A multi-spectral digital light processing projection system and a digital light illumination regulation method

Abstract

The application provides a multispectral digital light processing projection system and a digital light illumination control method. The system comprises a multispectral digital light beam coupling module, a plurality of monochromatic light beam generating units for generating monochromatic digital light beams of different wavelengths, a light beam composite unit for integrating the optical paths of the monochromatic digital light beams to finally combine the light beams into a multispectral digital light beam, a multispectral digital light beam generating module comprising a microscope objective, the multispectral digital light beam being focused by the microscope objective to generate a multispectral digital structured illumination light field, and a multispectral digital light beam detection module comprising an image capturing device for detecting the scattered light of the multispectral digital structured illumination light field to monitor the multispectral digital structured illumination light field in real time. According to the multispectral digital light processing projection system and the digital light illumination control method provided by the application, the excitation light spectrum and the illumination light field digital processing can be simultaneously controlled.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of optical illumination, in particular to a multi-spectral digital light processing projection system and a digital light illumination control method. BACKGROUND

[0002] Light field illumination control refers to the selection and control of the spectrum of the light field illumination and the control of the spatial intensity distribution of the illumination light field, and is one of the research hotspots in the field of optical technology.

[0003] Using digital light processing (DLP) technology, by rapidly patterning the illumination light field, is one of the main ways to realize the control of the illumination light field. The main device is a digital micromirror device (DMD), by controlling the deflection direction of each individual pixel on the chip, the spatial distribution of the incident light can be controlled, and then the graphical control of the illumination light field can be realized. However, the traditional single-wavelength DLP technology, due to the use of only a single specific wavelength light source, can only realize the control of the light field at a single wavelength, and cannot simultaneously realize the control of the illumination spectrum and the illumination spatial distribution. Patent 202110521360.6 A multi-color light illumination three-dimensional particle imaging system and method, by changing the laser light source of different wavelengths to realize the spectral control of the light field illumination, but does not control the spatial distribution of the illumination light field; Patent 202210080472.7 A multi-color structured light illumination super-resolution microscopic imaging method and device based on DMD, by controlling the different angles of the galvanometer swing to realize the time selection or cutoff of each wavelength laser, the structured light illumination light field at different wavelengths can be realized, but it cannot completely realize the simultaneous control of the illumination wavelength and the illumination light field distribution, and the application scene is relatively single.

[0004] Therefore, there is a need for a light field illumination system and control method that can flexibly control the illumination spectrum and the spatial distribution of the illumination light field simultaneously. SUMMARY

[0005] The embodiments of the present application provide a multi-spectral digital light processing projection system and a digital light illumination control method to at least solve one of the problems in the related art. To achieve this purpose, the present application is realized by the following technical scheme.

[0006] In one aspect, the present application provides a multi-spectrum structured light illumination system, comprising: a multi-spectrum digital light beam coupling module, comprising: a plurality of monochromatic light beam generating units for generating monochromatic digital light beams of different wavelengths; a light beam combining unit for integrating the light paths of the monochromatic digital light beams emitted by each of the monochromatic light beam generating units, and finally combining the light beams into a multi-spectrum digital light beam; a multi-spectrum digital light beam generating module comprising a microscope objective, the multi-spectrum digital light beam being focused by the microscope objective to generate a multi-spectrum digital structured illumination light field; and a multi-spectrum digital light beam detection module comprising an image capture device, the scattered light of the multi-spectrum digital structured illumination light field being collected by the microscope objective and detected by the image capture device to monitor the multi-spectrum digital structured illumination light field in real time.

[0007] Further, each of the monochromatic light beam generating units comprises: a monochromatic digital light processing projector for generating a monochromatic digital LED light beam by optical projection; and a collimating beam expander for collimating and expanding the monochromatic digital LED light beam.

[0008] Alternatively, each of the monochromatic light beam generating units comprises: a single-wavelength laser and a spatial light modulator matched with each other for generating a single-wavelength structured laser light beam by optical projection; and a collimating beam expander for collimating and expanding the single-wavelength structured laser light beam.

[0009] Alternatively, an LED light source and a spatial light modulator matched with each other for generating a monochromatic digital structured LED light beam by optical holography; and a collimating beam expander for collimating and expanding the monochromatic digital structured LED light beam.

[0010] Further, the light beam combining unit comprises N first dichroic mirrors, N / 2 second dichroic mirrors, N / 4 third dichroic mirrors, and so on, until the last dichroic mirror is one, wherein each of the first dichroic mirrors is used to transmit and reflect the light beams emitted by two monochromatic light beam generating units and then jointly incident on a second dichroic mirror; each of the second dichroic mirrors is used to transmit and reflect the light beams emitted by two first dichroic mirrors and then jointly incident on a third dichroic mirror, and so on, until the last dichroic mirror emits the multi-spectrum digital light beam.

