A bactericidal composition containing resveratrol

Abstract

The present invention belongs to the technical field of pesticides, and particularly relates to a bactericidal composition containing resveratrol. A bactericidal composition containing resveratrol, the active ingredients of which are compounded by magnolol, carvacrol or oregano oil and resveratrol. The present invention compounded magnolol, carvacrol or oregano oil and resveratrol in a certain mass, showing a synergistic effect on various pathogenic bacteria of plant fungal diseases, and can improve the control effect on plant diseases.

Description

Technical Field

[0001] The present invention belongs to the technical field of pesticides, and particularly relates to a bactericidal composition containing resveratrol. Background Art

[0002] Plant diseases are one of the important factors affecting crop yield and quality at present. According to the statistics of the Food and Agriculture Organization of the United Nations (FAO), the loss caused by diseases in cereal production is 10%, and the loss caused by diseases in cotton production is 12%. Moreover, the economic loss caused by pests and harmful organisms worldwide reaches up to 120 billion US dollars every year, which is equivalent to 1 / 2 of the total agricultural output value of China. However, due to the long-term and large-scale application of chemical pesticides, a series of prominent problems have occurred, such as residual pollution of agricultural products, the generation of drug resistance in pests and harmful organisms, the deterioration of the ecological environment, and the decline in the level of biodiversity.

[0003] Plant-derived pesticides refer to new pesticides directly or indirectly processed and synthesized from active substances with insecticidal or antibacterial effects extracted from plants, which cover active ingredients purified from plants, the plants themselves, and compounds synthesized after modifying the active structure. Plant-derived pesticides have attracted much attention and favor due to their advantages such as environmental protection, diverse biological activities, the difficulty for pests and harmful organisms to generate drug resistance, and safety to non-target organisms. At present, many plant-derived pesticide products have been registered for the prevention and control of pests and harmful organisms in production, such as matrine, tea cicada net, veratridine, etc.

[0004] Resveratrol, also known as pterostilbene, has the molecular formula of C 14 H 12Resveratrol, a non - flavonoid natural polyphenol compound, naturally exists in various plants such as grapes, peanuts, blueberries, and polygonum cuspidatum. It has various biological activities such as antioxidant, antibacterial and anti - inflammatory, anti - cardiovascular disease, and anti - tumor effects. Resveratrol has cis and trans isomers, and usually the trans configuration is dominant in nature. As a phytoalexin, resveratrol plays a protective role in plants, such as resisting ultraviolet radiation and preventing fungal infections. Existing studies have shown that due to its excellent functional activities such as antioxidant, antibacterial, anti - inflammatory, anti - tumor, and anti - cardiovascular in promoting human nutrition and health, resveratrol has been widely used in the fields of food, medicine, and daily chemicals. Existing studies have shown that resveratrol can inhibit the proliferation of mammalian hepatocytes by inhibiting glycolysis, and hepatocytes are also relatively sensitive to resveratrol. At the same time, Parekh et al. found that resveratrol inhibits the growth of human HepG2 liver cancer cells by promoting apoptosis. Liu et al. found that the combination of resveratrol and cisplatin can reduce glutamine uptake by decreasing the expression of ASCT2 and enhance the anti - tumor activity of cisplatin. In addition, Dai et al. found that resveratrol inhibits the malignant progression of hepatocellular carcinoma through the regulation of March1 - induced PTEN / Akt signal transduction. The research results further show that resveratrol can inhibit the expansion of mammalian cancer cells by inducing cancer cell apoptosis, inhibiting cancer cell growth, and enhancing the activity of chemotherapeutic drugs.

[0005] Currently, there is little research on resveratrol in terms of plant diseases. Our research group found that resveratrol has excellent antibacterial activity against Fusarium graminearum and F. pseudograminearum, and has strong antibacterial activity against Sclerotium rolfsii, Rhizoctonia solani, Fusarium incarnatum, F. equiseti, F. proliferatum, and Fusarium oxysporum, showing strong potential in preventing and controlling wheat scab, wheat basal rot, maize scab, and peanut root rot.

