A whitening mask and a preparation method thereof

Abstract

The present application relates to the technical field of cosmetics, more particularly to a whitening mask, comprising a mask base solution and a mask non-woven fabric, wherein the mask base solution comprises at least 3-5 parts of jojoba oil, 0.2-0.3 parts of D-pantothenyl alcohol, 5-10 parts of a filtrate of a fermentation product of schizochytrium sp., 1-3 parts of dicaprylyl carbonate, 0.5-3 parts of a whitening composition, 2-3 parts of nicotinamide, 0.01-0.3 parts of tetrahydroxypropyl ethylenediamine, 0.01-0.2 parts of tocopheryl acetate, 5.7-15 parts of an auxiliary agent, and 60-80 parts of deionized water. The whitening composition is formulated to make the base solution system of the prepared whitening mask have excellent whitening effect, effectively inhibit the activity of tyrosinase, make the skin not easy to produce melanin, and achieve the effect of effectively whitening the skin.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, particularly to the field of IPC A61, and more specifically, to a whitening facial mask and its preparation method. Background Technology

[0002] Currently, skin darkening and dullness caused by melanin accumulation and deposition remain a challenge the beauty and skincare industry is tackling. While fair skin is still considered beautiful by many, daily life factors such as excessive ultraviolet radiation from sunlight, lack of sleep, and diet can all contribute to increased melanin production. With the rapid development of the medical aesthetics industry, various whitening treatments and products have emerged, among which whitening masks are the most affordable and convenient to use. Whitening masks are those that incorporate ingredients that promote melanin metabolism or inhibit tyrosinase activity into their essence.

[0003] CN106265419A discloses a whitening and moisturizing facial mask liquid and its preparation method. It uses sea cucumber intestine extract, aloe vera juice and egg white to make a compound. The components work synergistically to whiten and remove spots. However, heavy metal residues are easily generated in sea cucumber intestine extract, so the facial mask liquid contains heavy metals. Long-term use will cause damage to the human body.

[0004] Besides the issues mentioned above, the effects of existing whitening masks are also relatively limited. Some formulas use a single ingredient with only a whitening effect. However, human skin is quite complex, and the causes of melanin deposition are multifaceted. For example, inflammatory skin diseases or other causes can damage the skin barrier, leading to pigmentation at the damaged areas. Furthermore, while some whitening ingredients have a whitening effect during use, consumers often experience post-treatment hyperpigmentation (darkening) or even worsening of the skin after a period of use, especially after exposure to UV radiation or skin inflammation. Continuous stimulation weakens the skin barrier, potentially reducing the number of thiohydroxyl groups in the epidermis and promoting melanin synthesis, resulting in pigmentation. If this intolerance triggers inflammation, it can also irritate the skin, increasing melanin production.

[0005] To address the aforementioned problems, this invention provides a whitening facial mask that can simultaneously solve these issues. It not only blocks melanin production but also repairs the skin barrier and reduces inflammation, thus achieving whitening from a secondary perspective. Summary of the Invention

[0006] The first aspect of the present invention provides a whitening facial mask, comprising a mask base liquid and a mask nonwoven fabric. By weight, the raw materials of the mask base liquid include at least: 3-5 parts jojoba oil, 0.2-0.3 parts D-panthenol, 5-10 parts Bifida ferment lysate filtrate, 1-3 parts dioctyl carbonate, 0.5-3 parts whitening composition, 2-3 parts niacinamide, 0.01-0.3 parts tetrahydroxypropyl ethylenediamine, 0.01-0.2 parts tocopheryl acetate, 5.7-15 parts auxiliary agents, and 60-80 parts deionized water.

[0007] Preferably, the whitening composition comprises: 1.0-2.0 parts of mulberry bark extract, 0.5-2.0 parts of white peony root extract, 0.5-2.0 parts of hydrolyzed pearl solution, and 1.0-2.0 parts of marshmallow root extract.

[0008] Preferably, the whitening composition comprises: 1.5 parts mulberry bark extract, 1.0 part white peony root extract, 1.0 part hydrolyzed pearl solution, and 1.5 parts marshmallow root extract.

