An antioxidant repair composition and method of making and using same

Abstract

The present application relates to the field of cosmetics, in particular to an antioxidant repair composition and its preparation method and application. The antioxidant repair composition prepared by the present application comprises the following components by weight: 1-5 parts of bamboo shoot extract, 1-5 parts of lycopodium extract and 1-5 parts of sheep ear chrysopsis extract. After compounding bamboo shoot, lycopodium and sheep ear chrysopsis, the synergistic effect can improve the active substance content of the composition, significantly improve the antioxidant effect of the composition, and further match the moxa tablet to promote penetration and absorption, significantly improve the repair effect, and can be applied in the field of cosmetics.

Description

Technical Field

[0001] This invention relates to the field of cosmetics, and more particularly to an antioxidant repair composition, its preparation method, and its application. Background Technology

[0002] Currently, plant extracts are increasingly widely used in cosmetics. While *Lycopodium clavatum* extract is clinically used to treat rheumatoid arthritis, cervical spondylosis, acute and chronic soft tissue injuries, and herpes zoster, its application in the daily chemical industry is limited. *Hemerocallis fulva* root is commonly used in traditional medicine in the Dong ethnic region of Guangxi to be infused in alcohol for anti-inflammatory, swelling-reducing, and analgesic purposes, treating joint pain with good efficacy. However, the material basis and mechanism of its anti-inflammatory and analgesic effects are still unclear, resulting in limited application in the daily chemical industry. Although *Fungi fungus* extract has some applications in the cosmetic industry, there are numerous extraction methods, including water extraction, alcohol extraction, enzyme extraction, ultrasonic extraction, supercritical carbon dioxide extraction, and extraction with other organic solvents. While organic solvent extraction can selectively yield some active components from bamboo fungus, the presence of organic solvents limits the application of the resulting bamboo fungus extract in the cosmetics industry. Ultrasonic extraction, although it can reduce extraction time and improve efficiency to some extent, suffers from high equipment costs and requires post-extraction treatment to obtain a clear extract, making it unsuitable for industrial production. Supercritical fluid extraction also faces limitations due to its high industrial production costs. Bioenzyme extraction offers a relatively low-cost and milder method, but its use requires specific pH levels and high-temperature inactivation, making the process complex and unsuitable for industrial applications. Although single-method water extraction can yield a satisfactory bamboo fungus extract, the high protein and amino acid content in bamboo fungus results in a dark and turbid extract, failing to meet the standards for cosmetic applications. Summary of the Invention

[0003] The purpose of this invention is to overcome the shortcomings of the existing technology and provide an antioxidant repair composition. The combination of bamboo fungus extract, lycopodium extract and chamomile extract has a synergistic effect, which can increase the content of active substances in the composition and significantly improve the antioxidant effect. When combined with artemisia tablets, the repair effect can be significantly improved. This composition can be applied in the cosmetic field.

[0004] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0005] In a first aspect, the present invention provides an antioxidant repair composition comprising the following components in parts by weight: 1-5 parts of bamboo fungus extract, 1-5 parts of lycopodium extract, and 1-5 parts of chamomile extract.

[0006] Bamboo fungus is a rare and precious fungus ingredient commonly used in medicinal cuisine. Its consumption in my country dates back to 618 AD. Bamboo fungus has effects such as lowering blood lipids, anti-oxidation, enhancing immunity, protecting the liver, and resisting radiation. It can also relieve pain, replenish qi, and lower blood pressure, offering health benefits and adjunctive treatment for hypertension, neurasthenia, and gastrointestinal diseases. Bamboo fungus is an excellent source of plant protein and nutrients, containing abundant volatile oils, amino acids, polysaccharides, and terpenoids. In the daily chemical industry, its applications focus on the antioxidant, anti-aging, and moisturizing effects of bamboo fungus polysaccharides and polypeptides.

[0007] Lycopodium is rich in alkaloids and triterpenoids, and also contains small amounts of anthraquinones and volatile oils. Modern pharmacological studies have shown that it has anti-inflammatory, analgesic, antibacterial, and acetylcholinesterase-inhibiting effects. Clinically, it is often used to treat rheumatoid arthritis, cervical spondylosis, acute and chronic soft tissue injuries, and herpes zoster.

[0008] *Hemerocallis fulva* mainly contains sesquiterpenes, triterpenes, sterols, flavonoids, and aromatic compounds, with triterpenes and sterols being the most abundant. In the Dong ethnic minority region of Guangxi, *Hemerocallis fulva* root is commonly used to make an alcoholic beverage for anti-inflammatory, swelling-reducing, and analgesic purposes, treating joint pain with good efficacy. However, the material basis and mechanism of its anti-inflammatory and analgesic effects are still unclear.

[0009] The weight proportions of bamboo fungus extract, lycopodium extract, and chamomile extract within the scope defined by this invention have good antioxidant and repair effects. The absence of any one of them or exceeding the scope of this invention will lead to a decrease in antioxidant and repair effects.

[0010] Preferably, in the above-mentioned antioxidant repair composition, the bamboo fungus extract is a water-extracted and ethanol-precipitated or ethanol-extracted extract; the lycopodium extract is an ethanol-extracted extract; and the chamaedorea extract is an ethanol-extracted extract.

[0011] More preferably, the bamboo fungus extract is a water-extracted and alcohol-precipitated extract.

[0012] The water extraction and alcohol precipitation method can meet the requirements for the clarity of raw materials in cosmetics, retain the active ingredients to the maximum extent, and is easy to implement in industrial production. It has the advantages of being green, environmentally friendly, and low-cost.

[0013] More preferably, the above-mentioned antioxidant repair composition includes the following components in parts by weight: 1 part of bamboo fungus extract, 1 part of lycopodium extract, and 1 part of chamaedorea extract.

[0014] Bamboo fungus extract, lycopodium extract, and chamomile extract, at specific weight proportions according to the present invention, exhibited the lowest half-maximal scavenging rate and the best antioxidant effect; the zebrafish embryo tail fin repair promotion rate reached as high as 23%, which was 8%-18% higher than the control group.

[0015] Preferably, the above-mentioned antioxidant repair composition further includes the following components in parts by weight: 0.1-0.5 parts of Artemisia annua tablets.

[0016] Artemisia argyi is a crystalline substance extracted and processed from the fresh leaves of Blumea balsamifera (L.) DC., a plant belonging to the Asteraceae family. Its chemical structure is L-borneol, and it possesses anti-inflammatory, soothing, and absorption-enhancing effects, while also providing a cooling sensation. When combined with other ingredients, it can further promote the absorption of antioxidant and repairing compositions, enhancing their repair effects.

[0017] More preferably, in the above-mentioned antioxidant repair composition, the amount of Artemisia argyi is 0.2 parts by weight.

[0018] Secondly, the present invention provides a method for preparing the above-mentioned antioxidant repair composition, comprising the following steps:

[0019] (1) Mix bamboo fungus with water, heat and soak to obtain a mixture; concentrate the obtained mixture, add ethanol and let stand for more than 8 hours, filter and take the filtrate to obtain bamboo fungus water extract or mix bamboo fungus with ethanol and soak, filter and take the filtrate to obtain bamboo fungus alcohol extract.

