A method for raising high-quality Wagyu beef

Abstract

The present invention relates to a method for breeding high-quality Wagyu cattle, and belongs to the field of agricultural breeding technology. By naturalizing purebred Wagyu cattle, they are given better immunity and resistance, are less prone to illness, and achieve a better breeding state; and by combining different breeding methods for bulls, cows, and calves, and customized feeding methods, the taste of beef is unified to ensure quality. Tannins are encapsulated using encapsulating materials to improve the bitter taste of tannins. By controlling the light intensity and illumination time, reducing the light strobe, and combining medicated baths, massages, and fragrant environments, the stress caused by the environment to cattle is reduced, thereby increasing the food intake of cattle. The preparation of long-acting antibacterial agents can improve conditions such as depression caused by cattle living in cowsheds for a long time while inhibiting bacteria.

Description

Technical Field

[0001] The present invention relates to the technical field of agricultural breeding, and in particular to a breeding method for high-quality Wagyu cattle. Background Art

[0002] Wagyu beef is recognized worldwide as the highest quality, boasting distinct marbled meat known as "marbled meat." Its juicy, tender meat and unique flavor are complemented by its rich content of sarcosine, vitamins B6 and B12, carnitine, linoleic acid, and glutamic acid, along with essential trace elements such as protein, calcium, magnesium, iron, and zinc. Its muscle fat is also very low in saturated fatty acids, contributing to its numerous health benefits and high nutritional value. This makes Wagyu beef a national treasure in Japan, yet it commands a high price in Western European markets.

[0003] However, due to the immaturity of breeding technology and the lack of scientific feeding methods, resources are wasted. During feeding, the protein ingested by ruminants is degraded by microorganisms in the rumen and cannot be fully absorbed and utilized by the small intestine and other posterior digestive tracts, reducing its biological value. The general treatment method is to use formaldehyde to treat the feed, but this may cause weak toxicity.

[0004] Furthermore, even among Wagyu cattle raised on the same pasture, there is a significant difference in the texture and taste of the meat between male and female cattle. Male beef contains coarser fibers, resulting in a firmer texture and a better taste. In some markets, there is even a significant price difference between male and female beef. Summary of the Invention

[0005] The object of the present invention is to provide a method for breeding high-quality Wagyu cattle to solve the problems raised in the background technology. By naturalizing purebred Wagyu cattle, they have better immunity and resistance, are less likely to get sick, and achieve a better breeding state; and by combining different breeding methods for bulls, cows, and calves, and customized feeding methods, the taste of beef is unified to ensure quality. Tannins are encapsulated using encapsulating materials to improve the bitter taste of tannins. By controlling the light intensity and illumination time, reducing the light strobe, and combining medicated baths, massages, and fragrant environments, the stress caused by the environment to cattle is reduced, thereby increasing the food intake of cattle. The preparation of long-acting antibacterial agents can improve the depression and other conditions caused by cattle living in cowsheds for a long time while inhibiting bacteria.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] A method for raising high-quality Wagyu cattle comprises the following steps:

[0008] (1) Seed selection: The breeding base purchases imported foundation cows, selects purebred Japanese black-haired Wagyu, Japanese red-haired Wagyu, Japanese short-horned Wagyu, Japanese hornless Wagyu and their hybrids, and implants two Wagyu embryos into the bodies of imported foundation cows. After a gestation period of 9-10 months and a breeding period of 28-30 months, an operation cycle is completed. Among them, the imported foundation cows are eliminated after two pregnancies, and the Wagyu cattle are naturalized and bred during the breeding period;

[0009] (2) Cowshed setup: Choose an area with a slope of 30°, good drainage, sheltered from the wind and facing the sun, quiet environment, convenient transportation, sufficient water and electricity, and low groundwater level. Set up the cowshed in an east-west direction and facing south. Prepare sunshade nets in summer and roller blinds in winter. Put down the sunshade nets in summer and roll up the roller blinds around to form a sweeping wind. Set up feed troughs, water troughs and air conditioners, set up underground drainage, and set up 24V low-voltage DC power supply lighting. The illumination of each area is 50Lux, with 16 hours of light and 8 hours of darkness per day.