[0006] In order to further expand the control spectrum and improve the control effect on plant diseases, this study further carried out the binary compound research of resveratrol with candidate plant - derived fungicides magnolol, carvacrol, oregano oil, osthole, and allicin. The results showed that when resveratrol was compounded with candidate fungicides, it showed strong synergistic effects on Fusarium graminearum, F. pseudograminearum, Sclerotium rolfsii, Rhizoctonia solani, Fusarium incarnatum, F. equiseti, F. proliferatum, and Fusarium oxysporum, further indicating that resveratrol and its compound combinations have strong potential for preventing and controlling wheat scab, wheat basal rot, maize scab, and peanut root rot. This invention is of great significance for effectively preventing and controlling wheat scab, wheat basal rot, maize scab, and peanut root rot and further ensuring the safe production of wheat, maize, and peanuts.

[0007] The information disclosed in this background section is only intended to enhance the overall understanding of the background of the present invention and should not be regarded as an admission or any form of implication that this information constitutes the prior art already known to those of ordinary skill in the art. Summary of the Invention

[0008] The object of the present invention is to provide a bactericidal composition containing resveratrol, which can effectively inhibit the growth of pathogenic bacteria such as wheat basal rot, wheat scab, peanut root rot, peanut southern blight, and maize scab, effectively prevent and control various plant fungal diseases, and is of great significance for the high-quality and high-yield production of crops such as wheat, peanuts, and maize.

[0009] To achieve the above object, the present invention provides the following technical solutions:

[0010] A bactericidal composition containing resveratrol, the active ingredients of which are compounded by magnolol, carvacrol or oregano oil and resveratrol.

[0011] Preferably, the mass ratio of magnolol to resveratrol is 1 - 9:9 - 1.

[0012] Preferably, the mass ratio of carvacrol to resveratrol is 1 - 9:9 - 1.

[0013] Preferably, the mass ratio of oregano oil to resveratrol is 1 - 9:9 - 1.

[0014] The present invention also provides the use of the bactericidal composition containing resveratrol in the prevention and control of crop fungal diseases.

[0015] Preferably, the crop fungal diseases include wheat basal rot, wheat scab, peanut root rot, peanut southern blight, and maize scab.

[0016] Compared with the prior art, the present invention has the following beneficial effects:

[0017] The present invention compounded magnolol, carvacrol or oregano oil and resveratrol in a certain mass, showing a synergistic effect on various plant fungal disease pathogens, which can improve the control effect on plant diseases, provide data support for the effective prevention and control of wheat basal rot, wheat scab, peanut root rot, peanut southern blight and maize scab, and is of great significance for the high-quality and high-yield production of crops such as wheat, peanuts, and maize. Detailed Embodiments

[0018] The technical solutions of the present invention will be clearly and completely described below.

[0019] Example

[0020] 1. Test Strains

[0021] Table 1 Test strains

[0022]

[0023] 2. Test agents

[0024] 98% magnolol technical (Shanghai Aladdin Biochemical Technology Co., Ltd.), 92.5% carvacrol technical (Shanghai Yuanye Bio-Technology Co., Ltd.), 99% origanum oil technical (Jiangxi Hairui Natural Plant Co., Ltd.), 98% resveratrol technical (Shanghai Yuanye Bio-Technology Co., Ltd.).

[0025] After the test agents were dissolved, they were diluted into single-agent mother liquors with 0.1% Tween-80 aqueous solution. Multiple groups of ratios were set, and 5 mass concentration gradients were set for each single-agent mother liquor and the mixed agents of ratios according to the equal ratio method.

[0026] 3. Test methods

[0027] Add 9 mL of pre-melted PDA medium into a sterile conical flask. Quantitatively pipette 1 mL of the liquid medicine from low concentration to high concentration in turn and add them into the above conical flasks. After shaking well, pour them into a petri dish with a diameter of 9 cm to make drug-containing plates with corresponding concentrations. At the same time, set a treatment without the agent as a blank control, and treat 10 plates with the liquid medicine at each mass concentration. Use a punch with a diameter of 5 mm to cut out a mycelial disc at the edge of the test strain colony and inoculate it in the center of the drug-containing plate and the blank control plate. Cover the petri dish lid and place it in an incubator at 25 °C for cultivation. When the colony diameter of the blank control grows to about 2 / 3 of the petri dish diameter, measure the colony diameter by the cross method and calculate the inhibition rate of mycelial growth for different treatments.