[0020] (2) Mix lycopodium with ethanol, reflux to extract the mixture, concentrate the mixture, clarify, filter and take the filtrate to obtain lycopodium extract;

[0021] (3) Mix the sheep ear flower with ethanol, reflux to extract the mixture, concentrate the mixture, clarify, filter and take the filtrate to obtain sheep ear flower extract.

[0022] (4) Weigh out the water-extracted and alcohol-precipitated extract of bamboo fungus or the alcohol-extracted extract of bamboo fungus obtained in step (1), the extract of lycopodium obtained in step (2), the extract of chrysanthemum obtained in step (3) and the artemisia fragrans tablets according to the weight parts, mix them evenly, and the antioxidant repair composition is obtained.

[0023] Preferably, the bamboo fungus extract is extracted using water extraction and alcohol precipitation methods and alcohol extraction methods to remove some high molecular weight polysaccharides and other impurities, ensuring the clarity of the final product.

[0024] The specific steps of the water extraction and alcohol precipitation method are as follows: Clean the bamboo fungus to remove impurities to prevent excessive impurities from adversely affecting the extract. Mix it with water and soak it at a constant temperature of 80-90℃ for at least 2 hours. After extraction, filter the extract cleanly, concentrate it, add 70-95% ethanol, and let it stand to obtain the alcohol precipitate. After filtering the alcohol precipitate of bamboo fungus cleanly, recover the ethanol until there is no alcohol taste to obtain the water-extracted and alcohol-precipitated bamboo fungus extract.

[0025] The specific steps of the alcohol extraction method are as follows: clean and remove impurities from bamboo fungus, mix with 40-70% ethanol, and reflux for extraction; after extraction, recover the ethanol until there is no alcohol odor, then concentrate the extract and clarify; filter and concentrate to obtain bamboo fungus alcohol extract.

[0026] More preferably, the bamboo fungus extract is extracted using a water extraction and alcohol precipitation method.

[0027] Compared with the alcohol extract of bamboo fungus, the water-extraction-alcohol precipitation method yields more active substances in the anti-oxidation and repair composition, thus improving both the antioxidant and repair effects.

[0028] Preferably, the preparation method of the extract of *Hemerocallis fulva* is an alcohol extraction method, which extracts flavonoids from *Hemerocallis fulva*. The specific steps are as follows: the whole plant or roots of *Hemerocallis fulva* are cleaned and impurities are removed, and then mixed with 40-70% ethanol for reflux extraction; after extraction, the ethanol is recovered until there is no alcohol odor, and then the extract is concentrated, clarified, filtered, and concentrated again to obtain the extract of *Hemerocallis fulva*.

[0029] Preferably, the lycopodium extract is obtained by alcohol extraction, which extracts alkaloids and flavonoids from the lycopodium.

[0030] The specific steps are as follows: clean and remove impurities from the lycopodium, mix it with 40-70% ethanol, and reflux for extraction; after extraction, recover the ethanol until there is no alcohol odor, then concentrate the extract, clarify, filter, and concentrate again to obtain the lycopodium extract.

[0031] Thirdly, the present invention provides a cosmetic product comprising the above-described antioxidant repair composition.

[0032] Preferably, the cosmetic product further includes a moisturizer, a preservative, and water; the moisturizer is at least one of glycerin, 1,3-butanediol, and propylene glycol; and the preservative is a compound of 1,2-hexanediol and caprylyl glycol.

[0033] Preferably, the above-mentioned cosmetic comprises the following components in weight percentage: 3%-15% antioxidant repair composition, 5%-50% glycerin, 0%-5% 1,3-butanediol, 0%-45% propylene glycol, 0.525% 1,2-hexanediol, 0.175% caprylyl glycol, and water as the balance.

[0034] Fourthly, the present invention provides a method for preparing the above-mentioned cosmetic, wherein the cosmetic is obtained by uniformly mixing the antioxidant repair composition, moisturizer, preservative and water by weight percentage.

[0035] The advantages of this invention are:

[0036] Current literature primarily focuses on the whitening, anti-aging, and moisturizing effects of bamboo fungus polysaccharides. While bamboo fungus polysaccharides exhibit remarkable performance in these areas, the preparation process requires purification, which not only complicates the extraction process but also wastes other active ingredients. The present invention features a simple preparation process that avoids the use of any toxic or harmful chemicals, ensuring safety, no toxic residues, harmlessness to the human body, and environmental friendliness. The final product is a clear, transparent yellow color, making it easy to apply in cosmetics. Furthermore, the non-single-active-ingredient extraction method gives the bamboo fungus extract of this invention excellent anti-inflammatory and soothing effects.

[0037] However, the repair and antioxidant effects of bamboo fungus extract were not satisfactory. Through screening, bamboo fungus was combined with chrysanthemum and lycopodium, resulting in a synergistic effect with richer total polysaccharides and total flavonoids, and superior antioxidant and repair effects. Attached Figure Description

[0038] Figure 1 This is a standard curve for the determination of total polysaccharide content.

[0039] Figure 2 This is a standard curve for the determination of total triterpenoid content.

[0040] Figure 3 This is a standard curve for the determination of total flavonoid content.

[0041] Figure 4 Flowchart for testing to promote tail fin repair in zebrafish embryos.

[0042] Figure 5 The graph shows the changes before and after testing a zebrafish caudal fin removal model.

[0043] Figure 6 The image shows a comparison of the changes in tail fin length in zebrafish embryos of Example 3 and the blank control group; the top image is the blank control group, and the bottom image is the Example 3 group.

[0044] Figure 7 The image shows a comparison of the changes in tail fin length in zebrafish embryos between Comparative Example 4 and the blank control group; the top image is the blank control group, and the bottom image is the comparative example group. Detailed Implementation

[0045] To better illustrate the purpose, technical solution, and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0046] Appearance and physicochemical properties:

[0047] Appearance and properties: Yellow to brown clear liquid

[0048] Odor: Characteristic odor

[0049] pH value (10% aqueous solution): 3.5–6.0

[0050] Relative density (20℃): 0.9900~1.2520

[0051] Refractive index (20℃): 1.2600~1.5200

[0052] Total triterpenes (%): ≥1.0

[0053] Total polysaccharides (mg / mL): ≥4.0

[0054] Total flavonoids (mg / mL): ≥0.7

[0055] Example 1:

[0056] An embodiment of the antioxidant repair composition of the present invention includes the following components in parts by weight: 5 parts of bamboo fungus extract, 5 parts of lycopodium extract, and 5 parts of chamomile extract.

[0057] The bamboo fungus extract is a water-extracted and alcohol-precipitated extract; the lycopodium extract is an alcohol-extracted extract; and the chamomile extract is an alcohol-extracted extract.