[0010] (3) Daily feeding: Cows, bulls, and calves were fed with customized feeds. Cows and bulls were fed twice a day, each feeding 2.0-2.5% of their body weight. Calves were fed ad libitum. The cattle were marketed when their weight reached 800±25kg.

[0011] (4) Cowshed management: Every morning, after feeding for 30-60 minutes, the cattle are arranged to walk in an open area. Bulls walk for 45-60 minutes, and cows walk for 60-90 minutes. The cowshed is disinfected and treated with a long-lasting antibacterial agent once a week. Deworming is done 2-3 times a year. Music is played in the cowshed for 2 hours every day. The cattle are massaged with a brush every week. The cattle are given a medicated bath by spraying every month.

[0012] The customized feed includes the following components:

[0013] The customized feed for cows is: 80-105 parts of forage, 1-10 parts of compound plant extracts, 0.1-1 parts of compound vitamins, 30-60 parts of corn, 10-20 parts of beetroot, 20-30 parts of soybeans, and 10-20 parts of feed additives;

[0014] The customized feed for bulls is 100-150 parts of forage, 1-10 parts of compound plant extracts, 0.1-1 parts of compound vitamins, 20-30 parts of corn, 5-10 parts of beetroot, 10-20 parts of soybean meal, and 10-20 parts of feed additives;

[0015] The feed additive is a nanoparticle with plant protein as the wall material and polyphenols as the core material, and the forage grass is at least one of golden clover, leaf grass, barley grass and alfalfa.

[0016] Furthermore, in step (1), the specific method of naturalization is:

[0017] A1. Clean the selected Wagyu beef, disinfect it in vitro, and perform a shallow enema under a sterile environment until all feces in the rectum are removed, thereby obtaining a cleansed Wagyu beef.

[0018] A2. Select the native Mishima cattle breed from Mishima, Hagi City, Yamaguchi Prefecture, and collect fresh feces to obtain wild Wagyu feces;

[0019] A3. Add wild Wagyu feces to the drinking water of cleansed Wagyu cattle, raise the cleansed Wagyu cattle, provide them with food, and allow them to eat freely. After 1-2 weeks, naturalized Wagyu cattle will be obtained.

[0020] Furthermore, in step A1, the selected Wagyu cattle weigh 150kg-200kg.

[0021] Furthermore, the feed additive is prepared by the following steps:

[0022] B1. Disperse the plant protein in deionized water and place it in 20 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer at pH 7.0 for 2 hours to ensure complete dissolution. Then, add the polyphenols. Heat at 80°C for 30 minutes, cool in a cold water bath, and store at 4°C overnight to form a thermally cross-linked protein-polyphenol gel.

[0023] B2. Disperse the protein-polyphenol gel in 70 vol% HEPES buffer for 5 minutes, degas the large gel particle dispersion at 2200 rpm for 1 minute, and then homogenize the protein dispersion three times to obtain a feed additive.

[0024] Furthermore, the plant protein is at least one of pea protein concentrate and potato protein isolate.

[0025] Furthermore, the polyphenol substance is one of quercetin, tea polyphenols and tannic acid.

[0026] It should be noted that quercetin and tea polyphenols as flavonoids have strong antibacterial and anti-inflammatory effects, tannic acid can increase the content of rumen protein in cattle, reduce diarrhea symptoms, increase digestive tract enzyme activity, enhance intestinal structure and mucosal immune function, and improve production performance; considering the astringency of plant protein and polyphenols, and the poor hydration ability of plant protein leading to dry mouth, the plant protein is hydrated with water and thermally gelled by hydrophobic interaction, hydrogen bond and disulfide bond-based covalent cross-linking without the addition of any cross-linking agent. Natural plant protein acts as a connecting particle point in a highly permeable hydrogel network, which is then converted into a gel and then formed into particles by homogenization; in addition, polyphenols form insoluble precipitates with plant protein through hydrophobic interaction and hydrogen bonds, and then the plant protein encapsulates the polyphenols after forming a gel.

[0027] Furthermore, the composite plant extract comprises a Cyperus multicephalus extract and a Rosa roxburghii extract.