[0011] Further, the multi-spectrum digital light processing projection system further comprises a control unit for controlling each of the monochromatic light beam generating units to realize any one of the following modes: monochromatic specified illumination light field illumination, monochromatic different illumination light field time sequence illumination, multi-spectrum specified illumination light field illumination, and multi-spectrum different illumination light field time sequence illumination.

[0012] Further, the multispectral digitalized light beam generating module further comprises a plano-convex lens, a half-transmission half-reflection mirror and a sleeve tube mirror. The multispectral digitalized light beam generated by the multispectral digitalized light beam coupling module passes through the plano-convex lens, is transmitted to the half-transmission half-reflection mirror, and then is transmitted to the microscope objective lens through the sleeve tube mirror to generate the multispectral digitalized structured illumination light field.

[0013] Further, the scattered light of the multispectral digitalized structured illumination light field is collected by the microscope objective lens, reflected by the half-transmission half-reflection mirror, and then imaged on the image capturing device.

[0014] In another aspect, the present application provides a digital light illumination control method based on the multispectral digital light processing projection system. The method comprises the following steps: a plurality of monochromatic light beam generating units generate monochromatic digitalized light beams with different wavelengths, respectively. After light path integration, the monochromatic digitalized light beams are finally combined into a multispectral digitalized light beam. The multispectral digitalized light beam is focused by a microscope objective lens to generate a multispectral digitalized structured illumination light field. The scattered light of the multispectral digitalized structured illumination light field is collected by the microscope objective lens and detected by an image capturing device to realize real-time monitoring of the multispectral digitalized structured illumination light field. By controlling each monochromatic light beam generating unit, any one of the following modes can be realized: monochromatic specified illumination light field illumination, monochromatic different illumination light field time sequence illumination, multispectral specified illumination light field illumination, and multispectral different illumination light field time sequence illumination.

[0015] The present application has the following advantages:

[0016] (1) Compared with the traditional monochromatic digital light processing technology, the multispectral digital light processing projection system provided by the present application comprises a plurality of monochromatic light beam generating units with different wavelengths, which can meet the demand of illumination light field for different illumination light wavelengths and realize multispectral illumination.

[0017] (2) By configuring a monochromatic digital light processing projector for each monochromatic light beam generating unit, or by cooperating a single-wavelength laser or LED light source with a spatial light modulator, the spatial distribution of the illumination light field can be controlled to provide a digitalized illumination light field.

[0018] (3) According to the multispectral digital light processing projection system and the digital light illumination control method provided by the present application, the illumination light wavelength and the illumination light field distribution can be simultaneously controlled.

[0019] (4) The multispectral digital light processing projection system provided by the present application has more flexibility in the illumination light field representation. By controlling each monochromatic light beam generating unit respectively, the illumination of monochromatic specific illumination light field, the time sequence illumination of monochromatic different illumination light field, the illumination of multispectral specific illumination light field, and the time sequence illumination of multispectral different illumination light field, and other light field illumination modes can be realized. BRIEF DESCRIPTION OF DRAWINGS

[0020] The accompanying drawings, which form a part of the present application, are intended to provide further understanding of the present application, and are used to explain the illustrative embodiments of the present application and their descriptions, and do not constitute improper limitations on the present application.

[0021] Figure 1 The accompanying drawings, which form a part of the present application, are intended to provide further understanding of the present application, and are used to explain the illustrative embodiments of the present application and their descriptions, and do not constitute improper limitations on the present application.

[0022] Figure 2 The accompanying drawings, which form a part of the present application, are intended to provide further understanding of the present application, and are used to explain the illustrative embodiments of the present application and their descriptions, and do not constitute improper limitations on the present application.