[0058] The preparation method of the above-mentioned antioxidant repair composition includes the following steps:

[0059] (1) Clean the bamboo fungus to remove impurities, mix it with water, and soak it at a constant temperature of 80-90℃ for at least 2 hours. After extraction, filter the extract, concentrate it, add 70-95% ethanol, and let it stand to obtain ethanol precipitate. After filtering the ethanol precipitate of bamboo fungus, recover the ethanol until there is no alcohol taste to obtain the water-extracted ethanol precipitate of bamboo fungus.

[0060] (2) Clean and remove impurities from the lycopodium, mix it with 40-70% ethanol, and reflux for extraction; after extraction, recover the ethanol until there is no alcohol odor, then concentrate the extract, clarify, filter, and concentrate again to obtain the lycopodium extract;

[0061] (3) Clean the whole plant or roots of the sheep ear flower to remove impurities, mix with 40-70% ethanol, and reflux for extraction; after extraction, recover the ethanol until there is no alcohol taste, then concentrate the extract, clarify, filter, and concentrate again to obtain the sheep ear flower extract.

[0062] (4) Weigh out the bamboo fungus water extract obtained in step (1), the lycopodium extract obtained in step (2), and the chrysanthemum extract obtained in step (3) according to the weight parts, mix them evenly, and the antioxidant repair composition is obtained.

[0063] A cosmetic product comprising the following components in weight percentage: 15% antioxidant repair composition, 50% glycerin, 0.525% 1,2-hexanediol, 0.175% caprylyl glycol, and water as the balance.

[0064] The cosmetic product can be obtained by mixing the above components evenly according to the weight percentage.

[0065] Example 2:

[0066] An embodiment of the antioxidant repair composition of the present invention includes the following components in parts by weight: 5 parts of bamboo fungus extract, 5 parts of lycopodium extract, and 5 parts of chamomile extract.

[0067] The bamboo fungus extract is an alcohol extract; the lycopodium extract is an alcohol extract; and the chamomile extract is an alcohol extract.

[0068] The preparation method of the above-mentioned antioxidant repair composition includes the following steps:

[0069] (1) Clean and remove impurities from bamboo fungus, mix with 40-70% ethanol, and reflux for extraction; after extraction, recover the ethanol until there is no alcohol taste, then concentrate the extract and clarify; filter and concentrate to obtain bamboo fungus alcohol extract;

[0070] (2) Clean and remove impurities from the lycopodium, mix it with 40-70% ethanol, and reflux for extraction; after extraction, recover the ethanol until there is no alcohol odor, then concentrate the extract, clarify, filter, and concentrate again to obtain the lycopodium extract;

[0071] (3) Clean the whole plant or roots of the sheep ear flower to remove impurities, mix with 40-70% ethanol, and reflux for extraction; after extraction, recover the ethanol until there is no alcohol taste, then concentrate the extract, clarify, filter, and concentrate again to obtain the sheep ear flower extract.

[0072] (4) Weigh out the bamboo fungus alcohol extract obtained in step (1), the lycopodium extract obtained in step (2), and the chamomile extract obtained in step (3) according to the weight parts, mix them evenly, and the antioxidant repair composition is obtained.

[0073] A cosmetic product comprising the following components in weight percentage: 15% antioxidant repair composition, 50% glycerin, 0.525% 1,2-hexanediol, 0.175% caprylyl glycol, and water as the balance.

[0074] The cosmetic product can be obtained by mixing the above components evenly according to the weight percentage.

[0075] Example 3:

[0076] An embodiment of the antioxidant repair composition of the present invention includes the following components in parts by weight: 1 part of bamboo fungus extract, 1 part of lycopodium extract, and 1 part of chamaedorea extract.

[0077] The bamboo fungus extract is a water-extracted and alcohol-precipitated extract; the lycopodium extract is an alcohol-extracted extract; and the chamomile extract is an alcohol-extracted extract.

[0078] The preparation method of the above-mentioned antioxidant repair composition is the same as that in Example 1.

[0079] A cosmetic product comprising the following components in weight percentage: 3% antioxidant repair composition, 50% glycerin, 0.525% 1,2-hexanediol, 0.175% caprylyl glycol, and water as the balance.

[0080] The cosmetic product can be obtained by mixing the above components evenly according to the weight percentage.

[0081] Example 4:

[0082] An embodiment of the antioxidant repair composition of the present invention comprises the following components in parts by weight: 5 parts of bamboo fungus extract, 5 parts of lycopodium extract, 5 parts of chamomile extract, and 0.2 parts of artemisia.

[0083] The bamboo fungus extract is an alcohol extract; the lycopodium extract is an alcohol extract; and the chamomile extract is an alcohol extract.

[0084] The preparation method of the above-mentioned antioxidant repair composition includes the following steps:

[0085] (1) Clean and remove impurities from bamboo fungus; mix with 40-70% ethanol and reflux for extraction; after extraction, recover the ethanol until there is no alcohol taste, then concentrate the extract and clarify; filter and concentrate to obtain bamboo fungus alcohol extract;

[0086] (2) Clean and remove impurities from the lycopodium, mix it with 40-70% ethanol, and reflux for extraction; after extraction, recover the ethanol until there is no alcohol odor, then concentrate the extract, clarify, filter, and concentrate again to obtain the lycopodium extract;

[0087] (3) Clean the whole plant or roots of the sheep ear flower to remove impurities, mix with 40-70% ethanol, and reflux for extraction; after extraction, recover the ethanol until there is no alcohol taste, then concentrate the extract, clarify, filter, and concentrate again to obtain the sheep ear flower extract.

[0088] (4) Weigh out the bamboo fungus alcohol extract obtained in step (1), the lycopodium extract obtained in step (2), the chamomile extract obtained in step (3), and the artemisia fragrans tablets according to the weight parts, mix them evenly, and the antioxidant repair composition is obtained.

[0089] A cosmetic product comprising the following components by weight percentage: 15.2% antioxidant repair composition, 30% glycerin, 0.525% 1,2-hexanediol, 0.175% caprylyl glycol, 45% propylene glycol, and water as the balance.

[0090] The cosmetic product can be obtained by mixing the above components evenly according to the weight percentage.

[0091] Example 5:

[0092] An embodiment of the antioxidant repair composition of the present invention comprises the following components in parts by weight: 1 part bamboo fungus extract, 1 part lycopodium extract, 1 part chamaejasme extract, and 0.2 parts artemisia argyi.

[0093] The bamboo fungus extract is a water-extracted and alcohol-precipitated extract; the lycopodium extract is an alcohol-extracted extract; and the chamomile extract is an alcohol-extracted extract.

[0094] The preparation method of the above-mentioned antioxidant repair composition includes the following steps:

[0095] (1) Clean the bamboo fungus to remove impurities, mix it with water, and soak it at a constant temperature of 80-90℃ for at least 2 hours. After extraction, filter the extract, concentrate it, add 70-95% ethanol, and let it stand to obtain ethanol precipitate. After filtering the ethanol precipitate of bamboo fungus, recover the ethanol until there is no alcohol taste to obtain the water-extracted ethanol precipitate of bamboo fungus.