[0009] This application utilizes a whitening composition comprising 1.5 parts mulberry bark extract, 1.0 part white peony root extract, 1.0 part hydrolyzed pearl solution, and 1.5 parts marshmallow root extract. Through the synergistic effect of these ingredients, the whitening mask's skin-whitening efficacy is enhanced. The plausible reason is that the specific proportions of mulberry bark extract and hydrolyzed pearl solution work together to inhibit tyrosinase activity, reducing melanin production in the skin. Furthermore, the addition of specific proportions of white peony root extract and marshmallow root extract exerts anti-inflammatory effects, inhibiting pigmentation caused by skin inflammation or other irritants, thus enhancing the whitening effect. By controlling the specific components and proportions of this whitening composition, the effects on skin barrier repair and anti-inflammation are improved, effectively resisting ultraviolet rays while simultaneously making the product's ingredients more stable.

[0010] Preferably, the additive is at least one selected from emulsifiers, surfactants, thickeners, antioxidants, humectants, preservatives, and pH adjusters.

[0011] Preferably, the additives are emulsifiers, thickeners, preservatives, and humectants, and the mass ratio of emulsifiers, thickeners, preservatives, and humectants is (1.2-3):(0.5-2):(1-2.5):(3-8).

[0012] Preferably, the mass ratio of the emulsifier, thickener, preservative, and humectant is 1.8:0.6:2.05:3.62.

[0013] Preferably, the emulsifier is at least one selected from polysorbate-60, PEG-100 stearate, lecithin, glyceryl stearate, cetyl ether-20, polyoxyethylene lanolin, cetearyl glucoside, sucrose stearate, and polyoxyethylene lanolin.

[0014] Preferably, the emulsifier is a mixture of polysorbate-60, PEG-100 stearate, and cetearyl glucoside, wherein the mass ratio of polysorbate-60, PEG-100 stearate, and cetearyl glucoside is (0.3-0.6):(0.5-1.5):(0.1-0.5).

[0015] Preferably, the mass ratio of polysorbate-60, PEG-100 stearate, and cetearyl glucoside is 0.5:1:0.3.

[0016] Preferably, the thickener is at least one of carbomer, acrylate / C10-30 alkanol acrylate crosspolymer, xanthan gum, gelatin, carboxymethyl cellulose, polyacrylamide, and ammonium acryloyldimethyl taurate / VP copolymer.

[0017] Preferably, the thickener is a mixture of xanthan gum, acrylate / C10-30 alkanol acrylate crosspolymer, and polyacrylamide, wherein the mass ratio of xanthan gum, acrylate / C10-30 alkanol acrylate crosspolymer, and polyacrylamide is (0.1-0.3):(0.1-0.5):(0.05-0.2).

[0018] Preferably, the mass ratio of xanthan gum, acrylate / C10-30 alkanol acrylate crosslinker, and polyacrylamide is 0.2:0.3:0.1.

[0019] Preferably, the preservative is at least one selected from phenoxyethanol, benzyl alcohol, 1,2-pentanediol, octyl glycol, 1,2-hexanediol, p-hydroxyacetophenone, methylparaben, ethylhexylglycerin, glyceryl caprylate, and chlorphenesin.

[0020] Preferably, the preservative is 1,2-pentanediol, p-hydroxyacetophenone, octyl glycol, 1,2-hexanediol, and the mass ratio of 1,2-pentanediol, p-hydroxyacetophenone, octyl glycol, and 1,2-hexanediol is (1-1.5):(0.3-0.4):(0.1-0.3):(0.1-0.3).

[0021] Preferably, the mass ratio of 1,2-pentanediol, p-hydroxyacetophenone, octyl glycol, and 1,2-hexanediol is 1.2:0.35:0.25:0.25.

[0022] Preferably, the moisturizer is at least one selected from butylene glycol, sodium hyaluronate, glycerin, sodium lactate, propylene glycol, sorbitol, β-glucan, Tremella fuciformis extract, hydroxyethyl urea, vitamin B5, acetylated sodium hyaluronate, betaine, and trehalose.

[0023] Preferably, the moisturizer is a mixture of butylene glycol, sodium hyaluronate, Tremella fuciformis extract, and β-glucan, wherein the mass ratio of butylene glycol, sodium hyaluronate, Tremella fuciformis extract, and β-glucan is (3-3.5):(0.05-0.15):(0.01-0.03):(0.05-0.2).