[0023] Reference signs:

[0024] 001 - multispectral digital light beam coupling module; 002 - multispectral digital light beam generating module; 003 - multispectral digital light beam detecting module; 1 - first monochromatic digital light processing projector; 2 - first collimating beam expander; 3 - first short-pass dichroic mirror; 4 - second collimating beam expander; 5 - second monochromatic digital light processing projector; 6 - first long-pass dichroic mirror; 7 - second short-pass dichroic mirror; 8 - third collimating beam expander; 9 - third monochromatic digital light processing projector; 10 - fourth collimating beam expander; 11 - fourth monochromatic digital light processing projector; 12 - plano-convex lens; 13 - half-transmission half-reflection mirror; 14 - camera; 15 - sleeve tube mirror; 16 - microscope objective lens; 17 - sample cell; 18 - three-dimensional displacement stage. DETAILED DESCRIPTION

[0025] In order to make the purpose, technical solutions and advantages of the embodiments of the present application more clear, the embodiments of the present application will be described in detail below with reference to the accompanying drawings. However, those skilled in the art can understand that in the embodiments of the present application, many technical details are proposed in order to make the readers better understand the present application. However, even without these technical details and various changes and modifications based on the following embodiments, the technical solutions claimed by the present application can be realized. The division of the following embodiments is for the convenience of description, and should not constitute any limitation on the specific implementation of the present application, and the embodiments can be combined and referred to each other without contradiction.

[0026] The present application provides a multispectral digital light processing projection system. Figure 1A structural schematic diagram of a multispectral digital light processing projection system according to an embodiment of the present application, Figure 2 A light path schematic diagram of a multispectral digital light processing projection system according to an embodiment of the present application. As shown in the figure, the multispectral digital light processing projection system comprises a multispectral digital light beam coupling module 001, a multispectral digital light beam generation module 002, and a multispectral digital light beam detection module 003. Figure 1

[0027] In this embodiment, the multispectral digital light beam coupling module 001 comprises a plurality of monochromatic light beam generation units for generating monochromatic digital light beams of different wavelengths, and a light beam combination unit for integrating the light paths of the monochromatic digital light beams emitted by the monochromatic light beam generation units, such as guiding or deflecting the light path propagation direction, and finally combining the beams into a multispectral digital light beam. In this embodiment, four monochromatic light beam generation units are taken as an example to introduce the design idea of the present application, and the monochromatic light beam generation units can be adjusted as needed in actual application.

[0028] In this embodiment, the monochromatic light beam generation unit comprises a monochromatic digital light processing projector for generating a monochromatic digital LED light beam through optical projection, and a collimating beam expander for collimating and expanding the monochromatic digital LED light beam. The process of generating a digital LED light beam by the monochromatic digital light processing projector is as follows: the light beam of a light source is irradiated on a DMD after passing through an eye lens, and the light beam reflected on the DMD generates a patterned digital light by controlling the DMD. The light intensity, phase, and direction of the output light can be changed by directly controlling the DMD chip. Figure 2 As shown in the figure, the digital LED light beam generated by the first monochromatic digital light processing projector 1 is collimated and expanded by the first collimating beam expander 2; similarly, the digital LED light beam generated by the second monochromatic digital light processing projector 5 is collimated and expanded by the second collimating beam expander 4; the digital LED light beam generated by the third monochromatic digital light processing projector 9 is collimated and expanded by the third collimating beam expander 8; and the digital LED light beam generated by the fourth monochromatic digital light processing projector 11 is collimated and expanded by the fourth collimating beam expander 10.

[0029] In this embodiment, it is assumed that the wavelength of the first monochromatic digital light processing projector > the wavelength of the second monochromatic digital light processing projector > the wavelength of the third monochromatic digital light processing projector > the wavelength of the fourth monochromatic digital light processing projector.

[0030] ​In an optional embodiment, the monochromatic light beam generating unit comprises a single-wavelength laser and a spatial light modulator matched with each other, for generating a single-wavelength structured laser light beam by optical projection; and a collimating expander for collimating and expanding the single-wavelength structured laser light beam. The working process of generating the structured laser light beam is as follows: the light source is irradiated on the spatial light modulator SLM, and the reflected light can obtain the desired structured light beam by program control of the SLM. The SLM is controlled by computer to change the properties of liquid crystal molecules to change the intensity, phase, direction, etc. of the input light beam. The built-in light source of the laser can be a solid-state laser or a femtosecond laser.

[0031] In another optional embodiment, the monochromatic light beam generating unit comprises an LED light source and a spatial light modulator matched with each other, for generating a monochromatic digital structured LED light beam by optical holographic method; and a collimating expander for collimating and expanding the monochromatic digital structured LED light beam. The working process of generating the digital structured LED light beam is as follows: the LED light source is irradiated on the spatial light modulator SLM, and the reflected light can obtain the desired digital structured light beam by program control of the SLM. The SLM is controlled by computer to change the properties of liquid crystal molecules to change the intensity, phase, direction, etc. of the input light beam.