[0096] (2) Clean and remove impurities from the lycopodium, mix it with 40-70% ethanol, and reflux for extraction; after extraction, recover the ethanol until there is no alcohol odor, then concentrate the extract, clarify, filter, and concentrate again to obtain the lycopodium extract;

[0097] (3) Clean the whole plant or roots of the sheep ear flower to remove impurities, mix with 40-70% ethanol, and reflux for extraction; after extraction, recover the ethanol until there is no alcohol taste, then concentrate the extract, clarify, filter, and concentrate again to obtain the sheep ear flower extract.

[0098] (4) Weigh out the bamboo fungus water extract obtained in step (1), the lycopodium extract obtained in step (2), the chamomile extract obtained in step (3), and the artemisia fragrans tablets according to the weight parts, mix them evenly, and the antioxidant repair composition is obtained.

[0099] A cosmetic product comprising the following components by weight percentage: 3.2% antioxidant repair composition, 45% glycerin, 0.525% 1,2-hexanediol, 0.175% caprylyl glycol, 5% butylene glycol, and water as the balance.

[0100] The cosmetic product can be obtained by mixing the above components evenly according to the weight percentage.

[0101] Example 6:

[0102] An embodiment of the antioxidant repair composition of the present invention includes the following components in parts by weight: 1 part of bamboo fungus extract, 1.1 parts of lycopodium extract, and 1.1 parts of chamaedorea extract.

[0103] The bamboo fungus extract is a water-extracted and alcohol-precipitated extract; the lycopodium extract is an alcohol-extracted extract; and the chamomile extract is an alcohol-extracted extract.

[0104] The preparation method of the above-mentioned antioxidant repair composition is the same as that in Example 1.

[0105] A cosmetic product comprising the following components in weight percentage: 3% antioxidant repair composition, 50% glycerin, 0.525% 1,2-hexanediol, 0.175% caprylyl glycol, and water as the balance.

[0106] The cosmetic product can be obtained by mixing the above components evenly according to the weight percentage.

[0107] Example 7:

[0108] An embodiment of the antioxidant repair composition of the present invention comprises the following components in parts by weight: 1 part of bamboo fungus extract, 1 part of lycopodium extract, 1 part of chamaejasminoides extract, and 0.1 part of artemisia argyi.

[0109] The bamboo fungus extract is a water-extracted and alcohol-precipitated extract; the lycopodium extract is an alcohol-extracted extract; and the chamomile extract is an alcohol-extracted extract.

[0110] The preparation method of the above-mentioned antioxidant repair composition is the same as that in Example 5.

[0111] A cosmetic product comprising the following components by weight percentage: 3.1% antioxidant repair composition, 45% glycerin, 0.525% 1,2-hexanediol, 0.175% caprylyl glycol, 5% butylene glycol, and water as the balance.

[0112] The cosmetic product can be obtained by mixing the above components evenly according to the weight percentage.

[0113] Example 8:

[0114] An embodiment of the antioxidant repair composition of the present invention comprises the following components in parts by weight: 1 part bamboo fungus extract, 1 part lycopodium extract, 1 part chamaejasme extract, and 0.5 parts artemisia argyi.

[0115] The bamboo fungus extract is a water-extracted and alcohol-precipitated extract; the lycopodium extract is an alcohol-extracted extract; and the chamomile extract is an alcohol-extracted extract.

[0116] The preparation method of the above-mentioned antioxidant repair composition is the same as that in Example 5.

[0117] A cosmetic product comprising the following components by weight percentage: 3.5% antioxidant repair composition, 45% glycerin, 0.525% 1,2-hexanediol, 0.175% caprylyl glycol, 5% butylene glycol, and water as the balance.

[0118] The cosmetic product can be obtained by mixing the above components evenly according to the weight percentage.

[0119] Comparative Example 1:

[0120] A comparative example of the antioxidant repair composition of the present invention comprises the following components in parts by weight: 1 part of lycopodium extract and 1 part of chamaedorea extract.

[0121] The lycopodium extract is an alcohol extract; the chrysanthemum extract is an alcohol extract.

[0122] The difference between the preparation method of the above-mentioned antioxidant repair composition and Example 3 is that bamboo fungus extract is missing.

[0123] A cosmetic product comprising the following components in weight percentage: 2% antioxidant repair composition, 50% glycerin, 0.525% 1,2-hexanediol, 0.175% caprylyl glycol, and water as the balance.

[0124] The cosmetic product can be obtained by mixing the above components evenly according to the weight percentage.

[0125] Comparative Example 2:

[0126] A comparative example of the antioxidant repair composition of the present invention comprises the following components in parts by weight: 1 part of bamboo fungus extract and 1 part of chamomile extract.

[0127] The bamboo fungus extract is a water-extracted and alcohol-precipitated extract; the sheep ear fungus extract is an alcohol-extracted extract.

[0128] The difference between the preparation method of the above-mentioned antioxidant repair composition and that of Example 3 is that the lycopodium extract is missing.

[0129] A cosmetic product comprising the following components in weight percentage: 2% antioxidant repair composition, 50% glycerin, 0.525% 1,2-hexanediol, 0.175% caprylyl glycol, and water as the balance.

[0130] The cosmetic product can be obtained by mixing the above components evenly according to the weight percentage.

[0131] Comparative Example 3:

[0132] A comparative example of the antioxidant repair composition of the present invention comprises the following components in parts by weight: 1 part of bamboo fungus extract and 1 part of lycopodium extract.

[0133] The bamboo fungus extract is a water-extracted and alcohol-precipitated extract; the lycopodium extract is an alcohol-extracted extract.

[0134] The difference between the preparation method of the above antioxidant repair composition and Example 3 is that the okra extract is missing.

[0135] A cosmetic product comprising the following components in weight percentage: 2% antioxidant repair composition, 50% glycerin, 0.525% 1,2-hexanediol, 0.175% caprylyl glycol, and water as the balance.

[0136] The cosmetic product can be obtained by mixing the above components evenly according to the weight percentage.

[0137] Comparative Example 4:

[0138] A comparative example of the antioxidant repair composition of the present invention includes the following components in parts by weight: 1 part of bamboo fungus extract.

[0139] The bamboo fungus extract is a water-extracted and alcohol-precipitated extract.

[0140] The difference between the preparation method of the above antioxidant repair composition and Example 3 is that it lacks lycopodium extract and chamomile extract.

[0141] A cosmetic product comprising the following components by weight percentage: 1% antioxidant repair composition, 50% glycerin, 0.525% 1,2-hexanediol, 0.175% caprylyl glycol, and water as the balance.

[0142] The cosmetic product can be obtained by mixing the above components evenly according to the weight percentage.

[0143] Comparative Example 5:

[0144] A comparative example of the antioxidant repair composition of the present invention comprises the following components in parts by weight: 0.1 parts of bamboo fungus extract, 0.1 parts of lycopodium extract, and 0.1 parts of chamomile extract.