[0024] Preferably, the mass ratio of butylene glycol, sodium hyaluronate, Tremella fuciformis extract, and β-glucan is 3.4:0.1:0.02:0.1.

[0025] A second aspect of the present invention provides a method for preparing the whitening facial mask as described above, characterized in that it includes at least the following steps:

[0026] S1 is used to prepare the mask base solution;

[0027] S2 Take 23-30g of mask base liquid into a container, fold the non-woven mask fabric and put it into the mask base liquid, and wait until the non-woven mask fabric is completely soaked.

[0028] Preferably, step S1 specifically comprises:

[0029] S11: Weigh out butanediol, sodium hyaluronate, tremella fruiting body extract, thickener, preservative and deionized water according to the weight fraction of raw materials, mix them, heat to 60-70℃, stir at 150-200r / min, stir evenly and completely dissolve.

[0030] S12: Mix jojoba oil, dioctyl carbonate, emulsifier, and tocopheryl acetate, heat to 70-80℃, stir at 150-200 r / min until homogeneous and completely dissolved;

[0031] S13: Add the mixture obtained in S12 to the mixture obtained in S11, homogenize for 5-10 minutes at a homogenization speed of 4000-5000 r / min, and then stop heating;

[0032] S14: After the mixture obtained in S13 is cooled to 42-48℃, keep the stirring speed at 150-200r / min, and add the following in sequence: Bifida ferment filtrate, D-panthenol, nicotinamide, β-glucan, mulberry bark extract, white peony root extract, hydrolyzed pearl solution, and marshmallow root extract. Adjust the pH of the system to 5.5-6.5 using tetrahydroxypropyl ethylenediamine, and continue homogenizing for 2-5 minutes at a speed of 4000-5000r / min. Cool to 35-40℃ to obtain the mask base solution.

[0033] Beneficial effects:

[0034] 1. This application provides a whitening facial mask. By formulating a whitening composition, the base liquid system of the prepared whitening facial mask has excellent whitening effect, can effectively inhibit the activity of tyrosinase, makes the skin less likely to produce melanin, and can achieve the effect of effectively whitening the skin.

[0035] 2. This application exerts anti-inflammatory effects by adding a specific ratio of Cynanchum atratum extract and Marshmallow root extract, inhibiting pigmentation caused by skin inflammation or other irritants, thereby achieving a whitening and enhancing effect.

[0036] 3. This application uses 1,2-pentanediol, p-hydroxyacetophenone, octyl glycol, and 1,2-hexanediol in a mass ratio of 1.2:0.35:0.25:0.25 as preservatives, which can replace traditional preservatives to prevent skin irritation, while inhibiting the growth of microorganisms in the mask base and improving product stability.

[0037] 4. This application uses polysorbate-60, PEG-100 stearate, and cetearyl glucoside in a mass ratio of 0.5:1:0.3 as emulsifiers to form a uniform and stable emulsion system between the oil and water-soluble components in the system, which is beneficial for each component to exert its efficacy stably.

[0038] 5. This application uses butylene glycol, sodium hyaluronate, Tremella fuciformis extract, and β-glucan in a mass ratio of 3.4:0.1:0.02:0.1 as a skin moisturizer. The ingredients are healthy and will not irritate the skin. By maintaining and absorbing skin moisture, it improves the moisturizing effect of the mask. Attached Figure Description

[0039] Figure 1 The image shows the sensory evaluation results of the whitening face mask prepared in Example 1.

[0040] Figure 2 This is a bar chart showing the results of live cell layer thickness testing of the whitening composition in Example 1.

[0041] Figure 3 This is a bar chart showing the LOR integrated optical density (IOD) value detection results of the whitening composition in Example 1.