[0032] In the embodiment, the light beam combining unit comprises N first dichroic mirrors, N / 2 second dichroic mirrors, N / 4 third dichroic mirrors, and so on, until the last dichroic mirror is 1. Each first dichroic mirror is used to transmit and reflect the two light beams from two monochromatic light beam generating units and then jointly incident on a second dichroic mirror; each second dichroic mirror is used to transmit and reflect the two light beams from two first dichroic mirrors and then jointly incident on a third dichroic mirror, and so on, until the last dichroic mirror emits a multi-spectrum digital light beam. For example, Figure 2As shown in the figure, the first short-pass dichroic mirror 3 and the second short-pass dichroic mirror 7 are both first-order dichroic mirrors, and the first long-pass dichroic mirror 6 is a second-order dichroic mirror. The digital LED light beam emitted by the first monochromatic light beam generating unit is transmitted on the front surface of the first short-pass dichroic mirror 3, and the digital LED light beam emitted by the second monochromatic light beam generating unit is reflected on the rear surface of the first short-pass dichroic mirror 3; then, the transmitted light beam and the reflected light beam of the first short-pass dichroic mirror 3 are incident on the rear surface of the first long-pass dichroic mirror 6 and are reflected. The digital LED light beam emitted by the third monochromatic light beam generating unit is transmitted on the front surface of the second short-pass dichroic mirror 7, and the digital LED light beam emitted by the fourth monochromatic light beam generating unit is reflected on the rear surface of the second short-pass dichroic mirror 7; then, the transmitted light beam and the reflected light beam of the second short-pass dichroic mirror 7 are incident on the front surface of the first long-pass dichroic mirror 6 and are transmitted. The transmitted light beam and the reflected light beam of the first long-pass dichroic mirror 6 are combined into a multi-spectral digital light beam.

[0033] As shown in the figure, Figure 2 The multi-spectral digital light beam generating module 002 includes a plano-convex lens 12, a half-transmission half-reflection mirror 13, a sleeve tube mirror 15, and a microscope objective 16. The multi-spectral digital light beam emitted by the multi-spectral digital light beam coupling module 001 passes through the plano-convex lens 12 to condense light and optimize the optical path. Then, the transmitted light beam is incident on the rear surface of the half-transmission half-reflection mirror 13 and is transmitted. The transmitted light beam passes through the sleeve tube mirror 15 to correct aberration, is incident on the microscope objective 16, and is focused on the focal plane of the microscope objective 16 to generate a multi-spectral digital structured illumination light field. Preferably, the microscope objective 16 is a wide-band achromatic lens to ensure that different wavelength spectra converge on the same focal plane as much as possible.

[0034] In this embodiment, the multi-spectral digital light processing projection system further includes a control unit for controlling each monochromatic light beam generating unit respectively, which can be independently turned on and off. The projection pattern generated by each monochromatic light beam generating unit can be independently and freely generated, so that any one of the monochromatic specified illumination light field illumination, the monochromatic different illumination light field time sequence illumination, the multi-spectral specified illumination light field illumination, and the multi-spectral different illumination light field time sequence illumination modes can be realized, so that the multi-spectral digital light processing projection system has more flexibility in the illumination light field performance mode.

[0035] As shown in the figure, Figure 2 The multi-spectral digital light processing projection system further includes a sample cell 17 placed on a high-precision three-dimensional displacement stage 18, wherein the sample contained in the sample cell 17 is located near the focal plane of the microscope objective 16 after height adjustment.

[0036] As shown in the figure, Figure 2As shown, the multi-spectral digitalized light beam detection module 003 comprises an image capturing device, such as a camera 14. The scattered light of the multi-spectral digitalized structured illumination light field is collected by a microscope objective 16 and detected by the camera 14 to monitor the multi-spectral digitalized structured illumination light field in real time.

[0037] In another aspect, the present application provides a digital light illumination control method based on the multi-spectral digital light processing projection system. The method comprises: a plurality of monochromatic light beam generating units respectively generating monochromatic digitalized light beams of different wavelengths, and after light path integration, the monochromatic digitalized light beams are finally combined into a multi-spectral digitalized light beam; the multi-spectral digitalized light beam after the beam combination is focused by a microscope objective to generate a multi-spectral digitalized structured illumination light field; the scattered light of the multi-spectral digitalized structured illumination light field is collected by a microscope objective and detected by an image capturing device to monitor the multi-spectral digitalized structured illumination light field in real time; wherein by respectively controlling each monochromatic light beam generating unit, any one of the following modes is realized: monochromatic specified illumination light field illumination, monochromatic different illumination light field time sequence illumination, multi-spectral specified illumination light field illumination, and multi-spectral different illumination light field time sequence illumination.