[0145] The bamboo fungus extract is a water-extracted and alcohol-precipitated extract; the lycopodium extract is an alcohol-extracted extract; and the chamomile extract is an alcohol-extracted extract.

[0146] The preparation method of the above-mentioned antioxidant repair composition is the same as that in Example 1.

[0147] A cosmetic product comprising the following components by weight percentage: 0.3% antioxidant repair composition, 50% glycerin, 0.525% 1,2-hexanediol, 0.175% caprylyl glycol, and water as the balance.

[0148] The cosmetic product can be obtained by mixing the above components evenly according to the weight percentage.

[0149] Comparative Example 6

[0150] A comparative example of the antioxidant repair composition of the present invention comprises the following components in parts by weight: 0.1 parts of bamboo fungus extract, 0.1 parts of lycopodium extract, and 0.1 parts of chamomile extract.

[0151] The bamboo fungus extract is an alcohol extract; the lycopodium extract is an alcohol extract; and the chamomile extract is an alcohol extract.

[0152] The preparation method of the above-mentioned antioxidant repair composition is the same as that in Example 2.

[0153] A cosmetic product comprising the following components by weight percentage: 0.3% antioxidant repair composition, 50% glycerin, 0.525% 1,2-hexanediol, 0.175% caprylyl glycol, and water as the balance.

[0154] The cosmetic product can be obtained by mixing the above components evenly according to the weight percentage.

[0155] Comparative Example 7:

[0156] A comparative example of the antioxidant repair composition of the present invention comprises the following components in parts by weight: 0.1 parts of bamboo fungus extract, 0.1 parts of lycopodium extract, 0.1 parts of chamaejasminoides extract, and 0.2 parts of artemisia argyi.

[0157] The bamboo fungus extract is a water-extracted and alcohol-precipitated extract; the lycopodium extract is an alcohol-extracted extract; and the chamomile extract is an alcohol-extracted extract.

[0158] The preparation method of the above-mentioned antioxidant repair composition is the same as that in Example 5.

[0159] A cosmetic product comprising the following components by weight percentage: 0.5% antioxidant repair composition, 30% glycerin, 0.525% 1,2-hexanediol, 0.175% caprylyl glycol, 20% propylene glycol, and water as the balance.

[0160] The cosmetic product can be obtained by mixing the above components evenly according to the weight percentage.

[0161] Comparative Example 8:

[0162] A comparative example of the antioxidant repair composition of the present invention comprises the following components in parts by weight: 0.2 parts of bamboo fungus extract, 0.3 parts of lycopodium extract, and 0.3 parts of chamomile extract.

[0163] The bamboo fungus extract is a water-extracted and alcohol-precipitated extract; the lycopodium extract is an alcohol-extracted extract; and the chamomile extract is an alcohol-extracted extract.

[0164] The preparation method of the above-mentioned antioxidant repair composition is the same as that in Example 1.

[0165] A cosmetic product comprising the following components in weight percentages: 0.% antioxidant repair composition, 50% glycerin, 0.525% 1,2-hexanediol, 0.175% caprylyl glycol, and water as the balance.

[0166] The cosmetic product can be obtained by mixing the above components evenly according to the weight percentage.

[0167] Test Example 1 – Determination of Total Polysaccharide Content (Phenol-Sulfuric Acid Method)

[0168] Experimental methods:

[0169] 1. Preparation of reagents

[0170] (1) 5% phenol solution: Weigh 2.5g of phenol, add water to make up to 50mL, and mix well.

[0171] (2) Glucose standard stock solution: Weigh 4.5 mg of glucose and dilute to 50 mL in a volumetric flask (accurate dilution, store at 4℃).

[0172] (3) Sample stock solution: (sample diluted 1000 times: 10uL sample + 9mL water + 990uL water) Accurately measure 1mL of the diluted sample and place it in a dry 10mL stoppered test tube.

[0173] 2. Preparation of standard curve

[0174] Accurately measure 0.2 mL, 0.4 mL, 0.6 mL, 0.8 mL, and 1.0 mL of the reference solution into 10 mL stoppered test tubes, and add double-distilled water to each tube to a final volume of 1.0 mL (add water first, then the stock solution). Prepare solutions with concentrations of 0, 18, 36, 54, 72, and 90 μg / mL, respectively. Perform a phenol-sulfuric acid colorimetric reaction. Plot a standard curve with absorbance on the ordinate and concentration (actual weighing and dilution concentration) on the abscissa. The standard curve is shown below. Figure 1 As shown.

[0175] 3. Sample determination

[0176] Accurately measure 1.0 mL each of a series of glucose standard solutions, sample solutions, and double-distilled water, and place them in 10 mL stoppered test tubes (ensure the stoppered test tubes are dry and free of water, otherwise the concentration will change). Accurately add 1 mL of 5% phenol solution (prepare immediately before use) and vortex to mix. Then accurately add 5 mL of sulfuric acid, seal with sealing film, vortex to mix, and heat in a boiling water bath at 100°C for 20 minutes (time is accurate; use a timer for simultaneous timing; ensure the water does not cover the reaction solution and check for leaks at the bottom of the stoppered test tube). Remove the tube, cool it in an ice bath for 5 minutes, vortex to mix, and allow it to stand until no bubbles remain. Measure the absorbance at a wavelength of 488 nm using a UV spectrophotometer. Read the amount of anhydrous glucose in the test solution from the standard curve and calculate using formula (1). The results are shown in Table 1.

[0177] Concentration (mg / mL) = C sample * dilution factor * 10 -3 Equation (1)

[0178] Table 1

[0179]

[0180]

[0181] As shown in the table above, the total polysaccharide content of Examples 1-8 is higher than that of Comparative Examples 1-4, indicating that the absence of any one of the bamboo fungus extract, lycopodium extract, and chamomile extract will lead to a decrease in the total polysaccharide content. The total polysaccharide content of Examples 1-8 is higher than that of Comparative Examples 5-8, indicating that the weight proportions of bamboo fungus extract, lycopodium extract, and chamomile extract exceed the scope of this invention, resulting in a decrease in the total polysaccharide content.

[0182] Example 1 had the highest total polysaccharide content. Compared to Example 1, Example 3 had a similar enrichment of active ingredients, but used less raw materials, making it the optimal example. Examples 4 and 5 added Artemisia argyi as a component, but had little effect on the total polysaccharide content.

[0183] Compared to Example 2, under the same weight, the bamboo fungus extract and lycopodium extract obtained by water extraction and alcohol precipitation, when combined with the chamaedorea extract, had a higher total polysaccharide content than the alcohol-extracted bamboo fungus extract. This high total polysaccharide content provides a material basis for the antioxidant repair composition to achieve high anti-inflammatory and antioxidant effects.

[0184] Test Example 2 – Determination of Total Triterpenoid Content (Vanillin-Concentrated Sulfuric Acid Method)

[0185] Experimental methods:

[0186] 1. Preparation of reagents

[0187] Reagents and reagents: Prepare analytical grade methanol, chloroform, concentrated sulfuric acid, vanillin, and ursolic acid standards.