[0028] Furthermore, the roxburghii extract is prepared by comprising the following steps:

[0029] 5 g of roxburghii seeds were weighed and manually ground using a mortar and pestle. To defat the seeds, the fine powder was soaked in n-hexane at a ratio of 1:10 and allowed to react at room temperature for 90 minutes. The supernatant was removed, and the remaining powder was dried on a 40°C hot plate for 2 hours. The remaining powder was then soaked in anhydrous ethanol at a ratio of 1:10 at room temperature for 24 hours. The supernatant was then collected in a 50 ml glass beaker and kept on a hot plate at 40°C until the ethanol completely evaporated. This yielded the roxburghii extract.

[0030] It should be noted that: in the present invention, the roxburgh roxburgh extract is added to the bull's diet, and the roxburgh roxburgh extract can increase the bull's sperm activity and quality, increase the embryo survival rate during the breeding process, and facilitate better reproduction of Wagyu cattle.

[0031] Furthermore, the extract of Cyperus multiflorus comprises the following steps:

[0032] Collect 5 g of multi-headed Pinellia oleracea, wash it with deionized water, and then treat it in a 90°C oven for 5 minutes. Use a mortar and pestle to grind the dried Pinellia oleracea into powder, and use 70% ethanol solution to extract it at room temperature for 3-5 hours. Then filter it, and then evaporate the filtrate by reduced pressure rotary evaporation to obtain the Pinellia oleracea extract.

[0033] It should be noted that the extract of Cyperus multicephalus has a variety of chemical components, including triterpenoid saponins, flavonoids, phenylpropanoids and steroids, which have pharmacological effects such as antioxidant, anti-inflammatory and antibacterial.

[0034] Furthermore, in step (4), the long-acting antibacterial agent is prepared by the following steps:

[0035] C1. Mix ethanol and raw propolis at 30°C for 2 hours, then ultrasonicate at 40 kHz and 30°C for 30 minutes, and then filter to collect the supernatant; centrifuge the supernatant at 45°C for 10 minutes, then store at 4°C to remove the condensed beeswax on the surface to obtain a propolis extract; mix the propolis extract with resveratrol and eugenol in equal proportions to obtain a core material, and store at -30°C for later use;

[0036] C2. Dissolve β-cyclodextrin in deionized water at room temperature, stir evenly, add gum arabic, and homogenize in a machine for 30 minutes. Raise the system temperature to 100°C, continue stirring for 20 minutes, then add the core material, stir the system at 450 rpm for 1 hour, lower the system temperature to room temperature, ultrasonically treat for 20 minutes, and then continue heating to 100°C. After reacting for 12 hours, collect the system solution, spray dry it, with a flow rate of 10 mL / min, a nozzle diameter of 1.5 mm, an inlet temperature of 135°C, and an outlet temperature of 85°C to obtain a long-acting antibacterial agent.

[0037] Furthermore, the mass ratio of β-cyclodextrin, gum arabic and core material is 4:2:3.

[0038] Compared with the prior art, the present invention has the following beneficial effects:

[0039] (1) In the technical solution of the present invention, Mishima cattle are wild Japanese cattle of Japanese origin, and are similar in size to the four common basic breeds of Japanese cattle. Compared with Japanese cattle raised for beef, this wild Japanese cattle has more possibilities of contacting microorganisms in the natural environment, including pathogens, allergens, parasites, etc., so that it has accumulated more antigen experience, better immunity and a more complete immune library. The domestication treatment of the raised Japanese cattle can make their intestines have similar microbial composition, better immunity and resistance, less prone to disease, and achieve a better breeding state. Moreover, the naturalization treatment can help the introduced breed to achieve terroir domestication when introducing Japanese cattle from different regions for breeding and selection, and can ensure their normal survival, growth, development and reproduction, and show their inherent characteristics and excellent production performance.

[0040] (2) In the technical solution of the present invention, a core material containing propolis extract, resveratrol, and eugenol is wrapped by using β-cyclodextrin and gum arabic to prepare an antibacterial sustained-release agent, which has a long-lasting antibacterial and antimicrobial effect in the breeding environment. In addition, eugenol is used, which has antidepressant and fragrant aroma, etc., and is matched with a low-voltage DC power supply to avoid flickering of light, reduce the stress of cattle to light, improve the breeding environment of the cowshed, increase the utilization rate of food, and help cattle grow better. It is also completely non-toxic and will not cause poisoning even if accidentally eaten by cattle.