[0028]

[0029] 4. Data analysis

[0030] Use DPS software for data statistical analysis to obtain the virulence regression equation and the virulence EC 50 value of the agent against the target pathogen, and calculate the co-toxicity coefficient (CTC) according to the Sun Yunpei method.

[0031] 5. Determination results

[0032] Evaluate the synergistic effect of the agent according to the calculated co-toxicity coefficient (CTC). When CTC ≤ 80, it is an antagonistic effect; when 80 < CTC < 120, it is an additive effect; when CTC ≥ 120, it is a synergistic effect. The results are shown in Table 2-10.

[0033] Table 2 Indoor bioactivity determination of the combination of magnolol and resveratrol against Sclerotium rolfsii

[0034] Drug Name and Ratio <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC Magnolol 16.1248 100.0000 -- -- Resveratrol 139.9665 11.5205 -- -- Magnolol 9 : Resveratrol 1 44.1939 36.4865 91.1520 40.0282 Magnolol 8 : Resveratrol 2 51.5671 31.2695 82.3041 37.9927 Magnolol 7 : Resveratrol 3 72.4176 22.2664 73.4561 30.3125 Magnolol 6 : Resveratrol 4 91.0151 17.7166 64.6082 27.4216 Magnolol 5 : Resveratrol 5 142.3682 11.3261 55.7602 20.3122 Magnolol 4 : Resveratrol 6 20.9402 77.0040 46.9123 164.1447 Magnolol 3 : Resveratrol 7 35.0952 45.9459 38.0643 120.7059 Magnolol 2 : Resveratrol 8 34.0924 47.2973 29.2164 161.8864 Magnolol 1 : Resveratrol 9 31.1835 51.7094 20.3684 253.8704

[0035] As can be seen from Table 2, when magnolol and resveratrol are compounded, when the mass ratio is 9 - 5:1 - 5, the co-toxicity coefficient of the compound combination against Sclerotium rolfsii is less than 80, showing an antagonistic effect; when the mass ratio is 4 - 1:6 - 9, the co-toxicity coefficient of the compound combination against Sclerotium rolfsii is greater than 120, showing a synergistic effect.

[0036] Table 3 Indoor bioactivity determination of the compound of carvacrol and resveratrol against Sclerotium rolfsii

[0037]

[0038]

[0039] As can be seen from Table 3, when carvacrol and resveratrol are compounded, within the mass ratio range of 1 - 9:9 - 1, the co-toxicity coefficients of all tested compound combinations against Sclerotium rolfsii are greater than 120, showing a synergistic effect.

[0040] Table 4 Indoor bioactivity determination of the compound of oregano oil and resveratrol against Sclerotium rolfsii

[0041] Drug Name and Ratio <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC Oregano Oil 52.2065 100.0000 -- -- Resveratrol 139.9665 37.2993 -- -- Oregano Oil 9 : Resveratrol 1 7.4581 699.9973 93.7299 746.8237 Oregano Oil 8 : Resveratrol 2 4.0786 1280.0103 87.4599 1463.5404 Oregano Oil 7 : Resveratrol 3 7.9101 659.9980 81.1898 812.9077 Oregano Oil 6 : Resveratrol 4 21.7527 240.0001 74.9197 320.3430 Oregano Oil 5 : Resveratrol 5 43.5054 120.0000 68.6496 174.8007 Oregano Oil 4 : Resveratrol 6 14.5018 360.0001 62.3796 577.1123 Oregano Oil 3 : Resveratrol 7 10.4413 500.0000 56.1095 891.1147 Oregano Oil 2 : Resveratrol 8 13.0516 400.0008 49.8394 802.5790 Oregano Oil 1 : Resveratrol 9 15.7386 331.7099 43.5694 761.3377

[0042] As can be seen from Table 4, when oregano oil and resveratrol are compounded, within the mass ratio range of 1 - 9:9 - 1, the co-toxicity coefficients of all tested compound combinations against Sclerotium rolfsii are greater than 120, showing a synergistic effect; especially when the mass ratio is 8:2, the co-toxicity coefficient of the compound combination against Sclerotium rolfsii reaches 1463.5404, and the synergistic effect is the most significant.

