acquisition module includes a camera 11, an adjustable microscope tube 12, and a microscope objective 13; wherein the distance between the camera 11 and the microscope objective 13 is adjusted by rotating the adjustable microscope tube 12 to acquire a clear sequence of raw speckle images. The camera 11 is a CMOS camera.

[0049] When the second light-shielding plate 14-2 is placed, or when the first light-shielding plate 14-1 and the third light-shielding plate 14-3 are placed simultaneously, there is no coherent transmitted light or reflected light in the system.

[0050] When only the first light-shielding plate 14-1 is placed, the system contains only coherent transmitted light, which can acquire the original speckle image sequence of transmission and realize transmission laser speckle contrast imaging.

[0051] When only the third light-shielding plate 14-3 is placed, the system contains only coherent reflected light, which can acquire the original speckle image sequence of reflection and realize reflective laser speckle contrast imaging.

[0052] The display platform 10 has a light-transmitting hole at its geometric center, and the size and shape of the light-transmitting hole are adjustable.

[0053] The angle between the first reflector 7-1 and the second reflector 7-2 and the horizontal line is 45 degrees.

[0054] The center of the laser emitted by the laser 4 is on the same horizontal line as the geometric center of the beam splitter 5, the second plano-concave cylindrical lens 8-2, and the second reflector 7-2.

[0055] The geometric centers of the first reflector 7-1, the first plano-concave cylindrical lens 8-1, and the third reflector 9 are located on the same horizontal line.

[0056] The geometric centers of the beam splitter 5, the convex lens 6, and the first reflecting mirror 7-1 are located on the same vertical line.

[0057] The geometric centers of the second reflecting mirror 7-2, the stage 10, and the microscope objective 13 are located on the same vertical line.

[0058] The convex lens 6 is located between the beam splitter 5 and the first reflecting mirror 7-1. The convex lens 6 can be moved up and down to adjust the distance between the convex lens 6 and the beam splitter 5 and the first reflecting mirror 7-1.

[0059] The third reflecting mirror 9 can rotate freely according to the horizontal central axis, thus forming different angles with the horizontal line.

[0060] The stage 10 is located between the second reflecting mirror 7-2 and the microscope objective 13. The stage 10 can be moved up and down to adjust the distance between the stage 10 and the second reflecting mirror 7-2 and the microscope objective 13.

[0061] The camera 11 and the microscope objective 13 are assembled into a whole through the adjustable lens tube 12, and can be moved up and down as a whole.

[0062] The length of the third reflecting mirror 9 is set to half of the length of the third reflecting mirror 9. Half the length of the camera 11 is Therefore, the horizontal distance between the geometric centers of the third reflecting mirror 9 and the camera 11 should be greater than [the horizontal distance between them]. .

[0063] Figure 2 and Figure 3 The image shows a laser speckle contrast imaging system with both transmissive and reflective capabilities placed in the front and side views of one embodiment, as shown. Figure 2 and Figure 3As shown, the laser mounting base 4-0, beam splitter mounting base 5-0, first light shield 14-1, second light shield 14-2, third light shield 14-3, second plano-concave cylindrical lens mounting base 8-2-0, connecting sleeve 19, second reflector mounting base 7-2-0, convex lens mounting base 6-0, first reflector mounting base 7-1-0, first plano-concave cylindrical lens mounting base 8-1-0, third reflector mounting base 9-0, and adjustable light-passing aperture light shield 10-1 are all 3D printed.

[0064] The first system bracket 17, the second system bracket 18, the shelf 10, the upper and lower movable platform 15, and the fixed bracket 16 are all made of aluminum alloy by sandblasting and oxidation.

[0065] Install laser 4 onto laser mounting base 4-0, install beam splitter 5 onto beam splitter mounting base 5-0, install convex lens 6 onto convex lens mounting base 6-0, install first reflector 7-1 onto first reflector mounting base 7-1-0, install second reflector 7-2 onto second reflector mounting base 7-2-0, install third reflector 9 onto third reflector mounting base 9-0 (the third reflector 9 can rotate freely according to the horizontal central axis and form different angles with the horizontal line), install first plano-concave cylindrical lens 8-1 onto first plano-concave cylindrical lens mounting base 8-1-0, and install second plano-concave cylindrical lens 8-2 onto second plano-concave cylindrical lens mounting base 8-2-0.

[0066] Then, the laser mounting base 4-0 is fixed to the lower left side of the second system bracket 18. The beam splitter mounting base 5-0 and the second plano-concave cylindrical lens mounting base 8-2-0 are fixed sequentially to the right side of the second system bracket 18. The right side of the second plano-concave cylindrical lens mounting base 8-2-0 is fixed to the left outer side of the first system bracket 17. The connecting sleeve 19 and the second reflector mounting base 7-2-0 are fixed sequentially to the left inner side of the first system bracket 17, so that the center of the laser emitted by the laser 4 is on the same horizontal line as the geometric center of the beam splitter 5, the second plano-concave cylindrical lens 8-2, and the second reflector 7-2.