[0041] (3) In the technical solution of the present invention, customized feeds are used for cows and bulls respectively, which increases the roughage intake of cows, increases the firmness of cow meat, and increases milk production during the production process of cows. The intramuscular fat content of bull meat is reduced to uniform the taste of beef and ensure quality. Calves are fed a free-feeding program, which provides them with richer and more frequent meals, increasing their nutrient digestibility and dry matter intake.

[0042] (4) In the technical solution of the present invention, polyphenols are wrapped in plant protein to form particles, thereby improving the palatability of plant protein and polyphenols. Secondly, the lubricity of plant protein is enhanced, making it easier for Wagyu cattle to swallow, increasing the cattle's food intake, and improving feed utilization. DETAILED DESCRIPTION

[0043] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts shall fall within the scope of protection of the present invention.

[0044] The reagents used in the following examples and comparative examples were all of reagent grade, pea protein concentrate: pea protein concentrate containing 85% protein (PPC, Nutralys S85 XF), potato protein isolate: product number ZR-1206, purchased from Xi'an Rui Biotechnology Co., Ltd.

[0045] In the present invention, the roxburghii extract is prepared by the following steps:

[0046] 5 g of roxburghii seeds were weighed and manually ground using a mortar and pestle. To defat the seeds, the fine powder was soaked in n-hexane at a ratio of 1:10 and allowed to react at room temperature for 90 minutes. The supernatant was removed, and the remaining powder was dried on a 40°C hot plate for 2 hours. The remaining powder was then soaked in anhydrous ethanol at a ratio of 1:10 at room temperature for 24 hours. The supernatant was then collected in a 50 ml glass beaker and kept on a hot plate at 40°C until the ethanol completely evaporated. This yielded the roxburghii extract.

[0047] The extract of Cyperus multiflorus comprises the following steps:

[0048] Collect 5 g of multi-headed Pinellia oleracea, wash it with deionized water, and then treat it in a 90°C oven for 5 minutes. Use a mortar and pestle to grind the dried Pinellia oleracea into powder, and use 70% ethanol solution to extract it at room temperature for 4 hours. Then filter it, and then evaporate the filtrate by reduced pressure rotary evaporation to obtain the Pinellia oleracea extract.

[0049] The long-acting antibacterial agent is prepared by the following steps:

[0050] C1. Mix ethanol and raw propolis at 30°C for 2 hours, then ultrasonicate at 40 kHz and 30°C for 30 minutes, and then filter to collect the supernatant; centrifuge the supernatant at 45°C for 10 minutes, then store at 4°C to remove the condensed beeswax on the surface to obtain a propolis extract; mix the propolis extract with resveratrol and eugenol in equal proportions to obtain a core material, and store at -30°C for later use;

[0051] C2. Dissolve 4 g of β-cyclodextrin in 20 mL of deionized water at room temperature, stir evenly, add 2 g of gum arabic, and homogenize in a machine for 30 minutes. Raise the system temperature to 100°C, continue stirring for 20 minutes, then add 3 g of core material, stir the system at 450 rpm for 1 hour, lower the system temperature to room temperature, ultrasonicate for 20 minutes, and then continue heating to 100°C. After reacting for 12 hours, collect the system solution, spray dry it at a flow rate of 10 mL / min, a nozzle diameter of 1.5 mm, an inlet temperature of 135°C, and an outlet temperature of 85°C to obtain a long-acting antibacterial agent. Example

[0052] The feed additive is prepared by the following steps:

[0053] B1. Disperse 1.5 g of pea protein concentrate in 10 mL of deionized water, place in 20 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer at pH 7.0 for 2 hours to ensure complete dissolution, and then add 0.5 g of quercetin; heat at 80°C for 30 minutes, cool in a cold water bath, and then store at 4°C overnight to form a thermally cross-linked protein-polyphenol gel;

[0054] B2. Disperse 2 g of protein-polyphenol gel in HEPES buffer for 5 minutes, degas the large gel particle dispersion at 2200 rpm for 1 minute, and then homogenize the protein dispersion three times to obtain a feed additive. Example

[0055] The difference between this embodiment and embodiment 1 is that quercetin is replaced by tea polyphenols, and the remaining steps and raw materials are the same as those in embodiment 1. Example

[0056] The difference between this embodiment and embodiment 1 is that quercetin is replaced by tannic acid, and the remaining steps and raw materials are the same as those in embodiment 1. Example

[0057] The difference between this embodiment and embodiment 1 is that pea protein concentrate is replaced by potato protein isolate, and the remaining steps and raw materials are the same as those in embodiment 1. Example

[0058] The difference between this embodiment and embodiment 1 is that 1.5 g of pea protein concentrate is replaced by 0.75 g of potato protein isolate plus 0.75 g of pea protein concentrate, and the remaining steps and raw materials are the same as those in embodiment 1.