[0042] Figure 4 A bar chart showing the results of tyrosinase inhibition rate testing for different skin whitening compositions. Detailed Implementation

[0043] Example 1

[0044] Example 1 provides a whitening facial mask, comprising a mask base liquid and a mask non-woven fabric. By weight, the raw materials of the mask base liquid include: 3.5 parts jojoba oil, 0.2 parts D-panthenol, 10 parts Bifida ferment lysate filtrate, 2 parts dioctyl carbonate, 0.75 parts whitening composition, 3 parts niacinamide, 0.18 parts tetrahydroxypropyl ethylenediamine, 0.1 parts tocopheryl acetate, 8.07 parts auxiliary agents, and 72.2 parts deionized water.

[0045] The whitening composition consists of: 1.5 parts mulberry bark extract (Cortex Mori), 1.0 parts Cynanchum ratum Bge extract, 1.0 parts hydrolyzed pearl solution, and 1.5 parts Althaea officinalis root extract (Althaea officinalis Linn.).

[0046] The mulberry bark extract was purchased from Shanghai Lishengsheng Special Enterprise Development Co., Ltd., product number LS-01382;

[0047] The extract of Cynanchum paniculatum was purchased from Shanghai Ruidean Biotechnology Co., Ltd., product number ANTI-INFLAM.

[0048] The Marshmallow root extract was purchased from IDBIOSAS, product number TitrExtractMarhmallow.

[0049] The additives are emulsifiers, thickeners, preservatives, and humectants, with a mass ratio of 1.8:0.6:2.05:3.62.

[0050] The emulsifier is a mixture of polysorbate-60, PEG-100 stearate, and cetearyl glucoside, with a mass ratio of 0.5:1:0.3.

[0051] Polysorbate-60 was purchased from Croda Chemicals (Shanghai) Co., Ltd., product number SP TWEEN 20MBAL-LQ-(SG);

[0052] The thickener is a mixture of xanthan gum, acrylate / C10-30 alkanol acrylate crosspolymer, and polyacrylamide, with a mass ratio of xanthan gum, acrylate / C10-30 alkanol acrylate crosspolymer, and polyacrylamide of 0.2:0.3:0.1.

[0053] Acrylic (ester) / C10-30 alkanol acrylate crosslinking polymer was purchased from Lubrizol Advanced Materials, product number [missing information]. ULTREZ 20POLYMER;

[0054] The polyacrylamide was purchased from SEPIEGEL (Shanghai) Specialty Chemicals Co., Ltd., product number SEPIEGEL 305;

[0055] The preservatives are 1,2-pentanediol, p-hydroxyacetophenone, octyl glycol, and 1,2-hexanediol, with a mass ratio of 1.2:0.35:0.25:0.25.

[0056] The moisturizer is a mixture of butylene glycol, sodium hyaluronate, Tremella fuciformis fruiting body extract, and β-glucan, with a mass ratio of 3.4:0.1:0.02:0.1.

[0057] The extract of Tremella fuciformis fruiting body was purchased from Shanghai Huiwen Biotechnology Co., Ltd., with the product code Tremella fuciformis heteropolysaccharide (WSK).

[0058] The preparation method of the whitening facial mask as described above includes the following steps:

[0059] S1 is used to prepare the mask base solution;

[0060] S2 Take 28g of mask base liquid into a container, fold the non-woven mask fabric and put it into the mask base liquid, and wait until the non-woven mask fabric is completely soaked.

[0061] The specific steps of S1 are as follows:

[0062] S11: Weigh out butanediol, sodium hyaluronate, tremella fruiting body extract, thickener, preservative and deionized water according to the weight fraction of raw materials, mix them, heat to 65℃, stir at 180r / min, stir evenly and completely dissolve.

[0063] S12: Mix jojoba oil, dioctyl carbonate, emulsifier, and tocopheryl acetate, heat to 75°C, stir at 180 r / min until homogeneous and completely dissolved;

[0064] S13: Add the mixture obtained in S12 to the mixture obtained in S11, homogenize for 6 minutes at a homogenization speed of 4500 r / min, and then stop heating;

[0065] S14: After the mixture obtained in S13 is cooled to 45℃, while maintaining the stirring speed, add the following in sequence: Bifida ferment filtrate, D-panthenol, nicotinamide, β-glucan, mulberry bark extract, white peony root extract, hydrolyzed pearl solution, and marshmallow root extract. Adjust the pH of the system to 5.5-6.5 using tetrahydroxypropyl ethylenediamine, and continue homogenizing for 3 minutes at a speed of 4500 r / min. Cool to 36℃ to obtain the mask base solution.