[0038] The multi-spectral digital light processing projection system and the digital light illumination control method provided by the present application can realize the simultaneous control of the illumination light wavelength and the illumination light field distribution.

[0039] Although the embodiments of the present application have been shown and described, it is to be understood that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present application, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A multispectral digital light processing projection system for digital light illumination regulation, which realizes simultaneous regulation of illumination spectrum and spatial distribution of illumination light field, characterized in that: a multispectral digital light beam coupling module: a plurality of monochromatic light beam generating units generate monochromatic digital light beams of different wavelengths; a light beam combining unit integrates the light paths of the monochromatic digital light beams emitted by each monochromatic light beam generating unit, and finally combines them into a multispectral digital light beam; the light beam combining unit includes a first short-pass dichroic mirror and a second short-pass dichroic mirror, both of which are first-order dichroic mirrors, and a first long-pass dichroic mirror, which is a second-order dichroic mirror; the digital LED light beam emitted by the first monochromatic light beam generating unit is transmitted on the front surface of the first short-pass dichroic mirror, and the digital LED light beam emitted by the second monochromatic light beam generating unit is reflected on the rear surface of the first short-pass dichroic mirror; the transmitted and reflected light beams of the first short-pass dichroic mirror are incident on the rear surface of the first long-pass dichroic mirror and are reflected; the digital LED light beam emitted by the third monochromatic light beam generating unit is transmitted on the front surface of the second short-pass dichroic mirror, and the digital LED light beam emitted by the fourth monochromatic light beam generating unit is reflected on the rear surface of the second short-pass dichroic mirror; the transmitted and reflected light beams of the second short-pass dichroic mirror are incident on the front surface of the first long-pass dichroic mirror and are transmitted; the transmitted and reflected light beams of the first long-pass dichroic mirror are combined into a multispectral digital light beam; each monochromatic light beam generating unit includes a collimating expander for collimating and expanding the monochromatic digital LED light beam; a multispectral digital light beam generating module: the multispectral digital light beam is focused by a microscopic objective to generate a multispectral digital structured illumination light field; after the multispectral digital light beam generated by the multispectral digital light beam coupling module passes through a plano-convex lens, it is transmitted by a half-transmission half-reflection mirror, and the transmitted light beam is incident on the microscopic objective through a sleeve tube mirror to be focused to generate the multispectral digital structured illumination light field; a multispectral digital light beam detection module, which includes an image capturing device, the scattered light of the multispectral digital structured illumination light field is collected by the microscopic objective and detected by the image capturing device to monitor the multispectral digital structured illumination light field in real time; each monochromatic light beam generating unit includes a monochromatic digital light processing projector for generating a monochromatic digital LED light beam by optical projection; and a control unit for controlling each monochromatic light beam generating unit to realize any one of the following modes: monochromatic specified illumination light field illumination, monochromatic different illumination light field time sequence illumination, multispectral specified illumination light field illumination, and multispectral different illumination light field time sequence illumination; the multispectral digital light beam generating module further includes a half-transmission half-reflection mirror, a plano-convex lens, and a sleeve tube mirror, and the multispectral digital light beam generated by the multispectral digital light beam coupling module passes through the plano-convex lens, is incident on the half-transmission half-reflection mirror, and is transmitted, and the transmitted light beam is incident on the microscopic objective through the sleeve tube mirror to be focused to generate the multispectral digital structured illumination light field. ​ ​ ​ ​ 2. The multi-spectral digital light processing projection system of claim 1, wherein, The scattered light of the multi-spectrum digital structured illumination light field is collected by the objective lens, passes through the sleeve lens, is reflected by the half-mirror, and is imaged on the image capture device.

3. A method of digital light regulation based on the multispectral digital light processing projection system of claim 1, characterized in that, The application relates to a multi-spectrum digital structured illumination light field imaging device. A plurality of monochromatic light beam generating units generate monochromatic digital light beams of different wavelengths, and the monochromatic digital light beams are finally combined into a multi-spectrum digital light beam through light path integration, so that multi-spectrum illumination is realized. The multi-spectrum digital light beam after combination is focused by an objective lens to generate a multi-spectrum digital structured illumination light field. The scattered light of the multi-spectrum digital structured illumination light field is collected by the objective lens and detected by an image capture device, so that the multi-spectrum digital structured illumination light field is monitored in real time. Any one of the following modes can be realized by controlling each monochromatic light beam generating unit: monochromatic specified illumination light field illumination, monochromatic different illumination light field time sequence illumination, multi-spectrum specified illumination light field illumination, and multi-spectrum different illumination light field time sequence illumination.

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