[0067] The first reflector mounting base 7-1-0 and the first plano-concave cylindrical lens mounting base 8-1-0 are sequentially fixed to the upper right side of the second system bracket 18. The right side of the first plano-concave cylindrical lens mounting base 8-1-0 is fixed to the left outer side of the first system bracket 17. The third reflector mounting base 9-0 is fixed to the left inner side of the first system bracket 17, so that the geometric centers of the first reflector 7-1, the first plano-concave cylindrical lens 8-1 and the third reflector 9 are located on the same horizontal line.

[0068] The convex lens mounting base 6-0 is fixed to the right side of the second system bracket 18 and is located between the beam splitter mounting base 5-0 and the first reflector mounting base 7-1-0. The convex lens mounting base 6-0 can move up and down, so that the geometric centers of the beam splitter 5, the convex lens 6 and the first reflector 7-1 are located on the same vertical line.

[0069] The first light-shielding plate 14-1, the second light-shielding plate 14-2, and the third light-shielding plate 14-3 are installed on the beam splitter mounting base. The optical path in the system is changed by changing the placement of the light-shielding plates.

[0070] The camera 11, adjustable lens tube 12, and microscope objective 13 are assembled into a whole and mounted on the vertical moving stage 15. The vertical moving stage 15 is mounted on the fixed bracket 16, and the fixed bracket 16 is mounted on the right inner side of the first system bracket 17, so that the camera 11 can move up and down, while the geometric center of the microscope objective 13 and the second reflecting mirror 7-2 are located on the same vertical line.

[0071] The adjustable light-transmitting aperture shield 10-1 is installed on the stage 10, which can change the size and shape of the light-transmitting aperture. The stage 10 is installed on the first system bracket 17. The stage 10 is located between the microscope objective 13 and the second mirror mounting base 7-2-0, and the geometric center of the stage 10 is on the same vertical line as the geometric center of the microscope objective 13 and the second mirror 7-2. The stage 10 can move up and down.

[0072] Corresponding to the above system, the present invention also provides a laser speckle contrast imaging method for transmissive and reflective imaging, comprising the following operations:

[0073] S1, Adjust the laser controller 1 to make the laser 4 produce a suitable laser power output.

[0074] S2, firstly, without placing the first light-shielding plate 14-1, the second light-shielding plate 14-2, and the third light-shielding plate 14-3, coherent transmitted light and reflected light simultaneously exist in the system. The coherent transmitted light and reflected light simultaneously illuminate the object to be tested placed on the stage 10, and the camera 11 acquires a clear sequence of original speckle images with both transmission and reflection. .

[0075] S3, Set the sequence of original speckle images to be acquired. The resolution size is The frame rate is z, and the selected sliding window size is... The sliding space window iterates through the range of pixel coordinates (x, y, z); based on the original speckle image sequence... Calculate spatial speckle contrast image .

[0076] In this embodiment, the selection is... .

[0077] .

[0078] Where x takes values ​​in the range of 1 / 2. The range of values ​​for y is The range of values ​​for z is .

[0079] Figure 4 To create the cross-sectional diagram of the phantom, an epoxy resin model was made, and the epoxy resin was mixed with... Titanium dioxide and Indian ink simulates the dermis; using inner diameter , outer diameter The capillary glass tubes simulate blood vessels, and are placed at an angle; the width of the fabricated phantom is... Thickness is A 3% fat emulsion solution was injected into a capillary glass tube at a speed of 5 mm / s using a syringe pump to simulate blood, and the original speckle image sequence was obtained by a camera.

[0080] Figure 5 The left image shows a transmission laser speckle contrast image obtained using the spatial speckle contrast imaging method. Figure 5 The middle image shows a reflective laser speckle contrast image obtained using the spatial speckle contrast imaging method; Figure 5 The right image is a laser speckle contrast image with both transmission and reflection capabilities obtained using the method of this invention. Figure 5 It can be seen that the laser speckle contrast image with both transmission and reflection has a deeper imaging depth than the reflective laser speckle contrast image, and has better imaging quality of shallow blood vessels than the transmission laser speckle contrast image; this proves that the method of the present invention can improve the imaging depth of blood vessels while ensuring the imaging quality of shallow blood vessels.

[0081] The various embodiments in this specification are described in a progressive manner, with each embodiment focusing on its differences from other embodiments. Similar or identical parts between embodiments can be referred to interchangeably. For the systems disclosed in the embodiments, since they correspond to the methods disclosed in the embodiments, the descriptions are relatively simple; relevant parts can be referred to the method section.

[0082] This document uses specific examples to illustrate the principles and implementation methods of the present invention. The descriptions of the above embodiments are only for the purpose of helping to understand the method and core ideas of the present invention. Furthermore, those skilled in the art will recognize that, based on the ideas of the present invention, there will be changes in the specific implementation methods and application scope. Therefore, the content of this specification should not be construed as a limitation of the present invention.