[0066] Example for comparison:

[0067] The specific implementation method of this control group is the same as that of Example 1, except that the raw material of the mask base liquid does not contain whitening composition.

[0068] Comparative Example 1:

[0069] The specific implementation method of this control group is the same as that of Example 1, except that the whitening composition consists of 1.0 part of Cynanchum paniculatum extract and 1.5 parts of Marshmallow extract.

[0070] Comparative Example 2:

[0071] The specific implementation method of this control group is the same as that of Example 1, except that the whitening composition consists of 1.5 parts of mulberry bark extract and 1.5 parts of marshmallow root extract.

[0072] Comparative Example 3:

[0073] The specific implementation method of this control group is the same as that of Example 1, except that the whitening composition consists of 1.5 parts of mulberry bark extract and 1.0 part of white peony extract.

[0074] Comparative Example 4:

[0075] The specific implementation method of this control group is the same as that of Example 1, except that the whitening composition consists of 1.5 parts of mulberry bark extract and 1.0 part of hydrolyzed pearl solution.

[0076] Performance testing:

[0077] I. Sensory evaluation of the face mask:

[0078] 1. Selection criteria:

[0079] (1) Healthy women / men aged 30-50.

[0080] (2) I have not used any products with similar effects in the week prior to the test, and I have not participated in any other similar product evaluation projects.

[0081] (3) Willing to sign the Informed Consent Form.

[0082] 2. Screening criteria:

[0083] (1) No laser or chemical peeling has been performed.

[0084] (2) No strenuous outdoor activities were conducted while experiencing the product.

[0085] (3) Do not suffer from skin diseases, psoriasis, severe eczema, etc., and do not have highly sensitive skin.

[0086] (4) No large-area birthmarks, scratches, vitiligo or other characteristics on the face that may affect the test.

[0087] (5) Has not received any cosmetic surgery, facial surgery or other skin treatments.

[0088] (6) No hormone drugs / immunosuppressants have been used in the past month.

[0089] (7) Not pregnant / preparing for pregnancy / breastfeeding.

[0090] 3. Testing Method:

[0091] Researchers evaluated the facial skin of test subjects, selecting 15 qualified participants. Without using any skincare products, participants cleansed their facial skin with warm water. After resting for 30 minutes in a temperature- and humidity-controlled room, they entered the sensory evaluation laboratory. They received the whitening mask product prepared in Example 1, applied the mask to their faces according to the instructions, and left it on for 15 minutes. After removing the mask, they massaged in any excess mask liquid, completed a questionnaire, and recorded the evaluation results in Table 1.

[0092] 4. Explanation of the survey questionnaire scoring criteria:

[0093] Scoring criteria: 1-9 points

[0094] Appearance: Indicates the product's appearance. 1 means dislike, 9 means like.

[0095] Adhesion: How well the mask adheres to the skin when used. 1 indicates difficulty in adhering and wrinkling, while 9 indicates easy application and a very good fit.

[0096] Absorbency: How quickly the mask essence is absorbed after application. 1 indicates poor absorption, and 9 indicates excellent absorption.

[0097] Breathability: How breathable the skin is when using the product. 1 indicates very stuffy, 9 indicates very refreshing.

[0098] Moisturizing effect: The softness of the skin after use. 1 represents very dry skin, and 9 represents very moisturizing skin.

[0099] Moisturizing level: How moisturized the skin feels after use. 1 represents very dry skin, and 9 represents very moisturized skin.

[0100] Overall liking level: This is based on personal preference. 1 represents strong dislike, and 9 represents strong liking.

[0101] Table 1 Evaluation Results

[0102]

[0103]

[0104] Figure 1 The image shows the sensory evaluation results of the whitening face mask prepared in Example 1.

[0105] II. Mask stability test:

[0106] (1) Cold resistance test:

[0107] The whitening facial mask liquids prepared in Example 1, Control Example, and Comparative Examples 1-4 were placed into six 5mL centrifuge tubes and placed in refrigerators at -5, -10, and -18℃ for 24h, 48h, 7 days, 14 days, and 30 days, respectively. After being removed and brought to room temperature, they were observed.