[0188] 2. Solution preparation:

[0189] (1) Vanillin solution: Weigh 4.0g vanillin, dissolve it in methanol, dilute to 50mL in a volumetric flask, and shake well.

[0190] (2) 72% sulfuric acid solution: Take 122 mL of water, slowly add 288 mL of concentrated sulfuric acid, and mix well.

[0191] (3) Ursolic acid standard stock solution: Accurately weigh 2 mg of ursolic acid standard, add 10 mL of chloroform to prepare a solution containing 0.2 mg of ursolic acid per 1 mL.

[0192] 3. Testing Methods

[0193] (1) Preparation of the standard curve: Accurately measure 0, 0.07 mL, 0.14 mL, 0.21 mL, 0.28 mL, 0.35 mL, 0.42 mL, and 0.49 mL of the standard solution into 10 mL stoppered test tubes. Evaporate the solvent in a water bath at 80 °C, cool, and accurately add 0.5 mL of freshly prepared vanillin solution and 5 mL of 72% sulfuric acid. Vortex to mix, heat in a 60 °C water bath for 30 minutes, immediately cool in an ice bath for 15 minutes, remove, vortex to mix, and measure the absorbance at a wavelength of 548 nm. Plot the standard curve with absorbance as the ordinate and concentration as the abscissa. The standard curve is shown below. Figure 2 As shown.

[0194] (2) Determination of extract content: Following the method under the preparation of the standard curve, take 0.1 mL of sample solution (the dilution factor was determined in the preliminary experiment) and place it in a 10 mL stoppered test tube. Following the method under the preparation of the standard curve, starting from "evaporating the solvent in a water bath", operate in the same way and measure the absorbance. Read the content of ursolic acid in the sample solution from the standard curve and calculate it according to formula (2). The results are shown in Table (2).

[0195] Triterpenoid content = Csample * dilution factor * 0.5 / Vsample (Formula 2)

[0196] Table 2

[0197]

[0198]

[0199] As shown in the table above, the total triterpenoid content of Examples 1-8 is higher than that of Comparative Example 1, indicating that the lack of bamboo fungus extract leads to a decrease in the total triterpenoid content; the total triterpenoid content is related to the content of bamboo fungus extract. The total triterpenoid content of Examples 1-8 is higher than that of Comparative Examples 5-8, indicating that the weight proportions of bamboo fungus extract, lycopodium extract, and chamomile extract exceed the scope of this invention, resulting in a decrease in the total triterpenoid content.

[0200] Example 1 has the highest total triterpenoid content. Compared with Example 1, Example 3 has a similar enrichment of active ingredients, but uses less raw materials. In Example 4, the weight proportions of bamboo fungus extract, lycopodium extract, and chamomile extract are relatively large, making them prone to precipitation and resulting in an unstable system.

[0201] Compared with Example 2, the bamboo fungus extract and lycopodium extract obtained by water extraction and alcohol precipitation, when combined with the chamaedorea extract, had a higher total triterpenoid content than the alcohol-extracted bamboo fungus extract, under the same weight parts.

[0202] Test Example 3 – Determination of Total Flavonoid Content

[0203] Experimental methods:

[0204] 1. Preparation of reagents

[0205] (1) 5% sodium nitrite solution: Weigh 5.0g of sodium nitrite, dissolve it in purified water, make up to 100mL in a volumetric flask, shake well, and store in the dark.

[0206] (2) 10% aluminum nitrate solution: Weigh 10.0g of aluminum nitrate, dissolve it in purified water, make up to 100mL in a volumetric flask, shake well, and store in the dark.

[0207] (3) 4% sodium hydroxide solution: Weigh 4.0g of sodium hydroxide, dissolve it in purified water, make up to 100mL in a volumetric flask, shake well, and store.

[0208] (4) 70% ethanol: Measure 70 mL of anhydrous ethanol, add water to make up to 100 mL, and mix well.

[0209] 2. Preparation of reference solution

[0210] Accurately weigh 5 mg of rutin reference standard dried to constant weight at 120℃, place it in a 50 mL brown volumetric flask, add an appropriate amount of 70% ethanol (30 mL), heat gently in a water bath or sonicate to dissolve the powder, cool, then add 70% ethanol to the mark, shake well, and you will get the solution (each 1 mL of solution contains 0.1 mg of anhydrous rutin).

[0211] 3. Preparation of standard lines

[0212] Accurately pipette 0 mL, 1 mL, 2 mL, 3 mL, 4 mL, and 5 mL of rutin standard solution into separate 10 mL volumetric flasks. Add 70% ethanol to each flask to a final volume of 5 mL and mix well. Add 0.3 mL of 5% sodium nitrite solution, mix well, and let stand for 6 min. Add 0.3 mL of 10% aluminum nitrate solution, mix well, and let stand for 6 min. Add 4 mL of 4% sodium hydroxide solution, and dilute to the mark with 70% ethanol. Let stand for 15 min. Using a reagent blank as a reference, immediately measure the absorbance at 510 nm using UV-Vis spectrophotometry. Plot a standard curve with absorbance on the ordinate and concentration on the abscissa. The standard curve is shown below. Figure 3 As shown.

[0213] 4. Sample determination

[0214] Take 1 mL of sample solution and place it in a 10 mL volumetric flask. Add 0.3 mL of 5% sodium nitrite solution, mix well, and let stand for 6 min. Add 0.3 mL of 10% aluminum nitrate solution, mix well, and let stand for 6 min. Then add 4.0 mL of 4% sodium hydroxide solution, add 70% ethanol to the mark, and let stand for 15 min. Using the reagent blank as a reference, measure the absorbance at a wavelength of 510 nm using ultraviolet-visible spectrophotometry. Read the rutin content in the test solution from the standard curve, and calculate the total flavonoid content in the sample according to formula (3). The results are shown in Table 3.

[0215] Concentration (mg / mL) = C sample * dilution factor * 10 -3 / Sample addition volume formula (3)

[0216] Table 3

[0217]

[0218]

[0219] As shown in the table above, the total flavonoid content of Examples 1-8 is higher than that of Comparative Examples 1-4, indicating that the absence of any one of the bamboo fungus extract, lycopodium extract, and chamomile extract will lead to a decrease in the total flavonoid content. The total flavonoid content of Examples 1-8 is higher than that of Comparative Examples 5-8, indicating that the weight proportions of bamboo fungus extract, lycopodium extract, and chamomile extract exceed the scope of this invention, resulting in a decrease in the total flavonoid content.

[0220] Although Example 1 had the highest total flavonoid content, it was prone to precipitation. Compared with Example 1, Example 3 had a similar enrichment of active ingredients, used less raw materials, was more cost-effective, and had a more stable system, thus it was the best example.

[0221] Compared to Example 2, under the same weight parts, the bamboo fungus extract and lycopodium extract obtained by water extraction and alcohol precipitation, when combined with the extract of *Heliotropium indicum*, had a higher total flavonoid content than the alcohol-extracted bamboo fungus extract. The bamboo fungus extract in Comparative Example 4, without any other components, had the lowest total flavonoid content.