Claims

1. A laser speckle contrast blood flow imaging system with both transmissive and reflective capabilities, characterized in that, include: Laser source module, optical path module, stage, and image acquisition module; The laser source module is used to generate coherent light with a set laser power to the optical path module; The platform is used to place the object to be tested. The optical path module includes: a beam splitter, a convex lens, a first reflecting mirror, a second reflecting mirror, a third reflecting mirror, a first plano-concave cylindrical lens, a second plano-concave cylindrical lens, a first light-shielding plate, a second light-shielding plate, and a third light-shielding plate. When the first, second, and third light-shielding plates are not placed, the coherent light generated by the laser source module is split by the beam splitter into an upper laser beam and a lower laser beam. The upper laser beam is converged by the convex lens and then illuminates the first reflecting mirror, passing through the first plano-concave cylindrical lens. After the upper laser beam is expanded by the mirror, it passes through the third reflecting mirror and illuminates the object under test; the lower laser beam is expanded by the second plano-concave cylindrical lens and passes through the second reflecting mirror and illuminates the object under test; then the image acquisition module acquires the two coherent transmitted and reflected beams generated simultaneously to obtain the original speckle image sequence with shared transmission and reflection, realizing laser speckle contrast imaging with shared transmission and reflection; when the second light shield is placed or when the first and third light shields are placed simultaneously, there is no coherent transmitted and reflected light; When only the first light-shielding plate is placed, a sequence of original transmissive speckle images is acquired based on the generated coherent transmitted light, achieving transmissive laser speckle contrast imaging; when only the third light-shielding plate is placed, a sequence of original reflective speckle images is acquired based on the generated coherent reflected light, achieving reflective laser speckle contrast imaging; the convex lens is located between the beam splitter and the first reflecting mirror; the stage is located between the second reflecting mirror and the image acquisition module; The angle between the first reflector and the horizontal line is the same as the angle between the second reflector and the horizontal line; the center of the coherent light emitted by the laser source module is on the same horizontal line as the geometric center of the beam splitter, the second plano-concave cylindrical lens, and the second reflector; the geometric centers of the first reflector, the first plano-concave cylindrical lens, and the third reflector are on the same horizontal line; the geometric centers of the beam splitter, the convex lens, and the first reflector are on the same vertical line. The image acquisition module is used to acquire transmission-type raw speckle image sequences, reflection-type raw speckle image sequences, and transmission-reflection combined raw speckle image sequences.

2. The laser speckle contrast imaging system for both transmission and reflection as described in claim 1, characterized in that, The laser source module includes: a laser controller and a laser; The laser controller and the laser are connected via a current drive line and a temperature control line; The laser, under the control of the laser controller, is used to generate coherent light with a set laser power.

3. The laser speckle contrast imaging system for both transmission and reflection as described in claim 1, characterized in that, The image acquisition module includes: a camera, an adjustable lens barrel, and a microscope objective; The adjustable lens barrel is used to adjust the distance between the camera and the microscope objective by rotating it; The geometric centers of the second reflecting mirror, the stage, and the microscope objective are located on the same vertical line.

4. The laser speckle contrast blood flow imaging system with both transmissivity and reflectivity as described in claim 3, characterized in that, Half the length of the third reflecting mirror is Half the length of the camera is The horizontal distance between the third reflecting mirror and the geometric center of the camera is greater than .

5. A laser speckle contrast blood flow imaging system with both transmissivity and reflectivity as described in claim 1, characterized in that, The display stand has a light-transmitting hole at its geometric center.

6. The laser speckle contrast blood flow imaging system with both transmissivity and reflectivity as described in claim 1, characterized in that, The third reflector is used to rotate freely according to the horizontal central axis, thereby forming different angles with the horizontal line.

7. A laser speckle contrast imaging method using both transmissive and reflective methods, characterized in that, The laser speckle contrast blood flow imaging system with both transmissivity and reflectivity as described in any one of claims 1-6 is characterized by comprising: Adjust the laser controller to make the laser generate coherent light with a set laser power to the optical path module; When the first, second, and third light-shielding plates are not placed, the camera is used to collect the two coherent transmitted and reflected light simultaneously to obtain the original speckle image sequence with shared transmission and reflection. The spatial speckle contrast image is calculated based on the original speckle image sequence with shared perspective and reflection.

8. The laser speckle contrast imaging method for transmissive and reflective imaging according to claim 7, characterized in that, The calculation of the spatial speckle contrast image based on the original speckle image sequence shared by perspective and reflection specifically includes: Using formula Determine spatial speckle contrast image ; in, The size of the sliding space window. Let z be the pixel coordinates, and z ranges from [1, Z], where Z is the original speckle image sequence. Frame count, The value range is [1, row- +1], row is the original speckle image sequence the number of rows, The range of values ​​for is [1, col- +1], col is the original speckle image sequence The number of columns.

Images