[0108] Test results: The mask liquid in the above test group did not show any oil-water separation. After being taken out and restored to room temperature, it could still be used normally, and the mask had good cold resistance.

[0109] (2) Heat resistance test:

[0110] The whitening facial mask liquids prepared in Example 1, Control Example, and Comparative Examples 1-4 were respectively placed into 6 5mL centrifuge tubes and placed in an oven at 45℃ for 24h, 48h, 7 days, 14 days, and 30 days before being taken out for observation.

[0111] Test results: Upon removal, it was observed that the liquid in the above test groups did not exhibit any oil-water separation and showed no significant change compared to before placement, indicating good heat resistance of the masks.

[0112] (3) Centrifugation test

[0113] The whitening facial mask liquids prepared in Example 1, Control Example, and Comparative Examples 1-4 were placed in six 5mL centrifuge tubes and centrifuged at 4500r / min, 6000r / min, 8000r / min, and 10000r / min for 30min respectively.

[0114] Test results: Upon inspection, the mask liquid in the above test groups showed no oil-water separation, remained usable after returning to room temperature, and showed no significant changes compared to before placement. The masks performed well after centrifugation.

[0115] (4) Ultraviolet irradiation test

[0116] The whitening facial mask liquids prepared in Example 1, Control Example, and Comparative Examples 1-4 were placed in six 5mL transparent centrifuge tubes and placed in an ultraviolet irradiation chamber for 2, 7, 14, and 30 days, respectively. The results were recorded in Table 2.

[0117] Table 2

[0118]

[0119] Experimental Results: Upon observation, the whitening mask liquids prepared in the control example, comparative example 1, and example 1 showed no color change. However, the whitening mask liquids prepared in comparative examples 2-4 all exhibited varying degrees of yellowing after one week of UV exposure, with the color change becoming more pronounced with increasing exposure time. This indicates that single extracts of *Cynanchum paniculatum* or *Haloxylon ammodendron* cannot completely prevent the effects of UV radiation on whitening ingredients. Example 1 demonstrates that the composition of 1.5 parts *Morus alba* root bark extract + 1 part hydrolyzed pearl extract + 1 part *Cynanchum paniculatum* + 1.5 parts *Haloxylon ammodendron* extract effectively resists UV radiation, effectively protecting the whitening ingredients and ensuring the activity and stability of the composition.

[0120] III. Patch Test

[0121] Thirty subjects were selected in the experiment, and their responses were recorded.

[0122] Experimental Procedure: Filter paper soaked in the mask solution prepared in Example 1 was placed in the spot tester. Normal wells treated with deionized water served as blank controls. Both the sample and the blank control were applied to the flexor side of the subject's forearm, and the skin was gently pressed evenly with the palm of the hand for 24 hours. After removing the spot tester, the skin reaction was observed after a 30-minute interval, once the pressure marks disappeared. Skin reactions were observed again 24 hours and 48 hours after removing the spot tester.

[0123] The results showed that none of the 30 subjects developed light red spots, erythema, edematous erythema, significant redness and swelling, infiltration or papules, or papules or vesicles, indicating that the whitening mask was not irritating to human skin.

[0124] IV. Whitening effect test:

[0125] Thirty healthy adult female volunteers were selected and randomly divided into 5 groups of 6 each. A 2cm x 2cm area on the inner forearm was used as the test site, with one side as the test area and the other as the control area. Whitening mask samples prepared in Example 1, the control example, and Comparative Examples 1-4 were collected from each group at a concentration of 2.0 mg ± 0.1 mg sample / cm². 2The product was applied evenly to the test area every evening for 3 consecutive months. During the trial period, no other cosmetics were used on the test area. The changes in skin melanin content before and after the application of the sample were measured using a skin melanin and hemoglobin analyzer to evaluate the comparison of skin melanin content (MI) before and after the application of the sample. The test results are shown in Table 3.