[0222] Test Example 4 – Antioxidant Capacity Test (Scavenging DPPH Free Radicals)

[0223] Antioxidant activity can be characterized by evaluating the inhibition rate of DPPH free radicals in test samples. DPPH analysis is a simple method for screening free radical scavengers and evaluating antioxidant activity. DPPH in ethanol is purple, with a maximum absorption wavelength of 517 nm. When a free radical scavenger is added to the DPPH solution, its lone pairs of electrons are paired, causing the absorption to disappear or weaken, resulting in a lighter solution color, appearing yellow or pale yellow, and a decrease in absorbance at 517 nm. The degree of change is linearly related to the free radical scavenging intensity, thus allowing for quantitative analysis using spectrophotometry. The lower the sample concentration achieving a 50% DPPH free radical scavenging rate, i.e., the lower the half-maximal scavenging rate (EC50), the stronger the free radical scavenging ability of the substance.

[0224] Experimental methods:

[0225] 1. Preparation of reagents

[0226] Solution preparation:

[0227] 0.05 mmol / L DPPH solution: Weigh 0.0020 g DPPH, dissolve it thoroughly in anhydrous ethanol, and bring the volume to 100 mL. Store protected from light and use within 3.5 hours.

[0228] Sample solution: Dissolve the sample in a suitable solvent (choose according to polarity, anhydrous ethanol is preferred), and prepare it to a concentration of 1 mg / mL for easy calculation.

[0229] 2. Preliminary Experiment

[0230] Take 3.5 mL of DPPH solution in a test tube, add a small amount of sample solution gradually, starting with a small amount and gradually increasing, mixing as you add. Observe the fading of the solution color. When the solution color has basically faded, record the amount of sample added. This amount is the maximum sample volume. Based on this maximum volume, set 5 volumes forward to form an arithmetic progression. (For example, X: 200, 160, 120, 80, 40 μL, sample solvent: (1000 μL - x). If the total volume is 9 mL, then 7 mL of DPPH free radical working solution and 2 mL of sample solution are used, and so on.)

[0231] 3. Measurement Method

[0232] 1) Absorbance A: Take 3.5 mL of DPPH solution, add sample solutions of different concentrations, mix immediately, let stand in the dark at room temperature for 30 min, and measure the absorbance A at a wavelength of 517 nm.

[0233] 2) Absorbance A1: Measure the absorbance of 1 mL of sample solution of different concentrations and 3.5 mL of anhydrous alcohol solution at a wavelength of 517 nm (this step can be omitted if the sample does not absorb at 517 nm).

[0234] 3) Absorbance A0: Measure the absorbance of 3.5 mL of DPPH solution and 1 mL of 70% ethanol at a wavelength of 517 nm. Perform the operation 2-3 times in parallel for each concentration and take the average value.

[0235] 4. Clearance rate calculation

[0236] The DPPH radical scavenging rate is calculated according to formula (4) or formula (5):

[0237] Clearance rate ROS (%) = [1 - (A - A1 / A0)] × 100% Equation (4)

[0238] Removal rate ROS (%) = (AO - A / A0) × 100% Equation (5)

[0239] In formula (4): A is the absorbance of the sample reacting with DPPH, A1 is the control absorbance of anhydrous ethanol instead of DPPH, and AO is the blank absorbance of 70% ethanol instead of the sample.

[0240] In formula (5): A0 is the absorbance of distilled water as a blank, and A is the absorbance after adding the sample.

[0241] The results are shown in Table 4.

[0242] Table 4

[0243]

[0244]

[0245] As shown in the table above, the half-maximal clearance rates of Examples 1-8 are all lower than those of Comparative Examples 1-4, indicating that the absence of any one of the bamboo fungus extract, lycopodium extract, and chamomile extract will lead to an increase in the half-maximal clearance rate and a decrease in antioxidant capacity. The half-maximal clearance rates of Examples 1-8 are all lower than those of Comparative Examples 5-8, indicating that the weight proportions of bamboo fungus extract, lycopodium extract, and chamomile extract exceed the scope of this invention, resulting in an increase in the half-maximal clearance rate and a decrease in antioxidant capacity.

[0246] Compared to Example 2, under the same weight parts, the bamboo fungus extract and lycopodium extract obtained by water extraction and alcohol precipitation, when combined with the chamaedorea extract, showed a lower half-maximal scavenging rate and stronger antioxidant capacity compared to the alcohol-extracted bamboo fungus extract. The combination of water-extracted bamboo fungus extract, lycopodium extract, and chamaedorea extract can increase the antioxidant capacity of the DPPH free radical model of the antioxidant repair composition.

[0247] Test Example 5 – Zebrafish Tail Fin Repair Effect

[0248] When injured, the skin must regenerate rapidly to repair the skin barrier. In the embryonic stage, wound healing is very rapid and leaves no scar; however, after the embryonic stage, wound healing involves steps such as coagulation, inflammation, skin regeneration, blood vessel regeneration, and the formation of granulation tissue, ultimately resulting in a scar. In zebrafish, the wound repair process is identical to that in humans, except for the absence of coagulation. Zebrafish wounds heal very quickly, followed by the migration of inflammatory cells to the wound to form granulation tissue composed of macrophages, fibroblasts, blood vessels, and collagen. Therefore, the main steps and principles of wound healing in zebrafish and humans are very similar, making zebrafish a suitable model for screening and evaluating the effectiveness of human skin repair.

[0249] Repair effect: Tested using a zebrafish embryo caudal fin excision model.

[0250] Experimental methods:

[0251] A zebrafish embryonic caudal fin removal model was used for testing. Twenty-four caudal fin-removed fish embryos were exposed to a sample solution with a 5% concentration of the formulated additive. A control group was also included. After 48 hours of exposure, the fish embryos were photographed under a microscope, and the caudal fin regeneration length was measured and statistically analyzed. The caudal fin repair promotion rate of each group of samples at a 5% formulation concentration was compared.

[0252] The flowchart for testing the zebrafish embryo caudal fin removal model is as follows: Figure 4 As shown.

[0253] Testing steps: Select healthy zebrafish embryos that are 3 days old after fertilization.

[0254] 1. Establish a damage model

[0255] Zebrafish embryos were anesthetized with tricaine solution, and their tail fins were removed under a microscope using a scalpel. Figure 4 ).

[0256] 2. Test Grouping

[0257] The test requires setting up a model control group (fish embryo culture medium / solvent solution), a positive control group (Rehmannia glutinosa extract), and a test substance group (test substance).

[0258] 2.1 Model control group setup

[0259] Twenty-four fish embryos were randomly selected and placed into a 96-well plate, with each well containing one fish embryo and 0.2 mL of fish embryo culture medium.

[0260] 2.2 Setting up a positive control group

[0261] Twenty-four fish embryos were randomly selected and placed into a 96-well plate, with each well containing one fish embryo and 0.2 mL of Rehmannia glutinosa extract solution.