[0059] The viscosity of the feed additives prepared in Examples 1-5 was tested. 1.5 g of the feed additives was dispersed in 20 mL of deionized water, stirred and mixed evenly, and the viscosity was measured using a viscometer. The control group used non-cross-linked pea protein concentrate or non-cross-linked potato protein isolate.

[0060] In the technical solution of the present invention, the viscosity of the plant protein is greatly reduced by preparing the plant protein into microgel particles. Example

[0061] The long-acting antibacterial agent is prepared by the following steps:

[0062] C1. Mix 80 mL of 70% ethanol and 10 mL of raw propolis at 30°C for 2 hours, then sonicate at 40 kHz and 30°C for 30 minutes, and then filter to collect the supernatant; centrifuge the supernatant at 45°C for 10 minutes, then store at 4°C to remove the condensed beeswax on the surface to obtain a propolis extract; mix 2 g of the propolis extract with 2 g of resveratrol and 2 g of eugenol to obtain a core material, and store at -30°C for later use;

[0063] C2. Dissolve 8 g of β-cyclodextrin in 20 mL of deionized water at room temperature, stir evenly, add 4 g of gum arabic, and homogenize in a machine for 30 minutes. Raise the system temperature to 100°C, continue stirring for 20 minutes, then add the core material, stir the system at 450 rpm for 1 hour, lower the system temperature to room temperature, ultrasonically treat for 20 minutes, and then continue heating to 100°C. After reacting for 12 hours, collect the system solution, spray dry it at a flow rate of 10 mL / min, a nozzle diameter of 1.5 mm, an inlet temperature of 135°C, and an outlet temperature of 85°C to obtain a long-acting antibacterial agent. Example

[0064] A method for raising high-quality Wagyu cattle comprises the following steps:

[0065] (1) Seed selection: The breeding base purchases imported basic cows, selects purebred Japanese black hair Wagyu embryos, takes two embryos and implants them into the bodies of imported basic cows, and carries out a gestation period of 9-10 months. Wagyu cattle weighing 150±5kg are selected for naturalization. They are first cleaned and disinfected in vitro, and shallow enema is performed under a sterile environment until the feces in the rectum are cleared to obtain clean-boweled Wagyu cattle; the local Mishima cattle breed produced in Mishima, Hagi City, Yamaguchi Prefecture is selected, and its fresh feces are collected to obtain wild Wagyu feces;

[0066] (2) Cowshed setup: Choose an area with a slope of 30°, good drainage, sheltered from the wind and facing the sun, quiet environment, convenient transportation, sufficient water and electricity, and low groundwater level. Set up the cowshed in an east-west direction and facing south. Prepare sunshade nets in summer and roller blinds in winter. Put down the sunshade nets in summer and roll up the roller blinds around to form a sweeping wind. Set up feed troughs, water troughs and air conditioners, set up underground drainage, and set up 24V low-voltage DC power supply lighting. The illumination of each area is 50Lux, with 16 hours of light and 8 hours of darkness per day.

[0067] (3) Daily feeding: Mix the extract of Cyperus multiflorus and the extract of Rosa roxburghii in equal proportions to obtain a composite plant extract, which is stored for future use; then prepare feed according to 80 parts of alfalfa, 1 part of the composite plant extract, 0.1 part of vitamin A, 0.1 part of vitamin D, 30 parts of corn, 10 parts of beetroot, 20 parts of soybean, and 10 parts of the feed additive prepared in Example 1 to obtain a customized feed for cows; prepare feed according to 100 parts of alfalfa, 1 part of the composite plant extract, 0.1 part of vitamin A, 0.1 part of vitamin D, 20 parts of corn, 5 parts of beetroot, 10 parts of soybean meal, and 10 parts of the feed additive prepared in Example 1 to obtain a customized feed for bulls; feed cows, bulls, and calves with the customized feed respectively, with cows and bulls fed twice a day, each time with 2.3% of their body weight, and free access to water; calves can eat and drink freely;