[0126] Table 3

[0127]

[0128] The results showed that after three months of using the whitening masks prepared in the control example, comparative examples 1-4, and example 1 of this invention, the skin melanin content decreased by 3.16%, 2.87%, 19.25%, 22.65%, 27.24%, and 39.25%, respectively. The combination of four raw materials added to this invention—mulberry bark extract, hydrolyzed pearl extract, white peony extract, and marshmallow extract—achieved a significantly better whitening effect. In other words, the raw material combination of this invention is the most reasonable, with a more prominent whitening and brightening effect, and there was no problem of facial darkening after three months of use.

[0129] V. Repair Barrier Test (1)

[0131] Test principle:

[0132] First, the 3D epidermal skin model was stimulated with the anionic surfactant sodium dodecyl sulfate (SLS). An in vitro skin injury model was constructed, and the repair effect of the sample was evaluated by detecting changes in tissue morphology and lobe rhinol content in the skin model after sample treatment.

[0133] Table 4 Summary of Results of Viable Cell Layer Thickness Detection

[0134]

[0135]

[0136] Note: When performing statistical analysis using the t-test method, significance compared to the BC group is indicated by #, with P-value < 0.01 indicated by ##; significance compared to the NC group is indicated by *, with P-value < 0.05 indicated by *, and P-value < 0.01 indicated by **.

[0137] Figure 2The bar chart shows the results of the live cell layer thickness test of the whitening composition in Example 1. The results show that, compared with the NC group, the live cell layer thickness of the group containing *Cynanchum paniculatum* and *Hypericum perforatum* extracts showed no significant change and the stratum corneum was loose. This demonstrates that it not only has no repairing effect on tissue morphology but may also cause slight damage. In contrast, the group containing the composition of *Morus alba* root bark, hydrolyzed pearl, *Cynanchum paniculatum*, and *Hypericum perforatum* extracts showed an increase in the number of live cells and a denser arrangement, resulting in a significant increase in live cell layer thickness, with an improvement rate of 48.39%. (2)

[0139] Table 5 Summary of LOR Integrated Optical Density (IOD) Values

[0140]

[0141] Note: When performing statistical analysis using the t-test method, significance compared with the BC group is indicated by #, with P-value < 0.05 indicated by # and P-value < 0.01 indicated by ##; significance compared with the NC group is indicated by *, with P-value < 0.05 indicated by * and P-value < 0.01 indicated by **.

[0142] Figure 3 The bar chart shows the LOR integrated optical density (IOD) values ​​of the whitening composition in Example 1. Compared with the NC group, the LOR content of the combination of mulberry bark, hydrolyzed pearl, white peony root, and marshmallow extract was significantly increased, with an increase rate of 623.45%; while the LOR content of the white peony root and marshmallow extract group was significantly decreased. Therefore, the white peony root and marshmallow extract did not have a good repairing effect on the damaged skin barrier.

[0143] VI. Whitening effect test of whitening composition:

[0144] Test principle: Raw materials with tyrosinase activity inhibitory effects can reduce the conversion of dopa to dopaquinone, thereby reducing absorbance. The inhibitory effect of the raw materials on tyrosinase activity is evaluated based on the change in absorbance.

[0145] Main reagents: Mushroom tyrosinase (Sigma), PBS (Gibco), L-DOPA (Sinopharm Reagents)

[0146] Main equipment: Biosafety cabinet (ESCO, A2-6S1), microplate reader (Tecan, Spark).

[0147] Experimental method: Prepare the sample into a test solution of the corresponding concentration, prepare the reaction system, mix well, and set up 3 replicates and 1 background control well for each concentration.

[0148] Inhibition rate calculation: Inhibition rate = ((AB) - (CD)) / (AB) × 100%

[0149] Test results:

[0150] Table 6. Analysis of In Vitro Tyrosinase Inhibition Test Results

[0151]

[0152]

[0153] Note: When performing statistical analysis using the t-test method, the significance of the sample group compared with the control group is indicated by *, P-value < 0.05 is indicated by *, and P-value < 0.01 is indicated by **.

[0154] Figure 4 The bar chart shows the results of tyrosinase inhibition rate tests on the different whitening compositions. The results indicate that hydrolyzed pearl, *Cynanchum paniculatum*, and *Marshmallow* affect the inhibition rate of tyrosinase activity by *Morus alba* root bark. The composition containing all of these ingredients showed the most significant inhibition rate of tyrosinase activity at 81.47%.