[0043] Table 5 Indoor bioactivity determination of the compound of magnolol and resveratrol against Fusarium graminearum

[0044]

[0045]

[0046] As can be seen from Table 5, when magnolol and resveratrol are compounded, when the mass ratio is 8:2, 6:4, 5:5 or 3:7, the co-toxicity coefficient of the compound combination against Fusarium graminearum is less than 80, showing an antagonistic effect; when the mass ratio is 7:3 or 2:8, the co-toxicity coefficient of the compound combination against Fusarium graminearum is between 80 - 120, showing an additive effect; the co-toxicity coefficients of the other compound combinations against Fusarium graminearum are all greater than 120, showing a synergistic effect.

[0047] Table 6 Indoor bioactivity determination of the compound of carvacrol and resveratrol against Fusarium graminearum

[0048] Drug Name and Ratio <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC Carvacrol 24.9855 100.0000 -- -- Resveratrol 29.1159 85.8139 -- -- Carvacrol 9 : Resveratrol 1 15.9467 156.6813 98.5814 158.9360 Carvacrol 8 : Resveratrol 2 23.8832 104.6154 97.1628 107.6702 Carvacrol 7 : Resveratrol 3 37.7688 66.1538 95.7442 69.0943 Carvacrol 6 : Resveratrol 4 30.0751 83.0770 94.3256 88.0748 Carvacrol 5 : Resveratrol 5 32.2632 77.4427 92.9070 83.3551 Carvacrol 4 : Resveratrol 6 33.8345 73.8462 91.4884 80.7165 Carvacrol 3 : Resveratrol 7 37.7688 66.1538 90.0698 73.4473 Carvacrol 2 : Resveratrol 8 42.9337 58.1955 88.6511 65.6456 Carvacrol 1 : Resveratrol 9 36.3434 68.7484 87.2325 78.8105

[0049] As can be seen from Table 6, when carvacrol and resveratrol are compounded, and the mass ratio is 9:1, the co-toxicity coefficient of the compound combination against Fusarium graminearum is greater than 120, showing a synergistic effect.

[0050] Table 7 Indoor bioactivity determination of the compound of oregano oil and resveratrol against Fusarium graminearum

[0051] Drug Name and Ratio <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC Oregano Oil 27.8974 100.0000 -- -- Resveratrol 29.1159 95.8150 -- -- Oregano Oil 9 : Resveratrol 1 9.1116 306.1745 99.5815 307.4613 Oregano Oil 8 : Resveratrol 2 7.0868 393.6530 99.1630 396.9757 Oregano Oil 7 : Resveratrol 3 8.6483 322.5767 98.7445 326.6781 Oregano Oil 6 : Resveratrol 4 9.4491 295.2387 98.3260 300.2651 Oregano Oil 5 : Resveratrol 5 10.8564 256.9673 97.9075 262.4593 Oregano Oil 4 : Resveratrol 6 15.0073 185.8922 97.4890 190.6802 Oregano Oil 3 : Resveratrol 7 13.4276 207.7616 97.0705 214.0317 Oregano Oil 2 : Resveratrol 8 22.1848 125.7501 96.6520 130.1060 Oregano Oil 1 : Resveratrol 9 13.1380 212.3413 96.2335 220.6522

[0052] As can be seen from Table 7, when oregano oil and resveratrol are compounded, within the mass ratio range of 1-9:9-1, the co-toxicity coefficients of all tested compound combinations against Fusarium graminearum are greater than 120, showing a synergistic effect.

[0053] Table 8 Indoor bioactivity determination of the compound of magnolol and resveratrol against Fusarium proliferatum

[0054] Drug Name and Ratio <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC Magnolol 6.2200 100.0000 -- -- Resveratrol 105.6220 5.8889 -- -- Magnolol 9 : Resveratrol 1 10.8799 57.1696 90.5889 63.1089 Magnolol 8 : Resveratrol 2 8.1663 76.1667 81.1778 93.8270 Magnolol 7 : Resveratrol 3 7.8327 79.4107 71.7667 110.6512 Magnolol 6 : Resveratrol 4 11.7699 52.8467 62.3556 84.7505 Magnolol 5 : Resveratrol 5 19.3511 32.1429 52.9445 60.7106 Magnolol 4 : Resveratrol 6 13.7891 45.1081 43.5334 103.6173 Magnolol 3 : Resveratrol 7 12.0109 51.7863 34.1222 151.7670 Magnolol 2 : Resveratrol 8 18.7327 33.2040 24.7111 134.3684 Magnolol 1 : Resveratrol 9 22.9138 27.1452 15.3000 177.4193

[0055] As can be seen from Table 5, when magnolol and resveratrol are compounded, within the mass ratio range of 3-1:7-9, the co-toxicity coefficients of the compound combination against Fusarium proliferatum are greater than 120, showing a synergistic effect.