[0068] (4) Cowshed management: According to the 2.0m2 standard for cattle weighing less than 100kg 2 / head, 4.5m for 100-400kg 2 / head, 6.5m for over 500kg 2 The cattle are raised at a stocking density of 1 / head. Every morning, 45 minutes after feeding, the Wagyu cattle are taken for a walk in an open area. Bulls walk for 45 minutes, and cows walk for 60 minutes. The cowshed is disinfected and treated with a long-lasting antibacterial agent once a week. Deworming is carried out 2-3 times a year. Music is played in the cowshed for 2 hours every day. The Wagyu cattle are massaged with a brush every week. The Wagyu cattle are given a medicated bath by spraying every month. Example

[0069] The difference between this embodiment and embodiment 3 is that in step (3), the formula of the customized feed for cows is: 90 parts of alfalfa, 5 parts of compound plant extract, 0.2 parts of vitamin A, 0.6 parts of vitamin D, 45 parts of corn, 15 parts of beetroot, 25 parts of soybean, and 15 parts of the feed additive prepared in embodiment 2; the formula of the customized feed for bulls is: 120 parts of alfalfa, 5 parts of compound plant extract, 0.2 parts of vitamin A, 0.3 parts of vitamin D, 25 parts of corn, 8 parts of beetroot, 15 parts of soybean meal, and 15 parts of the feed additive prepared in embodiment 2. Example

[0070] The difference between this embodiment and Example 7 is that in step (3), the formula of the customized feed for cows is: 105 parts of alfalfa, 10 parts of compound plant extract, 0.5 parts of vitamin A, 0.5 parts of vitamin D, 60 parts of corn, 20 parts of beetroot, 30 parts of soybean, and 20 parts of the feed additive prepared in Example 3; the formula of the customized feed for bulls is: 150 parts of alfalfa, 10 parts of compound plant extract, 0.5 parts of vitamin A, 0.5 parts of vitamin D, 30 parts of corn, 10 parts of beetroot, 20 parts of soybean meal, and 20 parts of the feed additive prepared in Example 3. Example

[0071] The difference between this embodiment and Example 7 is that in step (3), the formula of the customized feed for cows is: 105 parts of alfalfa, 10 parts of compound plant extract, 0.5 parts of vitamin A, 0.5 parts of vitamin D, 60 parts of corn, 20 parts of beetroot, 30 parts of soybean, and 20 parts of the feed additive prepared in Example 4; the formula of the customized feed for bulls is: 150 parts of alfalfa, 10 parts of compound plant extract, 0.5 parts of vitamin A, 0.5 parts of vitamin D, 30 parts of corn, 10 parts of beetroot, 20 parts of soybean meal, and 20 parts of the feed additive prepared in Example 4. Example

[0072] The difference between this embodiment and Example 7 is that in step (3), the formula of the customized feed for cows is: 105 parts of alfalfa, 10 parts of compound plant extract, 0.5 parts of vitamin A, 0.5 parts of vitamin D, 60 parts of corn, 20 parts of beetroot, 30 parts of soybean, and 20 parts of the feed additive prepared in Example 5; the formula of the customized feed for bulls is: 150 parts of alfalfa, 10 parts of compound plant extract, 0.5 parts of vitamin A, 0.5 parts of vitamin D, 30 parts of corn, 10 parts of beetroot, 20 parts of soybean meal, and 20 parts of the feed additive prepared in Example 5.

[0073] Comparative Example 1

[0074] The difference between this comparative example and Example 7 is that the feed additive is quercetin, and the remaining steps and raw materials are the same as Example 7.

[0075] Comparative Example 2

[0076] The difference between this comparative example and Example 7 is that the feed additive is only tannic acid, and the remaining steps and raw materials are the same as Example 7.

[0077] Comparative Example 3

[0078] The difference between this comparative example and Example 7 is that the feed additive is only pea protein concentrate, and the remaining steps and raw materials are the same as Example 7.

[0079] Comparative Example 4

[0080] The difference between this comparative example and Example 7 is that the feed additive is only potato protein isolate, and the remaining steps and raw materials are the same as Example 7.