Claims

1. A whitening facial mask, characterized in that, The mask includes a mask base liquid and a mask nonwoven fabric. By weight, the raw materials of the mask base liquid include at least: 3-5 parts jojoba oil, 0.2-0.3 parts D-panthenol, 5-10 parts Bifida ferment lysate filtrate, 1-3 parts dioctyl carbonate, 0.5-3 parts whitening composition, 2-3 parts niacinamide, 0.01-0.3 parts tetrahydroxypropyl ethylenediamine, 0.01-0.2 parts tocopheryl acetate, 5.7-15 parts auxiliary agents, and 60-80 parts deionized water. The whitening composition comprises, by weight, 1.0-2.0 parts of mulberry bark extract, 0.5-2.0 parts of white peony root extract, 0.5-2.0 parts of hydrolyzed pearl solution, and 1.0-2.0 parts of marshmallow root extract; The additive is at least one of emulsifiers, surfactants, thickeners, antioxidants, humectants, preservatives, and pH adjusters; The emulsifier is a mixture of polysorbate-60, PEG-100 stearate, and cetearyl glucoside, with a mass ratio of (0.3-0.6):(0.5-1.5):(0.1-0.5).

2. The whitening facial mask according to claim 1, characterized in that, The thickener, by weight, is at least one of carbomer, acrylate / C10-30 alkanol acrylate crosspolymer, xanthan gum, gelatin, carboxymethyl cellulose, polyacrylamide, and ammonium acryloyl dimethyl taurate / VP copolymer.

3. The whitening facial mask according to claim 2, characterized in that, The preservative, by weight, is at least one of phenoxyethanol, benzyl alcohol, 1,2-pentanediol, octyl glycol, 1,2-hexanediol, p-hydroxyacetophenone, methylparaben, ethylhexylglycerin, glyceryl caprylate, and chlorphenesin.

4. The whitening facial mask according to claim 3, characterized in that, The moisturizer, by weight, is at least one of butylene glycol, sodium hyaluronate, glycerin, sodium lactate, propylene glycol, sorbitol, β-glucan, Tremella fuciformis extract, hydroxyethyl urea, vitamin B5, acetylated sodium hyaluronate, betaine, and trehalose.

5. The whitening facial mask according to claim 4, characterized in that, The moisturizer, by weight, is a mixture of butylene glycol, sodium hyaluronate, Tremella fuciformis extract, and β-glucan, wherein the mass ratio of butylene glycol, sodium hyaluronate, Tremella fuciformis extract, and β-glucan is (3-3.5):(0.05-0.15):(0.01-0.03):(0.05-0.2).

6. A method for preparing a whitening facial mask according to claim 5, characterized in that, At least the following steps are included: S1 is used to prepare the mask base solution; S2 Take 23-30g of mask base liquid into a container, fold the non-woven mask fabric and put it into the mask base liquid, and wait until the non-woven mask fabric is completely soaked.

7. The method for preparing the whitening facial mask according to claim 6, characterized in that, The specific steps of S1 are as follows: S11: Weigh out butanediol, sodium hyaluronate, tremella fruiting body extract, thickener, preservative and deionized water according to the weight fraction of raw materials, mix them, heat to 60-70℃, stir at 150-200r / min, stir evenly and completely dissolve. S12: Mix jojoba oil, dioctyl carbonate, emulsifier, and tocopheryl acetate, heat to 70-80℃, stir at 150-200 r / min until homogeneous and completely dissolved; S13: Add the mixture obtained in S12 to the mixture obtained in S11, homogenize for 5-10 minutes at a homogenization speed of 4000-5000 r / min, and then stop heating; S14: After the mixture obtained in S13 is cooled to 42-48℃, keep the stirring speed at 150-200r / min, and add the following in sequence: Bifida ferment filtrate, D-panthenol, nicotinamide, β-glucan, mulberry bark extract, white peony root extract, hydrolyzed pearl solution, and marshmallow root extract. Adjust the pH of the system to 5.5-6.5 using tetrahydroxypropyl ethylenediamine, and continue homogenizing for 2-5 minutes at a speed of 4000-5000r / min. Cool to 35-40℃ to obtain the mask base solution.

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