[0056] Table 9 Indoor bioactivity determination of the compound of carvacrol and resveratrol against Fusarium proliferatum

[0057] Drug Name and Ratio <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC Carvacrol 29.8643 100.0000 -- -- Resveratrol 105.6220 28.2747 -- -- Carvacrol 9 : Resveratrol 1 11.9457 250.0004 92.8275 269.3173 Carvacrol 8 : Resveratrol 2 9.1890 325.0005 85.6549 379.4300 Carvacrol 7 : Resveratrol 3 12.5744 237.5008 78.4824 302.6166 Carvacrol 6 : Resveratrol 4 14.8600 200.9711 71.3099 281.8278 Carvacrol 5 : Resveratrol 5 16.8815 176.9055 64.1373 275.8229 Carvacrol 4 : Resveratrol 6 11.3769 262.4995 56.9648 460.8098 Carvacrol 3 : Resveratrol 7 13.6665 218.5219 49.7923 438.8670 Carvacrol 2 : Resveratrol 8 18.9476 157.6152 42.6198 369.8172 Carvacrol 1 : Resveratrol 9 21.5327 138.6928 35.4472 391.2655

[0058] As can be seen from Table 9, when carvacrol and resveratrol are compounded, within the mass ratio range of 1-9:9-1, the co-toxicity coefficients of all tested compound combinations against Fusarium proliferatum are greater than 120, showing a synergistic effect.

[0059] Table 10 Indoor bioactivity determination of the compound of oregano oil and resveratrol against Fusarium proliferatum

[0060]

[0061]

[0062] As can be seen from Table 10, when oregano oil and resveratrol are compounded, within the mass ratio range of 1-9:9-1, the co-toxicity coefficients of all tested compound combinations against Fusarium proliferatum are greater than 120, showing a synergistic effect.

[0063] In summary, the present invention combines magnolol, carvacrol or oregano oil with resveratrol in a certain mass ratio, showing a synergistic effect on various pathogenic bacteria of plant fungal diseases, which can improve the control effect on plant diseases, providing data support for the effective prevention and control of wheat basal rot, wheat scab, peanut root rot, peanut southern blight and maize scab, and is of great significance for the high-quality and high-yield of crops such as wheat, peanut and maize.

[0064] The foregoing description of the specific exemplary embodiments of the present invention is for purposes of illustration and exemplification. These descriptions are not intended to limit the invention to the precise forms disclosed, and obviously, many changes and variations are possible in light of the above teachings. The purpose of selecting and describing the exemplary embodiments is to explain the specific principles of the present invention and its practical applications, so that those skilled in the art can implement and utilize the various different exemplary embodiments of the present invention, as well as various different selections and changes. The scope of the present invention is intended to be defined by the claims and their equivalents.

Claims

1. Use of a resveratrol-containing bactericidal composition in fungal diseases of crops, wherein the fungal diseases of crops are caused by Sclerotium rolfsii, Fusarium graminearum or Fusarium proliferatum, characterized in that, The active ingredients of the resveratrol-containing bactericidal composition are composed of magnolol, carvacrol or oregano oil and resveratrol in combination; When preventing and controlling fungal diseases of crops caused by Sclerotium rolfsii, the mass ratio of magnolol to resveratrol is 4 - 1:6 - 9; the mass ratio of carvacrol to resveratrol is 1 - 9:9 - 1; the mass ratio of oregano oil to resveratrol is 1 - 9:9 - 1; When preventing and controlling fungal diseases of crops caused by Fusarium graminearum, the mass ratio of magnolol to resveratrol is 9:1 or 1:9; the mass ratio of carvacrol to resveratrol is 9:1; the mass ratio of oregano oil to resveratrol is 1 - 9:9 - 1; When preventing and controlling fungal diseases of crops caused by Fusarium proliferatum, the mass ratio of magnolol to resveratrol is 3 - 1:7 - 9; the mass ratio of carvacrol to resveratrol is 1 - 9:9 - 1; the mass ratio of oregano oil to resveratrol is 1 - 9:9 - 1.

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