[0081] Comparative Example 5

[0082] The difference between this comparative example and Example 7 is that 1.5g of feed additive is 0.75g of potato protein isolate and 0.75g of pea protein concentrate, and the remaining steps and raw materials are the same as those in Example 7.

[0083] Comparative Example 6

[0084] The difference between this comparative example and Example 7 is that 70% ethanol solution is used instead of the long-acting antibacterial agent to disinfect the cowshed, the frequency remains the same, and the remaining steps and raw materials are synchronized with Example 7.

[0085] Comparative Example 7

[0086] The difference between this comparative example and Example 7 is that bulls and cows are not raised separately, and the feed formula is: 120 parts of alfalfa, 5 parts of compound plant extract, 0.2 parts of vitamin A, 0.3 parts of vitamin D, 25 parts of corn, 8 parts of beetroot, 15 parts of soybean meal, and 15 parts of the feed additive prepared in Example 1.

[0087] Comparative Example 8

[0088] The difference between this comparative example and Example 7 is that the selected Wagyu cattle were not subjected to naturalization treatment, and were raised individually for 2 weeks before being raised together.

[0089] Growth and development tests were conducted on the Wagyu cattle raised in Examples 7-11 and Comparative Examples 1-8. The Wagyu cattle were raised according to the ecological Wagyu farming methods described in Examples 7-11 and Comparative Examples 1-8. 100 healthy, disease-free Wagyu cattle aged 45-90 days were randomly selected and their growth was recorded. The results are shown in Table 1.

[0090]

[0091] The results in Table 1 show that the Wagyu cattle raised in Examples 7-11 had faster fattening rates and fewer illnesses. The results in the comparative examples show that encapsulating tannins with plant protein can increase cattle's feed intake and rumen protein content, thereby improving average daily weight gain. In addition to inhibiting bacteria, the long-acting antibacterial agent also reduces the likelihood of cattle becoming ill, and the fragrant environment it creates can also increase average daily weight gain. The results in Comparative Example 4 show that naturalization treatment can reduce the likelihood of cattle becoming ill.

[0092] The meat quality of the Wagyu cattle raised in Examples 7-11 and Comparative Examples 1-8 was tested. After the rearing period, five male and five female cows, each raised according to the ecological Wagyu farming methods of Examples 7-11 and Comparative Examples 1-8, were selected as test cattle for slaughter and evaluated according to Japanese Wagyu beef grading standards. The test cattle were weighed before slaughter, and 30 minutes after carcass cutting, the meat quality was assessed under incandescent lighting based on indicators such as marbling, physiological maturity, muscle color, and fat color.

[0093] The Wagyu cattle raised in this example had the richest marbling. Using customized feed can improve the quality of beef from female Wagyu cattle. Encapsulated tannic acid can increase the intramuscular fat content of beef, achieving a better meat quality grade.

[0094] Throughout the specification, references to terms such as "one embodiment," "example," or "specific example" indicate that the specific features, structures, materials, or characteristics described in conjunction with that embodiment or example are included in at least one embodiment or example of the present invention. In this specification, schematic representations of these terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

[0095] The above contents are merely examples and explanations of the present invention. Those skilled in the art may make various modifications or additions to the described specific embodiments or replace them in similar ways. As long as they do not deviate from the invention or exceed the scope defined by the claims, they should all fall within the scope of protection of the present invention.