[0262] 2.3 Test substance treatment

[0263] Twenty-four fish embryos were randomly selected and placed into a 96-well plate, with each well containing one fish embryo and 0.2 mL of the test solution.

[0264] Incubate in a constant temperature incubator at 28℃±1℃ for 48h±1h.

[0265] 3. Microscopic analysis of the samples

[0266] The zebrafish were anesthetized with tricaine, and then the tail of the embryo was photographed from the side under a stereomicroscope.

[0267] 4. Data and Result Calculation

[0268] (1) Calculate the tail fin length of each fish embryo, see Figure 3 .

[0269] (2) The calculation of the tail fin repair promotion rate is shown in equation (6):

[0270] Promotion rate = (SC) / C × 100% Equation (6)

[0271] In equation (6), S is the average length of the caudal fin of the fish embryos in the test group; C is the average length of the caudal fin of the fish embryos in the model control group.

[0272] The mean and standard error of each experimental group were calculated. The statistical results were expressed as mean ± standard error. Statistical software was used to perform analysis of variance on the data. A two-tailed t-test was performed on the caudal fin length between the test group and the model control group to obtain the p-value. p < 0.05 indicated a significant difference.

[0273] 5. Test Validation

[0274] (1) Test judgment: At least 90% of the fish embryos in each test group survived after exposure; otherwise, the results of the corresponding test group are invalid.

[0275] (2) Testing requirements: A positive control group must be set up for each batch of tests. The positive control group fish embryo tail fin repair promotion rate must be positive and p<0.05.

[0276] The results are shown in Table 5.

[0277] Table 5

[0278] Group Zebrafish embryo tail fin repair promotion rate Example 1 24％ Example 2 23％ Example 3 23％ Example 4 28％ Example 5 28％ Example 6 25％ Example 7 25％ Example 8 26％ Comparative Example 1 15％ Comparative Example 2 11％ Comparative Example 3 12％ Comparative Example 4 12％ Comparative Example 5 5％ Comparative Example 6 5％ Comparative Example 7 5％ Comparative Example 8 8％

[0279] As shown in the table above, the repair promotion rate of zebrafish embryo tail fins in Examples 1-8 was higher than that in Comparative Examples 1-4, indicating that the lack of any one of bamboo fungus extract, lycopodium extract and chamomile extract would lead to a decrease in the repair promotion rate of zebrafish embryo tail fins and a decrease in the repair effect. Figure 6 This is a comparison diagram of the changes in caudal fin length in zebrafish embryos of Example 3 and the blank control group. Figure 7 The image shows a comparison of the changes in tail fin length in zebrafish embryos between Comparative Example 4 and the blank control group. It can be seen that both Example 3 and Comparative Example 4 contain bamboo fungus extract, which has a repairing effect. However, the bamboo fungus extract, lycopodium extract, and chamomile extract in Example 3 have a synergistic effect, which significantly promotes the repair effect.

[0280] The repair promotion rate of zebrafish embryo tail fins in Examples 1-8 was lower than that in Comparative Examples 5-8, indicating that the weight proportions of bamboo fungus extract, lycopodium extract and chamomile extract exceeded the scope of this invention, resulting in a decrease in the repair promotion rate of zebrafish embryo tail fins and a decrease in the repair effect.

[0281] When the remaining components are in the same weight proportions, compared with Examples 1 and 2, and with Examples 3 and 5, the addition of Artemisia argyi increased the zebrafish embryo tail fin repair promotion rate by 5%, enhancing the repair effect. Examples 4 and 5, with the addition of Artemisia argyi as a component, showed even better zebrafish embryo tail fin repair promotion rates. Artemisia argyi can enhance penetration and absorption, promoting the repair effect. Based on product stability and cost-effectiveness in R&D, Examples 3 and 5 were selected as the preferred options.

[0282] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. An antioxidant repair composition, characterized in that, The product contains the following components in parts by weight: 1-5 parts bamboo fungus extract, 1-5 parts lycopodium extract, and 1-5 parts chamomile extract. The preparation method of the bamboo fungus extract includes the following steps: Bamboo fungus is cleaned and impurities removed, mixed with water, and soaked at a constant temperature of 80-90℃ for at least 2 hours; after extraction, the extract is filtered cleanly, concentrated, and 70-95% ethanol is added, followed by ethanol precipitation and standing to obtain an ethanol precipitate; the ethanol precipitate of bamboo fungus is filtered cleanly, and the ethanol is recovered until no alcohol odor is detected to obtain the bamboo fungus water-extracted ethanol-precipitated extract; or, bamboo fungus is cleaned and impurities removed, mixed with 40-70% ethanol, and refluxed for extraction; after extraction, the ethanol is recovered until no alcohol odor is detected, and then the extract is concentrated and clarified; filtered and concentrated, the bamboo fungus ethanol extract is obtained. The preparation method of the lycopodium extract includes the following steps: cleaning and removing impurities from the lycopodium, mixing it with 40-70% ethanol, and reflux extraction; after extraction, recovering the ethanol until there is no alcohol odor, then concentrating the extract, clarifying, filtering, and concentrating again to obtain the lycopodium extract; The preparation method of the extract of *Hemerocallis fulva* includes the following steps: cleaning and removing impurities from the whole plant or roots of *Hemerocallis fulva*, mixing with 40-70% ethanol, and reflux extraction; after extraction, recovering the ethanol until there is no alcohol odor, then concentrating the extract, clarifying, filtering, and concentrating again to obtain the extract of *Hemerocallis fulva*.

2. The antioxidant repair composition according to claim 1, characterized in that, It includes the following components by weight: 1 part bamboo fungus extract, 1 part lycopodium extract, and 1 part chamaedorea extract.

3. The antioxidant repair composition according to claim 1, characterized in that, It also includes the following components by weight: 0.1-0.5 parts of Artemisia argyi.

4. A method for preparing the antioxidant repair composition as described in claim 3, characterized in that, Includes the following steps: Weigh out the water-extracted and alcohol-precipitated extract of bamboo fungus or the alcohol-extracted extract of bamboo fungus, the extract of lycopodium clavatum, the extract of chrysanthemum simonii, and the artemisia argyi according to the weight parts, mix them evenly, and the antioxidant repair composition is obtained.

5. A cosmetic product, characterized in that, Includes the antioxidant repair composition according to any one of claims 1-3.

6. The cosmetic product as described in claim 5, characterized in that, It also includes a humectant, a preservative, and water; the humectant is at least one of glycerin, 1,3-butanediol, and propylene glycol; the preservative is a compound of 1,2-hexanediol and octyl glycol.

7. The cosmetic product as described in claim 5 or 6, characterized in that, It comprises the following components by weight percentage: 3%-15% antioxidant repair composition, 5%-50% glycerin, 0%-5% 1,3-butanediol, 0%-45% propylene glycol, 0.525% 1,2-hexanediol, 0.175% octyl glycol, and water as the balance.

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