Claims

1. A method for breeding high-quality Wagyu cattle, characterized in that: The following steps are involved: (1) Seed selection: The breeding base purchases imported foundation cows, selects purebred Japanese black-haired Wagyu, Japanese red-haired Wagyu, Japanese short-horned Wagyu, Japanese hornless Wagyu and their hybrids, and implants two Wagyu embryos into the bodies of imported foundation cows. After a gestation period of 9-10 months and a breeding period of 28-30 months, an operation cycle is completed. Among them, the imported foundation cows are eliminated after two pregnancies, and the Wagyu cattle are naturalized and bred during the breeding period; The specific methods of naturalization are: A1. Clean the selected Wagyu beef, disinfect it in vitro, and perform a shallow enema under a sterile environment until all feces in the rectum are removed, thereby obtaining a cleansed Wagyu beef. A2. Select the native Mishima cattle breed from Mishima, Hagi City, Yamaguchi Prefecture, and collect fresh feces to obtain wild Wagyu feces; A3. Add wild Wagyu feces to the drinking water of cleansed Wagyu cattle, raise the cleansed Wagyu cattle, provide them with food, and allow them to eat freely. After 1-2 weeks, naturalized Wagyu cattle are obtained; (2) Cowshed setup: Choose an area with a slope of 30°, good drainage, sheltered from the wind and facing the sun, quiet environment, convenient transportation, sufficient water and electricity, and low groundwater level. Set up the cowshed in an east-west direction and facing south. Prepare sunshade nets in summer and roller blinds in winter. Put down the sunshade nets in summer and roll up the roller blinds around to form a sweeping wind. Set up feed troughs, water troughs and air conditioners, set up underground drainage, and set up 24V low-voltage DC power supply lighting. The illumination of each area is 50Lux, with 16 hours of light and 8 hours of darkness per day. (3) Daily feeding: Cows, bulls, and calves were fed with customized feeds. Cows and bulls were fed twice a day, each feeding 2.0-2.5% of their body weight. Calves were fed ad libitum. The cattle were marketed when their weight reached 800±25kg. (4) Cowshed management: Every morning, after feeding for 30-60 minutes, the cattle are arranged to walk in an open area. Bulls walk for 45-60 minutes, and cows walk for 60-90 minutes. The cowshed is disinfected and treated with a long-lasting antibacterial agent once a week. Deworming is done 2-3 times a year. Music is played in the cowshed for 2 hours every day. The cattle are massaged with a brush every week. The cattle are given a medicated bath by spraying every month. The customized feed includes the following components: The customized feed for cows is 80-105 parts of pasture, 1-10 parts of compound plant extracts, 0.1-1 parts of compound vitamins, 30-60 parts of corn, 10-20 parts of beetroot, 20-30 parts of soybeans, and 10-20 parts of feed additives; The customized feed for bulls is 100-150 parts of forage, 1-10 parts of compound plant extracts, 0.1-1 parts of compound vitamins, 20-30 parts of corn, 5-10 parts of beetroot, 10-20 parts of soybean meal, and 10-20 parts of feed additives; The feed additive is a nanoparticle with plant protein as the wall material and polyphenols as the core material; The feed additive is prepared by the following steps: B1. Dispersing the plant protein in deionized water, placing it in a 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid buffer, and then adding polyphenols; heating at 80° C. for 30 minutes, cooling in a cold water bath, and then storing at 4° C. overnight to obtain a protein-polyphenol gel; B2. Dispersing the protein-polyphenol gel in 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid buffer for 5 minutes, degassing at 2200 rpm for 1 minute, and then homogenizing the protein dispersion three times to obtain a feed additive.

2. The method for breeding high-quality Wagyu cattle according to claim 1, characterized in that: In step A1, the weight of the selected Wagyu cattle is 150kg-200kg.

3. The method for breeding high-quality Wagyu cattle according to claim 1, characterized in that: The plant protein is at least one of pea protein concentrate and potato protein isolate.

4. The method for breeding high-quality Wagyu cattle according to claim 1, characterized in that: The polyphenolic substance is one of quercetin, tea polyphenols and tannic acid.

5. The method for breeding high-quality Wagyu cattle according to claim 1, characterized in that: The composite plant extract comprises a Cyperus multicephala extract and a Rosa roxburghii extract.

6. The method for breeding high-quality Wagyu cattle according to claim 1, characterized in that: In step (4), the long-acting antibacterial agent is prepared by the following steps: C1. Mixing ethanol and raw propolis, followed by ultrasonic treatment for 30 minutes, and then filtering to collect the supernatant; centrifuging the supernatant, and then storing it at 4°C to remove the condensed beeswax on the surface to obtain a propolis extract; Mixing propolis extract, resveratrol and eugenol in a mass ratio of 1:1:1 to obtain a core material, and storing the core material for later use; C2. Dissolve β-cyclodextrin in deionized water at room temperature, stir evenly, add gum arabic, and homogenize in a machine for 30 minutes. Raise the system temperature to 100°C, continue stirring for 20 minutes, then add the core material and stir for 1 hour. Lower the system temperature to room temperature, ultrasonicate for 20 minutes, and then continue heating to 100°C. After reacting for 12 hours, collect the system solution and spray dry it to obtain a long-acting antibacterial agent.

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